Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,856,327 C→T V473I (GTC→ATC)  yidK ← putative cotransporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,856,3270CT96.1% 883.2 / 12.7 255V473I (GTC→ATC) yidKputative cotransporter
Reads supporting (aligned to +/- strand):  ref base C (4/6);  new base T (113/131);  total (117/138)
Fisher's exact test for biased strand distribution p-value = 7.57e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.12e-01

Only 100 of 270 total aligned reads displayed.

TTTTTCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATGC  >  NC_000913/3856236‑3856416
                                                                                           |                                                                                         
tttttCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGAcc                                                                                          >  2:968398/1‑93 (MQ=255)
 ttttCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCa                                                                                         <  2:1063737/93‑1 (MQ=255)
   ttCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCatcacgatcacg                                                                                       >  2:2058310/1‑93 (MQ=255)
   ttCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCatcacgatcacg                                                                                       >  1:5624309/1‑93 (MQ=255)
    tCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGt                                                                                      <  1:5318907/93‑1 (MQ=255)
     ccACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGtt                                                                                     <  2:3627946/93‑1 (MQ=255)
     ccACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGtt                                                                                     <  2:3060990/93‑1 (MQ=255)
       aCGGTTTCATGTCCACCGCAAACGGATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGa                                                                                   >  2:4515699/1‑93 (MQ=255)
       aCGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGa                                                                                   <  2:162470/93‑1 (MQ=255)
        cGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGAt                                                                                  <  1:5141149/93‑1 (MQ=255)
         ggTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATg                                                                                 <  2:1897945/93‑1 (MQ=255)
         ggTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATg                                                                                 <  1:758950/93‑1 (MQ=255)
          gTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGc                                                                                <  1:6352553/93‑1 (MQ=255)
             tCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGa                                                                             >  2:1679981/1‑93 (MQ=255)
              cATGTCCACCACAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGaa                                                                            >  1:5010425/1‑93 (MQ=255)
               aTGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAAc                                                                           <  2:3434297/93‑1 (MQ=255)
                tGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACg                                                                          <  1:302225/93‑1 (MQ=255)
                  tCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTa                                                                        >  2:1258047/1‑93 (MQ=255)
                  tCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTa                                                                        >  2:2811954/1‑93 (MQ=255)
                  tCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTa                                                                        >  1:2874096/1‑93 (MQ=255)
                  tCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTa                                                                        <  1:2722696/93‑1 (MQ=255)
                   ccACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTAc                                                                       >  2:1716709/1‑93 (MQ=255)
                    cACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCTCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACa                                                                      <  1:53673/93‑1 (MQ=255)
                    cACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACa                                                                      <  1:933975/93‑1 (MQ=255)
                     aCCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAg                                                                     >  1:3695433/1‑93 (MQ=255)
                     aCCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAg                                                                     >  1:583062/1‑93 (MQ=255)
                     aCCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACAAGCATCACGATCACGTTGATGCAGAACGTACAg                                                                     >  1:711503/1‑93 (MQ=255)
                       cGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGc                                                                   <  2:2241945/93‑1 (MQ=255)
                       cGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGc                                                                   <  2:5601855/93‑1 (MQ=255)
                         cAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCa                                                                 >  2:6382697/1‑93 (MQ=255)
                         cAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCa                                                                 >  2:4752061/1‑93 (MQ=255)
                          aaaCGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGcccg                                                                >  1:1170147/1‑91 (MQ=255)
                            aCGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGca                                                              <  2:5449498/93‑1 (MQ=255)
                              gCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGcaca                                                            <  1:6179145/93‑1 (MQ=255)
                               cATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACAt                                                           >  1:6441757/1‑93 (MQ=255)
                               cATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACAt                                                           <  1:2450914/93‑1 (MQ=255)
                               cATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACAt                                                           >  1:6196865/1‑93 (MQ=255)
                                aTCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATa                                                          <  2:5285573/93‑1 (MQ=255)
                                 tCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATag                                                         >  1:4895592/1‑93 (MQ=255)
