Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,938,905 A→G intergenic (‑99/‑526) yiePs ← / → rrsC hypothetical protein/16S ribosomal RNA

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,938,9050AG96.2% 782.1 / 11.0 240intergenic (‑99/‑526)yiePs/rrsChypothetical protein/16S ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base A (1/8);  new base G (91/140);  total (92/148)
Fisher's exact test for biased strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 242 total aligned reads displayed.

TGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACC  >  NC_000913/3938814‑3938994
                                                                                           |                                                                                         
    cGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGaaa                                                                                      <  1:5974350/93‑1 (MQ=255)
     gCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGCTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAg                                                                                     >  2:1548741/1‑93 (MQ=255)
      cccAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAgg                                                                                    <  1:3959341/93‑1 (MQ=255)
      cccAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCAGATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAgg                                                                                    <  2:2074258/93‑1 (MQ=255)
       ccAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGt                                                                                   <  2:1433091/93‑1 (MQ=255)
        cAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGtt                                                                                  <  1:1039862/93‑1 (MQ=255)
        cAGCTCAATCTCACCGCGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGtt                                                                                  >  2:3171939/1‑93 (MQ=255)
         aGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGttt                                                                                 <  1:5119502/93‑1 (MQ=255)
           cTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGttttt                                                                               >  1:1463143/1‑93 (MQ=255)
            tCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTc                                                                              <  2:5439886/93‑1 (MQ=255)
              aaTCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCtg                                                                            <  2:5940824/93‑1 (MQ=255)
              aaTCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCtg                                                                            <  2:5617222/93‑1 (MQ=255)
               aTCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCtgt                                                                           <  2:2987785/93‑1 (MQ=255)
               aTCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCtgt                                                                           >  1:1178244/1‑93 (MQ=255)
               aTCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGGTTTTCtgt                                                                           >  2:121296/1‑93 (MQ=255)
                 ctcACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGc                                                                         >  1:3091037/1‑93 (MQ=255)
                  tcACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCa                                                                        <  2:1168008/93‑1 (MQ=255)
                    aCCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGc                                                                      >  2:5382088/1‑93 (MQ=255)
                     ccGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCt                                                                     <  2:747737/93‑1 (MQ=255)
                     ccGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCt                                                                     >  1:4383288/1‑93 (MQ=255)
                      cGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTa                                                                    >  1:3435398/1‑93 (MQ=255)
                       gggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTaa                                                                   >  1:3585669/1‑93 (MQ=255)
                       gggCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTaa                                                                   <  2:1806328/93‑1 (MQ=255)
                        ggCAAAATGTTGCCGGGTTCATATTCACCTTTTAAGATCGGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAAc                                                                  <  2:5001738/93‑1 (MQ=255)
                           aaaaTGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACtgt                                                               >  1:1110601/1‑93 (MQ=255)
                             aaTGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACtgttg                                                             >  2:6382850/1‑93 (MQ=255)
                             aaTGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACtgttg                                                             <  1:251113/93‑1 (MQ=255)
                               tGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTtgtg                                                           <  1:528716/93‑1 (MQ=255)
                                ggTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTgc                                                          <  1:4120347/93‑1 (MQ=255)
                                ggTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTgc                                                          <  1:725601/93‑1 (MQ=255)
                                     cGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGCTTTTCTGTGCAGCTAACTGTTGTGCGCTTa                                                     >  1:969108/1‑93 (MQ=255)
                                      gggTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTaa                                                    >  2:473447/1‑93 (MQ=255)
                                       ggTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTaaa                                                   <  1:3294744/93‑1 (MQ=255)
                                        gTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAg                                                  <  2:673413/93‑1 (MQ=255)
                                         ttCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAgg                                                 >  1:315274/1‑93 (MQ=255)
                                           cATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTCAAGGCa                                               >  2:1041632/1‑93 (MQ=255)
                                           cATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCa                                               <  1:1869822/93‑1 (MQ=255)
