Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,781,277 A→G R4R (CGT→CGC)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,781,2770AG99.3% 2612.6 / 1.0 755R4R (CGT→CGC) gpsAglycerol‑3‑phosphate dehydrogenase (NAD+)
Reads supporting (aligned to +/- strand):  ref base A (1/4);  new base G (375/375);  total (376/379)
Fisher's exact test for biased strand distribution p-value = 3.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.59e-01

Only 100 of 786 total aligned reads displayed.

GCCCCAGAGGACAACCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTACGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCATCGAAGTTAACCG  >  NC_000913/3781184‑3781367
                                                                                             |                                                                                          
gCCCCAGAGGACAACCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTa                                                                                            >  2:10097467/1‑94 (MQ=255)
  cccAGAGGACAACCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCg                                                                                          >  2:7876250/1‑94 (MQ=255)
    cAGAGGACATCCTCGTGGCTGTTTCTTGCCAGGGTGATGGCAAGAGCGTTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGtt                                                                                        <  2:4568638/94‑1 (MQ=255)
    cAGAGGACAACCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGtt                                                                                        >  1:1039771/1‑94 (MQ=255)
     agagGACACCCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTg                                                                                       <  1:3931049/94‑1 (MQ=255)
     agagGACAACCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTg                                                                                       <  2:3584259/94‑1 (MQ=255)
       agGACACCCTCGTGGCCATTTCTTGCCAGGGTGATGGAAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGt                                                                                     <  2:9251841/94‑1 (MQ=255)
        ggACAACCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGtt                                                                                    >  2:9894461/1‑94 (MQ=255)
        ggACAACCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGtt                                                                                    >  1:8815058/1‑94 (MQ=255)
        ggACAACCGCGTGGCGATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGtt                                                                                    <  1:803221/94‑1 (MQ=255)
         gACAACCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTtc                                                                                   <  1:703730/94‑1 (MQ=255)
           cAACCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTtcat                                                                                 <  2:9671683/94‑1 (MQ=255)
            aaCCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTtcatc                                                                                >  2:4878876/1‑94 (MQ=255)
              ccTCGGGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAg                                                                              <  2:2065988/94‑1 (MQ=255)
               cTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAgg                                                                             <  1:351120/94‑1 (MQ=255)
               cTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAgg                                                                             >  1:367688/1‑94 (MQ=255)
               cTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAgg                                                                             <  2:10390156/94‑1 (MQ=255)
               cTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAgg                                                                             >  2:1947007/1‑94 (MQ=255)
                tCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGc                                                                            >  2:6674403/1‑94 (MQ=255)
                tCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGc                                                                            >  1:6325303/1‑94 (MQ=255)
                  gTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCAt                                                                          <  1:9844071/94‑1 (MQ=255)
                   tGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATc                                                                         >  1:6593404/1‑94 (MQ=255)
                   gggCCATTTCTTACCAGGGTAGTGTCAAGAGCGGTTCCGAACGATCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATc                                                                         <  1:7278580/93‑1 (MQ=255)
                     gCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCt                                                                       <  1:6057252/94‑1 (MQ=255)
                      ccATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTg                                                                      >  1:4757679/1‑94 (MQ=255)
                      ccATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTAAGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAAGCATCCTg                                                                      >  2:2365406/1‑94 (MQ=255)
                        aTTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGAt                                                                    >  1:8516343/1‑94 (MQ=255)
                         tttCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATg                                                                   <  2:5292239/94‑1 (MQ=255)
                         tttCTTCCCGGGGTGATGGAAGGAGCGGTGCCTTGCGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATg                                                                   <  2:4063737/94‑1 (MQ=255)
                           tCTTGCCGGGGTGATGGCAAGAGCGGTGCTGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGtt                                                                 <  1:8334071/94‑1 (MQ=255)
                           tCTTCCCAGGTTGATGGAAGGAGTGGGGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGtt                                                                 <  1:889470/94‑1 (MQ=255)
