Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I2 R1	882	254.4	12957588	98.0%	12698436	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,069	A→G	L43L (TTA→TTG)	rbsD →	RbsD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,069	0	A	G	95.5%	831.7 / 18.7	267	L43L (TTA→TTG)	rbsD	RbsD
Reads supporting (aligned to +/- strand): ref base A (8/4); new base G (136/119); total (144/123)
Fisher's exact test for biased strand distribution p-value = 3.95e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.92e-01

Only 100 of 276 total aligned reads displayed.

GAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGA  >  NC_000913/3930979‑3931158

gAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGcc                                                                                         >  1:681424/1‑93 (MQ=255)

 aaaaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGccc                                                                                        <  2:1824027/93‑1 (MQ=255)

  aaaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCa                                                                                       >  1:1045064/1‑93 (MQ=255)

  aaaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCa                                                                                       <  2:2435939/93‑1 (MQ=255)

  aaaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCa                                                                                       >  2:5306715/1‑93 (MQ=255)

  aaaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCa                                                                                       >  1:2144854/1‑93 (MQ=255)

   aaaaGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCAt                                                                                      <  2:6341101/93‑1 (MQ=255)

       ggCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATcccc                                                                                  >  2:1427026/1‑93 (MQ=255)

       ggCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATcccc                                                                                  >  2:1023488/1‑93 (MQ=255)

        gCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCa                                                                                 >  1:1765522/1‑93 (MQ=255)

         cACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCaa                                                                                >  2:1320598/1‑93 (MQ=255)

         cACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGGGATGCTGGTTTGCCCATCCCCaa                                                                                >  2:578492/1‑93 (MQ=255)

           ccGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCaaaa                                                                              >  2:4087474/1‑93 (MQ=255)

           ccGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCaaaa                                                                              >  2:2882366/1‑93 (MQ=255)

           ccGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCaaaa                                                                              >  1:3367820/1‑93 (MQ=255)

           ccGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCaaaa                                                                              >  1:5613163/1‑93 (MQ=255)

            cGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAg                                                                             >  2:2850407/1‑93 (MQ=255)

              ttCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTa                                                                           <  1:6259781/93‑1 (MQ=255)

               tCTTAATTTTGATATTTCACCGGTGATCTCCCGTCTGGGACGTACCGATACGCTGGTGGTGTGTGATGCTGGTTTGTCCATCCCCCAAAGTAc                                                                          >  2:6275533/1‑93 (MQ=255)

               tCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTAc                                                                          <  1:1956027/93‑1 (MQ=255)

               tCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTAc                                                                          >  1:5746518/1‑93 (MQ=255)

               tCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTAc                                                                          >  2:6422929/1‑93 (MQ=255)

               tCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTAc                                                                          >  2:2634531/1‑93 (MQ=255)

                cTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACa                                                                         >  1:1110686/1‑93 (MQ=255)

                  tAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAAc                                                                       >  2:5677453/1‑93 (MQ=255)

                    aTTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAAcgc                                                                     <  2:3265696/93‑1 (MQ=255)

                    aTTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAAcgc                                                                     >  1:5394314/1‑93 (MQ=255)

                     ttCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAAcgcg                                                                    >  1:4947527/1‑93 (MQ=255)

                      tCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGt                                                                   >  2:5886218/1‑93 (MQ=255)

                       cTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTa                                                                  <  1:4521973/93‑1 (MQ=255)

                         gATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTAtg                                                                >  2:1926330/1‑92 (MQ=255)

                         gATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATc                                                                <  2:5046336/93‑1 (MQ=255)

                         gATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGAGGTGTGTGATGCTGGTTTGCCCATCACCAAAAGTACAACGCGTATc                                                                >  2:2206885/1‑93 (MQ=255)

                           tatTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGa                                                              <  1:883588/93‑1 (MQ=255)

                           tatTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGa                                                              >  2:1140513/1‑93 (MQ=255)

                            atTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGat                                                             <  2:4942700/93‑1 (MQ=255)

                             tttCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGata                                                            >  2:1989939/1‑93 (MQ=255)

                             tttCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGata                                                            >  1:4786930/1‑93 (MQ=255)

                             tttCATCGGGGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGatt                                                            >  2:4814604/1‑92 (MQ=255)

                              ttCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGatat                                                           <  1:4419204/93‑1 (MQ=255)

                                cATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATgg                                                         <  2:1775534/93‑1 (MQ=255)

                                 aTCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGc                                                        <  2:2992284/93‑1 (MQ=255)

                                 aTCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGc                                                        >  1:2350457/1‑93 (MQ=255)

