Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,255,594 Δ1 bp coding (4/1278 nt) yhaO ← putative transport system permease protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,255,5930G.100.0% 1182.0 / NA 252coding (5/1278 nt)yhaOputative transport system permease protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (113/139);  total (113/139)

Only 100 of 273 total aligned reads displayed.

CGCCAATCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTT  >  NC_000913/3255501‑3255682
                                                                                            |                                                                                         
  ccAATCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAgca                                                                                         >  1:1137361/1‑91 (MQ=255)
   cAATCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAgcac                                                                                        >  1:2200/1‑90 (MQ=255)
    aaTCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAgcacg                                                                                       >  2:4301936/1‑89 (MQ=255)
     aTCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGa                                                                                     >  2:1413073/1‑93 (MQ=255)
      tCGCCATCCCGATCCTCATAATCACCCAGCCAGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGAc                                                                                    <  1:3791067/93‑1 (MQ=255)
      tCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGAc                                                                                    <  1:3728147/93‑1 (MQ=255)
        gCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGAccc                                                                                  >  2:3554679/1‑93 (MQ=255)
         ccATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACccg                                                                                 <  1:3506963/93‑1 (MQ=255)
          cATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACccgc                                                                                >  2:3850021/1‑93 (MQ=255)
           aTCCTGATATTCATAATCTCCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACccgcc                                                                               <  2:496126/93‑1 (MQ=255)
            tCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACccgccg                                                                              >  2:6197150/1‑93 (MQ=255)
            tCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACccgccg                                                                              >  1:5991793/1‑93 (MQ=255)
             cccGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCgg                                                                             <  2:6402602/93‑1 (MQ=255)
             cccGATACTCATAACCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCgg                                                                             <  1:5637368/93‑1 (MQ=255)
              ccGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCggg                                                                            >  2:2046922/1‑93 (MQ=255)
              ccGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCggg                                                                            >  2:2096595/1‑93 (MQ=255)
              ccGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCggg                                                                            >  1:543312/1‑93 (MQ=255)
              ccGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCggg                                                                            >  2:5760756/1‑93 (MQ=255)
                gATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGt                                                                          >  1:4250142/1‑93 (MQ=255)
                gATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGt                                                                          >  1:1462178/1‑93 (MQ=255)
                   aCTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGa                                                                       <  1:3550458/93‑1 (MQ=255)
                   aCTCATAATCACCAAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGa                                                                       >  2:3681000/1‑93 (MQ=255)
                    cTCATAATCGCCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGaa                                                                      <  2:2576110/93‑1 (MQ=255)
                    cTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGaa                                                                      >  2:528921/1‑93 (MQ=255)
                     tCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAg                                                                     <  1:1163282/93‑1 (MQ=255)
                      cATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAgc                                                                    >  1:1849248/1‑92 (MQ=255)
                       aTAATCCCCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAgcg                                                                   <  1:2446169/93‑3 (MQ=255)
                       aTAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAgcg                                                                   >  2:5512426/1‑91 (MQ=255)
                        tAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAgcgt                                                                  >  1:6394560/1‑90 (MQ=255)
                        tAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAgcgt                                                                  <  1:4890939/93‑4 (MQ=255)
                         aaTCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAgcgtc                                                                 <  2:1555222/93‑5 (MQ=255)
                          aTCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCt                                                               <  1:1244560/93‑1 (MQ=255)
                           tCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTg                                                              >  2:6117649/1‑93 (MQ=255)
                               ccAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAt                                                          <  2:1774854/93‑1 (MQ=255)
                                 atccGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGa                                                        <  2:3947241/91‑1 (MQ=255)
                                 aGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAGTGa                                                        >  1:1679327/1‑93 (MQ=255)
                                 aGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGa                                                        <  1:2859308/93‑1 (MQ=255)
                                 aGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGCCGAA‑GCGTCTGCAATGa                                                        >  2:6056877/1‑93 (MQ=255)
