Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,399,656	A→G	G438G (GGT→GGC)	yhdA ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,399,656	0	A	G	99.6%	953.9 / ‑4.8	271	G438G (GGT→GGC)	yhdA	hypothetical protein
Reads supporting (aligned to +/- strand): ref base A (1/0); new base G (133/137); total (134/137)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.81e-01

Only 100 of 275 total aligned reads displayed.

AAGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCGCG  >  NC_000913/3399566‑3399745

aaGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGcc                                                                                         <  1:4703500/93‑1 (MQ=255)

 aGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCa                                                                                        <  2:5874505/93‑1 (MQ=255)

 aGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCa                                                                                        <  1:6395623/93‑1 (MQ=255)

  gACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCAt                                                                                       <  1:1916700/93‑1 (MQ=255)

   aCGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATc                                                                                      >  2:4576367/1‑93 (MQ=255)

     ggCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGa                                                                                    >  2:1120306/1‑93 (MQ=255)

      gCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGaa                                                                                   >  1:1506759/1‑93 (MQ=255)

      gCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGaa                                                                                   <  2:1255491/93‑1 (MQ=255)

      gCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGaa                                                                                   <  1:1231104/93‑1 (MQ=255)

      gCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGaa                                                                                   >  2:3619399/1‑93 (MQ=255)

      gCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGaa                                                                                   >  2:4330271/1‑93 (MQ=255)

       cTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAg                                                                                  <  1:5211010/93‑1 (MQ=255)

        tGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGa                                                                                 <  1:4740288/93‑1 (MQ=255)

        tGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGa                                                                                 <  2:1588201/93‑1 (MQ=255)

        tGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGa                                                                                 <  2:2943123/93‑1 (MQ=255)

        tGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGa                                                                                 >  2:6426940/1‑93 (MQ=255)

        tGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGa                                                                                 >  2:5520764/1‑93 (MQ=255)

        tGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGa                                                                                 <  2:4697232/93‑1 (MQ=255)

        tGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGa                                                                                 <  2:4729346/93‑1 (MQ=255)

          aTTTGCAGACGGTCATCCTCTTCCGATAAGCCAATCTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATg                                                                               <  2:3695429/93‑1 (MQ=255)

           tttGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGc                                                                              <  1:2080465/93‑1 (MQ=255)

           tttGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGc                                                                              <  1:4728839/93‑1 (MQ=255)

               cAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGca                                                                          <  1:4686753/93‑1 (MQ=255)

                aGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGcac                                                                         >  2:2356802/1‑93 (MQ=255)

                aGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGcac                                                                         >  2:5680602/1‑93 (MQ=255)

                 gACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGcaca                                                                        <  2:714565/93‑1 (MQ=255)

                   cGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATg                                                                      >  1:5281138/1‑93 (MQ=255)

                   cGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATg                                                                      <  1:6195102/93‑1 (MQ=255)

                    tgTCATACTCTTCCGATAAGCCAAACTGCAATACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGa                                                                     <  1:6074658/92‑1 (MQ=255)

                    ggTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGa                                                                     >  1:3338881/1‑93 (MQ=255)

                     gTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAg                                                                    <  2:3233914/93‑1 (MQ=255)

                     gTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAg                                                                    <  1:5610162/93‑1 (MQ=255)

                      tCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGt                                                                   >  1:5715851/1‑93 (MQ=255)

                      tCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGt                                                                   <  1:809048/93‑1 (MQ=255)

                       cATACTCTTCCGATAAGCCAACCTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGtt                                                                  <  2:2717636/93‑1 (MQ=255)

                       cATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGtt                                                                  <  1:2017162/93‑1 (MQ=255)

                        aTACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTc                                                                 >  2:4315182/1‑93 (MQ=255)

                          aCTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTcgc                                                               <  2:1975295/93‑1 (MQ=255)

