Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,899,143 G→A P236S (CCG→TCG)  yieC ← putative receptor protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,899,1430GA97.5% 852.5 / 2.7 239P236S (CCG→TCG) yieCputative receptor protein
Reads supporting (aligned to +/- strand):  ref base G (3/3);  new base A (104/129);  total (107/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.83e-01

Only 100 of 249 total aligned reads displayed.

CCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATAT  >  NC_000913/3899052‑3899232
                                                                                           |                                                                                         
   ggACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCa                                                                                       >  1:5169298/1‑93 (MQ=255)
     aCCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCAcc                                                                                     >  2:3844902/1‑93 (MQ=255)
     aCCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCAcc                                                                                     <  2:5305833/93‑1 (MQ=255)
      ccGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCg                                                                                    >  1:4795407/1‑93 (MQ=255)
       cGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCgt                                                                                   <  1:2980746/93‑1 (MQ=255)
        gACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCgtg                                                                                  >  2:1858915/1‑93 (MQ=255)
        gACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCgtg                                                                                  >  2:1526188/1‑93 (MQ=255)
         aCTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCgtgt                                                                                 >  1:1122615/1‑93 (MQ=255)
          cTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGtt                                                                                <  2:4575636/93‑1 (MQ=255)
          cTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGtt                                                                                <  2:6007472/93‑1 (MQ=255)
           tttCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGttt                                                                               >  1:300425/1‑93 (MQ=255)
            ttCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTa                                                                              <  2:4007500/93‑1 (MQ=255)
            ttCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTa                                                                              <  2:5758824/93‑1 (MQ=255)
              ccAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTacc                                                                            <  2:4418232/93‑1 (MQ=255)
              ccAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTacc                                                                            >  1:2948155/1‑93 (MQ=255)
                aGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTaccca                                                                          >  2:1933041/1‑93 (MQ=255)
                 gTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTacccac                                                                         <  1:5718824/93‑1 (MQ=255)
                   tttCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTacccaccc                                                                       <  1:667405/93‑1 (MQ=255)
                      ccAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGa                                                                    >  2:3712804/1‑93 (MQ=255)
                         gACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAAt                                                                 >  2:1728960/1‑93 (MQ=255)
                          aCCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATc                                                                <  1:3335552/93‑1 (MQ=255)
                           ccTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCa                                                               <  2:4940687/93‑1 (MQ=255)
                           ccTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCa                                                               <  1:2286125/93‑1 (MQ=255)
                            cTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAg                                                              >  1:4829840/1‑93 (MQ=255)
                             tACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGc                                                             >  1:6043885/1‑93 (MQ=255)
                                ccccGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCAAGAAATCAGCctc                                                          <  2:2599439/92‑1 (MQ=255)
                                acCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCctc                                                          <  1:5577945/93‑1 (MQ=255)
                                 cccGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCctct                                                         >  2:5819494/1‑93 (MQ=255)
                                  ccGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTg                                                        <  2:4311570/93‑1 (MQ=255)
                                    gCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGgc                                                      <  2:2382867/93‑1 (MQ=255)
                                    gCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGgc                                                      >  1:6278656/1‑93 (MQ=255)
                                    gCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGgc                                                      >  1:2697458/1‑93 (MQ=255)
                                     cTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGgcg                                                     >  2:267395/1‑93 (MQ=255)
                                      tGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGgcgc                                                    >  1:2321962/1‑93 (MQ=255)
                                       gCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTCCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCa                                                   <  1:2911100/93‑1 (MQ=255)
                                           cATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGTGCAAAgg                                               >  1:1973190/1‑93 (MQ=255)
                                           cATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAgg                                               >  2:4245595/1‑93 (MQ=255)
                                           cATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAgg                                               <  1:2535644/93‑1 (MQ=255)
                                            aTCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAggg                                              >  1:4580619/1‑93 (MQ=255)