                                  cTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATaga                                                        <  1:4825365/93‑1 (MQ=255)
                                    ttGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAgg                                                      <  2:4671591/93‑1 (MQ=255)
                                       aaGTTGTACGGCGTAGCGCGCGGTTCGATAAACCCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATCGAGGaaa                                                   <  2:2503051/93‑1 (MQ=255)
                                       aaGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGaaa                                                   <  2:1168916/93‑1 (MQ=255)
                                        aGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAAt                                                  >  2:5104293/1‑93 (MQ=255)
                                         ggTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATg                                                 <  2:5050761/93‑1 (MQ=255)
                                          gTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATgg                                                <  2:2687779/93‑1 (MQ=255)
                                           tGAACGGCGTGGCGCGCGGTTTGATAAACCCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGa                                               <  2:2731782/93‑1 (MQ=255)
                                            gAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACACGCCAGCACATAGAGGAAATGGaa                                              >  1:1604921/1‑93 (MQ=255)
                                              aCGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGt                                            <  2:4349194/93‑1 (MQ=255)
                                              aCGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGt                                            <  1:5773560/93‑1 (MQ=255)
                                               cGGCGTGGCGCGCGGGTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTc                                           >  2:3617380/1‑93 (MQ=255)
                                                 gCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGa                                         >  1:4326416/1‑93 (MQ=255)
                                                 gCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACAGAGAGGAAATGGAAGTCGa                                         <  1:1754231/93‑1 (MQ=255)
                                                   gTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAAc                                       >  1:1029912/1‑93 (MQ=255)
                                                   gTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAAc                                       <  2:1781073/93‑1 (MQ=255)
                                                   gTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAAc                                       <  2:3160060/93‑1 (MQ=255)
                                                     ggCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACtt                                     <  1:4690654/93‑1 (MQ=255)
                                                         cgcgGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCAcc                                 <  1:855729/93‑1 (MQ=255)
                                                           cgGTTTGATTAAACCGATCACCAGCATCACGATCACGTTAATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAg                               >  1:2227419/1‑93 (MQ=255)
                                                           cgGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAg                               <  1:3270232/93‑1 (MQ=255)
                                                           cgGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAg                               <  1:3358239/93‑1 (MQ=255)
                                                            ggTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGa                              >  1:1538833/1‑93 (MQ=255)
                                                            ggTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGa                              <  2:212400/93‑1 (MQ=255)
                                                            ggTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGa                              <  2:143810/93‑1 (MQ=255)
                                                             gTTTGATAAAAACGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGAt                             >  2:1982617/1‑93 (MQ=255)
                                                              tttGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATa                            >  1:2054426/1‑93 (MQ=255)
                                                                tGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGt                          <  2:3870603/93‑1 (MQ=255)
                                                                tGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGt                          <  1:2482910/93‑1 (MQ=255)
                                                                 gATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGtt                         >  1:2115552/1‑93 (MQ=255)
                                                                   tAAAACCGATCACCAGCCTCACGATCACGTTGATGCAGAACGGACGGGCCAGCACATAGAAGTAAAATAAGTCGAACTTCACCCGATAATTGa                       >  2:3355368/1‑93 (MQ=255)
                                                                   tAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGa                       >  1:2210776/1‑93 (MQ=255)
                                                                    aaaaCCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGAt                      <  2:2179331/93‑1 (MQ=255)
                                                                    aaaaCCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGAt                      <  2:349536/93‑1 (MQ=255)
                                                                     aaaCCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATg                     <  2:2342550/93‑1 (MQ=255)
                                                                      aaCCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATgg                    <  1:1321332/93‑1 (MQ=255)
                                                                      aaCCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATgg                    >  2:5874481/1‑93 (MQ=255)
                                                                       aCCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGGAATGGAAGTCGAACTTCACCAGATAGTTGATGGt                   <  2:1500619/93‑1 (MQ=255)
                                                                          gATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGAt                <  1:5574655/93‑1 (MQ=255)
                                                                          gATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGAt                <  2:1310387/93‑1 (MQ=255)
                                                                           aTCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATg               >  2:4189679/1‑93 (MQ=255)
                                                                           aTCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATg               <  2:6003279/93‑1 (MQ=255)
