Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,855,776	G→A	R86C (CGC→TGC)	yidJ ←	putative sulfatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,855,776	0	G	A	99.2%	913.1 / NA	267	R86C (CGC→TGC)	yidJ	putative sulfatase
Reads supporting (aligned to +/- strand): ref base G (0/1); major base A (142/122); minor base C (0/2); total (142/125)
Fisher's exact test for biased strand distribution p-value = 2.16e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 269 total aligned reads displayed.

GCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCC  >  NC_000913/3855686‑3855865

gcggACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCAcc                                                                                         <  2:5632696/93‑1 (MQ=255)

  ggACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCAcccc                                                                                       <  1:1786366/93‑1 (MQ=255)

     cacTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCAccccttg                                                                                    >  1:540553/1‑90 (MQ=255)

      acTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAg                                                                                   >  2:1744355/1‑93 (MQ=255)

      acTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAg                                                                                   <  1:5520177/93‑1 (MQ=255)

       cTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGt                                                                                  >  1:549554/1‑93 (MQ=255)

       cTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGt                                                                                  <  1:3575377/93‑1 (MQ=255)

         cGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTgg                                                                                <  2:3867465/93‑1 (MQ=255)

             aGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGAt                                                                            <  2:3431761/93‑1 (MQ=255)

             aGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGAt                                                                            <  2:1466366/93‑1 (MQ=255)

             aGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGAt                                                                            <  1:3390958/93‑1 (MQ=255)

              gTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATg                                                                           <  1:674356/93‑1 (MQ=255)

               tGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGt                                                                          >  1:6062894/1‑93 (MQ=255)

               tGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGt                                                                          <  1:3294851/93‑1 (MQ=255)

               tGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGt                                                                          <  2:927598/93‑1 (MQ=255)

                gCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGtt                                                                         <  2:4822297/93‑1 (MQ=255)

                gCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGtt                                                                         >  2:2983569/1‑93 (MQ=255)

                 ccGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGttt                                                                        <  2:708947/93‑1 (MQ=255)

                 ccGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGttt                                                                        >  1:5190553/1‑93 (MQ=255)

                   gAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGttttt                                                                      <  2:4393734/93‑1 (MQ=255)

                      aTAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGcc                                                                   >  2:5963268/1‑93 (MQ=255)

                       tAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCt                                                                  >  1:2405590/1‑93 (MQ=255)

                       tAGTCATGACCGACGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCCCCCCATAGTGGAGATGTTTTTGCCt                                                                  <  2:3920555/93‑1 (MQ=255)

                         gTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTgg                                                                <  2:1400982/93‑1 (MQ=255)

                          tCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGgc                                                               <  1:61939/93‑1 (MQ=255)

                            aTGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGgcgc                                                             >  2:5860398/1‑93 (MQ=255)

                             tGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGcgcg                                                            >  2:2443177/1‑93 (MQ=255)

                              gACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGa                                                           >  1:1482472/1‑93 (MQ=255)

                                ccgccgAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCGGGCGCGACg                                                         <  2:4308203/93‑1 (MQ=255)

                                   tCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACgttg                                                      >  1:457731/1‑93 (MQ=255)

                                   tCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACgttg                                                      >  1:4732125/1‑93 (MQ=255)

                                    cGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACgttgt                                                     <  2:4536956/93‑1 (MQ=255)

                                     gagaTGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACgttgtt                                                    >  2:2904670/1‑93 (MQ=255)

                                     gagaTGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACgttgtt                                                    >  1:5784725/1‑93 (MQ=255)

                                     gagaTGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACgttgtt                                                    >  2:2226427/1‑93 (MQ=255)

                                     gagaTGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACgttgtt                                                    <  1:4718507/93‑1 (MQ=255)

                                      agaTGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGttgttg                                                   <  2:3806490/93‑1 (MQ=255)

                                       gaTGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTgg                                                  <  2:6371287/93‑1 (MQ=255)

                                        aTGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGt                                                 >  2:2789036/1‑93 (MQ=255)