                           ctctTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTcgcg                                                              >  2:2229677/1‑93 (MQ=255)

                              ttCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGCAGATGCGGCACATGAGTTCGCgatg                                                           >  2:396825/1‑93 (MQ=255)

                                 cGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGatgatg                                                        >  1:413128/1‑93 (MQ=255)

                                 cGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGatgatg                                                        >  2:1004580/1‑93 (MQ=255)

                                  gATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCGCATGAGTTCGCGAtgatga                                                       <  2:6308760/93‑1 (MQ=255)

                                  gATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGAtgatga                                                       <  1:5970822/93‑1 (MQ=255)

                                    tAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAAc                                                     >  2:3566218/1‑93 (MQ=255)

                                     aaGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAAcc                                                    <  1:2971260/93‑1 (MQ=255)

                                     aaGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAAcc                                                    <  2:4629579/93‑1 (MQ=255)

                                      aGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCt                                                   >  1:4379545/1‑93 (MQ=255)

                                      aGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCt                                                   >  2:1455520/1‑93 (MQ=255)

                                      aGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCt                                                   <  2:18655/93‑1 (MQ=255)

                                       gCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTg                                                  >  2:5054280/1‑93 (MQ=255)

                                        ccAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGa                                                 >  1:5128872/1‑93 (MQ=255)

                                         cAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAc                                                >  1:1060078/1‑93 (MQ=255)

                                         cAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAc                                                <  2:2702437/93‑1 (MQ=255)

                                         cAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAc                                                <  2:3854995/93‑1 (MQ=255)

                                         cAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAc                                                >  2:1543307/1‑93 (MQ=255)

                                         cAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAc                                                <  1:2506817/93‑1 (MQ=255)

                                         cAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAc                                                >  1:6133727/1‑93 (MQ=255)

                                           aaCTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCt                                              <  2:3385202/93‑1 (MQ=255)

                                               gCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTcgc                                          >  1:4892680/1‑93 (MQ=255)

                                               gCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTcgc                                          >  2:6180034/1‑93 (MQ=255)

                                                  aGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAg                                       <  1:2718530/93‑1 (MQ=255)

                                                  aGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAg                                       >  2:5400814/1‑93 (MQ=255)

                                                  aGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAg                                       <  1:5460941/93‑1 (MQ=255)

                                                      cATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAc                                   >  2:5169496/1‑93 (MQ=255)

                                                       aTCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAcc                                  <  2:3084927/93‑1 (MQ=255)

                                                       aTCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAcc                                  >  1:707507/1‑93 (MQ=255)

                                                        tCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAccg                                 >  2:4841655/1‑93 (MQ=255)

                                                        tCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAccg                                 >  2:5619375/1‑93 (MQ=255)

                                                         cGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAccgc                                <  1:3090054/93‑1 (MQ=255)

                                                          ggCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAccgcc                               <  1:5883794/93‑1 (MQ=255)

                                                          ggCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAccgcc                               <  2:6459493/93‑1 (MQ=255)

                                                             atatACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGt                            >  1:1651583/1‑93 (MQ=255)

                                                              tataCTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGtt                           <  2:3984561/93‑1 (MQ=255)

                                                               ataCTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGttt                          <  1:3734831/93‑1 (MQ=255)

                                                               ataCTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGttt                          <  1:1618415/93‑1 (MQ=255)

                                                                taCTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGtttt                         >  1:3899383/1‑93 (MQ=255)

                                                                   tCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGa                      >  2:114401/1‑93 (MQ=255)

                                                                       ccgAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATaaa                  <  2:6352112/93‑1 (MQ=255)

                                                                        cgAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAg                 <  1:4520244/93‑1 (MQ=255)

                                                                         gAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAgg                >  2:4100388/1‑93 (MQ=255)

                                                                         gAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAgg                >  1:3449742/1‑93 (MQ=255)

                                                                          aGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGgc               >  2:5239400/1‑93 (MQ=255)