                                             tCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGc                                             <  2:1328982/93‑1 (MQ=255)
                                             tCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGc                                             <  1:3884188/93‑1 (MQ=255)
                                              cAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCa                                            <  1:3969791/93‑1 (MQ=255)
                                               aGTGCGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCCCCCAGAAATCAGCCTCTGGCGCAAAGGGCAg                                           <  1:2018837/93‑1 (MQ=255)
                                               aGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAg                                           >  2:4273462/1‑93 (MQ=255)
                                                gTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACAGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAgg                                          >  1:3702690/1‑93 (MQ=255)
                                                  aCGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGaa                                        >  2:5556352/1‑93 (MQ=255)
                                                   cGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGaaa                                       <  1:3588068/93‑1 (MQ=255)
                                                    gCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAAc                                      <  1:427007/93‑1 (MQ=255)
                                                     cTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCTCCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAAcc                                     >  1:5829757/1‑93 (MQ=255)
                                                     cTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAAcc                                     <  1:4350629/93‑1 (MQ=255)
                                                     cTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAAcc                                     <  1:4445950/93‑1 (MQ=255)
                                                      tGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCt                                    <  1:930837/93‑1 (MQ=255)
                                                       gCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCtt                                   <  1:4099194/93‑1 (MQ=255)
                                                       gCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCtt                                   >  1:1923903/1‑93 (MQ=255)
                                                        cGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCttt                                  <  2:2858927/93‑1 (MQ=255)
                                                        cGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCttt                                  <  1:4173980/93‑1 (MQ=255)
                                                        cGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCttt                                  <  2:1384290/93‑1 (MQ=255)
                                                         gTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTtg                                 <  2:1214594/93‑1 (MQ=255)
                                                         gTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTtg                                 <  1:5054064/93‑1 (MQ=255)
                                                          ttttCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTtgg                                >  1:5781077/1‑93 (MQ=255)
                                                          ttttCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTtgg                                <  2:3376479/93‑1 (MQ=255)
                                                              ccAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTggtggt                            >  1:2194231/1‑93 (MQ=255)
                                                                aGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTaa                          <  2:3078697/93‑1 (MQ=255)
                                                                 gTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAAc                         <  1:1222504/93‑1 (MQ=255)
                                                                 gTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAAc                         <  1:1658026/93‑1 (MQ=255)
                                                                  tCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACg                        >  1:3171895/1‑93 (MQ=255)
                                                                   cAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGt                       <  2:5031648/93‑1 (MQ=255)
                                                                    aaGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTa                      >  2:1608286/1‑93 (MQ=255)
                                                                    aaGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTa                      >  2:5933970/1‑93 (MQ=255)
                                                                     aGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTAc                     <  2:3315000/93‑1 (MQ=255)
                                                                      gCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACa                    <  2:2239252/93‑1 (MQ=255)
                                                                      gCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACa                    <  1:805567/93‑1 (MQ=255)
                                                                      gCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACa                    <  1:2603095/93‑1 (MQ=255)
                                                                      gCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACa                    <  2:5143736/93‑1 (MQ=255)
                                                                      gCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACa                    >  2:3641423/1‑93 (MQ=255)
                                                                       cATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACat                   >  2:841854/1‑93 (MQ=255)
                                                                         tCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACatat                 <  1:69927/93‑1 (MQ=255)
                                                                          cTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATc                <  2:3190050/93‑1 (MQ=255)
                                                                          cTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATc                <  2:1450233/93‑1 (MQ=255)
                                                                          cTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATc                <  2:1166691/93‑1 (MQ=255)
                                                                            ggATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCgg              <  2:2330690/93‑1 (MQ=255)
                                                                            ggATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCgg              >  1:2853747/1‑93 (MQ=255)
