Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 647 270.1 21258240 98.4% 20918108 94.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,944,676 2 bp→GC intergenic (+20/+75) trpT → / ← yifDA tRNA‑Trp/transcription regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,944,6760CG99.8% 2232.3 / ‑5.4 638intergenic (+20/+76)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (310/327);  minor base A (1/0);  total (311/327)
Fisher's exact test for biased strand distribution p-value = 4.87e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.45e-01
*NC_0009133,944,6770GC100.0% 2264.9 / NA 638intergenic (+21/+75)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (311/327);  total (311/327)

Only 100 of 655 total aligned reads displayed.

GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA  >  NC_000913/3944583‑3944764
                                                                                             ||                                                                                       
      aGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAgg                                                                                    <  2:5257215/94‑1 (MQ=255)
      aGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAgg                                                                                    <  1:2601040/94‑1 (MQ=255)
       gTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                   >  1:7617/1‑94 (MQ=255)
       gTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                   >  1:709245/1‑94 (MQ=255)
       gTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                   >  2:8116296/1‑94 (MQ=255)
         tCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtt                                                                                 <  1:14572/94‑1 (MQ=255)
         tCAATTGGAAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtt                                                                                 >  1:3096852/1‑94 (MQ=255)
          cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAttt                                                                                >  1:10378510/1‑94 (MQ=255)
             ttGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttt                                                                             <  1:9216586/94‑1 (MQ=255)
                 tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAt                                                                         <  1:5151445/94‑1 (MQ=255)
                 tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAt                                                                         >  1:475009/1‑94 (MQ=255)
                 tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAt                                                                         >  1:4313654/1‑94 (MQ=255)
                 gagagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAt                                                                         <  2:9745765/93‑1 (MQ=255)
                  tgagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATc                                                                        >  1:2357721/2‑94 (MQ=255)
                  agagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATc                                                                        <  2:1081273/94‑1 (MQ=255)
                  agagCACCGGTCTCCAAAACCGGGTGGTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGTATTTTTTTTagc                                                                        >  2:3643437/1‑92 (MQ=255)
                    agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTg                                                                      <  2:5794349/94‑1 (MQ=255)
                      cACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGaa                                                                    >  2:9251108/1‑94 (MQ=255)
                        ccGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAt                                                                  <  1:3324583/94‑1 (MQ=255)
                        ccGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAt                                                                  <  1:4094935/94‑1 (MQ=255)
                        ccGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAt                                                                  <  1:5379406/94‑1 (MQ=255)
                         ctgTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATa                                                                 <  1:3844076/92‑1 (MQ=255)
                         cGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATa                                                                 >  1:8157481/1‑94 (MQ=255)
                         cGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATa                                                                 <  2:9412382/94‑1 (MQ=255)
                           gTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAc                                                               <  2:5890311/94‑1 (MQ=255)
                           gTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAc                                                               <  1:9369588/94‑1 (MQ=255)
                            tctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATaagc                                                              >  1:3567722/1‑92 (MQ=255)
                            tctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAcc                                                              >  1:957644/1‑94 (MQ=255)
                            tctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAcc                                                              <  2:9049232/94‑1 (MQ=255)
                             ctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAccc                                                             >  2:7217341/1‑94 (MQ=255)
                             ctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAccc                                                             >  2:991011/1‑94 (MQ=255)
                              tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                            <  2:1303220/94‑1 (MQ=255)
                               ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                           <  1:4572957/94‑1 (MQ=255)
                               ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                           <  2:5105465/94‑1 (MQ=255)
                                cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctct                                                          <  1:699333/94‑1 (MQ=255)
                                 aaaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCtctc                                                         <  2:8797578/94‑1 (MQ=255)
                                  aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTcc                                                        >  1:4469640/1‑94 (MQ=255)
                                  aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTcc                                                        >  2:9610601/1‑94 (MQ=255)
                                    aCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGa                                                      <  1:10232287/94‑1 (MQ=255)
                                    aCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGa                                                      >  2:4518374/1‑94 (MQ=255)
                                       gggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGt                                                   <  1:2735628/94‑1 (MQ=255)
                                        ggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTa                                                  >  2:7523880/1‑94 (MQ=255)
                                           gtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAt                                               >  2:355261/1‑94 (MQ=255)
                                            ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATc                                              >  2:6465797/1‑94 (MQ=255)
                                            ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATc                                              >  2:1948615/1‑94 (MQ=255)
                                               ggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCtt                                           <  1:1341391/94‑1 (MQ=255)
                                               ggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCtt                                           <  1:2896340/94‑1 (MQ=255)
                                               ggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCtt                                           <  2:6157081/94‑1 (MQ=255)
                                               ggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCtt                                           <  2:1484484/94‑1 (MQ=255)
