Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,374,875	T→G	T61P (ACC→CCC)	sspA ←	stringent starvation protein A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,374,875	0	T	G	99.6%	881.0 / ‑5.1	252	T61P (ACC→CCC)	sspA	stringent starvation protein A
Reads supporting (aligned to +/- strand): ref base T (0/1); new base G (132/119); total (132/120)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 258 total aligned reads displayed.

AGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT  >  NC_000913/3374786‑3374964

aGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCgg                                                                                        <  2:6243945/93‑1 (MQ=255)

     tCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGc                                                                                   >  1:603192/1‑93 (MQ=255)

       aGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGctc                                                                                 <  2:205396/93‑1 (MQ=255)

       aGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGctc                                                                                 <  1:2535293/93‑1 (MQ=255)

        gTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGctct                                                                                >  1:5284894/1‑93 (MQ=255)

        gTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGctct                                                                                <  2:1873055/93‑1 (MQ=255)

          ggCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGa                                                                              <  1:4532910/93‑1 (MQ=255)

           gCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGAt                                                                             >  1:5129387/1‑93 (MQ=255)

           gCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGAt                                                                             <  1:885309/93‑1 (MQ=255)

            gggATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGAtt                                                                            <  1:4686816/92‑1 (MQ=255)

            cGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGAtt                                                                            <  1:2704699/93‑1 (MQ=255)

              gATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCg                                                                          >  2:5423764/1‑93 (MQ=255)

               aTGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCgg                                                                         <  2:336068/93‑1 (MQ=255)

                tGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCggg                                                                        >  1:656035/1‑93 (MQ=255)

                tGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCggg                                                                        <  1:3918178/93‑1 (MQ=255)

                tGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCggg                                                                        >  2:1513653/1‑93 (MQ=255)

                   gggAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTg                                                                     >  1:2394305/1‑93 (MQ=255)

                    ggAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGa                                                                    <  2:2811423/93‑1 (MQ=255)

                    ggAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGa                                                                    <  1:2181645/93‑1 (MQ=255)

                    ggAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGa                                                                    <  1:2029905/93‑1 (MQ=255)

                    ggAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGa                                                                    <  2:5741879/93‑1 (MQ=255)

                     gAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAg                                                                   >  1:5377880/1‑93 (MQ=255)

                     gAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAg                                                                   <  2:6163370/93‑1 (MQ=255)

                     gAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAg                                                                   <  2:557550/93‑1 (MQ=255)

                     gAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAg                                                                   <  1:6098319/93‑1 (MQ=255)

                      aaaCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAgg                                                                  <  1:2924062/93‑1 (MQ=255)

                        aCGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTc                                                                >  1:5819968/1‑93 (MQ=255)

                         cGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCa                                                               >  2:5286188/1‑93 (MQ=255)

                          gCTCATCCAGATATGCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCaa                                                              <  2:4749062/93‑1 (MQ=255)

                           cTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAAt                                                             >  1:4158492/1‑93 (MQ=255)

                           cTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAAt                                                             <  2:4507431/93‑1 (MQ=255)

                           cTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCACGGGCGGAACGCTCTGATTCGGGTTGAGGTCAAt                                                             >  2:15624/1‑93 (MQ=255)

                            tCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATc                                                            >  2:3350808/1‑93 (MQ=255)

                             cATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCa                                                           <  2:2262921/93‑1 (MQ=255)

                             cATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCa                                                           >  2:4670384/1‑93 (MQ=255)

                               tCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGa                                                         >  1:1747398/1‑93 (MQ=255)

                               tCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGa                                                         >  2:3790899/1‑93 (MQ=255)

                                ccAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGAt                                                        <  1:3473505/93‑1 (MQ=255)

                                  aGATTTTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATcc                                                      <  2:5790870/93‑1 (MQ=255)

                                  aGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATcc                                                      <  1:4449638/93‑1 (MQ=255)

                                  aGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATcc                                                      >  2:215080/1‑93 (MQ=255)

                                  aGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATcc                                                      <  1:1592278/93‑1 (MQ=255)

                                  aGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATcc                                                      >  1:95594/1‑93 (MQ=255)

                                   gATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCt                                                     >  1:3555081/1‑93 (MQ=255)

                                   gATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCt                                                     >  1:2866862/1‑93 (MQ=255)

                                   gATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCt                                                     >  1:6293621/1‑93 (MQ=255)

