Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	647	270.1	21258240	98.4%	20918108	94.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,936,574	C→T	A241V (GCC→GTC)	rbsR →	RbsR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,936,574	0	C	T	99.5%	2376.2 / ‑4.1	627	A241V (GCC→GTC)	rbsR	RbsR
Reads supporting (aligned to +/- strand): ref base C (1/2); new base T (216/405); total (219/408)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

Only 100 of 687 total aligned reads displayed.

TCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGCCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATCGCGGTGAT  >  NC_000913/3936482‑3936664

 ccTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGGTTGTCGCTATGCGCCACCTGCT‑ATCAAATCCCCTGCGGCCTCCGGTCg                                                                                          >  2:3385282/1‑94 (MQ=255)

  cTGATGGCTATGAAGTCACTGGTAATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGt                                                                                         <  1:7263740/94‑1 (MQ=255)

    gATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCt                                                                                       <  2:2653139/94‑1 (MQ=255)

    gATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGGTTGATGCTATGCGCCCACCCCTCTTCACA‑CCCCCGCCTCCTCAGGTCGTCt                                                                                       >  2:1895169/1‑94 (MQ=255)

      tGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCttt                                                                                     <  2:5857609/94‑1 (MQ=255)

       ggCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGGTTGACGCTATGCGCCCACTGCT‑CTCACATCCCCTGCGTCCTCCGGGCGGCtttt                                                                                    >  1:661741/1‑93 (MQ=255)

         cTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTAcc                                                                                  <  2:660511/94‑1 (MQ=255)

          tATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCg                                                                                 <  1:9539925/94‑1 (MQ=255)

          caTGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCg                                                                                 <  1:8315000/93‑1 (MQ=255)

           aTGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCgg                                                                                <  1:3296565/94‑1 (MQ=255)

              aaGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGaaa                                                                             <  1:5824760/94‑1 (MQ=255)

               aGTCGCTGGTGATTTTGAATTTAACGGCGCGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAAt                                                                            <  2:2452137/94‑1 (MQ=255)

               aGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAAt                                                                            <  2:1274494/94‑1 (MQ=255)

               aGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAAt                                                                            <  1:6862144/94‑1 (MQ=255)

               aGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAAt                                                                            <  1:9993877/94‑1 (MQ=255)

               aGTCACTGAAGATTTTATATTTAACGGCGGGTTAGACGATAAACGCCACCTGCT‑ATCACATCCGCTGAGTCCTCAGGTCGTAAATACCGGagaa                                                                            <  1:9365648/94‑4 (MQ=255)

                 tCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGa                                                                          <  2:6317392/94‑1 (MQ=255)

                 tCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGa                                                                          <  1:7714255/94‑1 (MQ=255)

                 gcACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGa                                                                          <  1:3219005/93‑1 (MQ=255)

                  cACTGGTGATTTTGAATTTAACGGCGGGGTTGTCGCTTTTCGCCAACTGCT‑ATCACATCCGCCGCGCCCCCAGGTCGTCTTTTCCGGAAAAGaa                                                                         >  2:4173364/1‑93 (MQ=255)

                     tGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCt                                                                      <  2:843472/94‑1 (MQ=255)

                     tGGTGATTTTGAATTTAACGGCGGGGTTGTCGCTATGCGCCCACTGCT‑ATCAAATACGCTGCGTCCTCCGGTCTGCTTTTTCTGAAATGACGCt                                                                      >  2:5705529/1‑94 (MQ=255)

                        tGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATg                                                                   <  1:2747232/94‑1 (MQ=255)

                        tGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATg                                                                   <  1:7415883/94‑1 (MQ=255)

                          aTTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGc                                                                 <  2:2018453/94‑1 (MQ=255)

                           ttttGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCt                                                                <  1:4973259/94‑1 (MQ=255)

                           ttttGAATTTAACGGCGGGGTTGTGGCTATGCGCCAACTGCT‑ATCACAACCCCTCCCTCCTCAGGGCGTCTTTTCCGGGAAAGACGCTCTTGCt                                                                >  2:7361419/1‑94 (MQ=255)

                               gAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTg                                                            <  2:2808009/94‑1 (MQ=255)