                                         tGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTc                                                >  2:3063793/1‑93 (MQ=255)

                                          gCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTcc                                               >  2:619580/1‑93 (MQ=255)

                                           ccATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCa                                              >  2:4896376/1‑93 (MQ=255)

                                            cATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCAc                                             >  2:2961093/1‑93 (MQ=255)

                                            cATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCAc                                             >  2:6117753/1‑93 (MQ=255)

                                            cATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCAc                                             <  1:35369/93‑1 (MQ=255)

                                             aTTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACg                                            <  1:3084320/93‑1 (MQ=255)

                                             aTTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACg                                            >  2:5462517/1‑93 (MQ=255)

                                              tttGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACgg                                           >  2:752283/1‑93 (MQ=255)

                                              tttGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACgg                                           <  2:4255262/93‑1 (MQ=255)

                                              tttGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACgg                                           >  2:2325686/1‑93 (MQ=255)

                                                tGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGc                                         >  2:452014/1‑93 (MQ=255)

                                                 gCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGcc                                        <  2:1954422/93‑1 (MQ=255)

                                                 gCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGcc                                        >  1:2952044/1‑93 (MQ=255)

                                                   cGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCgg                                      >  2:1647090/1‑93 (MQ=255)

                                                     aTGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGAc                                    <  1:4761961/93‑1 (MQ=255)

                                                     aTGAAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGGCGGAc                                    >  2:31882/1‑93 (MQ=255)

                                                      tGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACt                                   <  1:280261/93‑1 (MQ=255)

                                                      tGTAACAGGTGTGATAGCCGGCAGCCTGAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACt                                   <  2:2610643/93‑1 (MQ=255)

                                                         aaCAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGt                                <  1:5053988/93‑1 (MQ=255)

                                                          aCAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGtt                               >  1:1737663/1‑93 (MQ=255)

                                                          aCAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGtt                               >  2:5066933/1‑93 (MQ=255)

                                                            aGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAg                             >  1:1515005/1‑93 (MQ=255)

                                                            aGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAg                             >  1:5595487/1‑93 (MQ=255)

                                                            aGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAg                             >  1:1993030/1‑93 (MQ=255)

                                                            aGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAg                             >  2:2485981/1‑93 (MQ=255)

                                                            aGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAg                             >  1:4209986/1‑93 (MQ=255)

                                                             ggTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGc                            <  2:4176361/93‑1 (MQ=255)

                                                             ggTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGc                            >  1:1206551/1‑93 (MQ=255)

                                                             ggTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGc                            >  1:753841/1‑93 (MQ=255)

                                                             ggTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGc                            >  1:5257874/1‑93 (MQ=255)

                                                             ggTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGc                            <  1:5308090/93‑1 (MQ=255)

                                                             ggTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCAACGGGCCGGACTGGTTAGc                            >  2:5914965/1‑93 (MQ=255)

                                                             ggTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCAACGGGCCGGACTGGTTAGc                            >  1:3842773/1‑93 (MQ=255)

                                                                gtgATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTa                         <  2:3559265/93‑1 (MQ=255)

                                                                gtgATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTa                         >  2:1357953/1‑93 (MQ=255)

                                                                gtgATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTa                         >  1:4867952/1‑93 (MQ=255)

                                                                 tgATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAg                        >  1:1417023/1‑93 (MQ=255)

                                                                   aTAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGAt                      <  1:1614448/93‑1 (MQ=255)

                                                                   aTAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGAt                      >  1:2727592/1‑93 (MQ=255)

                                                                      gCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATAcc                   >  1:6323278/1‑93 (MQ=255)

                                                                      gCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATAcc                   <  1:4338830/93‑1 (MQ=255)

                                                                      gCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATAcc                   >  2:2039320/1‑93 (MQ=255)

                                                                       ccGGCATCCTTAAAGTAGCCCCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCg                  <  2:6299892/93‑1 (MQ=255)

