Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,352,664 T→C V133A (GTT→GCT)  gltB → glutamate synthase large subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,352,6640TC97.7% 718.9 / 4.2 216V133A (GTT→GCT) gltBglutamate synthase large subunit
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base C (121/90);  total (121/95)
Fisher's exact test for biased strand distribution p-value = 1.55e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.85e-01

Only 100 of 219 total aligned reads displayed.

GGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCT  >  NC_000913/3352572‑3352753
                                                                                            |                                                                                         
 gAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGtt                                                                                          <  1:3902713/93‑1 (MQ=255)
    cgcgGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTgaa                                                                                       <  1:931651/93‑1 (MQ=255)
     gcgGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTgaag                                                                                      >  2:576989/1‑93 (MQ=255)
     gcgGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCACACGCCGCATCGCTgaag                                                                                      >  2:5105437/1‑93 (MQ=255)
      cgGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTgaaga                                                                                     <  1:1667247/93‑1 (MQ=255)
      cgGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTgaaga                                                                                     >  1:255860/1‑93 (MQ=255)
       gggtGGCGTTTAGCAAAAAACTCCGCTGACGGAATGCTCTTCCTGAATAAAGATCCTGAACTCGCAGCTGCCGCACGCCGCATCGCTgaagaa                                                                                    <  2:276893/90‑1 (MQ=255)
       ggCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTgaagaa                                                                                    <  2:3101581/93‑1 (MQ=255)
       ggCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTgaagaa                                                                                    <  2:193949/93‑1 (MQ=255)
       ggCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTgaagaa                                                                                    >  1:4131899/1‑93 (MQ=255)
       gacTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTgaagaa                                                                                    <  2:4805756/91‑1 (MQ=255)
        gCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTgaagaag                                                                                   <  2:2469283/93‑1 (MQ=255)
        gCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTgaagaag                                                                                   >  2:1610566/1‑93 (MQ=255)
          tGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTgaagaagaa                                                                                 >  2:5533330/1‑93 (MQ=255)
           ggCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAAc                                                                                >  2:4831676/1‑93 (MQ=255)
           ggCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAAc                                                                                >  1:66155/1‑93 (MQ=255)
            gCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACt                                                                               >  2:644164/1‑93 (MQ=255)
              gTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGc                                                                             >  1:4985229/1‑93 (MQ=255)
                ttAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGACCTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCaa                                                                           <  2:5759081/93‑1 (MQ=255)
                ttAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCaa                                                                           >  2:155850/1‑93 (MQ=255)
                ttAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCaa                                                                           <  2:3373457/93‑1 (MQ=255)
                   gCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAAcgc                                                                        >  2:3836687/1‑93 (MQ=255)
                      aaaaaCTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGaa                                                                     <  2:2094257/93‑1 (MQ=255)
                      aaaaaCTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGaa                                                                     <  1:2445583/93‑1 (MQ=255)
                         aaCTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAAcc                                                                  >  2:2438361/1‑93 (MQ=255)
                         aaCTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAAcc                                                                  <  1:4436952/93‑1 (MQ=255)
                         aaCTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAAcc                                                                  >  1:4855984/1‑93 (MQ=255)
                          gcTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCt                                                                 >  2:6209540/2‑93 (MQ=255)
                          aCTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCt                                                                 >  2:5816777/1‑93 (MQ=255)
                           cTACGCTGTCGGGATTCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCtt                                                                <  2:665918/93‑1 (MQ=255)
                           cTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCtt                                                                <  2:1392210/93‑1 (MQ=255)
                           cTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGAGAAACCtt                                                                >  2:602994/1‑93 (MQ=255)
                            tACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTg                                                               >  2:844300/1‑93 (MQ=255)
                            tACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTg                                                               >  2:4767709/1‑93 (MQ=255)
                             aCGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGt                                                              >  2:5368187/1‑93 (MQ=255)
                             aCGCTGTCGGGATGCTCTCCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGt                                                              <  1:3486429/93‑1 (MQ=255)
                               gCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCg                                                            >  2:2678632/1‑93 (MQ=255)
                                  gTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGAtt                                                         >  2:5019334/1‑93 (MQ=255)
                                   tCGGGATGCTCTTCCTGAATAAAGATCCTGACCTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATtg                                                        <  1:5689329/93‑1 (MQ=255)
                                   tCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATtg                                                        >  1:5006557/1‑93 (MQ=255)
                                   tCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATtg                                                        >  2:2300635/1‑93 (MQ=255)