                                    cGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGc                                                     <  2:2733983/93‑1 (MQ=255)
                                    cGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGc                                                     <  2:2271236/93‑1 (MQ=255)
                                     ggTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCTCTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACTcc                                                    <  2:3039887/93‑1 (MQ=255)
                                      gtgtCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCt                                                   >  1:3342370/1‑93 (MQ=255)
                                        gtCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCttt                                                 <  2:4814534/93‑1 (MQ=255)
                                        gtCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCttt                                                 <  1:2051779/93‑1 (MQ=255)
                                         tCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTGATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTa                                                >  2:2666994/1‑93 (MQ=255)
                                         tCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTa                                                >  1:2417380/1‑93 (MQ=255)
                                          cAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAt                                               <  2:360894/93‑1 (MQ=255)
                                            gTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTc                                             <  1:923418/93‑1 (MQ=255)
                                            gTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTc                                             >  2:773854/1‑93 (MQ=255)
                                             tACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCg                                            <  1:4477207/93‑1 (MQ=255)
                                              aCTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGa                                           <  2:2497449/93‑1 (MQ=255)
                                               cTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGAt                                          >  1:3205786/1‑93 (MQ=255)
                                                  tCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGTCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCa                                       >  1:1950609/1‑93 (MQ=255)
                                                    gAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAt                                     <  2:3057488/93‑1 (MQ=255)
                                                    gAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAt                                     <  1:5313065/93‑1 (MQ=255)
                                                     aaTTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAtt                                    <  2:6414129/93‑1 (MQ=255)
                                                       tttGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTc                                  <  1:5707552/93‑1 (MQ=255)
                                                       tttGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTc                                  <  1:5574527/93‑1 (MQ=255)
                                                       tttGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTc                                  <  1:5180628/93‑1 (MQ=255)
                                                        ttGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTcc                                 >  1:1256482/1‑93 (MQ=255)
                                                         tGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCa                                <  1:622096/93‑1 (MQ=255)
                                                         tGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCa                                >  1:4627901/1‑93 (MQ=255)
                                                          gATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCAt                               >  2:6246127/1‑93 (MQ=255)
                                                          gATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCAt                               <  1:6059969/93‑1 (MQ=255)
                                                          gATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCAt                               >  2:5857448/1‑93 (MQ=255)
                                                          gATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCAt                               >  2:1960514/1‑93 (MQ=255)
                                                            tCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATaa                             <  2:909239/93‑1 (MQ=255)
                                                            tCGCTCCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATaa                             <  2:160049/93‑1 (MQ=255)
                                                             cGCTTCTCGCCTCTCGCTCTGACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAAt                            <  2:5438976/93‑1 (MQ=255)
                                                                ttCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTtc                         >  1:5821891/1‑93 (MQ=255)
                                                                ttCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTtc                         >  2:1111793/1‑93 (MQ=255)
                                                                    cGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGc                     >  1:2520672/1‑93 (MQ=255)
                                                                    cGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGc                     <  2:446523/93‑1 (MQ=255)
                                                                    cGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGc                     <  2:1039747/93‑1 (MQ=255)
                                                                       cACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCa                  <  2:1803730/93‑1 (MQ=255)
                                                                        aCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCaa                 <  1:549/93‑1 (MQ=255)
                                                                         cTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCTTCTGCAATGACGCCTTTATTCGATTCAATTTCCATAATTTCTCGCTCAat                <  2:2906400/93‑1 (MQ=255)
                                                                          tCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAata               <  1:4062171/93‑1 (MQ=255)
                                                                           cGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAatat              <  2:4386566/93‑1 (MQ=255)
                                                                            gCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAtt             >  2:1892926/1‑93 (MQ=255)
