Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,588,160 A→G F128S (TTC→TCC)  ugpA ← UgpA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,588,1600AG98.9% 1869.2 / 3.1 548F128S (TTC→TCC) ugpAUgpA
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base G (247/295);  total (250/298)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.92e-01

Only 100 of 563 total aligned reads displayed.

CATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGAAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACT  >  NC_000913/3588069‑3588250
                                                                                           |                                                                                          
cATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAg                                                                                          <  1:10025968/94‑1 (MQ=255)
cATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAg                                                                                          >  2:7249221/1‑94 (MQ=255)
    gCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATcc                                                                                      >  1:10390355/1‑94 (MQ=255)
    gCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATcc                                                                                      >  2:2556255/1‑94 (MQ=255)
     ccTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCca                                                                                     <  2:5708778/94‑1 (MQ=255)
      cTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCcac                                                                                    >  2:4626406/1‑94 (MQ=255)
      cTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCcac                                                                                    <  1:7748506/94‑1 (MQ=255)
      cTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCcac                                                                                    >  2:10602035/1‑94 (MQ=255)
       tGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCcaca                                                                                   <  1:4373978/94‑1 (MQ=255)
         aCCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAAt                                                                                 <  2:6564758/94‑1 (MQ=255)
         aCCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAAt                                                                                 <  2:1622197/94‑1 (MQ=255)
          ccGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATa                                                                                <  1:5737733/94‑1 (MQ=255)
          ccGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATa                                                                                >  1:6162230/1‑94 (MQ=255)
          ccGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATa                                                                                <  1:268009/94‑1 (MQ=255)
          ccGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATa                                                                                <  1:8513588/94‑1 (MQ=255)
           cGCTGTTTGGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATAc                                                                               <  2:594685/94‑1 (MQ=255)
            gCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATAcg                                                                              <  1:6832212/94‑1 (MQ=255)
            gCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATAcg                                                                              <  1:782748/94‑1 (MQ=255)
              tGTTTGGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGACATCCACAATAcggc                                                                            <  1:8209103/94‑1 (MQ=255)
               gTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGATATCCACAATAcggcg                                                                           <  1:209336/94‑1 (MQ=255)
               gTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATAcggcg                                                                           <  2:6388835/94‑1 (MQ=255)
               gTTTTGCGCGGGGTTCCAGTCGTAGCCAAACTCCGCGAGAAAATCGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCCCAATAcggcg                                                                           <  2:8849500/94‑1 (MQ=255)
                 tttGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACggcggc                                                                         >  1:6291136/1‑94 (MQ=255)
                 tttGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACggcggc                                                                         <  2:4545574/94‑1 (MQ=255)
                  ttGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCa                                                                        <  2:2266323/94‑1 (MQ=255)
                  ttGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCa                                                                        <  1:3913113/94‑1 (MQ=255)
                    gcgcGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAc                                                                      >  1:736947/1‑94 (MQ=255)
                    gcgcGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAc                                                                      <  2:2845419/94‑1 (MQ=255)
                      gcgTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAcgg                                                                    <  1:10101070/94‑1 (MQ=255)
                       cgTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAcggc                                                                   <  1:4850087/94‑1 (MQ=255)
                         tGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAcggcgg                                                                 <  1:1816592/94‑1 (MQ=255)
                         tGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAcggcgg                                                                 <  1:10272148/94‑1 (MQ=255)
                         tGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAAcggcgg                                                                 <  1:4717180/94‑1 (MQ=255)