                                   gATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCt                                                     <  2:3917217/93‑1 (MQ=255)

                                    atatTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTg                                                    >  2:2750719/1‑93 (MQ=255)

                                     tatTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGa                                                   >  1:116129/1‑93 (MQ=255)

                                     tatTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGa                                                   <  2:3524332/93‑1 (MQ=255)

                                     tatTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGa                                                   <  1:5327352/93‑1 (MQ=255)

                                     tatTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGa                                                   <  1:1674496/93‑1 (MQ=255)

                                      atTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAg                                                  >  2:4050475/1‑93 (MQ=255)

                                       ttCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAgg                                                 >  2:5929945/1‑93 (MQ=255)

                                          cATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCgg                                              >  2:4887315/1‑93 (MQ=255)

                                          cATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCgg                                              >  1:5534778/1‑93 (MQ=255)

                                          cATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCgg                                              >  2:1704501/1‑93 (MQ=255)

                                           aTAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGa                                             >  2:3251290/1‑93 (MQ=255)

                                            tAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAt                                            >  2:6041645/1‑93 (MQ=255)

                                            tAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAt                                            >  2:5831219/1‑93 (MQ=255)

                                            tAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCAGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAt                                            <  1:4174820/93‑1 (MQ=255)

                                             aaTGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAtt                                           <  1:683985/93‑1 (MQ=255)

                                             aaTGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAtt                                           >  1:2229978/1‑93 (MQ=255)

                                               tgatgCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGt                                         >  2:3823764/1‑93 (MQ=255)

                                                  tccGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCt                                      <  1:2633386/91‑1 (MQ=255)

                                                      agaTTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTc                                  >  2:4340672/1‑93 (MQ=255)

                                                       gaTTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTcc                                 <  2:4595333/93‑1 (MQ=255)

                                                       gaTTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTcc                                 <  2:1120656/93‑1 (MQ=255)

                                                        aTTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCa                                <  1:457833/93‑1 (MQ=255)

                                                        aTTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCa                                <  2:2108604/93‑1 (MQ=255)

                                                         ttCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCAc                               <  1:3605583/93‑1 (MQ=255)

                                                            ccACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgtg                            >  1:4982655/1‑93 (MQ=255)

                                                             cacaGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgtgt                           >  2:5648296/1‑93 (MQ=255)

                                                             cacaGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACgtgt                           >  1:4637412/1‑93 (MQ=255)

                                                              acaGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGtt                          >  2:1223891/1‑93 (MQ=255)

                                                                aGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCg                        >  2:853195/1‑93 (MQ=255)

                                                                 gggTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGa                       >  1:3079009/1‑93 (MQ=255)

                                                                 gggTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGa                       <  1:3380722/93‑1 (MQ=255)

                                                                 gggTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGa                       >  2:220668/1‑93 (MQ=255)

                                                                 gggTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGa                       >  2:5239190/1‑93 (MQ=255)

                                                                   gTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtc                     <  2:3461773/93‑1 (MQ=255)

                                                                   gTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtc                     <  2:1651909/93‑1 (MQ=255)

                                                                    tCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtct                    <  2:493905/93‑1 (MQ=255)

                                                                     cAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtctc                   <  2:658272/93‑1 (MQ=255)

                                                                     cAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAtctc                   >  1:4062914/1‑93 (MQ=255)

                                                                       gCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTCGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGa                 >  1:1402040/1‑93 (MQ=255)

                                                                         tCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGaaa               >  2:3010738/1‑93 (MQ=255)

                                                                          cACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAAc              <  2:2141422/93‑1 (MQ=255)

                                                                           aCGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACt             >  1:3646237/1‑93 (MQ=255)

                                                                             gATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTa           >  2:5054701/1‑93 (MQ=255)

                                                                             gATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTa           <  1:3320174/93‑1 (MQ=255)

                                                                              gtCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTac          <  2:3553643/92‑1 (MQ=255)

                                                                                ccaccaGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTacac        <  1:1856310/93‑1 (MQ=255)

                                                                                  accaGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCt      <  1:575043/93‑1 (MQ=255)

                                                                                   ccaGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCtt     >  1:1033104/1‑93 (MQ=255)

                                                                                     aGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtc   >  2:2830771/1‑93 (MQ=255)

                                                                                     aGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtc   <  2:645856/93‑1 (MQ=255)

                                                                                     aGGGGCGGAACGCGCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtc   <  1:5442523/93‑1 (MQ=255)

