Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,675,983 T→C T5A (ACG→GCG)  yhjG ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,675,9830TC99.5% 2219.1 / ‑2.9 637T5A (ACG→GCG) yhjGhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (344/290);  total (346/291)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.42e-01

Only 100 of 676 total aligned reads displayed.

TCAAAAGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGTTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAACCGCCGTATT  >  NC_000913/3675892‑3676073
                                                                                           |                                                                                          
tCAAAAGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTc                                                                                          <  2:655734/94‑1 (MQ=255)
 cAAAAGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCg                                                                                         <  1:7108953/94‑1 (MQ=255)
 cAAAAGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCg                                                                                         <  1:8706013/94‑1 (MQ=255)
  aaaaGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCAtcgctcgc                                                                                        >  1:6460580/1‑94 (MQ=255)
     aGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTc                                                                                     <  1:10327177/94‑1 (MQ=255)
      gTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTcc                                                                                    >  1:261072/1‑94 (MQ=255)
       ttGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCt                                                                                   >  2:2012456/1‑94 (MQ=255)
       ttGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCt                                                                                   <  1:8604741/94‑1 (MQ=255)
       ttGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCt                                                                                   <  2:5827444/94‑1 (MQ=255)
       ttGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCt                                                                                   <  2:146408/94‑1 (MQ=255)
       tggCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCt                                                                                   <  1:952582/92‑1 (MQ=255)
         gCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCa                                                                                 >  2:7119200/1‑94 (MQ=255)
          cAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCat                                                                                >  2:3969451/1‑94 (MQ=255)
          cAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCat                                                                                >  1:7790063/1‑94 (MQ=255)
          cAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCat                                                                                <  2:7276633/94‑1 (MQ=255)
             tCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATAtt                                                                             >  2:5926882/1‑94 (MQ=255)
                aaaTGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATAttctt                                                                          <  2:3848317/94‑1 (MQ=255)
                 aaTGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATAttcttc                                                                         <  2:3333408/94‑1 (MQ=255)
                 aaTGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATAttcttc                                                                         <  1:2735280/94‑1 (MQ=255)
                   tGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCt                                                                       >  1:2479387/1‑94 (MQ=255)
                   tGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCt                                                                       >  1:6133339/1‑94 (MQ=255)
                    gATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCtg                                                                      >  1:3945127/1‑94 (MQ=255)
                    gATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCtg                                                                      <  2:8703595/94‑1 (MQ=255)
                    gATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCtg                                                                      >  1:8224241/1‑94 (MQ=255)
                     aTCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCtgt                                                                     <  2:3062050/94‑1 (MQ=255)
                      tCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCtgtg                                                                    >  2:2504837/1‑94 (MQ=255)
                        gCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGcc                                                                  >  2:8505899/1‑94 (MQ=255)
                         ccACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCa                                                                 <  1:5463271/94‑1 (MQ=255)
                          cACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAg                                                                <  2:5490226/94‑1 (MQ=255)
                          cACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAg                                                                >  2:162769/1‑94 (MQ=255)
                          cACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCGCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAg                                                                <  2:1819247/94‑1 (MQ=255)
                           aCGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGt                                                               <  2:6692014/94‑1 (MQ=255)
                             gACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTcc                                                             >  2:2294270/1‑94 (MQ=255)
                              aCAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCt                                                            <  2:6209362/94‑1 (MQ=255)
                                aaTCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTaa                                                          <  2:4281978/94‑1 (MQ=255)
                                 aTCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGGTCATCGCTCGCTTCCTCGTATTCTTCCTGTGTGAGTCCTaaa                                                         >  2:7506395/1‑94 (MQ=255)
                                 aTCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTaaa                                                         >  2:8928705/1‑94 (MQ=255)
                                 aTCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTaaa                                                         >  2:835556/1‑94 (MQ=255)
                                  tCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAg                                                        <  2:2093315/94‑1 (MQ=255)
                                   caacaacaaGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGa                                                       >  1:3070699/1‑94 (MQ=255)
                                    aacaacaaGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGaa                                                      >  1:6563404/1‑93 (MQ=255)
                                    aacaacaaGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGAt                                                      >  1:6844178/1‑94 (MQ=255)