                                 aTTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGc                                                          <  2:7996534/94‑1 (MQ=255)

                                 aTTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGc                                                          <  2:9142369/94‑1 (MQ=255)

                                  tttAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCg                                                         <  1:8094811/94‑1 (MQ=255)

                                  tttAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCg                                                         <  2:9549610/94‑1 (MQ=255)

                                  tttAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCg                                                         <  2:7672642/94‑1 (MQ=255)

                                    tAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGtt                                                       <  1:8218126/94‑1 (MQ=255)

                                        ggcggGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTAcc                                                   <  2:7099569/94‑1 (MQ=255)

                                            ggTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGc                                               <  1:1981074/94‑1 (MQ=255)

                                            ggTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGc                                               >  1:341563/1‑94 (MQ=255)

                                             gTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCg                                              >  2:5015745/1‑94 (MQ=255)

                                             gTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCg                                              <  2:6127692/94‑1 (MQ=255)

                                             gTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCg                                              >  1:8507161/1‑94 (MQ=255)

                                               ttGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGtt                                            >  1:1356636/1‑94 (MQ=255)

                                                tGACGCTATGCGCCAGCTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTta                                           >  2:8316149/1‑94 (MQ=255)

                                                tGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTta                                           >  2:8692819/1‑94 (MQ=255)

                                                tGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTta                                           >  2:4783011/1‑94 (MQ=255)

                                                tGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTta                                           >  2:1203074/1‑94 (MQ=255)

                                                 gACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTtat                                          >  2:10411860/1‑94 (MQ=255)

                                                   cGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTatat                                        >  2:8672257/1‑94 (MQ=255)

                                                     cTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCa                                      <  1:3736296/94‑1 (MQ=255)

                                                     cTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCa                                      >  2:3980653/1‑94 (MQ=255)

                                                       aTGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAgg                                    <  2:3680902/94‑1 (MQ=255)

                                                         gcgcCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCa                                  <  1:6328411/94‑1 (MQ=255)

                                                          cgcCACCTGCG‑ATGACATCCGGTGCGTCCTAAGGCCGTCTTTACCGGAAATGACGCTATGGCTGTTGTCGTTTACCAGGCGTTATATCAGGCag                                 <  1:8792100/94‑1 (MQ=255)

                                                          cgcCAACTGCT‑ATCACCTCCCCTGCGTCCTGAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGGTTACCAGGCGTTATATCAGGCag                                 <  2:2961836/94‑1 (MQ=255)

                                                          cgcCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATTGCTGTTGGCGTTTACCAGGCGTTATATCAGGCag                                 <  1:1203859/94‑1 (MQ=255)

                                                          cgcCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCag                                 >  1:2466808/1‑94 (MQ=255)

                                                             cAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATTAGGCAGAGt                              >  1:2875611/1‑94 (MQ=255)

                                                             cAACTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGt                              <  2:5482909/94‑1 (MQ=255)

                                                              aaCTGCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGtt                             <  2:8935564/94‑1 (MQ=255)

                                                                  gCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAg                         <  2:9248803/94‑1 (MQ=255)

                                                                  gCT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAg                         <  2:6468097/94‑1 (MQ=255)

                                                                   cT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAgg                        <  1:9155031/94‑1 (MQ=255)

                                                                   cT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAgg                        <  2:5170934/94‑1 (MQ=255)

                                                                   cT‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGAGTTATATCAGGCAGAGTTACAgg                        <  2:4515279/94‑1 (MQ=255)

                                                                    t‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGt                       >  2:8552321/1‑94 (MQ=255)

                                                                    t‑ATCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGt                       <  1:7561724/94‑1 (MQ=255)

                                                                       tCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTc                     >  1:4015436/1‑94 (MQ=255)

                                                                       tCACATCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTc                     <  1:5610439/94‑1 (MQ=255)

                                                                       acccaTCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTc                     <  2:3125301/91‑1 (MQ=255)

                                                                         acaTCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCg                   <  1:4486763/94‑1 (MQ=255)

                                                                          caTCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGc                  <  1:10498601/94‑1 (MQ=255)

