Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,395,276	T→C	M59V (ATG→GTG)	cafA ←	CafA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,395,276	0	T	C	100.0%	784.0 / NA	225	M59V (ATG→GTG)	cafA	CafA
Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (119/106); total (119/106)

Only 100 of 235 total aligned reads displayed.

CACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAA  >  NC_000913/3395186‑3395365

cACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCacac                                                                                         >  1:3098657/1‑93 (MQ=255)

 aCCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACAcc                                                                                        >  1:3193897/1‑93 (MQ=255)

   ccGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCg                                                                                      <  2:5995031/93‑1 (MQ=255)

     gCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGa                                                                                    >  2:2336975/1‑93 (MQ=255)

      ccACCCATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGaa                                                                                   <  1:5684319/93‑1 (MQ=255)

       cacacaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAg                                                                                  >  1:5416051/1‑93 (MQ=255)

       cacacaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAg                                                                                  >  2:4122916/1‑93 (MQ=255)

          acaTTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTac                                                                               >  2:1574937/1‑93 (MQ=255)

           caTTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTaca                                                                              <  1:6381139/93‑1 (MQ=255)

           caTTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTaca                                                                              <  1:1228568/93‑1 (MQ=255)

           caTTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTaca                                                                              <  2:2580662/93‑1 (MQ=255)

           caTGCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTaca                                                                              <  2:4852876/93‑1 (MQ=255)

            aTTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTacac                                                                             >  1:1535864/1‑93 (MQ=255)

            aTTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTacac                                                                             >  1:4793616/1‑93 (MQ=255)

               cGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGAc                                                                          >  1:342404/1‑93 (MQ=255)

               cGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGAc                                                                          >  1:2075887/1‑93 (MQ=255)

                ggTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACt                                                                         >  2:4947295/1‑93 (MQ=255)

                 gtgtGCGGCATGATGTCGGATGCATGAAGAACCGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACtt                                                                        <  1:795341/93‑1 (MQ=255)

                 gtgtGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACtt                                                                        >  2:4288958/1‑93 (MQ=255)

                 gtgtGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACtt                                                                        >  2:5539589/1‑93 (MQ=255)

                  tgtgCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACGCCCGGAAGTACACGACTTa                                                                       <  1:172705/93‑1 (MQ=255)

                  tgtgCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTa                                                                       <  1:1587439/93‑1 (MQ=255)

                   gtgCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTac                                                                      <  1:3843141/93‑1 (MQ=255)

                   gtgCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTac                                                                      <  1:5781936/93‑1 (MQ=255)

                   gtgCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTac                                                                      >  1:652492/1‑93 (MQ=255)

                   gtgCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCACAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTac                                                                      >  1:2906401/1‑93 (MQ=255)

                     gCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTacac                                                                    <  2:847250/93‑1 (MQ=255)

                     gCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTacac                                                                    <  1:4643759/93‑1 (MQ=255)

                     gCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTacac                                                                    >  2:3945460/1‑93 (MQ=255)

                      cGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACg                                                                   >  2:1774683/1‑93 (MQ=255)

                       ggCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGa                                                                  >  2:4689549/1‑93 (MQ=255)

                       ggCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGa                                                                  <  2:2141512/93‑1 (MQ=255)

                          atgatgTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGAccc                                                               <  2:3794398/93‑1 (MQ=255)

                          atgatgTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGAccc                                                               >  2:1512481/1‑93 (MQ=255)

                           tgatgTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCt                                                              <  2:5292655/93‑1 (MQ=255)

                           tgatgTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCt                                                              >  2:318036/1‑93 (MQ=255)

                             atgTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGGCCCTTg                                                            <  2:5828338/93‑1 (MQ=255)

                              tgTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGt                                                           >  2:3394641/1‑93 (MQ=255)

                                 cGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGa                                                        >  2:4332774/1‑93 (MQ=255)

                                  ggATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGat                                                       >  2:1358269/1‑93 (MQ=255)

                                  ggATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGat                                                       <  1:3619598/93‑1 (MQ=255)