                                     acaacaaGAAAGCCCCTGAAATCGCATCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATa                                                     <  2:6023008/94‑1 (MQ=255)
                                     acaacaaGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATa                                                     <  2:3696732/94‑1 (MQ=255)
                                     acaacaaGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATa                                                     <  2:2897471/94‑1 (MQ=255)
                                       aacaaGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGt                                                   >  1:6212308/1‑94 (MQ=255)
                                        acaaGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTc                                                  <  1:9383618/94‑1 (MQ=255)
                                         caaGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTcc                                                 >  1:2076730/1‑94 (MQ=255)
                                          aaGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTcca                                                <  2:7622944/94‑1 (MQ=255)
                                          aaGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTcca                                                <  1:7282879/94‑1 (MQ=255)
                                           aGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTccag                                               >  1:346914/1‑94 (MQ=255)
                                           aGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTccag                                               >  1:8057887/1‑94 (MQ=255)
                                           aGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCCGTGCCAGTCCTAAAGATAGTgcag                                               >  1:1767607/1‑94 (MQ=255)
                                            gAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTccagc                                              >  2:2686102/1‑94 (MQ=255)
                                             aaaGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTccagcc                                             <  1:5120239/94‑1 (MQ=255)
                                             aaaGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTccagcc                                             <  2:8263703/94‑1 (MQ=255)
                                             aaaGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTccagcc                                             >  2:6952527/1‑94 (MQ=255)
                                              aaGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTccagcca                                            <  2:5326125/94‑1 (MQ=255)
                                                gCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAgg                                          <  2:6328131/94‑1 (MQ=255)
                                                 ccccTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGc                                         <  1:1382879/94‑1 (MQ=255)
                                                  cccTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCg                                        >  2:7981467/1‑94 (MQ=255)
                                                    cTGAAATCGCAGCGGGTATTTTGCCTGCCTGGCTCATCGCTCGCTTCCTCATATTCTTCATGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGa                                      <  2:9694999/94‑1 (MQ=255)
                                                     tGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGaa                                     <  2:6825779/94‑1 (MQ=255)
                                                       aaaTCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGaaaa                                   >  1:7572135/1‑94 (MQ=255)
                                                          tCGCAGCTGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGa                                <  2:10220234/94‑1 (MQ=255)
                                                           cGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAg                               >  1:6931072/1‑94 (MQ=255)
                                                           cGCAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTACAGCCAGGCGGAAAATGAg                               >  1:9627295/1‑94 (MQ=255)
                                                           cGCAGCGGTTATTTTGCCTGCCTTGCTAATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTCAAGATAGTCCAGCCAGGCGGAAAATGAg                               >  2:3540028/1‑94 (MQ=255)
                                                             cAGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGc                             >  1:9212296/1‑94 (MQ=255)
                                                              aGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCa                            >  1:10128559/1‑94 (MQ=255)
                                                              aGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCa                            >  1:5710849/1‑94 (MQ=255)
                                                              aGCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCa                            <  1:7426192/94‑1 (MQ=255)
                                                               gCGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAg                           <  2:9169348/94‑1 (MQ=255)
                                                                cGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGc                          <  1:4084853/94‑1 (MQ=255)
                                                                cGGTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGc                          >  2:3176052/1‑94 (MQ=255)
                                                                  gTTATTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCtt                        >  2:939388/1‑94 (MQ=255)
                                                                     aTTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTtata                     <  1:1299865/94‑1 (MQ=255)
                                                                     aTTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTtata                     <  2:7790062/94‑1 (MQ=255)
                                                                     aTTTTGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTtata                     >  2:565974/1‑94 (MQ=255)
                                                                       tttGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAgc                   <  1:6026776/94‑1 (MQ=255)
                                                                        ttGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAgcg                  <  2:4440914/94‑1 (MQ=255)
                                                                        ttGCCTGCCTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAgcg                  <  1:10605592/94‑1 (MQ=255)
                                                                         tgcctgccTTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAgcgc                 >  1:3672600/1‑94 (MQ=255)
                                                                          gcctgcctTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTCTAGCGcc                >  1:6305875/1‑94 (MQ=255)
                                                                            ctgcctTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAg              >  1:4385097/1‑94 (MQ=255)
                                                                            ctgcctTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAg              <  2:5008094/94‑1 (MQ=255)
                                                                             tgcctTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGa             >  2:1213707/1‑94 (MQ=255)