                                  tCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCa                                                       >  1:2890425/1‑93 (MQ=255)

                                   cGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCAt                                                      <  1:238028/93‑1 (MQ=255)

                                    ggTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCAtt                                                     >  1:3231444/1‑93 (MQ=255)

                                     gTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTa                                                    >  1:2461670/1‑93 (MQ=255)

                                     gTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTAATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTa                                                    >  1:3471902/1‑93 (MQ=255)

                                      tGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTaa                                                   >  2:2681003/1‑93 (MQ=255)

                                      tGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTaa                                                   <  2:5518587/93‑1 (MQ=255)

                                       gacctcCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAAc                                                  <  1:6148076/90‑1 (MQ=255)

                                       gATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAAc                                                  <  1:307200/93‑1 (MQ=255)

                                       gATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAAc                                                  <  1:586156/93‑1 (MQ=255)

                                       gATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAAc                                                  >  2:4039445/1‑93 (MQ=255)

                                         tctcCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAAccc                                                <  2:2416619/93‑1 (MQ=255)

                                         tctcCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAAccc                                                <  2:4005311/93‑1 (MQ=255)

                                           tcCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAg                                              <  1:4841875/93‑1 (MQ=255)

                                           tcCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAg                                              >  2:200993/1‑93 (MQ=255)

                                            cccGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAgg                                             >  1:1427728/1‑93 (MQ=255)

                                             ccGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAggg                                            >  2:84286/1‑93 (MQ=255)

                                             ccGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAggg                                            >  1:2846902/1‑93 (MQ=255)

                                             ccGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAggg                                            >  2:119053/1‑93 (MQ=255)

                                              cGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGgt                                           >  1:3088318/1‑93 (MQ=255)

                                              cGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGgt                                           <  1:698676/93‑1 (MQ=255)

                                              cGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGgt                                           >  1:2569125/1‑93 (MQ=255)

                                              cGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGgt                                           <  1:944868/93‑1 (MQ=255)

                                                tCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGgtgt                                         >  1:4675737/1‑93 (MQ=255)

                                                  tGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTAc                                       >  1:1950861/1‑93 (MQ=255)

                                                  tGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTAc                                       <  2:4668889/93‑1 (MQ=255)

                                                  tGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTAc                                       <  2:3843178/93‑1 (MQ=255)

                                                       cATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACcttct                                  >  2:4617646/1‑93 (MQ=255)

                                                        aTACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACcttctt                                 >  1:1242016/1‑93 (MQ=255)

                                                         tACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCttt                                >  1:1623849/1‑93 (MQ=255)

                                                          aCCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCtttt                               <  1:3827942/93‑1 (MQ=255)

                                                           ccGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTa                              >  2:5993901/1‑93 (MQ=255)

                                                           ccGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTa                              <  1:5020919/93‑1 (MQ=255)

                                                            cGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTAt                             <  1:3013568/93‑1 (MQ=255)

                                                            cGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTAt                             <  2:3671701/93‑1 (MQ=255)

                                                             gATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATg                            <  1:821929/93‑1 (MQ=255)

                                                             gATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATg                            <  2:5955995/93‑1 (MQ=255)

                                                             gATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATg                            <  1:2220559/93‑1 (MQ=255)

                                                              aTACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATga                           >  1:5250008/1‑92 (MQ=255)

                                                              aTACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGc                           >  1:4066488/1‑93 (MQ=255)

                                                               tACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCa                          >  1:5708446/1‑93 (MQ=255)

                                                                aCGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAg                         <  2:3336595/93‑1 (MQ=255)

                                                                    tggtggTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGc                     <  1:6065302/93‑1 (MQ=255)

                                                                     ggtggtGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCt                    <  2:436149/93‑1 (MQ=255)

                                                                      gtggtgTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTg                   <  1:6336645/93‑1 (MQ=255)

                                                                       tggtgTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTgg                  <  1:4641899/93‑1 (MQ=255)

                                                                         gtgtgtGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGc                <  2:5148568/93‑1 (MQ=255)

                                                                           gtgtgATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGcgt              >  2:3117760/1‑93 (MQ=255)

                                                                            tgtgATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGcgtc             <  1:4360890/93‑1 (MQ=255)

                                                                            tgtgATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGcgtc             <  2:6173407/93‑1 (MQ=255)

                                                                                aTGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTca         <  2:209635/93‑1 (MQ=255)

                                                                                aTGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTca         <  2:3515903/93‑1 (MQ=255)

                                                                                    tGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaa     <  2:611964/93‑1 (MQ=255)

                                                                                    tGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaa     >  1:5888963/1‑93 (MQ=255)