                            cTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGttc                                                                >  1:5268988/1‑94 (MQ=255)
                              tGCCAGGTTGATGGCAAGAGCGGTGCCGTCCGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGttctt                                                              <  2:4373021/94‑1 (MQ=255)
                              tGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGttctt                                                              >  1:9698096/1‑94 (MQ=255)
                                ccAGGGTGATGGCAAGAGCGGTGCCGTACGAGGCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGGTCATCAGGCCTCCTGATGTTCTTCa                                                            >  1:4320812/1‑94 (MQ=255)
                                ccAGGGTGATGGCAAGAGCGGTCCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCAGTGCGTTGGTTCATCAGGCAGCCTGATGTTCTTCa                                                            <  1:5503517/94‑1 (MQ=255)
                                 cAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAg                                                           >  1:6855239/1‑94 (MQ=255)
                                  aGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAga                                                          >  2:5662556/1‑93 (MQ=255)
                                     gTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTAcc                                                       >  1:311535/1‑94 (MQ=255)
                                      tGATGGCAAGAGCGGTGCCGTCCGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCt                                                      <  2:8969009/94‑1 (MQ=255)
                                      ggATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCt                                                      <  1:10014970/93‑1 (MQ=255)
                                        aTGGCAAGAGGCGGGCCGGACGAGCCGGCACTGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTc                                                    <  2:1161737/94‑1 (MQ=255)
                                         gggaaaGAGCGGTCCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCg                                                   <  2:7161379/90‑1 (MQ=255)
                                           gCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGcc                                                 <  1:6677174/94‑1 (MQ=255)
                                            cAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCa                                                <  1:3079487/94‑1 (MQ=255)
                                            cAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCa                                                <  2:6822928/94‑1 (MQ=255)
                                             aaGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAg                                               <  2:6312401/94‑1 (MQ=255)
                                             aaGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAg                                               >  2:8089451/1‑94 (MQ=255)
                                                agCGGTGCGGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCct                                            <  2:2976926/94‑1 (MQ=255)
                                                agCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCct                                            <  2:3083956/94‑1 (MQ=255)
                                                agCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCct                                            >  2:954862/1‑94 (MQ=255)
                                                  gggTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCctgc                                          <  1:3293553/93‑1 (MQ=255)
                                                   ggTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCctgct                                         >  1:4168062/1‑94 (MQ=255)
                                                    gTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAATACCTTCGCCCGCctgctc                                        >  1:8732958/1‑93 (MQ=255)
                                                      gCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCctgctgct                                      <  2:7548650/94‑1 (MQ=255)
                                                       ccGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCctgctgctg                                     >  2:7668903/1‑94 (MQ=255)
                                                        cGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCtgctgctgc                                    >  1:7887680/1‑94 (MQ=255)
                                                         gTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCa                                   >  2:6058177/1‑94 (MQ=255)
                                                         gTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCa                                   >  2:9402636/1‑94 (MQ=255)
                                                         gTACGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCa                                   >  1:1138156/1‑94 (MQ=255)
                                                            cGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAAt                                >  2:8885064/1‑94 (MQ=255)
                                                            cGAGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAAt                                <  2:4674248/94‑1 (MQ=255)
                                                              aGCCGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAg                              >  1:6449194/1‑94 (MQ=255)
                                                                 cGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTc                           >  1:1845824/1‑94 (MQ=255)
                                                                 cGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTc                           >  2:6456661/1‑94 (MQ=255)
                                                                 cGGCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTc                           <  1:2761578/94‑1 (MQ=255)
