Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,867,690 G→A T236T (ACG→ACA)  yidS → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,867,6900GA96.5% 1008.3 / 11.6 289T236T (ACG→ACA) yidShypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (3/7);  new base A (145/134);  total (148/141)
Fisher's exact test for biased strand distribution p-value = 2.09e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.71e-01

Only 100 of 299 total aligned reads displayed.

TAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCA  >  NC_000913/3867600‑3867779
                                                                                          |                                                                                         
taactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACt                                                                                         >  2:875419/1‑93 (MQ=255)
taactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACt                                                                                         >  2:1495816/1‑93 (MQ=255)
 aactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGccg                                                                                        <  1:6343699/93‑3 (MQ=255)
 aactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTg                                                                                        >  1:1251450/1‑93 (MQ=255)
 aactGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTg                                                                                        >  2:5683584/1‑93 (MQ=255)
  actGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGa                                                                                       <  2:1895944/93‑1 (MQ=255)
   ctGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGaa                                                                                      >  2:3016974/1‑93 (MQ=255)
    tGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGaaa                                                                                     <  2:6210103/93‑1 (MQ=255)
      ttATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAaga                                                                                   <  2:5648772/93‑1 (MQ=255)
        aTTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAgaga                                                                                 >  2:4245718/1‑93 (MQ=255)
        aTTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAgaga                                                                                 <  2:3493756/93‑1 (MQ=255)
          tCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGaaa                                                                               >  1:689169/1‑93 (MQ=255)
          tCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGaaa                                                                               <  1:2092371/93‑1 (MQ=255)
           cATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGaaaa                                                                              <  1:956412/93‑1 (MQ=255)
            aTGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAAt                                                                             <  2:5865807/93‑1 (MQ=255)
            aTGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAAt                                                                             <  2:2774365/93‑1 (MQ=255)
             tGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATg                                                                            >  1:5951626/1‑93 (MQ=255)
             tGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATg                                                                            <  2:5527034/93‑1 (MQ=255)
              ggAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGa                                                                           <  2:2085655/93‑1 (MQ=255)
                aGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAgc                                                                         >  1:3408599/1‑93 (MQ=255)
                aGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAgc                                                                         >  1:4533846/1‑93 (MQ=255)
                  tATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAgcgc                                                                       >  2:5008829/1‑93 (MQ=255)
                     cAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCtt                                                                    >  2:3759574/1‑93 (MQ=255)
                     cAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCtt                                                                    >  1:3310185/1‑93 (MQ=255)
                      aGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCttt                                                                   >  2:1179729/1‑93 (MQ=255)
                      aGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCttt                                                                   >  1:524575/1‑93 (MQ=255)
                        cAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCATTTCa                                                                 >  2:6382633/1‑93 (MQ=255)
                         aaggACGGTTATTTTATCTTGGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTCTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAg                                                                <  2:2374438/90‑1 (MQ=255)
                         aaaGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAg                                                                >  1:1404185/1‑93 (MQ=255)
                          aaGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGt                                                               >  2:1251365/1‑93 (MQ=255)
                          aaGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGt                                                               <  1:5052987/93‑1 (MQ=255)
                          aaGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGt                                                               >  2:3117659/1‑93 (MQ=255)
                          aaGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGt                                                               >  2:5987159/1‑93 (MQ=255)
                          aaGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCAAGAGACTGAAAGAGAAAATGCGCGCCTTTCAGt                                                               >  2:2204686/1‑93 (MQ=255)
                           aGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCCGAAAGAGAAAATGAGCGCCTTTCAGtt                                                              >  2:9517/1‑93 (MQ=255)
                           aGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGtt                                                              <  2:83123/93‑1 (MQ=255)
                           aGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGtt                                                              >  1:1837187/1‑93 (MQ=255)
                             aCGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTcc                                                            >  2:3503113/1‑93 (MQ=255)
                              cGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCa                                                           <  1:1768509/93‑1 (MQ=255)
                                gTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGt                                                         <  2:1812061/93‑1 (MQ=255)
                                 ttATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGtt                                                        <  1:1033566/93‑1 (MQ=255)