                                            atatTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAt                                              >  2:611758/1‑93 (MQ=255)
                                            atatTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAt                                              >  1:890205/1‑93 (MQ=255)
                                            atatTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAt                                              >  1:4905333/1‑93 (MQ=255)
                                             tatTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAtt                                             >  1:2716915/1‑93 (MQ=255)
                                               ttCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTAc                                           <  2:1751064/93‑1 (MQ=255)
                                                tCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACt                                          <  1:1749196/93‑1 (MQ=255)
                                                tCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACt                                          >  2:3932649/1‑93 (MQ=255)
                                                 cACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACtt                                         <  1:5641424/93‑1 (MQ=255)
                                                 cACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACtt                                         <  2:3522347/93‑1 (MQ=255)
                                                 cACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACtt                                         >  2:5245152/1‑93 (MQ=255)
                                                 cACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACtg                                         <  2:6208519/93‑2 (MQ=255)
                                                  aCCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTa                                        <  2:2678184/93‑1 (MQ=255)
                                                  aCCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTa                                        >  1:4341522/1‑93 (MQ=255)
                                                  aCCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTa                                        >  2:4477507/1‑93 (MQ=255)
                                                   ccTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTAt                                       <  2:831096/93‑1 (MQ=255)
                                                   ccTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTAt                                       <  1:1950478/93‑1 (MQ=255)
                                                    cTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATc                                      <  2:5354083/93‑1 (MQ=255)
                                                     ttttAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCt                                     <  2:3719144/93‑1 (MQ=255)
                                                       ttAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTc                                   >  2:2419257/1‑93 (MQ=255)
                                                         aaGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCct                                 >  1:2883637/1‑93 (MQ=255)
                                                         aaGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCct                                 <  1:635042/93‑1 (MQ=255)
                                                         aaGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCct                                 <  1:6109543/93‑1 (MQ=255)
                                                          aGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctt                                <  1:3202299/93‑1 (MQ=255)
                                                          aGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctt                                <  2:2929461/93‑1 (MQ=255)
                                                           gATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCcttt                               >  1:5768568/1‑93 (MQ=255)
                                                            aTCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttt                              >  1:3706818/1‑93 (MQ=255)
                                                            aTCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttt                              <  2:2504155/93‑1 (MQ=255)
                                                              ccGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGTAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttttc                            <  2:1347615/93‑1 (MQ=255)
                                                              ccGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttttc                            <  1:5066297/93‑1 (MQ=255)
                                                                 ttGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttttcttt                         <  1:1314977/93‑1 (MQ=255)
                                                                 ttGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttttcttt                         <  2:3575768/93‑1 (MQ=255)
                                                                  tGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttttctttt                        <  2:16229/93‑1 (MQ=255)
                                                                  tGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttttctttt                        <  1:6478606/93‑1 (MQ=255)
                                                                   gcgcCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttttcttttt                       >  2:5551535/1‑93 (MQ=255)
                                                                   gcgcCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCctttttcttttt                       <  1:4641365/93‑1 (MQ=255)
                                                                    cgcCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTa                      <  2:4562805/93‑1 (MQ=255)
                                                                    cgcCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTa                      <  1:4671586/93‑1 (MQ=255)
                                                                     gcCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAt                     >  2:3259784/1‑93 (MQ=255)
                                                                     gcCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAt                     <  2:4314508/93‑1 (MQ=255)
                                                                      ccAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTtttatttttatt                    <  2:6298669/93‑1 (MQ=255)
                                                                      ccAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAtt                    >  1:1343998/1‑93 (MQ=255)
                                                                        aGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATtcc                  <  1:5228322/93‑1 (MQ=255)
