Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,448,691 A→G I104T (ATC→ACC)  rplB ← 50S ribosomal subunit protein L2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,448,6910AG100.0% 775.6 / NA 221I104T (ATC→ACC) rplB50S ribosomal subunit protein L2
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (112/109);  total (112/109)

Only 100 of 229 total aligned reads displayed.

TTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGATGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGG  >  NC_000913/3448601‑3448779
                                                                                          |                                                                                        
ttGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTg                                                                                        <  1:2513383/93‑1 (MQ=255)
   cgcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTaa                                                                                     >  2:619459/1‑93 (MQ=255)
   cgcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTaa                                                                                     <  2:2242718/93‑1 (MQ=255)
   cgcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTaa                                                                                     <  1:2354477/93‑1 (MQ=255)
    gcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAAc                                                                                    <  1:1572479/93‑1 (MQ=255)
    gcATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAAc                                                                                    <  2:1490113/93‑1 (MQ=255)
       tCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGc                                                                                 <  1:2561304/93‑1 (MQ=255)
        cGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCg                                                                                <  1:3277958/93‑1 (MQ=255)
         ggCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGt                                                                               <  1:4990789/93‑1 (MQ=255)
          gCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGGGtt                                                                              >  2:1995623/1‑93 (MQ=255)
           cAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTc                                                                             <  2:1218530/93‑1 (MQ=255)
            aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCa                                                                            <  2:2411038/93‑1 (MQ=255)
            aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCa                                                                            >  2:1127557/1‑93 (MQ=255)
            aGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCa                                                                            <  1:880446/93‑1 (MQ=255)
             gggTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTccc                                                                           <  2:4163448/93‑3 (MQ=255)
             gggTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAc                                                                           <  1:415328/93‑1 (MQ=255)
             gggTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAc                                                                           <  1:4526839/93‑1 (MQ=255)
              ggTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAcc                                                                          <  1:1487910/93‑1 (MQ=255)
              ggTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAcc                                                                          <  1:6001280/93‑1 (MQ=255)
                 gtTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTc                                                                       <  2:3274573/93‑1 (MQ=255)
                  ttACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCt                                                                      >  1:394343/1‑93 (MQ=255)
                   tACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCtt                                                                     <  2:1423515/93‑1 (MQ=255)
                    aCCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCttt                                                                    >  1:399365/1‑93 (MQ=255)
                     ccTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTg                                                                   >  2:2991579/1‑93 (MQ=255)
                     ccTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTg                                                                   >  1:2270932/1‑93 (MQ=255)
                     ccTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTg                                                                   >  2:3686768/1‑93 (MQ=255)
                     ccTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTg                                                                   >  2:681855/1‑93 (MQ=255)
                      cTGGTTTGATTGCAGCATCAACGCCAGACTGAAACTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGt                                                                  >  1:1408865/1‑93 (MQ=255)
                       tGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTa                                                                 >  1:3477680/1‑93 (MQ=255)
                         gTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACa                                                               <  2:5067209/93‑1 (MQ=255)
                         gTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACa                                                               >  1:6071441/1‑93 (MQ=255)
                             gATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAAc                                                           >  2:5331365/1‑93 (MQ=255)
                              aTTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAAcc                                                          >  1:6394425/1‑93 (MQ=255)
                              aTTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAAcc                                                          <  1:1903772/93‑1 (MQ=255)
                              aTTGCAGCATCAACGCAAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAAcc                                                          <  2:610571/93‑1 (MQ=255)
                                tGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAg                                                        <  1:2677388/93‑1 (MQ=255)
                                tGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAg                                                        <  2:4218146/93‑1 (MQ=255)
                                tGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAg                                                        <  1:2998012/93‑1 (MQ=255)
                                  cagcaTCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAgcg                                                      <  2:2756851/93‑1 (MQ=255)
                                   agcaTCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAgcgc                                                     >  2:3092998/1‑93 (MQ=255)
