Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,591,480 T→C T120A (ACG→GCG)  livG ← ATP‑binding component of high‑affinity branched‑chain amino acid transport system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,591,4800TC100.0% 871.0 / NA 249T120A (ACG→GCG) livGATP‑binding component of high‑affinity branched‑chain amino acid transport system
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (98/151);  total (98/151)

Only 100 of 270 total aligned reads displayed.

GACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGTTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGAC  >  NC_000913/3591390‑3591569
                                                                                          |                                                                                         
gACGGTTGGCGTGTTCCAGCAAACCAATGTGCTCAAGCCAGGTCGCGGCGCGGTCGAGGGCTTCGCTCTGGGCGCGACGGAAGGATGGCGctt                                                                                         >  2:5818868/1‑93 (MQ=255)
gACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGctt                                                                                         >  1:4570819/1‑93 (MQ=255)
gACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGctt                                                                                         <  2:5880628/93‑1 (MQ=255)
  cGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTc                                                                                       <  1:4033641/93‑1 (MQ=255)
    gTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCaa                                                                                     <  1:1007295/93‑1 (MQ=255)
    gTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCaa                                                                                     >  2:1201100/1‑93 (MQ=255)
    gTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCaa                                                                                     >  2:195154/1‑93 (MQ=255)
    gTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGGCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCaa                                                                                     >  1:937316/1‑93 (MQ=255)
        gCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAgg                                                                                 >  1:137595/1‑93 (MQ=255)
         cGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGc                                                                                <  2:5025340/93‑1 (MQ=255)
          gtgtTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGcc                                                                               >  1:2061137/1‑93 (MQ=255)
          gtgtTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGcc                                                                               <  2:1699287/93‑1 (MQ=255)
           tgtTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCa                                                                              <  2:3056692/93‑1 (MQ=255)
           tgtTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCa                                                                              <  2:1582288/93‑1 (MQ=255)
             ttCCAGCAAACCAATGCGCTCAAGTCAGGTCGCGGCGCGGTTGTGCGCTTCGCTCTGGGCGTTACGGAAGGATGGCGCTTTCAACAGGCCaga                                                                            >  2:6476492/1‑93 (MQ=255)
             ttCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCaga                                                                            <  1:6079059/93‑1 (MQ=255)
             ttCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCaga                                                                            <  1:4064946/93‑1 (MQ=255)
               ccAGCAACCCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAgaga                                                                          <  1:3210800/93‑1 (MQ=255)
               ccAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAgaga                                                                          >  1:2472691/1‑93 (MQ=255)
               ccAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAgaga                                                                          <  1:4098828/93‑1 (MQ=255)
               ccAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAgaga                                                                          >  2:1534069/1‑93 (MQ=255)
                cAGCAAGCGATTGCGCTCAAGCCAGGTCTCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGaa                                                                         <  1:1462667/93‑1 (MQ=255)
                cAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGaa                                                                         >  2:5157562/1‑93 (MQ=255)
                cAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGaa                                                                         >  2:2794919/1‑93 (MQ=255)
                cAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGaa                                                                         <  2:6004519/93‑1 (MQ=255)
                 aGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAAc                                                                        <  1:3768349/93‑1 (MQ=255)
                 aGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAAc                                                                        <  2:4133156/93‑1 (MQ=255)
                 aGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAAc                                                                        <  1:482877/93‑1 (MQ=255)
                     aaCCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGcc                                                                    <  1:6348436/93‑1 (MQ=255)
                     aaCCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGcc                                                                    >  1:5603683/1‑93 (MQ=255)
                     aaCCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGcc                                                                    >  2:945400/1‑93 (MQ=255)
                     aaCCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGcc                                                                    <  1:3360322/93‑1 (MQ=255)
                      aCCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGccc                                                                   <  1:179580/93‑1 (MQ=255)
                       ccAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCg                                                                  >  1:6254115/1‑93 (MQ=255)
                        cAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCgg                                                                 <  1:358906/93‑1 (MQ=255)
                         aaTGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGt                                                                >  1:109540/1‑93 (MQ=255)
                         aaTGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGt                                                                <  2:4551028/93‑1 (MQ=255)
                          aTGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGtt                                                               <  1:2604178/93‑1 (MQ=255)
                           tGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGttt                                                              <  1:5419262/93‑1 (MQ=255)
                             cgcTCAAGCCAGGTCGCGGCCCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTc                                                            <  1:1544371/93‑1 (MQ=255)