                                                                                ctTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGaaac          >  1:9970351/1‑92 (MQ=255)
                                                                                ctTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAAcc          >  2:10045075/1‑94 (MQ=255)
                                                                                ctTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAAcc          <  2:8810844/94‑1 (MQ=255)
                                                                                ctTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAAcc          >  1:2951150/1‑94 (MQ=255)
                                                                                ctTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAAcc          <  1:3251163/94‑1 (MQ=255)
                                                                                ctTGCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCACAAcc          >  2:10597112/1‑94 (MQ=255)
                                                                                   gCTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAAccgcc       >  2:10336510/1‑94 (MQ=255)
                                                                                    cTCATCGCTCGCTTCCTCATATTCTTCCTGTGTCAGTCCTAAAGATAGTCCAGCCAGGCGGCAAATGAGGCAGCTTATAGGGCCAGAAccgtcg      >  1:10020181/1‑94 (MQ=255)
                                                                                    cTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAAccgccg      >  1:1633868/1‑94 (MQ=255)
                                                                                    cTCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAAccgccg      <  1:8916566/94‑1 (MQ=255)
                                                                                     tCATCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAACCGCCGt     <  1:8371847/94‑1 (MQ=255)
                                                                                       aTCGCTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAACCGCCGTAt   >  1:3370996/1‑94 (MQ=255)
                                                                                        tcgctcgcTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAACCGCCGTAtt  <  1:229963/94‑1 (MQ=255)
                                                                                           |                                                                                          
TCAAAAGTTGCAATCAAAATGATCGCCACGACAATCAACAACAAGAAAGCCCCTGAAATCGCAGCGGTTATTTTGCCTGCCTTGCTCATCGTTCGCTTCCTCATATTCTTCCTGTGCCAGTCCTAAAGATAGTCCAGCCAGGCGGAAAATGAGGCAGCTTATAGCGCCAGAACCGCCGTATT  >  NC_000913/3675892‑3676073

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 440 total aligned reads displayed.
GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  NC_000913/3686682‑3686868
                                                                                              |                                                                                              
GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G                                                                                                >  SRR4302545.3408280/1‑94 (MQ=60)
GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G                                                                                                <  SRR4302545.222410/94‑1 (MQ=60)
GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G                                                                                                <  SRR4302545.8031451/94‑1 (MQ=60)
GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G                                                                                                <  SRR4302545.5689680/94‑1 (MQ=60)
GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G                                                                                                <  SRR4302545.4277490/94‑1 (MQ=60)
  CGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑Gtg                                                                                              <  SRR4302545.4333443/94‑3 (MQ=60)
   GCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑Gtga                                                                                             >  SRR4302545.3710644/1‑91 (MQ=60)
    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAA                                                                                            >  SRR4302545.7465249/1‑94 (MQ=60)
    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAA                                                                                            >  SRR4302545.705388/1‑94 (MQ=60)
    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAA                                                                                            >  SRR4302545.6379473/1‑94 (MQ=60)
    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAA                                                                                            >  SRR4302545.3596364/1‑94 (MQ=60)
    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAA                                                                                            >  SRR4302545.2284178/1‑94 (MQ=60)
     TTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAAT                                                                                           >  SRR4302545.10257082/1‑94 (MQ=60)
       CGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCGGACCGAGGGAAAGC‑Gtgaatag                                                                                         >  SRR4302545.7540213/1‑87 (MQ=60)
       CGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATAT                                                                                         >  SRR4302545.6076188/1‑94 (MQ=60)
        GCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATG                                                                                        <  SRR4302545.7264922/94‑1 (MQ=60)
        GCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATG                                                                                        <  SRR4302545.9711880/94‑1 (MQ=60)
        GCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATG                                                                                        <  SRR4302545.2200111/94‑1 (MQ=60)
        GCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATG                                                                                        >  SRR4302545.10892973/1‑94 (MQ=60)
         CCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGT                                                                                       >  SRR4302545.1356760/1‑94 (MQ=60)
          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTT                                                                                      <  SRR4302545.8083981/94‑1 (MQ=60)
           TAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTT                                                                                     <  SRR4302545.10288069/94‑1 (MQ=60)
           TAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTT                                                                                     <  SRR4302545.5802032/94‑1 (MQ=60)
           TAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTT                                                                                     <  SRR4302545.2357835/94‑1 (MQ=60)
           TAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTT                                                                                     <  SRR4302545.10693017/94‑1 (MQ=60)
           TAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTT                                                                                     >  SRR4302545.4104435/1‑94 (MQ=60)
           TAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGCCCAACCCGAGGAAAAGC‑GTGATTATGTTT                                                                                     >  SRR4302545.9275087/1‑94 (MQ=60)
