Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,418,840	+G	coding (1070/1089 nt)	b3269 →	putative transport system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,418,837	1	.	G	98.0%	683.2 / 4.4	201	coding (1067/1089 nt)	b3269	putative transport system permease protein
Reads supporting (aligned to +/- strand): ref base . (0/4); new base G (92/105); total (92/109)
Fisher's exact test for biased strand distribution p-value = 1.27e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 229 total aligned reads displayed.

GTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC  >  NC_000913/3418745‑3418924

gTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAc                                                                                            <  1:4688144/93‑1 (MQ=255)

gTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAc                                                                                            >  2:5641544/1‑93 (MQ=255)

 tATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑g                                                                                          <  1:1780050/93‑1 (MQ=255)

 tATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑g                                                                                          >  1:86065/1‑93 (MQ=255)

 tATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑g                                                                                          >  1:6289511/1‑93 (MQ=255)

 tATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑g                                                                                          <  1:613459/93‑1 (MQ=255)

  aTCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑gg                                                                                         <  1:5574173/93‑1 (MQ=255)

    ctcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑gg‑gg                                                                                      <  1:6024604/93‑2 (MQ=255)

     tcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑gg‑gga                                                                                     >  2:1105539/1‑91 (MQ=255)

       aaCATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑gg‑ggcaa                                                                                   >  1:2818650/1‑89 (MQ=255)

       aaCATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑gg‑ggcaa                                                                                   >  2:5725972/1‑89 (MQ=255)

         cATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGc                                                                                 <  1:1342105/93‑1 (MQ=255)

          aTCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGcc                                                                                <  1:1604626/93‑1 (MQ=255)

            cGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCAt                                                                              >  2:603572/1‑93 (MQ=255)

            cGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCAt                                                                              >  1:5705129/1‑93 (MQ=255)

             gTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATc                                                                             >  1:2677576/1‑93 (MQ=255)

                aaaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCgag                                                                          <  1:2044496/93‑1 (MQ=255)

                aaaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCgag                                                                          >  2:5974338/1‑93 (MQ=255)

                 aaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCgaga                                                                         >  1:2217550/1‑93 (MQ=255)

                 aaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCgaga                                                                         >  1:5674635/1‑93 (MQ=255)

                     ctcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGAt                                                                     >  1:1989874/1‑93 (MQ=255)

                      tcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATa                                                                    <  1:3785747/93‑1 (MQ=255)

                      tcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATa                                                                    >  2:738586/1‑93 (MQ=255)

                      tcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATa                                                                    <  1:2781802/93‑1 (MQ=255)

                      tcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTATTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATa                                                                    <  1:6081785/93‑1 (MQ=255)

                       cctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATaa                                                                   <  2:6208806/93‑2 (MQ=255)

                       cctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAg                                                                   <  2:3919889/93‑1 (MQ=255)

                       cctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAg                                                                   >  2:3294693/1‑93 (MQ=255)

                       cctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAg                                                                   >  1:4423961/1‑93 (MQ=255)

                       cctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAg                                                                   <  2:5144374/93‑1 (MQ=255)

                         tctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGcc                                                                 >  1:2319912/1‑93 (MQ=255)

                         tctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGcc                                                                 >  1:4867056/1‑93 (MQ=255)

                         tctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGcc                                                                 >  1:950421/1‑93 (MQ=255)

                         tctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGcc                                                                 >  2:5354832/1‑93 (MQ=255)

                         tctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGcc                                                                 >  1:6177616/1‑93 (MQ=255)

                          ctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCa                                                                <  2:4350881/93‑1 (MQ=255)

                          ctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCa                                                                <  1:319281/93‑1 (MQ=255)

                          ctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCa                                                                <  2:2204305/93‑1 (MQ=255)

                            ctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATg                                                              <  2:3709332/93‑1 (MQ=255)

                             ttGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGa                                                             <  2:4614169/93‑1 (MQ=255)

                              tGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGAc                                                            <  2:5781049/93‑1 (MQ=255)