                                                                             ctctCACTCATTCCA‑GAACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAttt            >  2:4194965/1‑93 (MQ=255)
                                                                             ctctCACCCATACCA‑GCCCGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAttt            <  2:3451809/93‑1 (MQ=255)
                                                                              tctcACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAtttt           <  2:1286614/93‑1 (MQ=255)
                                                                              tctcACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAtttt           >  2:3312501/1‑93 (MQ=255)
                                                                               ctcactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTg          <  2:3187104/93‑1 (MQ=255)
                                                                                tcactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGt         <  1:1773621/93‑1 (MQ=255)
                                                                                 cactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGCCGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTa        <  1:4121513/93‑1 (MQ=255)
                                                                                 cactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTa        <  1:2613403/93‑1 (MQ=255)
                                                                                 cactcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTa        >  1:1010092/1‑93 (MQ=255)
                                                                                  actcaTTCCA‑GCACGCCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAg       <  2:1359967/93‑1 (MQ=255)
                                                                                   ctcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAgg      <  1:3879029/93‑1 (MQ=255)
                                                                                   ctcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAgg      >  1:2295694/1‑93 (MQ=255)
                                                                                   ctcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑ACGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTacg      >  2:5626766/1‑91 (MQ=255)
                                                                                    tcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAggg     <  1:50518/93‑1 (MQ=255)
                                                                                    tcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAggg     >  2:4387986/1‑93 (MQ=255)
                                                                                    tcaTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAggg     <  1:3400319/93‑1 (MQ=255)
                                                                                     caTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGc    <  1:2907646/93‑1 (MQ=255)
                                                                                      aTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCt   >  2:374038/1‑93 (MQ=255)
                                                                                      aTTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCt   >  1:1557909/1‑93 (MQ=255)
                                                                                       ttCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCtt  <  2:3489272/93‑1 (MQ=255)
                                                                                            |                                                                                         
CGCCAATCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTT  >  NC_000913/3255501‑3255682

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCAATCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAT  >  NC_000913/3255503‑3255687
                                                                                           |                                                                                             
CCAATCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCAC                                                                                            >  SRR4302537.2215/1‑94 (MQ=60)
CCAATCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCAC                                                                                            <  SRR4302537.1764452/94‑1 (MQ=60)
CCAATCCCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCAC                                                                                            <  SRR4302537.1240548/94‑1 (MQ=60)
  AATCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGA                                                                                          >  SRR4302537.981683/1‑94 (MQ=60)
   ATCGCCATCCCGATACTCATAATCCCCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGAC                                                                                         <  SRR4302537.85999/94‑1 (MQ=60)
   ATCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGAC                                                                                         >  SRR4302537.2487275/1‑94 (MQ=60)
    TCGCCATCCCGATCCTCATAATCACCCAGCCAGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACC                                                                                        <  SRR4302537.3817674/94‑1 (MQ=60)
    TCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACC                                                                                        <  SRR4302537.3754336/94‑1 (MQ=60)
    TCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACC                                                                                        <  SRR4302537.1859729/94‑1 (MQ=60)
     CGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCC                                                                                       <  SRR4302537.1969018/94‑1 (MQ=60)
       CCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGC                                                                                     <  SRR4302537.3531774/94‑1 (MQ=60)
       CCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGC                                                                                     >  SRR4302537.628709/1‑94 (MQ=60)
       CCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACAACCCGC                                                                                     <  SRR4302537.5826746/94‑1 (MQ=60)
       CCATCCCGATACTCATAATCACCCAGCCGGCGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACTCGC                                                                                     >  SRR4302537.4659694/1‑94 (MQ=60)
        CATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTGGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCC                                                                                    <  SRR4302537.3254950/94‑1 (MQ=60)
        CATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCC                                                                                    >  SRR4302537.2257069/1‑94 (MQ=60)
         ATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCG                                                                                   >  SRR4302537.6032945/1‑94 (MQ=60)
          TCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGG                                                                                  >  SRR4302537.3554275/1‑94 (MQ=60)
           CCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGG                                                                                 >  SRR4302537.547048/1‑94 (MQ=60)
           CCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGG                                                                                 <  SRR4302537.5575812/94‑1 (MQ=60)
           CCCGATACTCATAACCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGG                                                                                 <  SRR4302537.5675947/94‑1 (MQ=60)
            CCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGG                                                                                <  SRR4302537.3151642/94‑1 (MQ=60)
             CGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGT                                                                               >  SRR4302537.1472350/1‑94 (MQ=60)
             CGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGT                                                                               >  SRR4302537.4279679/1‑94 (MQ=60)
             CGATACTCATAATCACCCAGCCGGTGGCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGT                                                                               <  SRR4302537.5453917/94‑1 (MQ=60)
              GATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTC                                                                              >  SRR4302537.2465477/1‑94 (MQ=60)
              GATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTC                                                                              >  SRR4302537.2743933/1‑94 (MQ=60)
              GATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTC                                                                              <  SRR4302537.6154674/94‑1 (MQ=60)
               ATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCG                                                                             >  SRR4302537.6083413/1‑94 (MQ=60)
                TACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGA                                                                            >  SRR4302537.3552664/1‑94 (MQ=60)
                TACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGA                                                                            <  SRR4302537.3385087/94‑1 (MQ=60)
                 ACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA                                                                           <  SRR4302537.3575534/94‑1 (MQ=60)
                 ACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA                                                                           >  SRR4302537.5762440/1‑94 (MQ=60)
                  CTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAG                                                                          <  SRR4302537.1104320/94‑1 (MQ=60)
                   TCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAGC                                                                         >  SRR4302537.1862110/1‑94 (MQ=60)
                   TCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAGC                                                                         <  SRR4302537.1171369/94‑1 (MQ=60)
                    CATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAGcg                                                                        <  SRR4302537.2441302/94‑3 (MQ=60)
                    CATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAAGcg                                                                        >  SRR4302537.4170876/1‑92 (MQ=60)
                     ATAATCCCCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGT                                                                      <  SRR4302537.2463380/94‑1 (MQ=60)
                     ATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGT                                                                      >  SRR4302537.6438112/1‑94 (MQ=60)
                      TAATCCCCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTC                                                                     <  SRR4302537.5197573/94‑1 (MQ=60)
                      TAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTC                                                                     <  SRR4302537.4924711/94‑1 (MQ=60)
                       AATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCT                                                                    >  SRR4302537.5390357/1‑94 (MQ=60)
                       AATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCT                                                                    <  SRR4302537.2294331/94‑1 (MQ=60)
                       AACCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCT                                                                    <  SRR4302537.6007840/94‑1 (MQ=60)
                        ATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTG                                                                   <  SRR4302537.1253232/94‑1 (MQ=60)
                            CCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCCAT                                                               >  SRR4302537.5647645/1‑94 (MQ=60)
                            CCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAT                                                               <  SRR4302537.1482252/94‑1 (MQ=60)
                            CCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAAT                                                               <  SRR4302537.1165620/94‑1 (MQ=60)
                             CCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATG                                                              <  SRR4302537.2396366/94‑1 (MQ=60)
                              CAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAGTGA                                                             >  SRR4302537.1691078/1‑94 (MQ=60)
                               AGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGAC                                                            <  SRR4302537.2879356/94‑1 (MQ=60)
                                  CGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCC                                                         >  SRR4302537.3838822/1‑94 (MQ=60)
                                  CGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCC                                                         >  SRR4302537.2367632/1‑94 (MQ=60)
                                   GGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCT                                                        <  SRR4302537.1383785/94‑1 (MQ=60)
                                   GGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCT                                                        >  SRR4302537.3366034/1‑94 (MQ=60)
                                    GTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTT                                                       <  SRR4302537.3897773/94‑1 (MQ=60)
