Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,930,568 +G intergenic (+66/‑373) kup → / → rbsD low affinity potassium transport system/RbsD

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,930,5671.G100.0% 799.8 / NA 231intergenic (+65/‑374)kup/rbsDlow affinity potassium transport system/RbsD
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (107/124);  total (107/124)

Only 100 of 260 total aligned reads displayed.

GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTAT  >  NC_000913/3930475‑3930654
                                                                                             |                                                                                       
 cACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑g                                                                                        <  2:2514411/93‑1 (MQ=255)
  aCCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑gg                                                                                       >  2:283469/1‑92 (MQ=255)
   ccGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑ggc                                                                                      <  1:80852/93‑3 (MQ=255)
    cGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTTAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑ggcg                                                                                     >  2:2312618/1‑90 (MQ=255)
    cGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑ggcg                                                                                     >  1:5713134/1‑90 (MQ=255)
     gAAGACGCTCCATATGTCCATGACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑ggcga                                                                                    >  1:6420196/1‑89 (MQ=255)
       aGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCgaga                                                                                   >  2:5923396/1‑93 (MQ=255)
       aGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCgaga                                                                                   >  1:740855/1‑93 (MQ=255)
           gCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACg                                                                               <  2:4851837/93‑1 (MQ=255)
             tCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGcc                                                                             <  1:5754468/93‑1 (MQ=255)
             tCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGcc                                                                             <  1:3157194/93‑1 (MQ=255)
             tCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGcc                                                                             <  1:231009/93‑1 (MQ=255)
             tCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGcc                                                                             <  1:6357154/93‑1 (MQ=255)
              ccATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCa                                                                            >  1:2816416/1‑93 (MQ=255)
              ccATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCa                                                                            <  2:1932595/93‑1 (MQ=255)
                atatGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCaaa                                                                          <  1:4060440/93‑1 (MQ=255)
                 tatGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAc                                                                         >  2:3333329/1‑93 (MQ=255)
                 tatGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGACGCGTGGTGGCAAGTTCTTGTTGGCGGGAAAGGCCacaa                                                                         >  2:4885973/1‑90 (MQ=255)
                   tGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGGAACGCCAAACgg                                                                       >  2:1828978/1‑92 (MQ=255)
                    gTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTa                                                                      <  1:4732286/93‑1 (MQ=255)
                    gTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTa                                                                      >  1:3433364/1‑93 (MQ=255)
                     tCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTag                                                                     >  1:354358/1‑93 (MQ=255)
                      ccATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTaga                                                                    <  2:925825/93‑1 (MQ=255)
                       cATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaa                                                                   >  2:6058131/1‑93 (MQ=255)
                       cATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaa                                                                   <  1:4941084/93‑1 (MQ=255)
                        aTAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaag                                                                  <  1:2963031/93‑1 (MQ=255)
                        aTAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaag                                                                  <  2:2932816/93‑1 (MQ=255)
                         tAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTagaaga                                                                 <  2:1471616/93‑1 (MQ=255)
                           aCGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGaagaag                                                               <  2:1714153/93‑1 (MQ=255)
                           aCGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGaagaag                                                               <  2:521402/93‑1 (MQ=255)
                           aCGTCCGTCGGGTACAGATAGAACAACTGTCGCCGACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGaagaag                                                               <  2:4755710/93‑1 (MQ=255)
                             gtccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGtt                                                             <  2:272930/93‑1 (MQ=255)
                              tccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGttt                                                            >  1:4229374/1‑93 (MQ=255)
                              tccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGttt                                                            >  2:722369/1‑93 (MQ=255)
                              tccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGttt                                                            >  2:3246661/1‑93 (MQ=255)
                               ccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGtttt                                                           <  1:4888088/93‑1 (MQ=255)
                               ccgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGtttt                                                           >  2:1653186/1‑93 (MQ=255)
                               ccgtcGGGTACAGATTGAACAACTGTCGCCCACATTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGtttt                                                           >  1:6308713/1‑93 (MQ=255)
                                cgtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAGCGTAGAAGAAGTTTTc                                                          <  2:313159/93‑1 (MQ=255)