                                                  gTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTa                                        <  2:2635449/94‑1 (MQ=255)
                                                  gTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTa                                        >  2:6974980/1‑94 (MQ=255)
                                                  gTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTa                                        <  1:1945187/94‑1 (MQ=255)
                                                     cGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCg                                     <  1:9868486/94‑1 (MQ=255)
                                                     cGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCg                                     >  1:1013250/1‑94 (MQ=255)
                                                       aGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGc                                   >  1:2150481/1‑94 (MQ=255)
                                                        gTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCa                                  >  2:4281184/1‑94 (MQ=255)
                                                        gTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCa                                  >  1:3989955/1‑94 (MQ=255)
                                                         tctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCAt                                 <  2:7427862/94‑1 (MQ=255)
                                                            ctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCt                              >  2:8680073/1‑94 (MQ=255)
                                                             tcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCtt                             >  2:8942974/1‑94 (MQ=255)
                                                             tcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCtt                             <  1:7807714/94‑1 (MQ=255)
                                                             tcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCtt                             <  2:2317763/94‑1 (MQ=255)
                                                             cccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCtt                             <  2:3941009/93‑1 (MQ=255)
                                                              ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTg                            <  2:3438019/94‑1 (MQ=255)
                                                              ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTg                            <  2:2178186/94‑1 (MQ=255)
                                                              ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTg                            <  1:10533979/94‑1 (MQ=255)
                                                              ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTg                            <  2:4770402/94‑1 (MQ=255)
                                                               cGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgc                           >  1:597052/1‑94 (MQ=255)
                                                                gCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcc                          >  2:7758588/1‑94 (MQ=255)
                                                                 ccgcTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                         >  1:7319998/4‑94 (MQ=255)
                                                                 ccccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                         <  1:10007181/94‑1 (MQ=255)
                                                                  cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccag                        <  1:1748918/94‑1 (MQ=255)
                                                                  cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccag                        <  1:8332610/94‑1 (MQ=255)
                                                                   ccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGGAGTAAATCCTTCTACCGGCCTCCTTgccagc                       >  2:10397107/1‑94 (MQ=255)
                                                                   ccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                       <  1:1150978/94‑1 (MQ=255)
                                                                     tGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcca                     >  1:6713798/1‑94 (MQ=255)
                                                                      gCCAGAAATCATCCTTAGCGAAAGCTAATGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAt                    <  1:7929329/94‑1 (MQ=255)
                                                                       ccAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAtt                   >  2:10217496/1‑94 (MQ=255)
                                                                        cAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTc                  <  1:8506169/94‑1 (MQ=255)
                                                                        cAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTc                  <  2:4924844/94‑1 (MQ=255)
                                                                         aGAACTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCa                 <  1:9670133/94‑1 (MQ=255)
                                                                          gAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCat                >  2:290301/1‑94 (MQ=255)
                                                                          gAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCat                >  1:4958455/1‑94 (MQ=255)
                                                                          gAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCat                >  2:10064809/1‑94 (MQ=255)
                                                                            aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat              >  1:8950635/1‑94 (MQ=255)
                                                                             atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAtt             >  2:560763/1‑94 (MQ=255)
                                                                             atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAtt             >  2:7907848/1‑94 (MQ=255)
                                                                             atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTGGCCAGCAATTCATAtt             >  1:4818036/1‑94 (MQ=255)
                                                                              tcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTa            >  2:4164524/1‑94 (MQ=255)
                                                                              tcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTa            >  2:3069372/1‑94 (MQ=255)
                                                                               catcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa           >  1:6458292/1‑94 (MQ=255)
                                                                                  ccTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac        <  2:666342/94‑1 (MQ=255)
                                                                                  ccTTAGCGAAAGCTAAAGATTTTTTGTATCTAAAATATCCCCCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac        <  2:5423412/94‑1 (MQ=255)
                                                                                    ttAGCGAAGCCTAAGTATTTTTTCTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATacac      <  2:2746065/94‑1 (MQ=255)
                                                                                    ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATacac      >  2:8336967/1‑94 (MQ=255)
                                                                                    ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATacac      >  1:5866346/1‑94 (MQ=255)
                                                                                      aGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACtt    <  1:3724693/94‑1 (MQ=255)
                                                                                      aGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACtt    >  1:5665863/1‑94 (MQ=255)
                                                                                       gCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTc   <  1:6220738/94‑1 (MQ=255)
                                                                                       gCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGGAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTc   >  2:8472774/1‑94 (MQ=255)
                                                                                             ||                                                                                       
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA  >  NC_000913/3944583‑3944764

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 383 total aligned reads displayed.
GTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCAAGCTGG  >  NC_000913/3944986‑3945172
                                                                                             |                                                                                             
 TCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGC                                                                                              <  SRR4302545.8064673/94‑1 (MQ=60)
  CAGCACCGTTCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCT                                                                                             <  SRR4302545.1113737/94‑1 (MQ=60)
   AGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTG                                                                                            <  SRR4302545.3259220/94‑1 (MQ=60)
   AGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTG                                                                                            >  SRR4302545.9016283/1‑94 (MQ=60)
    GCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGG                                                                                           <  SRR4302545.3005001/94‑1 (MQ=60)
    GCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGG                                                                                           <  SRR4302545.2594857/94‑1 (MQ=60)
     CACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGT                                                                                          <  SRR4302545.5208993/94‑1 (MQ=60)
     CACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGT                                                                                          >  SRR4302545.5784136/1‑94 (MQ=60)
     CACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGT                                                                                          >  SRR4302545.8773434/1‑94 (MQ=60)
      ACCGATCAAACCTGCCTCATGCTGTTGAAAGTCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTA                                                                                         >  SRR4302545.6708003/1‑94 (MQ=60)
      ACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTA                                                                                         >  SRR4302545.1530187/1‑94 (MQ=60)
      ACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTA                                                                                         <  SRR4302545.2742445/94‑1 (MQ=60)
      ACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTA                                                                                         >  SRR4302545.940213/1‑94 (MQ=60)
      ACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTA                                                                                         <  SRR4302545.2737676/94‑1 (MQ=60)
       CCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTAT                                                                                        <  SRR4302545.1002759/94‑1 (MQ=60)
       CCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTAT                                                                                        <  SRR4302545.6844850/94‑1 (MQ=60)
        CGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATA                                                                                       >  SRR4302545.4024774/1‑94 (MQ=60)
        CGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATA                                                                                       >  SRR4302545.6438833/1‑94 (MQ=60)
        CGATCAAACCTGCCCCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATA                                                                                       >  SRR4302545.7392200/1‑94 (MQ=60)
        CGATCAAACCTGCATCAGGCTCTTGAAAGTCTGGACCCCACTCAAGTCGCAGAGAATTAAGATCTCCCTTTAGACTTGAAGCGGCGCTGTTATA                                                                                       >  SRR4302545.7223618/1‑94 (MQ=40)
         GATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAA                                                                                      >  SRR4302545.1457763/1‑94 (MQ=60)
         GATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAA                                                                                      >  SRR4302545.10506578/1‑94 (MQ=60)
         GATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAA                                                                                      <  SRR4302545.6466265/94‑1 (MQ=60)
          ATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAA                                                                                     <  SRR4302545.6834573/94‑1 (MQ=60)
          ATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAA                                                                                     >  SRR4302545.9842783/1‑94 (MQ=60)
           TCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAG                                                                                    <  SRR4302545.3715083/94‑1 (MQ=60)
           TCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAG                                                                                    >  SRR4302545.10003667/1‑94 (MQ=60)
           TCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAG                                                                                    <  SRR4302545.2886187/94‑1 (MQ=60)
            CAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGC                                                                                   <  SRR4302545.1483871/94‑1 (MQ=60)
            CAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGC                                                                                   >  SRR4302545.10620381/1‑94 (MQ=60)
            CAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGC                                                                                   <  SRR4302545.9053325/94‑1 (MQ=60)
            CAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGC                                                                                   >  SRR4302545.10003693/1‑94 (MQ=60)
             AAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCG                                                                                  >  SRR4302545.2194626/1‑94 (MQ=60)
             AAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCG                                                                                  >  SRR4302545.5838961/1‑94 (MQ=60)
             AAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCG                                                                                  >  SRR4302545.5272504/1‑94 (MQ=60)
              AACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGC                                                                                 >  SRR4302545.9358491/1‑94 (MQ=60)
              AACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGC                                                                                 >  SRR4302545.6130324/1‑94 (MQ=60)
               ACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGTGCGCTGGTATAAAGCGCT                                                                                <  SRR4302545.9095108/94‑1 (MQ=60)
               ACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCT                                                                                >  SRR4302545.5940276/1‑94 (MQ=60)
               ACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCT                                                                                <  SRR4302545.2404667/94‑1 (MQ=60)
               ACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCT                                                                                <  SRR4302545.7957764/94‑1 (MQ=60)
                CCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTA                                                                               >  SRR4302545.1079216/1‑94 (MQ=60)
                CCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTA                                                                               <  SRR4302545.10481672/94‑1 (MQ=60)
                CCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTA                                                                               <  SRR4302545.3187911/94‑1 (MQ=60)
                CCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTA                                                                               <  SRR4302545.4521429/94‑1 (MQ=60)
                 CTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAA                                                                              <  SRR4302545.2536215/94‑1 (MQ=60)
                 CTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAA                                                                              >  SRR4302545.559313/1‑94 (MQ=60)
                 CTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAA                                                                              >  SRR4302545.9509398/1‑94 (MQ=60)
                  TGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAA                                                                             <  SRR4302545.4806142/94‑1 (MQ=60)
                    CCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGT                                                                           >  SRR4302545.6232106/1‑94 (MQ=60)
                    CCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGT                                                                           >  SRR4302545.9811212/1‑94 (MQ=60)
                     CTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTG                                                                          <  SRR4302545.4839712/94‑1 (MQ=60)
                     CTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTG                                                                          <  SRR4302545.8372755/94‑1 (MQ=60)
                      TCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGA                                                                         >  SRR4302545.7476382/1‑94 (MQ=60)
                       CATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAA                                                                        >  SRR4302545.3849142/1‑94 (MQ=60)
                       CATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAA                                                                        >  SRR4302545.9531037/1‑94 (MQ=60)
                       CATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAA                                                                        >  SRR4302545.4111391/1‑94 (MQ=60)
                       CATGCTGTTGAAAATCTGGACCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAA                                                                        >  SRR4302545.4661351/1‑94 (MQ=60)
                        ATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAA                                                                       >  SRR4302545.10224516/1‑94 (MQ=60)
                         TGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAAT                                                                      <  SRR4302545.8393425/94‑1 (MQ=60)
                         TGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAAT                                                                      <  SRR4302545.5810572/94‑1 (MQ=60)
                         TGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAAT                                                                      <  SRR4302545.3339643/94‑1 (MQ=60)
                           CTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATAT                                                                    >  SRR4302545.3929143/1‑94 (MQ=60)
                           CTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATAT                                                                    >  SRR4302545.9320383/1‑94 (MQ=60)
                           CTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATAT                                                                    >  SRR4302545.1227753/1‑94 (MQ=60)
                           CTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATAT                                                                    <  SRR4302545.9837226/94‑1 (MQ=60)
                            TGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATC                                                                   >  SRR4302545.11430/1‑94 (MQ=60)
                            TGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATC                                                                   <  SRR4302545.1829725/94‑1 (MQ=60)
                            TGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATC                                                                   >  SRR4302545.10169633/1‑94 (MQ=60)
                            TGTGGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATC                                                                   <  SRR4302545.5462190/94‑1 (MQ=60)
                             GTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCC                                                                  >  SRR4302545.5378292/1‑94 (MQ=60)
                             GTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCC                                                                  <  SRR4302545.6559522/94‑1 (MQ=60)
                             GTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCC                                                                  >  SRR4302545.8630661/1‑94 (MQ=60)
                             GTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCC                                                                  <  SRR4302545.7826097/94‑1 (MQ=60)
                              TTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCC                                                                 <  SRR4302545.7059140/94‑1 (MQ=60)
                              TTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCC                                                                 >  SRR4302545.1090685/1‑94 (MQ=60)
                              TTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCC                                                                 <  SRR4302545.5151721/94‑1 (MQ=60)
                              TTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCC                                                                 <  SRR4302545.4433668/94‑1 (MQ=60)
                               TGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCA                                                                <  SRR4302545.345526/94‑1 (MQ=60)
                               TGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCA                                                                >  SRR4302545.4745497/1‑94 (MQ=60)
                               TGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCA                                                                <  SRR4302545.4145974/94‑1 (MQ=60)