                                                                           aTCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGGGATg               >  1:4415894/1‑93 (MQ=255)
                                                                            tCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGt              >  1:2364570/1‑93 (MQ=255)
                                                                            tCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGt              <  2:4024639/93‑1 (MQ=255)
                                                                             cACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTa             <  1:1729653/93‑1 (MQ=255)
                                                                              cccAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAg            <  2:3972435/92‑1 (MQ=255)
                                                                               ccAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGc           >  1:1966261/1‑93 (MQ=255)
                                                                               ccAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGc           >  1:3848869/1‑93 (MQ=255)
                                                                                cAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCt          >  1:3632704/1‑93 (MQ=255)
                                                                                  gCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGa        >  1:2840782/1‑93 (MQ=255)
                                                                                  gCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGa        >  1:5450656/1‑93 (MQ=255)
                                                                                  gCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGa        <  1:3121946/93‑1 (MQ=255)
                                                                                   cATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGAt       >  1:4276854/1‑93 (MQ=255)
                                                                                   cATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGAt       <  2:4718263/93‑1 (MQ=255)
                                                                                    atcacgatcacgTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGAtt      <  2:4916799/93‑1 (MQ=255)
                                                                                     tCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTa     >  2:292420/1‑93 (MQ=255)
                                                                                       aCGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATg   >  2:1487373/1‑93 (MQ=255)
                                                                                       aCGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATg   >  2:786324/1‑93 (MQ=255)
                                                                                        cGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATGc  >  2:5212901/1‑93 (MQ=255)
                                                                                        cGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATGc  >  2:3940568/1‑93 (MQ=255)
                                                                                           |                                                                                         
TTTTTCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATGC  >  NC_000913/3856236‑3856416

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTTTTCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATGCCAAT  >  NC_000913/3856235‑3856420
                                                                                            |                                                                                             
GTTTTTCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATC                                                                                              <  SRR4302537.3903645/94‑1 (MQ=60)
GTTTTTCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATC                                                                                              <  SRR4302537.4361273/94‑1 (MQ=60)
GTTTTTCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATC                                                                                              <  SRR4302537.1652511/94‑1 (MQ=60)
   TTTCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACG                                                                                           >  SRR4302537.5662770/1‑94 (MQ=60)
     TCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTT                                                                                         <  SRR4302537.5355420/94‑1 (MQ=60)
      ccgcggctgCATGTCCACAGCAAGCGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCGCCAGCATCACGATCACGTTG                                                                                        <  SRR4302537.4229465/85‑1 (MQ=60)
      CCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTG                                                                                        >  SRR4302537.5054144/1‑94 (MQ=60)
         CGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATG                                                                                     <  SRR4302537.5176574/94‑1 (MQ=60)
          GGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGC                                                                                    <  SRR4302537.2981021/94‑1 (MQ=60)
          GGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGC                                                                                    >  SRR4302537.3720180/1‑94 (MQ=60)
          GGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGC                                                                                    <  SRR4302537.764137/94‑1 (MQ=60)
           GTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCA                                                                                   <  SRR4302537.6395921/94‑1 (MQ=60)
              TCATGTCCACCACAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAA                                                                                >  SRR4302537.5044946/1‑94 (MQ=60)
                ATGTCCACCGCAAACGCATCTTTGAAGGGGAAAGGCGGGGCGCGAGGTTAGATAAAACCGATCACCAGCATCACGATCACGCTGATGCAGAACG                                                                              >  SRR4302537.4329751/1‑94 (MQ=60)
                 TGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGT                                                                             <  SRR4302537.304329/94‑1 (MQ=60)
                 TGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGA                                                                             >  SRR4302537.800155/1‑94 (MQ=60)
                  GTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTA                                                                            >  SRR4302537.2894265/1‑94 (MQ=60)
                   TCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTAC                                                                           <  SRR4302537.5757812/94‑1 (MQ=60)
                   TCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTAC                                                                           >  SRR4302537.6236155/1‑94 (MQ=60)
                   TCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTAC                                                                           <  SRR4302537.4790540/94‑1 (MQ=60)
