Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,412,142	T→C	D214D (GAT→GAC)	acrE →	AcrE

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,412,142	0	T	C	98.2%	751.3 / 2.2	221	D214D (GAT→GAC)	acrE	AcrE
Reads supporting (aligned to +/- strand): ref base T (2/2); new base C (98/119); total (100/121)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.52e-01

Only 100 of 229 total aligned reads displayed.

AAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGA  >  NC_000913/3412053‑3412231

aaaTCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGAcgtg                                                                                        <  2:5174976/93‑1 (MQ=255)

aaaTCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGAcgtg                                                                                        <  1:1354389/93‑1 (MQ=255)

 aaTCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGa                                                                                       >  2:822072/1‑93 (MQ=255)

 aaTCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGa                                                                                       >  2:3839821/1‑93 (MQ=255)

     gACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCa                                                                                   <  1:1179393/93‑1 (MQ=255)

     gACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCa                                                                                   >  1:6406902/1‑93 (MQ=255)

      aCTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCaa                                                                                  <  1:856716/93‑1 (MQ=255)

        tgtgACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATc                                                                                >  2:6365276/1‑93 (MQ=255)

            aCCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGc                                                                            >  2:6475324/1‑93 (MQ=255)

             ccGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCa                                                                           >  2:2310391/1‑93 (MQ=255)

               gAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAAc                                                                         >  1:1980574/1‑93 (MQ=255)

               gAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAAc                                                                         >  2:2219049/1‑93 (MQ=255)

               gAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAAc                                                                         <  1:5485728/93‑1 (MQ=255)

                aaGGCGCTCTTGTCACTAATGGGCGAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACg                                                                        >  2:6230131/1‑93 (MQ=255)

                 aGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGa                                                                       <  2:5350658/93‑1 (MQ=255)

                  ggCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGAt                                                                      >  2:6107025/1‑93 (MQ=255)

                  ggCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGAt                                                                      >  2:1928310/1‑93 (MQ=255)

                  ggCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGAt                                                                      >  2:2579965/1‑93 (MQ=255)

                    cgcTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCATCGAttt                                                                    <  1:4269380/93‑1 (MQ=255)

                    cgcTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGAttt                                                                    <  1:2689393/93‑1 (MQ=255)

                      ctctTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTa                                                                  >  2:1004165/1‑93 (MQ=255)

                      ctctTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTa                                                                  <  2:2789900/93‑1 (MQ=255)

                       tctTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTAt                                                                 <  2:2449769/93‑1 (MQ=255)

                       tctTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTAt                                                                 >  2:116066/1‑93 (MQ=255)

                        tttGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGTAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATg                                                                <  2:2227886/92‑1 (MQ=255)

                         ttGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGa                                                               >  2:4493250/1‑93 (MQ=255)

                           gTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAgg                                                             >  2:4793897/1‑93 (MQ=255)

                           gTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAgg                                                             <  1:4289077/93‑1 (MQ=255)

                           gTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAgg                                                             <  2:3379061/93‑1 (MQ=255)

                            tCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGc                                                            >  1:3490477/1‑93 (MQ=255)

                            tCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGc                                                            <  1:629393/93‑1 (MQ=255)

                            tCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGc                                                            >  1:2422909/1‑93 (MQ=255)

                             cACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCt                                                           >  2:2093752/1‑93 (MQ=255)

                              aCTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTg                                                          <  2:767490/93‑1 (MQ=255)

                               cTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGa                                                         <  2:1673650/93‑1 (MQ=255)

                               cTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGa                                                         <  2:5739950/93‑1 (MQ=255)

                                tAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGaa                                                        <  1:5390037/93‑1 (MQ=255)

                                 aaTGGGCAAACGACTGAACTGGCGACTGTCCAGTAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAg                                                       <  1:6018744/93‑1 (MQ=255)

                                 aaTGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAg                                                       <  1:5026673/93‑1 (MQ=255)

                                  aTGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGc                                                      <  1:4052655/93‑1 (MQ=255)

                                  aTGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGc                                                      >  1:2248872/1‑93 (MQ=255)

                                    gggCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCaa                                                    <  1:2956556/93‑1 (MQ=255)

                                     ggCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAAt                                                   <  1:2732998/93‑1 (MQ=255)

                                      gCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATc                                                  <  2:2971827/93‑1 (MQ=255)

                                         aaCGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGt                                               <  1:636065/93‑1 (MQ=255)