                                     caTCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGa                                                   <  2:1852074/93‑1 (MQ=255)
                                       tCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATg                                                 >  1:1374932/1‑93 (MQ=255)
                                        cAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGt                                                <  2:6467943/93‑1 (MQ=255)
                                        cAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGt                                                <  1:6268126/93‑1 (MQ=255)
                                         aaCGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGtt                                               <  2:1547512/93‑1 (MQ=255)
                                            gCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTcgc                                            >  2:5475498/1‑93 (MQ=255)
                                            gCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTcgc                                            >  1:1963661/1‑93 (MQ=255)
                                               aGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGa                                         >  2:1016540/1‑93 (MQ=255)
                                               aGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGa                                         >  2:3498935/1‑93 (MQ=255)
                                                gACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGaa                                        >  1:5073862/1‑93 (MQ=255)
                                                gACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGaa                                        >  1:827175/1‑93 (MQ=255)
                                                 aCTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAAc                                       >  1:2957337/1‑93 (MQ=255)
                                                 aCTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAAc                                       <  1:1159810/93‑1 (MQ=255)
                                                  cTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACg                                      <  1:168744/93‑1 (MQ=255)
                                                  cTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGCGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGCACg                                      >  1:4306927/1‑93 (MQ=255)
                                                  cTGAATCTGGTCGCCAACTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACg                                      <  1:4862666/93‑1 (MQ=255)
                                                   tGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACgg                                     >  2:4660310/1‑93 (MQ=255)
                                                    gAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGt                                    >  2:1637229/1‑93 (MQ=255)
                                                    gAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGt                                    >  1:2896377/1‑93 (MQ=255)
                                                     aaTCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGtt                                   >  1:2448677/1‑93 (MQ=255)
                                                     aaTCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGtt                                   >  1:5373104/1‑93 (MQ=255)
                                                      aTCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTc                                  <  2:3382679/93‑1 (MQ=255)
                                                       tCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCg                                 >  2:1347338/1‑93 (MQ=255)
                                                         tGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGa                               >  1:2229008/1‑93 (MQ=255)
                                                         tGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGa                               >  2:3660151/1‑93 (MQ=255)
                                                         tGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGa                               >  1:3111498/1‑93 (MQ=255)
                                                           gTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATc                             <  2:2167768/93‑1 (MQ=255)
                                                            tCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTACGCGGAACGGTTCGGATCg                            <  2:6273453/93‑1 (MQ=255)
                                                              gCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAGCCAGCGCGATGTTCGCGGAACGGTTCGGATCGTa                          <  2:4571281/93‑1 (MQ=255)
                                                              gCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTa                          >  1:5216834/1‑93 (MQ=255)
                                                              gCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTa                          <  2:4288143/93‑1 (MQ=255)
                                                               ccAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTAc                         >  2:4100154/1‑93 (MQ=255)
                                                                   cTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCaa                     >  1:155355/1‑93 (MQ=255)
                                                                   cTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCaa                     >  1:561255/1‑93 (MQ=255)
                                                                    tttCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAg                    >  2:5435933/1‑93 (MQ=255)
                                                                      tCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGAc                  <  2:4199111/93‑1 (MQ=255)
                                                                        aGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGt                >  1:4699230/1‑93 (MQ=255)
                                                                        aGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGt                >  1:5349960/1‑93 (MQ=255)
                                                                        aGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGt                >  1:4036485/1‑93 (MQ=255)
                                                                        aGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGt                <  2:2019147/93‑1 (MQ=255)
                                                                         ggCCTTTAGGGGCCAGGGTGTAACGGCGTGCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGtt               <  2:2329230/93‑1 (MQ=255)
                                                                         ggCCTTGAGGGGCCAGGGTGTAACTGCGTCCACCGTCTTTGTACAGAACCAGCGCGATGCTCGCGGAACGGTTCGGATCGTACTCAAGACGtt               <  2:2638623/93‑1 (MQ=255)
                                                                          gCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTc              >  1:810116/1‑93 (MQ=255)