                                                                  ggCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCa                          <  2:2781962/94‑1 (MQ=255)
                                                                  ggCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCa                          <  1:1364164/94‑1 (MQ=255)
                                                                  ggCACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCa                          <  1:2176484/94‑1 (MQ=255)
                                                                    cACCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATg                        <  2:1312678/94‑1 (MQ=255)
                                                                    cACCGACTACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATg                        <  1:2896243/94‑1 (MQ=255)
                                                                     aCCGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGa                       <  2:7410075/94‑1 (MQ=255)
                                                                      ccGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGGTGCAAATAGTTCATGaa                      >  1:2445392/1‑94 (MQ=255)
                                                                      ccGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGaa                      >  2:9123141/1‑94 (MQ=255)
                                                                      ccGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGaa                      <  2:1658682/94‑1 (MQ=255)
                                                                      ccGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGaa                      <  1:7737638/94‑1 (MQ=255)
                                                                      ccGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGaa                      <  1:4847945/94‑1 (MQ=255)
                                                                      ccGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGaa                      <  2:8348033/94‑1 (MQ=255)
                                                                      ccGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGaa                      >  2:5785008/1‑94 (MQ=255)
                                                                       cGATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAAc                     >  2:1442459/1‑94 (MQ=255)
                                                                        gATCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACa                    >  2:3928800/1‑94 (MQ=255)
                                                                         agcacaGCCATTGAAGCAATGCGCCGGGTCATCAGGCAACCGGACGATCTGCAGTACCTGCGCCAGCCTGCTGCGGCAAATAGGGCACGAACaa                   >  1:9355698/3‑93 (MQ=255)
                                                                         aTCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAg                   <  1:515546/94‑1 (MQ=255)
                                                                         aTCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAg                   >  1:7513870/1‑94 (MQ=255)
                                                                         aTCACAGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAg                   <  2:3256369/94‑1 (MQ=255)
                                                                            acaGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAgcgc                >  2:5549227/1‑94 (MQ=255)
                                                                             caGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCa               >  2:3752417/1‑94 (MQ=255)
                                                                             caGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCa               >  2:1590343/1‑94 (MQ=255)
                                                                              aGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCAt              <  1:5737942/94‑1 (MQ=255)
                                                                              aGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCAt              <  2:2647995/94‑1 (MQ=255)
                                                                              aGTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCAt              <  1:1666420/94‑1 (MQ=255)
                                                                               gTCATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCATc             <  2:5904059/94‑1 (MQ=255)
                                                                                 cATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCATCGa           <  1:4928584/94‑1 (MQ=255)
                                                                                 cATTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCATCGa           >  2:5071334/1‑94 (MQ=255)
                                                                                  aTTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCATCGaa          >  1:10196444/1‑94 (MQ=255)
                                                                                  aTTGAAGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCATCGaa          <  1:2624881/94‑1 (MQ=255)
                                                                                      aaGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTTATGAACAGCGCATCGAAGTTa      >  1:9070822/1‑94 (MQ=255)
                                                                                       aGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCATCGAAGTTac     >  2:8605037/1‑93 (MQ=255)
                                                                                       aGCATTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCATCGAAGTTaa     <  2:2192028/94‑1 (MQ=255)
                                                                                          aTTGCGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCATCGAAGTTAACCg  <  2:6971483/94‑1 (MQ=255)
                                                                                             |                                                                                          
GCCCCAGAGGACAACCTCGTGGCCATTTCTTGCCAGGGTGATGGCAAGAGCGGTGCCGTACGAGCCGGCACCGATCACAGTCATTGAAGCATTACGTTGGTTCATCAGGCATCCTGATGTTCTTCAGTACCTTCGCCAGCCTGCTGCTGCAAATAGTTCATGAACAGCGCATCGAAGTTAACCG  >  NC_000913/3781184‑3781367

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 372 total aligned reads displayed.
AAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGT  >  NC_000913/3784142‑3784328
                                                                                             |                                                                                             
AAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCG                                                                                               <  SRR4302545.373654/94‑1 (MQ=60)
 AGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGG                                                                                              >  SRR4302545.2852183/1‑94 (MQ=60)
   CGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGAT                                                                                            <  SRR4302545.7722/94‑1 (MQ=60)
    GCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATG                                                                                           <  SRR4302545.6613468/94‑1 (MQ=60)
    GCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATG                                                                                           >  SRR4302545.1398171/1‑94 (MQ=60)
    GCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATG                                                                                           <  SRR4302545.4418750/94‑1 (MQ=60)
    GCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATG                                                                                           <  SRR4302545.565385/94‑1 (MQ=60)
    GCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATG                                                                                           >  SRR4302545.5713633/1‑94 (MQ=60)
     CTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGC                                                                                          >  SRR4302545.6793916/1‑94 (MQ=60)
     CTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGC                                                                                          <  SRR4302545.4181042/94‑1 (MQ=60)
      TGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGATAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCG                                                                                         >  SRR4302545.6152443/1‑94 (MQ=60)
      TGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGAG                                                                                         <  SRR4302545.8494024/94‑1 (MQ=60)
       GGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGC                                                                                        >  SRR4302545.8210565/1‑94 (MQ=60)
        GATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCG                                                                                       <  SRR4302545.7540307/94‑1 (MQ=60)
        GATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGGGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCG                                                                                       >  SRR4302545.8038382/1‑94 (MQ=60)
        GATCACTGCGTGGAAGAAGTCGCGAAAGCGGATGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCG                                                                                       >  SRR4302545.10899547/1‑94 (MQ=60)
         ATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGA                                                                                      >  SRR4302545.67089/1‑94 (MQ=60)
         ATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGA                                                                                      <  SRR4302545.2013340/94‑1 (MQ=60)
          TCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGAC                                                                                     >  SRR4302545.2989313/1‑94 (MQ=60)
           CACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGATGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGatgtc                                                                                    >  SRR4302545.7492183/1‑89 (MQ=60)
            ACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCC                                                                                   >  SRR4302545.6640596/1‑94 (MQ=60)
            ACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCC                                                                                   >  SRR4302545.2022858/1‑94 (MQ=60)
            ACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCC                                                                                   >  SRR4302545.3115568/1‑94 (MQ=60)
            ACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCC                                                                                   <  SRR4302545.3464859/94‑1 (MQ=60)
            ACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCA                                                                                   <  SRR4302545.542752/94‑1 (MQ=60)
             CTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCG                                                                                  <  SRR4302545.8755662/94‑1 (MQ=60)
             CTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCG                                                                                  >  SRR4302545.3262256/1‑94 (MQ=60)
             CTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCG                                                                                  <  SRR4302545.4239248/94‑1 (MQ=60)
             CTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCG                                                                                  <  SRR4302545.10109394/94‑1 (MQ=60)
              TGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGG                                                                                 >  SRR4302545.7394030/1‑94 (MQ=60)
              TGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGG                                                                                 >  SRR4302545.7798728/1‑94 (MQ=60)
              TGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGG                                                                                 <  SRR4302545.57889/94‑1 (MQ=60)
               GCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGC                                                                                <  SRR4302545.120875/94‑1 (MQ=60)
                CGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCA                                                                               >  SRR4302545.8942238/1‑94 (MQ=60)
                 GTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAA                                                                              >  SRR4302545.6421463/1‑94 (MQ=60)
                 GTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAA                                                                              >  SRR4302545.2899422/1‑94 (MQ=60)
                  GGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAAC                                                                             <  SRR4302545.2003865/94‑1 (MQ=60)
                   GGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACG                                                                            <  SRR4302545.7382644/94‑1 (MQ=60)
                   GGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACG                                                                            >  SRR4302545.3580687/1‑94 (MQ=60)