                              tGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGAc                                                            >  1:4432519/1‑93 (MQ=255)

                              tGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGAc                                                            >  2:6014984/1‑93 (MQ=255)

                                ccgccgCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCa                                                          <  1:5260780/93‑1 (MQ=255)

                                  gccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATg                                                        >  1:5193714/1‑93 (MQ=255)

                                   ccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGt                                                       >  1:6224224/1‑93 (MQ=255)

                                   ccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGt                                                       <  1:1841564/93‑1 (MQ=255)

                                     gcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCg                                                     >  2:4994712/1‑93 (MQ=255)

                                      cTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCgg                                                    <  1:461183/93‑1 (MQ=255)

                                      cTATGGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCgg                                                    <  2:3987666/93‑1 (MQ=255)

                                      cTATGGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCgg                                                    <  1:652559/93‑1 (MQ=255)

                                       tATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCATACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGt                                                   >  1:3706741/1‑93 (MQ=255)

                                        aTTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTc                                                  <  1:1891291/93‑1 (MQ=255)

                                          tGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTa                                                <  2:2694374/93‑1 (MQ=255)

                                              tATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTg                                            >  1:1109282/1‑93 (MQ=255)

                                               aTCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGa                                           >  1:3585541/1‑93 (MQ=255)

                                                tCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAt                                          <  1:3950890/93‑1 (MQ=255)

                                                tCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAt                                          >  1:2608563/1‑93 (MQ=255)

                                                  ccGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTa                                        >  1:4775897/1‑93 (MQ=255)

                                                  ccGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTa                                        <  1:2487850/93‑1 (MQ=255)

                                                  ccGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTa                                        <  1:5178229/93‑1 (MQ=255)

                                                   cGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTAt                                       >  1:5966269/1‑93 (MQ=255)

                                                    gATATGGTTTCGCTGTTTGCCGGCCCCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATc                                      <  2:1754974/93‑1 (MQ=255)

                                                    gATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATc                                      <  1:131805/93‑1 (MQ=255)

                                                    gATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATc                                      >  1:2947472/1‑93 (MQ=255)

                                                    gATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATc                                      <  1:830930/93‑1 (MQ=255)

                                                      tatGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGCCGATAGCCATGACCATGTCGGTCTATCTGATTATCAg                                    >  1:1436074/1‑93 (MQ=255)

                                                      tatGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAg                                    >  2:1292291/1‑93 (MQ=255)

                                                       atGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGc                                   >  2:2466846/1‑93 (MQ=255)

                                                       atGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGc                                   >  1:3314367/1‑93 (MQ=255)

                                                        tGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGcc                                  <  1:3433297/93‑1 (MQ=255)

                                                        tGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGcc                                  >  1:5482796/1‑93 (MQ=255)

                                                        tGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGTG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGcc                                  <  1:1262993/93‑1 (MQ=255)

                                                         ggTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCt                                 >  2:2949782/1‑93 (MQ=255)

                                                         ggTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCt                                 >  1:6418269/1‑93 (MQ=255)

                                                            ttCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGAc                              <  1:2182556/93‑1 (MQ=255)

                                                            tgcGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGAc                              <  1:3743730/91‑1 (MQ=255)

                                                               gCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAt                           >  1:3239929/1‑93 (MQ=255)

                                                               gCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAt                           <  1:2162140/93‑1 (MQ=255)

                                                                 tGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         >  2:4860523/1‑93 (MQ=255)

                                                                 tGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         <  1:2347468/93‑1 (MQ=255)

                                                                  ttttGCCGGCCCCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGGCCATGTCGGTCTATCTGATTATCAGCCTGACTAtctc                        <  2:4343371/92‑1 (MQ=255)

                                                                  gTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtctc                        >  1:6428693/1‑93 (MQ=255)

                                                                  gTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtctc                        <  1:2591583/93‑1 (MQ=255)

                                                                    ttGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgc                      <  2:2884868/93‑1 (MQ=255)