                                     gggATGCTCTTCGTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATtgtg                                                      <  2:5456447/93‑1 (MQ=255)
                                     gggATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATtgtg                                                      <  1:1275547/93‑1 (MQ=255)
                                     gggATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATtgtg                                                      >  1:3046096/1‑93 (MQ=255)
                                     gggATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATtgtg                                                      >  1:978437/1‑93 (MQ=255)
                                      ggATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTgg                                                     <  1:3221914/93‑1 (MQ=255)
                                       gATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTggg                                                    >  1:103698/1‑93 (MQ=255)
                                        aTGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGc                                                   <  1:4486420/93‑1 (MQ=255)
                                         tGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCt                                                  <  1:1141288/93‑1 (MQ=255)
                                         tGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCt                                                  >  2:5386431/1‑93 (MQ=255)
                                         tGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCt                                                  >  1:5613207/1‑93 (MQ=255)
                                         tGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCt                                                  >  2:3616030/1‑93 (MQ=255)
                                          gCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTg                                                 >  2:2404666/1‑93 (MQ=255)
                                            tctTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGc                                               <  1:3414833/93‑1 (MQ=255)
                                              ttCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGt                                             <  1:6202915/93‑1 (MQ=255)
                                               tCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTg                                            >  2:5280322/1‑93 (MQ=255)
                                               tCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTg                                            >  2:3543346/1‑93 (MQ=255)
                                                ccTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGa                                           <  1:2411925/93‑1 (MQ=255)
                                                 cTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGAt                                          <  2:4981701/93‑1 (MQ=255)
                                                  tGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATg                                         >  1:4869829/1‑93 (MQ=255)
                                                   gAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGt                                        >  2:3173703/1‑93 (MQ=255)
                                                   gAATAAAGATCCTGAACTCGCCGCTGCCGAACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGt                                        >  2:3491422/1‑93 (MQ=255)
                                                    aaTAAAGATCCTGACCTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTc                                       <  2:3428191/93‑1 (MQ=255)
                                                    aaTAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTc                                       >  2:4302126/1‑93 (MQ=255)
                                                      tAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTccc                                     >  2:3674819/1‑93 (MQ=255)
                                                      tAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTccc                                     >  1:579100/1‑93 (MQ=255)
                                                         aGATCCTGATCTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCAc                                  <  1:6041430/93‑1 (MQ=255)
                                                         aGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCAc                                  <  1:897548/93‑1 (MQ=255)
                                                         aGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCAc                                  >  2:400057/1‑93 (MQ=255)
                                                          gATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACt                                 >  1:2967256/1‑93 (MQ=255)
                                                           aTCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAAGCTTGTCGATTGTGGGCTGGCGTGATGTCACCACTa                                >  1:2786587/1‑93 (MQ=255)
                                                            tCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTaa                               <  2:3310851/93‑1 (MQ=255)
                                                             ccTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAAc                              >  1:366042/1‑93 (MQ=255)
                                                               tGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGa                            >  2:4506692/1‑93 (MQ=255)
                                                                gAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGaa                           <  1:1354769/93‑1 (MQ=255)
                                                                 aaCTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAg                          >  1:179724/1‑93 (MQ=255)
                                                                 aaCTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAg                          >  1:1874104/1‑93 (MQ=255)
                                                                  aCTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAgg                         <  1:1957448/93‑1 (MQ=255)
                                                                  aCTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAgg                         <  1:4088734/93‑1 (MQ=255)
                                                                   cTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGc                        >  1:4028785/1‑93 (MQ=255)
                                                                     cgccgcTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGt                      <  1:3614404/93‑1 (MQ=255)
                                                                       ccgcTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGc                    >  2:1292438/1‑93 (MQ=255)
                                                                       ccgcTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGc                    <  1:2893581/93‑1 (MQ=255)
                                                                        cgcTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCt                   <  2:203269/93‑1 (MQ=255)
                                                                         gcTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTg                  >  1:4742702/1‑93 (MQ=255)
                                                                         gcTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTg                  >  2:2572152/1‑93 (MQ=255)