                                      GTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTA                                                     >  SRR4302537.2434385/1‑94 (MQ=60)
                                      GTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTA                                                     >  SRR4302537.5036578/1‑94 (MQ=60)
                                      GTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTA                                                     <  SRR4302537.2066172/94‑1 (MQ=60)
                                       TCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTAT                                                    <  SRR4302537.5736589/94‑1 (MQ=60)
                                        CAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATT                                                   >  SRR4302537.1489935/1‑94 (MQ=60)
                                         AGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTC                                                  <  SRR4302537.5305165/94‑1 (MQ=60)
                                         AGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTC                                                  <  SRR4302537.896907/94‑1 (MQ=60)
                                         AGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTC                                                  <  SRR4302537.4005152/94‑1 (MQ=60)
                                          GTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCG                                                 <  SRR4302537.929793/94‑1 (MQ=60)
                                          GTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCG                                                 >  SRR4302537.3119219/1‑94 (MQ=60)
                                          GTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCG                                                 <  SRR4302537.2590385/94‑1 (MQ=60)
                                          GTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCG                                                 >  SRR4302537.1259297/1‑94 (MQ=60)
                                           TACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGCCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGA                                                <  SRR4302537.193758/94‑1 (MQ=60)
                                           TACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGA                                                <  SRR4302537.4508179/94‑1 (MQ=60)
                                           TACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGA                                                >  SRR4302537.4121921/1‑94 (MQ=60)
                                            ACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGAT                                               >  SRR4302537.3228326/1‑94 (MQ=60)
                                            ACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGAT                                               >  SRR4302537.3230357/1‑94 (MQ=60)
                                             CTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATG                                              >  SRR4302537.6002713/1‑94 (MQ=60)
                                             CTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATG                                              <  SRR4302537.372952/94‑1 (MQ=60)
                                              TGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGC                                             <  SRR4302537.3371254/94‑1 (MQ=60)
                                              TGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGC                                             >  SRR4302537.4433964/1‑94 (MQ=60)
                                               GTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGTCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCA                                            >  SRR4302537.1964171/1‑94 (MQ=60)
                                               GTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCA                                            >  SRR4302537.3623676/1‑94 (MQ=60)
                                               GTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCA                                            >  SRR4302537.521323/1‑94 (MQ=60)
                                                 CGAATTTGATCGCTTCTCGCCACTCTCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAAT                                          >  SRR4302537.2118985/1‑94 (MQ=60)
                                                  GAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATT                                         <  SRR4302537.1418910/94‑1 (MQ=60)
                                                  GAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATT                                         <  SRR4302537.5349553/94‑1 (MQ=60)
                                                     TTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCC                                      <  SRR4302537.5612630/94‑1 (MQ=60)
                                                     TTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCC                                      <  SRR4302537.5216278/94‑1 (MQ=60)
                                                     TTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCC                                      >  SRR4302537.1265225/1‑94 (MQ=60)
                                                     TTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCC                                      <  SRR4302537.5746611/94‑1 (MQ=60)
                                                      TTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCA                                     >  SRR4302537.4659920/1‑94 (MQ=60)
                                                       TGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCAT                                    <  SRR4302537.5503105/94‑1 (MQ=60)
                                                       TGATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCAT                                    <  SRR4302537.626332/94‑1 (MQ=60)
                                                        GATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATA                                   <  SRR4302537.6101599/94‑1 (MQ=60)
                                                        GATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATA                                   <  SRR4302537.1804480/94‑1 (MQ=60)
                                                         ATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAA                                  >  SRR4302537.4064331/1‑94 (MQ=60)
                                                         ATCGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAA                                  >  SRR4302537.190391/1‑94 (MQ=60)
                                                           CGCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATT                                <  SRR4302537.1169932/94‑1 (MQ=60)
                                                            GCTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTT                               <  SRR4302537.4611373/94‑1 (MQ=60)
