Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,627,142 +G intergenic (‑24/+23) yhiH ← / ← yhiI putative ATP‑binding component of a transport system, fragment 1/putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,627,1401.G99.7% 2087.7 / ‑2.7 610intergenic (‑22/+25)yhiH/yhiIputative ATP‑binding component of a transport system, fragment 1/putative membrane protein
Reads supporting (aligned to +/- strand):  ref base . (1/1);  new base G (335/272);  total (337/273)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.84e-01

Only 100 of 682 total aligned reads displayed.

GACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAC  >  NC_000913/3627047‑3627228
                                                                                              |                                                                                        
 aCCATGCAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGGTGCGCGACAGGC‑g                                                                                         >  1:8190996/1‑94 (MQ=255)
 aCCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑g                                                                                         >  1:10293209/1‑94 (MQ=255)
   cATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggg                                                                                       >  2:2485968/1‑93 (MQ=255)
    aTACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑ggga                                                                                      <  2:964371/94‑3 (MQ=255)
      aCAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gggacg                                                                                    >  2:1122468/1‑90 (MQ=255)
      aCAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gggacg                                                                                    >  1:8345766/1‑90 (MQ=255)
      aCAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑gggacg                                                                                    <  1:3631641/94‑5 (MQ=255)
        aGCGGGCCGGACTATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACggg                                                                                   <  2:9264741/94‑1 (MQ=255)
         gCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGcgggacggga                                                                                  <  2:4540954/94‑1 (MQ=255)
          cGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGaa                                                                                 >  2:10618450/1‑94 (MQ=255)
          cGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCAAGCCCGCCAGTTGCGCGACAGGCGGGACGGGaa                                                                                 >  1:6137736/1‑94 (MQ=255)
           gggCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAAc                                                                                >  1:1696556/1‑94 (MQ=255)
              ccGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAg                                                                             >  2:3957719/1‑94 (MQ=255)
              ccGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAg                                                                             >  2:7190043/1‑94 (MQ=255)
              ccGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAg                                                                             <  1:1001483/94‑1 (MQ=255)
               cGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGt                                                                            >  1:5540705/1‑94 (MQ=255)
                ggAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                           >  2:2695239/1‑94 (MQ=255)
                ggAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGtt                                                                           >  2:83953/1‑94 (MQ=255)
                  aaTATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGGACCAGTTcc                                                                         >  1:7646078/1‑94 (MQ=255)
                   atatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCa                                                                        >  1:1551330/1‑94 (MQ=255)
                   atatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCa                                                                        >  2:10223737/1‑94 (MQ=255)
                    tatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAg                                                                       >  1:4075223/1‑94 (MQ=255)
                      tCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAt                                                                     <  2:7767728/94‑1 (MQ=255)
                       cGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATg                                                                    <  1:6428290/94‑1 (MQ=255)
                        gagaGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGc                                                                   >  2:227867/1‑94 (MQ=255)
                        gagaGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGc                                                                   <  1:10122927/94‑1 (MQ=255)
                        gagaGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGc                                                                   <  2:818061/94‑1 (MQ=255)
                           agTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTc                                                                <  1:3306548/94‑1 (MQ=255)
                           agTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTc                                                                >  2:6681289/1‑94 (MQ=255)
                           agTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTCGCGCGACAGGCGGGACGGGAAACAGTTCCAGATGCgca                                                                >  1:5182974/1‑92 (MQ=255)
                            gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCa                                                               <  1:7488151/94‑1 (MQ=255)
                            gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCa                                                               <  1:4781507/94‑1 (MQ=255)
                             tGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAt                                                              >  2:1334130/1‑94 (MQ=255)
                             tGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAt                                                              <  2:7916745/94‑1 (MQ=255)
                              gATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAtt                                                             <  2:4119173/94‑1 (MQ=255)
                              gATATTGTTCAGCGAAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAtt                                                             >  2:7030498/1‑94 (MQ=255)
                               atatTGTTCAGCGCAACGTTTTTTCCATAATGCTGGCTCCCGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTg                                                            <  1:6590154/94‑1 (MQ=255)
                               atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTg                                                            >  2:3862587/1‑94 (MQ=255)
                               atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTg                                                            >  2:3450852/1‑94 (MQ=255)
                                tatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGc                                                           <  2:6375967/94‑1 (MQ=255)
                                   tGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGc                                                        >  2:5012790/1‑94 (MQ=255)
                                     ttCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCaa                                                      <  1:2745642/94‑1 (MQ=255)
                                         gcgcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTc                                                  <  1:2569468/94‑1 (MQ=255)
                                          cgcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcc                                                 >  1:2952175/1‑93 (MQ=255)
                                          cgcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTca                                                 >  1:9254293/1‑94 (MQ=255)
                                            cAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacc                                               >  1:9803726/1‑94 (MQ=255)
                                            cAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacc                                               >  1:5938893/1‑94 (MQ=255)
                                             ctcGGTTTTTCCATCATGCTGGCTAGCGCCCGCCGGTTGCGAGACAGGCGGGACGGGAACCAGTTCCAGCTGCGTCATTGCGGCGGCCTcaccc                                              <  2:9371954/92‑2 (MQ=255)
