Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,686,775	+T	intergenic (‑23/+9)	b3530 ← / ← yhjM	putative oxidoreductase subunit/putative endoglucanase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,686,775	1	.	T	99.6%	873.3 / ‑5.3	246	intergenic (‑23/+9)	b3530/yhjM	putative oxidoreductase subunit/putative endoglucanase
Reads supporting (aligned to +/- strand): ref base . (1/0); major base T (110/134); minor base A (1/0); total (112/134)
Fisher's exact test for biased strand distribution p-value = 4.53e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01

Only 100 of 266 total aligned reads displayed.

TCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC  >  NC_000913/3686683‑3686861

tCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑g                                                                                         <  1:3782023/93‑1 (MQ=255)

 cGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gt                                                                                        <  1:2120282/93‑2 (MQ=255)

  gCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtg                                                                                       >  2:5176161/1‑91 (MQ=255)

  gCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtg                                                                                       <  1:2520191/93‑3 (MQ=255)

   cTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtga                                                                                      <  1:4776464/93‑4 (MQ=255)

   cTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtga                                                                                      >  1:3367218/1‑90 (MQ=255)

    ttCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtgaa                                                                                     <  2:6411721/93‑5 (MQ=255)

    ttCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑gtgaa                                                                                     >  2:4735670/1‑89 (MQ=255)

      cGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAata                                                                                   <  2:4369532/93‑1 (MQ=255)

      cGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAata                                                                                   >  1:1863873/1‑93 (MQ=255)

        ccTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATg                                                                                 <  2:4647678/93‑1 (MQ=255)

        ccTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATg                                                                                 >  1:5147635/1‑93 (MQ=255)

        ccTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATg                                                                                 <  1:3918389/93‑1 (MQ=255)

         cTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGt                                                                                <  1:4002497/93‑1 (MQ=255)

          tAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGtt                                                                               <  2:4045968/93‑1 (MQ=255)

          tAACAGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGTCCGAGGGAAAGC‑GTGAATATGtt                                                                               <  2:1606910/93‑1 (MQ=255)

           aaCCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGttt                                                                              <  2:1532857/93‑1 (MQ=255)

           aaCCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGttt                                                                              <  2:1056674/93‑1 (MQ=255)

            aCCGAACTTGCTCCAGCAAGTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTa                                                                             >  2:6241215/1‑93 (MQ=255)

            aCCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTa                                                                             <  2:6163658/93‑1 (MQ=255)

             ccGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAg                                                                            <  2:5911157/93‑1 (MQ=255)

               gAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAgtg                                                                          >  1:1651088/1‑93 (MQ=255)

               gAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAgtg                                                                          <  2:5462603/93‑1 (MQ=255)

                   ttGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGGGTGaa                                                                      >  1:5978801/1‑93 (MQ=255)

                    tGCTCCAGCAACTGTTGCTGAGCGGGTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGATGGAAAGCGG‑GAATATGTTTAGTGTGAAt                                                                     >  1:6336650/1‑93 (MQ=255)

                      cTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAAttt                                                                   >  2:5931339/1‑93 (MQ=255)

                         cAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgcg                                                                >  1:3141230/1‑93 (MQ=255)

                         cAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTgcg                                                                >  2:2751487/1‑93 (MQ=255)

                           gCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCa                                                              >  1:4407885/1‑93 (MQ=255)

                               cTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTcc                                                          <  1:2054011/93‑1 (MQ=255)

                               cTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTcc                                                          <  2:372258/93‑1 (MQ=255)

                               cTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTcc                                                          <  1:1366059/93‑1 (MQ=255)

                               cTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTcc                                                          <  2:193719/93‑1 (MQ=255)

                                 gTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTg                                                        <  1:6224677/93‑1 (MQ=255)

                                 gTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTg                                                        <  1:4316808/93‑1 (MQ=255)

                                 gTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTg                                                        <  2:3794770/93‑1 (MQ=255)

                                  ttGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTgg                                                       <  1:5450777/93‑1 (MQ=255)