                          ggTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCggg                                                                <  2:7112923/94‑1 (MQ=255)
                          ggTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCggg                                                                >  2:8118144/1‑94 (MQ=255)
                           gTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCgggg                                                               >  1:1938012/1‑93 (MQ=255)
                            ttCCAGTCGTAGCCGCACTCGGCGAGAAAATGGGTGATCCGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAg                                                              <  2:7983590/94‑1 (MQ=255)
                            ttCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCggggg                                                              >  2:10153108/1‑92 (MQ=255)
                              ccAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGcc                                                            <  1:2440388/94‑1 (MQ=255)
                               cAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCa                                                           >  1:8919351/1‑94 (MQ=255)
                                aGTCGTAGCCGACCTCCGCGAGAAAGTGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCAc                                                          <  1:427341/94‑1 (MQ=255)
                                 gTCGTAGCCGACCTACGCGAGAAAATGGGTGATCAGCCGGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACg                                                         <  1:1545509/94‑1 (MQ=255)
                                 gTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACg                                                         <  2:901243/94‑1 (MQ=255)
                                  tCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACgg                                                        >  1:2259332/1‑94 (MQ=255)
                                  tCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACgg                                                        <  1:5897199/94‑1 (MQ=255)
                                  tCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACgg                                                        <  1:7523749/94‑1 (MQ=255)
                                   cGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGc                                                       <  1:9579535/94‑1 (MQ=255)
                                    gTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGGCACGGCg                                                      >  1:6544163/1‑94 (MQ=255)
                                     tAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGGCACGGCgg                                                     >  1:6445464/1‑93 (MQ=255)
                                      aGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCgga                                                    >  2:387575/1‑92 (MQ=255)
                                       gCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTaa                                                   <  1:4589552/94‑1 (MQ=255)
                                        ccGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCACGGCGGAAg                                                  >  2:9394120/1‑94 (MQ=255)
                                          gAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACCGCGGAAGgc                                                >  2:748514/1‑94 (MQ=255)
                                          gAACCCCGCGAGAAAATTGGTGATCAGCCCGCGACCGGGGCTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGTGCCCCGGCGTAAAgc                                                >  1:9418239/1‑94 (MQ=255)
                                            aCTCCGCGAGAAAATTGCTGATCAGCCCGCGACCGGGGTTAAACAGGAAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGgcag                                              <  1:10541968/94‑1 (MQ=255)
                                            aCTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCACGGCGTAAGgcag                                              >  2:127912/1‑94 (MQ=255)
                                            aCTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGgcag                                              <  2:3676616/94‑1 (MQ=255)
                                            aCTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGgcag                                              <  1:2994570/94‑1 (MQ=255)
                                               ccGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCaa                                           <  1:9855493/94‑1 (MQ=255)
                                                cgcgAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCACGGCGTGAGGGAGCAAc                                          >  1:3271102/1‑94 (MQ=255)
                                                cgcgAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAAc                                          <  1:3432513/94‑1 (MQ=255)
                                                cgcgAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAAc                                          <  1:5572465/94‑1 (MQ=255)
                                                cgcgAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAAc                                          >  2:4031685/1‑94 (MQ=255)
                                                  cgAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCACGGCGTAAGGCAGGAAAAt                                        >  2:237659/1‑94 (MQ=255)
                                                  cgAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACAt                                        <  1:9074996/94‑1 (MQ=255)
                                                  cgAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACAt                                        <  2:2713370/94‑1 (MQ=255)
                                                  cgAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGGAAGGCAGCAAcct                                        >  2:8385273/1‑92 (MQ=255)
                                                   gagaAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACAtt                                       <  2:9666111/94‑1 (MQ=255)