                              gcTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCa                                                           <  2:3218009/93‑1 (MQ=255)
                               cTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTCGGCGCGACGGAAGGAGGGCGCTTTCAACAGGCCGGAGAAGAGCCCGGTTTTCAg                                                          >  2:3694718/1‑93 (MQ=255)
                                tCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAATGATGGCGCTTTCAATAGGCCAGAGAACAGCCCGGTTTTCAGt                                                         <  1:4546316/93‑1 (MQ=255)
                                 cAAGCCACGTCGCGGCGCAGCCGAGCGCTTCCCTCTGGGCGCGACGGAAGGACGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGtt                                                        <  2:4312576/93‑1 (MQ=255)
                                   aGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGc                                                      <  1:4882268/93‑1 (MQ=255)
                                    gCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATTGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCt                                                     <  1:3459162/93‑1 (MQ=255)
                                      cAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGa                                                   >  2:5220770/1‑93 (MQ=255)
                                       cggTCGCGGCGGGGTCGAGCGCTTCGCTCTGGGCGCCACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGAt                                                  <  2:2252001/92‑1 (MQ=255)
                                          tCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGAtgct                                               <  2:2464639/93‑1 (MQ=255)
                                            gcggcgcggTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGAtgctgc                                             >  2:6434901/1‑93 (MQ=255)
                                            gcggcgcggTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGAtgctgc                                             >  2:4732157/1‑93 (MQ=255)
                                             cggcgcggTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCCGTTGCTGCtgatgtg                                            >  1:3327686/1‑91 (MQ=255)
                                               gcgcggTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGcc                                          >  1:887675/1‑93 (MQ=255)
                                                cgcggTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGCTGGCGCTTTCAACAGGCCAGAGAACAGCCCGGCTTTCAGTTGCTGATGCTGCGcca                                         >  2:3619907/1‑93 (MQ=255)
                                                 gcggTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGccac                                        >  1:4690365/1‑93 (MQ=255)
                                                 gcggTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGccac                                        <  1:4347367/93‑1 (MQ=255)
                                                  cggTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGccacc                                       <  1:4225772/93‑1 (MQ=255)
                                                   ggTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGccacca                                      <  1:5507555/93‑1 (MQ=255)
                                                    gTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACcag                                     >  2:5639041/1‑93 (MQ=255)
                                                    gTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACcag                                     <  2:2565345/93‑1 (MQ=255)
                                                    gTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACcag                                     >  1:2294160/1‑93 (MQ=255)
                                                     tCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACcagc                                    <  1:5364547/93‑1 (MQ=255)
                                                      cGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACcagca                                   <  1:3054675/93‑1 (MQ=255)
                                                       gAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACcagcag                                  <  1:2684112/93‑1 (MQ=255)
                                                         gcgcTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGt                                <  2:4444270/93‑1 (MQ=255)
                                                         gcgcTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGt                                >  2:5607582/1‑93 (MQ=255)
                                                           gcTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGttt                              >  1:5034843/1‑93 (MQ=255)
                                                            cTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGtttt                             >  2:12674/1‑93 (MQ=255)
                                                             ttCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTc                            >  1:881351/1‑93 (MQ=255)
                                                                gCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAAt                         <  1:1965033/93‑1 (MQ=255)
                                                                gCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAAt                         <  2:4807781/93‑1 (MQ=255)
                                                                gCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAAt                         >  2:1320647/1‑93 (MQ=255)
                                                                 ctctGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAAtt                        <  1:795540/93‑1 (MQ=255)
                                                                 ctctGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAAtt                        <  2:3984420/93‑1 (MQ=255)
                                                                  tctGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTa                       <  2:5453967/93‑1 (MQ=255)
                                                                  tctGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTa                       >  1:630408/1‑93 (MQ=255)
                                                                  tctGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTa                       >  2:24986/1‑93 (MQ=255)
                                                                  tctGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTa                       >  2:3412565/1‑93 (MQ=255)
                                                                   ctGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCTACCAGCAGGTTTTCAATTAc                      <  2:6222443/93‑1 (MQ=255)
                                                                   ctGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTAc                      <  2:1867190/93‑1 (MQ=255)
                                                                    tGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTAcc                     <  1:6127194/93‑1 (MQ=255)
                                                                     gggCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCg                    <  2:854607/93‑1 (MQ=255)
                                                                      ggCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGt                   >  2:4969608/1‑93 (MQ=255)