                                     cTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGccc                                                    <  2:922137/93‑1 (MQ=255)

                                       gAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCa                                                  >  2:4020278/1‑93 (MQ=255)

                                        aGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAg                                                 >  1:4069254/1‑93 (MQ=255)

                                         gCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGt                                                >  2:2308286/1‑93 (MQ=255)

                                         gCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGt                                                <  1:5277346/93‑1 (MQ=255)

                                         gCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGt                                                >  2:125649/1‑93 (MQ=255)

                                          cGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTc                                               <  1:949590/93‑1 (MQ=255)

                                            gTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAg                                             >  1:6093104/1‑93 (MQ=255)

                                            gTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAg                                             >  1:3303940/1‑93 (MQ=255)

                                             ttGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAgg                                            <  1:2626439/93‑1 (MQ=255)

                                             ttGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAgg                                            <  1:3628443/93‑1 (MQ=255)

                                             ttGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAgg                                            <  1:4057/93‑1 (MQ=255)

                                               ggTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGta                                          >  2:4906249/1‑93 (MQ=255)

                                                gTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaa                                         <  2:3887878/93‑1 (MQ=255)

                                                gTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaa                                         >  1:5623953/1‑93 (MQ=255)

                                                 tgctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaat                                        >  1:1100448/1‑93 (MQ=255)

                                                  gctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaata                                       <  1:1203434/93‑1 (MQ=255)

                                                  gctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaata                                       <  2:5110824/93‑1 (MQ=255)

                                                   ctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaataa                                      >  2:5267074/1‑93 (MQ=255)

                                                   ctgcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGtaataa                                      <  1:2291865/93‑1 (MQ=255)

                                                     gcCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACt                                    >  2:1493596/1‑93 (MQ=255)

                                                        tCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc                                 >  1:1054538/1‑93 (MQ=255)

                                                        tCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc                                 >  2:2172033/1‑93 (MQ=255)

                                                        tCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAc                                 >  2:3651921/1‑93 (MQ=255)

                                                          gACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCt                               >  1:2196046/1‑93 (MQ=255)

                                                           aCCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCtt                              >  1:865060/1‑93 (MQ=255)

                                                           aCCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCtt                              <  2:447238/93‑1 (MQ=255)

                                                           aCCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCtt                              >  2:2581897/1‑93 (MQ=255)

                                                            ccATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCttt                             <  1:5388472/93‑1 (MQ=255)

                                                            ccATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCttt                             >  1:6045476/1‑93 (MQ=255)

                                                             cATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCtttt                            >  1:6215878/1‑93 (MQ=255)

                                                             cATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCtttt                            >  2:3367851/1‑93 (MQ=255)

                                                              aTCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTg                           <  2:5697912/93‑1 (MQ=255)

                                                              aTCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTg                           <  1:3689587/93‑1 (MQ=255)

                                                               tCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt                          >  1:156434/1‑93 (MQ=255)

                                                               tCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt                          <  1:5244387/93‑1 (MQ=255)

                                                               tCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGt                          >  2:4255392/1‑93 (MQ=255)

                                                                  gCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCga                       <  2:3206265/93‑1 (MQ=255)

                                                                  gCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCga                       <  2:2327116/93‑1 (MQ=255)

                                                                   cATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgag                      >  1:4171374/1‑93 (MQ=255)

                                                                    aTGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCgaga                     >  2:4156030/1‑93 (MQ=255)

                                                                        cGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCg                 <  1:1469728/93‑1 (MQ=255)

                                                                        cGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCg                 <  2:4180500/93‑1 (MQ=255)

                                                                          gCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGa               <  1:2932450/93‑1 (MQ=255)

                                                                          gCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGa               >  2:5445930/1‑93 (MQ=255)

                                                                          gCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGa               <  2:283590/93‑1 (MQ=255)

                                                                              gACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACg           >  2:4860500/1‑93 (MQ=255)