                   GGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACG                                                                            >  SRR4302545.5784962/1‑94 (MQ=60)
                    GAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGG                                                                           >  SRR4302545.197685/1‑94 (MQ=60)
                    GAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGG                                                                           >  SRR4302545.10027930/1‑94 (MQ=60)
                     AAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGG                                                                          <  SRR4302545.3434295/94‑1 (MQ=60)
                     AAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGG                                                                          <  SRR4302545.7793303/94‑1 (MQ=60)
                     AAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGG                                                                          >  SRR4302545.8540418/1‑94 (MQ=60)
                      AGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGT                                                                         <  SRR4302545.3303160/94‑1 (MQ=60)
                      AGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGT                                                                         >  SRR4302545.8578831/1‑94 (MQ=60)
                       GAAGTCGCGAAAGCGTTTGAGTCCGTGGGTGGACAACTGCTGATCCCCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTC                                                                        <  SRR4302545.4525073/94‑1 (MQ=60)
                       GAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTC                                                                        >  SRR4302545.8092066/1‑94 (MQ=60)
                       GAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTC                                                                        <  SRR4302545.1284412/94‑1 (MQ=60)
                        AAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCA                                                                       >  SRR4302545.8219255/1‑94 (MQ=60)
                         AGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAG                                                                      <  SRR4302545.10284250/94‑1 (MQ=60)
                         AGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAG                                                                      <  SRR4302545.4703566/94‑1 (MQ=60)
                         AGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAG                                                                      <  SRR4302545.5691046/94‑1 (MQ=60)
                         AGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGGAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAG                                                                      >  SRR4302545.8321622/1‑94 (MQ=60)
                          GTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGG                                                                     >  SRR4302545.2777132/1‑94 (MQ=60)
                          GTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGG                                                                     <  SRR4302545.1736817/94‑1 (MQ=60)
                          GTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGG                                                                     >  SRR4302545.9604048/1‑94 (MQ=60)
                          GTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGG                                                                     <  SRR4302545.6714848/94‑1 (MQ=60)
                          GTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGG                                                                     <  SRR4302545.4662150/94‑1 (MQ=60)
                          GTCGCGAAAGCGGTTGAGTCCGTGGGGGGACAACTGCGGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGG                                                                     >  SRR4302545.3573551/1‑94 (MQ=60)
                            CGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCA                                                                   <  SRR4302545.1509164/94‑1 (MQ=60)
                             GCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCAC                                                                  <  SRR4302545.924968/94‑1 (MQ=60)
                             GCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCAC                                                                  >  SRR4302545.3891118/1‑94 (MQ=60)
                              CGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACC                                                                 >  SRR4302545.6675547/1‑94 (MQ=60)
                               GAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACAC                                                                <  SRR4302545.1365130/94‑1 (MQ=60)
                                AAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACA                                                               >  SRR4302545.8671089/1‑94 (MQ=60)
                                 AAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACAC                                                              <  SRR4302545.2138068/94‑1 (MQ=60)
                                  AGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACG                                                             <  SRR4302545.970651/94‑1 (MQ=60)
                                  AGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACG                                                             >  SRR4302545.3769085/1‑94 (MQ=60)
                                  AGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACG                                                             >  SRR4302545.1629642/1‑94 (MQ=60)
                                     GGTTGAGTCCGTGGGTGTTCAACTTCTGATCACCGCTGACCACTTTAACGCTGAGCGGATGCGCGTTCCGGCAACGGGTCAGGCACTCACGGCA                                                          >  SRR4302545.9637952/1‑94 (MQ=60)
                                     GGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCA                                                          >  SRR4302545.8922623/1‑94 (MQ=60)
                                     GGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCA                                                          >  SRR4302545.10179040/1‑94 (MQ=60)
                                     GGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCA                                                          >  SRR4302545.1329578/1‑94 (MQ=60)
                                     GGTTGAGTCCGTGGGCGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCA                                                          >  SRR4302545.5877336/1‑94 (MQ=60)
                                       TTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACA                                                        >  SRR4302545.1844820/1‑94 (MQ=60)
                                       TTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACA                                                        <  SRR4302545.8513692/94‑1 (MQ=60)
                                        TGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACAC                                                       >  SRR4302545.576639/1‑94 (MQ=60)
                                        TGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACAC                                                       <  SRR4302545.5576746/94‑1 (MQ=60)