                                            taCTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTaga                                            <  2:2320672/92‑1 (MQ=255)

                                             aCTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTagag                                           >  2:1603646/1‑93 (MQ=255)

                                             aCTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTagag                                           >  2:3541464/1‑93 (MQ=255)

                                              cTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGc                                          <  1:1627118/93‑1 (MQ=255)

                                               tGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCa                                         <  1:2667612/93‑1 (MQ=255)

                                                gAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCaa                                        <  1:2881279/93‑1 (MQ=255)

                                                  aCTGGCGAGTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAgg                                      <  1:3561714/93‑1 (MQ=255)

                                                  aCTGGCGACTGCCCGCCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTCTTTGAGGCTGAAGCAATCCGTAGAGCaacg                                      >  2:842034/1‑91 (MQ=255)

                                                    tGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGaa                                    >  2:1971758/1‑93 (MQ=255)

                                                     ggCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGaaa                                   >  1:1805255/1‑93 (MQ=255)

                                                     ggCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGaaa                                   >  2:5466211/1‑93 (MQ=255)

                                                     ggCGACTGTCCAGCAGCTCGATCCTATCTACGTCGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGaaa                                   <  1:3086103/93‑1 (MQ=255)

                                                      gCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAAt                                  <  1:2132651/93‑1 (MQ=255)

                                                       cGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAAtt                                 >  1:5131378/1‑93 (MQ=255)

                                                       cGACTGTCCAGCAGCTCGATCCTATCTACGTTGACCTGACCCAATCCAGCAACGATTTTATGAGGCTAAACCAATCCGTAGAGCAAGGAAAtt                                 <  2:3198075/93‑1 (MQ=255)

                                                        gACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAAttt                                <  1:5771465/93‑1 (MQ=255)

                                                         aCTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTg                               >  2:4989270/1‑93 (MQ=255)

                                                         aCTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTg                               >  1:5688415/1‑93 (MQ=255)

                                                          cTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGc                              <  2:2179715/93‑1 (MQ=255)

                                                           tGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCa                             >  2:4360917/1‑93 (MQ=255)

                                                           tGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCa                             >  2:310141/1‑93 (MQ=255)

                                                            gTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCAt                            >  1:1680796/1‑93 (MQ=255)

                                                             tCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATa                           <  2:1137886/93‑1 (MQ=255)

                                                               cagcagCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAg                         <  1:89996/93‑1 (MQ=255)

                                                                agcagcTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAgg                        <  1:233552/93‑1 (MQ=255)

                                                                agcagcTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAgg                        >  1:1219604/1‑93 (MQ=255)

                                                                 gcagcTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGa                       <  1:1015932/93‑1 (MQ=255)

                                                                  cagcTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGaa                      <  2:626681/93‑1 (MQ=255)

                                                                  cagcTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGaa                      >  2:5834836/1‑93 (MQ=255)

                                                                  cagcTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGaa                      <  1:4431057/93‑1 (MQ=255)

                                                                   agcTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGaaa                     >  2:2427839/1‑93 (MQ=255)

                                                                     cTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAAc                   <  2:3800171/93‑1 (MQ=255)

                                                                     cTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAAc                   <  1:3286178/93‑1 (MQ=255)

                                                                      tCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACg                  >  1:6263623/1‑93 (MQ=255)

                                                                       cGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGc                 <  1:2559291/93‑1 (MQ=255)

                                                                        gATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGcc                >  2:5053729/1‑93 (MQ=255)

                                                                        gATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGcc                <  1:3949194/93‑1 (MQ=255)

                                                                        gATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGcc                >  2:2021506/1‑93 (MQ=255)

                                                                         aTCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGcca               >  2:1775916/1‑93 (MQ=255)

                                                                         aTCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGcca               >  1:2304516/1‑93 (MQ=255)

                                                                         aTCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGcca               >  1:2761860/1‑93 (MQ=255)

                                                                          tCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGccac              >  2:266620/1‑93 (MQ=255)

                                                                          tCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGccac              >  2:1738366/1‑93 (MQ=255)

                                                                          tCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGccac              >  2:5282771/1‑93 (MQ=255)

                                                                            cTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGccacca            <  2:2677790/93‑1 (MQ=255)

                                                                              aTCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGc          >  2:4084605/1‑93 (MQ=255)

                                                                               tCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCa         <  1:5613665/93‑1 (MQ=255)