                                                                           gCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGgcg              >  2:1732810/1‑93 (MQ=255)

                                                                            cTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGgcgc             <  1:2658952/93‑1 (MQ=255)

                                                                            cTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGgcgc             <  2:2804505/93‑1 (MQ=255)

                                                                            cTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGgcgc             <  1:1336147/93‑1 (MQ=255)

                                                                              aaCCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCgg           <  1:4475098/93‑1 (MQ=255)

                                                                               aCCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCggg          >  2:6100028/1‑93 (MQ=255)

                                                                                 ctctTCATTGCCATCGAAGATGCGGTACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTGACCGCCGGTTTTTGATAAAGGCGCggggc        >  1:688677/1‑91 (MQ=255)

                                                                                 ctctTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGgcc        >  1:1083947/1‑93 (MQ=255)

                                                                                 ctctTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGgcc        <  2:5818813/93‑1 (MQ=255)

                                                                                     tCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGccgccg    <  1:3645766/93‑1 (MQ=255)

                                                                                     tCATTGCCATCGAAGATGCGGAACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGgccgccc    >  2:2111270/1‑92 (MQ=255)

                                                                                     tCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGccgccg    >  1:3584566/1‑93 (MQ=255)

                                                                                      cATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCcgccgc   <  2:3452601/93‑1 (MQ=255)

                                                                                      cATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCcgccgc   >  1:576377/1‑93 (MQ=255)

                                                                                       aTTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCcgcg  >  2:4207696/1‑93 (MQ=255)

                                                                                       aTTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCcgcg  >  1:2345563/1‑93 (MQ=255)

                                                                                       aTTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCcgcg  >  2:840531/1‑93 (MQ=255)

AAGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCGCG  >  NC_000913/3399566‑3399745

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TAAGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCGCGACT  >  NC_000913/3399565‑3399748

TAAGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCC                                                                                            <  SRR4302537.2152033/94‑1 (MQ=60)

 AAGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCA                                                                                           <  SRR4302537.4736039/94‑1 (MQ=60)

 AAGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCA                                                                                           >  SRR4302537.5662095/1‑94 (MQ=60)

 AAGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCA                                                                                           <  SRR4302537.1570763/94‑1 (MQ=60)

  AGAGGGCTGATTTGCAGACGGTCATACTCTTCCGATAATCCAAGCTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCAT                                                                                          <  SRR4302537.3614930/94‑1 (MQ=60)

  AGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCAT                                                                                          <  SRR4302537.6439178/94‑1 (MQ=60)

   GACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATC                                                                                         <  SRR4302537.1930004/94‑1 (MQ=60)

    ACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCG                                                                                        <  SRR4302537.6248517/94‑1 (MQ=60)

     CGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGA                                                                                       >  SRR4302537.3639887/1‑94 (MQ=60)

     CGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGA                                                                                       >  SRR4302537.4833662/1‑94 (MQ=60)

      GGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAA                                                                                      >  SRR4302537.1517271/1‑94 (MQ=60)

       GCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAG                                                                                     <  SRR4302537.1239665/94‑1 (MQ=60)

        CTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAGGA                                                                                    <  SRR4302537.6294831/94‑1 (MQ=60)

        CTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGA                                                                                    <  SRR4302537.5246842/94‑1 (MQ=60)

         TGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGAT                                                                                   >  SRR4302537.1245720/1‑94 (MQ=60)

         TGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGAT                                                                                   <  SRR4302537.4773052/94‑1 (MQ=60)

           ATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATGCTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGC                                                                                 <  SRR4302537.2768125/94‑1 (MQ=60)

            TTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCG                                                                                <  SRR4302537.2095117/94‑1 (MQ=60)

            TTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCG                                                                                <  SRR4302537.4761522/94‑1 (MQ=60)

              TGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGC                                                                              <  SRR4302537.5504028/94‑1 (MQ=60)

               GCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCA                                                                             <  SRR4302537.208760/94‑1 (MQ=60)

                CAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCAC                                                                            <  SRR4302537.4719178/94‑1 (MQ=60)

                 AGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACA                                                                           >  SRR4302537.4726909/1‑94 (MQ=60)

                   ACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATG                                                                         >  SRR4302537.5317468/1‑94 (MQ=60)

                    CGGTCATCCTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGA                                                                        <  SRR4302537.6173296/94‑1 (MQ=60)

                    CGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGA                                                                        <  SRR4302537.2794267/94‑1 (MQ=60)

                    CGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGA                                                                        >  SRR4302537.3362518/1‑94 (MQ=60)

                    CGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGA                                                                        <  SRR4302537.6237794/94‑1 (MQ=60)

                     TGTCATACTCTTCCGATAAGCCAAACTGCAATACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAG                                                                       <  SRR4302537.6116403/94‑1 (MQ=60)

                      GTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGT                                                                      >  SRR4302537.4067260/1‑94 (MQ=60)

                      GTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGT                                                                      <  SRR4302537.5648517/94‑1 (MQ=60)

                      GTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGT                                                                      >  SRR4302537.5754966/1‑94 (MQ=60)

                      GTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAACATGCGGCACATGAGT                                                                      >  SRR4302537.5984370/1‑94 (MQ=60)

                       TCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTT                                                                     <  SRR4302537.814598/94‑1 (MQ=60)

                       TCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTT                                                                     >  SRR4302537.60067/1‑94 (MQ=60)

                        CATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTC                                                                    <  SRR4302537.2125558/94‑1 (MQ=60)

                        CATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTC                                                                    <  SRR4302537.2031224/94‑1 (MQ=60)

                        CATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTC                                                                    >  SRR4302537.3169098/1‑94 (MQ=60)

                         ATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCG                                                                   >  SRR4302537.4682119/1‑94 (MQ=60)

                          TACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGC                                                                  <  SRR4302537.784149/94‑1 (MQ=60)

                           ACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCG                                                                 <  SRR4302537.250934/94‑1 (MQ=60)

                           ACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCG                                                                 <  SRR4302537.4521326/94‑1 (MQ=60)

                              CTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATG                                                              >  SRR4302537.2228334/1‑94 (MQ=60)

                              CTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATG                                                              <  SRR4302537.4495341/94‑1 (MQ=60)

                              CTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATG                                                              <  SRR4302537.1885857/94‑1 (MQ=60)

                                TCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGAT                                                            >  SRR4302537.1068030/1‑94 (MQ=60)

                                 CCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATG                                                           >  SRR4302537.415987/1‑94 (MQ=60)

                                 CCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATG                                                           <  SRR4302537.4028496/94‑1 (MQ=60)

                                   GATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAA                                                         <  SRR4302537.6011804/94‑1 (MQ=60)

                                   GATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAA                                                         >  SRR4302537.529426/1‑94 (MQ=60)

                                   GATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAA                                                         <  SRR4302537.1218908/94‑1 (MQ=60)

                                    ATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAAC                                                        <  SRR4302537.142479/94‑1 (MQ=60)

                                     TAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGAGGCACATGAGTTCGCGATGATGAACC                                                       >  SRR4302537.5059409/1‑94 (MQ=60)

                                      AAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCT                                                      >  SRR4302537.4409774/1‑94 (MQ=60)

                                      AAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCT                                                      <  SRR4302537.2992083/94‑1 (MQ=60)

                                       AGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTG                                                     <  SRR4302537.3687701/94‑1 (MQ=60)

                                        GCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGA                                                    >  SRR4302537.5164215/1‑94 (MQ=60)

                                         CCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAC                                                   <  SRR4302537.4761960/94‑1 (MQ=60)

                                         CCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAC                                                   >  SRR4302537.6175894/1‑94 (MQ=60)

