Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,856,065	G→A	A560V (GCC→GTC)	yidK ←	putative cotransporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,856,065	0	G	A	94.5%	806.8 / 17.6	238	A560V (GCC→GTC)	yidK	putative cotransporter
Reads supporting (aligned to +/- strand): ref base G (10/3); new base A (105/120); total (115/123)
Fisher's exact test for biased strand distribution p-value = 4.49e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.05e-01

Only 100 of 246 total aligned reads displayed.

GACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCGCGTA  >  NC_000913/3855975‑3856157

gACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACt                                                                                            >  1:2469591/1‑93 (MQ=255)

  ccATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTGATAGGCTATCCTTCCCGTCGGGAGGAAAGGTTAAGACTgg                                                                                          >  1:1157726/1‑93 (MQ=255)

  ccATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTgg                                                                                          <  1:5963268/93‑1 (MQ=255)

  ccATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTgg                                                                                          <  1:859677/93‑1 (MQ=255)

   cATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTggg                                                                                         >  1:3175856/1‑93 (MQ=255)

     tatTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTc                                                                                       >  1:1091214/1‑93 (MQ=255)

     catTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTc                                                                                       <  1:6383218/92‑1 (MQ=255)

      atTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCg                                                                                      >  2:4056780/1‑93 (MQ=255)

       ttGGTGGCCTGGGAATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGGTACGACTGGGGCgg                                                                                     >  2:2850113/1‑92 (MQ=255)

        tggtggCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTg                                                                                    <  1:5697139/93‑1 (MQ=255)

        tggtggCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTg                                                                                    <  2:5505019/93‑1 (MQ=255)

        tggtggCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTg                                                                                    <  2:4657643/93‑1 (MQ=255)

        tggtggCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTg                                                                                    <  2:3527522/93‑1 (MQ=255)

          gtggCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGAc                                                                                  <  2:4531851/93‑1 (MQ=255)

           tggCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACg                                                                                 >  1:4074874/1‑93 (MQ=255)

              ccTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATg                                                                              <  1:4333899/93‑1 (MQ=255)

              ccTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATg                                                                              >  1:4977465/1‑93 (MQ=255)

              ccTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATg                                                                              >  1:3632386/1‑93 (MQ=255)

               cTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATgc                                                                             <  2:5257874/93‑1 (MQ=255)

                tGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGCCGATgcc                                                                            >  1:4659901/1‑93 (MQ=255)

                tGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATgcc                                                                            <  1:6269450/93‑1 (MQ=255)

                tGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATgcc                                                                            <  2:5737223/93‑1 (MQ=255)

                tGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATgcc                                                                            >  2:814177/1‑93 (MQ=255)

                    tATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCa                                                                        <  2:3218228/93‑1 (MQ=255)

                      tCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGc                                                                      >  2:302225/1‑93 (MQ=255)

                        ggTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTg                                                                    >  1:5247862/1‑93 (MQ=255)

                         gTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGt                                                                   >  1:1563627/1‑93 (MQ=255)

                          tCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTc                                                                  <  1:2106405/93‑1 (MQ=255)

                             tGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCaaa                                                               >  2:1246008/1‑93 (MQ=255)

                             tGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCaaa                                                               >  2:328640/1‑93 (MQ=255)

                             tGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCaaa                                                               <  2:5878415/93‑1 (MQ=255)

                             tGACGAACAGAAAATTGGGGCGTTTAATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCaaa                                                               >  1:3582884/1‑93 (MQ=255)

                              gACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCaaaa                                                              >  2:44666/1‑93 (MQ=255)

                               aCGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCaaaaa                                                             <  2:2318511/93‑1 (MQ=255)

                                 gAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATc                                                           <  1:2263407/93‑1 (MQ=255)

                                 gAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGGAAAGGTTACGACTGGGTCGTGACGATGCCGCCCGCTGGCAAAAATc                                                           >  2:830123/1‑93 (MQ=255)

                                  aaCAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCa                                                          <  2:6004741/93‑1 (MQ=255)

                                  aaCAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCa                                                          >  2:2555011/1‑93 (MQ=255)