                                         GAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACA                                                      >  SRR4302545.4205732/1‑94 (MQ=60)
                                          AGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACAC                                                     >  SRR4302545.2752745/1‑94 (MQ=60)
                                           GTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACC                                                    <  SRR4302545.4546927/94‑1 (MQ=60)
                                           GTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACA                                                    >  SRR4302545.9100694/1‑94 (MQ=60)
                                            TCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCA                                                   >  SRR4302545.1993657/1‑94 (MQ=60)
                                            TCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCA                                                   >  SRR4302545.8804842/1‑94 (MQ=60)
                                            TCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCA                                                   >  SRR4302545.7789525/1‑94 (MQ=60)
                                            TCCGTGGGTGGACAACGTCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCA                                                   <  SRR4302545.5919546/94‑1 (MQ=60)
                                              CGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAAC                                                 >  SRR4302545.363256/1‑94 (MQ=60)
                                              CGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAAC                                                 >  SRR4302545.5078548/1‑94 (MQ=60)
                                              CGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAAC                                                 <  SRR4302545.9753010/94‑1 (MQ=60)
                                              CGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAAC                                                 <  SRR4302545.4389453/94‑1 (MQ=60)
                                                gtGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCT                                               <  SRR4302545.10625990/92‑1 (MQ=60)
                                                aaggagacCAACTGCTGATAACCGCTGACCACGGTAACGCAGAGCGGTTGCACGGTCCGGCAACGGGTAAAGCACAAACGGAACACACCAACCT                                               <  SRR4302545.9644646/86‑1 (MQ=28)
                                                TGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCT                                               <  SRR4302545.3688799/94‑1 (MQ=60)
                                                 GGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTG                                              <  SRR4302545.8216485/94‑1 (MQ=60)
                                                 GGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTG                                              <  SRR4302545.2958251/94‑1 (MQ=60)
                                                 GGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTG                                              <  SRR4302545.2653675/94‑1 (MQ=60)
                                                  GGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGC                                             <  SRR4302545.2571192/94‑1 (MQ=60)
                                                  GGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGC                                             <  SRR4302545.3243145/94‑1 (MQ=60)
                                                  GGTAGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGC                                             >  SRR4302545.9312573/1‑94 (MQ=60)
                                                   GTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCC                                            <  SRR4302545.455046/94‑1 (MQ=60)
                                                   GTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCC                                            <  SRR4302545.6279725/94‑1 (MQ=60)
                                                   GTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCC                                            >  SRR4302545.1947645/1‑94 (MQ=60)
                                                   GTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCC                                            >  SRR4302545.9420151/1‑94 (MQ=60)
                                                    TGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCA                                           >  SRR4302545.8596406/1‑94 (MQ=60)
                                                     GGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAG                                          <  SRR4302545.9964258/94‑1 (MQ=60)
                                                      GACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCATGCACACACGGCACACACCAACCTGCCAGT                                         <  SRR4302545.1572225/94‑1 (MQ=60)
                                                      GACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGT                                         >  SRR4302545.9114918/1‑94 (MQ=60)
                                                      GACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGT                                         <  SRR4302545.6545847/94‑1 (MQ=60)
                                                       ACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTT                                        >  SRR4302545.5211744/1‑94 (MQ=60)
                                                        CAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTC                                       <  SRR4302545.6106096/94‑1 (MQ=60)
                                                         AACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCC                                      >  SRR4302545.4114887/1‑94 (MQ=60)
                                                         AACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCC                                      >  SRR4302545.5316623/1‑94 (MQ=60)
                                                         AACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCC                                      >  SRR4302545.3060400/1‑94 (MQ=60)
                                                         AACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCC                                      <  SRR4302545.4009432/94‑1 (MQ=60)
                                                          ACTGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCG                                     >  SRR4302545.9206495/1‑94 (MQ=60)
                                                            TGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCT                                   >  SRR4302545.6982899/1‑94 (MQ=60)
                                                            TGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCT                                   >  SRR4302545.6376438/1‑94 (MQ=60)
                                                            TGCTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCT                                   <  SRR4302545.2412065/94‑1 (MQ=60)
                                                            GGCTGATCACCGCTACCCACGGTACCGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCT                                   <  SRR4302545.2885861/94‑1 (MQ=60)
                                                              gtgacccccgcggaccgCGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACCCCACCCTGCCAGTTCCGCTGA                                 <  SRR4302545.9148367/77‑1 (MQ=60)
                                                              CTGATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGA                                 >  SRR4302545.6330667/1‑94 (MQ=60)
                                                                TATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATT                               >  SRR4302545.4071512/1‑94 (MQ=60)
                                                                GATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATT                               >  SRR4302545.9618563/1‑94 (MQ=60)
                                                                 ATCACCTCCGGCCACGGAAACGCTGAGCGGATGCGCGATGCGGCGACGGGTTAGGCTCACACGGCACACACCAACCTGCCAGTTCCGCTGCTTT                              >  SRR4302545.4272854/1‑94 (MQ=53)
                                                                 ATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGTGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTT                              >  SRR4302545.3342315/1‑94 (MQ=60)
                                                                 ATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTT                              >  SRR4302545.9451254/1‑94 (MQ=60)
                                                                 ATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTT                              >  SRR4302545.9572276/1‑94 (MQ=60)
                                                                 ATCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTT                              >  SRR4302545.10898819/1‑94 (MQ=60)
                                                                  TCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTA                             >  SRR4302545.9547336/1‑94 (MQ=60)
                                                                  TCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTA                             >  SRR4302545.7750284/1‑94 (MQ=60)
                                                                  TCACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTA                             >  SRR4302545.7180737/1‑94 (MQ=60)
                                                                   CACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTAC                            <  SRR4302545.9839572/94‑1 (MQ=60)
                                                                   CACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTAC                            <  SRR4302545.2323485/94‑1 (MQ=60)
                                                                   CACCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTAC                            >  SRR4302545.4780931/1‑94 (MQ=60)
                                                                    CCCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACG                           <  SRR4302545.2027941/94‑1 (MQ=60)
                                                                     CCGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGT                          <  SRR4302545.5501785/94‑1 (MQ=60)
                                                                      CGCTGCCCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTT                         <  SRR4302545.10365014/94‑1 (MQ=60)
                                                                      CGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTT                         <  SRR4302545.883198/94‑1 (MQ=60)
                                                                      CGCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTT                         <  SRR4302545.10318043/94‑1 (MQ=60)
                                                                       GCTGACCACGGTAACGCTGAGCGGATGCGCGCTCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTG                        >  SRR4302545.4612890/1‑94 (MQ=60)
                                                                       GCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTG                        >  SRR4302545.1068060/1‑94 (MQ=60)
                                                                       GCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTG                        <  SRR4302545.9932264/94‑1 (MQ=60)
                                                                       GCTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTG                        >  SRR4302545.5290353/1‑94 (MQ=60)
                                                                        CTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGG                       >  SRR4302545.10823253/1‑94 (MQ=60)
                                                                        CTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGG                       >  SRR4302545.6898629/1‑94 (MQ=60)
                                                                        CTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGG                       >  SRR4302545.10913729/1‑94 (MQ=60)
                                                                        CTGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGCTCCGCTGGTTTACGTTGG                       >  SRR4302545.4003394/1‑94 (MQ=60)
                                                                         TGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGT                      <  SRR4302545.1006135/94‑1 (MQ=60)
                                                                         TGACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGT                      >  SRR4302545.5006419/1‑94 (MQ=60)
                                                                          GACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTG                     >  SRR4302545.792242/1‑94 (MQ=60)
                                                                          GACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTG                     <  SRR4302545.4967436/94‑1 (MQ=60)
                                                                          GACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTG                     <  SRR4302545.8351274/94‑1 (MQ=60)
                                                                          GACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTG                     <  SRR4302545.2246374/94‑1 (MQ=60)
                                                                           ACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGA                    <  SRR4302545.1607525/94‑1 (MQ=60)
                                                                           ACCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGA                    <  SRR4302545.10462036/94‑1 (MQ=60)
                                                                            CCACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGAT                   >  SRR4302545.6551786/1‑94 (MQ=60)
                                                                             CACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATA                  >  SRR4302545.233519/1‑94 (MQ=60)
                                                                              CCGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAA                 <  SRR4302545.9995045/94‑1 (MQ=60)
                                                                              CCGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAA                 <  SRR4302545.8797322/94‑1 (MQ=60)
                                                                              ACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAA                 <  SRR4302545.118107/94‑1 (MQ=60)