                                                                                cTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCaa        <  1:4857197/93‑1 (MQ=255)

                                                                                cTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCaa        <  1:4473871/93‑1 (MQ=255)

                                                                                 tACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAAc       >  1:768754/1‑93 (MQ=255)

                                                                                   cGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGt     <  1:335752/93‑1 (MQ=255)

                                                                                   cGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGt     <  1:4928464/93‑1 (MQ=255)

                                                                                   cGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGt     >  2:2128892/1‑93 (MQ=255)

                                                                                   cGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGt     <  2:4129276/93‑1 (MQ=255)

                                                                                    gTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTa    >  1:3733836/1‑93 (MQ=255)

AAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGA  >  NC_000913/3412053‑3412231

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGT  >  NC_000913/3412049‑3412233

CGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGAC                                                                                             >  SRR4302537.2655514/1‑94 (MQ=60)

CGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGAC                                                                                             >  SRR4302537.3885615/1‑94 (MQ=60)

 GGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACG                                                                                            >  SRR4302537.1242659/1‑94 (MQ=60)

 GGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACG                                                                                            <  SRR4302537.2832296/94‑1 (MQ=60)

 GGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACG                                                                                            >  SRR4302537.1861920/1‑94 (MQ=60)

    AAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGA                                                                                         <  SRR4302537.1363827/94‑1 (MQ=60)

     AATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGAC                                                                                        >  SRR4302537.5141165/1‑94 (MQ=60)

       TCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCC                                                                                      <  SRR4302537.2169843/94‑1 (MQ=60)

        CGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCA                                                                                     >  SRR4302537.6450505/1‑94 (MQ=60)

         GACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAA                                                                                    <  SRR4302537.1187602/94‑1 (MQ=60)

          ACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAAT                                                                                   <  SRR4302537.862596/94‑1 (MQ=60)

          ACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAAT                                                                                   <  SRR4302537.4499188/94‑1 (MQ=60)

            TGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCC                                                                                 <  SRR4302537.97136/94‑1 (MQ=60)

             GTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCA                                                                                <  SRR4302537.1966922/94‑1 (MQ=60)

              TGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAG                                                                               >  SRR4302537.6385695/1‑94 (MQ=60)

               GACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGC                                                                              >  SRR4302537.5532822/1‑94 (MQ=60)

               GACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGC                                                                              <  SRR4302537.2886831/94‑1 (MQ=60)

                  CGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAAC                                                                           >  SRR4302537.1994334/1‑94 (MQ=60)

                   GAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACG                                                                          >  SRR4302537.2364386/1‑94 (MQ=60)

                   GAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACG                                                                          >  SRR4302537.4565277/1‑94 (MQ=60)

                   GAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACG                                                                          <  SRR4302537.5523176/94‑1 (MQ=60)

                    AAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTCTCTACGTTGACATGACCCAATCCAGCAACGA                                                                         >  SRR4302537.643097/1‑94 (MQ=60)

                     AGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGAT                                                                        <  SRR4302537.5162750/94‑1 (MQ=60)

                       GCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTT                                                                      >  SRR4302537.4965607/1‑94 (MQ=60)

                        CGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCATCGATTTT                                                                     <  SRR4302537.4299044/94‑1 (MQ=60)

                        CGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTT                                                                     <  SRR4302537.2708162/94‑1 (MQ=60)

                        CGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTT                                                                     >  SRR4302537.3674522/1‑94 (MQ=60)

                         GCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTA                                                                    >  SRR4302537.2333794/1‑94 (MQ=60)

                          CTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTAT                                                                   <  SRR4302537.807356/94‑1 (MQ=60)

                          CTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTAT                                                                   <  SRR4302537.2107945/94‑1 (MQ=60)

                           TCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATG                                                                  >  SRR4302537.1705859/1‑94 (MQ=60)

                              TGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGG                                                               >  SRR4302537.1108694/1‑94 (MQ=60)

                               GTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGC                                                              >  SRR4302537.1680411/1‑94 (MQ=60)

                               GTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGC                                                              <  SRR4302537.4318891/94‑1 (MQ=60)

                               GTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGC                                                              >  SRR4302537.3515169/1‑94 (MQ=60)

                               GTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGC                                                              >  SRR4302537.2439977/1‑94 (MQ=60)

                               GTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTACGAGGT                                                              >  SRR4302537.5489881/1‑94 (MQ=60)

                                TCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCT                                                             <  SRR4302537.633675/94‑1 (MQ=60)