                                                                              aTTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGgag            <  1:1084070/93‑1 (MQ=255)
                                                                               tttCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGgaga           <  1:5283221/93‑1 (MQ=255)
                                                                               tttCAATTTTCGACGCACCGTGGTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACCTATCGgaga           >  2:2180469/1‑93 (MQ=255)
                                                                                ttCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGaa          >  2:6317180/1‑93 (MQ=255)
                                                                                ttCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGaa          <  1:2811837/93‑1 (MQ=255)
                                                                                 tCAATTTTCGACGCACCGTGTTTACCCACCCAGAAGTCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTCCATATCGGAGAAc         <  2:6207908/93‑1 (MQ=255)
                                                                                 tCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAAc         >  1:5356994/1‑93 (MQ=255)
                                                                                 tCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAAc         >  1:2512135/1‑93 (MQ=255)
                                                                                  cAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACt        <  2:766965/93‑1 (MQ=255)
                                                                                    aTTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGc      >  1:888331/1‑93 (MQ=255)
                                                                                    aTTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGc      <  2:4501071/93‑1 (MQ=255)
                                                                                    aTTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGc      <  2:4999577/93‑1 (MQ=255)
                                                                                     ttttCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCa     >  2:5127716/1‑93 (MQ=255)
                                                                                     ttttCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCa     >  1:5454996/1‑93 (MQ=255)
                                                                                      tttCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCat    >  1:1759112/1‑93 (MQ=255)
                                                                                      tttCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCat    <  2:4818882/93‑1 (MQ=255)
                                                                                       ttCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCata   >  1:5023143/1‑93 (MQ=255)
                                                                                       ttCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCata   <  2:292160/93‑1 (MQ=255)
                                                                                           |                                                                                         
CCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATAT  >  NC_000913/3899052‑3899232

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTT  >  NC_000913/3899051‑3899236
                                                                                            |                                                                                             
TCCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCGGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGAC                                                                                              <  SRR4302537.958104/94‑1 (MQ=60)
 CCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCG                                                                                             <  SRR4302537.83219/94‑1 (MQ=60)
 CCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACG                                                                                             >  SRR4302537.2955520/1‑94 (MQ=60)
 CCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACG                                                                                             >  SRR4302537.5254670/1‑94 (MQ=60)
 CCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACG                                                                                             <  SRR4302537.4137522/94‑1 (MQ=60)
 CCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACG                                                                                             <  SRR4302537.3254786/94‑1 (MQ=60)
   CGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCA                                                                                           >  SRR4302537.495414/1‑94 (MQ=60)
   CGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCA                                                                                           >  SRR4302537.5480774/1‑94 (MQ=60)
   CGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCA                                                                                           >  SRR4302537.5204899/1‑94 (MQ=60)
    GGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCAC                                                                                          <  SRR4302537.2578142/94‑1 (MQ=60)
      ACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCG                                                                                        >  SRR4302537.4828499/1‑94 (MQ=60)
      ACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCG                                                                                        <  SRR4302537.3382373/94‑1 (MQ=60)
       CCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGT                                                                                       >  SRR4302537.4862808/1‑94 (MQ=60)
        CGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTG                                                                                      <  SRR4302537.5368974/94‑1 (MQ=60)
        CGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTG                                                                                      <  SRR4302537.3048598/94‑1 (MQ=60)
        CGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTG                                                                                      <  SRR4302537.3001595/94‑1 (MQ=60)
         GACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGT                                                                                     >  SRR4302537.1130399/1‑94 (MQ=60)
         GACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGT                                                                                     >  SRR4302537.581955/1‑94 (MQ=60)
          ACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTT                                                                                    >  SRR4302537.642836/1‑94 (MQ=60)
           CTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTT                                                                                   >  SRR4302537.3723196/1‑94 (MQ=60)
           CTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTT                                                                                   >  SRR4302537.302520/1‑94 (MQ=60)
             TTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTAC                                                                                 >  SRR4302537.3970503/1‑94 (MQ=60)
              TCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACC                                                                                >  SRR4302537.2968847/1‑94 (MQ=60)
              TCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACC                                                                                <  SRR4302537.3001334/94‑1 (MQ=60)
                 AGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCAC                                                                             >  SRR4302537.1337703/1‑94 (MQ=60)
                  GTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTTTACGCACCGTGTTTACCCACC                                                                            >  SRR4302537.183191/1‑94 (MQ=60)
                  GTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACC                                                                            >  SRR4302537.2772595/1‑94 (MQ=60)
                  GTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACC                                                                            <  SRR4302537.5757966/94‑1 (MQ=60)
                    TTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTCCCCACCCA                                                                          <  SRR4302537.299126/94‑1 (MQ=60)
                    TTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCA                                                                          <  SRR4302537.671966/94‑1 (MQ=60)
                      TCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGA                                                                        >  SRR4302537.4731299/1‑94 (MQ=60)
                      TCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGA                                                                        >  SRR4302537.6292761/1‑94 (MQ=60)
                       CCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAA                                                                       >  SRR4302537.558886/1‑94 (MQ=60)
                       CCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAA                                                                       <  SRR4302537.1176629/94‑1 (MQ=60)
                        CAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAA                                                                      >  SRR4302537.1756517/1‑94 (MQ=60)
                           ACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCA                                                                   <  SRR4302537.3359158/94‑1 (MQ=60)
                           ACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCA                                                                   <  SRR4302537.3409292/94‑1 (MQ=60)
                            CCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAG                                                                  <  SRR4302537.2302250/94‑1 (MQ=60)
                            CCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAG                                                                  >  SRR4302537.4863199/1‑94 (MQ=60)
                            CCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAG                                                                  >  SRR4302537.3793308/1‑94 (MQ=60)
                             CTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGC                                                                 >  SRR4302537.6085398/1‑94 (MQ=60)
                             CTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGC                                                                 <  SRR4302537.5388190/94‑1 (MQ=60)
                               ACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCT                                                               >  SRR4302537.2765468/1‑94 (MQ=60)
                               ACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCT                                                               <  SRR4302537.691258/94‑1 (MQ=60)
                                 ACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCT                                                             <  SRR4302537.5616070/94‑1 (MQ=60)
                                  CCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTG                                                            <  SRR4302537.2271834/94‑1 (MQ=60)
                                   CCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCAACGTGTTTACCCACCCAGAAATCAGCCTCTGG                                                           >  SRR4302537.5983964/1‑94 (MQ=60)
                                    CGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGC                                                          <  SRR4302537.4016977/94‑1 (MQ=60)
                                    CGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGC                                                          >  SRR4302537.5824406/1‑94 (MQ=60)
                                    CGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGC                                                          >  SRR4302537.2857627/1‑94 (MQ=60)
                                    CGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGC                                                          >  SRR4302537.2716301/1‑94 (MQ=60)
                                    CGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGC                                                          >  SRR4302537.6321722/1‑94 (MQ=60)
                                      CTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGC                                                        >  SRR4302537.4258670/1‑94 (MQ=60)
                                      CTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGC                                                        <  SRR4302537.5083349/94‑1 (MQ=60)
                                      CTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGC                                                        >  SRR4302537.2338358/1‑94 (MQ=60)
                                       TGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCA                                                       >  SRR4302537.2611642/1‑94 (MQ=60)
                                        GCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTCCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAA                                                      <  SRR4302537.2931552/94‑1 (MQ=60)
                                          GGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAG                                                    <  SRR4302537.4147299/94‑1 (MQ=60)
                                          GGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAG                                                    <  SRR4302537.2913376/94‑1 (MQ=60)
                                           GCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGTGCAAAGG                                                   >  SRR4302537.1986906/1‑94 (MQ=60)
                                           GCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGG                                                   <  SRR4302537.4207277/94‑1 (MQ=60)