           TAACCGAACGTGCTCCAGCCACTTGTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTT                                                                                     <  SRR4302545.5969234/94‑1 (MQ=60)
            AACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTA                                                                                    >  SRR4302545.7750957/1‑94 (MQ=60)
             ACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGCGG‑GAATATGTTTAG                                                                                   >  SRR4302545.8392451/1‑94 (MQ=60)
             ACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAG                                                                                   >  SRR4302545.1612815/1‑94 (MQ=60)
             ACCGAACCTGCTTCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGATTAG                                                                                   >  SRR4302545.9679967/1‑94 (MQ=60)
              CCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGT                                                                                  <  SRR4302545.3300214/94‑1 (MQ=60)
              CCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGT                                                                                  <  SRR4302545.9233765/94‑1 (MQ=60)
              CCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGT                                                                                  >  SRR4302545.6249327/1‑94 (MQ=60)
              CCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGT                                                                                  <  SRR4302545.706306/94‑1 (MQ=60)
              CCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGT                                                                                  <  SRR4302545.8407805/94‑1 (MQ=60)
              CCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGT                                                                                  >  SRR4302545.10119952/1‑94 (MQ=60)
               CGACCTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTG                                                                                 <  SRR4302545.3607071/94‑1 (MQ=60)
               CGAACTTGCTCCATCAACTGTTGCTGAGCGGTTGGTGCCGCCTCGACCATCGGCATGACGTCCAGACCGAGGGATAGC‑GTGAATAGGTTTAGTG                                                                                 >  SRR4302545.9694952/1‑94 (MQ=60)
               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGCAAGC‑GTGAATATGTTTAGTG                                                                                 >  SRR4302545.2132469/1‑94 (MQ=60)
               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTG                                                                                 >  SRR4302545.9559711/1‑94 (MQ=60)
               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTG                                                                                 <  SRR4302545.9290831/94‑1 (MQ=60)
                GAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGGAAGC‑GTGAATATGTTTAGTGT                                                                                >  SRR4302545.6298389/1‑94 (MQ=60)
                GAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGT                                                                                >  SRR4302545.3827178/1‑94 (MQ=60)
                GAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGT                                                                                >  SRR4302545.6629434/1‑94 (MQ=60)
                GAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGT                                                                                >  SRR4302545.3494845/1‑94 (MQ=60)
                GAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGT                                                                                >  SRR4302545.5374860/1‑94 (MQ=60)
                GAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGT                                                                                <  SRR4302545.9832439/94‑1 (MQ=60)
                 AACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTG                                                                               >  SRR4302545.9494273/1‑94 (MQ=60)
                 AACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTG                                                                               <  SRR4302545.5068088/94‑1 (MQ=60)
                  ACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGA                                                                              <  SRR4302545.2426837/94‑1 (MQ=60)
                  ACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGA                                                                              <  SRR4302545.3483459/94‑1 (MQ=60)
                  ACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGA                                                                              <  SRR4302545.777760/94‑1 (MQ=60)
                   CTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAA                                                                             <  SRR4302545.8704988/94‑1 (MQ=60)
                     TGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATT                                                                           >  SRR4302545.8232966/1‑94 (MQ=60)
                     TGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATT                                                                           >  SRR4302545.3188996/1‑94 (MQ=60)
                       CTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGCCCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTG                                                                         <  SRR4302545.294672/94‑1 (MQ=60)
                       CTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTG                                                                         >  SRR4302545.7444323/1‑94 (MQ=60)
                       CTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTG                                                                         <  SRR4302545.8074920/94‑1 (MQ=60)
                        TCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGC                                                                        <  SRR4302545.10612152/94‑1 (MQ=60)
                        TCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGC                                                                        <  SRR4302545.9397903/94‑1 (MQ=60)
                          CAGCAGCTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGC                                                                      <  SRR4302545.2213670/94‑1 (MQ=60)
                          CAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGACAGC‑GTGAGTAGGTTTAGTGCGAATTTGCGC                                                                      >  SRR4302545.6786371/1‑94 (MQ=60)
                          CAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGC                                                                      >  SRR4302545.3517066/1‑94 (MQ=60)
                           AGCACCTGTGGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCA                                                                     <  SRR4302545.4297905/94‑1 (MQ=60)
                           AGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCA                                                                     >  SRR4302545.4163790/1‑94 (MQ=60)
                            GCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCAT                                                                    >  SRR4302545.8751005/1‑94 (MQ=60)
                            GCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCAT                                                                    <  SRR4302545.9133556/94‑1 (MQ=60)
                             CAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATT                                                                   <  SRR4302545.1653134/94‑1 (MQ=60)