                                 gtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTcc                                                         >  2:4239880/1‑93 (MQ=255)
                                 gtcGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTcc                                                         >  1:5033847/1‑93 (MQ=255)
                                   cGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCAc                                                       <  1:613959/93‑1 (MQ=255)
                                   cGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCAc                                                       <  1:1864745/93‑1 (MQ=255)
                                    gggTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGGGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCAcc                                                      >  1:4558112/1‑93 (MQ=255)
                                      gTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGc                                                    <  2:5641149/93‑1 (MQ=255)
                                      gTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGc                                                    <  1:4413127/93‑1 (MQ=255)
                                       tACAGATTGAACAACTGTCGCGCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCTAGAAACGCCAGACGTAGACGAAGTTTTCCACCGCt                                                   <  1:5958957/93‑1 (MQ=255)
                                       tACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCt                                                   >  1:3981352/1‑93 (MQ=255)
                                       tACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCt                                                   <  2:3736708/93‑1 (MQ=255)
                                       tACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCt                                                   <  1:4583154/93‑1 (MQ=255)
                                        aCAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTg                                                  <  2:1241342/93‑1 (MQ=255)
                                         cAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGc                                                 <  2:2236529/93‑1 (MQ=255)
                                          aGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCg                                                <  2:4297990/93‑1 (MQ=255)
                                          aGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCg                                                >  2:2626392/1‑93 (MQ=255)
                                           gATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCgg                                               <  1:1303178/93‑1 (MQ=255)
                                            aTTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGt                                              >  1:2638394/1‑93 (MQ=255)
                                             ttGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTc                                             >  1:1470946/1‑93 (MQ=255)
                                              tGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCt                                            >  1:3610036/1‑93 (MQ=255)
                                                aacaacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTgg                                          >  1:712009/1‑93 (MQ=255)
                                                 acaacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGa                                         <  1:4997556/93‑1 (MQ=255)
                                                 acaacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGa                                         <  1:5616939/93‑1 (MQ=255)
                                                 acaacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGa                                         >  2:2166977/1‑93 (MQ=255)
                                                  caacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGaa                                        >  2:4457964/1‑93 (MQ=255)
                                                  caacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGaa                                        <  1:5905337/93‑1 (MQ=255)
                                                   aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAg                                       <  2:1673752/93‑1 (MQ=255)
                                                   aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAg                                       <  1:1704202/93‑1 (MQ=255)
                                                   aacTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGCAAg                                       >  2:3701807/1‑93 (MQ=255)
                                                     cTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGa                                     <  2:6407094/93‑1 (MQ=255)
                                                          gCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAg                                >  2:2390064/1‑93 (MQ=255)
                                                            ccACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGtt                              <  1:4300489/93‑1 (MQ=255)
                                                            ccACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGtt                              >  2:4953269/1‑93 (MQ=255)
                                                            caaCTTTCTGGCGCGTGGTGACAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGtt                              <  1:1997334/91‑1 (MQ=255)
                                                             cACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTg                             >  1:5487426/1‑93 (MQ=255)
                                                             cACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTg                             >  2:1223068/1‑93 (MQ=255)
                                                             cACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTg                             >  2:5837698/1‑93 (MQ=255)
                                                                tttCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCg                          <  1:2551385/93‑1 (MQ=255)
                                                                tttCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCg                          >  2:1157248/1‑93 (MQ=255)
                                                                   cTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgat                       <  1:1923033/93‑1 (MQ=255)
                                                                    tGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatg                      <  1:3724113/93‑1 (MQ=255)
                                                                     ggCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatga                     <  2:704033/93‑1 (MQ=255)
                                                                     ggCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatga                     <  2:1021099/93‑1 (MQ=255)
                                                                     ggCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatga                     >  1:239587/1‑93 (MQ=255)