                                                                     tGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgct                     <  2:473245/93‑1 (MQ=255)

                                                                      gCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgctg                    >  2:443389/1‑93 (MQ=255)

                                                                       ccGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCTGCCTGACTATCTCgctgc                   >  2:5477403/1‑93 (MQ=255)

                                                                        cGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgctgct                  <  1:3666081/93‑1 (MQ=255)

                                                                        cGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgctgct                  <  2:6371762/93‑1 (MQ=255)

                                                                         ggCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGctgctg                 >  2:2761137/1‑93 (MQ=255)

                                                                          gCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtga                >  2:1673208/1‑93 (MQ=255)

                                                                          gCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtga                <  2:2380351/93‑1 (MQ=255)

                                                                          gCACCGTGCGCAATCAGAC‑GG‑GCCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtga                <  2:4482182/93‑1 (MQ=255)

                                                                            aCCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgatg              <  1:4305277/93‑1 (MQ=255)

                                                                            aCCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgatg              >  2:5394469/1‑93 (MQ=255)

                                                                               gTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAat           >  2:31914/1‑93 (MQ=255)

                                                                               gTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGAATATCTCGCTGCTGATGaaa           >  2:5922309/1‑92 (MQ=255)

                                                                                     aaTCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtat     <  1:1491306/93‑1 (MQ=255)

                                                                                      aTCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCtata    <  1:289007/93‑1 (MQ=255)

GTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC  >  NC_000913/3418745‑3418924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATC  >  NC_000913/3418747‑3418933

ATCTCACCATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GG‑G                                                                                                <  SRR4302537.2720110/94‑1 (MQ=60)

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GG‑G                                                                                                >  SRR4302537.3372721/1‑94 (MQ=60)

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GG‑G                                                                                                <  SRR4302537.5612272/94‑1 (MQ=60)

 TCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GG‑G‑G                                                                                              <  SRR4302537.6103867/94‑1 (MQ=60)

  CTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GG‑Ggc                                                                                              <  SRR4302537.6066002/94‑3 (MQ=60)

   TCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GG‑Ggca                                                                                             <  SRR4302537.3854414/94‑4 (MQ=60)

    CAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAA                                                                                            <  SRR4302537.2451931/94‑1 (MQ=60)

    CAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAA                                                                                            >  SRR4302537.2838357/1‑94 (MQ=60)

     AACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAG                                                                                           >  SRR4302537.6234645/1‑94 (MQ=60)

      ACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGC                                                                                          >  SRR4302537.2689079/1‑94 (MQ=60)

       CATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCC                                                                                         <  SRR4302537.1351453/94‑1 (MQ=60)

       CATCGACAAAAACTCCACTCTTGCCGCCGCCATCGGCTATCCCGATATGGTTTCGCTGTTTGCCGCCACCGTGCTGAATCAGACGGG‑G‑CAAGCC                                                                                         <  SRR4302537.3899081/94‑1 (MQ=60)

        ATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCA                                                                                        <  SRR4302537.1615849/94‑1 (MQ=60)

         TCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCAT                                                                                       >  SRR4302537.5744175/1‑94 (MQ=60)

         TCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCAT                                                                                       <  SRR4302537.2345964/94‑1 (MQ=60)

          CGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATC                                                                                      >  SRR4302537.2696274/1‑94 (MQ=60)

           GTCAAAAACTCCTCTCTTGCTGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCG                                                                                     >  SRR4302537.1725608/1‑94 (MQ=60)

           GTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCG                                                                                     <  SRR4302537.647237/94‑1 (MQ=60)

            TCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGA                                                                                    >  SRR4302537.6290389/1‑94 (MQ=60)

              AAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGA                                                                                  >  SRR4302537.2233184/1‑94 (MQ=60)

              AAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGA                                                                                  <  SRR4302537.2058817/94‑1 (MQ=60)

              AAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGA                                                                                  >  SRR4302537.5713463/1‑94 (MQ=60)

                  ACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGAT                                                                              >  SRR4302537.2003706/1‑94 (MQ=60)