                                                    agaAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGGAAGGCAGCAACATTa                                      >  2:4072132/1‑94 (MQ=255)
                                                     gacaaTGGGCGATCAGCGCGTGCCGGGGGGTACACAGGGAGATACACAACTCGGCGCCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTaa                                     <  2:341633/91‑1 (MQ=255)
                                                      aaaaTGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAg                                    <  2:6693767/94‑1 (MQ=255)
                                                        aaTGGGTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGt                                  >  2:5182581/1‑94 (MQ=255)
                                                           gggTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCCCGGCGGAAGGCAGCAACATTAAGGTTTg                               >  1:8760262/1‑94 (MQ=255)
                                                           gggTGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGGAAGGCAGCAAAATTAAGGTTTg                               >  1:1236673/1‑94 (MQ=255)
                                                              tGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGGCACGGCGGAAGGCAGCAACATTAAGGGTTGATa                            >  1:5232698/1‑94 (MQ=255)
                                                              tGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATa                            <  1:7014969/94‑1 (MQ=255)
                                                              tGATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGGAAGGCAGCAACATTAAGGTTTGATa                            >  2:4361960/1‑94 (MQ=255)
                                                               gATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGGGCCACGGCGTAAGGCAGCAACATTAAGGTTTGAtta                           >  2:5423457/1‑92 (MQ=255)
                                                               gATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGGCACGGCGGAAGGGCGCAACATTAAGGGTTGATaa                           >  2:7879316/1‑93 (MQ=255)
                                                               gATCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAg                           <  1:3620781/94‑1 (MQ=255)
                                                                aTCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGa                          <  1:9213406/94‑1 (MQ=255)
                                                                 ttaccccGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGaa                         <  2:322070/90‑1 (MQ=255)
                                                                 tCAGCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGaa                         <  2:1754574/94‑1 (MQ=255)
                                                                    gCCCGCGAGCGGGCGTGAACAGGGAGATCCACAATACGGCGGCAACGGCTGGGGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAAct                      <  1:8161325/94‑2 (MQ=255)
                                                                    gCCCGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACg                      <  1:9720523/94‑1 (MQ=255)
                                                                      ccGCGACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAAAATTAAGGGTTGATAGAAACggg                    >  1:3113725/1‑93 (MQ=255)
                                                                        gcgACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTg                  <  1:6475212/94‑1 (MQ=255)
                                                                         cgACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCCACATTAAGGGTTGATAGAAACGGCTGc                 >  2:5004220/1‑94 (MQ=255)
                                                                         cgACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTTAGGGTTGATAGAAACGGCTGc                 >  1:415136/1‑94 (MQ=255)
                                                                         cgACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGc                 <  2:4789891/94‑1 (MQ=255)
                                                                          gACCGGGGTTAAACAGGGAGATCCACAATACGGCGGTAACGGCGGGAGGCACGGCGGCCGGCAGCAACCTTAAGGGTTGATAGAAAAGGcgggc                >  2:7663419/1‑90 (MQ=255)
                                                                          gACCGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGGTTGATAGAAACGGCTggc                >  2:1180278/1‑92 (MQ=255)
                                                                            ccGGGGTTAACCAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCcgc              <  1:6036238/94‑1 (MQ=255)
                                                                            ccGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCcgc              <  2:8741584/94‑1 (MQ=255)
                                                                             cGGGGTTAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCCACATTAAGGGTTGATAGAAACGGCTGCcgcg             >  1:1710991/1‑94 (MQ=255)
                                                                                   tAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGAt       <  2:10251118/94‑1 (MQ=255)
                                                                                   tAAACAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGAt       <  2:9286708/94‑1 (MQ=255)
                                                                                      aCAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTa    <  2:3003754/94‑1 (MQ=255)
                                                                                      aCAGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTa    <  2:2030415/94‑1 (MQ=255)
                                                                                        aGGGAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACt  <  2:1918876/94‑1 (MQ=255)
                                                                                           |                                                                                          
CATTGCCTGACCGCTGTTTTGCGCGTGGTTCCAGTCGTAGCCGAACTCCGCGAGAAAATGGGTGATCAGCCCGCGACCGGGGTTAAACAGGAAGATCCACAATACGGCGGCAACGGCGGGAGCCACGGCGTAAGGCAGCAACATTAAGGTTTGATAGAAACGGCTGCCGCGCACGATGTACT  >  NC_000913/3588069‑3588250

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 339 total aligned reads displayed.
CCTGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGTTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCAC  >  NC_000913/3591387‑3591573
                                                                                             |                                                                                             
CCTGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGC                                                                                               >  SRR4302545.10801267/1‑94 (MQ=60)
 CTGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCT                                                                                              <  SRR4302545.5620733/94‑1 (MQ=60)
  TGACGGTTGGGGTGTTCCAGCAAACCACTGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTT                                                                                             <  SRR4302545.8573063/94‑1 (MQ=60)
  TGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTT                                                                                             >  SRR4302545.7691063/1‑94 (MQ=60)
  TGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTT                                                                                             <  SRR4302545.8806160/94‑1 (MQ=60)
   GACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTT                                                                                            <  SRR4302545.6272091/94‑1 (MQ=60)
   GACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTT                                                                                            <  SRR4302545.5563490/94‑1 (MQ=60)
   GACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTT                                                                                            <  SRR4302545.2650740/94‑1 (MQ=60)
    ACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTC                                                                                           <  SRR4302545.6151316/94‑1 (MQ=60)
      GGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAA                                                                                         >  SRR4302545.3625756/1‑94 (MQ=60)
      GGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAA                                                                                         >  SRR4302545.3988706/1‑94 (MQ=60)
      GGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAA                                                                                         >  SRR4302545.5478568/1‑94 (MQ=60)
       GTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGCTGGCGCTTTCAca                                                                                        >  SRR4302545.9337759/1‑92 (MQ=60)
       GTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAAC                                                                                        <  SRR4302545.5166625/94‑1 (MQ=60)
       GTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGACGCGGTCGAGCGCTTCGCTCTCGGCGCGCCGGAAGGATAGCGCTTTCAAC                                                                                        >  SRR4302545.511686/1‑94 (MQ=60)
         TGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAG                                                                                      >  SRR4302545.7032729/1‑94 (MQ=60)
          GGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGG                                                                                     <  SRR4302545.6691446/94‑1 (MQ=60)
            CGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCC                                                                                   <  SRR4302545.6598141/94‑1 (MQ=60)
             GTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCA                                                                                  >  SRR4302545.6647387/1‑94 (MQ=60)
             GTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGACA                                                                                  >  SRR4302545.933099/1‑94 (MQ=60)
              TGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAG                                                                                 >  SRR4302545.3558762/1‑94 (MQ=60)
               GTTCCAGCAACCCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGA                                                                                <  SRR4302545.9555440/94‑1 (MQ=60)
               GTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGA                                                                                <  SRR4302545.482393/94‑1 (MQ=60)
               GTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGA                                                                                >  SRR4302545.7628633/1‑94 (MQ=60)
                gcacagacAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAG                                                                               <  SRR4302545.3433523/86‑1 (MQ=60)
                TTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAG                                                                               <  SRR4302545.162179/94‑1 (MQ=60)
                TTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAG                                                                               <  SRR4302545.6749492/94‑1 (MQ=60)
                 TCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGA                                                                              >  SRR4302545.4505326/1‑94 (MQ=60)
                 TCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGA                                                                              <  SRR4302545.3957705/94‑1 (MQ=60)
                 TCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGA                                                                              <  SRR4302545.3914224/94‑1 (MQ=60)
                  CCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAA                                                                             <  SRR4302545.3098193/94‑1 (MQ=60)
                   CAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAAC                                                                            >  SRR4302545.10024505/1‑94 (MQ=60)
                   CAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAAC                                                                            >  SRR4302545.10054701/1‑94 (MQ=60)
                   CAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAAC                                                                            <  SRR4302545.10170845/94‑1 (MQ=60)
                    AGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACA                                                                           <  SRR4302545.3311661/94‑1 (MQ=60)
                    AGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACA                                                                           >  SRR4302545.8244478/1‑94 (MQ=60)
                     GCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAG                                                                          >  SRR4302545.1684836/1‑94 (MQ=60)
                     GAAAAGCAATGCGCTGAAGCCAGGTCGCGGCTCGGTCGAGCGCTTCGCTCTGGGCGCGACTGAAGGATGGCGCTTTCAACAGGCCAGAGAACAG                                                                          <  SRR4302545.8684417/94‑1 (MQ=60)
                      CAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGC                                                                         >  SRR4302545.1472685/1‑94 (MQ=60)