                                  aaCAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCa                                                          >  2:3939665/1‑93 (MQ=255)

                                  aaCAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCa                                                          <  2:423413/93‑1 (MQ=255)

                                  aaCAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCa                                                          >  1:3793081/1‑93 (MQ=255)

                                    cAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAgg                                                        >  1:3252030/1‑93 (MQ=255)

                                     aGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGt                                                       >  1:2751028/1‑93 (MQ=255)

                                      gAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTa                                                      <  1:2723850/93‑1 (MQ=255)

                                      gAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTa                                                      <  2:1677503/93‑1 (MQ=255)

                                      gAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTa                                                      >  1:670910/1‑93 (MQ=255)

                                      gAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTa                                                      <  1:287118/93‑1 (MQ=255)

                                      gAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTa                                                      >  2:3675498/1‑93 (MQ=255)

                                       aaaaTTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAg                                                     >  2:4756310/1‑93 (MQ=255)

                                       aaaaTTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAg                                                     <  2:342754/93‑1 (MQ=255)

                                         aaTTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGAc                                                   <  2:5085541/93‑1 (MQ=255)

                                         aaTTGCGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGAc                                                   <  2:1295970/93‑1 (MQ=255)

                                           ttGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACaa                                                 >  1:3073599/1‑93 (MQ=255)

                                            tGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAAt                                                <  1:3390776/93‑1 (MQ=255)

                                               ggCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCAc                                             <  1:5421012/93‑1 (MQ=255)

                                               ggCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCAc                                             >  1:3335226/1‑93 (MQ=255)

                                               ggCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCAc                                             <  2:4966480/93‑1 (MQ=255)

                                               ggCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCACGTAGACAATCAc                                             <  1:3486525/93‑1 (MQ=255)

                                                   tttCATAGGCTATCCTTCCCGTCGGGAGTCAAGGCTACGACTGGGTCGTGACGATGCCGCCAGCTGTGAAAAATCAGGTAGACAATCACTAcc                                         >  1:167771/1‑92 (MQ=255)

                                                   tttCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTAcg                                         >  1:3112566/1‑93 (MQ=255)

                                                   tttCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTAcg                                         >  1:5127844/1‑93 (MQ=255)

                                                   tttCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTAcg                                         <  1:1357953/93‑1 (MQ=255)

                                                    ttCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTAcgg                                        <  1:848071/93‑1 (MQ=255)

                                                    ttCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTAcgg                                        >  1:2142772/1‑93 (MQ=255)

                                                      cATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTAcggcg                                      >  2:2920973/1‑93 (MQ=255)

                                                       aTAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTAcggcgg                                     <  2:318102/93‑1 (MQ=255)

                                                        tAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACggcggc                                    >  1:4433998/1‑93 (MQ=255)

                                                        tAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACggcggc                                    <  1:74388/93‑1 (MQ=255)

                                                         aGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCa                                   >  1:1168916/1‑93 (MQ=255)

                                                         aGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCa                                   <  1:2021637/93‑1 (MQ=255)

                                                          ggCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCaa                                  >  2:632875/1‑93 (MQ=255)

                                                            cTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAAGCAGACAATCACTAAGGCGGCAAta                                >  2:1147783/1‑92 (MQ=255)

                                                              aTCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGaa                              >  1:503927/1‑93 (MQ=255)

                                                               tCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGaaa                             <  1:1332214/93‑1 (MQ=255)

                                                                ccTCCCCGTCGGGAGTAAAGTTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAAt                            <  2:2278741/93‑1 (MQ=255)

                                                                 cTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATa                           >  1:3870603/1‑93 (MQ=255)

                                                                 cTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATa                           >  1:1781073/1‑93 (MQ=255)

                                                                  ttCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATaa                          <  1:1396646/93‑1 (MQ=255)

                                                                  ttCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATaa                          >  2:5574655/1‑93 (MQ=255)

                                                                  ttCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATaa                          >  2:5423421/1‑93 (MQ=255)

                                                                   tCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCGGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAAc                         <  1:4772017/93‑1 (MQ=255)