                                                                       ccGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCg                  >  1:2278741/1‑93 (MQ=255)

                                                                        cGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCgg                 >  2:153421/1‑93 (MQ=255)

                                                                         ggCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGt                >  1:2001790/1‑93 (MQ=255)

                                                                          gCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTa               >  2:857927/1‑93 (MQ=255)

                                                                          gCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTa               >  1:4001879/1‑93 (MQ=255)

                                                                            aTCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCCGTaaa             >  2:5367290/1‑93 (MQ=255)

                                                                             tCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAAt            >  1:911919/1‑93 (MQ=255)

                                                                             tCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAAt            >  2:5210080/1‑93 (MQ=255)

                                                                             tCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAAt            <  1:2905012/93‑1 (MQ=255)

                                                                               cTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCTGGACTGGTTAGCGTAGATACCGGTAAATAg          <  1:1873716/93‑1 (MQ=255)

                                                                                 tAAAGTAGCACCCCATAGTGGAGAAGTTTTTGCCTGGCGCGACGTTGTTGGTACACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTc        <  1:6470890/93‑1 (MQ=255)

                                                                                  aaaGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTcc       <  2:3212462/93‑1 (MQ=255)

                                                                                   aaGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCg      <  2:1118889/93‑1 (MQ=255)

                                                                                     gTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGc    >  2:1812605/1‑93 (MQ=255)

                                                                                     gTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGc    >  1:318102/1‑93 (MQ=255)

                                                                                      tAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGcc   >  1:4997141/1‑93 (MQ=255)

                                                                                       aGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGccc  >  1:1025189/1‑93 (MQ=255)

GCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCC  >  NC_000913/3855686‑3855865

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGC  >  NC_000913/3855683‑3855869

CCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCA                                                                                               >  SRR4302537.5006719/1‑94 (MQ=60)

CCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCA                                                                                               >  SRR4302537.6001137/1‑94 (MQ=60)

 CGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCAC                                                                                              <  SRR4302537.1015056/94‑1 (MQ=60)

 CGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCAC                                                                                              >  SRR4302537.3367444/1‑94 (MQ=60)

 CGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCAC                                                                                              <  SRR4302537.525333/94‑1 (MQ=60)

 CGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGAGGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCAC                                                                                              >  SRR4302537.4576539/1‑94 (MQ=60)

   GCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCC                                                                                            >  SRR4302537.5513508/1‑94 (MQ=60)

   GCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCC                                                                                            <  SRR4302537.5533744/94‑1 (MQ=60)

     GGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCA                                                                                          <  SRR4302537.1798825/94‑1 (MQ=60)

        cacctgttacatCCGAAATAGTCATGACCGTCGAGATGCCATTGGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAG                                                                                       <  SRR4302537.3250016/82‑1 (MQ=60)

        CACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAG                                                                                       <  SRR4302537.5462198/94‑1 (MQ=60)

        CACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAG                                                                                       <  SRR4302537.4749061/94‑1 (MQ=60)

         ACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGT                                                                                      >  SRR4302537.553337/1‑94 (MQ=60)

         ACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGT                                                                                      <  SRR4302537.5557864/94‑1 (MQ=60)

          CTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTG                                                                                     <  SRR4302537.6413546/94‑1 (MQ=60)

          CTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTG                                                                                     <  SRR4302537.3600590/94‑1 (MQ=60)

            CGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGA                                                                                   <  SRR4302537.5213890/94‑1 (MQ=60)

            CGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGA                                                                                   >  SRR4302537.1531713/1‑94 (MQ=60)

               CAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGAT                                                                                >  SRR4302537.1454509/1‑94 (MQ=60)

               CAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGAT                                                                                >  SRR4302537.3998677/1‑94 (MQ=60)

                AGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATG                                                                               <  SRR4302537.5056654/94‑1 (MQ=60)

                AGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATG                                                                               <  SRR4302537.3414979/94‑1 (MQ=60)

                 GTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGT                                                                              >  SRR4302537.6104550/1‑94 (MQ=60)