                                   gATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGata                                                      <  1:3519925/93‑1 (MQ=255)

                                     tGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATAtt                                                    >  1:2732207/1‑93 (MQ=255)

                                     tGCATGAAGAAACGCGGCTTTATCCAGCCCAAGATCTACAAAAGCCGCCTGCACGCCCGGAAGTACACGACTTACACGACCCTTGTAGATAtt                                                    <  1:1632778/93‑1 (MQ=255)

                                     agCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATAtt                                                    <  1:4280193/92‑1 (MQ=255)

                                       cATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGc                                                  <  2:3863179/93‑1 (MQ=255)

                                       cATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGc                                                  >  2:2310551/1‑93 (MQ=255)

                                        aTGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGcc                                                 <  2:3756747/93‑1 (MQ=255)

                                          gaagaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCcta                                               >  1:3588036/1‑93 (MQ=255)

                                          gaagaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCcta                                               >  2:480278/1‑93 (MQ=255)

                                            agaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCctact                                             >  2:1691725/1‑93 (MQ=255)

                                             gaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCTGAAGTACACGACTTACACGACCCTTGTAGATATTGCctacta                                            <  1:107986/93‑1 (MQ=255)

                                             gaaACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCctacta                                            >  2:6133669/1‑93 (MQ=255)

                                             aaaccgcgGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCTGGAAGTACACGACTTACACGACCCTTGTAGATATTGCctacta                                            <  1:39564/89‑1 (MQ=255)

                                              aaaCGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTAt                                           <  1:1857989/93‑1 (MQ=255)

                                               aaCGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTAtt                                          >  1:5601300/1‑93 (MQ=255)

                                                aCGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTc                                         >  1:5016364/1‑93 (MQ=255)

                                                aCGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTc                                         <  2:64042/93‑1 (MQ=255)

                                                     gCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGt                                    <  2:342396/93‑1 (MQ=255)

                                                     gCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGt                                    <  2:1977097/93‑1 (MQ=255)

                                                     gCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGt                                    >  1:5480567/1‑93 (MQ=255)

                                                      cTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTc                                   <  1:1985035/93‑1 (MQ=255)

                                                      cTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTc                                   >  2:2802639/1‑93 (MQ=255)

                                                      cTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTc                                   >  1:5100650/1‑93 (MQ=255)

                                                        ttATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTcgc                                 <  1:3106636/93‑1 (MQ=255)

                                                         tATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTcgcg                                >  2:5727333/1‑93 (MQ=255)

                                                             cAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTc                            <  1:97961/93‑1 (MQ=255)

                                                             cAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTc                            >  2:4329665/1‑93 (MQ=255)

                                                             cAGCCCAATATCTACAAAAGCCGCATGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTc                            <  1:3961683/93‑1 (MQ=255)

                                                             cAGCCCAATAACTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTc                            <  2:5053972/93‑1 (MQ=255)

                                                              aGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCa                           >  1:4347716/1‑93 (MQ=255)

                                                              aGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCa                           >  1:3757631/1‑93 (MQ=255)

                                                              aGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCa                           <  1:3323965/93‑1 (MQ=255)

                                                              aGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCa                           >  1:4220422/1‑93 (MQ=255)

                                                               gCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCAc                          >  2:5178087/1‑93 (MQ=255)

                                                                cccAATATCTACAAAAGCCGCCTGCACCCCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACg                         <  1:4003174/93‑1 (MQ=255)

                                                                 ccAATATCTCCAAACGCCGCCTGCACACCCGGAAGTACACGACTTACACGCCCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGt                        <  1:1343630/93‑1 (MQ=255)

                                                                  cAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGtt                       >  2:1094994/1‑93 (MQ=255)

                                                                   aaTATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTc                      >  1:6056782/1‑93 (MQ=255)

                                                                      atCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAat                   <  2:6210385/93‑1 (MQ=255)

                                                                       tCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAata                  >  2:596690/1‑93 (MQ=255)

                                                                       tCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAata                  <  2:2771888/93‑1 (MQ=255)