                                  tATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGttt                                                       <  1:5392669/93‑1 (MQ=255)
                                   aTTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTg                                                      <  1:2467321/93‑1 (MQ=255)
                                   aTTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTg                                                      >  1:1115001/1‑93 (MQ=255)
                                   aTTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTGTCAGTTCCAGTTTg                                                      <  2:344140/93‑1 (MQ=255)
                                    ttttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCCGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTgg                                                     <  1:4129040/93‑1 (MQ=255)
                                    ttttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTgg                                                     >  2:4670252/1‑93 (MQ=255)
                                     tttATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGt                                                    <  1:3770303/93‑1 (MQ=255)
                                       tATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTaa                                                  <  2:2556455/93‑1 (MQ=255)
                                        aTCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGGGCGCCTTTCAGTTCCAGTTTGGTAAg                                                 >  1:877867/1‑93 (MQ=255)
                                        aTCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAg                                                 <  2:1051204/93‑1 (MQ=255)
                                        aTCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAg                                                 >  2:3614308/1‑93 (MQ=255)
                                         tCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGa                                                >  1:3600061/1‑93 (MQ=255)
                                          cTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGAc                                               >  1:5718930/1‑93 (MQ=255)
                                           tttGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACg                                              <  1:2691238/93‑1 (MQ=255)
                                           tttGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACg                                              <  2:1146387/93‑1 (MQ=255)
                                           tttGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACg                                              <  1:1992487/93‑1 (MQ=255)
                                           tttGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGATTGGTAAGACg                                              >  2:4848762/1‑93 (MQ=255)
                                            ttGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACgg                                             <  2:3051868/93‑1 (MQ=255)
                                             tGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCCGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGt                                            <  1:4046305/93‑1 (MQ=255)
                                             tGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGt                                            <  2:709637/93‑1 (MQ=255)
                                               gCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGa                                          <  2:1422058/93‑1 (MQ=255)
                                                cGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaa                                         <  2:2687181/93‑1 (MQ=255)
                                                 ggTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaa                                        <  1:5656662/93‑1 (MQ=255)
                                                 ggTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaa                                        <  1:64204/93‑1 (MQ=255)
                                                  gTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                       <  2:5632392/93‑1 (MQ=255)
                                                  gTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                       >  2:5252481/1‑93 (MQ=255)
                                                  gTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                       <  1:4933618/93‑1 (MQ=255)
                                                  gTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGaaaa                                       >  1:314929/1‑93 (MQ=255)
                                                    gCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGc                                     <  2:2301580/93‑1 (MQ=255)
                                                      cTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGa                                   <  2:5405055/93‑1 (MQ=255)
                                                       tATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGaa                                  >  2:3209597/1‑93 (MQ=255)
                                                         tCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGaaaa                                >  1:5477647/1‑93 (MQ=255)
                                                           cAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAAt                              >  1:1187833/1‑93 (MQ=255)
                                                            aaTGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATg                             >  2:2883314/1‑93 (MQ=255)
                                                             aTGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGc                            >  2:632591/1‑93 (MQ=255)
                                                             aTGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGc                            >  2:3946763/1‑93 (MQ=255)
                                                              tGAAAGACGGTCAGACGCGTTTCACGACGCCGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCa                           <  1:3636105/93‑1 (MQ=255)
                                                              tGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCa                           <  1:6229112/93‑1 (MQ=255)
                                                              tGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCa                           <  1:3661131/93‑1 (MQ=255)
                                                              tGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCa                           >  1:1670517/1‑93 (MQ=255)
                                                               gAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCAc                          >  1:4175910/1‑93 (MQ=255)
                                                               gAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCAc                          <  1:3032781/93‑1 (MQ=255)
                                                                aaaGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTCAGACGGTGAAAAGCGAAAAATGCACg                         >  1:6146316/1‑93 (MQ=255)
                                                                aaaGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACg                         <  1:2537237/93‑1 (MQ=255)
                                                                aaaGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACg                         <  2:368591/93‑1 (MQ=255)
                                                                  aGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGt                       >  2:6307163/1‑93 (MQ=255)