                                                                       gcgcGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTc                  <  2:3473641/93‑1 (MQ=255)
                                                                       gcgcGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTAAATTACCGTc                  <  1:1853421/93‑1 (MQ=255)
                                                                        cgcgACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCa                 <  2:3548118/93‑1 (MQ=255)
                                                                          cgACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCAtt               <  2:2094548/93‑1 (MQ=255)
                                                                           gACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCAttt              <  1:5668880/93‑1 (MQ=255)
                                                                             cGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCa            >  2:2976620/1‑93 (MQ=255)
                                                                              ggAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCAc           <  1:4172247/93‑1 (MQ=255)
                                                                              ggAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCAc           >  1:367823/1‑93 (MQ=255)
                                                                              ggAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCAc           <  2:6121887/93‑1 (MQ=255)
                                                                                 aGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTCTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGa        <  2:1069135/93‑1 (MQ=255)
                                                                                  ggATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGaa       >  1:1433779/1‑93 (MQ=255)
                                                                                   gATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAAc      <  2:4340273/93‑1 (MQ=255)
                                                                                   gATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAAc      >  2:3935705/1‑93 (MQ=255)
                                                                                    aTGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACa     >  2:3168842/1‑93 (MQ=255)
                                                                                    aTGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACa     >  2:5111283/1‑93 (MQ=255)
                                                                                    aTGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACa     <  2:442372/93‑1 (MQ=255)
                                                                                      ggCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCTCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGa   <  1:4224402/93‑1 (MQ=255)
                                                                                          |                                                                                         
GACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGTTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGAC  >  NC_000913/3591390‑3591569

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCTGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGTTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCA  >  NC_000913/3591387‑3591572
                                                                                             |                                                                                            
CCTGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGC                                                                                              >  SRR4302537.5910418/1‑94 (MQ=60)
CCTGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGC                                                                                              >  SRR4302537.5428369/1‑94 (MQ=60)
CCTGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGC                                                                                              >  SRR4302537.3535046/1‑94 (MQ=60)
 CTGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCT                                                                                             <  SRR4302537.2608222/94‑1 (MQ=60)
 CTGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCT                                                                                             >  SRR4302537.2080557/1‑94 (MQ=60)
 CTGACGGTTGGCGTGTTCCAGCAAACCAATCCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCT                                                                                             <  SRR4302537.5134312/94‑1 (MQ=60)
  TGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTT                                                                                            >  SRR4302537.4602456/1‑94 (MQ=60)
   GACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTT                                                                                           >  SRR4302537.3573185/1‑94 (MQ=60)
     CGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCA                                                                                         <  SRR4302537.4061873/94‑1 (MQ=60)
     CGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCA                                                                                         <  SRR4302537.3893808/94‑1 (MQ=60)
      GGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGGCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAA                                                                                        >  SRR4302537.943778/1‑94 (MQ=60)
       GTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAAC                                                                                       <  SRR4302537.1014248/94‑1 (MQ=60)
        TTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACA                                                                                      <  SRR4302537.5572707/94‑1 (MQ=60)
         TGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAG                                                                                     >  SRR4302537.1160475/1‑94 (MQ=60)
         TGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAG                                                                                     <  SRR4302537.1984930/94‑1 (MQ=60)
          TGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGG                                                                                    >  SRR4302537.4357170/1‑94 (MQ=60)
          GGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCCACAGG                                                                                    >  SRR4302537.2687057/1‑94 (MQ=60)
          GGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGG                                                                                    >  SRR4302537.138583/1‑94 (MQ=60)
           GCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGC                                                                                   <  SRR4302537.3223940/94‑1 (MQ=60)
            CGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCC                                                                                  >  SRR4302537.3422880/1‑94 (MQ=60)
            CGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCC                                                                                  >  SRR4302537.2075608/1‑94 (MQ=60)
            CGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCC                                                                                  <  SRR4302537.6233349/94‑1 (MQ=60)
            CGGGTCCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCC                                                                                  <  SRR4302537.4976693/94‑1 (MQ=60)
             GTGTTCCAGCAACCCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCA                                                                                 <  SRR4302537.2280178/94‑1 (MQ=60)
             GTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCA                                                                                 >  SRR4302537.4764991/1‑94 (MQ=60)
              TGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAG                                                                                >  SRR4302537.6346084/1‑94 (MQ=60)
               GTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGA                                                                               <  SRR4302537.4085555/94‑1 (MQ=60)
                TTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAG                                                                              <  SRR4302537.6120833/94‑1 (MQ=60)
                TTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAG                                                                              <  SRR4302537.4093349/94‑1 (MQ=60)
                 TCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGA                                                                             >  SRR4302537.2210412/1‑94 (MQ=60)
                 TCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGA                                                                             >  SRR4302537.2490075/1‑94 (MQ=60)
                  CCAGCAACCCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAA                                                                            <  SRR4302537.3233383/94‑1 (MQ=60)
                  CCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAA                                                                            >  SRR4302537.6155596/1‑94 (MQ=60)
                  CCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAA                                                                            <  SRR4302537.4127444/94‑1 (MQ=60)
                   CAGCAAGCGATTGCGCTCAAGCCAGGTCTCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAAC                                                                           <  SRR4302537.1472841/94‑1 (MQ=60)
                    AGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACA                                                                          <  SRR4302537.1431991/94‑1 (MQ=60)
                    AGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACA                                                                          <  SRR4302537.486184/94‑1 (MQ=60)
                    AGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACA                                                                          <  SRR4302537.3794806/94‑1 (MQ=60)
                    AGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACA                                                                          <  SRR4302537.3778426/94‑1 (MQ=60)
                     GCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAG                                                                         <  SRR4302537.1924287/94‑1 (MQ=60)
                      CAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGC                                                                        >  SRR4302537.3271323/1‑94 (MQ=60)
                       AAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCC                                                                       >  SRR4302537.4171802/1‑94 (MQ=60)
                       AAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCC                                                                       >  SRR4302537.6353968/1‑94 (MQ=60)
                       AAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCC                                                                       <  SRR4302537.2463602/94‑1 (MQ=60)
                       AAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCC                                                                       <  SRR4302537.713597/94‑1 (MQ=60)
                       AAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCC                                                                       >  SRR4302537.5641993/1‑94 (MQ=60)
                        AACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCC                                                                      <  SRR4302537.3384123/94‑1 (MQ=60)
                        AACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCC                                                                      <  SRR4302537.6391791/94‑1 (MQ=60)
                         ACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCG                                                                     >  SRR4302537.1158021/1‑94 (MQ=60)
                         ACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCG                                                                     <  SRR4302537.180859/94‑1 (MQ=60)
                         ACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCG                                                                     >  SRR4302537.6297078/1‑94 (MQ=60)
                          CCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGG                                                                    >  SRR4302537.5447621/1‑94 (MQ=60)
                           CAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGT                                                                   >  SRR4302537.110345/1‑94 (MQ=60)
                           CAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGT                                                                   <  SRR4302537.361398/94‑1 (MQ=60)
                             ATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTT                                                                 <  SRR4302537.2622390/94‑1 (MQ=60)
                              TGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTT                                                                <  SRR4302537.5456196/94‑1 (MQ=60)
                                CGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCA                                                              <  SRR4302537.1101639/94‑1 (MQ=60)
                                CGCTCAAGCCAGGTCGCGGCCCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCA                                                              <  SRR4302537.1555154/94‑1 (MQ=60)
                                 GCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAG                                                             >  SRR4302537.4120017/1‑94 (MQ=60)
                                  CTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGT                                                            >  SRR4302537.1468913/1‑94 (MQ=60)
                                   TCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAATGATGGCGCTTTCAATAGGCCAGAGAACAGCCCGGTTTTCAGTT                                                           <  SRR4302537.4577777/94‑1 (MQ=60)
                                   TCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTT                                                           <  SRR4302537.2561531/94‑1 (MQ=60)
                                    CAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTG                                                          >  SRR4302537.4186001/1‑94 (MQ=60)
                                      AGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCT                                                        <  SRR4302537.4915993/94‑1 (MQ=60)