                                                                      gcgcGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGggtgat                    >  2:6255397/1‑93 (MQ=255)
                                                                      gcgcGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatgat                    <  2:4527402/93‑1 (MQ=255)
                                                                      gcgcGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGgatgat                    <  2:6006771/93‑1 (MQ=255)
                                                                        gcgTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATgg                  <  2:2042130/93‑1 (MQ=255)
                                                                         cgTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGa                 <  1:2133341/93‑1 (MQ=255)
                                                                         cgTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGa                 <  1:5846672/93‑1 (MQ=255)
                                                                             gtggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcc             <  1:1219478/93‑1 (MQ=255)
                                                                             gtggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcc             >  2:5749267/1‑93 (MQ=255)
                                                                              tggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcct            <  1:6200774/93‑1 (MQ=255)
                                                                               ggCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAAcctc           <  2:2105043/93‑1 (MQ=255)
                                                                                   aGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCcttc       <  1:2492361/93‑1 (MQ=255)
                                                                                    gTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCcttct      >  2:3611958/1‑93 (MQ=255)
                                                                                     ttATGGTTGGCGAGAAACGCCAAACTTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGACGATGGAAACCTCcttctt     <  2:3848282/93‑1 (MQ=255)
                                                                                     ttATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCcttctt     >  1:1122102/1‑93 (MQ=255)
                                                                                      tATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCttt    <  2:402138/93‑1 (MQ=255)
                                                                                       aTGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTa   <  2:1696470/93‑1 (MQ=255)
                                                                                        tGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTAt  >  1:5096902/1‑93 (MQ=255)
                                                                                        tGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTAt  <  1:1081416/93‑1 (MQ=255)
                                                                                             |                                                                                       
GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTAT  >  NC_000913/3930475‑3930654

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  >  NC_000913/3930475‑3930661
                                                                                              |                                                                                             
GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑G                                                                                               <  SRR4302537.2463491/94‑1 (MQ=60)
GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑G                                                                                               >  SRR4302537.3467641/1‑94 (MQ=60)
 CACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GG                                                                                              >  SRR4302537.2792480/1‑94 (MQ=60)
  ACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑Ggc                                                                                             <  SRR4302537.5162104/94‑3 (MQ=60)
  ACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GGG                                                                                             >  SRR4302537.4605703/1‑94 (MQ=60)
   CCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑Ggcg                                                                                            <  SRR4302537.81446/94‑4 (MQ=60)
   CCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑Ggcg                                                                                            >  SRR4302537.5752234/1‑91 (MQ=60)
    CGAAGACGCTCCATATGTCCATGACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGA                                                                                            >  SRR4302537.6463841/1‑94 (MQ=60)
     GAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAG                                                                                           <  SRR4302537.6195429/94‑1 (MQ=60)
      AAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGA                                                                                          <  SRR4302537.4051028/94‑1 (MQ=60)
      AAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGA                                                                                          >  SRR4302537.745915/1‑94 (MQ=60)
      AAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGA                                                                                          >  SRR4302537.1889304/1‑94 (MQ=60)
       AGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGGGGCAAGTTATGGTTGGCGAGAA                                                                                         >  SRR4302537.5042465/1‑94 (MQ=60)
         ACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAAC                                                                                       <  SRR4302537.429477/94‑1 (MQ=60)
          CGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACG                                                                                      <  SRR4302537.4478540/94‑1 (MQ=60)
          CGCTCCATATGTCCATAACGTCCGTCGGGTACAGATGGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACG                                                                                      <  SRR4302537.3145912/94‑1 (MQ=60)
            CTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCC                                                                                    >  SRR4302537.6095865/1‑94 (MQ=60)
            CTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCC                                                                                    >  SRR4302537.222042/1‑94 (MQ=60)
             TCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCA                                                                                   <  SRR4302537.3179356/94‑1 (MQ=60)
             TCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCA                                                                                   <  SRR4302537.6400542/94‑1 (MQ=60)
             TCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCA                                                                                   <  SRR4302537.5793883/94‑1 (MQ=60)
             TCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCA                                                                                   >  SRR4302537.2836108/1‑94 (MQ=60)
             TCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCA                                                                                   <  SRR4302537.232638/94‑1 (MQ=60)
             TCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCA                                                                                   >  SRR4302537.894772/1‑94 (MQ=60)
              CCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAA                                                                                  <  SRR4302537.1542852/94‑1 (MQ=60)
               CATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAA                                                                                 <  SRR4302537.2145585/94‑1 (MQ=60)
               CATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAA                                                                                 >  SRR4302537.5789332/1‑94 (MQ=60)
                ATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAC                                                                                <  SRR4302537.4088816/94‑1 (MQ=60)
                ATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAC                                                                                <  SRR4302537.1689088/94‑1 (MQ=60)
                ATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAC                                                                                <  SRR4302537.2623343/94‑1 (MQ=60)
                ATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAC                                                                                <  SRR4302537.4352207/94‑1 (MQ=60)
                ATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAAC                                                                                <  SRR4302537.729245/94‑1 (MQ=60)
                   TGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTA                                                                             >  SRR4302537.3457666/1‑94 (MQ=60)
                    GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAG                                                                            >  SRR4302537.356825/1‑94 (MQ=60)
                    GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAG                                                                            <  SRR4302537.2849254/94‑1 (MQ=60)
                    GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAG                                                                            <  SRR4302537.112865/94‑1 (MQ=60)
                    GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAG                                                                            <  SRR4302537.4764983/94‑1 (MQ=60)
                    GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAG                                                                            <  SRR4302537.1860974/94‑1 (MQ=60)
                    GTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAG                                                                            <  SRR4302537.5914665/94‑1 (MQ=60)
                     TCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGA                                                                           >  SRR4302537.3624316/1‑94 (MQ=60)
                       CATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAG                                                                         <  SRR4302537.4975157/94‑1 (MQ=60)
                        ATAACGTCCGTCGGGTACAGATTGAATAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGA                                                                        <  SRR4302537.774434/94‑1 (MQ=60)
                        ATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGA                                                                        <  SRR4302537.2983830/94‑1 (MQ=60)
                         TAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAA                                                                       <  SRR4302537.5700205/94‑1 (MQ=60)
                          AACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAG                                                                      >  SRR4302537.3545396/1‑94 (MQ=60)
                          AACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTAGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAG                                                                      >  SRR4302537.4581280/1‑94 (MQ=60)
                             GTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTT                                                                   >  SRR4302537.4258821/1‑94 (MQ=60)
                              TCCGTCGGGTACAGATTGAACAACTGTCGCCCACATTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTT                                                                  >  SRR4302537.6351909/1‑94 (MQ=60)
                               CCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTC                                                                 <  SRR4302537.4921841/94‑1 (MQ=60)
                                CGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCC                                                                >  SRR4302537.5068501/1‑94 (MQ=60)
                                   CGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACC                                                             <  SRR4302537.1877732/94‑1 (MQ=60)
                                   CGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACC                                                             <  SRR4302537.2693095/94‑1 (MQ=60)
                                   CGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACC                                                             <  SRR4302537.618147/94‑1 (MQ=60)
                                   CGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGGGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACC                                                             >  SRR4302537.4589666/1‑94 (MQ=60)
                                      GTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCT                                                          <  SRR4302537.4443584/94‑1 (MQ=60)
                                      GTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCT                                                          >  SRR4302537.4009224/1‑94 (MQ=60)
                                       TACAGATTGAACAACTGTCGCGCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCTAGAAACGCCAGACGTAGACGAAGTTTTCCACCGCTG                                                         <  SRR4302537.5999859/94‑1 (MQ=60)
                                       TACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTG                                                         <  SRR4302537.4614854/94‑1 (MQ=60)
                                        ACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGC                                                        <  SRR4302537.5376147/94‑1 (MQ=60)
                                        ACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGC                                                        <  SRR4302537.4776075/94‑1 (MQ=60)