                   CTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATA                                                                             <  SRR4302537.2233122/94‑1 (MQ=60)

                    TCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAG                                                                            >  SRR4302537.4454515/1‑94 (MQ=60)

                    TCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAG                                                                            <  SRR4302537.3812328/94‑1 (MQ=60)

                    TCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAG                                                                            <  SRR4302537.2801248/94‑1 (MQ=60)

                    TCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAG                                                                            <  SRR4302537.2223168/94‑1 (MQ=60)

                    TCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTATTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAG                                                                            <  SRR4302537.6123572/94‑1 (MQ=60)

                     CCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGC                                                                           >  SRR4302537.4007526/1‑94 (MQ=60)

                      CTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCC                                                                          >  SRR4302537.956991/1‑94 (MQ=60)

                      CTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCC                                                                          >  SRR4302537.4900642/1‑94 (MQ=60)

                      CTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCC                                                                          >  SRR4302537.6220162/1‑94 (MQ=60)

                      CTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCC                                                                          >  SRR4302537.2336296/1‑94 (MQ=60)

                        CTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCAT                                                                        <  SRR4302537.5927894/94‑1 (MQ=60)

                        CTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCAT                                                                        <  SRR4302537.321491/94‑1 (MQ=60)

                        CTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCAT                                                                        >  SRR4302537.3229236/1‑94 (MQ=60)

                          CTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGA                                                                      >  SRR4302537.3732957/1‑94 (MQ=60)

                          CTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGA                                                                      >  SRR4302537.2744461/1‑94 (MQ=60)

                          CTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGA                                                                      >  SRR4302537.1371378/1‑94 (MQ=60)

                           TTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGAC                                                                     >  SRR4302537.4463127/1‑94 (MQ=60)

                            TGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACC                                                                    <  SRR4302537.2294826/94‑1 (MQ=60)

                            TGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACC                                                                    <  SRR4302537.3743980/94‑1 (MQ=60)

                             GCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCA                                                                   <  SRR4302537.1120132/94‑1 (MQ=60)

                              CCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCAT                                                                  <  SRR4302537.5296976/94‑1 (MQ=60)

                               CGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATG                                                                 >  SRR4302537.5229423/1‑94 (MQ=60)

                                GCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGT                                                                >  SRR4302537.6267060/1‑94 (MQ=60)

                                 CCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTC                                                               <  SRR4302537.1854379/94‑1 (MQ=60)

                                 CCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GG‑GCCAAGCCATCGAGACGATAGCCATGACCATGTC                                                               <  SRR4302537.2545047/94‑1 (MQ=60)

                                  CGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCG                                                              >  SRR4302537.4429178/1‑94 (MQ=60)

                                   GCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGG                                                             >  SRR4302537.4993908/1‑94 (MQ=60)

                                    CTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCATACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGT                                                            >  SRR4302537.3732808/1‑94 (MQ=60)

                                    CTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGT                                                            <  SRR4302537.464359/94‑1 (MQ=60)

                                    CTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGT                                                            >  SRR4302537.2183351/1‑94 (MQ=60)

                                    CTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACAATGTCGGT                                                            >  SRR4302537.2028420/1‑94 (MQ=60)

                                    CTATGGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGT                                                            <  SRR4302537.657010/94‑1 (MQ=60)

                                     TATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTC                                                           <  SRR4302537.6063549/94‑1 (MQ=60)

                                      ATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCT                                                          <  SRR4302537.1904438/94‑1 (MQ=60)

                                       TTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTA                                                         <  SRR4302537.4484961/94‑1 (MQ=60)

                                        TGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCCTGTCGGTCTAT                                                        >  SRR4302537.2950150/1‑94 (MQ=60)

                                        TGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTAT                                                        <  SRR4302537.577274/94‑1 (MQ=60)

                                        TGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTAT                                                        >  SRR4302537.4457150/1‑94 (MQ=60)

                                         GGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATC                                                       <  SRR4302537.3708577/94‑1 (MQ=60)

                                          GCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCT                                                      <  SRR4302537.5225102/94‑1 (MQ=60)