                 GTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGT                                                                              <  SRR4302537.678972/94‑1 (MQ=60)

                  TGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTT                                                                             <  SRR4302537.3318138/94‑1 (MQ=60)

                  TGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTT                                                                             >  SRR4302537.5734655/1‑94 (MQ=60)

                  TGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTT                                                                             <  SRR4302537.5607382/94‑1 (MQ=60)

                  TGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTT                                                                             <  SRR4302537.1346148/94‑1 (MQ=60)

                   GCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTT                                                                            <  SRR4302537.2112796/94‑1 (MQ=60)

                   GCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTT                                                                            >  SRR4302537.5226255/1‑94 (MQ=60)

                    CCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTT                                                                           <  SRR4302537.6259288/94‑1 (MQ=60)

                    CCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTT                                                                           >  SRR4302537.4762417/1‑94 (MQ=60)

                       AAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGC                                                                        <  SRR4302537.1903884/94‑1 (MQ=60)

                         ATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCT                                                                      <  SRR4302537.1840003/94‑1 (MQ=60)

                         ATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCT                                                                      <  SRR4302537.336248/94‑1 (MQ=60)

                         ATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCT                                                                      >  SRR4302537.2422523/1‑94 (MQ=60)

                         ATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCT                                                                      >  SRR4302537.5156289/1‑94 (MQ=60)

                           TGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATGGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGG                                                                    <  SRR4302537.6517051/94‑1 (MQ=60)

                           AGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGG                                                                    >  SRR4302537.4573156/1‑94 (MQ=60)

                            GTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGC                                                                   >  SRR4302537.4321040/1‑94 (MQ=60)

                            GTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGC                                                                   >  SRR4302537.1924708/1‑94 (MQ=60)

                             TCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCG                                                                  <  SRR4302537.62401/94‑1 (MQ=60)

                                TGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGA                                                               >  SRR4302537.1492783/1‑94 (MQ=60)

                                 GACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGAC                                                              <  SRR4302537.2943405/94‑1 (MQ=60)

                                     GTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTG                                                          <  SRR4302537.1691823/94‑1 (MQ=60)

                                     GTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTG                                                          >  SRR4302537.460887/1‑94 (MQ=60)

                                     GTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTG                                                          >  SRR4302537.4764821/1‑94 (MQ=60)

                                     GTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTG                                                          >  SRR4302537.1865781/1‑94 (MQ=60)

                                       CGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTT                                                        >  SRR4302537.5824383/1‑94 (MQ=60)

                                        GAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTG                                                       <  SRR4302537.4751132/94‑1 (MQ=60)

                                        GAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTG                                                       <  SRR4302537.5318592/94‑1 (MQ=60)

                                        GAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTG                                                       <  SRR4302537.2420665/94‑1 (MQ=60)

                                          GATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGT                                                     >  SRR4302537.3591626/1‑94 (MQ=60)

                                           ATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTC                                                    <  SRR4302537.6203772/94‑1 (MQ=60)

                                           ATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTC                                                    <  SRR4302537.6287595/94‑1 (MQ=60)

                                           ATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTC                                                    >  SRR4302537.1523076/1‑94 (MQ=60)

                                             GCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCA                                                  >  SRR4302537.1785017/1‑94 (MQ=60)

                                              GCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCAC                                                 <  SRR4302537.4957697/94‑1 (MQ=60)

                                              CCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCAC                                                 >  SRR4302537.2758039/1‑94 (MQ=60)

                                               CATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACG                                                >  SRR4302537.345134/1‑94 (MQ=60)

                                               CATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACG                                                <  SRR4302537.35642/94‑1 (MQ=60)

                                                ATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGG                                               <  SRR4302537.3105808/94‑1 (MQ=60)

                                                 TTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGG                                              <  SRR4302537.1725362/94‑1 (MQ=60)

                                                   TGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCC                                            >  SRR4302537.6194610/1‑94 (MQ=60)

                                                   TGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCC                                            >  SRR4302537.2972763/1‑94 (MQ=60)