                                   CTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAA                                                          <  SRR4302537.2324325/94‑1 (MQ=60)

                                   CTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAA                                                          >  SRR4302537.2932125/1‑94 (MQ=60)

                                    TAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAG                                                         <  SRR4302537.5426862/94‑1 (MQ=60)

                                     AATGGGCAAACGACTGAACTGGCGACTGTCCAGTAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGC                                                        <  SRR4302537.6060092/94‑1 (MQ=60)

                                     AATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGC                                                        <  SRR4302537.360737/94‑1 (MQ=60)

                                     AATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGC                                                        >  SRR4302537.2264744/1‑94 (MQ=60)

                                     AATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGC                                                        <  SRR4302537.5061288/94‑1 (MQ=60)

                                      ATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCA                                                       <  SRR4302537.4080993/94‑1 (MQ=60)

                                      ATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCA                                                       <  SRR4302537.453777/94‑1 (MQ=60)

                                        GGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAAT                                                     <  SRR4302537.270017/94‑1 (MQ=60)

                                        GGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAAT                                                     <  SRR4302537.2977299/94‑1 (MQ=60)

                                         GGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATC                                                    <  SRR4302537.2752075/94‑1 (MQ=60)

                                         GGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATC                                                    <  SRR4302537.1514682/94‑1 (MQ=60)

                                          GCAAACGACTGAACTGGCGACTGTCCAGCAGCTCTATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCC                                                   <  SRR4302537.4156021/94‑1 (MQ=60)

                                           CAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGANTTTATGAGGCTGANGNANTNCG                                                  >  SRR4302537.6027870/1‑94 (MQ=60)

                                           AAAACGACTGAACTGGCGTCTGTCCAGCAGCACGATCCTATGTAGGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCG                                                  <  SRR4302537.2502887/94‑1 (MQ=60)

                                            AAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGT                                                 >  SRR4302537.668200/1‑94 (MQ=60)

                                            AAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGT                                                 >  SRR4302537.2747451/1‑94 (MQ=60)

                                             AACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTA                                                <  SRR4302537.640391/94‑1 (MQ=60)

                                              ACGACTGAACTGGCGACTGTCCAGCAGCTAGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAG                                               <  SRR4302537.600210/94‑1 (MQ=60)

                                                GACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAG                                             >  SRR4302537.1212090/1‑94 (MQ=60)

                                                 ACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGC                                            <  SRR4302537.3426569/94‑1 (MQ=60)

                                                 ACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGC                                            <  SRR4302537.5626324/94‑1 (MQ=60)

                                                  CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCA                                           <  SRR4302537.4304680/94‑1 (MQ=60)

                                                  CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCA                                           <  SRR4302537.1638494/94‑1 (MQ=60)

                                                  CTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCA                                           >  SRR4302537.3947927/1‑94 (MQ=60)

                                                   TGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAA                                          <  SRR4302537.2686249/94‑1 (MQ=60)

                                                    GAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAG                                         <  SRR4302537.945933/94‑1 (MQ=60)

                                                    GAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAG                                         <  SRR4302537.2901502/94‑1 (MQ=60)

                                                      ACTGGCGAGTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGA                                       <  SRR4302537.3586838/94‑1 (MQ=60)

                                                       CTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAA                                      >  SRR4302537.2880120/1‑94 (MQ=60)

                                                        TGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAA                                     >  SRR4302537.1817851/1‑94 (MQ=60)

                                                         GGCGACTGTCCAGCAGCTCGATCCTATCTACGTCGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAAT                                    <  SRR4302537.3107607/94‑1 (MQ=60)

                                                          GCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATT                                   <  SRR4302537.2147662/94‑1 (MQ=60)

                                                          GCGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATT                                   >  SRR4302537.5166739/1‑94 (MQ=60)

                                                           CGACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTT                                  <  SRR4302537.4892360/94‑1 (MQ=60)

                                                            GACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTG                                 >  SRR4302537.5727325/1‑94 (MQ=60)

                                                            GACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTG                                 <  SRR4302537.5811024/94‑1 (MQ=60)

                                                            GACTGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTG                                 >  SRR4302537.1267306/1‑94 (MQ=60)

                                                               TGTCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCAT                              >  SRR4302537.1692563/1‑94 (MQ=60)

                                                                 TCCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAA                            <  SRR4302537.86568/94‑1 (MQ=60)

                                                                  CCAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAG                           >  SRR4302537.3035529/1‑94 (MQ=60)