                                                                          caTCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGc                  >  2:16975/1‑94 (MQ=255)

                                                                           ctCCGCTGCGTCCTCAGGTAGTCTTTACCGGAAATGACACTATGACTGTTGGCGTTAACCAGGCGTTATATCAAGCAGAATTACAGGTTCCGCa                 <  2:9387551/93‑1 (MQ=255)

                                                                           aTCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCa                 <  2:3881585/94‑1 (MQ=255)

                                                                           aTCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCa                 >  2:10319678/1‑94 (MQ=255)

                                                                           aTCCGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCa                 <  2:6833296/94‑1 (MQ=255)

                                                                             ccGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAgg               <  2:10570855/94‑1 (MQ=255)

                                                                              cGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGa              <  2:9836019/94‑1 (MQ=255)

                                                                              cGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGa              >  2:1575084/1‑94 (MQ=255)

                                                                              cGCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGa              >  1:4740221/1‑94 (MQ=255)

                                                                              cGCTGCGTACTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGa              >  1:10391476/1‑94 (MQ=255)

                                                                               gCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGat             >  1:10023695/1‑94 (MQ=255)

                                                                               gCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGat             <  1:9798251/94‑1 (MQ=255)

                                                                               gCTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGat             <  2:4357013/94‑1 (MQ=255)

                                                                               gCTGCGTCCTCAGGCCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGat             <  1:9003616/94‑1 (MQ=255)

                                                                                cTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGata            <  1:1067658/94‑1 (MQ=255)

                                                                                cTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGata            >  1:1500784/1‑94 (MQ=255)

                                                                                cTGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGata            <  1:4829359/94‑1 (MQ=255)

                                                                                 tGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGatat           >  2:775351/1‑94 (MQ=255)

                                                                                 tGCGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGatat           <  1:3089999/94‑1 (MQ=255)

                                                                                   cGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATcg         <  1:6475238/94‑1 (MQ=255)

                                                                                   cGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATcg         >  1:2219423/1‑94 (MQ=255)

                                                                                   cGTCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTAGATCAGGCAGAGTTACAGGTTCCGCAGGATATcg         >  2:10179173/1‑94 (MQ=255)

                                                                                     tCCTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGTCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATcgcg       <  1:5225521/94‑1 (MQ=255)

                                                                                      ccTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATCGCgg      >  1:6684239/1‑94 (MQ=255)

                                                                                       cTCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATCGCGGt     <  1:172945/94‑1 (MQ=255)

                                                                                        tCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATCGCGGTg    <  1:4196546/94‑1 (MQ=255)

                                                                                        tCAGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATCGCGGTg    <  1:4617589/94‑1 (MQ=255)

                                                                                         cAGGTCGTCTTTACCGGAAATGATGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATCGCGGTGa   <  2:2226361/94‑1 (MQ=255)

                                                                                          aGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATCGCGGTGAt  >  2:7519139/1‑94 (MQ=255)

                                                                                          aGGTCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATCGCGGTGAt  <  1:7126791/94‑1 (MQ=255)

TCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCT‑ATCACATCCGCTGCGTCCTCAGGCCGTCTTTACCGGAAATGACGCTATGGCTGTTGGCGTTTACCAGGCGTTATATCAGGCAGAGTTACAGGTTCCGCAGGATATCGCGGTGAT  >  NC_000913/3936482‑3936664

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 392 total aligned reads displayed.

TCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAG  >  NC_000913/3938494‑3938680

TCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGC                                                                                               >  SRR4302545.5572824/1‑94 (MQ=60)

TCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGC                                                                                               >  SRR4302545.13964/1‑94 (MQ=60)

TCCAGCGTTCACGGCGAAAGTTCTATTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGC                                                                                               >  SRR4302545.9236663/1‑94 (MQ=60)

 CCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCT                                                                                              <  SRR4302545.491242/94‑1 (MQ=60)

 CCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCT                                                                                              <  SRR4302545.5747908/94‑1 (MQ=60)

 CCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCT                                                                                              <  SRR4302545.2517067/94‑1 (MQ=60)

 CCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCT                                                                                              <  SRR4302545.2440925/94‑1 (MQ=60)

 CCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCT                                                                                              <  SRR4302545.10418217/94‑1 (MQ=60)

 CCAGCGTTCACGGCGAAAGTACTCTTTGAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCT                                                                                              <  SRR4302545.5967206/94‑1 (MQ=60)

  CAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCCACCGT‑‑‑TGCtc                                                                                             >  SRR4302545.8224976/1‑92 (MQ=60)

  CAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCtg                                                                                             >  SRR4302545.8422414/1‑92 (MQ=60)

   AGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCtgc                                                                                            >  SRR4302545.10571822/1‑91 (MQ=60)

   AGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCtgc                                                                                            >  SRR4302545.10830032/1‑91 (MQ=60)

   AGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCtgc                                                                                            <  SRR4302545.4726005/94‑4 (MQ=60)

     CGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCtgcca                                                                                          >  SRR4302545.5889059/1‑89 (MQ=60)

      GTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCtgccat                                                                                         >  SRR4302545.848728/1‑88 (MQ=60)

      GTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCtgccag                                                                                         >  SRR4302545.10631153/1‑88 (MQ=60)

      GTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCtgccag                                                                                         >  SRR4302545.5349205/1‑88 (MQ=60)

      GTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCtgccag                                                                                         >  SRR4302545.2872990/1‑88 (MQ=60)

      GTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCtgccag                                                                                         >  SRR4302545.3166865/1‑88 (MQ=60)

        TCCCGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTA                                                                                          <  SRR4302545.2707526/94‑1 (MQ=60)

         CACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAG                                                                                         <  SRR4302545.4643657/94‑1 (MQ=60)

         CACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAG                                                                                         <  SRR4302545.7592995/94‑1 (MQ=60)

         CACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAG                                                                                         >  SRR4302545.321082/1‑94 (MQ=60)

         CACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTCTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAG                                                                                         <  SRR4302545.9252942/94‑1 (MQ=60)

          ACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGG                                                                                        >  SRR4302545.5432117/1‑94 (MQ=60)

            GGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCA                                                                                      >  SRR4302545.8117377/1‑94 (MQ=60)

            GGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCA                                                                                      >  SRR4302545.5266120/1‑94 (MQ=60)

             GCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAG                                                                                     <  SRR4302545.9413423/94‑1 (MQ=60)

             GCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAG                                                                                     <  SRR4302545.8109459/94‑1 (MQ=60)

             GCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAG                                                                                     <  SRR4302545.5874912/94‑1 (MQ=60)

              CTAAAGTGCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGG                                                                                    <  SRR4302545.4484747/94‑1 (MQ=60)

              CGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGG                                                                                    >  SRR4302545.190006/1‑94 (MQ=60)

              CGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGG                                                                                    <  SRR4302545.7676866/94‑1 (MQ=60)

              CGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGG                                                                                    >  SRR4302545.3680293/1‑94 (MQ=60)

              CGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGG                                                                                    >  SRR4302545.4087611/1‑94 (MQ=60)

              CGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGG                                                                                    >  SRR4302545.9398435/1‑94 (MQ=60)

              CGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGG                                                                                    >  SRR4302545.1697274/1‑94 (MQ=60)

               GAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGC                                                                                   >  SRR4302545.6966994/1‑94 (MQ=60)

               GAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGC                                                                                   >  SRR4302545.1740933/1‑94 (MQ=60)

               GAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGC                                                                                   >  SRR4302545.6912823/1‑94 (MQ=60)

               GAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGC                                                                                   >  SRR4302545.1373657/1‑94 (MQ=60)

               GAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGC                                                                                   >  SRR4302545.5788442/1‑94 (MQ=60)

               GAAAGTTCTCTTTTAATACCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGC                                                                                   >  SRR4302545.10334843/1‑94 (MQ=60)

                AAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCT                                                                                  >  SRR4302545.7719955/1‑94 (MQ=60)

                 AAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTT                                                                                 <  SRR4302545.6453796/94‑1 (MQ=60)

                 AAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTT                                                                                 <  SRR4302545.4723715/94‑1 (MQ=60)

                 AAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTT                                                                                 >  SRR4302545.7781565/1‑94 (MQ=60)

                  AGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGAGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTG                                                                                <  SRR4302545.9051587/94‑1 (MQ=60)