                                                                            cTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTcaa            >  1:4634808/1‑93 (MQ=255)
                                                                            cTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTcaa            >  1:3575212/1‑93 (MQ=255)
                                                                            atttAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTcaa            <  1:5161186/92‑1 (MQ=255)
                                                                             tttAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTcaac           >  1:547068/1‑93 (MQ=255)
                                                                             tttAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTcaac           >  2:5932838/1‑93 (MQ=255)
                                                                              ttAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTcaaca          <  1:6461715/93‑1 (MQ=255)
                                                                              ttAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTcaaca          >  2:386139/1‑93 (MQ=255)
                                                                               tAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTcaacaa         <  1:5928917/93‑1 (MQ=255)
                                                                                aGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCaacaac        <  2:4434016/93‑1 (MQ=255)
                                                                                aGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCaacaac        >  1:109234/1‑93 (MQ=255)
                                                                                aGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCaacaac        <  1:5115402/93‑1 (MQ=255)
                                                                                 ggggCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACt       >  1:338368/1‑93 (MQ=255)
                                                                                 ggggCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACt       <  1:4419453/93‑1 (MQ=255)
                                                                                   ggCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGc     <  1:3885519/93‑1 (MQ=255)
                                                                                    ggcAGGGTGTAACGGCGTTCCCCTTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTGCGGATCGTACTCAAGACGTTCAACAACTGcc    <  2:2445119/91‑1 (MQ=255)
                                                                                     ccAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCg   <  2:6302797/93‑1 (MQ=255)
                                                                                      cAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCgg  <  1:4942047/93‑1 (MQ=255)
                                                                                          |                                                                                        
TTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGATGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGG  >  NC_000913/3448601‑3448779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGATGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGATAC  >  NC_000913/3448598‑3448784
                                                                                             |                                                                                             
ATGTTGCGCATCGGCAGGGAGTTACCTGGATTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGG                                                                                               >  SRR4302537.2210354/1‑94 (MQ=60)
 TGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGT                                                                                              >  SRR4302537.2422189/1‑94 (MQ=60)
 TGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGT                                                                                              <  SRR4302537.1908065/94‑1 (MQ=60)
  GTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTG                                                                                             <  SRR4302537.6469582/94‑1 (MQ=60)
  GTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTG                                                                                             <  SRR4302537.2602939/94‑1 (MQ=60)
   TTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGT                                                                                            <  SRR4302537.2531052/94‑1 (MQ=60)
      CGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAAC                                                                                         <  SRR4302537.2371078/94‑1 (MQ=60)
      CGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAAC                                                                                         >  SRR4302537.139865/1‑94 (MQ=60)
       GCATCGGCAGTGTGTTACCTGATTTGATTGCAGCAACAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACG                                                                                        <  SRR4302537.1059647/94‑1 (MQ=60)
       GCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACG                                                                                        >  SRR4302537.2386798/1‑94 (MQ=60)
       GCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACG                                                                                        <  SRR4302537.2283292/94‑1 (MQ=60)
       GCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACG                                                                                        <  SRR4302537.1583451/94‑1 (MQ=60)
          TCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCG                                                                                     <  SRR4302537.2579247/94‑1 (MQ=60)
           CGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGT                                                                                    >  SRR4302537.5022651/1‑94 (MQ=60)
           CGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGT                                                                                    <  SRR4302537.3301095/94‑1 (MQ=60)
            GGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTT                                                                                   <  SRR4302537.5025182/94‑1 (MQ=60)
              CAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCA                                                                                 >  SRR4302537.4098802/1‑94 (MQ=60)
               AGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAC                                                                                >  SRR4302537.3000897/1‑94 (MQ=60)
               AGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAC                                                                                <  SRR4302537.1497026/94‑1 (MQ=60)
               AGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAC                                                                                <  SRR4302537.886505/94‑1 (MQ=60)
                GGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACC                                                                               <  SRR4302537.4558159/94‑1 (MQ=60)
                GGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACC                                                                               <  SRR4302537.418208/94‑1 (MQ=60)
                 GGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCG                                                                              <  SRR4302537.5915127/94‑1 (MQ=60)
                 GGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCG                                                                              <  SRR4302537.12351/94‑1 (MQ=60)
                 GGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCG                                                                              <  SRR4302537.6042503/94‑1 (MQ=60)
                 GGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCG                                                                              <  SRR4302537.1498262/94‑1 (MQ=60)
                  GTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGT                                                                             <  SRR4302537.5139742/94‑1 (MQ=60)
                   TGTTTCCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGTCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTC                                                                            <  SRR4302537.5495567/94‑1 (MQ=60)
                   TGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCAGCGTC                                                                            >  SRR4302537.5165504/1‑94 (MQ=60)
                   TGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTC                                                                            <  SRR4302537.3090293/94‑1 (MQ=60)
                    GTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCT                                                                           >  SRR4302537.397076/1‑94 (MQ=60)
                     TTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTT                                                                          <  SRR4302537.2951078/94‑1 (MQ=60)
                     TTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTT                                                                          >  SRR4302537.4028549/1‑94 (MQ=60)
                      TACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTT                                                                         <  SRR4302537.5336955/94‑1 (MQ=60)
                      TACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTT                                                                         >  SRR4302537.6345961/1‑94 (MQ=60)
                      TACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTT                                                                         >  SRR4302537.402134/1‑94 (MQ=60)
                       ACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTG                                                                        >  SRR4302537.2286967/1‑94 (MQ=60)
                        CCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGT                                                                       <  SRR4302537.824094/94‑1 (MQ=60)
                        CCTGGTTTGATTGCAGCATCAACGCCAGACTGAAACTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGT                                                                       >  SRR4302537.1418680/1‑94 (MQ=60)
                         CTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTA                                                                      <  SRR4302537.4455940/94‑1 (MQ=60)
                         CTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTA                                                                      >  SRR4302537.3502290/1‑94 (MQ=60)
                           GGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACA                                                                    >  SRR4302537.4727420/1‑94 (MQ=60)
                           GGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACA                                                                    >  SRR4302537.6113157/1‑94 (MQ=60)
                            GTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAG                                                                   <  SRR4302537.1745516/94‑1 (MQ=60)
                            GTTCGATTGCAGCACCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTATAGGTGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAG                                                                   <  SRR4302537.4075539/94‑1 (MQ=60)
                              TTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAA                                                                 >  SRR4302537.6031234/1‑94 (MQ=60)
                               TGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAAC                                                                <  SRR4302537.2819800/94‑1 (MQ=60)
                                GATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACC                                                               >  SRR4302537.6437977/1‑94 (MQ=60)
                                GATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACC                                                               <  SRR4302537.2490009/94‑1 (MQ=60)
                                 ATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCA                                                              <  SRR4302537.1916992/94‑1 (MQ=60)
                                 ATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCA                                                              >  SRR4302537.333495/1‑94 (MQ=60)
                                   TGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGC                                                            <  SRR4302537.3664963/94‑1 (MQ=60)
                                   TGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGC                                                            <  SRR4302537.2696085/94‑1 (MQ=60)
                                   TGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGC                                                            <  SRR4302537.3018912/94‑1 (MQ=60)
                                      AGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCTGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCG                                                         >  SRR4302537.2235322/1‑94 (MQ=60)
                                      AGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGAG                                                         >  SRR4302537.399048/1‑94 (MQ=60)
                                        CATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGAT                                                       >  SRR4302537.4387015/1‑94 (MQ=60)
                                         ATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATG                                                      <  SRR4302537.318938/94‑1 (MQ=60)
                                         ATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATG                                                      >  SRR4302537.1384531/1‑94 (MQ=60)
                                           CAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTT                                                    <  SRR4302537.6311148/94‑1 (MQ=60)
                                           CAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTACGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTT                                                    <  SRR4302537.2968254/94‑1 (MQ=60)