                                         CAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCG                                                       >  SRR4302537.2079071/1‑94 (MQ=60)
                                          AGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGG                                                      >  SRR4302537.1320507/1‑94 (MQ=60)
                                           GATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGT                                                     <  SRR4302537.1312239/94‑1 (MQ=60)
                                           GATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGT                                                     >  SRR4302537.4804096/1‑94 (MQ=60)
                                           GATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGT                                                     >  SRR4302537.2656852/1‑94 (MQ=60)
                                            ATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTC                                                    >  SRR4302537.1481171/1‑94 (MQ=60)
                                             TTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCT                                                   >  SRR4302537.3635484/1‑94 (MQ=60)
                                             TTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCT                                                   <  SRR4302537.4979969/94‑1 (MQ=60)
                                              TGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTG                                                  >  SRR4302537.3858210/1‑94 (MQ=60)
                                               GAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGG                                                 >  SRR4302537.898707/1‑94 (MQ=60)
                                               GAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGG                                                 >  SRR4302537.716883/1‑94 (MQ=60)
                                               GAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGG                                                 <  SRR4302537.5959280/94‑1 (MQ=60)
                                                AACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGA                                                <  SRR4302537.6395231/94‑1 (MQ=60)
                                                AACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGA                                                >  SRR4302537.4937163/1‑94 (MQ=60)
                                                 ACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAA                                               <  SRR4302537.5655346/94‑1 (MQ=60)
                                                 ACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAA                                               <  SRR4302537.5031993/94‑1 (MQ=60)
                                                  CAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAG                                              <  SRR4302537.5945859/94‑1 (MQ=60)
                                                   AACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGG                                             >  SRR4302537.3381151/1‑94 (MQ=60)
                                                   AACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGG                                             <  SRR4302537.4654110/94‑1 (MQ=60)
                                                   AACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGG                                             <  SRR4302537.1716116/94‑1 (MQ=60)
                                                    ACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGA                                            <  SRR4302537.4018278/94‑1 (MQ=60)
                                                      TGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATT                                          <  SRR4302537.6063787/94‑1 (MQ=60)
                                                      TGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATT                                          >  SRR4302537.6272017/1‑94 (MQ=60)
                                                           CCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTT                                     >  SRR4302537.1925156/1‑94 (MQ=60)
                                                           CCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTT                                     >  SRR4302537.5697735/1‑94 (MQ=60)
                                                           CCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTT                                     <  SRR4302537.3670819/94‑1 (MQ=60)
                                                            CCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTG                                    <  SRR4302537.4330377/94‑1 (MQ=60)
                                                            CCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTG                                    >  SRR4302537.5524883/1‑94 (MQ=60)
                                                            CCACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTG                                    <  SRR4302537.4256889/94‑1 (MQ=60)
                                                            CAACTTTCTGGCGCGTGGTGACAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTG                                    <  SRR4302537.2011222/94‑1 (MQ=60)
                                                              ACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCC                                  >  SRR4302537.3626925/1‑94 (MQ=60)
                                                              ACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCC                                  <  SRR4302537.5580851/94‑1 (MQ=60)
                                                              ACTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCC                                  <  SRR4302537.5855984/94‑1 (MQ=60)
                                                               CTTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCG                                 >  SRR4302537.2385802/1‑94 (MQ=60)
                                                                TTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGG                                <  SRR4302537.2569253/94‑1 (MQ=60)
                                                                TTTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGG                                <  SRR4302537.2523032/94‑1 (MQ=60)
                                                                 TTCTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGA                               <  SRR4302537.6424089/94‑1 (MQ=60)
                                                                   CTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATG                             <  SRR4302537.1936387/94‑1 (MQ=60)
                                                                   CTGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATG                             <  SRR4302537.5855079/94‑1 (MQ=60)
                                                                    TGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGA                            <  SRR4302537.3750280/94‑1 (MQ=60)
                                                                    TGGCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGA                            >  SRR4302537.241283/1‑94 (MQ=60)