                                                                       tCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAata                  >  1:2248914/1‑93 (MQ=255)

                                                                       tCTACAAAAGCCGCCTGCACACACGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAata                  <  1:4892854/93‑1 (MQ=255)

                                                                         tACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATg                <  2:4000430/93‑1 (MQ=255)

                                                                         tACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATg                <  1:6180070/93‑1 (MQ=255)

                                                                         tACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATg                <  1:2643120/93‑1 (MQ=255)

                                                                            aaaaGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAAt             >  2:6054037/1‑93 (MQ=255)

                                                                            aaaaGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAAt             <  1:3669749/93‑1 (MQ=255)

                                                                             aaaGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAAtt            <  1:1969880/93‑1 (MQ=255)

                                                                               acccgccTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTc          <  1:3047102/91‑1 (MQ=255)

                                                                                gccgccTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTcc         <  2:2323986/93‑1 (MQ=255)

                                                                                  cgccTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTg       >  2:671322/1‑93 (MQ=255)

                                                                                  cgccTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCAGCGTCGCGCCTCACGTTCAATATGAATTTCCTg       >  2:1035696/1‑93 (MQ=255)

                                                                                   gccTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGc      <  2:3531903/93‑1 (MQ=255)

                                                                                    ccTGCAGTCCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCa     <  1:1021516/93‑1 (MQ=255)

                                                                                    ccTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCa     >  1:5934139/1‑93 (MQ=255)

                                                                                     cTGCACACCCGGAAGTACACGCCTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAg    >  1:5313885/1‑93 (MQ=255)

                                                                                     cTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAg    >  2:1398944/1‑93 (MQ=255)

                                                                                      tGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGa   >  1:1952029/1‑93 (MQ=255)

CACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAACGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAA  >  NC_000913/3395186‑3395365

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACC  >  NC_000913/3395183‑3395369

CTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCAC                                                                                               >  SRR4302537.3739262/1‑94 (MQ=60)

  TCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACAC                                                                                             >  SRR4302537.3120284/1‑94 (MQ=60)

   CACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACC                                                                                            >  SRR4302537.3216356/1‑94 (MQ=60)

   CACCCGCCACACATTCGGTGTGCGGCATGATGCCGGATGCATGAAGAAA‑CGCGGCATTATCCAGCTCAATATCTACAAAAGCCGCCTGCACACC                                                                                            >  SRR4302537.2063505/1‑94 (MQ=60)

    CCCCGCCACAAATTCGGTGTGCGGCATGATGTCGGATGCAAGAAGCAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCC                                                                                           <  SRR4302537.6512018/94‑1 (MQ=60)

      CCGCCCCACATTCTGTATGCGGCATGATGTCTGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGG                                                                                         <  SRR4302537.3795879/94‑1 (MQ=60)

      CCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGG                                                                                         >  SRR4302537.362423/1‑94 (MQ=60)

       CGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGA                                                                                        >  SRR4302537.982784/1‑94 (MQ=60)

        GCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAA                                                                                       >  SRR4302537.3459782/1‑94 (MQ=60)

         CCACCCATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAG                                                                                      <  SRR4302537.5723196/94‑1 (MQ=60)

         CCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAG                                                                                      <  SRR4302537.3206048/94‑1 (MQ=60)

         CCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAG                                                                                      >  SRR4302537.5452978/1‑94 (MQ=60)

          CACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGT                                                                                     <  SRR4302537.1626703/94‑1 (MQ=60)

            CACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTAC                                                                                   <  SRR4302537.1233290/94‑1 (MQ=60)

            CACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTAC                                                                                   <  SRR4302537.5257220/94‑1 (MQ=60)

             ACATCCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACA                                                                                  <  SRR4302537.2937250/94‑1 (MQ=60)

              CATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACAC                                                                                 >  SRR4302537.4826701/1‑94 (MQ=60)

              CATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACAC                                                                                 <  SRR4302537.1237112/94‑1 (MQ=60)

              CATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACAC                                                                                 <  SRR4302537.5650512/94‑1 (MQ=60)

              CATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACAC                                                                                 <  SRR4302537.6424640/94‑1 (MQ=60)

              CATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACAC                                                                                 >  SRR4302537.1546592/1‑94 (MQ=60)

               ATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACACAAGCCGCCTGCACACCCGGAAGTACACG                                                                                >  SRR4302537.3121481/1‑94 (MQ=60)

               ATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACG                                                                                >  SRR4302537.5169890/1‑94 (MQ=60)

                 TCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGTAAGTACACGAC                                                                              >  SRR4302537.1280400/1‑94 (MQ=60)

                 TCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGAC                                                                              >  SRR4302537.344778/1‑94 (MQ=60)

                 TCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGAC                                                                              >  SRR4302537.2090503/1‑94 (MQ=60)

                    GTGTGCGGCATGATGTCGGATGCATGAAGAAC‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTA                                                                           <  SRR4302537.800796/94‑1 (MQ=60)

                     TGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACGCCCGGAAGTACACGACTTAC                                                                          <  SRR4302537.173933/94‑1 (MQ=60)

                     TGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTAC                                                                          <  SRR4302537.5035661/94‑1 (MQ=60)

                     TGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTAC                                                                          <  SRR4302537.5949800/94‑1 (MQ=60)

                     TGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTAC                                                                          >  SRR4302537.656943/1‑94 (MQ=60)

                     TGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTAC                                                                          <  SRR4302537.1598535/94‑1 (MQ=60)

                     TGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCACAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTAC                                                                          >  SRR4302537.2926818/1‑94 (MQ=60)

                      GTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACA                                                                         <  SRR4302537.3870055/94‑1 (MQ=60)

                      GTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACA                                                                         <  SRR4302537.3555704/94‑1 (MQ=60)

                      GTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACA                                                                         <  SRR4302537.5821567/94‑1 (MQ=60)

                       TGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACAC                                                                        <  SRR4302537.1974281/94‑1 (MQ=60)

                        GCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACG                                                                       <  SRR4302537.4675873/94‑1 (MQ=60)

                        GCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACG                                                                       <  SRR4302537.5061775/94‑1 (MQ=60)

                          GGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGAC                                                                     >  SRR4302537.4714721/1‑94 (MQ=60)

                           GCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACC                                                                    <  SRR4302537.2707319/94‑1 (MQ=60)

                            CATGATGTCGGATGCATGGAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCC                                                                   <  SRR4302537.5777813/94‑1 (MQ=60)

                             ATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCT                                                                  >  SRR4302537.504072/1‑94 (MQ=60)

                              TGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTT                                                                 <  SRR4302537.3290394/94‑1 (MQ=60)

                               GATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTG                                                                >  SRR4302537.6202096/1‑94 (MQ=60)

                                 TGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTA                                                              <  SRR4302537.4079936/94‑1 (MQ=60)

                                  GTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACCCCCGGAAGTACACGACTTACACGACCCTTGTAG                                                             <  SRR4302537.4453236/94‑1 (MQ=60)

                                  GTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAG                                                             <  SRR4302537.3414116/94‑1 (MQ=60)

                                    CGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGAT                                                           >  SRR4302537.2657951/1‑94 (MQ=60)

                                     GGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATA                                                          <  SRR4302537.3645110/94‑1 (MQ=60)

                                      GATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATAT                                                         <  SRR4302537.3544831/94‑1 (MQ=60)

                                       ATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATT                                                        >  SRR4302537.6097022/1‑94 (MQ=60)

                                       ATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATT                                                        <  SRR4302537.5972789/94‑1 (MQ=60)

                                       ATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATT                                                        >  SRR4302537.1990838/1‑94 (MQ=60)

                                       ATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATT                                                        >  SRR4302537.2751279/1‑94 (MQ=60)

                                        TGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAAGATCTACAAAAGCCGCCTGCACGCCCGGAAGTACACGACTTACACGACCCTTGTAGATATTG                                                       <  SRR4302537.1644218/94‑1 (MQ=60)