                               TGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCA                                                                >  SRR4302545.6118486/1‑94 (MQ=60)
                                GAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAG                                                               >  SRR4302545.9172420/1‑94 (MQ=60)
                                 AAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCTCAGC                                                              >  SRR4302545.9876789/1‑94 (MQ=60)
                                   AATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAA                                                            >  SRR4302545.7764222/1‑94 (MQ=60)
                                    ATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAAC                                                           >  SRR4302545.9424724/1‑94 (MQ=60)
                                     TCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACT                                                          <  SRR4302545.2188777/94‑1 (MQ=60)
                                     TCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACT                                                          <  SRR4302545.6723160/94‑1 (MQ=60)
                                     TCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACT                                                          <  SRR4302545.9723951/94‑1 (MQ=60)
                                     TCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACT                                                          <  SRR4302545.5234274/94‑1 (MQ=60)
                                     TCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACT                                                          <  SRR4302545.3359090/94‑1 (MQ=60)
                                      CTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTG                                                         <  SRR4302545.8427344/94‑1 (MQ=60)
                                       TGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGA                                                        <  SRR4302545.872376/94‑1 (MQ=60)
                                        GGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGAC                                                       <  SRR4302545.9033818/94‑1 (MQ=60)
                                        GGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGAC                                                       <  SRR4302545.5632420/94‑1 (MQ=60)
                                         GCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACT                                                      >  SRR4302545.4450654/1‑94 (MQ=60)
                                         GCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACT                                                      <  SRR4302545.772511/94‑1 (MQ=60)
                                         GCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACT                                                      <  SRR4302545.1073854/94‑1 (MQ=60)
                                         GCCCCCACTCAAGTCGCAGATAACTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACT                                                      <  SRR4302545.6027727/94‑1 (MQ=60)
                                          CCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTA                                                     >  SRR4302545.1820146/1‑94 (MQ=60)
                                          CCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTA                                                     >  SRR4302545.4315742/1‑94 (MQ=60)
                                            CCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACT                                                   <  SRR4302545.1270910/94‑1 (MQ=60)
                                            CCCACTCAAGTCGCAGATAATTAAGATCTCCATTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTACAGTGAATTATCCCAGCAACTGACTACT                                                   >  SRR4302545.1184178/1‑94 (MQ=60)
                                             CCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTA                                                  >  SRR4302545.5900903/1‑94 (MQ=60)
                                             CCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTA                                                  >  SRR4302545.609886/1‑94 (MQ=60)
                                             CCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTA                                                  >  SRR4302545.4243619/1‑94 (MQ=60)
                                              CACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAA                                                 <  SRR4302545.2316911/94‑1 (MQ=60)
                                               ACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAA                                                <  SRR4302545.5958774/94‑1 (MQ=60)
                                               ACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAA                                                <  SRR4302545.5512387/94‑1 (MQ=60)
                                               ACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAA                                                <  SRR4302545.2050533/94‑1 (MQ=60)
                                                CTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAATGTGAAATATCCCAGCAACTGACTACTAAAT                                               >  SRR4302545.9970841/1‑94 (MQ=60)
                                                CTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAAT                                               <  SRR4302545.10067061/94‑1 (MQ=60)
                                                CTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAAT                                               >  SRR4302545.2764787/1‑94 (MQ=60)
                                                 TCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATT                                              >  SRR4302545.9948161/1‑94 (MQ=60)
                                                  CAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTC                                             >  SRR4302545.2711391/1‑94 (MQ=60)
                                                  CAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTC                                             >  SRR4302545.4178082/1‑94 (MQ=60)
                                                  CAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTC                                             >  SRR4302545.5766145/1‑94 (MQ=60)
                                                  CAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTC                                             <  SRR4302545.10220308/94‑1 (MQ=60)
                                                   AAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCG                                            <  SRR4302545.6831350/94‑1 (MQ=60)
                                                    AGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGC                                           >  SRR4302545.2973330/1‑94 (MQ=60)
                                                       CGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCA                                        >  SRR4302545.4965840/1‑94 (MQ=60)