                                                                              gACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACg           <  2:6048751/93‑1 (MQ=255)

                                                                              gACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACg           <  2:648312/93‑1 (MQ=255)

                                                                              gACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACg           >  1:4657194/1‑93 (MQ=255)

                                                                                ccGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgt         <  2:1718067/93‑1 (MQ=255)

                                                                                 cGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgtg        >  1:3677789/1‑93 (MQ=255)

                                                                                 cGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgtg        <  1:4709945/93‑1 (MQ=255)

                                                                                 cGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgtg        >  2:3055331/1‑93 (MQ=255)

                                                                                  gAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgtgt       >  2:1221204/1‑93 (MQ=255)

                                                                                  gAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGgtgt       >  1:3960751/1‑93 (MQ=255)

                                                                                   aGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGtt      >  1:717500/1‑93 (MQ=255)

                                                                                    gggAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCGCCTTTTGTCGAGAAGCGGAAACGGTGTTg     <  1:5824044/93‑1 (MQ=255)

                                                                                    gggAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTg     <  2:1312488/93‑1 (MQ=255)

                                                                                    gggAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTg     >  1:664212/1‑93 (MQ=255)

                                                                                     ggAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACCGTGTTGa    <  2:6361713/93‑1 (MQ=255)

                                                                                      gAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGAt   >  2:3299553/1‑93 (MQ=255)

                                                                                      gAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATGCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGACGCGGAAACGGTGTTGAt   <  1:2813204/93‑1 (MQ=255)

TCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC  >  NC_000913/3686683‑3686861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 30 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  NC_000913/3686682‑3686868

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G                                                                                                <  SRR4302537.2633384/94‑1 (MQ=60)

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G                                                                                                >  SRR4302537.620187/1‑94 (MQ=60)

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G                                                                                                >  SRR4302537.6287263/1‑94 (MQ=60)

 TCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑T                                                                                              <  SRR4302537.6057221/94‑1 (MQ=60)

 TCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑T                                                                                              <  SRR4302537.3808578/94‑1 (MQ=60)

 GCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCTAGACCGAGGGAAAGC‑G‑T                                                                                              <  SRR4302537.3291970/94‑1 (MQ=60)

  CGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑Gtg                                                                                              >  SRR4302537.6506163/1‑92 (MQ=60)

  CGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑Gtg                                                                                              <  SRR4302537.2135204/94‑3 (MQ=60)

   GCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑Gtga                                                                                             >  SRR4302537.3391071/1‑91 (MQ=60)

   GCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑Gtga                                                                                             <  SRR4302537.2537887/94‑4 (MQ=60)

    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAA                                                                                            >  SRR4302537.2155465/1‑94 (MQ=60)

    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAA                                                                                            >  SRR4302537.3389298/1‑94 (MQ=60)

    CTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAA                                                                                            <  SRR4302537.4809438/94‑1 (MQ=60)

      TCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCGTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATA                                                                                          <  SRR4302537.4581147/94‑1 (MQ=60)

      TCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATA                                                                                          <  SRR4302537.1369824/94‑1 (MQ=60)

      TCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATA                                                                                          <  SRR4302537.1625580/94‑1 (MQ=60)

      TCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATA                                                                                          <  SRR4302537.2261292/94‑1 (MQ=60)

      TCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATA                                                                                          >  SRR4302537.1876851/1‑94 (MQ=60)

       CGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATAT                                                                                         <  SRR4302537.4190512/94‑1 (MQ=60)

       CGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATAT                                                                                         >  SRR4302537.6108825/1‑94 (MQ=60)

        GCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATG                                                                                        <  SRR4302537.5442395/94‑1 (MQ=60)

        GCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATG                                                                                        >  SRR4302537.5183105/1‑94 (MQ=60)

         CCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGT                                                                                       <  SRR4302537.3945778/94‑1 (MQ=60)

          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTT                                                                                      <  SRR4302537.4073650/94‑1 (MQ=60)

          CTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTT                                                                                      <  SRR4302537.4030517/94‑1 (MQ=60)

           TAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTT                                                                                     >  SRR4302537.3709148/1‑94 (MQ=60)

           TAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTT                                                                                     <  SRR4302537.4828529/94‑1 (MQ=60)

            AACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTA                                                                                    >  SRR4302537.1281509/1‑94 (MQ=60)

             ACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAG                                                                                   <  SRR4302537.1607529/94‑1 (MQ=60)

               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTG                                                                                 >  SRR4302537.3977304/1‑94 (MQ=60)

               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTG                                                                                 >  SRR4302537.1545858/1‑94 (MQ=60)

               CGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTG                                                                                 >  SRR4302537.1662668/1‑94 (MQ=60)

                GAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGT                                                                                >  SRR4302537.5307368/1‑94 (MQ=60)

                  ACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGA                                                                              >  SRR4302537.2680121/1‑94 (MQ=60)

                   CTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAA                                                                             >  SRR4302537.2398609/1‑94 (MQ=60)

                   CTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGGGTGAA                                                                             >  SRR4302537.6019840/1‑94 (MQ=60)

                    TTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAAT                                                                            <  SRR4302537.1849329/94‑1 (MQ=60)

                    TTGCTCCAGCAACTGTTGCTGAGCGGGTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGATGGAAAGCGG‑GAATATGTTTAGTGTGAAT                                                                            >  SRR4302537.6379963/1‑94 (MQ=60)

                     TGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATT                                                                           <  SRR4302537.5374188/94‑1 (MQ=60)

                      GCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTT                                                                          <  SRR4302537.4003802/94‑1 (MQ=60)

                      GCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTT                                                                          <  SRR4302537.229611/94‑1 (MQ=60)

                      GCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTT                                                                          <  SRR4302537.2213253/94‑1 (MQ=60)

                       CTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTG                                                                         <  SRR4302537.4086095/94‑1 (MQ=60)

                        TCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGC                                                                        <  SRR4302537.5406537/94‑1 (MQ=60)

                         CCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCG                                                                       >  SRR4302537.3163275/1‑94 (MQ=60)

                           AGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCA                                                                     >  SRR4302537.4438306/1‑94 (MQ=60)

                             CAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATT                                                                   <  SRR4302537.707618/94‑1 (MQ=60)

                             CAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATT                                                                   >  SRR4302537.6182058/1‑94 (MQ=60)

                             CAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATT                                                                   >  SRR4302537.2852846/1‑94 (MQ=60)

                             CAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATT                                                                   <  SRR4302537.593404/94‑1 (MQ=60)

                              AACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTC                                                                  >  SRR4302537.345418/1‑94 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                >  SRR4302537.2796014/1‑94 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302537.1375576/94‑1 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302537.5251981/94‑1 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302537.4743457/94‑1 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302537.3703267/94‑1 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302537.1773231/94‑1 (MQ=60)

                                CTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCT                                                                <  SRR4302537.2068422/94‑1 (MQ=60)

                                  GTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGG                                                              <  SRR4302537.4346818/94‑1 (MQ=60)

                                  GTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGG                                                              <  SRR4302537.6267514/94‑1 (MQ=60)

                                   TTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGC                                                             <  SRR4302537.5487957/94‑1 (MQ=60)

                                    TGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCC                                                            >  SRR4302537.4691582/1‑94 (MQ=60)

                                        GAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAG                                                        >  SRR4302537.2612995/1‑94 (MQ=60)

                                        GAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAG                                                        <  SRR4302537.5657674/94‑1 (MQ=60)

                                        GAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAG                                                        >  SRR4302537.4097677/1‑94 (MQ=60)

                                        GAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAG                                                        >  SRR4302537.4133803/1‑94 (MQ=60)

                                         AGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGT                                                       <  SRR4302537.4278110/94‑1 (MQ=60)

                                          GCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTC                                                      <  SRR4302537.5313658/94‑1 (MQ=60)

                                           CGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCA                                                     <  SRR4302537.956155/94‑1 (MQ=60)

                                           CGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCA                                                     <  SRR4302537.1471846/94‑1 (MQ=60)