                  AGTGCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTG                                                                                <  SRR4302545.3832396/94‑1 (MQ=60)

                   GTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGG                                                                               >  SRR4302545.3621192/1‑94 (MQ=60)

                   GTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGC                                                                               <  SRR4302545.4326094/94‑1 (MQ=60)

                   GTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGC                                                                               <  SRR4302545.2794524/94‑1 (MQ=60)

                   GTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGC                                                                               <  SRR4302545.485221/94‑1 (MQ=60)

                   GTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGC                                                                               <  SRR4302545.4857496/94‑1 (MQ=60)

                    TTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCG                                                                              >  SRR4302545.10906810/1‑94 (MQ=60)

                    TTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCG                                                                              <  SRR4302545.5126288/94‑1 (MQ=60)

                      CTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGC                                                                            <  SRR4302545.3775710/94‑1 (MQ=60)

                       TCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCT                                                                           >  SRR4302545.3846690/1‑94 (MQ=60)

                       TCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCT                                                                           <  SRR4302545.8889320/94‑1 (MQ=60)

                        tgTTTAATGCCGCCATTTCGGTCATTAACGTATTGAGATGAGCTTTCTGTTCCGCGGTCCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTC                                                                          <  SRR4302545.10679753/92‑1 (MQ=60)

                        CTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTC                                                                          >  SRR4302545.10599393/1‑94 (MQ=60)

                        CTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTC                                                                          >  SRR4302545.9891059/1‑94 (MQ=60)

                         TTTTAATGCCGCCATTTCGTCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCC                                                                         <  SRR4302545.10242225/94‑1 (MQ=60)

                          TTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCA                                                                        >  SRR4302545.10263091/1‑94 (MQ=60)

                           TTAATGCCGCCATTTCGGCTATTAACGTATTGAGATGGGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAG                                                                       <  SRR4302545.7574870/94‑1 (MQ=60)

                           TTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAG                                                                       <  SRR4302545.8686559/94‑1 (MQ=60)

                           TTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAG                                                                       <  SRR4302545.10535815/94‑1 (MQ=60)

                            TAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGA                                                                      <  SRR4302545.4009313/94‑1 (MQ=60)

                             AATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCCGAC                                                                     >  SRR4302545.8634207/1‑94 (MQ=60)

                               TGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAG                                                                   <  SRR4302545.5664079/94‑1 (MQ=60)

                                GCCGCTGTTTCGTCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGA                                                                  <  SRR4302545.71067/94‑1 (MQ=60)

                                GCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGA                                                                  <  SRR4302545.46795/94‑1 (MQ=60)

                                GCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGA                                                                  <  SRR4302545.9536005/94‑1 (MQ=60)

                                  cgcacTTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATA                                                                <  SRR4302545.6473788/89‑1 (MQ=60)

                                  CGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATA                                                                <  SRR4302545.10870178/94‑1 (MQ=60)

                                  CGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATA                                                                <  SRR4302545.10219889/94‑1 (MQ=60)

                                  CGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATA                                                                >  SRR4302545.2682798/1‑94 (MQ=60)

                                   GCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTCGGGCTCACGACAGATAC                                                               >  SRR4302545.3542779/1‑94 (MQ=60)

                                   CCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATAC                                                               <  SRR4302545.1365707/94‑1 (MQ=60)

                                      ATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCA                                                            >  SRR4302545.5052016/1‑94 (MQ=60)

                                      ATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCA                                                            >  SRR4302545.9166376/1‑94 (MQ=60)

                                          CGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAAC                                                        >  SRR4302545.2331390/1‑94 (MQ=60)

                                           GGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACC                                                       <  SRR4302545.354920/94‑1 (MQ=60)

                                            GCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCA                                                      >  SRR4302545.3565716/1‑94 (MQ=60)

                                             CCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAG                                                     >  SRR4302545.1182360/1‑94 (MQ=60)

                                             CCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAG                                                     <  SRR4302545.1494775/94‑1 (MQ=60)

                                              CATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGA                                                    >  SRR4302545.10003327/1‑94 (MQ=60)

                                              CATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGA                                                    >  SRR4302545.10867603/1‑94 (MQ=60)