                                                                      GCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATG                          <  SRR4302537.196035/94‑1 (MQ=60)
                                                                      GCGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATG                          >  SRR4302537.127509/1‑94 (MQ=60)
                                                                       CGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGG                         <  SRR4302537.6475167/94‑1 (MQ=60)
                                                                       CGCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGG                         <  SRR4302537.2428391/94‑1 (MQ=60)
                                                                        GCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGA                        <  SRR4302537.787618/94‑1 (MQ=60)
                                                                        GCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGA                        >  SRR4302537.625568/1‑94 (MQ=60)
                                                                        GCGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGA                        <  SRR4302537.1227775/94‑1 (MQ=60)
                                                                         CGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAA                       >  SRR4302537.2213989/1‑94 (MQ=60)
                                                                         CGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAA                       <  SRR4302537.5886762/94‑1 (MQ=60)
                                                                         CGTGGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAA                       <  SRR4302537.2148355/94‑1 (MQ=60)
                                                                            GGTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACC                    <  SRR4302537.3001139/94‑1 (MQ=60)
                                                                             GTGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCT                   <  SRR4302537.1227961/94‑1 (MQ=60)
                                                                              TGGCAAGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTC                  <  SRR4302537.6243508/94‑1 (MQ=60)
                                                                                   AGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCT             <  SRR4302537.5943738/94‑1 (MQ=60)
                                                                                   AGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCT             <  SRR4302537.2509910/94‑1 (MQ=60)
                                                                                   AGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCT             <  SRR4302537.4718126/94‑1 (MQ=60)
                                                                                   AGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCT             >  SRR4302537.136659/1‑94 (MQ=60)
                                                                                   AGTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCT             >  SRR4302537.12115/1‑94 (MQ=60)
                                                                                    GTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTT            >  SRR4302537.653216/1‑94 (MQ=60)
                                                                                    GTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTT            >  SRR4302537.1129881/1‑94 (MQ=60)
                                                                                    GTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTT            <  SRR4302537.2647391/94‑1 (MQ=60)
                                                                                    GTTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTT            >  SRR4302537.4242061/1‑94 (MQ=60)
                                                                                     TTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTT           >  SRR4302537.3957298/1‑94 (MQ=60)
                                                                                     TTATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTT           >  SRR4302537.6389465/1‑94 (MQ=60)
                                                                                      TATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTA          >  SRR4302537.5015418/1‑94 (MQ=60)
                                                                                       ATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTAT         >  SRR4302537.5132001/1‑94 (MQ=60)
                                                                                       ATGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTAT         >  SRR4302537.5128340/1‑94 (MQ=60)
                                                                                        TGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATG        <  SRR4302537.1088884/94‑1 (MQ=60)
                                                                                        TGGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATG        <  SRR4302537.5112844/94‑1 (MQ=60)
                                                                                         GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGT       >  SRR4302537.5235922/1‑94 (MQ=60)
                                                                                         GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGT       >  SRR4302537.4917139/1‑94 (MQ=60)
                                                                                         GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGT       <  SRR4302537.577862/94‑1 (MQ=60)
                                                                                         GGTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGT       <  SRR4302537.2074991/94‑1 (MQ=60)
                                                                                          GTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTC      <  SRR4302537.6210053/94‑1 (MQ=60)
                                                                                          GTTGGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTC      <  SRR4302537.75288/94‑1 (MQ=60)
                                                                                           ttgGCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCG     <  SRR4302537.6157058/91‑1 (MQ=60)
                                                                                           TG‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGC    <  SRR4302537.1533707/94‑1 (MQ=60)
                                                                                              GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  <  SRR4302537.4032684/94‑1 (MQ=60)
                                                                                              GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  <  SRR4302537.5625396/94‑1 (MQ=60)
                                                                                              |                                                                                             
GCACCGAAGACGCTCCATATGTCCATAACGTCCGTCGGGTACAGATTGAACAACTGTCGCCCACTTTCTGGCGCGTGGTGGCAAGTTATGGTT‑GCGAGAAACGCCAAACGTAGAAGAAGTTTTCCACCGCTGCGGTCTGGAAGGATTAAGTTGCCGGATGATGGAAACCTCCTTCTTTATGTCGCAT  >  NC_000913/3930475‑3930661

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