                      CAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGC                                                                         >  SRR4302545.8840217/1‑94 (MQ=60)
                       AAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCC                                                                        >  SRR4302545.6656567/1‑94 (MQ=60)
                       AAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCC                                                                        >  SRR4302545.419636/1‑94 (MQ=60)
                        ACCCAATGGGCTCAAGCCCGGTCGCGGTGCTGTCGAGCGCTTCGCTCTGGGCGCGCCGGAAGGATGGCGCTTTCAACAGGCCAGAGAACACCCC                                                                       <  SRR4302545.7394100/94‑1 (MQ=60)
                        ACCCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCC                                                                       <  SRR4302545.7540822/94‑1 (MQ=60)
                        AACGAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGCATGGCGCTTTCAACAGGCCAGAGAACAGCCC                                                                       <  SRR4302545.4867428/94‑1 (MQ=60)
                        AACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCC                                                                       <  SRR4302545.6073713/94‑1 (MQ=60)
                        AACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCC                                                                       <  SRR4302545.8370310/94‑1 (MQ=60)
                        AACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCC                                                                       <  SRR4302545.8864491/94‑1 (MQ=60)
                        AACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCC                                                                       <  SRR4302545.9144559/94‑1 (MQ=60)
                         ACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCG                                                                      <  SRR4302545.2308656/94‑1 (MQ=60)
                          CCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGTCGGAAGGATGGCGCTTTCAACAGCCCAGGAAACAGCCCGG                                                                     >  SRR4302545.9302307/1‑94 (MQ=60)
                          CCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGG                                                                     >  SRR4302545.1924163/1‑94 (MQ=60)
                           CAATGCTCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGT                                                                    <  SRR4302545.9465799/94‑1 (MQ=60)
                           CAATGCGCTCAAACCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGT                                                                    >  SRR4302545.6125772/1‑94 (MQ=60)
                            AATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTT                                                                   >  SRR4302545.5872919/1‑94 (MQ=60)
                            AATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTT                                                                   <  SRR4302545.10233948/94‑1 (MQ=60)
                             ATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTT                                                                  >  SRR4302545.4852506/1‑94 (MQ=60)
                             ATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTT                                                                  <  SRR4302545.7746575/94‑1 (MQ=60)
                             ATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTT                                                                  >  SRR4302545.7846922/1‑94 (MQ=60)
                             ATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTT                                                                  >  SRR4302545.63066/1‑94 (MQ=60)
                             ATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTT                                                                  <  SRR4302545.10936991/94‑1 (MQ=60)
                               GCGCTCAAGCCAGTTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCTCTTTCAACAGGCCAGAGAACAGCCCGGTTTTC                                                                <  SRR4302545.2776468/94‑1 (MQ=60)
                               GCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTC                                                                >  SRR4302545.5253579/1‑94 (MQ=60)
                                CGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCA                                                               >  SRR4302545.4333804/1‑94 (MQ=60)
                                CGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCA                                                               <  SRR4302545.969649/94‑1 (MQ=60)
                                 GCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAG                                                              <  SRR4302545.7596080/94‑1 (MQ=60)
                                  CTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGT                                                             <  SRR4302545.9333250/94‑1 (MQ=60)
                                  CTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGT                                                             >  SRR4302545.5357352/1‑94 (MQ=60)
                                  CTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGT                                                             >  SRR4302545.2271389/1‑94 (MQ=60)
                                   TCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTT                                                            >  SRR4302545.1904512/1‑94 (MQ=60)
                                   TCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTT                                                            >  SRR4302545.5027574/1‑94 (MQ=60)
                                   TCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTT                                                            >  SRR4302545.6537391/1‑94 (MQ=60)
                                   TCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACACCCCGGTTTTCAGTT                                                            <  SRR4302545.10022519/94‑1 (MQ=60)
                                    CAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTG                                                           <  SRR4302545.4456869/94‑1 (MQ=60)
                                    CAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTG                                                           <  SRR4302545.2825785/94‑1 (MQ=60)
                                    CAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTG                                                           >  SRR4302545.3659461/1‑94 (MQ=60)
                                      AGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCT                                                         >  SRR4302545.5669113/1‑94 (MQ=60)
                                      AGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCT                                                         >  SRR4302545.1353952/1‑94 (MQ=60)
                                       GCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTG                                                        <  SRR4302545.7523433/94‑1 (MQ=60)
                                       GCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTG                                                        <  SRR4302545.319640/94‑1 (MQ=60)