                                                                           ttCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATtcctcc               <  1:403730/93‑1 (MQ=255)
                                                                            tctcAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTcctcct              <  2:3494280/93‑1 (MQ=255)
                                                                            tctcAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTcctcct              <  1:6392337/93‑1 (MQ=255)
                                                                              tcAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTa            <  1:3858044/93‑1 (MQ=255)
                                                                              tcAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTa            <  1:3980624/93‑1 (MQ=255)
                                                                              tcAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTa            <  2:1962975/93‑1 (MQ=255)
                                                                                aGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGt          >  1:3128116/1‑93 (MQ=255)
                                                                                aGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGt          <  1:160346/93‑1 (MQ=255)
                                                                                 gcgaGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTGCTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTa         <  2:1775463/90‑1 (MQ=255)
                                                                                 gCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTa         <  1:5869886/93‑1 (MQ=255)
                                                                                  ccAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTAt        <  2:2854305/93‑1 (MQ=255)
                                                                                    aGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGc      <  2:3425200/93‑1 (MQ=255)
                                                                                    aGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGc      >  1:59642/1‑93 (MQ=255)
                                                                                    aGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGc      >  2:5774145/1‑93 (MQ=255)
                                                                                    aGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGc      >  1:2344602/1‑93 (MQ=255)
                                                                                    aGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGc      <  2:4091200/93‑1 (MQ=255)
                                                                                     gAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGcc     <  1:5896293/93‑1 (MQ=255)
                                                                                     gAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGcc     <  1:2173156/93‑1 (MQ=255)
                                                                                     gAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGcc     >  2:1569989/1‑93 (MQ=255)
                                                                                     gAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGcc     >  1:5808660/1‑93 (MQ=255)
                                                                                       aCATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGccac   >  1:2256197/1‑93 (MQ=255)
                                                                                           |                                                                                         
TGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACC  >  NC_000913/3938814‑3938994

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGG  >  NC_000913/3938813‑3938997
                                                                                            |                                                                                            
TTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGC                                                                                             >  SRR4302537.6129723/1‑94 (MQ=60)
 TGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCG                                                                                            <  SRR4302537.5253125/94‑1 (MQ=60)
  GCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGA                                                                                           <  SRR4302537.5961122/94‑1 (MQ=60)
     CGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAG                                                                                        <  SRR4302537.6015360/94‑1 (MQ=60)
       CCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGT                                                                                      <  SRR4302537.3987044/94‑1 (MQ=60)
       CCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGT                                                                                      <  SRR4302537.4954578/94‑1 (MQ=60)
        caagttaaatcccgcgGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTT                                                                                     <  SRR4302537.4030017/78‑1 (MQ=60)
         CAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTT                                                                                    <  SRR4302537.1047018/94‑1 (MQ=60)
         CAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTT                                                                                    <  SRR4302537.5174070/94‑1 (MQ=60)
         CAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTT                                                                                    >  SRR4302537.1437915/1‑94 (MQ=60)
          AGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTT                                                                                   <  SRR4302537.5154784/94‑1 (MQ=60)
           GCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTT                                                                                  >  SRR4302537.1473321/1‑94 (MQ=60)
           GCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTT                                                                                  >  SRR4302537.2517985/1‑94 (MQ=60)
             TCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCT                                                                                >  SRR4302537.4799624/1‑94 (MQ=60)
              CAATCTCACCGGGCAAAATGGCGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTG                                                                               >  SRR4302537.3136207/1‑94 (MQ=60)
               AATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGT                                                                              >  SRR4302537.1186437/1‑94 (MQ=60)
                ATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTG                                                                             >  SRR4302537.4003121/1‑94 (MQ=60)