                                      AGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGCGCGCGACGGAAGGCTGGCGCTTTCAACATGCCAGAGAACAGCCCGGTTTTCAGTTGCT                                                        >  SRR4302537.2877563/1‑94 (MQ=60)
                                       GCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATTGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTG                                                       <  SRR4302537.3483617/94‑1 (MQ=60)
                                         CAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGAT                                                     <  SRR4302537.1495581/94‑1 (MQ=60)
                                          AGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATG                                                    <  SRR4302537.1593/94‑1 (MQ=60)
                                              CGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGCTGCTGC                                                >  SRR4302537.4841391/1‑94 (MQ=60)
                                              CGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGC                                                >  SRR4302537.4532615/1‑94 (MQ=60)
                                              CGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGC                                                <  SRR4302537.6437163/94‑1 (MQ=60)
                                              CGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGC                                                >  SRR4302537.865466/1‑94 (MQ=60)
                                              CGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGC                                                >  SRR4302537.1001597/1‑94 (MQ=60)
                                               GCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCCGTTGCTGctgatgtg                                               >  SRR4302537.3351235/1‑86 (MQ=60)
                                               GCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCG                                               <  SRR4302537.2643077/94‑1 (MQ=60)
                                               GCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCG                                               <  SRR4302537.4206807/94‑1 (MQ=60)
                                               GCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCG                                               >  SRR4302537.454074/1‑94 (MQ=60)
                                                 GGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCC                                             >  SRR4302537.893781/1‑94 (MQ=60)
                                                  GCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCA                                            <  SRR4302537.1945434/94‑1 (MQ=60)
                                                   CGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCAC                                           >  SRR4302537.4722803/1‑94 (MQ=60)
                                                    GCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACC                                          <  SRR4302537.4377498/94‑1 (MQ=60)
                                                     CGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCA                                         <  SRR4302537.4255205/94‑1 (MQ=60)
                                                     CGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCA                                         >  SRR4302537.896265/1‑94 (MQ=60)
                                                      GGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAG                                        <  SRR4302537.5545169/94‑1 (MQ=60)
                                                      GGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAG                                        >  SRR4302537.2310356/1‑94 (MQ=60)
                                                      GGGCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAG                                        <  SRR4302537.4870083/94‑1 (MQ=60)
                                                        TCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCA                                      <  SRR4302537.5401263/94‑1 (MQ=60)
                                                         CGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAG                                     <  SRR4302537.3075963/94‑1 (MQ=60)
                                                         CGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAG                                     <  SRR4302537.5595941/94‑1 (MQ=60)
                                                          GAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGG                                    <  SRR4302537.2702850/94‑1 (MQ=60)
                                                          GAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGG                                    <  SRR4302537.4585369/94‑1 (MQ=60)
                                                            GCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTT                                  <  SRR4302537.6265624/94‑1 (MQ=60)
                                                             CGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTT                                 <  SRR4302537.3148847/94‑1 (MQ=60)
                                                             CGCTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTT                                 >  SRR4302537.5069504/1‑94 (MQ=60)
                                                             CGCGTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTT                                 <  SRR4302537.1820647/94‑1 (MQ=60)
                                                               CTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTC                               <  SRR4302537.6308266/94‑1 (MQ=60)
                                                               CTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTC                               <  SRR4302537.425386/94‑1 (MQ=60)
                                                               CTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTC                               <  SRR4302537.2731177/94‑1 (MQ=60)
                                                               CTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTC                               >  SRR4302537.887413/1‑94 (MQ=60)
                                                               CTTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTC                               <  SRR4302537.2523950/94‑1 (MQ=60)
                                                                TTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCA                              >  SRR4302537.3638234/1‑94 (MQ=60)
                                                                TTCGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCA                              >  SRR4302537.4347774/1‑94 (MQ=60)
                                                                  CGCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAAT                            <  SRR4302537.3405317/94‑1 (MQ=60)
                                                                   GCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATT                           <  SRR4302537.565014/94‑1 (MQ=60)
                                                                   GCTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATT                           <  SRR4302537.1978697/94‑1 (MQ=60)
                                                                    CTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTA                          >  SRR4302537.634694/1‑94 (MQ=60)
                                                                    CTCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTA                          <  SRR4302537.800996/94‑1 (MQ=60)