                                                                   CAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGG                          <  SRR4302537.90655/94‑1 (MQ=60)

                                                                   CAGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGG                          >  SRR4302537.1228088/1‑94 (MQ=60)

                                                                    AGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGA                         <  SRR4302537.235201/94‑1 (MQ=60)

                                                                    AGCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGA                         <  SRR4302537.3109034/94‑1 (MQ=60)

                                                                     GCAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAA                        <  SRR4302537.1022928/94‑1 (MQ=60)

                                                                      CAGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAA                       <  SRR4302537.4461656/94‑1 (MQ=60)

                                                                       AGCTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAA                      <  SRR4302537.3565544/94‑1 (MQ=60)

                                                                         CTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACG                    >  SRR4302537.6306620/1‑94 (MQ=60)

                                                                         CTCGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACG                    <  SRR4302537.3309395/94‑1 (MQ=60)

                                                                           CGATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCC                  <  SRR4302537.2577227/94‑1 (MQ=60)

                                                                            GATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCA                 >  SRR4302537.2320780/1‑94 (MQ=60)

                                                                            GATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCA                 >  SRR4302537.2781128/1‑94 (MQ=60)

                                                                            GATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCA                 <  SRR4302537.3976816/94‑1 (MQ=60)

                                                                             ATCCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCAC                >  SRR4302537.1378123/1‑94 (MQ=60)

                                                                               TCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCA              <  SRR4302537.2774936/94‑1 (MQ=60)

                                                                               CCTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCA              <  SRR4302537.2582990/94‑1 (MQ=60)

                                                                                CTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAG             <  SRR4302537.2671967/94‑1 (MQ=60)

                                                                                CTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAACACGCCACCAG             >  SRR4302537.5208488/1‑94 (MQ=60)

                                                                                CTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAG             <  SRR4302537.6408691/94‑1 (MQ=60)

                                                                                CTATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAG             <  SRR4302537.6186613/94‑1 (MQ=60)

                                                                                 TATCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGC            >  SRR4302537.2307870/1‑94 (MQ=60)

                                                                                   TCTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAA          <  SRR4302537.5652047/94‑1 (MQ=60)

                                                                                    CTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAAC         >  SRR4302537.774025/1‑94 (MQ=60)

                                                                                    CTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAAC         <  SRR4302537.3375606/94‑1 (MQ=60)

                                                                                    CTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAAC         <  SRR4302537.4890711/94‑1 (MQ=60)

                                                                                    CTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAAC         >  SRR4302537.5808815/1‑94 (MQ=60)

                                                                                    CTACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAAC         <  SRR4302537.4504820/94‑1 (MQ=60)

                                                                                     TACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACG        <  SRR4302537.4391758/94‑1 (MQ=60)

                                                                                      ACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGT       <  SRR4302537.571713/94‑1 (MQ=60)

                                                                                      ACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGT       <  SRR4302537.416182/94‑1 (MQ=60)

                                                                                      ACGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGT       <  SRR4302537.1835480/94‑1 (MQ=60)

                                                                                       CGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTA      <  SRR4302537.338075/94‑1 (MQ=60)

                                                                                       CGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTA      >  SRR4302537.3760064/1‑94 (MQ=60)

                                                                                       CGTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTA      <  SRR4302537.4962437/94‑1 (MQ=60)

                                                                                        GTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAG     >  SRR4302537.6222266/1‑94 (MQ=60)

                                                                                        GTTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAG     >  SRR4302537.6325396/1‑94 (MQ=60)

                                                                                         TTGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGA    >  SRR4302537.3226840/1‑94 (MQ=60)

                                                                                          TGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAG   >  SRR4302537.3922784/1‑94 (MQ=60)

                                                                                          TGACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAG   <  SRR4302537.4035123/94‑1 (MQ=60)

                                                                                           GACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGT  <  SRR4302537.6067910/94‑1 (MQ=60)

                                                                                           GACGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGT  <  SRR4302537.5672407/94‑1 (MQ=60)

CGGCAAATCGACTGTGACCGAAGGCGCTCTTGTCACTAATGGGCAAACGACTGAACTGGCGACTGTCCAGCAGCTCGATCCTATCTACGTTGATGTGACCCAATCCAGCAACGATTTTATGAGGCTGAAGCAATCCGTAGAGCAAGGAAATTTGCATAAGGAAAACGCCACCAGCAACGTAGAGT  >  NC_000913/3412049‑3412233

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`