                                                       CGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCA                                        <  SRR4302545.7096368/94‑1 (MQ=60)
                                                       CGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCA                                        >  SRR4302545.6978535/1‑94 (MQ=60)
                                                       CGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCA                                        >  SRR4302545.4567172/1‑94 (MQ=60)
                                                       CGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGCATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCA                                        >  SRR4302545.8694665/1‑94 (MQ=60)
                                                        GCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAAGATCCCAGCAACTGACTACGAAATTCGTCCAT                                       <  SRR4302545.7808501/94‑1 (MQ=60)
                                                         CAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATT                                      >  SRR4302545.4031444/1‑94 (MQ=60)
                                                         CAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATT                                      <  SRR4302545.10881472/94‑1 (MQ=60)
                                                         CAGATAATTAAGATCTACCATTAGTTTTGAAGGGGTGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATT                                      >  SRR4302545.7559882/1‑94 (MQ=60)
                                                             TAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCGTTTTGG                                  >  SRR4302545.7647467/1‑94 (MQ=60)
                                                             TAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGG                                  <  SRR4302545.8256332/94‑1 (MQ=60)
                                                             TAATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGG                                  <  SRR4302545.10373310/94‑1 (MQ=60)
                                                              AATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGG                                 >  SRR4302545.2835681/1‑94 (MQ=60)
                                                              AATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGG                                 >  SRR4302545.4900860/1‑94 (MQ=60)
                                                              AATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCCGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGG                                 <  SRR4302545.4977532/94‑1 (MQ=60)
                                                               ATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGC                                <  SRR4302545.4916106/94‑1 (MQ=60)
                                                               ATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGC                                <  SRR4302545.8454761/94‑1 (MQ=60)
                                                               ATTAAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGC                                >  SRR4302545.942986/1‑94 (MQ=60)
                                                                  AAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCT                             >  SRR4302545.5537097/1‑94 (MQ=60)
                                                                  AAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCT                             <  SRR4302545.6754596/94‑1 (MQ=60)
                                                                  AAGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCT                             <  SRR4302545.8791946/94‑1 (MQ=60)
                                                                   AGATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTT                            <  SRR4302545.3904925/94‑1 (MQ=60)
                                                                    GATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTC                           <  SRR4302545.1072125/94‑1 (MQ=60)
                                                                    GATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTC                           >  SRR4302545.8851749/1‑94 (MQ=60)
                                                                     acccCCCTTTAGTTTTAAGGGGGCGTTGGTATAAAGCGTTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCA                          <  SRR4302545.8813773/90‑1 (MQ=60)
                                                                     ATCTCCCTTTTGTTTTGAATGGGCACGGGGATAAAGCGCCAAAGGGAAACATCTCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTCCA                          >  SRR4302545.8320998/1‑94 (MQ=57)
                                                                     ATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCA                          <  SRR4302545.775604/94‑1 (MQ=60)
                                                                     ATCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCA                          <  SRR4302545.3399393/94‑1 (MQ=60)
                                                                      TCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAG                         <  SRR4302545.5075089/94‑1 (MQ=60)
                                                                      TCTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTCGGCGCTTCAG                         >  SRR4302545.4749222/1‑94 (MQ=60)
                                                                       CTCCCTTTAGTTTTTAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGT                        <  SRR4302545.3235789/94‑1 (MQ=60)
                                                                       CTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGT                        >  SRR4302545.5156715/1‑94 (MQ=60)
                                                                       CTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGT                        >  SRR4302545.4414508/1‑94 (MQ=60)
                                                                       CTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGT                        <  SRR4302545.6362852/94‑1 (MQ=60)
                                                                       CTCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGT                        <  SRR4302545.6763334/94‑1 (MQ=60)
                                                                        TCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTG                       <  SRR4302545.10548318/94‑1 (MQ=60)
                                                                        TCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTG                       <  SRR4302545.463318/94‑1 (MQ=60)
                                                                        TCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTG                       <  SRR4302545.3946047/94‑1 (MQ=60)
                                                                        TCCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTG                       <  SRR4302545.7819580/94‑1 (MQ=60)
                                                                         CCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGG                      <  SRR4302545.7753247/94‑1 (MQ=60)
                                                                         CCCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGG                      <  SRR4302545.10521837/94‑1 (MQ=60)