                 TCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGC                                                                            >  SRR4302537.3112597/1‑94 (MQ=60)
                   TCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAG                                                                          <  SRR4302537.4517884/94‑1 (MQ=60)
                     ACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCT                                                                        <  SRR4302537.5953746/94‑1 (MQ=60)
                     ACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCT                                                                        >  SRR4302537.4413537/1‑94 (MQ=60)
                      CCGGGCAAAATGGTGCGGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTA                                                                       <  SRR4302537.5906261/94‑1 (MQ=60)
                      CCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTA                                                                       >  SRR4302537.3459714/1‑94 (MQ=60)
                       CGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAA                                                                      >  SRR4302537.2224760/1‑94 (MQ=60)
                       CGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAA                                                                      >  SRR4302537.3610944/1‑94 (MQ=60)
                        GGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAAC                                                                     <  SRR4302537.2281161/94‑1 (MQ=60)
                        GGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAAC                                                                     <  SRR4302537.1863421/94‑1 (MQ=60)
                         GGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACT                                                                    <  SRR4302537.6358907/94‑1 (MQ=60)
                          GCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTG                                                                   <  SRR4302537.1255147/94‑1 (MQ=60)
                           CAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGT                                                                  >  SRR4302537.1118273/1‑94 (MQ=60)
                            AAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTT                                                                 <  SRR4302537.3724942/94‑1 (MQ=60)
                              AATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGT                                                               <  SRR4302537.252898/94‑1 (MQ=60)
                              AATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGT                                                               >  SRR4302537.856561/1‑94 (MQ=60)
                               ATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTG                                                              <  SRR4302537.3778501/94‑1 (MQ=60)
                               ATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTG                                                              <  SRR4302537.1350891/94‑1 (MQ=60)
                               ATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTG                                                              <  SRR4302537.688666/94‑1 (MQ=60)
                                TGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGC                                                             <  SRR4302537.92476/94‑1 (MQ=60)
                                TGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGC                                                             <  SRR4302537.532342/94‑1 (MQ=60)
                                 GGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCG                                                            <  SRR4302537.1065441/94‑1 (MQ=60)
                                 GGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCG                                                            <  SRR4302537.4149118/94‑1 (MQ=60)
                                 GGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCG                                                            <  SRR4302537.730553/94‑1 (MQ=60)
                                 GGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCG                                                            <  SRR4302537.3177749/94‑1 (MQ=60)
                                  GTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGGTTTTCTGTGCAGCTAACTGTTGTGCGC                                                           >  SRR4302537.5069475/1‑94 (MQ=60)
                                   TGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCT                                                          <  SRR4302537.1270029/94‑1 (MQ=60)
                                     CCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGCTTTTCTGTGCAGCTAACTGTTGTGCGCTTA                                                        >  SRR4302537.975789/1‑94 (MQ=60)
                                      CGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAA                                                       <  SRR4302537.5738566/94‑1 (MQ=60)
                                      CGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAA                                                       >  SRR4302537.2900754/1‑94 (MQ=60)
                                       GGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAA                                                      >  SRR4302537.3149229/1‑94 (MQ=60)
                                        GGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAG                                                     <  SRR4302537.6364028/94‑1 (MQ=60)
                                        GGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAG                                                     <  SRR4302537.3318031/94‑1 (MQ=60)
                                         GTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGG                                                    >  SRR4302537.317464/1‑94 (MQ=60)
                                         GTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGG                                                    >  SRR4302537.3388828/1‑94 (MQ=60)
                                         GTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGG                                                    >  SRR4302537.5746865/1‑94 (MQ=60)
                                          TTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGC                                                   >  SRR4302537.6091527/1‑94 (MQ=60)
                                            CATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAACGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAT                                                 <  SRR4302537.4699415/94‑1 (MQ=60)
                                            CATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAT                                                 >  SRR4302537.896333/1‑94 (MQ=60)