                   TCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTAC                                                                           <  SRR4302537.2741710/94‑1 (MQ=60)
                     CACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCTCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAG                                                                         <  SRR4302537.54068/94‑1 (MQ=60)
                     CACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAG                                                                         >  SRR4302537.587049/1‑94 (MQ=60)
                     CACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAG                                                                         <  SRR4302537.940409/94‑1 (MQ=60)
                     CACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAG                                                                         >  SRR4302537.3721437/1‑94 (MQ=60)
                     CACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAG                                                                         <  SRR4302537.45000/94‑1 (MQ=60)
                     CACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAG                                                                         <  SRR4302537.5400640/94‑1 (MQ=60)
                     CACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACAAGCATCACGATCACGTTGATGCAGAACGTACAG                                                                         >  SRR4302537.716374/1‑94 (MQ=60)
                      ACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGG                                                                        >  SRR4302537.4919380/1‑94 (MQ=60)
                      ACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGG                                                                        >  SRR4302537.2253962/1‑94 (MQ=60)
                        CGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCC                                                                      >  SRR4302537.6168789/1‑94 (MQ=60)
                        CGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCC                                                                      <  SRR4302537.2890021/94‑1 (MQ=60)
                        CGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCC                                                                      <  SRR4302537.4013340/94‑1 (MQ=60)
                          CAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCCG                                                                    >  SRR4302537.1178289/1‑94 (MQ=60)
                          CAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAG                                                                    >  SRR4302537.5614379/1‑94 (MQ=60)
                            AACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCA                                                                  >  SRR4302537.1996442/1‑94 (MQ=60)
                             ACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCAC                                                                 <  SRR4302537.1254689/94‑1 (MQ=60)
                              CGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAGCCTATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACA                                                                <  SRR4302537.4989343/94‑1 (MQ=60)
                              CGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACA                                                                >  SRR4302537.4304087/1‑94 (MQ=60)
                               GCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACAT                                                               >  SRR4302537.6485472/1‑94 (MQ=60)
                               GCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACAT                                                               <  SRR4302537.3083003/94‑1 (MQ=60)
                               GCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACAT                                                               <  SRR4302537.6221706/94‑1 (MQ=60)
                               GCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACAT                                                               >  SRR4302537.6239564/1‑94 (MQ=60)
                                CATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATA                                                              <  SRR4302537.2468157/94‑1 (MQ=60)
                                 ATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAG                                                             >  SRR4302537.1716606/1‑94 (MQ=60)
                                 ATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAG                                                             >  SRR4302537.4929395/1‑94 (MQ=60)
                                 ATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAG                                                             <  SRR4302537.6477378/94‑1 (MQ=60)
                                  TCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGA                                                            >  SRR4302537.3208497/1‑94 (MQ=60)
                                  TCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGA                                                            <  SRR4302537.1132892/94‑1 (MQ=60)
                                  TCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGA                                                            <  SRR4302537.2053876/94‑1 (MQ=60)
                                   CTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAG                                                           <  SRR4302537.4858694/94‑1 (MQ=60)
                                   CTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAG                                                           >  SRR4302537.4440947/1‑94 (MQ=60)
                                   CTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAG                                                           <  SRR4302537.5281017/94‑1 (MQ=60)
                                    TTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGG                                                          <  SRR4302537.5310124/94‑1 (MQ=60)
                                     TTNAAGNTNAACGGCGTGGCGCNNGGTTTGATAAAACCGATCACCNGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGA                                                         <  SRR4302537.4723446/94‑1 (MQ=60)
                                         AGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATG                                                     >  SRR4302537.721908/1‑94 (MQ=60)
                                          GGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGG                                                    <  SRR4302537.2406185/94‑1 (MQ=60)
                                          GGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGG                                                    <  SRR4302537.176013/94‑1 (MQ=60)
                                            TGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACACGCCAGCACATAGAGGAAATGGAA                                                  >  SRR4302537.1616145/1‑94 (MQ=60)
                                             GAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAG                                                 >  SRR4302537.848651/1‑94 (MQ=60)