                                                                              ACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAA                 >  SRR4302545.4197115/1‑94 (MQ=60)
                                                                              ACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAA                 <  SRR4302545.1067125/94‑1 (MQ=60)
                                                                              ACGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAA                 >  SRR4302545.9476990/1‑94 (MQ=60)
                                                                               CGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAG                <  SRR4302545.3429032/94‑1 (MQ=60)
                                                                               CGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAG                <  SRR4302545.1292685/94‑1 (MQ=60)
                                                                               CGGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAG                <  SRR4302545.1531043/94‑1 (MQ=60)
                                                                                GGTAACGCTGGGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGA               <  SRR4302545.8261190/94‑1 (MQ=60)
                                                                                GGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGA               <  SRR4302545.6355806/94‑1 (MQ=60)
                                                                                GGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGA               <  SRR4302545.3389125/94‑1 (MQ=60)
                                                                                GGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGA               >  SRR4302545.3655294/1‑94 (MQ=60)
                                                                                CGTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACAAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGA               <  SRR4302545.6022211/94‑1 (MQ=60)
                                                                                 GTAACGCTGAGCGGATGCGCGATCCGGCAACGGTTAAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAA              <  SRR4302545.10724243/94‑1 (MQ=60)
                                                                                 GTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAA              >  SRR4302545.3751885/1‑94 (MQ=60)
                                                                                 GTAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAA              >  SRR4302545.1098220/1‑94 (MQ=60)
                                                                                  GAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAAC             <  SRR4302545.4857862/94‑1 (MQ=60)
                                                                                  GAACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAAC             <  SRR4302545.6392313/94‑1 (MQ=60)
                                                                                   AACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACG            >  SRR4302545.6145552/1‑94 (MQ=60)
                                                                                   AACGCGGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACG            <  SRR4302545.7831639/94‑1 (MQ=60)
                                                                                    ACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGT           >  SRR4302545.3107365/1‑94 (MQ=60)
                                                                                    ACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGT           <  SRR4302545.959365/94‑1 (MQ=60)
                                                                                    ACGCTGAGCGGATGCGCGATCCGGCAACGGGTCAAGCACACACGGCACACACAAACCTGCCAGTTCTGCTGATTTACGTTGGCGATAAGAACGT           >  SRR4302545.10597872/1‑94 (MQ=60)
                                                                                     CGCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTG          >  SRR4302545.1476448/1‑94 (MQ=60)
                                                                                      GCTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGA         <  SRR4302545.8127840/94‑1 (MQ=60)
                                                                                       CTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACGCGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAA        <  SRR4302545.5285007/94‑1 (MQ=60)
                                                                                       CTGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAA        >  SRR4302545.2130325/1‑94 (MQ=60)
                                                                                        TGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAA       <  SRR4302545.5019534/94‑1 (MQ=60)
                                                                                        TGAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAA       <  SRR4302545.5475957/94‑1 (MQ=60)
                                                                                         GAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTTGTGATAAGAACGTGAAAG      >  SRR4302545.9482050/1‑94 (MQ=60)
                                                                                         GAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAG      <  SRR4302545.8911353/94‑1 (MQ=60)
                                                                                         GAGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAG      >  SRR4302545.10855270/1‑94 (MQ=60)
                                                                                          AGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGC     <  SRR4302545.2163879/94‑1 (MQ=60)
                                                                                          AGCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGC     <  SRR4302545.6395486/94‑1 (MQ=60)
                                                                                           GCGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCG    <  SRR4302545.3405162/94‑1 (MQ=60)
                                                                                            CGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGG   >  SRR4302545.446644/1‑94 (MQ=60)
                                                                                            CGGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGG   >  SRR4302545.7401294/1‑94 (MQ=60)
                                                                                             GGATGCGCTATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGT  <  SRR4302545.4321848/94‑1 (MQ=60)
                                                                                             GGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGT  >  SRR4302545.8386829/1‑94 (MQ=60)
                                                                                             GGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGT  >  SRR4302545.7613846/1‑94 (MQ=60)
                                                                                             |                                                                                             
AAGCGCTGGATCACTGCGTGGAAGAAGTCGCGAAAGCGGTTGAGTCCGTGGGTGGACAACTGCTGATCACCGCTGACCACGGTAACGCTGAGCAGATGCGCGATCCGGCAACGGGTCAGGCACACACGGCACACACCAACCTGCCAGTTCCGCTGATTTACGTTGGTGATAAGAACGTGAAAGCGGT  >  NC_000913/3784142‑3784328

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