                              AACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTC                                                                  >  SRR4302545.7480729/1‑94 (MQ=60)
                              AACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTC                                                                  >  SRR4302545.6488892/1‑94 (MQ=60)
                              AACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTC                                                                  <  SRR4302545.882284/94‑1 (MQ=60)
                               ACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCC                                                                 <  SRR4302545.4535877/94‑1 (MQ=60)
                               ACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCC                                                                 >  SRR4302545.1847654/1‑94 (MQ=60)
                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302545.4851503/94‑1 (MQ=60)
                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302545.2006915/94‑1 (MQ=60)
                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302545.887025/94‑1 (MQ=60)
                                 TGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTG                                                               <  SRR4302545.9046272/94‑1 (MQ=60)
                                  GTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGG                                                              >  SRR4302545.6670755/1‑94 (MQ=60)
                                  GTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGG                                                              <  SRR4302545.6317061/94‑1 (MQ=60)
                                  GTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGG                                                              <  SRR4302545.10275359/94‑1 (MQ=60)
                                  GTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGG                                                              >  SRR4302545.5614401/1‑94 (MQ=60)
                                  GTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCACACCGAGGGAAAGC‑GCGAATATGTTTAGTGTGAATTGGCGCATTTCTGG                                                              >  SRR4302545.10046637/1‑94 (MQ=60)
                                   TTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGC                                                             >  SRR4302545.8484650/1‑94 (MQ=60)
                                      CTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCC                                                          >  SRR4302545.6820845/1‑94 (MQ=60)
                                      CTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCC                                                          >  SRR4302545.9728317/1‑94 (MQ=60)
                                       TGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCA                                                         <  SRR4302545.3640252/94‑1 (MQ=60)
                                         AGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGT                                                       >  SRR4302545.5235807/1‑94 (MQ=60)
                                         AGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGT                                                       >  SRR4302545.3414396/1‑94 (MQ=60)
                                         AGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGT                                                       <  SRR4302545.5732470/94‑1 (MQ=60)
                                          GCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTC                                                      <  SRR4302545.9772041/94‑1 (MQ=60)
                                           CGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCA                                                     <  SRR4302545.1273944/94‑1 (MQ=60)
                                           CGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCA                                                     <  SRR4302545.1009311/94‑1 (MQ=60)
                                           CGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCA                                                     >  SRR4302545.5131768/1‑94 (MQ=60)
                                           CGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCA                                                     <  SRR4302545.1051858/94‑1 (MQ=60)
                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    >  SRR4302545.4034511/1‑94 (MQ=60)
                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    <  SRR4302545.10793536/94‑1 (MQ=60)
                                             GTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGG                                                   <  SRR4302545.6594134/94‑1 (MQ=60)
                                             GTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGG                                                   <  SRR4302545.7148063/94‑1 (MQ=60)
                                             GTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGG                                                   <  SRR4302545.5790889/94‑1 (MQ=60)
                                             GTGGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGG                                                   <  SRR4302545.3129451/94‑1 (MQ=60)
                                              TTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGA                                                  >  SRR4302545.1739332/1‑94 (MQ=60)
                                               TGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTA                                                 <  SRR4302545.6721716/94‑1 (MQ=60)
                                               TGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTA                                                 <  SRR4302545.2121529/94‑1 (MQ=60)
                                               TGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTAAGGTA                                                 >  SRR4302545.9430602/1‑94 (MQ=60)
                                                GGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAA                                                <  SRR4302545.271520/94‑1 (MQ=60)
                                                GGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAA                                                <  SRR4302545.8436562/94‑1 (MQ=60)
                                                GGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAA                                                >  SRR4302545.1335202/1‑94 (MQ=60)
                                                GGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAA                                                >  SRR4302545.7874014/1‑94 (MQ=60)
                                                 GTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAAT                                               <  SRR4302545.7655954/94‑1 (MQ=60)
                                                    CTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAC                                            <  SRR4302545.3078003/94‑1 (MQ=60)
                                                    CTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAC                                            >  SRR4302545.8550149/1‑94 (MQ=60)
                                                     TGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACT                                           >  SRR4302545.9573606/1‑94 (MQ=60)
                                                     TGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACT                                           >  SRR4302545.9870076/1‑94 (MQ=60)