                                          GCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCT                                                      <  SRR4302537.5603439/94‑1 (MQ=60)

                                          GCAATCCCGATATGGTTTCGCTGTTTGCCGGCACCATGGTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCT                                                      <  SRR4302537.6124013/94‑1 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                     <  SRR4302537.4645320/94‑1 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                     >  SRR4302537.765827/1‑94 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                     >  SRR4302537.1116949/1‑94 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                     <  SRR4302537.2148558/94‑1 (MQ=60)

                                            TATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGA                                                    >  SRR4302537.3610816/1‑94 (MQ=60)

                                            TATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGA                                                    <  SRR4302537.2824588/94‑1 (MQ=60)

                                            TATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGA                                                    >  SRR4302537.145744/1‑94 (MQ=60)

                                             ATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAT                                                   >  SRR4302537.2626807/1‑94 (MQ=60)

                                             ATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAT                                                   >  SRR4302537.1960704/1‑94 (MQ=60)

                                              TCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATT                                                  <  SRR4302537.349320/94‑1 (MQ=60)

                                              TCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATT                                                  <  SRR4302537.3978522/94‑1 (MQ=60)

                                               CCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTA                                                 <  SRR4302537.841111/94‑1 (MQ=60)

                                               CCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTA                                                 >  SRR4302537.4808869/1‑94 (MQ=60)

                                                CCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTAT                                                >  SRR4302537.6007219/1‑94 (MQ=60)

                                                CCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTAT                                                <  SRR4302537.5213864/94‑1 (MQ=60)

                                                CCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTAT                                                <  SRR4302537.2505375/94‑1 (MQ=60)

                                                 CGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATC                                               >  SRR4302537.2968161/1‑94 (MQ=60)

                                                  GATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCA                                              <  SRR4302537.132740/94‑1 (MQ=60)

                                                  GATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCA                                              <  SRR4302537.836614/94‑1 (MQ=60)

                                                   ATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGCCGATAGCCATGACCATGTCGGTCTATCTGATTATCAG                                             >  SRR4302537.1446058/1‑94 (MQ=60)

                                                   ATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGGCATGACCATGTCGGTCTATCTGATTATAAG                                             >  SRR4302537.1772173/1‑94 (MQ=60)

                                                   ATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAG                                             >  SRR4302537.2030886/1‑94 (MQ=60)

                                                   ATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAG                                             <  SRR4302537.255191/94‑1 (MQ=60)

                                                    TATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGC                                            <  SRR4302537.1343659/94‑1 (MQ=60)

                                                    TATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGC                                            >  SRR4302537.3337811/1‑94 (MQ=60)

                                                     ATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCC                                           >  SRR4302537.5520228/1‑94 (MQ=60)

                                                      TGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCT                                          >  SRR4302537.6461909/1‑94 (MQ=60)

                                                      TGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCT                                          <  SRR4302537.3457599/94‑1 (MQ=60)

                                                      TGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCT                                          <  SRR4302537.5655500/94‑1 (MQ=60)

                                                      TGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCT                                          >  SRR4302537.3436848/1‑94 (MQ=60)

                                                      TGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGTG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCT                                          <  SRR4302537.1271778/94‑1 (MQ=60)

                                                       GGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTG                                         >  SRR4302537.620882/1‑94 (MQ=60)

                                                        GTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGA                                        <  SRR4302537.1845094/94‑1 (MQ=60)

                                                          TTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACT                                      <  SRR4302537.4599010/94‑1 (MQ=60)

                                                          TTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACT                                      <  SRR4302537.2197931/94‑1 (MQ=60)

                                                          TTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACT                                      <  SRR4302537.1306587/94‑1 (MQ=60)

                                                          TGCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACT                                      <  SRR4302537.3770018/94‑1 (MQ=60)

                                                           TCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTA                                     <  SRR4302537.2950939/94‑1 (MQ=60)

                                                            CGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAT                                    <  SRR4302537.2614470/94‑1 (MQ=60)

                                                            CGCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAT                                    >  SRR4302537.3262766/1‑94 (MQ=60)