                                             aaCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                              <  2:684707/94‑1 (MQ=255)
                                             aaCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                              >  2:428291/1‑94 (MQ=255)
                                             aaCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGCTCCAGATGCGTCATTGCGGCAACCTcaccc                                              >  2:10449454/1‑93 (MQ=255)
                                               cGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACg                                            >  2:7418968/1‑94 (MQ=255)
                                               cGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACg                                            <  2:8787144/94‑1 (MQ=255)
                                                ggTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGa                                           >  2:604999/1‑94 (MQ=255)
                                                ggTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGa                                           >  2:8924902/1‑94 (MQ=255)
                                                  tttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAgg                                         <  2:2945762/94‑1 (MQ=255)
                                                   ttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAggc                                        >  1:8009130/1‑93 (MQ=255)
                                                    tttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCGCGAGgtc                                       <  2:5221298/94‑1 (MQ=255)
                                                    tttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtc                                       <  2:4845303/94‑1 (MQ=255)
                                                    tttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtc                                       >  2:1666304/1‑94 (MQ=255)
                                                    tttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtc                                       <  1:3454626/94‑1 (MQ=255)
                                                      tCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGGGGCAACCTCACCCCGAGgtcgt                                     >  2:5736463/1‑94 (MQ=255)
                                                      tCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgt                                     <  2:7302102/94‑1 (MQ=255)
                                                       ccATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgtc                                    >  1:7201775/1‑94 (MQ=255)
                                                       ccATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgtc                                    <  1:6866159/94‑1 (MQ=255)
                                                         aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGGACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                  >  2:5558867/1‑94 (MQ=255)
                                                         aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                  >  1:6639667/1‑94 (MQ=255)
                                                         aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                  >  2:6164523/1‑94 (MQ=255)
                                                         aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                  >  2:123266/1‑94 (MQ=255)
                                                         aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                  <  2:3711675/94‑1 (MQ=255)
                                                         aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                  <  2:360347/94‑1 (MQ=255)
                                                         aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                  <  2:8500317/94‑1 (MQ=255)
                                                          tAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAgg                                 <  1:9996653/94‑1 (MQ=255)
                                                          tAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAgg                                 >  2:6581734/1‑94 (MQ=255)
                                                          tAATGCTGCCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAgg                                 <  1:6253122/94‑1 (MQ=255)
                                                              gCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCAc                             >  2:3194962/1‑94 (MQ=255)
                                                              gCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCAc                             <  2:9111301/94‑1 (MQ=255)
                                                               cTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACg                            >  1:2125737/1‑94 (MQ=255)
                                                               cTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACg                            >  1:1289040/1‑94 (MQ=255)
                                                               cTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGACGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACg                            <  2:2849866/94‑1 (MQ=255)
                                                                   cTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                        >  2:5836845/1‑94 (MQ=255)
                                                                   cTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                        >  1:5281659/1‑94 (MQ=255)
                                                                   cTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                        <  2:2144423/94‑1 (MQ=255)
                                                                     cACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGtt                      <  1:10512459/94‑1 (MQ=255)
                                                                      aCGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttt                     >  1:3167994/1‑93 (MQ=255)
                                                                      aCGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttc                     >  2:3275467/1‑94 (MQ=255)
                                                                         cccgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttcttc                  >  1:4564383/1‑94 (MQ=255)
                                                                          ccgccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttca                 <  1:5741327/94‑1 (MQ=255)
                                                                            gccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGGCAGGCCACGGAAGTTCttcatt               >  2:5961757/1‑94 (MQ=255)
                                                                            gccAGTTGCGCGACAGGCGGGACGGGAACCAGGTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGGCACGGGAGTTGttcatt               >  1:3901764/1‑94 (MQ=255)
                                                                             ccAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattc              >  1:2324367/1‑94 (MQ=255)
                                                                              cAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattca             >  1:4375084/1‑94 (MQ=255)
                                                                               aGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc            <  1:1951540/94‑1 (MQ=255)
                                                                                 ttGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAccc          <  1:7791280/94‑1 (MQ=255)
                                                                                 ttGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGACACGGAAGTTCTTCATTCAccc          <  2:1873252/94‑1 (MQ=255)
                                                                                  tGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCg         <  2:3038862/94‑1 (MQ=255)
                                                                                     gcgACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAc      <  1:9095264/94‑1 (MQ=255)
                                                                                       gACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAccc    <  1:8615100/94‑1 (MQ=255)
                                                                                       gACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAccc    >  1:8835085/1‑94 (MQ=255)
                                                                                        aCAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCa   <  1:9585195/94‑1 (MQ=255)
                                                                                              |                                                                                        
GACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAC  >  NC_000913/3627047‑3627228

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 421 total aligned reads displayed.
TTAATATTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTACTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATATTCT  >  NC_000913/3652536‑3652722
                                                                                             |                                                                                             
TTAATATTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTG                                                                                               <  SRR4302545.6697724/94‑1 (MQ=60)
 TAATATTAAACGCTGCCTGGGGAAAGTGCCGGTGCCCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGC                                                                                              <  SRR4302545.9038284/94‑1 (MQ=60)
 TAATATTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGC                                                                                              >  SRR4302545.1586888/1‑94 (MQ=60)
 TAATATTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGC                                                                                              >  SRR4302545.3258687/1‑94 (MQ=60)
  AATATTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCT                                                                                             >  SRR4302545.4929795/1‑94 (MQ=60)
  AATATTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCT                                                                                             <  SRR4302545.4745665/94‑1 (MQ=60)
   ATATTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTG                                                                                            >  SRR4302545.967022/1‑94 (MQ=60)
   ATATTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTG                                                                                            >  SRR4302545.6155380/1‑94 (MQ=60)
     ATTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCT                                                                                          <  SRR4302545.3536212/94‑1 (MQ=60)
      TTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTC                                                                                         >  SRR4302545.8940165/1‑94 (MQ=60)
      TTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTC                                                                                         <  SRR4302545.9581625/94‑1 (MQ=60)
      TTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTC                                                                                         >  SRR4302545.5065015/1‑94 (MQ=60)
       TAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCT                                                                                        <  SRR4302545.8790872/94‑1 (MQ=60)
        AAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTG                                                                                       >  SRR4302545.2061207/1‑94 (MQ=60)
        AAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTG                                                                                       <  SRR4302545.1810854/94‑1 (MQ=60)
        AAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTG                                                                                       >  SRR4302545.7592885/1‑94 (MQ=60)
         AACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGT                                                                                      >  SRR4302545.10392141/1‑94 (MQ=60)
          ACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGGTTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTG                                                                                     <  SRR4302545.851089/94‑1 (MQ=60)
          ACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTG                                                                                     <  SRR4302545.4643171/94‑1 (MQ=60)
          ACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTG                                                                                     <  SRR4302545.4742492/94‑1 (MQ=60)
           CGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGC                                                                                    >  SRR4302545.8413917/1‑94 (MQ=60)
           CGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGC                                                                                    >  SRR4302545.2798089/1‑94 (MQ=60)
            GCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCC                                                                                   >  SRR4302545.1434255/1‑94 (MQ=60)
            GCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCC                                                                                   >  SRR4302545.5139836/1‑94 (MQ=60)
             CTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCT                                                                                  >  SRR4302545.8697623/1‑94 (MQ=60)
             CTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCT                                                                                  >  SRR4302545.10359841/1‑94 (MQ=60)
             CTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCT                                                                                  >  SRR4302545.7191577/1‑94 (MQ=60)
              TGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTG                                                                                 >  SRR4302545.7595044/1‑94 (MQ=60)
              TGCCTGGGGAAAGTGCCGGTGACCTTTGTTACGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTG                                                                                 >  SRR4302545.7268939/1‑94 (MQ=60)
               GCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGA                                                                                >  SRR4302545.7093062/1‑94 (MQ=60)
                CCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAA                                                                               <  SRR4302545.5106073/94‑1 (MQ=60)
                CCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAA                                                                               <  SRR4302545.3575931/94‑1 (MQ=60)
                  TGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGC                                                                             >  SRR4302545.269425/1‑94 (MQ=60)
                  TGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGC                                                                             <  SRR4302545.1025356/94‑1 (MQ=60)
                  TGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGC                                                                             <  SRR4302545.5624040/94‑1 (MQ=60)
                  TGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTAGCACTATCATTGATTTTGTAGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGC                                                                             >  SRR4302545.10797370/1‑94 (MQ=60)
                   GGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCG                                                                            <  SRR4302545.4052014/94‑1 (MQ=60)
                   GGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCG                                                                            >  SRR4302545.578805/1‑94 (MQ=60)
                    GGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGG                                                                           <  SRR4302545.5420075/94‑1 (MQ=60)