                                        AGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTG                                                       <  SRR4302537.4309947/94‑1 (MQ=60)

                                          CATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCC                                                     <  SRR4302537.1055685/94‑1 (MQ=60)

                                            TGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTA                                                   >  SRR4302537.3460217/1‑94 (MQ=60)

                                            TGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTA                                                   >  SRR4302537.3666164/1‑94 (MQ=60)

                                            TGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTA                                                   <  SRR4302537.1922407/94‑1 (MQ=60)

                                            TGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTA                                                   >  SRR4302537.3613331/1‑94 (MQ=60)

                                             GAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTAC                                                  >  SRR4302537.413440/1‑94 (MQ=60)

                                              AAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACT                                                 <  SRR4302537.5589983/94‑1 (MQ=60)

                                              AAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACT                                                 <  SRR4302537.774616/94‑1 (MQ=60)

                                               AGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTA                                                >  SRR4302537.4374465/1‑94 (MQ=60)

                                               AGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTA                                                >  SRR4302537.3988714/1‑94 (MQ=60)

                                               AGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGACTACTA                                                >  SRR4302537.4012994/1‑94 (MQ=60)

                                                GAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCTGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTAT                                               <  SRR4302537.108775/94‑1 (MQ=60)

                                                GAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTAT                                               <  SRR4302537.2667846/94‑1 (MQ=60)

                                                 AAACCGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCTGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTAT                                               <  SRR4302537.39864/94‑1 (MQ=60)

                                                 AAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATT                                              <  SRR4302537.282412/94‑1 (MQ=60)

                                                 AAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATT                                              >  SRR4302537.5639591/1‑94 (MQ=60)

                                                 AAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATT                                              <  SRR4302537.1870930/94‑1 (MQ=60)

                                                  AA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTC                                             >  SRR4302537.5050913/1‑94 (MQ=60)

                                                       CGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCG                                         >  SRR4302537.5630956/1‑94 (MQ=60)

                                                        GGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGT                                        >  SRR4302537.4899891/1‑94 (MQ=60)

                                                        GGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGT                                        >  SRR4302537.1504672/1‑94 (MQ=60)

                                                        GGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGT                                        >  SRR4302537.5517983/1‑94 (MQ=60)

                                                         GCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTC                                       >  SRR4302537.5135793/1‑94 (MQ=60)

                                                          CTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCG                                      <  SRR4302537.1998828/94‑1 (MQ=60)

                                                            TTATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCG                                    <  SRR4302537.3128333/94‑1 (MQ=60)

                                                              ATCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCC                                  >  SRR4302537.1958864/1‑94 (MQ=60)

                                                               TCCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCT                                 <  SRR4302537.5452839/94‑1 (MQ=60)

                                                                CCAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCCCCTC                                >  SRR4302537.965149/1‑94 (MQ=60)

                                                                 CAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCA                               <  SRR4302537.98672/94‑1 (MQ=60)

                                                                 CAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCA                               >  SRR4302537.4377848/1‑94 (MQ=60)

                                                                 CAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCA                               >  SRR4302537.4249826/1‑94 (MQ=60)

                                                                 CAGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCA                               >  SRR4302537.3784009/1‑94 (MQ=60)

                                                                 CAGCCCAATATCTACAAAAGCCGCATGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCA                               <  SRR4302537.3989394/94‑1 (MQ=60)

                                                                  AGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCAC                              >  SRR4302537.3146024/1‑94 (MQ=60)

                                                                  AGCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCAC                              <  SRR4302537.3347485/94‑1 (MQ=60)

                                                                   GCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACG                             <  SRR4302537.2038601/94‑1 (MQ=60)

                                                                   GCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACG                             >  SRR4302537.1815870/1‑94 (MQ=60)

                                                                   GCCCAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACG                             <  SRR4302537.1566140/94‑1 (MQ=60)

                                                                    CCCAATATCTACAAAAGCCGCCTGCACCCCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGT                            <  SRR4302537.4031196/94‑1 (MQ=60)