                                                                            gCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGt               <  2:1641638/93‑1 (MQ=255)
                                                                            gCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGt               <  1:5254742/93‑1 (MQ=255)
                                                                             ccGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTg              <  1:5091872/93‑1 (MQ=255)
                                                                              cGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGa             >  1:2639760/1‑93 (MQ=255)
                                                                               gCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGaa            >  2:6230937/1‑93 (MQ=255)
                                                                                 aCGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAAt          <  1:3638863/93‑1 (MQ=255)
                                                                                 aCGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAAt          <  2:2146542/93‑1 (MQ=255)
                                                                                  cgccgcATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATc         <  2:4150342/93‑1 (MQ=255)
                                                                                      gcATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGccc     >  1:4667999/1‑93 (MQ=255)
                                                                                       cATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGGAGTCGCcct    >  2:5601287/1‑93 (MQ=255)
                                                                                       cATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcct    >  1:5470906/1‑93 (MQ=255)
                                                                                        aTCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctc   >  1:4228203/1‑93 (MQ=255)
                                                                                        aTCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctc   >  1:4494843/1‑93 (MQ=255)
                                                                                         tCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCcctct  <  2:4355215/93‑1 (MQ=255)
                                                                                            |                                                                                         
GGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCT  >  NC_000913/3352572‑3352753

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTC  >  NC_000913/3352571‑3352757
                                                                                             |                                                                                             
AGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGT                                                                                               >  SRR4302537.711305/1‑94 (MQ=60)
AGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGC                                                                                               >  SRR4302537.2280159/1‑94 (MQ=60)
 GGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCT                                                                                              <  SRR4302537.1184882/94‑1 (MQ=60)
 GGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCT                                                                                              <  SRR4302537.3200004/94‑1 (MQ=60)
 GGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCT                                                                                              <  SRR4302537.6038090/94‑1 (MQ=60)
 GGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAAATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCT                                                                                              <  SRR4302537.3106671/94‑1 (MQ=60)
  GAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTG                                                                                             <  SRR4302537.3929998/94‑1 (MQ=60)
     CGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAG                                                                                          <  SRR4302537.938076/94‑1 (MQ=60)
     CGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAG                                                                                          <  SRR4302537.4863834/94‑1 (MQ=60)
      GCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGA                                                                                         >  SRR4302537.257678/1‑94 (MQ=60)
       CGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACTCCGCATCGCTGAAGAA                                                                                        <  SRR4302537.2437249/94‑1 (MQ=60)
       CGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAA                                                                                        <  SRR4302537.1678923/94‑1 (MQ=60)
       CGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAA                                                                                        >  SRR4302537.4160734/1‑94 (MQ=60)
       CGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAA                                                                                        <  SRR4302537.264408/94‑1 (MQ=60)
           TGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAAC                                                                                    <  SRR4302537.4607102/94‑1 (MQ=60)
           TGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAAC                                                                                    >  SRR4302537.66644/1‑94 (MQ=60)
            GGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACT                                                                                   <  SRR4302537.345984/94‑1 (MQ=60)
            GGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACT                                                                                   >  SRR4302537.914352/1‑94 (MQ=60)
             GCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTG                                                                                  >  SRR4302537.486619/1‑94 (MQ=60)
              CGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGCAGAAGAACTGC                                                                                 >  SRR4302537.2728782/1‑94 (MQ=60)
              CGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGC                                                                                 >  SRR4302537.5019590/1‑94 (MQ=60)
                TTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAA                                                                               >  SRR4302537.3992978/1‑94 (MQ=60)
                TTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAA                                                                               <  SRR4302537.3912820/94‑1 (MQ=60)
                TTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAA                                                                               <  SRR4302537.3603391/94‑1 (MQ=60)
                TTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAA                                                                               >  SRR4302537.5189805/1‑94 (MQ=60)
                TTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAA                                                                               >  SRR4302537.1054564/1‑94 (MQ=60)
                 TTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTTAAGAAGAACTGCAAC                                                                              <  SRR4302537.4986279/94‑1 (MQ=60)