                                               ATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAA                                                   >  SRR4302545.8735687/1‑94 (MQ=60)

                                                  AACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGT                                                <  SRR4302545.339293/94‑1 (MQ=60)

                                                  AACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGT                                                >  SRR4302545.5534439/1‑94 (MQ=60)

                                                  AACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGT                                                <  SRR4302545.5242191/94‑1 (MQ=60)

                                                  AACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAAACAGAAAGT                                                >  SRR4302545.4059607/1‑94 (MQ=60)

                                                   tctctgatctATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTG                                               <  SRR4302545.5098205/84‑1 (MQ=60)

                                                   ACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTG                                               <  SRR4302545.1782746/94‑1 (MQ=60)

                                                    CGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGA                                              >  SRR4302545.2532586/1‑94 (MQ=60)

                                                    CGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGA                                              >  SRR4302545.1080956/1‑94 (MQ=60)

                                                    CGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGA                                              >  SRR4302545.2966159/1‑94 (MQ=60)

                                                    CGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAAAAAGTGA                                              >  SRR4302545.8166266/1‑94 (MQ=60)

                                                     GTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGAT                                             <  SRR4302545.8741328/94‑1 (MQ=60)

                                                     GTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGAT                                             <  SRR4302545.2658357/94‑1 (MQ=60)

                                                      TATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATCACCAGAAAGTGATC                                            >  SRR4302545.7481848/1‑94 (MQ=60)

                                                      TATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATC                                            >  SRR4302545.79074/1‑94 (MQ=60)

                                                      TATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATC                                            <  SRR4302545.9993430/94‑1 (MQ=60)

                                                       ATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGCAAGTGATCG                                           >  SRR4302545.7359240/1‑94 (MQ=60)

                                                       ATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCG                                           <  SRR4302545.5125474/94‑1 (MQ=60)

                                                       ATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCG                                           >  SRR4302545.9445009/1‑94 (MQ=60)

                                                       ATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCG                                           >  SRR4302545.7565062/1‑94 (MQ=60)

                                                       ATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCG                                           <  SRR4302545.7300268/94‑1 (MQ=60)

                                                       ATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCA                                           <  SRR4302545.4825346/94‑1 (MQ=60)

                                                       ATTGAGATGCACTTTCTGTTCCGGGGTGCCACCCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCG                                           <  SRR4302545.97933/94‑1 (MQ=60)

                                                          GAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATG                                        <  SRR4302545.9469789/94‑1 (MQ=60)

                                                          GAGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATG                                        <  SRR4302545.2427242/94‑1 (MQ=60)

                                                           AGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGA                                       <  SRR4302545.3318583/94‑1 (MQ=60)

                                                           AGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGA                                       >  SRR4302545.5389022/1‑94 (MQ=60)

                                                           AGATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGA                                       >  SRR4302545.3665650/1‑94 (MQ=60)

                                                            GATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGAC                                      <  SRR4302545.2716449/94‑1 (MQ=60)

                                                            GATGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGAC                                      <  SRR4302545.9708329/94‑1 (MQ=60)

                                                              TGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCT                                    <  SRR4302545.835622/94‑1 (MQ=60)

                                                              TGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCT                                    <  SRR4302545.3407425/94‑1 (MQ=60)

                                                              TGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCT                                    <  SRR4302545.7369783/94‑1 (MQ=60)

                                                              TGCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCT                                    <  SRR4302545.10744157/94‑1 (MQ=60)

                                                              TGCGCTTGCTGGTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGTGGCTCCAGACAGATACGCATAACTAGATAGTGATCGATGACCT                                    >  SRR4302545.9616200/1‑94 (MQ=60)

                                                               GCGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATTACCAGAAAATGATCGCTGACCTG                                   >  SRR4302545.4264387/1‑94 (MQ=60)

                                                                CGCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGA                                  <  SRR4302545.5945287/94‑1 (MQ=60)

                                                                CGATTTCTGTTCCGCGGTTCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGA                                  <  SRR4302545.1883992/94‑1 (MQ=60)

                                                                 GCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGAT                                 <  SRR4302545.227616/94‑1 (MQ=60)