                     GGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGC                                                                          >  SRR4302545.5708719/1‑94 (MQ=60)
                     GGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGC                                                                          <  SRR4302545.9435593/94‑1 (MQ=60)
                     GGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGC                                                                          <  SRR4302545.4846861/94‑1 (MQ=60)
                       ctgatctCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTG                                                                        <  SRR4302545.5271777/87‑1 (MQ=60)
                       AAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTG                                                                        <  SRR4302545.7826259/94‑1 (MQ=60)
                       AAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTG                                                                        >  SRR4302545.1235583/1‑94 (MQ=60)
                        AAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGT                                                                       <  SRR4302545.1258157/94‑1 (MQ=60)
                         AGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTG                                                                      <  SRR4302545.5866559/94‑1 (MQ=60)
                         AGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTG                                                                      <  SRR4302545.8348435/94‑1 (MQ=60)
                          GTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGT                                                                     <  SRR4302545.4280801/94‑1 (MQ=60)
                          GTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGT                                                                     >  SRR4302545.396736/1‑94 (MQ=60)
                            GCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCA                                                                   >  SRR4302545.7650772/1‑94 (MQ=60)
                             CCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCAC                                                                  >  SRR4302545.5255926/1‑94 (MQ=60)
                             CCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCAC                                                                  <  SRR4302545.5207766/94‑1 (MQ=60)
                             CCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCAC                                                                  <  SRR4302545.7936621/94‑1 (MQ=60)
                              CGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACT                                                                 >  SRR4302545.5541716/1‑94 (MQ=60)
                               GGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTT                                                                >  SRR4302545.6030955/1‑94 (MQ=60)
                               GGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTT                                                                <  SRR4302545.1044985/94‑1 (MQ=60)
                                 TGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTC                                                              >  SRR4302545.2816718/1‑94 (MQ=60)
                                 TGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTC                                                              <  SRR4302545.6306998/94‑1 (MQ=60)
                                  GACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCG                                                             >  SRR4302545.1510438/1‑94 (MQ=60)
                                  GACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCG                                                             >  SRR4302545.4994222/1‑94 (MQ=60)
                                  GACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCG                                                             >  SRR4302545.3118375/1‑94 (MQ=60)
                                   ACCTTTGTTCCGCGTACTATCACGATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCCCTTTCGC                                                            <  SRR4302545.5758297/94‑1 (MQ=60)
                                    CCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCG                                                           <  SRR4302545.6541718/94‑1 (MQ=60)
                                     CTTTGTTCCGCGGACTATCACTATCATTTATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTggtgc                                                          >  SRR4302545.7441328/1‑89 (MQ=60)
                                     CTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGC                                                          >  SRR4302545.3651448/1‑94 (MQ=60)
                                        TGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAG                                                       <  SRR4302545.6962167/94‑1 (MQ=60)
                                         GTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGC                                                      <  SRR4302545.5756936/94‑1 (MQ=60)
                                         GTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGC                                                      <  SRR4302545.10448976/94‑1 (MQ=60)
                                          TTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCA                                                     >  SRR4302545.2375959/1‑94 (MQ=60)
                                           TCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAA                                                    <  SRR4302545.1658613/94‑1 (MQ=60)
                                           TCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAA                                                    <  SRR4302545.4874576/94‑1 (MQ=60)
                                            CCGCGGACTATCACTATCATTGATTTTGTTGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAAC                                                   >  SRR4302545.8884086/1‑94 (MQ=60)
                                            CCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAAC                                                   <  SRR4302545.9728033/94‑1 (MQ=60)
                                            CCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAAC                                                   >  SRR4302545.1701020/1‑94 (MQ=60)
                                             CGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACA                                                  >  SRR4302545.290087/1‑94 (MQ=60)
                                             CGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACA                                                  >  SRR4302545.7340727/1‑94 (MQ=60)
                                             CGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACA                                                  <  SRR4302545.1485064/94‑1 (MQ=60)
                                             CGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACA                                                  <  SRR4302545.8423149/94‑1 (MQ=60)
                                             CGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTCCTGCTCTGTGCCTGAAGCGGCTGTTTCACTTTCGCGCAAGCAACA                                                  <  SRR4302545.6360870/94‑1 (MQ=60)