                  TAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACG                                                                             <  SRR4302537.4485409/94‑1 (MQ=60)
                  TAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACG                                                                             <  SRR4302537.3369106/94‑1 (MQ=60)
                  TAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACG                                                                             <  SRR4302537.1904989/94‑1 (MQ=60)
                    GCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCG                                                                           <  SRR4302537.6262933/94‑1 (MQ=60)
                     CAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGA                                                                          >  SRR4302537.2973110/1‑94 (MQ=60)
                      AAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGCAGAAGAACTGCAACGCGAA                                                                         >  SRR4302537.564585/1‑94 (MQ=60)
                       AAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAA                                                                        <  SRR4302537.2462792/94‑1 (MQ=60)
                         AAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACC                                                                      >  SRR4302537.4889489/1‑94 (MQ=60)
                          AACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCT                                                                     >  SRR4302537.5426117/1‑94 (MQ=60)
                          AACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCT                                                                     <  SRR4302537.4467586/94‑1 (MQ=60)
                           ACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTT                                                                    <  SRR4302537.6215850/94‑1 (MQ=60)
                            CTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTG                                                                   <  SRR4302537.4150900/94‑1 (MQ=60)
                            CTACGCTGTCGGGAGGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTG                                                                   <  SRR4302537.957089/94‑1 (MQ=60)
                             TACGCTGTCGGGATGCTCTTCCTGAATAAAGTTCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGT                                                                  >  SRR4302537.1307609/1‑94 (MQ=60)
                             TACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGT                                                                  >  SRR4302537.1571622/1‑94 (MQ=60)
                             TACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGT                                                                  >  SRR4302537.5035711/1‑94 (MQ=60)
                              ACGCTGTCGGGATGCTCTCCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTC                                                                 <  SRR4302537.3511086/94‑1 (MQ=60)
                                GCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGA                                                               >  SRR4302537.3082674/1‑94 (MQ=60)
                                  TGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATT                                                             <  SRR4302537.2265840/94‑1 (MQ=60)
                                   GTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTG                                                            >  SRR4302537.5041038/1‑94 (MQ=60)
                                    TCGGGATGCTCTTCCTGAATAAAGATCCTGACCTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGT                                                           <  SRR4302537.5728245/94‑1 (MQ=60)
                                    GCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGT                                                           <  SRR4302537.1703140/94‑1 (MQ=60)
                                     CGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTG                                                          >  SRR4302537.3067319/1‑94 (MQ=60)
                                     CGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTG                                                          >  SRR4302537.985190/1‑94 (MQ=60)
                                      GGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGG                                                         <  SRR4302537.1284418/94‑1 (MQ=60)
                                       GGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGG                                                        <  SRR4302537.3244586/94‑1 (MQ=60)
                                       GGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGG                                                        >  SRR4302537.104445/1‑94 (MQ=60)
                                        GATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGC                                                       <  SRR4302537.1867430/94‑1 (MQ=60)
                                         ATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCT                                                      <  SRR4302537.4517457/94‑1 (MQ=60)
                                         ATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCT                                                      >  SRR4302537.5651586/1‑94 (MQ=60)
                                         ATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCT                                                      >  SRR4302537.3217043/1‑94 (MQ=60)
                                          TGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTG                                                     <  SRR4302537.1149217/94‑1 (MQ=60)
                                             TCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCG                                                  <  SRR4302537.3439011/94‑1 (MQ=60)
                                               TTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTG                                                <  SRR4302537.6245667/94‑1 (MQ=60)
                                                TCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGA                                               >  SRR4302537.2862989/1‑94 (MQ=60)
                                                 CCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGAT                                              <  SRR4302537.6114003/94‑1 (MQ=60)
                                                 CCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGAT                                              <  SRR4302537.4347934/94‑1 (MQ=60)
                                                 CCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGAT                                              <  SRR4302537.2428905/94‑1 (MQ=60)
                                                  CTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATG                                             >  SRR4302537.4903438/1‑94 (MQ=60)
                                                  CTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATG                                             <  SRR4302537.2512510/94‑1 (MQ=60)
                                                     AATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCC                                          >  SRR4302537.583426/1‑94 (MQ=60)
                                                      ATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCC                                         >  SRR4302537.583064/1‑94 (MQ=60)
                                                       TAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCC                                        >  SRR4302537.5214384/1‑94 (MQ=60)