                                              CGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGC                                                 >  SRR4302537.1977315/1‑94 (MQ=60)
                                                CCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGCTCGCGG                                               >  SRR4302537.3543148/1‑94 (MQ=60)
                                                  AGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAA                                             >  SRR4302537.4049361/1‑94 (MQ=60)
                                                  AGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAA                                             >  SRR4302537.832840/1‑94 (MQ=60)
                                                  AGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAA                                             >  SRR4302537.5108792/1‑94 (MQ=60)
                                                  AGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAAACAGCGCGATGCTCGCGGAA                                             >  SRR4302537.5546212/1‑94 (MQ=60)
                                                   GACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAAC                                            >  SRR4302537.2978087/1‑94 (MQ=60)
                                                   GACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAAC                                            <  SRR4302537.2687176/94‑1 (MQ=60)
                                                    ACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACG                                           <  SRR4302537.5295978/94‑1 (MQ=60)
                                                    ACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACG                                           <  SRR4302537.1167879/94‑1 (MQ=60)
                                                    ACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGCGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGCACG                                           >  SRR4302537.4336864/1‑94 (MQ=60)
                                                     CTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGG                                          <  SRR4302537.169947/94‑1 (MQ=60)
                                                     CTGAATCTGGTCGCCAACTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGG                                          <  SRR4302537.4896214/94‑1 (MQ=60)
                                                      TGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGT                                         >  SRR4302537.5198667/1‑94 (MQ=60)
                                                      TGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGT                                         >  SRR4302537.2916708/1‑94 (MQ=60)
                                                      TGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGCTCGCGGAACGGT                                         >  SRR4302537.6451154/1‑94 (MQ=60)
                                                       GAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTT                                        >  SRR4302537.5409854/1‑94 (MQ=60)
                                                       GAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTT                                        >  SRR4302537.2465902/1‑94 (MQ=60)
                                                         ATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCG                                      <  SRR4302537.5070604/94‑1 (MQ=60)
                                                         ATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCG                                      <  SRR4302537.5070603/94‑1 (MQ=60)
                                                         ATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCG                                      >  SRR4302537.2561202/1‑94 (MQ=60)
                                                         ATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCG                                      <  SRR4302537.318217/94‑1 (MQ=60)
                                                          TCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGG                                     >  SRR4302537.2319853/1‑94 (MQ=60)
                                                           CTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGA                                    >  SRR4302537.3848108/1‑94 (MQ=60)
                                                           CTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGA                                    >  SRR4302537.2244723/1‑94 (MQ=60)
                                                           CTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGA                                    >  SRR4302537.3133229/1‑94 (MQ=60)
                                                             GGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAGCGGGTCGGATT                                  >  SRR4302537.5594017/1‑94 (MQ=60)
                                                             GGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATC                                  <  SRR4302537.1577180/94‑1 (MQ=60)
                                                              GTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCG                                 <  SRR4302537.4189184/94‑1 (MQ=60)
                                                               TCGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGT                                >  SRR4302537.4573500/1‑94 (MQ=60)
                                                                CGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTA                               >  SRR4302537.5252709/1‑94 (MQ=60)
                                                                CGCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTA                               >  SRR4302537.845250/1‑94 (MQ=60)
                                                                 GCCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTAC                              <  SRR4302537.6068962/94‑1 (MQ=60)
                                                                  CCAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACT                             <  SRR4302537.3462081/94‑1 (MQ=60)
                                                                   CAGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTC                            >  SRR4302537.5394772/1‑94 (MQ=60)
                                                                    AGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCA                           >  SRR4302537.4889741/1‑94 (MQ=60)
                                                                    AGCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCA                           >  SRR4302537.1436799/1‑94 (MQ=60)
                                                                     GCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAA                          <  SRR4302537.6506932/94‑1 (MQ=60)
                                                                     GCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAA                          >  SRR4302537.565118/1‑94 (MQ=60)
                                                                     GCTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAA                          >  SRR4302537.156459/1‑94 (MQ=60)