                                       GCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTG                                                        >  SRR4302545.7505897/1‑94 (MQ=60)
                                       GCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTG                                                        <  SRR4302545.2705028/94‑1 (MQ=60)
                                       GCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTG                                                        <  SRR4302545.5049479/94‑1 (MQ=60)
                                       GCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTG                                                        <  SRR4302545.7091149/94‑1 (MQ=60)
                                        agcatcagcaactgaaaaccgggctgttCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGA                                                       >  SRR4302545.1918379/29‑94 (MQ=60)
                                        CCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGA                                                       >  SRR4302545.8648571/1‑94 (MQ=60)
                                        CCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTAGGGGCGACCGAAGGAATGCGCTTTCATCACGCCAGAGAATAGCCCGGTTTTCAGTTGCTGA                                                       >  SRR4302545.5772840/1‑94 (MQ=53)
                                         CAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGAT                                                      >  SRR4302545.2325712/1‑94 (MQ=60)
                                         CAGGTCGCGGCGCGCTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACATCCCGGTTTTCAGTTGCTGAT                                                      <  SRR4302545.1503945/94‑1 (MQ=60)
                                          AGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATG                                                     >  SRR4302545.3122698/1‑94 (MQ=60)
                                          AGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATG                                                     <  SRR4302545.3438910/94‑1 (MQ=60)
                                          AGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATG                                                     >  SRR4302545.4137914/1‑94 (MQ=60)
                                          AGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATG                                                     <  SRR4302545.565483/94‑1 (MQ=60)
                                          AGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATG                                                     >  SRR4302545.7194089/1‑94 (MQ=60)
                                          AGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATG                                                     <  SRR4302545.9031813/94‑1 (MQ=60)
                                           GGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGC                                                    >  SRR4302545.2887192/1‑94 (MQ=60)
                                           GGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGC                                                    >  SRR4302545.6798723/1‑94 (MQ=60)
                                           GGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGC                                                    >  SRR4302545.10874073/1‑94 (MQ=60)
                                           GGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGC                                                    <  SRR4302545.9060476/94‑1 (MQ=60)
                                           GGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGC                                                    >  SRR4302545.5088828/1‑94 (MQ=60)
                                            GTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCT                                                   <  SRR4302545.845863/94‑1 (MQ=60)
                                            GTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCT                                                   <  SRR4302545.938073/94‑1 (MQ=60)
                                            GTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCT                                                   <  SRR4302545.7942180/94‑1 (MQ=60)
                                            GTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCT                                                   <  SRR4302545.2005208/94‑1 (MQ=60)
                                            GTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCT                                                   >  SRR4302545.10150859/1‑94 (MQ=60)
                                            GTCGCGGCGCGGGCGAGCGCTGCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCT                                                   <  SRR4302545.7165685/94‑1 (MQ=60)
                                             TCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTG                                                  <  SRR4302545.7497595/94‑1 (MQ=60)
                                               GCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCC                                                >  SRR4302545.8847648/1‑94 (MQ=60)
                                                CGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGC                                               >  SRR4302545.623391/1‑94 (MQ=60)
                                                 GGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTTAACAGGCCAGAGAACAGCCCGGCTTTCAGATCCTGCTGCTGCGCC                                              >  SRR4302545.7258389/1‑94 (MQ=60)
                                                 GGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCC                                              >  SRR4302545.3026532/1‑94 (MQ=60)
                                                    GCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACC                                           >  SRR4302545.603119/1‑94 (MQ=60)
                                                    GCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACC                                           >  SRR4302545.5281997/1‑94 (MQ=60)
                                                     GGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCA                                          <  SRR4302545.1699837/94‑1 (MQ=60)
                                                     CGGTCGAGCGCTTCGCTCTGGGGGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCA                                          >  SRR4302545.6862179/1‑94 (MQ=60)
                                                      GGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAG                                         >  SRR4302545.3854408/1‑94 (MQ=60)
                                                       GTCGAGCGCTTCGCTGTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGC                                        <  SRR4302545.9140237/94‑1 (MQ=60)
                                                         CGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAG                                      <  SRR4302545.8102903/94‑1 (MQ=60)
                                                          GAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGG                                     <  SRR4302545.7653471/94‑1 (MQ=60)
                                                          GAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGG                                     >  SRR4302545.4178137/1‑94 (MQ=60)
                                                          GAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGG                                     >  SRR4302545.8583428/1‑94 (MQ=60)