                                            CATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAT                                                 <  SRR4302537.1882837/94‑1 (MQ=60)
                                            CATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAT                                                 >  SRR4302537.4939194/1‑94 (MQ=60)
                                            CATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAT                                                 >  SRR4302537.3918543/1‑94 (MQ=60)
                                            CATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCAT                                                 <  SRR4302537.2704569/94‑1 (MQ=60)
                                             ATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATT                                                >  SRR4302537.2735889/1‑94 (MQ=60)
                                              TATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTA                                               >  SRR4302537.6434055/1‑94 (MQ=60)
                                              TATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTA                                               <  SRR4302537.6014455/94‑1 (MQ=60)
                                               ATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTAC                                              <  SRR4302537.5869729/94‑1 (MQ=60)
                                                 TCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTT                                            <  SRR4302537.1761394/94‑1 (MQ=60)
                                                  CACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTT                                           >  SRR4302537.4371638/1‑94 (MQ=60)
                                                  CACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTA                                           >  SRR4302537.4063514/1‑94 (MQ=60)
                                                  CACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTA                                           <  SRR4302537.5680032/94‑1 (MQ=60)
                                                  CACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTA                                           >  SRR4302537.4371639/1‑94 (MQ=60)
                                                    CCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATC                                         <  SRR4302537.1964040/94‑1 (MQ=60)
                                                      TTTTACGATCAGTTGCGCCAGCTTCTAAGCCAGAACATACGAAAGGTTTTTCTGAGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTT                                       <  SRR4302537.2070274/94‑1 (MQ=60)
                                                        TTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCC                                     <  SRR4302537.5138677/94‑1 (MQ=60)
                                                         TAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCT                                    <  SRR4302537.317102/94‑1 (MQ=60)
                                                         TAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCT                                    >  SRR4302537.2903873/1‑94 (MQ=60)
                                                          AAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTT                                   <  SRR4302537.6151530/94‑1 (MQ=60)
                                                          AAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTT                                   <  SRR4302537.5303349/94‑1 (MQ=60)
                                                          AAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTT                                   <  SRR4302537.5532542/94‑1 (MQ=60)
                                                          AAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTT                                   <  SRR4302537.639360/94‑1 (MQ=60)
                                                          AAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTT                                   <  SRR4302537.3776001/94‑1 (MQ=60)
                                                           AGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTT                                  <  SRR4302537.3224812/94‑1 (MQ=60)
                                                           AGATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTT                                  >  SRR4302537.5808104/1‑94 (MQ=60)
                                                            GATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTT                                 >  SRR4302537.3732885/1‑94 (MQ=60)
                                                             ATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTT                                <  SRR4302537.4369344/94‑1 (MQ=60)
                                                             ATCCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTT                                >  SRR4302537.2352584/1‑94 (MQ=60)
                                                               CCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCT                              >  SRR4302537.5840203/1‑94 (MQ=60)
                                                               CCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCT                              <  SRR4302537.1749320/94‑1 (MQ=60)
                                                               CCGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCT                              <  SRR4302537.5101174/94‑1 (MQ=60)
                                                                CGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTT                             <  SRR4302537.5166625/94‑1 (MQ=60)
                                                                CGTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTT                             <  SRR4302537.3272658/94‑1 (MQ=60)
                                                                 GTTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTT                            >  SRR4302537.2113899/1‑94 (MQ=60)
                                                                  TTGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTT                           <  SRR4302537.1324135/94‑1 (MQ=60)
                                                                   TGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTT                          >  SRR4302537.3481243/1‑94 (MQ=60)
                                                                   TGCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTT                          <  SRR4302537.6522641/94‑1 (MQ=60)
                                                                    GCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTA                         <  SRR4302537.4673459/94‑1 (MQ=60)
                                                                    GCGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTA                         >  SRR4302537.4389758/1‑94 (MQ=60)
                                                                     CGCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTAT                        <  SRR4302537.4703911/94‑1 (MQ=60)