                                                                                      ggggCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtct  >  1:3098551/1‑93 (MQ=255)

                                                                                      ggggCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTtct  >  2:1317395/1‑93 (MQ=255)

AGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT  >  NC_000913/3374786‑3374964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAG  >  NC_000913/3374782‑3374967

AAAGAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGG                                                                                              <  SRR4302537.5852492/94‑1 (MQ=60)

AAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGG                                                                                              <  SRR4302537.4135960/94‑1 (MQ=60)

AAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATACACCAGGGG                                                                                              <  SRR4302537.2976394/94‑1 (MQ=60)

    AGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGA                                                                                          >  SRR4302537.3379055/1‑94 (MQ=60)

    AGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGA                                                                                          >  SRR4302537.3380973/1‑94 (MQ=60)

      GCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAAC                                                                                        <  SRR4302537.3018792/94‑1 (MQ=60)

        ATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGC                                                                                      >  SRR4302537.607318/1‑94 (MQ=60)

           AGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCT                                                                                   >  SRR4302537.5321237/1‑94 (MQ=60)

           AGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCT                                                                                   <  SRR4302537.2553084/94‑1 (MQ=60)

           AGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCT                                                                                   <  SRR4302537.4563056/94‑1 (MQ=60)

           AGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCT                                                                                   <  SRR4302537.3079891/94‑1 (MQ=60)

              GGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGAT                                                                                <  SRR4302537.4564300/94‑1 (MQ=60)

              GGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGAT                                                                                >  SRR4302537.5164736/1‑94 (MQ=60)

               GCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATT                                                                               <  SRR4302537.891405/94‑1 (MQ=60)

                GGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTC                                                                              <  SRR4302537.4719241/94‑1 (MQ=60)

                CGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTC                                                                              <  SRR4302537.2723580/94‑1 (MQ=60)

                 GGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCG                                                                             >  SRR4302537.41560/1‑94 (MQ=60)

                  GATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGG                                                                            >  SRR4302537.1233063/1‑94 (MQ=60)

                   ATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGG                                                                           >  SRR4302537.660507/1‑94 (MQ=60)

                   ATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGG                                                                           <  SRR4302537.1914629/94‑1 (MQ=60)

                    TGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGT                                                                          <  SRR4302537.3945567/94‑1 (MQ=60)

                    TGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGT                                                                          <  SRR4302537.3607349/94‑1 (MQ=60)

                      CGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTG                                                                        >  SRR4302537.2411167/1‑94 (MQ=60)

                        GGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACATGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAG                                                                      <  SRR4302537.2795715/94‑1 (MQ=60)

                        GGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAG                                                                      >  SRR4302537.5414668/1‑94 (MQ=60)

                        GGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAG                                                                      <  SRR4302537.2197012/94‑1 (MQ=60)

                        GGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAG                                                                      <  SRR4302537.2044057/94‑1 (MQ=60)

                         GAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGG                                                                     <  SRR4302537.6140226/94‑1 (MQ=60)

                         GAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGG                                                                     <  SRR4302537.2431926/94‑1 (MQ=60)

                         GAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGG                                                                     >  SRR4302537.4089164/1‑94 (MQ=60)

                          AAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGT                                                                    >  SRR4302537.366827/1‑94 (MQ=60)

                          AAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGT                                                                    <  SRR4302537.2944601/94‑1 (MQ=60)

                           AACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTC                                                                   >  SRR4302537.767009/1‑94 (MQ=60)

                           AACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTC                                                                   >  SRR4302537.5859855/1‑94 (MQ=60)

                             CGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAA                                                                 >  SRR4302537.190843/1‑94 (MQ=60)

                              GCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAAT                                                                >  SRR4302537.1187293/1‑94 (MQ=60)

                              GCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAAT                                                                >  SRR4302537.4187531/1‑94 (MQ=60)

                               CTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATC                                                               <  SRR4302537.6109916/94‑1 (MQ=60)

                               CTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATC                                                               >  SRR4302537.1617118/1‑94 (MQ=60)

                                 CATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAG                                                             <  SRR4302537.5631741/94‑1 (MQ=60)

                                  ATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGA                                                            >  SRR4302537.2693847/1‑94 (MQ=60)

                                  ATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGA                                                            >  SRR4302537.2691773/1‑94 (MQ=60)

                                  ATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGA                                                            >  SRR4302537.1759588/1‑94 (MQ=60)

                                  ATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGA                                                            >  SRR4302537.924145/1‑94 (MQ=60)