                                                             CTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTC                              >  SRR4302537.5593089/1‑94 (MQ=60)
                                                             CTTCTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTC                              >  SRR4302537.5861791/1‑94 (MQ=60)
                                                              TTCTCGCCGCTCGCTCTCACTCATTCCA‑GCACGCCCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCT                             <  SRR4302537.376585/94‑1 (MQ=60)
                                                                CTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCG                           <  SRR4302537.2571939/94‑1 (MQ=60)
                                                                CTCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCG                           <  SRR4302537.196991/94‑1 (MQ=60)
                                                                 TCGCCACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGC                          >  SRR4302537.2538369/1‑94 (MQ=60)
                                                                      CCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAAT                     <  SRR4302537.2676182/94‑1 (MQ=60)
                                                                      CCTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAAT                     <  SRR4302537.4711012/94‑1 (MQ=60)
                                                                      ACTCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAAT                     <  SRR4302537.555/94‑1 (MQ=60)
                                                                        TCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAT                   >  SRR4302537.6070768/1‑94 (MQ=60)
                                                                        TCGCTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATAT                   <  SRR4302537.4090554/94‑1 (MQ=60)
                                                                           CTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTT                >  SRR4302537.4829020/1‑94 (MQ=60)
                                                                           CTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTT                <  SRR4302537.6007969/94‑1 (MQ=60)
                                                                           CTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTT                >  SRR4302537.1612815/1‑94 (MQ=60)
                                                                           CTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTT                >  SRR4302537.5642979/1‑94 (MQ=60)
                                                                           CTCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTT                >  SRR4302537.4469496/1‑94 (MQ=60)
                                                                            TCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTG               >  SRR4302537.1239886/1‑94 (MQ=60)
                                                                            TCTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTG               >  SRR4302537.6313021/1‑94 (MQ=60)
                                                                             CTCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGT              >  SRR4302537.3443815/1‑94 (MQ=60)
                                                                              TCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTA             <  SRR4302537.1785974/94‑1 (MQ=60)
                                                                              TCACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTA             >  SRR4302537.1017056/1‑94 (MQ=60)
                                                                               CACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGCCGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAG            <  SRR4302537.4150286/94‑1 (MQ=60)
                                                                               CACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAG            <  SRR4302537.2631685/94‑1 (MQ=60)
                                                                               CACTCATGCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAG            <  SRR4302537.5250502/94‑1 (MQ=60)
                                                                                ACTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGG           >  SRR4302537.2311898/1‑94 (MQ=60)
                                                                                 CTCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGG          <  SRR4302537.3906159/94‑1 (MQ=60)
                                                                                  TCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGC         <  SRR4302537.3424406/94‑1 (MQ=60)
                                                                                  TCATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGC         <  SRR4302537.50894/94‑1 (MQ=60)
                                                                                   CATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCT        >  SRR4302537.1568781/1‑94 (MQ=60)
                                                                                   CATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCT        <  SRR4302537.288166/94‑1 (MQ=60)
                                                                                   CATTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCT        <  SRR4302537.2928072/94‑1 (MQ=60)
                                                                                     TTCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTA      <  SRR4302537.167525/94‑1 (MQ=60)
                                                                                      TCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTAT     <  SRR4302537.4989705/94‑1 (MQ=60)
                                                                                      TCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTAT     <  SRR4302537.796566/94‑1 (MQ=60)
                                                                                      TCCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTAT     >  SRR4302537.4495065/1‑94 (MQ=60)
                                                                                       CCA‑GCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATT    <  SRR4302537.4175973/94‑1 (MQ=60)
                                                                                          AGCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAT  <  SRR4302537.5104833/94‑1 (MQ=60)
                                                                                          AGCACGACCCGCCGGGGTCGAA‑GCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAT  <  SRR4302537.519685/94‑1 (MQ=60)
                                                                                           |                                                                                             
CCAATCGCCATCCCGATACTCATAATCACCCAGCCGGTGTCAGTACTGTCGAATTTGATCGCTTCTCGCCACTCGCTCTCACTCATTCCAGGCACGACCCGCCGGGGTCGAAGGCGTCTGCAATGACGCCTTTATTCGATGCAATTTCCATAATTTCTCGCTCAATATTTTGTAGGGCTTATTAT  >  NC_000913/3255503‑3255687

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