                                                          GAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGG                                     >  SRR4302545.647309/1‑94 (MQ=60)
                                                           AGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACATCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGT                                    <  SRR4302545.2507420/94‑1 (MQ=60)
                                                           AGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGT                                    >  SRR4302545.790402/1‑94 (MQ=60)
                                                           AGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCGTTCAACAGGCCAGAGAACAGCCTGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGT                                    <  SRR4302545.4487074/94‑1 (MQ=60)
                                                             CGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTT                                  <  SRR4302545.632837/94‑1 (MQ=60)
                                                              GCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTT                                 <  SRR4302545.6025198/94‑1 (MQ=60)
                                                              GCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTT                                 <  SRR4302545.8540211/94‑1 (MQ=60)
                                                              GCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTT                                 >  SRR4302545.2325981/1‑94 (MQ=60)
                                                               CTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTC                                >  SRR4302545.8674996/1‑94 (MQ=60)
                                                               CTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTC                                <  SRR4302545.7286321/94‑1 (MQ=60)
                                                               CTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTC                                >  SRR4302545.10648530/1‑94 (MQ=60)
                                                                TTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCA                               <  SRR4302545.1254629/94‑1 (MQ=60)
                                                                  CGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAAT                             >  SRR4302545.224030/1‑94 (MQ=60)
                                                                  CGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAAT                             <  SRR4302545.8188719/94‑1 (MQ=60)
                                                                  CGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAAT                             >  SRR4302545.7348878/1‑94 (MQ=60)
                                                                   GCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATT                            <  SRR4302545.3110064/94‑1 (MQ=60)
                                                                   GCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATT                            <  SRR4302545.4292980/94‑1 (MQ=60)
                                                                    CTCTGGGCGCGACGGAAGGGTGCCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTTCGCCACCAGCAGGTTTTCAATTA                           >  SRR4302545.8892177/1‑94 (MQ=60)
                                                                    CTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTA                           >  SRR4302545.9066735/1‑94 (MQ=60)
                                                                    CTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTA                           >  SRR4302545.8892176/1‑94 (MQ=60)
                                                                      CTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACC                         <  SRR4302545.1982385/94‑1 (MQ=60)
                                                                        GGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGT                       >  SRR4302545.9222998/1‑94 (MQ=60)
                                                                        GGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGT                       <  SRR4302545.2413519/94‑1 (MQ=60)
                                                                         GGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTC                      <  SRR4302545.3770969/94‑1 (MQ=60)
                                                                          GCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCA                     >  SRR4302545.245959/1‑94 (MQ=60)
                                                                          GCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCA                     >  SRR4302545.2746840/1‑94 (MQ=60)
                                                                            GCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATT                   <  SRR4302545.9345747/94‑1 (MQ=60)
                                                                            GCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATT                   <  SRR4302545.1492434/94‑1 (MQ=60)
                                                                            GCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATT                   <  SRR4302545.3642873/94‑1 (MQ=60)
                                                                             CGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTT                  <  SRR4302545.10557421/94‑1 (MQ=60)
                                                                             CGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTT                  >  SRR4302545.4230230/1‑94 (MQ=60)
                                                                             CGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTT                  >  SRR4302545.2090324/1‑94 (MQ=60)
                                                                              GACGGAAGGGTGGCGCTTTCAACAGGCCAGAGAACAGGCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTATCGTCATTTT                 >  SRR4302545.9541719/1‑94 (MQ=60)
                                                                              GACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTC                 <  SRR4302545.5913174/94‑1 (MQ=60)
                                                                              GACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTC                 <  SRR4302545.6965222/94‑1 (MQ=60)
                                                                               ACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCA                <  SRR4302545.10275880/94‑1 (MQ=60)
                                                                               ACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCA                <  SRR4302545.4611789/94‑1 (MQ=60)
                                                                               ACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCA                <  SRR4302545.2012490/94‑1 (MQ=60)
                                                                               ACAGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCA                <  SRR4302545.10275879/94‑1 (MQ=60)
                                                                                CGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCAC               <  SRR4302545.258328/94‑1 (MQ=60)