                                                    GCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCG                                           <  SRR4302537.2007194/94‑1 (MQ=60)

                                                    GCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCG                                           <  SRR4302537.312272/94‑1 (MQ=60)

                                                     CCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGG                                          <  SRR4302537.1387381/94‑1 (MQ=60)

                                                       GATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGAC                                        >  SRR4302537.6057450/1‑94 (MQ=60)

                                                        ATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACT                                       <  SRR4302537.4794850/94‑1 (MQ=60)

                                                         TGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTG                                      <  SRR4302537.282217/94‑1 (MQ=60)

                                                         TGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTG                                      >  SRR4302537.3658951/1‑94 (MQ=60)

                                                          GTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGG                                     <  SRR4302537.735072/94‑1 (MQ=60)

                                                           TAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTGGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGT                                    <  SRR4302537.638036/94‑1 (MQ=60)

                                                            AACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTT                                   >  SRR4302537.1749781/1‑94 (MQ=60)

                                                            AACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTT                                   <  SRR4302537.5088783/94‑1 (MQ=60)

                                                            AACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTT                                   >  SRR4302537.2936794/1‑94 (MQ=60)

                                                            AACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTT                                   >  SRR4302537.2334883/1‑94 (MQ=60)

                                                            AACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTT                                   <  SRR4302537.4145963/94‑1 (MQ=60)

                                                             ACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTA                                  >  SRR4302537.4565398/1‑94 (MQ=60)

                                                             ACAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTA                                  <  SRR4302537.3934943/94‑1 (MQ=60)

                                                              CAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAG                                 >  SRR4302537.2006887/1‑94 (MQ=60)

                                                              CAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAG                                 >  SRR4302537.1525592/1‑94 (MQ=60)

                                                              CAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAG                                 >  SRR4302537.4239354/1‑94 (MQ=60)

                                                              CAGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAG                                 >  SRR4302537.5633748/1‑94 (MQ=60)

                                                               AGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGC                                >  SRR4302537.5294057/1‑94 (MQ=60)

                                                               AGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGC                                >  SRR4302537.542464/1‑94 (MQ=60)

                                                               AGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGC                                >  SRR4302537.1214948/1‑94 (MQ=60)

                                                               AGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGC                                >  SRR4302537.758981/1‑94 (MQ=60)

                                                               AGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGC                                <  SRR4302537.78618/94‑1 (MQ=60)

                                                               AGGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCAACGGGCCGGACTGGTTAGC                                >  SRR4302537.3869684/1‑94 (MQ=60)

                                                                GGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCG                               >  SRR4302537.1479243/1‑94 (MQ=60)

                                                                GGTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCG                               <  SRR4302537.5344562/94‑1 (MQ=60)

                                                                 GTGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGT                              <  SRR4302537.4870295/94‑1 (MQ=60)

                                                                  TGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTA                             >  SRR4302537.4901542/1‑94 (MQ=60)

                                                                  TGTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTA                             <  SRR4302537.2897120/94‑1 (MQ=60)

                                                                   GTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAG                            >  SRR4302537.1426887/1‑94 (MQ=60)

                                                                   GTGATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAG                            <  SRR4302537.5778356/94‑1 (MQ=60)

                                                                     GATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGAT                          <  SRR4302537.4780052/94‑1 (MQ=60)

                                                                     GATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGAT                          >  SRR4302537.2055284/1‑94 (MQ=60)

                                                                     GATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGAT                          >  SRR4302537.2746636/1‑94 (MQ=60)

                                                                      ATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATA                         <  SRR4302537.1625729/94‑1 (MQ=60)

                                                                      ATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATA                         <  SRR4302537.5439188/94‑1 (MQ=60)

                                                                      ATAGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATA                         >  SRR4302537.3033123/1‑94 (MQ=60)

                                                                        AGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACC                       >  SRR4302537.6366540/1‑94 (MQ=60)

                                                                        AGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACC                       <  SRR4302537.3465281/94‑1 (MQ=60)

                                                                        AGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACC                       >  SRR4302537.3575905/1‑94 (MQ=60)