                                             GAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAG                                                 <  SRR4302537.5493958/94‑1 (MQ=60)
                                             GAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAG                                                 <  SRR4302537.1656834/94‑1 (MQ=60)
                                              AACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGT                                                >  SRR4302537.2983332/1‑94 (MQ=60)
                                               ACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTC                                               <  SRR4302537.3673893/94‑1 (MQ=60)
                                               ACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTC                                               <  SRR4302537.5813134/94‑1 (MQ=60)
                                                CGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCG                                              <  SRR4302537.4916941/94‑1 (MQ=60)
                                                 GGCGTGGCGCGCGGTTTGATAAAGCCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGA                                             <  SRR4302537.5740684/94‑1 (MQ=60)
                                                 GGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCGCGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGA                                             <  SRR4302537.2154699/94‑1 (MQ=60)
                                                 GGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGA                                             >  SRR4302537.6430029/1‑94 (MQ=60)
                                                 GGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGA                                             >  SRR4302537.4356492/1‑94 (MQ=60)
                                                  GCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACAGAGAGGAAATGGAAGTCGAA                                            <  SRR4302537.1766459/94‑1 (MQ=60)
                                                   CGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAAC                                           >  SRR4302537.1037006/1‑94 (MQ=60)
                                                   CGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAAC                                           <  SRR4302537.505836/94‑1 (MQ=60)
                                                   CGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAAC                                           >  SRR4302537.2126190/1‑94 (MQ=60)
                                                    GTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACT                                          >  SRR4302537.2755256/1‑94 (MQ=60)
                                                     TGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTT                                         >  SRR4302537.2597324/1‑94 (MQ=60)
                                                      GGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTC                                        <  SRR4302537.4723094/94‑1 (MQ=60)
                                                       GCGCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCA                                       <  SRR4302537.1872753/94‑1 (MQ=60)
                                                         GCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACC                                     <  SRR4302537.1750497/94‑1 (MQ=60)
                                                         GCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACC                                     >  SRR4302537.2069656/1‑94 (MQ=60)
                                                         GCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACC                                     <  SRR4302537.2243050/94‑1 (MQ=60)
                                                         GCGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACC                                     <  SRR4302537.3121760/94‑1 (MQ=60)
                                                          CGCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCA                                    <  SRR4302537.861603/94‑1 (MQ=60)
                                                           GCGGTTTGATTAAACCGATCACCAGCATCACGATCACGTTAATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAG                                   >  SRR4302537.2243125/1‑94 (MQ=60)
                                                           GCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAG                                   >  SRR4302537.6494517/1‑94 (MQ=60)
                                                           GCGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAG                                   >  SRR4302537.2199578/1‑94 (MQ=60)
                                                            CGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGA                                  <  SRR4302537.3382027/94‑1 (MQ=60)
                                                            CGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGA                                  >  SRR4302537.1549575/1‑94 (MQ=60)
                                                            CGGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGA                                  <  SRR4302537.3293310/94‑1 (MQ=60)
                                                            CGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGA                                  >  SRR4302537.6365589/1‑94 (MQ=60)
                                                             GGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGAT                                 >  SRR4302537.763773/1‑94 (MQ=60)
                                                             GGTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGAT                                 >  SRR4302537.3242292/1‑94 (MQ=60)
                                                              GTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATA                                <  SRR4302537.4561305/94‑1 (MQ=60)
                                                              GTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATA                                >  SRR4302537.1309243/1‑94 (MQ=60)
                                                              GTTTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATA                                >  SRR4302537.2068838/1‑94 (MQ=60)
                                                                TTGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGT                              >  SRR4302537.885549/1‑94 (MQ=60)
                                                                 TGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTT                             >  SRR4302537.2130455/1‑94 (MQ=60)
                                                                 TGATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTT                             >  SRR4302537.4324522/1‑94 (MQ=60)
                                                                 TGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTT                             <  SRR4302537.2500367/94‑1 (MQ=60)
                                                                  GATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTG                            <  SRR4302537.3390129/94‑1 (MQ=60)
                                                                  GATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTG                            >  SRR4302537.940921/1‑94 (MQ=60)