                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    <  SRR4302537.4513179/94‑1 (MQ=60)

                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    >  SRR4302537.3327298/1‑94 (MQ=60)

                                            GGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAG                                                    >  SRR4302537.6134972/1‑94 (MQ=60)

                                             GTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGG                                                   <  SRR4302537.5679271/94‑1 (MQ=60)

                                             GTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTAAGG                                                   >  SRR4302537.5225346/1‑94 (MQ=60)

                                             GTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTACTGGCCCCAGTCAGG                                                   >  SRR4302537.787285/1‑94 (MQ=60)

                                              TTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGT                                                  <  SRR4302537.3654018/94‑1 (MQ=60)

                                              TTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGT                                                  <  SRR4302537.2644839/94‑1 (MQ=60)

                                              TTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGT                                                  >  SRR4302537.2155669/1‑94 (MQ=60)

                                              TTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGT                                                  <  SRR4302537.4091/94‑1 (MQ=60)

                                                GGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAA                                                <  SRR4302537.4422068/94‑1 (MQ=60)

                                                GGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAA                                                >  SRR4302537.5662414/1‑94 (MQ=60)

                                                GGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAA                                                <  SRR4302537.3992589/94‑1 (MQ=60)

                                                 GTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAAT                                               >  SRR4302537.1108054/1‑94 (MQ=60)

                                                  TGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATA                                              <  SRR4302537.3666775/94‑1 (MQ=60)

                                                   GCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAA                                             <  SRR4302537.1211808/94‑1 (MQ=60)

                                                    CTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAC                                            <  SRR4302537.2308046/94‑1 (MQ=60)

                                                    CTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAC                                            >  SRR4302537.662526/1‑94 (MQ=60)

                                                    CTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAAC                                            >  SRR4302537.1980169/1‑94 (MQ=60)

                                                     TGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACT                                           >  SRR4302537.260684/1‑94 (MQ=60)

                                                     TGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACT                                           >  SRR4302537.3868051/1‑94 (MQ=60)

                                                      GCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTC                                          <  SRR4302537.5894693/94‑1 (MQ=60)

                                                        CTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAC                                        >  SRR4302537.1061814/1‑94 (MQ=60)

                                                        CTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCAC                                        >  SRR4302537.5441428/1‑94 (MQ=60)

                                                          CGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCT                                      <  SRR4302537.6259030/94‑1 (MQ=60)

                                                          CGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCT                                      >  SRR4302537.2211521/1‑94 (MQ=60)

                                                           GACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGACTTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTT                                     <  SRR4302537.4698150/94‑1 (MQ=60)

                                                           GACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTT                                     >  SRR4302537.870995/1‑94 (MQ=60)

                                                           GACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTT                                     >  SRR4302537.4683558/1‑94 (MQ=60)

                                                            ACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTT                                    >  SRR4302537.6086996/1‑94 (MQ=60)

                                                             CCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTT                                   >  SRR4302537.6258677/1‑94 (MQ=60)

                                                             CCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTT                                   >  SRR4302537.732234/1‑94 (MQ=60)

                                                             CCATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTT                                   <  SRR4302537.5425295/94‑1 (MQ=60)

                                                              CATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTG                                  <  SRR4302537.6316662/94‑1 (MQ=60)

                                                               ATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGT                                 <  SRR4302537.3715542/94‑1 (MQ=60)

                                                               ATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGT                                 >  SRR4302537.157548/1‑94 (MQ=60)

                                                               ATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGT                                 >  SRR4302537.1211701/1‑94 (MQ=60)

                                                               ATCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATGCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGT                                 <  SRR4302537.3746352/94‑1 (MQ=60)

                                                                TCGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTC                                <  SRR4302537.5280474/94‑1 (MQ=60)

                                                                 CGGCATGACGGCCAGACCGAGTGAAAGC‑GTTAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCG                               <  SRR4302537.1508397/94‑1 (MQ=60)

                                                                 CGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCG                               <  SRR4302537.317270/94‑1 (MQ=60)