                                                                   tCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAAc                         <  1:5363021/93‑1 (MQ=255)

                                                                   tCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAAc                         >  2:6358574/1‑93 (MQ=255)

                                                                    cccGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACt                        <  2:5387665/93‑1 (MQ=255)

                                                                    cccGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACt                        >  2:1259742/1‑93 (MQ=255)

                                                                     ccGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTg                       <  1:994723/93‑1 (MQ=255)

                                                                      cGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGa                      <  1:4168781/93‑1 (MQ=255)

                                                                       gTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGat                     <  1:2943621/93‑1 (MQ=255)

                                                                          gggAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGatcat                  >  1:388840/1‑93 (MQ=255)

                                                                           ggAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGatcatc                 <  2:2001790/93‑1 (MQ=255)

                                                                           ggAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGatcatc                 >  2:2746323/1‑93 (MQ=255)

                                                                           ggAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGatcatc                 <  2:1512506/93‑1 (MQ=255)

                                                                            gAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCg                <  2:2732520/93‑1 (MQ=255)

                                                                             aGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGc               <  2:5175565/93‑1 (MQ=255)

                                                                             aGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGc               <  2:5688328/93‑1 (MQ=255)

                                                                               tAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTa             >  1:5567017/1‑93 (MQ=255)

                                                                                aaaGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTaa            <  1:3726995/93‑1 (MQ=255)

                                                                                 aaGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAAc           >  2:3876531/1‑93 (MQ=255)

                                                                                 aaGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAAc           >  2:5456740/1‑93 (MQ=255)

                                                                                          gCTGGGTCGTGACGATGCCGCGAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGcgcggc  >  1:5324433/1‑91 (MQ=255)

GACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCGCGTA  >  NC_000913/3855975‑3856157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCGCGTAC  >  NC_000913/3855972‑3856158

ACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGA                                                                                               >  SRR4302537.3538778/1‑94 (MQ=60)

 CCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGAC                                                                                              >  SRR4302537.3653514/1‑94 (MQ=60)

  CGACCATATTGGTGTCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACT                                                                                             <  SRR4302537.2732698/94‑1 (MQ=60)

  CGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACT                                                                                             >  SRR4302537.2486953/1‑94 (MQ=60)

  CGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACT                                                                                             <  SRR4302537.5252733/94‑1 (MQ=60)

   GACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTG                                                                                            <  SRR4302537.6369448/94‑1 (MQ=60)

    GCCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGG                                                                                           <  SRR4302537.1694840/94‑1 (MQ=60)

    ACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTGATAGGCTATCCTTCCCGTCGGGAGGAAAGGTTAAGACTGG                                                                                           >  SRR4302537.1165781/1‑94 (MQ=60)

    ACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGG                                                                                           <  SRR4302537.117947/94‑1 (MQ=60)

    ACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGG                                                                                           >  SRR4302537.3811371/1‑94 (MQ=60)

     CCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGG                                                                                          <  SRR4302537.865576/94‑1 (MQ=60)

     CCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGG                                                                                          >  SRR4302537.3107867/1‑94 (MQ=60)

     CCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGG                                                                                          <  SRR4302537.6004198/94‑1 (MQ=60)

     CCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGG                                                                                          >  SRR4302537.3198176/1‑94 (MQ=60)

       ATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTC                                                                                        >  SRR4302537.1098750/1‑94 (MQ=60)

       ATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTC                                                                                        <  SRR4302537.6347374/94‑1 (MQ=60)

        TATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCG                                                                                       <  SRR4302537.2925075/94‑1 (MQ=60)

        TATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCG                                                                                       <  SRR4302537.59553/94‑1 (MQ=60)

        TATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCG                                                                                       <  SRR4302537.757411/94‑1 (MQ=60)

        CATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCG                                                                                       <  SRR4302537.6426728/94‑1 (MQ=60)

           TGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGA                                                                                    <  SRR4302537.5736127/94‑1 (MQ=60)

            GGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGAC                                                                                   <  SRR4302537.1508840/94‑1 (MQ=60)

             GTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACG                                                                                  >  SRR4302537.4103325/1‑94 (MQ=60)

             GTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACG                                                                                  <  SRR4302537.1174199/94‑1 (MQ=60)

                GCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATG                                                                               >  SRR4302537.3657978/1‑94 (MQ=60)

                GCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATG                                                                               >  SRR4302537.5011771/1‑94 (MQ=60)

                 CCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGC                                                                              <  SRR4302537.4364000/94‑1 (MQ=60)

                  CTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGCCGATGCC                                                                             >  SRR4302537.4692115/1‑94 (MQ=60)

                  CTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCC                                                                             >  SRR4302537.5505744/1‑94 (MQ=60)

                   TGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCG                                                                            >  SRR4302537.1634859/1‑94 (MQ=60)

                   TGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCG                                                                            <  SRR4302537.6312477/94‑1 (MQ=60)

                     GGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCC                                                                          >  SRR4302537.3680585/1‑94 (MQ=60)

                      GTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCA                                                                         <  SRR4302537.2766127/94‑1 (MQ=60)

                       TATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAG                                                                        <  SRR4302537.6164445/94‑1 (MQ=60)

                        ATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGC                                                                       <  SRR4302537.3535531/94‑1 (MQ=60)

                        ATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGC                                                                       >  SRR4302537.1199643/1‑94 (MQ=60)

                         TCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCT                                                                      <  SRR4302537.6501134/94‑1 (MQ=60)

                         TCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCT                                                                      <  SRR4302537.743416/94‑1 (MQ=60)

                          CGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTG                                                                     >  SRR4302537.3430639/1‑94 (MQ=60)

                          CGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTG                                                                     <  SRR4302537.4862370/94‑1 (MQ=60)

                          CGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTG                                                                     >  SRR4302537.5283975/1‑94 (MQ=60)

                           GGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGT                                                                    >  SRR4302537.1845419/1‑94 (MQ=60)

                           GGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGT                                                                    >  SRR4302537.1574536/1‑94 (MQ=60)

                           GGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGT                                                                    >  SRR4302537.3163834/1‑94 (MQ=60)

                           GGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGT                                                                    >  SRR4302537.1145747/1‑94 (MQ=60)

                             TCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCA                                                                  <  SRR4302537.2121254/94‑1 (MQ=60)

                               ATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAA                                                                <  SRR4302537.3360034/94‑1 (MQ=60)

                               ATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAA                                                                >  SRR4302537.4153077/1‑94 (MQ=60)

                               ATGACGAACAGAAAATTGGGGCGTTTAATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAA                                                                >  SRR4302537.3608139/1‑94 (MQ=60)

                                    GAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGCTGCCGCCAGCTGTCAAAAATCA                                                           >  SRR4302537.1402850/1‑94 (MQ=60)

                                    GAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCA                                                           >  SRR4302537.3819703/1‑94 (MQ=60)

                                    GAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCA                                                           <  SRR4302537.2279381/94‑1 (MQ=60)

                                    GAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCA                                                           >  SRR4302537.4654587/1‑94 (MQ=60)

                                    GAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCA                                                           <  SRR4302537.5206881/94‑1 (MQ=60)

                                    GAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCA                                                           >  SRR4302537.5031613/1‑94 (MQ=60)

                                     AACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAG                                                          >  SRR4302537.3612254/1‑94 (MQ=60)

                                     AACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAG                                                          <  SRR4302537.5004571/94‑1 (MQ=60)

                                      ACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGG                                                         >  SRR4302537.4458684/1‑94 (MQ=60)

                                      ACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGG                                                         >  SRR4302537.5399678/1‑94 (MQ=60)

                                      ACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGG                                                         >  SRR4302537.3274955/1‑94 (MQ=60)

                                      ACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGG                                                         <  SRR4302537.2185610/94‑1 (MQ=60)

                                       CAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGT                                                        >  SRR4302537.2770223/1‑94 (MQ=60)

                                        AGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTA                                                       >  SRR4302537.675496/1‑94 (MQ=60)

                                         GAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAG                                                      <  SRR4302537.289117/94‑1 (MQ=60)

                                         GAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAG                                                      <  SRR4302537.2742875/94‑1 (MQ=60)