                                                                        AGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACC                       >  SRR4302537.3178132/1‑94 (MQ=60)

                                                                        AGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACC                       <  SRR4302537.4862853/94‑1 (MQ=60)

                                                                        AGCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTCTTGGTCCACGGGCCGGACCGGTTAGCGTAGATACC                       >  SRR4302537.4644254/1‑94 (MQ=60)

                                                                         GCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCG                      >  SRR4302537.2294827/1‑94 (MQ=60)

                                                                         GCCGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCG                      <  SRR4302537.4368941/94‑1 (MQ=60)

                                                                           CGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGT                    >  SRR4302537.2015708/1‑94 (MQ=60)

                                                                           CGGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGT                    >  SRR4302537.5000709/1‑94 (MQ=60)

                                                                            GGCATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTA                   >  SRR4302537.4029895/1‑94 (MQ=60)

                                                                               ATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAAT                <  SRR4302537.742999/94‑1 (MQ=60)

                                                                               ATCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAAT                >  SRR4302537.918209/1‑94 (MQ=60)

                                                                                TCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATA               <  SRR4302537.1577704/94‑1 (MQ=60)

                                                                                TCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATA               <  SRR4302537.5241005/94‑1 (MQ=60)

                                                                                TCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATA               <  SRR4302537.523010/94‑1 (MQ=60)

                                                                                TCCTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATA               <  SRR4302537.2925422/94‑1 (MQ=60)

                                                                                  CTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCTGGACTGGTTAGCGTAGATACCGGTAAATAGT             <  SRR4302537.1886754/94‑1 (MQ=60)

                                                                                  CTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGT             <  SRR4302537.4757552/94‑1 (MQ=60)

                                                                                  CTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGT             >  SRR4302537.829567/1‑94 (MQ=60)

                                                                                  CTTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGT             >  SRR4302537.2819305/1‑94 (MQ=60)

                                                                                   TTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGTCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTC            <  SRR4302537.1110163/94‑1 (MQ=60)

                                                                                   TTAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTC            >  SRR4302537.3240872/1‑94 (MQ=60)

                                                                                    TAAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCC           >  SRR4302537.2903137/1‑94 (MQ=60)

                                                                                    TAAAGTAGCACCCCATAGTGGAGAAGTTTTTGCCTGGCGCGACGTTGTTGGTACACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCC           <  SRR4302537.6514764/94‑1 (MQ=60)

                                                                                     AAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGGGACGTTGTTGGTCCACGGGCCGGGCTGGGTAGCGGAGATAACGGTAAATAGTCCG          >  SRR4302537.2520657/1‑94 (MQ=60)

                                                                                     AAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCG          <  SRR4302537.3270189/94‑1 (MQ=60)

                                                                                     AAAGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCG          <  SRR4302537.5603603/94‑1 (MQ=60)

                                                                                       AGTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGC        >  SRR4302537.320307/1‑94 (MQ=60)

                                                                                        GTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCC       <  SRR4302537.4150158/94‑1 (MQ=60)

                                                                                        GTAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCC       >  SRR4302537.5031576/1‑94 (MQ=60)

                                                                                         TAGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCC      >  SRR4302537.1032242/1‑94 (MQ=60)

                                                                                          AGCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTCAATAGTCCGGCCCG     >  SRR4302537.1689245/1‑94 (MQ=60)

                                                                                           GCACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGT    <  SRR4302537.4556768/94‑1 (MQ=60)

                                                                                             ACCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGC  >  SRR4302537.5503897/1‑94 (MQ=60)

CCGGCGGACACTCGCCAGTGCCGAAATAGTCATGACCGTCGAGATGCCATTTGCCGATGTAACAGGTGTGATAGCCGGCATCCTTAAAGTAGCGCCCCATAGTGGAGATGTTTTTGCCTGGCGCGACGTTGTTGGTCCACGGGCCGGACTGGTTAGCGTAGATACCGGTAAATAGTCCGGCCCGTGC  >  NC_000913/3855683‑3855869

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`