                                         CCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAC                                                   >  SRR4302537.1067389/1‑94 (MQ=60)

                                         CCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGAC                                                   >  SRR4302537.3260427/1‑94 (MQ=60)

                                          CAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACC                                                  <  SRR4302537.1876137/94‑1 (MQ=60)

                                          CAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACC                                                  <  SRR4302537.2524451/94‑1 (MQ=60)

                                           AAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCT                                                 >  SRR4302537.3843580/1‑94 (MQ=60)

                                           AAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCT                                                 >  SRR4302537.4731099/1‑94 (MQ=60)

                                           AAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCT                                                 >  SRR4302537.3027086/1‑94 (MQ=60)

                                           AAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCT                                                 <  SRR4302537.431483/94‑1 (MQ=60)

                                            AACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTT                                                >  SRR4302537.5760794/1‑94 (MQ=60)

                                             ACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTC                                               <  SRR4302537.3386600/94‑1 (MQ=60)

                                              CTGCAAGACCATCGGCATATACTCCGCCTAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCG                                              <  SRR4302537.3590013/94‑1 (MQ=60)

                                              CTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCG                                              <  SRR4302537.1150485/94‑1 (MQ=60)

                                              CTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCG                                              <  SRR4302537.808816/94‑1 (MQ=60)

                                              CTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCG                                              <  SRR4302537.5845190/94‑1 (MQ=60)

                                               TGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGC                                             >  SRR4302537.5392372/1‑94 (MQ=60)

                                               TGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGC                                             >  SRR4302537.1639957/1‑94 (MQ=60)

                                               TGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGC                                             >  SRR4302537.4926462/1‑94 (MQ=60)

                                                GCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCG                                            <  SRR4302537.1390452/94‑1 (MQ=60)

                                                 CAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGA                                           >  SRR4302537.5899118/1‑94 (MQ=60)

                                                   AGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGT                                         <  SRR4302537.2737513/94‑1 (MQ=60)

                                                   AGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGT                                         <  SRR4302537.5498193/94‑1 (MQ=60)

                                                     ACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAA                                       <  SRR4302537.5224738/94‑1 (MQ=60)

                                                      CCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAAC                                      >  SRR4302537.4954789/1‑94 (MQ=60)

                                                       CATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACC                                     >  SRR4302537.712347/1‑94 (MQ=60)

                                                       CATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACC                                     >  SRR4302537.3082595/1‑94 (MQ=60)

                                                       CATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACC                                     >  SRR4302537.196648/1‑94 (MQ=60)

                                                       CATCGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACC                                     >  SRR4302537.4858655/1‑94 (MQ=60)

                                                          CGGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCC                                  <  SRR4302537.3111606/94‑1 (MQ=60)

                                                           GGCATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCG                                 <  SRR4302537.5924165/94‑1 (MQ=60)

                                                             CATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGT                               >  SRR4302537.1663164/1‑94 (MQ=60)

                                                              ATATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTT                              >  SRR4302537.1300191/1‑94 (MQ=60)

                                                                ATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTT                            <  SRR4302537.1629721/94‑1 (MQ=60)

                                                                ATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTT                            >  SRR4302537.272918/1‑94 (MQ=60)

                                                                ATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTT                            <  SRR4302537.3761064/94‑1 (MQ=60)

                                                                ATACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTT                            >  SRR4302537.3926644/1‑94 (MQ=60)

                                                                  ACTCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTG                          <  SRR4302537.5520114/94‑1 (MQ=60)

                                                                    TCCGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGAT                        >  SRR4302537.5217655/1‑94 (MQ=60)

                                                                      CGCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAA                      <  SRR4302537.1634671/94‑1 (MQ=60)

                                                                       GCCGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAA                     >  SRR4302537.4430850/1‑94 (MQ=60)

                                                                         CGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGG                   >  SRR4302537.459112/1‑94 (MQ=60)

                                                                         CGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGG                   <  SRR4302537.3677871/94‑1 (MQ=60)