                                                                 GCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGAT                                 <  SRR4302545.7440758/94‑1 (MQ=60)

                                                                 GCTTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGAT                                 >  SRR4302545.1851946/1‑94 (MQ=60)

                                                                   TTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGA                               >  SRR4302545.8461169/1‑94 (MQ=60)

                                                                   GTTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGA                               <  SRR4302545.8915402/94‑1 (MQ=60)

                                                                    TTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAA                              <  SRR4302545.9077054/94‑1 (MQ=60)

                                                                    TTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAA                              <  SRR4302545.6430947/94‑1 (MQ=60)

                                                                    TTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAA                              <  SRR4302545.6035758/94‑1 (MQ=60)

                                                                    TTCTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCAGCTCCAGACTGATACGCATGACCATAAAGTGATCGATGAacttgtgaa                              >  SRR4302545.97323/1‑85 (MQ=60)

                                                                      CTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAG                            <  SRR4302545.1017858/94‑1 (MQ=60)

                                                                      CTGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGCGATCGATGACCTGATGAAAG                            >  SRR4302545.2915763/1‑94 (MQ=60)

                                                                       TGTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGT                           <  SRR4302545.7307678/94‑1 (MQ=60)

                                                                        GTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTT                          <  SRR4302545.8627989/94‑1 (MQ=60)

                                                                        GTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTT                          <  SRR4302545.6340960/94‑1 (MQ=60)

                                                                        GTTCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTT                          >  SRR4302545.282431/1‑94 (MQ=60)

                                                                         TCCCGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTC                         <  SRR4302545.8710406/94‑1 (MQ=60)

                                                                           CCGCGGTGCGACCCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTC                       <  SRR4302545.4314871/94‑1 (MQ=60)

                                                                            CGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCT                      >  SRR4302545.5339802/1‑94 (MQ=60)

                                                                            CGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCT                      <  SRR4302545.2795474/94‑1 (MQ=60)

                                                                            CGCGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCT                      <  SRR4302545.10406986/94‑1 (MQ=60)

                                                                             GCGTTGCCAACCGTGTCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTT                     <  SRR4302545.5824338/94‑1 (MQ=60)

                                                                              CGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTC                    <  SRR4302545.7222100/94‑1 (MQ=60)

                                                                              CGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTC                    <  SRR4302545.1366763/94‑1 (MQ=60)

                                                                              CGGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTC                    <  SRR4302545.8870616/94‑1 (MQ=60)

                                                                               GGTGCCACCCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCT                   <  SRR4302545.2611942/94‑1 (MQ=60)

                                                                               GGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAGGTTCTCTTCT                   >  SRR4302545.10476720/1‑94 (MQ=60)

                                                                               GGTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCT                   >  SRR4302545.7205368/1‑94 (MQ=60)

                                                                               GGAGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCT                   <  SRR4302545.7127543/94‑1 (MQ=60)

                                                                                GTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTG                  >  SRR4302545.6600627/1‑94 (MQ=60)

                                                                                GTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTG                  <  SRR4302545.4283934/94‑1 (MQ=60)

                                                                                GTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTG                  >  SRR4302545.8816173/1‑94 (MQ=60)

                                                                                GTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTG                  <  SRR4302545.2378511/94‑1 (MQ=60)

                                                                                GTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTG                  >  SRR4302545.5710210/1‑94 (MQ=60)

                                                                                GTGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTG                  <  SRR4302545.9012796/94‑1 (MQ=60)

                                                                                 ggccatcgtttgcgGCCAGTAGGATGGCTTGCGTCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAGGTTCTCTGCTGT                 <  SRR4302545.5984641/80‑1 (MQ=60)

                                                                                 TGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGT                 <  SRR4302545.3866618/94‑1 (MQ=60)

                                                                                 TGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGT                 <  SRR4302545.1442090/94‑1 (MQ=60)

                                                                                 TGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGT                 >  SRR4302545.3677550/1‑94 (MQ=60)

                                                                                 TGCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGT                 >  SRR4302545.481878/1‑94 (MQ=60)

                                                                                  GCCAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAATTCTCTTCTGTC                >  SRR4302545.10115827/1‑94 (MQ=60)

                                                                                    CAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCAT              <  SRR4302545.2356019/94‑1 (MQ=60)