                                                                    aCGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGc                     >  2:680394/1‑93 (MQ=255)
                                                                         cAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGttt                <  1:2959109/93‑1 (MQ=255)
                                                                          aGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCAAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTc               <  1:4885046/93‑1 (MQ=255)
                                                                           gACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTcc              <  2:1728461/93‑1 (MQ=255)
                                                                              gcgTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTc           <  2:4541063/93‑1 (MQ=255)
                                                                              gcgTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTc           <  1:1071567/93‑1 (MQ=255)
                                                                               cgTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTcg          >  2:4072923/1‑93 (MQ=255)
                                                                                gTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTcgc         <  1:552719/93‑1 (MQ=255)
                                                                                  ttCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCgcgc       >  2:5091863/1‑93 (MQ=255)
                                                                                  ttCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCgcgc       <  1:3969495/93‑1 (MQ=255)
                                                                                   tCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCt      <  1:1488085/93‑1 (MQ=255)
                                                                                     acgacaCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTgg    <  1:5753707/93‑1 (MQ=255)
                                                                                       gacaCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGAACGGTGCTGTTTCCCTCGCGCTGGCa  >  1:1466319/1‑93 (MQ=255)
                                                                                          |                                                                                         
TAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCA  >  NC_000913/3867600‑3867779

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAT  >  NC_000913/3867597‑3867783
                                                                                             |                                                                                             
AACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACA                                                                                               <  SRR4302537.4906405/94‑1 (MQ=60)
AACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACA                                                                                               >  SRR4302537.6296248/1‑94 (MQ=60)
AACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACA                                                                                               >  SRR4302537.6386453/1‑94 (MQ=60)
 ACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACAC                                                                                              <  SRR4302537.4221328/94‑1 (MQ=60)
 ACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACAC                                                                                              >  SRR4302537.3860364/1‑94 (MQ=60)
 ACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACAC                                                                                              <  SRR4302537.2120036/94‑1 (MQ=60)
  CTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACT                                                                                             >  SRR4302537.2659323/1‑94 (MQ=60)
   TAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCCG                                                                                            <  SRR4302537.67570/94‑1 (MQ=60)
   TAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTG                                                                                            <  SRR4302537.1966754/94‑1 (MQ=60)
   TAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTG                                                                                            >  SRR4302537.1260161/1‑94 (MQ=60)
    AACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCCGA                                                                                           <  SRR4302537.6387033/94‑1 (MQ=60)
    AACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGA                                                                                           >  SRR4302537.3684834/1‑94 (MQ=60)
    AACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGA                                                                                           >  SRR4302537.2554405/1‑94 (MQ=60)
     ACTGTTATTCATGGAGTATCAGCAAAGACTGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAA                                                                                          <  SRR4302537.1792687/94‑1 (MQ=60)
     ACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAA                                                                                          <  SRR4302537.3204652/94‑1 (MQ=60)
      CTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAA                                                                                         >  SRR4302537.5225472/1‑94 (MQ=60)
       TGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAG                                                                                        <  SRR4302537.1348318/94‑1 (MQ=60)
        GTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGA                                                                                       >  SRR4302537.2099309/1‑94 (MQ=60)
         TTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAG                                                                                      <  SRR4302537.974720/94‑1 (MQ=60)
         TTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAG                                                                                      <  SRR4302537.1416165/94‑1 (MQ=60)
           ATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAA                                                                                    >  SRR4302537.632058/1‑94 (MQ=60)
            TTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAA                                                                                   >  SRR4302537.693888/1‑94 (MQ=60)
            TTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAA                                                                                   >  SRR4302537.3385499/1‑94 (MQ=60)
             TCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAA                                                                                  >  SRR4302537.5595562/1‑94 (MQ=60)
             TCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAA                                                                                  <  SRR4302537.2107128/94‑1 (MQ=60)
              CATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAAT                                                                                 <  SRR4302537.963009/94‑1 (MQ=60)
              CATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAAT                                                                                 >  SRR4302537.1113445/1‑94 (MQ=60)
              CATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAAT                                                                                 <  SRR4302537.360376/94‑1 (MQ=60)
              CATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAAT                                                                                 >  SRR4302537.186626/1‑94 (MQ=60)
               atagattatcagcaaattttttttttttatcgggGGCGGTGCCTATCAAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATG                                                                                <  SRR4302537.4032858/60‑1 (MQ=60)
               ATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATG                                                                                >  SRR4302537.5992483/1‑94 (MQ=60)
                TGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGA                                                                               >  SRR4302537.1489643/1‑94 (MQ=60)
                TGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGA                                                                               <  SRR4302537.1226789/94‑1 (MQ=60)
                TGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGA                                                                               <  SRR4302537.39999/94‑1 (MQ=60)
                TGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGA                                                                               <  SRR4302537.5758985/94‑1 (MQ=60)
                  GAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGC                                                                             >  SRR4302537.6145679/1‑94 (MQ=60)
                  GAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGC                                                                             >  SRR4302537.4565237/1‑94 (MQ=60)
                  GAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGC                                                                             >  SRR4302537.3432748/1‑94 (MQ=60)
                  GAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGC                                                                             <  SRR4302537.5062142/94‑1 (MQ=60)
                  GAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGC                                                                             >  SRR4302537.5564084/1‑94 (MQ=60)
                     TATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCC                                                                          >  SRR4302537.3008654/1‑94 (MQ=60)
                      ATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCT                                                                         <  SRR4302537.1776716/94‑1 (MQ=60)
                      ATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCT                                                                         <  SRR4302537.556821/94‑1 (MQ=60)
                       TCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCCGAAAGAGAAAATGAGCGCCTT                                                                        <  SRR4302537.1076247/94‑1 (MQ=60)
                       TCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTT                                                                        >  SRR4302537.3333601/1‑94 (MQ=60)
                        CAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTT                                                                       >  SRR4302537.3155828/1‑94 (MQ=60)
                        CAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTT                                                                       >  SRR4302537.528174/1‑94 (MQ=60)
                         AGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTC                                                                      <  SRR4302537.4136375/94‑1 (MQ=60)
                          GCAAAGTCGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCA                                                                     <  SRR4302537.1827605/94‑1 (MQ=60)
                          GCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCCGAAAGAGAAAATGAGCGCCTTTCA                                                                     >  SRR4302537.6179777/1‑94 (MQ=60)
                           CAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAG                                                                    <  SRR4302537.3783937/94‑1 (MQ=60)
                           CAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAG                                                                    <  SRR4302537.6388180/94‑1 (MQ=60)
                           CAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAG                                                                    >  SRR4302537.1413964/1‑94 (MQ=60)
                           CAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAG                                                                    >  SRR4302537.515629/1‑94 (MQ=60)
                             AAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTT                                                                  >  SRR4302537.1849976/1‑94 (MQ=60)
                             AAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTT                                                                  <  SRR4302537.5087776/94‑1 (MQ=60)
                              AGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTC                                                                 >  SRR4302537.266832/1‑94 (MQ=60)
                                 CGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAG                                                              <  SRR4302537.1212475/94‑1 (MQ=60)
                                 CGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAG                                                              <  SRR4302537.1780821/94‑1 (MQ=60)
                                  GGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGT                                                             >  SRR4302537.5783759/1‑94 (MQ=60)
                                  GGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGT                                                             >  SRR4302537.1182005/1‑94 (MQ=60)
                                   GTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTT                                                            <  SRR4302537.944001/94‑1 (MQ=60)
                                    TTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTT                                                           <  SRR4302537.1040687/94‑1 (MQ=60)
                                    TTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTT                                                           <  SRR4302537.372447/94‑1 (MQ=60)
                                     TATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTG                                                          <  SRR4302537.5429503/94‑1 (MQ=60)
                                     TATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTG                                                          >  SRR4302537.1122696/1‑94 (MQ=60)
                                     TATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGGCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTACAGTTTG                                                          >  SRR4302537.3034714/1‑94 (MQ=60)
                                      ATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGG                                                         <  SRR4302537.3194758/94‑1 (MQ=60)
                                      ATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGG                                                         <  SRR4302537.2484667/94‑1 (MQ=60)
                                      ATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGG                                                         >  SRR4302537.3916891/1‑94 (MQ=60)