                                                                         CGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGG                   >  SRR4302537.3474142/1‑94 (MQ=60)

                                                                         CGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGG                   <  SRR4302537.4551512/94‑1 (MQ=60)

                                                                         CGAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGG                   >  SRR4302537.4741198/1‑94 (MQ=60)

                                                                          GAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGC                  <  SRR4302537.3506172/94‑1 (MQ=60)

                                                                          GAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGC                  <  SRR4302537.944272/94‑1 (MQ=60)

                                                                          GAGCTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGCC                  >  SRR4302537.2451360/1‑94 (MQ=60)

                                                                             CTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCG               <  SRR4302537.1345453/94‑1 (MQ=60)

                                                                             CTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCG               >  SRR4302537.668641/1‑94 (MQ=60)

                                                                             CTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCG               <  SRR4302537.5370175/94‑1 (MQ=60)

                                                                             CTAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCG               <  SRR4302537.2677528/94‑1 (MQ=60)

                                                                              TAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGG              >  SRR4302537.6207631/1‑94 (MQ=60)

                                                                              TAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGG              <  SRR4302537.2531285/94‑1 (MQ=60)

                                                                              TAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGG              >  SRR4302537.5710268/1‑94 (MQ=60)

                                                                              TAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGG              >  SRR4302537.4758062/1‑94 (MQ=60)

                                                                              TAACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGCAGCGG              >  SRR4302537.4277038/1‑94 (MQ=60)

                                                                               AACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGG             <  SRR4302537.4506059/94‑1 (MQ=60)

                                                                               AACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGG             <  SRR4302537.699703/94‑1 (MQ=60)

                                                                                ACCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGC            >  SRR4302537.6225349/1‑94 (MQ=60)

                                                                                 CCTCTTCATTGCCATCGAAGATGCGGTACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTGACCGCCGGTTTTTGATAAAGGCGCGGGGC           >  SRR4302537.693395/1‑94 (MQ=60)

                                                                                 CCTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCC           >  SRR4302537.1091428/1‑94 (MQ=60)

                                                                                  CTCTTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCG          <  SRR4302537.2634399/94‑1 (MQ=60)

                                                                                     TTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCG       <  SRR4302537.6030283/94‑1 (MQ=60)

                                                                                     TTCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCG       <  SRR4302537.6023453/94‑1 (MQ=60)

                                                                                     TTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCG       >  SRR4302537.3609835/1‑94 (MQ=60)

                                                                                      TCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCGC      <  SRR4302537.3671432/94‑1 (MQ=60)

                                                                                      TCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCGC      >  SRR4302537.580325/1‑94 (MQ=60)

                                                                                      TCATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGCGCCGCCGC      >  SRR4302537.6462747/1‑94 (MQ=60)

                                                                                       CATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCGCG     >  SRR4302537.2362077/1‑94 (MQ=60)

                                                                                       CATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCGCG     <  SRR4302537.2018433/94‑1 (MQ=60)

                                                                                        ATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCGCGA    >  SRR4302537.797541/1‑94 (MQ=60)

                                                                                        ATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGGTTTTGATAAAGGCGCGGGGCGCCGCGA    >  SRR4302537.1891249/1‑94 (MQ=60)

                                                                                        ATTGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGCTTTTGATAAAGCCGCGGGCCGCCGCGA    >  SRR4302537.5126356/1‑94 (MQ=60)

                                                                                          TGCCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCGCGACT  <  SRR4302537.3006176/94‑1 (MQ=60)

TAAGACGGCTGATTTGCAGACGGTCATACTCTTCCGATAAGCCAAACTGCAAGACCATCGGCATATACTCCGCCGAGCTAACCTCTTCATTACCATCGAAGATGCGGCACATGAGTTCGCGATGATGAACCTGACCTTCGCGAGTAACCGCCGGTTTTTGATAAAGGCGCGGGCCGCCGCGACT  >  NC_000913/3399565‑3399748

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 13 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`