                                              GCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACAT                                                 <  SRR4302545.327519/94‑1 (MQ=60)
                                               CGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATC                                                <  SRR4302545.1645935/94‑1 (MQ=60)
                                               CGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATC                                                >  SRR4302545.5427405/1‑94 (MQ=60)
                                               CGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATC                                                >  SRR4302545.2629876/1‑94 (MQ=60)
                                               CGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATC                                                <  SRR4302545.7805827/94‑1 (MQ=60)
                                               CGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATC                                                >  SRR4302545.38720/1‑94 (MQ=60)
                                               CGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAAAATC                                                >  SRR4302545.7540993/1‑94 (MQ=60)
                                                GGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTTTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCA                                               <  SRR4302545.10612008/94‑1 (MQ=60)
                                                GGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCA                                               >  SRR4302545.9255394/1‑94 (MQ=60)
                                                GGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCA                                               <  SRR4302545.557378/94‑1 (MQ=60)
                                                CGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTATGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCA                                               <  SRR4302545.4665599/94‑1 (MQ=60)
                                                 GACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAG                                              >  SRR4302545.10706127/1‑94 (MQ=60)
                                                 GACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAG                                              >  SRR4302545.585352/1‑94 (MQ=60)
                                                 GACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAG                                              <  SRR4302545.5875950/94‑1 (MQ=60)
                                                  ACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGG                                             <  SRR4302545.7117105/94‑1 (MQ=60)
                                                  ACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGG                                             >  SRR4302545.3786435/1‑94 (MQ=60)
                                                   CTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGT                                            >  SRR4302545.1288241/1‑94 (MQ=60)
                                                   CTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGT                                            <  SRR4302545.7011150/94‑1 (MQ=60)
                                                   CTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGT                                            >  SRR4302545.3536638/1‑94 (MQ=60)
                                                   CTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGT                                            >  SRR4302545.2555635/1‑94 (MQ=60)
                                                    TATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTT                                           <  SRR4302545.4581765/94‑1 (MQ=60)
                                                    TATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTT                                           <  SRR4302545.2178442/94‑1 (MQ=60)
                                                    TATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTT                                           <  SRR4302545.1139930/94‑1 (MQ=60)
                                                    TATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTT                                           <  SRR4302545.3308933/94‑1 (MQ=60)
                                                     ATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTC                                          <  SRR4302545.9406285/94‑1 (MQ=60)
                                                      TCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCT                                         <  SRR4302545.549720/94‑1 (MQ=60)
                                                      TCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCT                                         <  SRR4302545.6142340/94‑1 (MQ=60)
                                                      TCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCT                                         <  SRR4302545.6846604/94‑1 (MQ=60)
                                                       CACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTG                                        >  SRR4302545.4744433/1‑94 (MQ=60)
                                                       CACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTG                                        >  SRR4302545.9209382/1‑94 (MQ=60)
                                                       CACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTG                                        >  SRR4302545.6465155/1‑94 (MQ=60)
                                                       CACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTG                                        >  SRR4302545.8585979/1‑94 (MQ=60)
                                                        ACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGA                                       >  SRR4302545.10212678/1‑94 (MQ=60)
                                                         CTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAG                                      >  SRR4302545.3820499/1‑94 (MQ=60)
                                                         CTATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAG                                      <  SRR4302545.2551127/94‑1 (MQ=60)
                                                          TATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGC                                     >  SRR4302545.1184432/1‑94 (MQ=60)
                                                          TATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGC                                     <  SRR4302545.7307124/94‑1 (MQ=60)
                                                           ATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCT                                    <  SRR4302545.5343138/94‑1 (MQ=60)
                                                           ATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCT                                    >  SRR4302545.10588141/1‑94 (MQ=60)
                                                           ATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCT                                    <  SRR4302545.10475136/94‑1 (MQ=60)
                                                           ATCATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCT                                    <  SRR4302545.193881/94‑1 (MQ=60)