                                            CATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGG                                                  >  SRR4302537.4612306/1‑94 (MQ=60)
                                            CATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGG                                                  <  SRR4302537.2553436/94‑1 (MQ=60)
                                            CATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTACGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGG                                                  <  SRR4302537.2237788/94‑1 (MQ=60)
                                             ATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGC                                                 <  SRR4302537.312849/94‑1 (MQ=60)
                                              TCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCA                                                <  SRR4302537.3911356/94‑1 (MQ=60)
                                              TCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCA                                                <  SRR4302537.4406120/94‑1 (MQ=60)
                                              TCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCA                                                <  SRR4302537.2881502/94‑1 (MQ=60)
                                              TCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCA                                                <  SRR4302537.1295567/94‑1 (MQ=60)
                                               CAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAG                                               <  SRR4302537.3997571/94‑1 (MQ=60)
                                                AGTGCGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCCCCCAGAAATCAGCCTCTGGCGCAAAGGGCAGG                                              <  SRR4302537.2032915/94‑1 (MQ=60)
                                                AGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGG                                              <  SRR4302537.911844/94‑1 (MQ=60)
                                                AGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGG                                              <  SRR4302537.2488774/94‑1 (MQ=60)
                                                AGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACAGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGG                                              >  SRR4302537.3728734/1‑94 (MQ=60)
                                                    CGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAAC                                          <  SRR4302537.3613363/94‑1 (MQ=60)
                                                    CGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCAAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAAC                                          <  SRR4302537.5553433/94‑1 (MQ=60)
                                                     GCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCTCCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACC                                         >  SRR4302537.5869716/1‑94 (MQ=60)
                                                     GCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACC                                         <  SRR4302537.429975/94‑1 (MQ=60)
                                                     GCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACC                                         >  SRR4302537.712782/1‑94 (MQ=60)
                                                      CTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCT                                        <  SRR4302537.4380769/94‑1 (MQ=60)
                                                      CTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCT                                        <  SRR4302537.4476656/94‑1 (MQ=60)
                                                      CTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCT                                        >  SRR4302537.4697015/1‑94 (MQ=60)
                                                       TGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTT                                       <  SRR4302537.4965996/94‑1 (MQ=60)
                                                       TGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTT                                       >  SRR4302537.1937257/1‑94 (MQ=60)
                                                       TGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTT                                       <  SRR4302537.937251/94‑1 (MQ=60)
                                                        GCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTT                                      <  SRR4302537.3252110/94‑1 (MQ=60)
                                                        GCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTT                                      <  SRR4302537.4127811/94‑1 (MQ=60)
                                                         CGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTG                                     <  SRR4302537.4203126/94‑1 (MQ=60)
                                                         CGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTG                                     >  SRR4302537.2392807/1‑94 (MQ=60)
                                                         CGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTG                                     <  SRR4302537.1943970/94‑1 (MQ=60)
                                                          GTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGG                                    <  SRR4302537.5088860/94‑1 (MQ=60)
                                                          GTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGG                                    >  SRR4302537.5820698/1‑94 (MQ=60)
                                                          GTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGG                                    <  SRR4302537.1106572/94‑1 (MQ=60)
                                                            TTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTG                                  <  SRR4302537.5169141/94‑1 (MQ=60)
                                                            TTTCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTG                                  <  SRR4302537.4399297/94‑1 (MQ=60)
                                                              TCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGT                                >  SRR4302537.2209691/1‑94 (MQ=60)
                                                              TCCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGT                                <  SRR4302537.230936/94‑1 (MQ=60)
                                                               CCAGTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTA                               >  SRR4302537.709102/1‑94 (MQ=60)
                                                                  GTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACG                            <  SRR4302537.1231000/94‑1 (MQ=60)
                                                                  GTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACG                            <  SRR4302537.1669645/94‑1 (MQ=60)
                                                                  GTCAAGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACG                            >  SRR4302537.3194180/1‑94 (MQ=60)