                                                                                CGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCAC               <  SRR4302545.5477044/94‑1 (MQ=60)
                                                                                CGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCAC               >  SRR4302545.10637580/1‑94 (MQ=60)
                                                                                 GGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACG              <  SRR4302545.3684402/94‑1 (MQ=60)
                                                                                 GGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACG              <  SRR4302545.6085952/94‑1 (MQ=60)
                                                                                 GGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACG              <  SRR4302545.1832947/94‑1 (MQ=60)
                                                                                 GGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACG              >  SRR4302545.1132389/1‑94 (MQ=60)
                                                                                  GAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGG             <  SRR4302545.8255799/94‑1 (MQ=60)
                                                                                   AAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGA            >  SRR4302545.10555655/1‑94 (MQ=60)
                                                                                   AAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGA            >  SRR4302545.7040152/1‑94 (MQ=60)
                                                                                    AGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAA           >  SRR4302545.1452250/1‑94 (MQ=60)
                                                                                    AGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAA           <  SRR4302545.8544078/94‑1 (MQ=60)
                                                                                    AGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAA           >  SRR4302545.7578747/1‑94 (MQ=60)
                                                                                    AGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAA           <  SRR4302545.5992894/94‑1 (MQ=60)
                                                                                     GGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAAC          >  SRR4302545.2897017/1‑94 (MQ=60)
                                                                                     GGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAAC          >  SRR4302545.7353241/1‑94 (MQ=60)
                                                                                      GATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACA         >  SRR4302545.10496921/1‑94 (MQ=60)
                                                                                      GATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACA         >  SRR4302545.9912848/1‑94 (MQ=60)
                                                                                      GATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACA         <  SRR4302545.10865995/94‑1 (MQ=60)
                                                                                      GATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACA         <  SRR4302545.4555587/94‑1 (MQ=60)
                                                                                      GATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGATTTCAGTTGCTGATGCTGCGCCACCAGCAGGCTTTCAATTACCGTCATTTCACGGAACA         >  SRR4302545.6804273/1‑94 (MQ=60)
                                                                                       ATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAG        >  SRR4302545.1299616/1‑94 (MQ=60)
                                                                                       ATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAG        >  SRR4302545.10369689/1‑94 (MQ=60)
                                                                                       ATGGCGCTTTAAACAGGCGAGAGACCCCCCCGGTTATCAGTTGTTGATGCTGCGCCACCAGCAGGTTTGCAATTACCGCCATTTCTCGGTACAG        <  SRR4302545.6730274/94‑1 (MQ=28)
                                                                                        TGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGA       <  SRR4302545.2492318/94‑1 (MQ=60)
                                                                                        TGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGA       >  SRR4302545.9058950/1‑94 (MQ=60)
                                                                                         GGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGAC      >  SRR4302545.11415/1‑94 (MQ=60)
                                                                                         GGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGAC      >  SRR4302545.2260253/1‑94 (MQ=60)
                                                                                          GCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACG     <  SRR4302545.3370520/94‑1 (MQ=60)
                                                                                            GCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCA   <  SRR4302545.6639345/94‑1 (MQ=60)
                                                                                            GCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCA   >  SRR4302545.8273727/1‑94 (MQ=60)
                                                                                            GCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCA   >  SRR4302545.4366615/1‑94 (MQ=60)
                                                                                            GCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGGTTTCAATTACCGTCATTTCACGGAACAGACGCA   >  SRR4302545.6844246/1‑94 (MQ=60)
                                                                                             CTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCAC  <  SRR4302545.6239680/94‑1 (MQ=60)
                                                                                             CTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCAC  >  SRR4302545.1049286/1‑94 (MQ=60)
                                                                                             CTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCAC  >  SRR4302545.8147410/1‑94 (MQ=60)
                                                                                             CTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCAC  >  SRR4302545.5500350/1‑94 (MQ=60)
                                                                                             CTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCAC  >  SRR4302545.4641970/1‑94 (MQ=60)
                                                                                             CTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCAC  <  SRR4302545.3236662/94‑1 (MQ=60)
                                                                                             CTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCAC  >  SRR4302545.8527350/1‑94 (MQ=60)
                                                                                             |                                                                                             
CCTGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGTTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCAC  >  NC_000913/3591387‑3591573

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