                                    CCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATC                                                          <  SRR4302537.3498084/94‑1 (MQ=60)

                                    CCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATC                                                          >  SRR4302537.3512527/1‑94 (MQ=60)

                                     CAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCC                                                         >  SRR4302537.96293/1‑94 (MQ=60)

                                     CAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCC                                                         >  SRR4302537.6506264/1‑94 (MQ=60)

                                      AGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCT                                                        <  SRR4302537.4199995/94‑1 (MQ=60)

                                      AGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCT                                                        <  SRR4302537.4480368/94‑1 (MQ=60)

                                      AGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCT                                                        <  SRR4302537.1603406/94‑1 (MQ=60)

                                      AGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCT                                                        >  SRR4302537.6336741/1‑94 (MQ=60)

                                      AGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCT                                                        >  SRR4302537.3580176/1‑94 (MQ=60)

                                      AGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCT                                                        >  SRR4302537.2886974/1‑94 (MQ=60)

                                       GATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTG                                                       <  SRR4302537.2582867/94‑1 (MQ=60)

                                        ATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGA                                                      >  SRR4302537.116970/1‑94 (MQ=60)

                                         TATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAG                                                     >  SRR4302537.539766/1‑94 (MQ=60)

                                         TATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAG                                                     <  SRR4302537.5363904/94‑1 (MQ=60)

                                         TATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAG                                                     <  SRR4302537.1686220/94‑1 (MQ=60)

                                         TATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAG                                                     >  SRR4302537.2943722/1‑94 (MQ=60)

                                          ATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGG                                                    >  SRR4302537.2200476/1‑94 (MQ=60)

                                          ATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGG                                                    >  SRR4302537.4663063/1‑94 (MQ=60)

                                          ATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGG                                                    <  SRR4302537.695238/94‑1 (MQ=60)

                                           TTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGC                                                   <  SRR4302537.3671459/94‑1 (MQ=60)

                                            TCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCG                                                  <  SRR4302537.1678403/94‑1 (MQ=60)

                                             CCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGG                                                 >  SRR4302537.5572558/1‑94 (MQ=60)

                                             CCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGG                                                 >  SRR4302537.6275672/1‑94 (MQ=60)

                                             CCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGG                                                 >  SRR4302537.5233534/1‑94 (MQ=60)

                                               ATAATGATGCGAGATTCCCCCAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAT                                               <  SRR4302537.5357108/94‑1 (MQ=60)

                                               ATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGAT                                               >  SRR4302537.1424373/1‑94 (MQ=60)

                                                TAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGACCGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATT                                              <  SRR4302537.4583417/94‑1 (MQ=60)

                                                TAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATT                                              <  SRR4302537.5510170/94‑1 (MQ=60)

                                                TAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATT                                              <  SRR4302537.1045006/94‑1 (MQ=60)

                                                TAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATT                                              >  SRR4302537.2245706/1‑94 (MQ=60)

                                                TAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCAGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATT                                              <  SRR4302537.4203969/94‑1 (MQ=60)

                                                 AATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTG                                             <  SRR4302537.5447933/94‑1 (MQ=60)

                                                 AATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTG                                             <  SRR4302537.688674/94‑1 (MQ=60)

                                                  ATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGT                                            >  SRR4302537.4216559/1‑94 (MQ=60)

                                                  ATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGT                                            >  SRR4302537.5202480/1‑94 (MQ=60)

                                                   TGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTC                                           >  SRR4302537.184656/1‑94 (MQ=60)

                                                     ATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCT                                         <  SRR4302537.5155625/94‑1 (MQ=60)

                                                     ATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCT                                         >  SRR4302537.6476503/1‑94 (MQ=60)

                                                      TCCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTT                                        <  SRR4302537.2651815/94‑1 (MQ=60)

                                                         GAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTC                                     >  SRR4302537.4351842/1‑94 (MQ=60)

                                                         GAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTC                                     >  SRR4302537.3571809/1‑94 (MQ=60)

                                                            ATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCAC                                  <  SRR4302537.341251/94‑1 (MQ=60)

                                                            ATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCAC                                  <  SRR4302537.4717337/94‑1 (MQ=60)

                                                            ATTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCAC                                  <  SRR4302537.460989/94‑1 (MQ=60)

                                                             TTCCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACG                                 <  SRR4302537.3630994/94‑1 (MQ=60)

                                                               CCCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTG                               >  SRR4302537.5016997/1‑94 (MQ=60)

                                                                CCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGT                              >  SRR4302537.4669487/1‑94 (MQ=60)