                                           AAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGAC                                                    >  SRR4302537.4245155/1‑94 (MQ=60)

                                           AAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGAC                                                    >  SRR4302537.926278/1‑94 (MQ=60)

                                             ATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAA                                                  >  SRR4302537.3000753/1‑94 (MQ=60)

                                             ATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAA                                                  >  SRR4302537.3095014/1‑94 (MQ=60)

                                              TTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTCACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAAT                                                 <  SRR4302537.4544117/94‑1 (MQ=60)

                                               TGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATC                                                <  SRR4302537.3414796/94‑1 (MQ=60)

                                               TGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATC                                                >  SRR4302537.5593877/1‑94 (MQ=60)

                                               TGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATC                                                <  SRR4302537.2145880/94‑1 (MQ=60)

                                                 GGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCAC                                              >  SRR4302537.3358827/1‑94 (MQ=60)

                                                  GGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACT                                             <  SRR4302537.5457952/94‑1 (MQ=60)

                                                  GGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCACGTAGACAATCACT                                             <  SRR4302537.3511182/94‑1 (MQ=60)

                                                    CGTTTCATAGGCTATCCTTCCCGTCGGGGGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAACAATCAGGTAGACAATCACTCC                                           >  SRR4302537.2844434/1‑94 (MQ=60)

                                                     GTTTCATAGGCTATCCTTCCCGTCGGGAGTCAAGGCTACGACTGGGTCGTGACGATGCCGCCAGCTGTGAAAAATCAGGTAGACAATCACTACC                                          >  SRR4302537.168967/1‑94 (MQ=60)

                                                     GTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACG                                          >  SRR4302537.3134306/1‑94 (MQ=60)

                                                     GTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACG                                          >  SRR4302537.5163184/1‑94 (MQ=60)

                                                     GTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACG                                          >  SRR4302537.3320674/1‑94 (MQ=60)

                                                      TTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGG                                         <  SRR4302537.3147035/94‑1 (MQ=60)

                                                      TTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGG                                         >  SRR4302537.2157848/1‑94 (MQ=60)

                                                      TTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGG                                         <  SRR4302537.1367408/94‑1 (MQ=60)

                                                       TTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGC                                        <  SRR4302537.853880/94‑1 (MQ=60)

                                                        TCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCG                                       >  SRR4302537.3901754/1‑94 (MQ=60)

                                                         CATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGG                                      <  SRR4302537.5953603/94‑1 (MQ=60)

                                                         CATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGG                                      <  SRR4302537.2013207/94‑1 (MQ=60)

                                                          ATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGC                                     <  SRR4302537.2705601/94‑1 (MQ=60)

                                                          ATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGC                                     >  SRR4302537.4464615/1‑94 (MQ=60)

                                                           TAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCA                                    >  SRR4302537.1177050/1‑94 (MQ=60)

                                                           TAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCA                                    <  SRR4302537.74934/94‑1 (MQ=60)

                                                            AGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAA                                   <  SRR4302537.2035744/94‑1 (MQ=60)

                                                            AGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAA                                   <  SRR4302537.2623574/94‑1 (MQ=60)

                                                            AGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGAGGCAA                                   >  SRR4302537.5186057/1‑94 (MQ=60)

                                                             GGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAAT                                  >  SRR4302537.3713658/1‑94 (MQ=60)

                                                             GGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAAT                                  <  SRR4302537.2079736/94‑1 (MQ=60)

                                                             GGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAAT                                  <  SRR4302537.5395397/94‑1 (MQ=60)

                                                             GGCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAAT                                  >  SRR4302537.476196/1‑94 (MQ=60)

                                                              GCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATG                                 <  SRR4302537.1599191/94‑1 (MQ=60)

                                                              GCTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATG                                 <  SRR4302537.2123735/94‑1 (MQ=60)

                                                               CTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGA                                >  SRR4302537.5640154/1‑94 (MQ=60)

                                                               CTATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGA                                >  SRR4302537.73751/1‑94 (MQ=60)

                                                                TATCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAA                               >  SRR4302537.507391/1‑94 (MQ=60)

                                                                  TCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAAT                             <  SRR4302537.1341499/94‑1 (MQ=60)