                                                      GCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTC                                          <  SRR4302545.6745512/94‑1 (MQ=60)
                                                      GCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGGACTAACTC                                          >  SRR4302545.703051/1‑94 (MQ=60)
                                                       CCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCA                                         <  SRR4302545.1885802/94‑1 (MQ=60)
                                                       CCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCA                                         <  SRR4302545.5535068/94‑1 (MQ=60)
                                                       CCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCACTCCTGGCCCCAGTCAGGTAATAACTCA                                         >  SRR4302545.8004550/1‑94 (MQ=60)
                                                        CTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAC                                        >  SRR4302545.10489617/1‑94 (MQ=60)
                                                         TCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACC                                       >  SRR4302545.7923432/1‑94 (MQ=60)
                                                          CGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCT                                      >  SRR4302545.4203051/1‑94 (MQ=60)
                                                            ACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATATCTCACCTTT                                    <  SRR4302545.3920433/94‑1 (MQ=60)
                                                            ACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTT                                    <  SRR4302545.10417875/94‑1 (MQ=60)
                                                            ACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTT                                    >  SRR4302545.5438392/1‑94 (MQ=60)
                                                              CATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTG                                  <  SRR4302545.1508233/94‑1 (MQ=60)
                                                               ATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGT                                 >  SRR4302545.1087719/1‑94 (MQ=60)
                                                               ATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGT                                 >  SRR4302545.3945826/1‑94 (MQ=60)
                                                               ATCGACATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGT                                 >  SRR4302545.5901127/1‑94 (MQ=60)
                                                                TCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTTGCCCCAGTCAGGTAATAACTCACCTTTTGTC                                >  SRR4302545.10641780/1‑94 (MQ=60)
                                                                TCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCATGTAATAACTCACCTTTTGTC                                <  SRR4302545.1197089/94‑1 (MQ=60)
                                                                TCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTC                                <  SRR4302545.8464956/94‑1 (MQ=60)
                                                                TCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTC                                <  SRR4302545.648594/94‑1 (MQ=60)
                                                                 CGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGTGCCAGTCAGGTAATAACTCGGCTTTTCTCG                               >  SRR4302545.6453956/1‑94 (MQ=60)
                                                                 CGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCG                               <  SRR4302545.9011375/94‑1 (MQ=60)
                                                                 CGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCG                               <  SRR4302545.1245226/94‑1 (MQ=60)
                                                                 CGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCA                               <  SRR4302545.101874/94‑1 (MQ=60)
                                                                  GGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGA                              >  SRR4302545.992269/1‑94 (MQ=60)
                                                                  GGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGA                              <  SRR4302545.636864/94‑1 (MQ=60)
                                                                  GGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGA                              <  SRR4302545.1096060/94‑1 (MQ=60)
                                                                    CATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTCGCCCCAGTCAGGCAATAACTCACCTTTTGTCGAGA                            >  SRR4302545.7351071/1‑94 (MQ=60)
                                                                     ATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA                           >  SRR4302545.9645174/1‑94 (MQ=60)
                                                                     ATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA                           >  SRR4302545.281953/1‑94 (MQ=60)
                                                                     ATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAA                           <  SRR4302545.7860860/94‑1 (MQ=60)
                                                                      TGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAG                          <  SRR4302545.6917069/94‑1 (MQ=60)
                                                                      TGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAG                          <  SRR4302545.6246382/94‑1 (MQ=60)
                                                                      TGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAG                          <  SRR4302545.8648156/94‑1 (MQ=60)
                                                                      TGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAG                          >  SRR4302545.5864973/1‑94 (MQ=60)
                                                                      TGACGGACAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAG                          <  SRR4302545.5741453/94‑1 (MQ=60)
                                                                        ACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCG                        >  SRR4302545.720358/1‑94 (MQ=60)
                                                                         CGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGG                       <  SRR4302545.2605752/94‑1 (MQ=60)
                                                                         CGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGG                       <  SRR4302545.6206122/94‑1 (MQ=60)
                                                                         CGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGG                       <  SRR4302545.7318757/94‑1 (MQ=60)
                                                                          GGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGA                      <  SRR4302545.10546859/94‑1 (MQ=60)
                                                                           GCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAA                     >  SRR4302545.5130284/1‑94 (MQ=60)
                                                                           GCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAA                     <  SRR4302545.905532/94‑1 (MQ=60)
                                                                            CCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAA                    <  SRR4302545.1498822/94‑1 (MQ=60)