                                                             CATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGC                                  >  SRR4302545.9802806/1‑94 (MQ=60)
                                                             CATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGC                                  <  SRR4302545.7408110/94‑1 (MQ=60)
                                                              ATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCA                                 <  SRR4302545.2489005/94‑1 (MQ=60)
                                                              ATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCA                                 >  SRR4302545.8824603/1‑94 (MQ=60)
                                                              ATTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCA                                 >  SRR4302545.6623871/1‑94 (MQ=60)
                                                               TTGTTTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGGGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCAT                                <  SRR4302545.8095200/94‑1 (MQ=60)
                                                               TTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCAT                                <  SRR4302545.4138783/94‑1 (MQ=60)
                                                               TTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCAT                                >  SRR4302545.8805154/1‑94 (MQ=60)
                                                               TTGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCAT                                <  SRR4302545.271333/94‑1 (MQ=60)
                                                                TGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATT                               <  SRR4302545.6084467/94‑1 (MQ=60)
                                                                TGATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATT                               >  SRR4302545.2596849/1‑94 (MQ=60)
                                                                 GATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTG                              <  SRR4302545.3445197/94‑1 (MQ=60)
                                                                  ATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGC                             <  SRR4302545.9299906/94‑1 (MQ=60)
                                                                  ATTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGC                             <  SRR4302545.1013832/94‑1 (MQ=60)
                                                                   TTTTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCG                            >  SRR4302545.4715697/1‑94 (MQ=60)
                                                                     TTGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCGGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAA                          <  SRR4302545.1111791/94‑1 (MQ=60)
                                                                      TGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCCACATCAGGTTCTGAGCTGCATTGAGAAT                         >  SRR4302545.3945638/1‑94 (MQ=60)
                                                                      TGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAAT                         <  SRR4302545.2424062/94‑1 (MQ=60)
                                                                      TGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAAT                         >  SRR4302545.3746702/1‑94 (MQ=60)
                                                                      TGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAAT                         <  SRR4302545.7906305/94‑1 (MQ=60)
                                                                      TGTCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGATCTGAGCTGCATTGCGAAT                         >  SRR4302545.9654215/1‑94 (MQ=60)
                                                                      TGGCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAAT                         <  SRR4302545.3837422/94‑1 (MQ=60)
                                                                        TCGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCA                       <  SRR4302545.8613270/94‑1 (MQ=60)
                                                                         TGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAA                      >  SRR4302545.4146510/1‑94 (MQ=60)
                                                                         TGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGGTCTGAGCTGCATTGCGAATCGA                      >  SRR4302545.1117167/1‑94 (MQ=60)
                                                                         CGCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAA                      >  SRR4302545.1896724/1‑94 (MQ=60)
                                                                          GCACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAA                     >  SRR4302545.4855426/1‑94 (MQ=60)
                                                                           CACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAAT                    >  SRR4302545.7731459/1‑94 (MQ=60)
                                                                           CACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAAT                    <  SRR4302545.79438/94‑1 (MQ=60)
                                                                           CACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAAT                    >  SRR4302545.3411721/1‑94 (MQ=60)
                                                                           CACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAAT                    >  SRR4302545.315088/1‑94 (MQ=60)
                                                                           CACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAAT                    <  SRR4302545.6549005/94‑1 (MQ=60)
                                                                           CACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAAT                    >  SRR4302545.9868449/1‑94 (MQ=60)
                                                                           CACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAAT                    <  SRR4302545.4962987/94‑1 (MQ=60)
                                                                            ACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATG                   <  SRR4302545.8467/94‑1 (MQ=60)
                                                                            ACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATG                   <  SRR4302545.3501673/94‑1 (MQ=60)
                                                                            ACTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATG                   >  SRR4302545.8946786/1‑94 (MQ=60)
                                                                             CTCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGA                  <  SRR4302545.7429926/94‑1 (MQ=60)
                                                                              TCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGAC                 <  SRR4302545.103473/94‑1 (MQ=60)
                                                                              TCATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGAC                 <  SRR4302545.9746008/94‑1 (MQ=60)
                                                                               CATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACA                <  SRR4302545.8809850/94‑1 (MQ=60)