                                                                CCACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGT                              >  SRR4302537.6046828/1‑94 (MQ=60)

                                                                 CACAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTT                             >  SRR4302537.480517/1‑94 (MQ=60)

                                                                   CAGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCG                           <  SRR4302537.4880863/94‑1 (MQ=60)

                                                                    AGGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGA                          >  SRR4302537.3100455/1‑94 (MQ=60)

                                                                     GGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAT                         <  SRR4302537.3404676/94‑1 (MQ=60)

                                                                     GGGTCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGAT                         >  SRR4302537.1699850/1‑94 (MQ=60)

                                                                        TCAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTC                      >  SRR4302537.4091305/1‑94 (MQ=60)

                                                                         CAGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCG                     >  SRR4302537.5840766/1‑94 (MQ=60)

                                                                          AGCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTCGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGA                    >  SRR4302537.1411808/1‑94 (MQ=60)

                                                                           GCTCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAA                   <  SRR4302537.1797082/94‑1 (MQ=60)

                                                                             TCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAAC                 >  SRR4302537.5468911/1‑94 (MQ=60)

                                                                             TCACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAAC                 >  SRR4302537.681683/1‑94 (MQ=60)

                                                                             TCACGATCCACCAGGGGCGGAACGATCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAAC                 <  SRR4302537.5925658/94‑1 (MQ=60)

                                                                              CACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACT                <  SRR4302537.575102/94‑1 (MQ=60)

                                                                              CACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACT                >  SRR4302537.3671904/1‑94 (MQ=60)

                                                                              CACGATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACT                >  SRR4302537.3910290/1‑94 (MQ=60)

                                                                                 GATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTAC             <  SRR4302537.3343658/94‑1 (MQ=60)

                                                                                 GATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTAC             >  SRR4302537.3206405/1‑94 (MQ=60)

                                                                                 GATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTAC             >  SRR4302537.2456034/1‑94 (MQ=60)

                                                                                  ATCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACA            >  SRR4302537.2288050/1‑94 (MQ=60)

                                                                                   TCCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACAC           <  SRR4302537.4061236/94‑1 (MQ=60)

                                                                                    CCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACC          <  SRR4302537.1869240/94‑1 (MQ=60)

                                                                                    CCACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACC          >  SRR4302537.2131153/1‑94 (MQ=60)

                                                                                     CACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCT         <  SRR4302537.5378591/94‑1 (MQ=60)

                                                                                      ACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTT        >  SRR4302537.1040219/1‑94 (MQ=60)

                                                                                      ACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTT        >  SRR4302537.4565220/1‑94 (MQ=60)

                                                                                      ACCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTT        <  SRR4302537.578988/94‑1 (MQ=60)

                                                                                       CCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTT       >  SRR4302537.2864034/1‑94 (MQ=60)

                                                                                       CCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTT       >  SRR4302537.2227484/1‑94 (MQ=60)

                                                                                       CCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTT       >  SRR4302537.3192889/1‑94 (MQ=60)

                                                                                       CCAGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTT       >  SRR4302537.4713513/1‑94 (MQ=60)

                                                                                         AGGGGCTGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCTGATTGTCCTTTCCCACGTGTTCGATCTCGAAACTTACACCTTTCT     <  SRR4302537.6190820/94‑1 (MQ=60)

                                                                                         AGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT     >  SRR4302537.3120178/1‑94 (MQ=60)

                                                                                         AGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT     <  SRR4302537.4125679/94‑1 (MQ=60)

                                                                                         AGGGGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT     >  SRR4302537.3110049/1‑94 (MQ=60)

                                                                                         AGGGGCGGAACGCGCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCT     <  SRR4302537.5479650/94‑1 (MQ=60)

                                                                                            GGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAG  >  SRR4302537.2296987/1‑94 (MQ=60)

                                                                                            GGCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAG  <  SRR4302537.817021/94‑1 (MQ=60)

                                                                                            GGCGGAACGCGCTGAGTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAG  <  SRR4302537.3202468/94‑1 (MQ=60)

AAACAGGCATCAGTGGCGGATGCGGGAAACGCTCATCCAGATATTCCATAATGATGCGAGATTCCCACAGGGTCAGCTCACGATCCACCAGGGTCGGAACGCTCTGATTCGGGTTGAGGTCAATCAGATCCTGAGGCGGATTGTCCTTTTCCACGTGTTCGATCTCGAAACTTACACCTTTCTCAG  >  NC_000913/3374782‑3374967

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 10 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`