                                                                          CCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGT                     >  SRR4302545.5759746/1‑94 (MQ=60)
                                                                          CCTTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGA                     >  SRR4302545.3830346/1‑94 (MQ=60)
                                                                            CTTAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAA                   <  SRR4302545.1599290/94‑1 (MQ=60)
                                                                              TAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATA                 <  SRR4302545.7901080/94‑1 (MQ=60)
                                                                              TAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATA                 >  SRR4302545.7316302/1‑94 (MQ=60)
                                                                              TAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATA                 >  SRR4302545.5928205/1‑94 (MQ=60)
                                                                              TAGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATA                 >  SRR4302545.3764166/1‑94 (MQ=60)
                                                                               AGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAA                >  SRR4302545.3721372/1‑94 (MQ=60)
                                                                               AGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAA                <  SRR4302545.1337307/94‑1 (MQ=60)
                                                                               AGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAA                <  SRR4302545.10111297/94‑1 (MQ=60)
                                                                               AGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAA                <  SRR4302545.9175188/94‑1 (MQ=60)
                                                                               AGTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAA                >  SRR4302545.5644250/1‑94 (MQ=60)
                                                                                GTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAG               <  SRR4302545.4255900/94‑1 (MQ=60)
                                                                                GTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAG               >  SRR4302545.1858440/1‑94 (MQ=60)
                                                                                GTTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAA               >  SRR4302545.2431245/1‑94 (MQ=60)
                                                                                 TTTTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGA              >  SRR4302545.4898583/1‑94 (MQ=60)
                                                                                   TTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATCAGAAG            >  SRR4302545.7134701/1‑94 (MQ=60)
                                                                                   TTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAG            <  SRR4302545.10607973/94‑1 (MQ=60)
                                                                                   TTGAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAG            <  SRR4302545.3128512/94‑1 (MQ=60)
                                                                                     GAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGAT          >  SRR4302545.7543527/1‑94 (MQ=60)
                                                                                     GAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGAT          >  SRR4302545.2722836/1‑94 (MQ=60)
                                                                                     GAAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGAT          <  SRR4302545.1400726/94‑1 (MQ=60)
                                                                                     GAAGGGGCGCGGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGAT          <  SRR4302545.6960090/94‑1 (MQ=60)
                                                                                      AAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATC         >  SRR4302545.3416116/1‑94 (MQ=60)
                                                                                      AAGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATC         >  SRR4302545.3556366/1‑94 (MQ=60)
                                                                                       TGGGGCGCTGGTATAAAGCGCTAAATTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTTGGCGCTTCAGTGGTAATAAGAAGATCA        >  SRR4302545.2649657/1‑94 (MQ=60)
                                                                                       AGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCTCTTCAGTGGTAATAAGAAGATCA        >  SRR4302545.6022389/1‑94 (MQ=60)
                                                                                       AGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCA        >  SRR4302545.4699012/1‑94 (MQ=60)
                                                                                       AGGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCA        <  SRR4302545.8745879/94‑1 (MQ=60)
                                                                                        GGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCAA       <  SRR4302545.5025357/94‑1 (MQ=60)
                                                                                        GGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCAA       >  SRR4302545.3028332/1‑94 (MQ=60)
                                                                                        GGGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCAA       >  SRR4302545.829721/1‑94 (MQ=60)
                                                                                         GGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCAAG      <  SRR4302545.2134365/94‑1 (MQ=60)
                                                                                         GGGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTCGTAATAAGAAGATCAAG      <  SRR4302545.10330032/94‑1 (MQ=60)
                                                                                          GGCGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCAAGC     <  SRR4302545.10805016/94‑1 (MQ=60)
                                                                                            CGCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCAAGCTG   <  SRR4302545.2956121/94‑1 (MQ=60)
                                                                                             GCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCAAGCTGG  <  SRR4302545.3115904/94‑1 (MQ=60)
                                                                                             GCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCAAGCTGG  <  SRR4302545.10191011/94‑1 (MQ=60)
                                                                                             GCTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCAAGCTGG  <  SRR4302545.3101736/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTCAGCACCGATCAAACCTGCCTCATGCTGTTGAAAATCTGGCCCCCACTCAAGTCGCAGATAATTAAGATCTCCCTTTAGTTTTGAAGGGGCACTGGTATAAAGCGCTAAAGTGAAATATCCCAGCAACTGACTACTAAATTCGTCCATTTTGGGCGCTTCAGTGGTAATAAGAAGATCAAGCTGG  >  NC_000913/3944986‑3945172

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