                                                                  TCCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAAT                             >  SRR4302537.2823518/1‑94 (MQ=60)

                                                                   CCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATA                            >  SRR4302537.1793485/1‑94 (MQ=60)

                                                                   CCTTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATA                            >  SRR4302537.3897687/1‑94 (MQ=60)

                                                                     TTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAAC                          <  SRR4302537.5077914/94‑1 (MQ=60)

                                                                     TTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAAC                          <  SRR4302537.1406380/94‑1 (MQ=60)

                                                                     TTCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAAC                          <  SRR4302537.3878995/94‑1 (MQ=60)

                                                                      TCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCGGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACT                         <  SRR4302537.4804965/94‑1 (MQ=60)

                                                                      TCCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACT                         <  SRR4302537.5399733/94‑1 (MQ=60)

                                                                       CCCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTG                        <  SRR4302537.2985452/94‑1 (MQ=60)

                                                                        CCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGA                       <  SRR4302537.1001591/94‑1 (MQ=60)

                                                                        CCGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGA                       <  SRR4302537.5438172/94‑1 (MQ=60)

                                                                         CGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGAT                      <  SRR4302537.5346349/94‑1 (MQ=60)

                                                                         CGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGAT                      <  SRR4302537.895750/94‑1 (MQ=60)

                                                                         CGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGAT                      >  SRR4302537.4239411/1‑94 (MQ=60)

                                                                         CGTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGAT                      <  SRR4302537.4197900/94‑1 (MQ=60)

                                                                          GTCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATC                     <  SRR4302537.2964283/94‑1 (MQ=60)

                                                                           TCGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCA                    <  SRR4302537.3838673/94‑1 (MQ=60)

                                                                            CGGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCAT                   >  SRR4302537.391527/1‑94 (MQ=60)

                                                                             GGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATC                  >  SRR4302537.3458604/1‑94 (MQ=60)

                                                                             GGGAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATC                  >  SRR4302537.135482/1‑94 (MQ=60)

                                                                               GAGTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGC                >  SRR4302537.2359056/1‑94 (MQ=60)

                                                                                 GTAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTA              >  SRR4302537.5605068/1‑94 (MQ=60)

                                                                                  TAAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAA             <  SRR4302537.3290369/94‑1 (MQ=60)

                                                                                  TAAAGATTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAA             <  SRR4302537.856900/94‑1 (MQ=60)

                                                                                   AAAGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAAC            <  SRR4302537.3753178/94‑1 (MQ=60)

                                                                                     AGGTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCA          <  SRR4302537.1367823/94‑1 (MQ=60)

                                                                                       GTTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGC        >  SRR4302537.3970419/1‑94 (MQ=60)

                                                                                        TTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCG       <  SRR4302537.4040086/94‑1 (MQ=60)

                                                                                        TTACGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCG       <  SRR4302537.32135/94‑1 (MQ=60)

                                                                                           CGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCGCGT    >  SRR4302537.4033676/1‑94 (MQ=60)

                                                                                           CGACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCGCGT    >  SRR4302537.2880080/1‑94 (MQ=60)

                                                                                            GGCTGGGTCGTGACGATGCCGCGAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCGCGgc   >  SRR4302537.5360971/1‑92 (MQ=60)

                                                                                            GACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCGCGTA   >  SRR4302537.144832/1‑94 (MQ=60)

                                                                                            GACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCGCGTA   >  SRR4302537.6249230/1‑94 (MQ=60)

                                                                                            GACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCGCGTA   <  SRR4302537.2179810/94‑1 (MQ=60)

                                                                                             ACTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCGCGTAC  <  SRR4302537.1060429/94‑1 (MQ=60)

ACCGACCATATTGGTGGCCTGGGTATCGGTCATGACGAACAGAAAATTGGGGCGTTTCATAGGCTATCCTTCCCGTCGGGAGTAAAGGTTACGGCTGGGTCGTGACGATGCCGCCAGCTGTCAAAAATCAGGTAGACAATCACTACGGCGGCAATGAAATAACTGATCATCGCTAACCAGCGCGTAC  >  NC_000913/3855972‑3856158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 15 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`