                                                                     TCTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTAC                         <  SRR4302537.1994596/94‑1 (MQ=60)
                                                                      CTGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACC                        <  SRR4302537.5915155/94‑1 (MQ=60)
                                                                       TGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCG                       <  SRR4302537.6169308/94‑1 (MQ=60)
                                                                       TGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCG                       <  SRR4302537.5452103/94‑1 (MQ=60)
                                                                       TGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTCTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCG                       <  SRR4302537.4585116/94‑1 (MQ=60)
                                                                       CGGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCG                       <  SRR4302537.914468/94‑1 (MQ=60)
                                                                        GGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGT                      <  SRR4302537.846518/94‑1 (MQ=60)
                                                                        GGGCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGT                      >  SRR4302537.888352/1‑94 (MQ=60)
                                                                          GCGCGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTAAATTACCGTCA                    <  SRR4302537.1866310/94‑1 (MQ=60)
                                                                             CGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTT                 >  SRR4302537.3858505/1‑94 (MQ=60)
                                                                             CGACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTT                 <  SRR4302537.4663488/94‑1 (MQ=60)
                                                                              GACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTC                <  SRR4302537.5707671/94‑1 (MQ=60)
                                                                               ACGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCA               <  SRR4302537.4241378/94‑1 (MQ=60)
                                                                                CGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCAC              <  SRR4302537.546594/94‑1 (MQ=60)
                                                                                CGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCAC              >  SRR4302537.370374/1‑94 (MQ=60)
                                                                                CGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCAC              >  SRR4302537.5695454/1‑94 (MQ=60)
                                                                                CGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCAC              >  SRR4302537.5000545/1‑94 (MQ=60)
                                                                                CGGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGGTGCTGATGGTGCGCCACCAGCAGGATTTCAATTACCGTCATTTCAC              >  SRR4302537.489716/1‑94 (MQ=60)
                                                                                 GGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACG             <  SRR4302537.5936681/94‑1 (MQ=60)
                                                                                 GGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACG             <  SRR4302537.3551851/94‑1 (MQ=60)
                                                                                 GGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACG             <  SRR4302537.3342508/94‑1 (MQ=60)
                                                                                 GGAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACG             <  SRR4302537.4201384/94‑1 (MQ=60)
                                                                                  GAAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGG            <  SRR4302537.3661144/94‑1 (MQ=60)
                                                                                   AAGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGA           >  SRR4302537.4754443/1‑94 (MQ=60)
                                                                                    AGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAA          <  SRR4302537.1529692/94‑1 (MQ=60)
                                                                                    AGGATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAA          >  SRR4302537.1443753/1‑94 (MQ=60)
                                                                                      GATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACA        <  SRR4302537.1771026/94‑1 (MQ=60)
                                                                                      GATGGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACA        <  SRR4302537.143784/94‑1 (MQ=60)
                                                                                         GGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCTCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGAC     <  SRR4302537.4253828/94‑1 (MQ=60)
                                                                                         GGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGAC     >  SRR4302537.5211283/1‑94 (MQ=60)
                                                                                         GGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGAC     <  SRR4302537.336233/94‑1 (MQ=60)
                                                                                         GGCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGAC     >  SRR4302537.4087502/1‑94 (MQ=60)
                                                                                          GCGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACG    <  SRR4302537.518691/94‑1 (MQ=60)
                                                                                           CGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGC   >  SRR4302537.2649255/1‑94 (MQ=60)
                                                                                           CGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGC   >  SRR4302537.1624261/1‑94 (MQ=60)
                                                                                           CGCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGC   >  SRR4302537.1319903/1‑94 (MQ=60)
                                                                                            GCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCA  >  SRR4302537.1841602/1‑94 (MQ=60)
                                                                                            GCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCA  >  SRR4302537.4916348/1‑94 (MQ=60)
                                                                                            GCTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCA  >  SRR4302537.4360852/1‑94 (MQ=60)
                                                                                             |                                                                                            
CCTGACGGTTGGCGTGTTCCAGCAAACCAATGCGCTCAAGCCAGGTCGCGGCGCGGTCGAGCGCTTCGCTCTGGGCGCGACGGAAGGATGGCGTTTTCAACAGGCCAGAGAACAGCCCGGTTTTCAGTTGCTGATGCTGCGCCACCAGCAGGTTTTCAATTACCGTCATTTCACGGAACAGACGCA  >  NC_000913/3591387‑3591572

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