                                                                      GCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATT                       >  SRR4302537.1353360/1‑94 (MQ=60)
                                                                      GCCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATT                       >  SRR4302537.6501046/1‑94 (MQ=60)
                                                                      GCCAGCTTCTCAGCCAGAACATGCGAAAGGGTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATT                       >  SRR4302537.4102421/1‑94 (MQ=60)
                                                                       CCAGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTC                      <  SRR4302537.1926553/94‑1 (MQ=60)
                                                                         AGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCT                    <  SRR4302537.5264277/94‑1 (MQ=60)
                                                                         AGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCT                    <  SRR4302537.277387/94‑1 (MQ=60)
                                                                         AGCTTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCT                    >  SRR4302537.6441143/1‑94 (MQ=60)
                                                                            ctcaCAGCCAGAACATACGAAAAGTTATTCTGTGCTGCTAAGTGTGGTGCTCGTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCGCCT                 <  SRR4302537.3140092/90‑1 (MQ=60)
                                                                            TTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCT                 <  SRR4302537.406532/94‑1 (MQ=60)
                                                                            TTCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCT                 <  SRR4302537.4594264/94‑1 (MQ=60)
                                                                             TCTCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTT                <  SRR4302537.6435883/94‑1 (MQ=60)
                                                                               TCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAG              <  SRR4302537.4008488/94‑1 (MQ=60)
                                                                               TCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAG              <  SRR4302537.805219/94‑1 (MQ=60)
                                                                               TCAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAG              <  SRR4302537.3885050/94‑1 (MQ=60)
                                                                                CAGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGT             >  SRR4302537.3150042/1‑94 (MQ=60)
                                                                                 AGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTA            <  SRR4302537.5919159/94‑1 (MQ=60)
                                                                                 AGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTA            <  SRR4302537.161488/94‑1 (MQ=60)
                                                                                 AGCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTA            >  SRR4302537.14436/1‑94 (MQ=60)
                                                                                  GCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTAT           <  SRR4302537.4052186/94‑1 (MQ=60)
                                                                                  GCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTAT           <  SRR4302537.5910152/94‑1 (MQ=60)
                                                                                  GCCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTAT           <  SRR4302537.1858722/94‑1 (MQ=60)
                                                                                   CCAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATG          >  SRR4302537.2996783/1‑94 (MQ=60)
                                                                                    CAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGGTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGC         >  SRR4302537.3490139/1‑94 (MQ=60)
                                                                                    CAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGC         >  SRR4302537.60083/1‑94 (MQ=60)
                                                                                    CAGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGC         >  SRR4302537.2361113/1‑94 (MQ=60)
                                                                                     AGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCC        >  SRR4302537.5848490/1‑94 (MQ=60)
                                                                                     AGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCC        <  SRR4302537.3308581/94‑1 (MQ=60)
                                                                                     AGAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCC        >  SRR4302537.997721/1‑94 (MQ=60)
                                                                                      GAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCA       <  SRR4302537.1506371/94‑1 (MQ=60)
                                                                                      GAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCA       <  SRR4302537.5936750/94‑1 (MQ=60)
                                                                                      GAACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCA       <  SRR4302537.2188478/94‑1 (MQ=60)
                                                                                       AACATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCAC      >  SRR4302537.2272120/1‑94 (MQ=60)
                                                                                         CATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCA    <  SRR4302537.1164686/94‑1 (MQ=60)
                                                                                         CATGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTACTTAGTATGCCACCA    >  SRR4302537.1380334/1‑94 (MQ=60)
                                                                                           TGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGG  <  SRR4302537.4157178/94‑1 (MQ=60)
                                                                                           TGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGG  <  SRR4302537.4250199/94‑1 (MQ=60)
                                                                                           TGCGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGG  >  SRR4302537.2258201/1‑94 (MQ=60)
                                                                                            |                                                                                            
TTGCTCGCCCAGCTCAATCTCACCGGGCAAAATGGTGCCGGGTTCATATTCACCTTTTAAGATCCGTTGCGCCAGCTTCTCAGCCAGAACATACGAAAGGTTTTTCTGTGCAGCTAACTGTTGTGCGCTTAAAGGCATTACTTATCTTCCTTTTTCTTTTTATTCCTCCTTAGTATGCCACCAGG  >  NC_000913/3938813‑3938997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