                                      ATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGG                                                         <  SRR4302537.2276786/94‑1 (MQ=60)
                                       TTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACTCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGT                                                        <  SRR4302537.620241/94‑1 (MQ=60)
                                       TTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCCGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGT                                                        <  SRR4302537.4157858/94‑1 (MQ=60)
                                        TTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTA                                                       >  SRR4302537.3638192/1‑94 (MQ=60)
                                        TTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTA                                                       >  SRR4302537.570037/1‑94 (MQ=60)
                                        TTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTA                                                       <  SRR4302537.3416792/94‑1 (MQ=60)
                                        TTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTA                                                       <  SRR4302537.3796772/94‑1 (MQ=60)
                                         TTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAA                                                      >  SRR4302537.4153950/1‑94 (MQ=60)
                                          TATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGGGCGCCTTTCAGTTCCAGTTTGGTAAG                                                     >  SRR4302537.883905/1‑94 (MQ=60)
                                           ATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGA                                                    >  SRR4302537.4961757/1‑94 (MQ=60)
                                           ATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGA                                                    >  SRR4302537.3625435/1‑94 (MQ=60)
                                            TCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGAC                                                   >  SRR4302537.5758072/1‑94 (MQ=60)
                                            TCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGAC                                                   <  SRR4302537.4867519/94‑1 (MQ=60)
                                             CTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACG                                                  >  SRR4302537.3843410/1‑94 (MQ=60)
                                             CTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACG                                                  >  SRR4302537.740178/1‑94 (MQ=60)
                                              TTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGG                                                 <  SRR4302537.5892914/94‑1 (MQ=60)
                                              TTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGG                                                 <  SRR4302537.2710019/94‑1 (MQ=60)
                                              TTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGG                                                 <  SRR4302537.4224075/94‑1 (MQ=60)
                                              TTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGG                                                 <  SRR4302537.2006343/94‑1 (MQ=60)
                                               TTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGT                                                <  SRR4302537.5820671/94‑1 (MQ=60)
                                                TGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCCGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTG                                               <  SRR4302537.4074598/94‑1 (MQ=60)
                                                    GGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAA                                           >  SRR4302537.4191285/1‑94 (MQ=60)
                                                    GGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAA                                           >  SRR4302537.317110/1‑94 (MQ=60)
                                                    GGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAA                                           <  SRR4302537.64682/94‑1 (MQ=60)
                                                    GGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAA                                           <  SRR4302537.5695369/94‑1 (MQ=60)
                                                    GGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAA                                           >  SRR4302537.6450948/1‑94 (MQ=60)
                                                     GTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAG                                          <  SRR4302537.4967647/94‑1 (MQ=60)
                                                      TGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGC                                         >  SRR4302537.1623721/1‑94 (MQ=60)
                                                       GCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCG                                        <  SRR4302537.4983949/94‑1 (MQ=60)
                                                       GCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCG                                        <  SRR4302537.151442/94‑1 (MQ=60)
                                                        CCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGA                                       >  SRR4302537.4575989/1‑94 (MQ=60)
                                                         CTATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAA                                      >  SRR4302537.2092137/1‑94 (MQ=60)
                                                          TATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAA                                     <  SRR4302537.1173508/94‑1 (MQ=60)
                                                          TATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAA                                     <  SRR4302537.6474272/94‑1 (MQ=60)
                                                          TATACAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAA                                     >  SRR4302537.2821415/1‑94 (MQ=60)
                                                           ATCCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAA                                    >  SRR4302537.5515039/1‑94 (MQ=60)
                                                             CCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAAT                                  >  SRR4302537.1196086/1‑94 (MQ=60)
                                                             CCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAAT                                  <  SRR4302537.3660730/94‑1 (MQ=60)
                                                             CCAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAAT                                  <  SRR4302537.1471464/94‑1 (MQ=60)
                                                              CAATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATG                                 >  SRR4302537.1710344/1‑94 (MQ=60)
                                                                ATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCA                               >  SRR4302537.6364871/1‑94 (MQ=60)
                                                                ATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCA                               >  SRR4302537.1682218/1‑94 (MQ=60)
                                                                ATGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCA                               >  SRR4302537.2334433/1‑94 (MQ=60)