                                                                   TCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGT                           <  SRR4302537.1884812/94‑1 (MQ=60)
                                                                      AGCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACA                        <  SRR4302537.586969/94‑1 (MQ=60)
                                                                       GCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACAT                       <  SRR4302537.3803826/94‑1 (MQ=60)
                                                                       GCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACAT                       <  SRR4302537.2621297/94‑1 (MQ=60)
                                                                       GCATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACAT                       <  SRR4302537.811098/94‑1 (MQ=60)
                                                                        CATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATA                      >  SRR4302537.4527208/1‑94 (MQ=60)
                                                                         ATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATAT                     >  SRR4302537.4912739/1‑94 (MQ=60)
                                                                         ATCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATAT                     >  SRR4302537.2164601/1‑94 (MQ=60)
                                                                          TCTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATC                    <  SRR4302537.70439/94‑1 (MQ=60)
                                                                           CTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCG                   >  SRR4302537.2623931/1‑94 (MQ=60)
                                                                           CTGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCG                   >  SRR4302537.4358399/1‑94 (MQ=60)
                                                                            TGGATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGG                  >  SRR4302537.2873748/1‑94 (MQ=60)
                                                                               ATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGA               >  SRR4302537.6428810/1‑94 (MQ=60)
                                                                               ATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGA               >  SRR4302537.5819148/1‑94 (MQ=60)
                                                                               ATTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGA               <  SRR4302537.1091551/94‑1 (MQ=60)
                                                                                TTTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAA              <  SRR4302537.5319557/94‑1 (MQ=60)
                                                                                 TTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAAC             <  SRR4302537.2831498/94‑1 (MQ=60)
                                                                                 TTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAAC             >  SRR4302537.5393683/1‑94 (MQ=60)
                                                                                 TTCAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAAC             >  SRR4302537.2529798/1‑94 (MQ=60)
                                                                                   CAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTG           >  SRR4302537.2022118/1‑94 (MQ=60)
                                                                                   CAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTG           <  SRR4302537.3009696/94‑1 (MQ=60)
                                                                                   CAATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCCGAGAACTG           >  SRR4302537.2022272/1‑94 (MQ=60)
                                                                                    AATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGC          >  SRR4302537.894440/1‑94 (MQ=60)
                                                                                    AATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGC          >  SRR4302537.2546240/1‑94 (MQ=60)
                                                                                     ATTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCA         >  SRR4302537.5492208/1‑94 (MQ=60)
                                                                                      TTTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCAT        >  SRR4302537.1771373/1‑94 (MQ=60)
                                                                                       TTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATA       >  SRR4302537.5057740/1‑94 (MQ=60)
                                                                                       TTTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATA       <  SRR4302537.409978/94‑1 (MQ=60)
                                                                                        TTCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATAT      <  SRR4302537.5093677/94‑1 (MQ=60)
                                                                                         TCGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATA     <  SRR4302537.5745726/94‑1 (MQ=60)
                                                                                          CGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAG    <  SRR4302537.770808/94‑1 (MQ=60)
                                                                                          CGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAG    >  SRR4302537.5502477/1‑94 (MQ=60)
                                                                                          CGACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAG    <  SRR4302537.489674/94‑1 (MQ=60)
                                                                                           GACGCACCGTGTTTACCCACCCCGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGT   >  SRR4302537.2680603/1‑94 (MQ=60)
                                                                                           GACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGT   <  SRR4302537.2503187/94‑1 (MQ=60)
                                                                                           GACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGT   <  SRR4302537.1777453/94‑1 (MQ=60)
                                                                                           GACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGT   >  SRR4302537.5208120/1‑94 (MQ=60)
                                                                                            ACGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTT  <  SRR4302537.982127/94‑1 (MQ=60)
                                                                                            |                                                                                             
ACCCGGACCGACTTTCCAGTTTTCCAGACCTACACCCGCTGCGGCATCAGTACGCTGCGTTTTCCAGTCAAGCATCTGGATTTCAATTTTCGGCGCACCGTGTTTACCCACCCAGAAATCAGCCTCTGGCGCAAAGGGCAGGAAACCTTTGGTGGTAACGTACATATCGGAGAACTGCATATAGTT  >  NC_000913/3899051‑3899236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