                                                                   ATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGA                           >  SRR4302537.5464751/1‑94 (MQ=60)
                                                                   ATAAAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGA                           >  SRR4302537.2226365/1‑94 (MQ=60)
                                                                      AAACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCTAACTTCACCAGATAGTTGATGG                        <  SRR4302537.3786339/94‑1 (MQ=60)
                                                                       AACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGT                       <  SRR4302537.4346001/94‑1 (MQ=60)
                                                                       AACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGT                       <  SRR4302537.1330542/94‑1 (MQ=60)
                                                                       AACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGT                       >  SRR4302537.5527256/1‑94 (MQ=60)
                                                                       AACCGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGG                       >  SRR4302537.3357706/1‑94 (MQ=60)
                                                                          CGATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGAT                    >  SRR4302537.1370543/1‑94 (MQ=60)
                                                                           GATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATG                   <  SRR4302537.5612759/94‑1 (MQ=60)
                                                                           GATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGGGATG                   >  SRR4302537.4446380/1‑94 (MQ=60)
                                                                            ATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGT                  >  SRR4302537.2381243/1‑94 (MQ=60)
                                                                            ATCACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGT                  >  SRR4302537.5463509/1‑94 (MQ=60)
                                                                              CACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAG                <  SRR4302537.1741727/94‑1 (MQ=60)
                                                                               ACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGC               >  SRR4302537.1979932/1‑94 (MQ=60)
                                                                               ACCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGC               >  SRR4302537.3875820/1‑94 (MQ=60)
                                                                                CCAGCATCACGATCACGTTGATGCAGATCGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCT              <  SRR4302537.2824229/94‑1 (MQ=60)
                                                                                CCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCT              >  SRR4302537.3658300/1‑94 (MQ=60)
                                                                                CCAGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCT              <  SRR4302537.2480867/94‑1 (MQ=60)
                                                                                  AGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGA            >  SRR4302537.5487835/1‑94 (MQ=60)
                                                                                  AGCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGA            >  SRR4302537.2860662/1‑94 (MQ=60)
                                                                                   GCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGAT           >  SRR4302537.4306574/1‑94 (MQ=60)
                                                                                   GCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGAT           <  SRR4302537.3143784/94‑1 (MQ=60)
                                                                                   GCATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGAT           <  SRR4302537.1374010/94‑1 (MQ=60)
                                                                                    CATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATT          >  SRR4302537.6123861/1‑94 (MQ=60)
                                                                                    CATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATT          >  SRR4302537.158079/1‑94 (MQ=60)
                                                                                    CATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATT          >  SRR4302537.3599674/1‑94 (MQ=60)
                                                                                     ATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTA         <  SRR4302537.4817133/94‑1 (MQ=60)
                                                                                     ATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTA         >  SRR4302537.5865162/1‑94 (MQ=60)
                                                                                     ATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTA         >  SRR4302537.2694988/1‑94 (MQ=60)
                                                                                     ATCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTA         <  SRR4302537.2438950/94‑1 (MQ=60)
                                                                                      TCACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTAT        <  SRR4302537.2346307/94‑1 (MQ=60)
                                                                                       CACGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATG       >  SRR4302537.5851828/1‑94 (MQ=60)
                                                                                         CGATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATGCC     <  SRR4302537.6341841/94‑1 (MQ=60)
                                                                                          GATCACGTTGATGCAGAACGTACATGCCAGCACATAGAGGAAATTGATGTCGAACTTCACCATATAGTTTATGGTGATGTAGCTGATTATGCCA    >  SRR4302537.6520487/1‑94 (MQ=60)
                                                                                          GATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATGCCA    >  SRR4302537.5161327/1‑94 (MQ=60)
                                                                                           ATCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATGCCAA   >  SRR4302537.5101824/1‑94 (MQ=60)
                                                                                            TCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATGCCAAT  <  SRR4302537.5450560/94‑1 (MQ=60)
                                                                                            TCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATCGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATGCCAAT  >  SRR4302537.3063782/1‑94 (MQ=60)
                                                                                            |                                                                                             
GTTTTTCCACGGTTTCATGTCCACCGCAAACGCATCTTTGAAGGTGAACGGCGTGGCGCGCGGTTTGATAAAACCGATCACCAGCATCACGACCACGTTGATGCAGAACGTACAGGCCAGCACATAGAGGAAATGGAAGTCGAACTTCACCAGATAGTTGATGGTGATGTAGCTGATTATGCCAAT  >  NC_000913/3856235‑3856420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