                                                                 CGGCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCG                               >  SRR4302537.4201886/1‑94 (MQ=60)

                                                                   GCATGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAG                             >  SRR4302537.4200507/1‑94 (MQ=60)

                                                                      TGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATACCTCACCTTTTGTCGAGAAG                          <  SRR4302537.3394005/94‑1 (MQ=60)

                                                                      TGACGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAG                          <  SRR4302537.1186118/94‑1 (MQ=60)

                                                                         CGGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGG                       <  SRR4302537.1479949/94‑1 (MQ=60)

                                                                          GGCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGA                      <  SRR4302537.3900417/94‑1 (MQ=60)

                                                                           GCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAA                     <  SRR4302537.2953042/94‑1 (MQ=60)

                                                                           GCCAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAA                     <  SRR4302537.3135686/94‑1 (MQ=60)

                                                                             CAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAC                   <  SRR4302537.932080/94‑1 (MQ=60)

                                                                             CAGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAAC                   <  SRR4302537.5118542/94‑1 (MQ=60)

                                                                              AGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACG                  >  SRR4302537.872504/1‑94 (MQ=60)

                                                                              AGACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACG                  >  SRR4302537.4689392/1‑94 (MQ=60)

                                                                                ACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGT                <  SRR4302537.2122370/94‑1 (MQ=60)

                                                                                ACCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGT                >  SRR4302537.6126123/1‑94 (MQ=60)

                                                                                 CCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAACCGGTG               >  SRR4302537.3800064/1‑94 (MQ=60)

                                                                                 CCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTG               >  SRR4302537.3703662/1‑94 (MQ=60)

                                                                                 CCGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTG               >  SRR4302537.5711068/1‑94 (MQ=60)

                                                                                  CGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              >  SRR4302537.3988457/1‑94 (MQ=60)

                                                                                  CGAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGT              <  SRR4302537.4742530/94‑1 (MQ=60)

                                                                                   GAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTT             >  SRR4302537.722400/1‑94 (MQ=60)

                                                                                   GAGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTT             <  SRR4302537.3100646/94‑1 (MQ=60)

                                                                                    AGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTG            >  SRR4302537.668737/1‑94 (MQ=60)

                                                                                    AGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTG            >  SRR4302537.107430/1‑94 (MQ=60)

                                                                                    AGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTG            >  SRR4302537.1441314/1‑94 (MQ=60)

                                                                                    AGGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTG            >  SRR4302537.1897527/1‑94 (MQ=60)

                                                                                     GGGAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCGCCTTTTGTCGAGAAGCGGAAACGGTGTTGA           <  SRR4302537.5863958/94‑1 (MQ=60)

                                                                                       GAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATC         <  SRR4302537.40457/94‑1 (MQ=60)

                                                                                       GAAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATGCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGACGCGGAAACGGTGTTGATC         <  SRR4302537.2832874/94‑1 (MQ=60)

                                                                                        AAAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCC        >  SRR4302537.4996896/1‑94 (MQ=60)

                                                                                         AAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCC       <  SRR4302537.4516915/94‑1 (MQ=60)

                                                                                         AAGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCC       <  SRR4302537.266596/94‑1 (MQ=60)

                                                                                          AGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      <  SRR4302537.1715071/94‑1 (MQ=60)

                                                                                          AGC‑GTGAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCA      <  SRR4302537.1972174/94‑1 (MQ=60)

                                                                                              T‑GAATATGTTTAGTGTGAATTTGCTCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  SRR4302537.2449726/1‑94 (MQ=60)

GTCGCTTCGCCTAACCGAACTTGCTCCAGCAACTGTTGCTGAGCGGTTGGTGCTGCCTCGACCATCGGCATGACGGCCAGACCGAGGGAAAGC‑G‑GAATATGTTTAGTGTGAATTTGCGCATTCCTGGCCCCAGTCAGGTAATAACTCACCTTTTGTCGAGAAGCGGAAACGGTGTTGATCCCAGCCT  >  NC_000913/3686682‑3686868

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`