                                                                            CCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAA                    <  SRR4302545.4080408/94‑1 (MQ=60)
                                                                             CAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAC                   <  SRR4302545.9265098/94‑1 (MQ=60)
                                                                             CAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAC                   <  SRR4302545.3350697/94‑1 (MQ=60)
                                                                             CAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAC                   <  SRR4302545.8878784/94‑1 (MQ=60)
                                                                              AGACCGAGGTAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACG                  <  SRR4302545.1034312/94‑1 (MQ=60)
                                                                                ACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGT                >  SRR4302545.4690721/1‑94 (MQ=60)
                                                                                ACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGT                >  SRR4302545.9460994/1‑94 (MQ=60)
                                                                                ACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGT                >  SRR4302545.5929286/1‑94 (MQ=60)
                                                                                  TGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              >  SRR4302545.4222478/1‑94 (MQ=60)
                                                                                  CGAGGGAAAGCTT‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCACTCAGGTAATAACTCACCTTTTGTCGATACGCGGAAACGGTGT              >  SRR4302545.5303412/1‑94 (MQ=60)
                                                                                  CGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              >  SRR4302545.4748438/1‑94 (MQ=60)
                                                                                  CGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              >  SRR4302545.198520/1‑94 (MQ=60)
                                                                                  CGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              >  SRR4302545.6175993/1‑94 (MQ=60)
                                                                                  CGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              >  SRR4302545.3531668/1‑94 (MQ=60)
                                                                                   GAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTT             <  SRR4302545.7330279/94‑1 (MQ=60)
                                                                                   GAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTT             >  SRR4302545.10566731/1‑94 (MQ=60)
                                                                                   GAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTT             >  SRR4302545.557783/1‑94 (MQ=60)
                                                                                   GAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTT             <  SRR4302545.7176942/94‑1 (MQ=60)
                                                                                    AGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTTTCGAGAAGCGGAAACGGTGTTG            >  SRR4302545.8914226/1‑94 (MQ=60)
                                                                                    AGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTG            <  SRR4302545.8298602/94‑1 (MQ=60)
                                                                                    AGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTG            <  SRR4302545.4928784/94‑1 (MQ=60)
                                                                                    AGGGAAAGC‑GTCAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTG            <  SRR4302545.5411702/94‑1 (MQ=60)
                                                                                     GGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGA           <  SRR4302545.10016364/94‑1 (MQ=60)
                                                                                     GGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGA           <  SRR4302545.8255391/94‑1 (MQ=60)
                                                                                     GGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGA           <  SRR4302545.2502087/94‑1 (MQ=60)
                                                                                      GGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAT          >  SRR4302545.10640068/1‑94 (MQ=60)
                                                                                      GGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAT          <  SRR4302545.756097/94‑1 (MQ=60)
                                                                                      GGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAT          >  SRR4302545.9912102/1‑94 (MQ=60)
                                                                                       GAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC         <  SRR4302545.35501/94‑1 (MQ=60)
                                                                                        AAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCC        <  SRR4302545.2632280/94‑1 (MQ=60)
                                                                                        AAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCC        >  SRR4302545.443709/1‑94 (MQ=60)
                                                                                         AAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCC       >  SRR4302545.4619281/1‑94 (MQ=60)
                                                                                         AAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCC       <  SRR4302545.8975273/94‑1 (MQ=60)
                                                                                          AGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      >  SRR4302545.10004875/1‑94 (MQ=60)
                                                                                          AGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      <  SRR4302545.1145112/94‑1 (MQ=60)
                                                                                          AGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      >  SRR4302545.1401192/1‑94 (MQ=60)
                                                                                          AGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      <  SRR4302545.2757876/94‑1 (MQ=60)
                                                                                          AGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      >  SRR4302545.8606229/1‑94 (MQ=60)
                                                                                           GC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAG     <  SRR4302545.7699648/94‑1 (MQ=60)
                                                                                              T‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  SRR4302545.7613166/1‑94 (MQ=60)
                                                                                              T‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  SRR4302545.1693856/1‑94 (MQ=60)
                                                                                              T‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  SRR4302545.8865131/1‑94 (MQ=60)
                                                                                              |                                                                                              
GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  NC_000913/3686682‑3686868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