                                                             GCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATC                                   <  SRR4302537.2177378/94‑1 (MQ=60)

                                                             GCTGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATC                                   <  SRR4302537.3286775/94‑1 (MQ=60)

                                                               TGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                                 >  SRR4302537.6472374/1‑94 (MQ=60)

                                                               TGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                                 <  SRR4302537.2363994/94‑1 (MQ=60)

                                                               TGTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                                 >  SRR4302537.550770/1‑94 (MQ=60)

                                                                GTTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCG                                <  SRR4302537.2609714/94‑1 (MQ=60)

                                                                 TTTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGC                               <  SRR4302537.3657751/94‑1 (MQ=60)

                                                                  TTGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCT                              <  SRR4302537.1864394/94‑1 (MQ=60)

                                                                   TGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTG                             <  SRR4302537.6504522/94‑1 (MQ=60)

                                                                   TGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTG                             >  SRR4302537.6353001/1‑94 (MQ=60)

                                                                   TGCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGACAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTG                             <  SRR4302537.6257443/94‑1 (MQ=60)

                                                                    GCCGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGC                            >  SRR4302537.3802653/1‑94 (MQ=60)

                                                                      CGGCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTG                          <  SRR4302537.3691884/94‑1 (MQ=60)

                                                                        GCACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGAT                        <  SRR4302537.2874972/94‑1 (MQ=60)

                                                                          ACCGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGA                      <  SRR4302537.4335198/94‑1 (MQ=60)

                                                                            CGTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAAT                    >  SRR4302537.3995288/1‑94 (MQ=60)

                                                                             GTGCTGAATCAGACTGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATA                   <  SRR4302537.920129/94‑1 (MQ=60)

                                                                             GTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATA                   >  SRR4302537.5440515/1‑94 (MQ=60)

                                                                             GTGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATA                   <  SRR4302537.643617/94‑1 (MQ=60)

                                                                              TGCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATAT                  <  SRR4302537.3205133/94‑1 (MQ=60)

                                                                               GCTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTCATTATCAGCCTGACTATCTCGCTGCTGATGAATATT                 >  SRR4302537.3113254/1‑94 (MQ=60)

                                                                                CTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCT                <  SRR4302537.822364/94‑1 (MQ=60)

                                                                                CTGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCT                <  SRR4302537.1523268/94‑1 (MQ=60)

                                                                                 TGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGTCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTA               <  SRR4302537.5727340/94‑1 (MQ=60)

                                                                                 TGAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTA               >  SRR4302537.474384/1‑94 (MQ=60)

                                                                                  GAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTAT              <  SRR4302537.1398914/94‑1 (MQ=60)

                                                                                  GAATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTAT              >  SRR4302537.4828856/1‑94 (MQ=60)

                                                                                   AATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATA             <  SRR4302537.1501692/94‑1 (MQ=60)

                                                                                    ATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAA            >  SRR4302537.5095/1‑94 (MQ=60)

                                                                                    ATCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAA            <  SRR4302537.291014/94‑1 (MQ=60)

                                                                                     TCAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC           >  SRR4302537.6170094/1‑94 (MQ=60)

                                                                                      CAGACGGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACC          >  SRR4302537.6229711/1‑94 (MQ=60)

                                                                                         acgGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCC       <  SRR4302537.3711244/91‑1 (MQ=60)

                                                                                          cgGG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCG      <  SRR4302537.2582745/92‑1 (MQ=60)

                                                                                          G‑GG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGC     >  SRR4302537.1843955/1‑94 (MQ=60)

                                                                                            GG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCA    >  SRR4302537.1997351/1‑94 (MQ=60)

                                                                                             G‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCAT   >  SRR4302537.1650762/1‑94 (MQ=60)

                                                                                               G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATC  <  SRR4302537.643288/94‑1 (MQ=60)

                                                                                               G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATC  <  SRR4302537.2944048/94‑1 (MQ=60)

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GG‑G‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGCATC  >  NC_000913/3418747‑3418933

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`