                                                                                ATCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAA               >  SRR4302545.10070392/1‑94 (MQ=60)
                                                                                 TCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAAC              <  SRR4302545.1061441/94‑1 (MQ=60)
                                                                                 TCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAAC              <  SRR4302545.2805016/94‑1 (MQ=60)
                                                                                 TCAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAAC              <  SRR4302545.6018809/94‑1 (MQ=60)
                                                                                  CAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACG             >  SRR4302545.10529505/1‑94 (MQ=60)
                                                                                  CAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACG             >  SRR4302545.7745220/1‑94 (MQ=60)
                                                                                  CAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACG             <  SRR4302545.1729153/94‑1 (MQ=60)
                                                                                  CAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACG             <  SRR4302545.298907/94‑1 (MQ=60)
                                                                                  CAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACG             >  SRR4302545.3020809/1‑94 (MQ=60)
                                                                                  CAATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGCGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACG             >  SRR4302545.6865506/1‑94 (MQ=60)
                                                                                   AATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGG            >  SRR4302545.6905736/1‑94 (MQ=60)
                                                                                   AATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGG            >  SRR4302545.6227991/1‑94 (MQ=60)
                                                                                   AATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGG            >  SRR4302545.10731938/1‑94 (MQ=60)
                                                                                   AATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGG            >  SRR4302545.4208501/1‑94 (MQ=60)
                                                                                    ATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCCCTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGA           <  SRR4302545.8748001/94‑1 (MQ=60)
                                                                                    ATGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGA           <  SRR4302545.3597424/94‑1 (MQ=60)
                                                                                     TGGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAA          >  SRR4302545.443941/1‑94 (MQ=60)
                                                                                     TGGCAGTTGCTGCTCTGCGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAA          <  SRR4302545.7460613/94‑1 (MQ=60)
                                                                                      GGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAG         >  SRR4302545.4273564/1‑94 (MQ=60)
                                                                                      GGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAG         <  SRR4302545.9493692/94‑1 (MQ=60)
                                                                                      GGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAG         <  SRR4302545.1551775/94‑1 (MQ=60)
                                                                                      GGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAG         <  SRR4302545.9116148/94‑1 (MQ=60)
                                                                                      GGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAG         <  SRR4302545.2201562/94‑1 (MQ=60)
                                                                                      GGCAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAG         <  SRR4302545.8175106/94‑1 (MQ=60)
                                                                                        CAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGAT       >  SRR4302545.9933823/1‑94 (MQ=60)
                                                                                        CAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGAT       <  SRR4302545.2876510/94‑1 (MQ=60)
                                                                                        CAGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGAT       <  SRR4302545.8228758/94‑1 (MQ=60)
                                                                                         AGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATA      <  SRR4302545.4333348/94‑1 (MQ=60)
                                                                                         AGTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATA      <  SRR4302545.9164587/94‑1 (MQ=60)
                                                                                          GTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATAT     >  SRR4302545.3700730/1‑94 (MQ=60)
                                                                                          GTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATAT     <  SRR4302545.5587195/94‑1 (MQ=60)
                                                                                          GTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATAT     <  SRR4302545.650618/94‑1 (MQ=60)
                                                                                          GTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATAT     >  SRR4302545.3107943/1‑94 (MQ=60)
                                                                                          GTTGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATAT     >  SRR4302545.4239815/1‑94 (MQ=60)
                                                                                            TGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATATTC   >  SRR4302545.9494165/1‑94 (MQ=60)
                                                                                            TGCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATATTC   >  SRR4302545.9776007/1‑94 (MQ=60)
                                                                                             GCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATATTCT  <  SRR4302545.6050516/94‑1 (MQ=60)
                                                                                             GCTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATATTCT  >  SRR4302545.8640112/1‑94 (MQ=60)
                                                                                             |                                                                                             
TTAATATTAAACGCTGCCTGGGGAAAGTGCCGGTGACCTTTGTTCCGCGGACTATCACTATCATTGATTTTGTCGCACTCATCAATGGCAGTTACTGCTCTGTGCCTGAAGCGGCTGTGTCACTTTCGCGCAAGCAACATCAGGTTCTGAGCTGCATTGCGAATCAAATGACAACGGAAGATATTCT  >  NC_000913/3652536‑3652722

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