                                                                                    CAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCAT              >  SRR4302545.7704994/1‑94 (MQ=60)

                                                                                    CAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCAT              >  SRR4302545.2068610/1‑94 (MQ=60)

                                                                                    CAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCAT              >  SRR4302545.4519882/1‑94 (MQ=60)

                                                                                    CAACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCAT              <  SRR4302545.6712614/94‑1 (MQ=60)

                                                                                     AACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATC             >  SRR4302545.10008593/1‑94 (MQ=60)

                                                                                     AACCGTTGCTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATC             >  SRR4302545.3890174/1‑94 (MQ=60)

                                                                                       ccgttgcTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCA           >  SRR4302545.9807310/8‑94 (MQ=60)

                                                                                       ccgttgcTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCA           <  SRR4302545.3857301/87‑1 (MQ=60)

                                                                                       ccgttgcTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCA           >  SRR4302545.5008159/8‑94 (MQ=60)

                                                                                        cgttgcTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCAC          >  SRR4302545.4565901/7‑94 (MQ=60)

                                                                                        cgttgcTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCAC          >  SRR4302545.5213982/7‑94 (MQ=60)

                                                                                        cgttgcTGCCAGCAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCAC          >  SRR4302545.1073774/7‑94 (MQ=60)

                                                                                         gttgcTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAGGTTCTCTTCTGTCATCCACC         >  SRR4302545.5490312/6‑94 (MQ=60)

                                                                                         gttgcTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACC         <  SRR4302545.551512/89‑1 (MQ=60)

                                                                                         gttgcTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACC         <  SRR4302545.6773391/89‑1 (MQ=60)

                                                                                         GC‑‑‑TGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGG      <  SRR4302545.6188239/94‑1 (MQ=60)

                                                                                         GC‑‑‑TGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGG      <  SRR4302545.6777503/94‑1 (MQ=60)

                                                                                         GC‑‑‑TGCCAGTAGGCAGGCTTGCGGCTCCAGACAGAGACGCATAACCAGAAAGTGATCGAGGACCTGATGAAAGTTCTCTTCTGTCATCCCCCAGG      <  SRR4302545.1441602/94‑1 (MQ=60)

                                                                                          ttgcTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCA        <  SRR4302545.2300359/90‑1 (MQ=60)

                                                                                          gtgcTGCCAGTAGGCAGGGTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCA        <  SRR4302545.200004/90‑1 (MQ=60)

                                                                                          C‑‑‑TGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGT     >  SRR4302545.8307257/1‑94 (MQ=60)

                                                                                           tgcTGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAG       <  SRR4302545.3012586/91‑1 (MQ=60)

                                                                                              TGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAGGTTCTCTTCTGTCATCCACCAGGTA    <  SRR4302545.9706430/94‑1 (MQ=60)

                                                                                              TGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTA    <  SRR4302545.3286852/94‑1 (MQ=60)

                                                                                              TGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTA    <  SRR4302545.818366/94‑1 (MQ=60)

                                                                                              TGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTA    <  SRR4302545.2622221/94‑1 (MQ=60)

                                                                                               GCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAA   <  SRR4302545.8572200/94‑1 (MQ=60)

                                                                                               GCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAA   <  SRR4302545.1125852/94‑1 (MQ=60)

                                                                                               GCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAA   >  SRR4302545.1554310/1‑94 (MQ=60)

                                                                                                CCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGCTGAAAGTTCTCTTCTGTCATCCACCAGGTAAG  >  SRR4302545.3068910/1‑94 (MQ=60)

                                                                                                CCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAG  <  SRR4302545.7069645/94‑1 (MQ=60)

                                                                                                CCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAG  <  SRR4302545.2459013/94‑1 (MQ=60)

TCCAGCGTTCACGGCGAAAGTTCTCTTTTAATGCCGCCATTTCGGCCATTAACGTATTGAGATGCGCTTTCTGTTCCGCGGTGCCAACCGT‑‑‑TGCCAGTAGGCAGGCTTGCGGCTCCAGACAGATACGCATAACCAGAAAGTGATCGATGACCTGATGAAAGTTCTCTTCTGTCATCCACCAGGTAAG  >  NC_000913/3938494‑3938680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`