                                                        AAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCA                                       >  SRR4302537.378207/1‑94 (MQ=60)
                                                        AAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCA                                       <  SRR4302537.4896814/94‑1 (MQ=60)
                                                          AGATCCTGATCTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACT                                     <  SRR4302537.6082930/94‑1 (MQ=60)
                                                          AGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACT                                     <  SRR4302537.903739/94‑1 (MQ=60)
                                                          AGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACT                                     >  SRR4302537.2988067/1‑94 (MQ=60)
                                                           GATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAAGCTTGTCGATTGTGGGCTGGCGTGATGTCACCACTA                                    >  SRR4302537.2806072/1‑94 (MQ=60)
                                                           GATCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTA                                    >  SRR4302537.5815159/1‑94 (MQ=60)
                                                             TCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAAC                                  >  SRR4302537.368584/1‑94 (MQ=60)
                                                             TCCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAAC                                  <  SRR4302537.5098417/94‑1 (MQ=60)
                                                              CCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACG                                 >  SRR4302537.2656706/1‑94 (MQ=60)
                                                              CCTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACG                                 <  SRR4302537.3662088/94‑1 (MQ=60)
                                                               CTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTCACGA                                >  SRR4302537.570623/1‑94 (MQ=60)
                                                               CTGAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGA                                >  SRR4302537.4064839/1‑94 (MQ=60)
                                                                 GAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAG                              <  SRR4302537.1364211/94‑1 (MQ=60)
                                                                 GAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAG                              >  SRR4302537.1887145/1‑94 (MQ=60)
                                                                 GAACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAG                              >  SRR4302537.181003/1‑94 (MQ=60)
                                                                   ACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGC                            >  SRR4302537.4056986/1‑94 (MQ=60)
                                                                   ACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGC                            <  SRR4302537.4117286/94‑1 (MQ=60)
                                                                   ACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGC                            <  SRR4302537.4522098/94‑1 (MQ=60)
                                                                   ACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGC                            >  SRR4302537.301492/1‑94 (MQ=60)
                                                                   ACTCGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGC                            <  SRR4302537.1971067/94‑1 (MQ=60)
                                                                      CGCCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTG                         <  SRR4302537.3639882/94‑1 (MQ=60)
                                                                        CCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCT                       <  SRR4302537.2913892/94‑1 (MQ=60)
                                                                        CCGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCT                       <  SRR4302537.422014/94‑1 (MQ=60)
                                                                         CGCTGCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTG                      >  SRR4302537.4775478/1‑94 (MQ=60)
                                                                             GCCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTG                  <  SRR4302537.5290901/94‑1 (MQ=60)
                                                                              CCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGA                 >  SRR4302537.2658232/1‑94 (MQ=60)
                                                                              CCGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGA                 <  SRR4302537.5126933/94‑1 (MQ=60)
                                                                               CGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAA                >  SRR4302537.1169563/1‑94 (MQ=60)
                                                                               CGCACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAA                >  SRR4302537.1835265/1‑94 (MQ=60)
                                                                                  ACGCCGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATC             <  SRR4302537.3664485/94‑1 (MQ=60)
                                                                                      CGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCC         >  SRR4302537.4700291/1‑94 (MQ=60)
                                                                                      CGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCC         >  SRR4302537.3666424/1‑94 (MQ=60)
                                                                                      CGCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTATGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCC         >  SRR4302537.4744328/1‑94 (MQ=60)
                                                                                       GCATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCT        >  SRR4302537.5508229/1‑94 (MQ=60)
                                                                                        CATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTC       >  SRR4302537.4257643/1‑94 (MQ=60)
                                                                                        CATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTC       >  SRR4302537.4525935/1‑94 (MQ=60)
                                                                                         ATCGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCT      >  SRR4302537.4445603/1‑94 (MQ=60)
                                                                                           CGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCC    >  SRR4302537.1224526/1‑94 (MQ=60)
                                                                                           CGCTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCC    >  SRR4302537.2494495/1‑94 (MQ=60)
                                                                                             CTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTC  >  SRR4302537.3837533/1‑94 (MQ=60)
                                                                                             |                                                                                             
AGGAGCGCGGCTGGCGTTTAGCAAAAAACTACGCTGTCGGGATGCTCTTCCTGAATAAAGATCCTGAACTCGCCGCTGCCGCACGCCGCATCGTTGAAGAAGAACTGCAACGCGAAACCTTGTCGATTGTGGGCTGGCGTGATGTCCCCACTAACGAAGGCGTGCTGGGTGAAATCGCCCTCTCCTC  >  NC_000913/3352571‑3352757

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