                                                                      CTTTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAG                         <  SRR4302537.1640285/94‑1 (MQ=60)
                                                                        TTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGAC                       >  SRR4302537.4747718/1‑94 (MQ=60)
                                                                        TTCAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGAC                       >  SRR4302537.1639337/1‑94 (MQ=60)
                                                                          CAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGT                     >  SRR4302537.1101175/1‑94 (MQ=60)
                                                                          CAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGT                     >  SRR4302537.5386613/1‑94 (MQ=60)
                                                                          CAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGT                     >  SRR4302537.4731741/1‑94 (MQ=60)
                                                                          CAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGT                     >  SRR4302537.4064733/1‑94 (MQ=60)
                                                                          CAGGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGT                     >  SRR4302537.4545677/1‑94 (MQ=60)
                                                                            GGCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTC                   >  SRR4302537.815667/1‑94 (MQ=60)
                                                                             GCCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCA                  <  SRR4302537.6190703/94‑1 (MQ=60)
                                                                              CCTTTAGGGGCCAGGGTGTAGCGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAA                 <  SRR4302537.4947874/94‑1 (MQ=60)
                                                                              CCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAA                 >  SRR4302537.3600424/1‑94 (MQ=60)
                                                                              CCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAA                 <  SRR4302537.1931137/94‑1 (MQ=60)
                                                                              CCTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAA                 >  SRR4302537.4666868/1‑94 (MQ=60)
                                                                               CTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAAC                >  SRR4302537.2282317/1‑94 (MQ=60)
                                                                               CTTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAAC                >  SRR4302537.550837/1‑94 (MQ=60)
                                                                               CTTTAGGGGCCAGGGTGTAACGGCGTTCAACGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAAC                >  SRR4302537.1208514/1‑94 (MQ=60)
                                                                               ATTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAAC                <  SRR4302537.5196768/94‑1 (MQ=60)
                                                                                TTTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACA               <  SRR4302537.3933495/94‑1 (MQ=60)
                                                                                 TTAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAA              <  SRR4302537.6505500/94‑1 (MQ=60)
                                                                                  TAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAAC             <  SRR4302537.5969602/94‑1 (MQ=60)
                                                                                  TAGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAAC             >  SRR4302537.110035/1‑94 (MQ=60)
                                                                                   AGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACT            >  SRR4302537.340715/1‑94 (MQ=60)
                                                                                   AGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACT            <  SRR4302537.2470215/94‑1 (MQ=60)
                                                                                   AGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACT            <  SRR4302537.910408/94‑1 (MQ=60)
                                                                                   AGGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACT            <  SRR4302537.5150660/94‑1 (MQ=60)
                                                                                    GGGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTG           <  SRR4302537.4449966/94‑1 (MQ=60)
                                                                                     GGGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGC          <  SRR4302537.799219/94‑1 (MQ=60)
                                                                                      GGCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCC         <  SRR4302537.3912699/94‑1 (MQ=60)
                                                                                       GCCAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCG        >  SRR4302537.6282018/1‑94 (MQ=60)
                                                                                         CAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGG      <  SRR4302537.4976128/94‑1 (MQ=60)
                                                                                         CAGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGG      <  SRR4302537.1555677/94‑1 (MQ=60)
                                                                                          AGGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGA     >  SRR4302537.1022616/1‑94 (MQ=60)
                                                                                           GGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGAT    <  SRR4302537.57801/94‑1 (MQ=60)
                                                                                           GGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGAT    <  SRR4302537.5118571/94‑1 (MQ=60)
                                                                                           GGGTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGAT    >  SRR4302537.221986/1‑94 (MQ=60)
                                                                                             GTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGATAC  <  SRR4302537.4460349/94‑1 (MQ=60)
                                                                                             GTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGATAC  >  SRR4302537.5475318/1‑94 (MQ=60)
                                                                                             GTGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGATAC  <  SRR4302537.1524854/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATGTTGCGCATCGGCAGGGTGTTACCTGGTTTGATTGCAGCATCAACGCCAGACTGAATCTGGTCGCCAGCTTTCAGGCCTTTAGGGGCCAGGATGTAACGGCGTTCACCGTCTTTGTACAGAACCAGCGCGATGTTCGCGGAACGGTTCGGATCGTACTCAAGACGTTCAACAACTGCCGGGATAC  >  NC_000913/3448598‑3448784

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