                                                                     CCAATATCTCCAAACGCCGCCTGCACACCCGGAAGTACACGACTTACACGCCCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTT                           <  SRR4302537.1352991/94‑1 (MQ=60)

                                                                      CAATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTC                          >  SRR4302537.6098386/1‑94 (MQ=60)

                                                                       AATATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCA                         >  SRR4302537.1607851/1‑94 (MQ=60)

                                                                         TATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAAT                       <  SRR4302537.379077/94‑1 (MQ=60)

                                                                          ATCTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATA                      >  SRR4302537.2264786/1‑94 (MQ=60)

                                                                           TCTACAAAAGCCGCCTGCACACACGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATAT                     <  SRR4302537.4926638/94‑1 (MQ=60)

                                                                            CTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATG                    >  SRR4302537.6402654/1‑94 (MQ=60)

                                                                            CTACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATG                    <  SRR4302537.426693/94‑1 (MQ=60)

                                                                             TACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGA                   <  SRR4302537.6222639/94‑1 (MQ=60)

                                                                             TACAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGA                   <  SRR4302537.2661611/94‑1 (MQ=60)

                                                                               CAAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAAT                 >  SRR4302537.3946824/1‑94 (MQ=60)

                                                                                AAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATT                <  SRR4302537.3695569/94‑1 (MQ=60)

                                                                                AAAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATT                <  SRR4302537.3105121/94‑1 (MQ=60)

                                                                                 AAAGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTT               <  SRR4302537.1983580/94‑1 (MQ=60)

                                                                                   AGCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCC             <  SRR4302537.1242576/94‑1 (MQ=60)

                                                                                   ACCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCC             <  SRR4302537.3068330/94‑1 (MQ=60)

                                                                                    GCCGCCTGCACCCCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCT            <  SRR4302537.3922394/94‑1 (MQ=60)

                                                                                    GCCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCT            <  SRR4302537.6339306/94‑1 (MQ=60)

                                                                                    ACCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCT            <  SRR4302537.3778853/94‑1 (MQ=60)

                                                                                     CCGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTG           <  SRR4302537.5480575/94‑1 (MQ=60)

                                                                                      CGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGC          >  SRR4302537.4637222/1‑94 (MQ=60)

                                                                                      CGCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGC          <  SRR4302537.3658395/94‑1 (MQ=60)

                                                                                       GCCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCA         >  SRR4302537.5974861/1‑94 (MQ=60)

                                                                                        CCTGCAGTCCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAG        <  SRR4302537.1028554/94‑1 (MQ=60)

                                                                                        CCTGCACACCCGGAAGTACACGCCTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAG        >  SRR4302537.5350380/1‑94 (MQ=60)

                                                                                        CCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAG        >  SRR4302537.3090828/1‑94 (MQ=60)

                                                                                        CCTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAG        <  SRR4302537.1721047/94‑1 (MQ=60)

                                                                                         CTGCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGA       >  SRR4302537.1965610/1‑94 (MQ=60)

                                                                                           GCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGACTTTCCTGCAGAAT     >  SRR4302537.1932569/1‑94 (MQ=60)

                                                                                           GCACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAAT     <  SRR4302537.2130424/94‑1 (MQ=60)

                                                                                            CACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATA    >  SRR4302537.904614/1‑94 (MQ=60)

                                                                                            CACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATA    <  SRR4302537.1154697/94‑1 (MQ=60)

                                                                                             ACACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATAC   <  SRR4302537.5593014/94‑1 (MQ=60)

                                                                                              CACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACC  <  SRR4302537.616207/94‑1 (MQ=60)

                                                                                              CACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACC  <  SRR4302537.5478274/94‑1 (MQ=60)

CTTCACCCGCCACACATTCGGTGTGCGGCATGATGTCGGATGCATGAAGAAA‑CGCGGCTTTATCCAGCCCAATATCTACAAAAGCCGCCTGCATACCCGGAAGTACACGACTTACACGACCCTTGTAGATATTGCCTACTATTCCGCGTCGCGCCTCACGTTCAATATGAATTTCCTGCAGAATACC  >  NC_000913/3395183‑3395369

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`