                                                                 TGAAAGACGGTCAGACGCGTTTCACGACGCCGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCAC                              <  SRR4302537.3661714/94‑1 (MQ=60)
                                                                 TGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCAC                              <  SRR4302537.6271964/94‑1 (MQ=60)
                                                                 TGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCAC                              >  SRR4302537.4205064/1‑94 (MQ=60)
                                                                 TGAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCAC                              <  SRR4302537.3686900/94‑1 (MQ=60)
                                                                  GAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTCAGACGGTGAAAAGCGAAAAATGCACG                             >  SRR4302537.6188590/1‑94 (MQ=60)
                                                                  GAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACG                             <  SRR4302537.6231316/94‑1 (MQ=60)
                                                                  GAAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACG                             <  SRR4302537.3053927/94‑1 (MQ=60)
                                                                   AAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAGAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGG                            <  SRR4302537.3563332/94‑1 (MQ=60)
                                                                   AAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGG                            >  SRR4302537.2883132/1‑94 (MQ=60)
                                                                   AAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGG                            >  SRR4302537.921075/1‑94 (MQ=60)
                                                                   AAAGACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGG                            <  SRR4302537.2555038/94‑1 (MQ=60)
                                                                    AAGACGGTCAGACGCGTTTCACGACGCCGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGT                           <  SRR4302537.70724/94‑1 (MQ=60)
                                                                      GACGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGC                         <  SRR4302537.5296084/94‑1 (MQ=60)
                                                                        CGGTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTG                       >  SRR4302537.1102138/1‑94 (MQ=60)
                                                                          GTCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTT                     <  SRR4302537.5532297/94‑1 (MQ=60)
                                                                           TCAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACCGTGAAAAGCGAAAAATGCACGcacctgttt                    >  SRR4302537.3158119/1‑85 (MQ=60)
                                                                            CAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTC                   >  SRR4302537.4047036/1‑94 (MQ=60)
                                                                            CAGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTC                   <  SRR4302537.2979872/94‑1 (MQ=60)
                                                                             AGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCC                  >  SRR4302537.5710650/1‑94 (MQ=60)
                                                                             AGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCC                  <  SRR4302537.4934773/94‑1 (MQ=60)
                                                                             AGACGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCAAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCC                  <  SRR4302537.4918780/94‑1 (MQ=60)
                                                                                CGCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTC               <  SRR4302537.4500868/94‑1 (MQ=60)
                                                                                 GCGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCG              <  SRR4302537.1078954/94‑1 (MQ=60)
                                                                                  CGTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGC             >  SRR4302537.1480906/1‑94 (MQ=60)
                                                                                   GTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCG            >  SRR4302537.2525998/1‑94 (MQ=60)
                                                                                   GTTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCG            <  SRR4302537.556522/94‑1 (MQ=60)
                                                                                    TTTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGC           >  SRR4302537.3666525/1‑94 (MQ=60)
                                                                                     TTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCT          >  SRR4302537.5444167/1‑94 (MQ=60)
                                                                                     TTCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCT          <  SRR4302537.3997273/94‑1 (MQ=60)
                                                                                      TCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTG         <  SRR4302537.1498438/94‑1 (MQ=60)
                                                                                      TCACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTG         <  SRR4302537.1279494/94‑1 (MQ=60)
                                                                                       CACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGG        <  SRR4302537.5430694/94‑1 (MQ=60)
                                                                                        ACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGC       >  SRR4302537.4202970/1‑94 (MQ=60)
                                                                                        ACGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGC       <  SRR4302537.5793118/94‑1 (MQ=60)
                                                                                         CGACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGAACGGTGCTGTTTCCCTCGCGCTGGCA      >  SRR4302537.1476519/1‑94 (MQ=60)
                                                                                          GACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAG     <  SRR4302537.5342961/94‑1 (MQ=60)
                                                                                           ACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGG    >  SRR4302537.1119763/1‑94 (MQ=60)
                                                                                           ACACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGG    <  SRR4302537.3905790/94‑1 (MQ=60)
                                                                                             ACTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAT  >  SRR4302537.4046819/1‑94 (MQ=60)
                                                                                             |                                                                                             
AACTAACTGTTATTCATGGAGTATCAGCAAAGACGGTTATTTTATCTTTGGCGGTGCCTATCCAATGAAAGACGGTCAGACGCGTTTCACGACGCTGAAAGAGAAAATGAGCGCCTTTCAGTTCCAGTTTGGTAAGACGGTGAAAAGCGAAAAATGCACGGTGCTGTTTCCCTCGCGCTGGCAGGAT  >  NC_000913/3867597‑3867783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