                                                                                     ggTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACcaat    >  2:2732640/1‑93 (MQ=255)

                                                                                       tttGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaatga  <  2:1631799/93‑1 (MQ=255)

                                                                                       tttGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACaaatga  <  2:4410260/93‑1 (MQ=255)

GAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGA  >  NC_000913/3930979‑3931158

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 29 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATG  >  NC_000913/3930976‑3931162

AATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTG                                                                                               >  SRR4302537.2115495/1‑94 (MQ=60)

 ATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGC                                                                                              <  SRR4302537.5408061/94‑1 (MQ=60)

  TGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCC                                                                                             >  SRR4302537.686091/1‑94 (MQ=60)

    AAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCA                                                                                           >  SRR4302537.1052276/1‑94 (MQ=60)

    AAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCA                                                                                           >  SRR4302537.2159943/1‑94 (MQ=60)

      AAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATC                                                                                         >  SRR4302537.6046273/1‑94 (MQ=60)

        AAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCC                                                                                       >  SRR4302537.2617328/1‑94 (MQ=60)

        AAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCC                                                                                       >  SRR4302537.2445372/1‑94 (MQ=60)

         AGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCC                                                                                      >  SRR4302537.2452621/1‑94 (MQ=60)

         AGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCC                                                                                      >  SRR4302537.1110540/1‑94 (MQ=60)

         AGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGCACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCC                                                                                      >  SRR4302537.3251756/1‑94 (MQ=60)

          GGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCA                                                                                     >  SRR4302537.1777816/1‑94 (MQ=60)

           GCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAA                                                                                    >  SRR4302537.4915488/1‑94 (MQ=60)

             CCCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAA                                                                                  <  SRR4302537.4842324/94‑1 (MQ=60)

             ACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAA                                                                                  >  SRR4302537.5651541/1‑94 (MQ=60)

             ACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAA                                                                                  >  SRR4302537.3391677/1‑94 (MQ=60)

             ACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAA                                                                                  >  SRR4302537.616143/1‑94 (MQ=60)

              CCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAG                                                                                 >  SRR4302537.2388546/1‑94 (MQ=60)

              CCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAG                                                                                 <  SRR4302537.695539/94‑1 (MQ=60)

               CGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAACAGT                                                                                >  SRR4302537.6125879/1‑94 (MQ=60)

               CGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGT                                                                                <  SRR4302537.1710544/94‑1 (MQ=60)

               CGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGA                                                                                >  SRR4302537.634796/1‑94 (MQ=60)

                GTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTA                                                                               >  SRR4302537.937752/1‑94 (MQ=60)

                GTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTA                                                                               <  SRR4302537.5779378/94‑1 (MQ=60)

                 TTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTAC                                                                              <  SRR4302537.6302763/94‑1 (MQ=60)

                 TTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTAC                                                                              >  SRR4302537.5785871/1‑94 (MQ=60)

                  TCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACA                                                                             >  SRR4302537.1118358/1‑94 (MQ=60)

                  TCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACA                                                                             <  SRR4302537.1969634/94‑1 (MQ=60)

                  TCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACA                                                                             >  SRR4302537.1965424/1‑94 (MQ=60)

                    TTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAAC                                                                           >  SRR4302537.1250634/1‑94 (MQ=60)

                    TTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGGGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAAC                                                                           >  SRR4302537.2283674/1‑94 (MQ=60)

                     TAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACG                                                                          <  SRR4302537.4408168/94‑1 (MQ=60)

                      AATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGC                                                                         >  SRR4302537.3837734/1‑94 (MQ=60)

                      AATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGC                                                                         >  SRR4302537.6414730/1‑94 (MQ=60)

                      AATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGC                                                                         >  SRR4302537.112119/1‑94 (MQ=60)

                      AATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGC                                                                         >  SRR4302537.5431152/1‑94 (MQ=60)

                      AATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGC                                                                         >  SRR4302537.5531057/1‑94 (MQ=60)

                      AATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTAGTTTGCCCATCCCCAACAGTACAACGC                                                                         >  SRR4302537.5131141/1‑94 (MQ=60)

                       ATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCG                                                                        >  SRR4302537.4981636/1‑94 (MQ=60)

                          CTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTAT                                                                     >  SRR4302537.3234331/1‑94 (MQ=60)

                          CTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTAT                                                                     <  SRR4302537.4986494/94‑1 (MQ=60)

                          CTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTAT                                                                     >  SRR4302537.5046620/1‑94 (MQ=60)

                          CTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTAT                                                                     >  SRR4302537.694321/1‑94 (MQ=60)

                          CTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTAT                                                                     <  SRR4302537.6359572/94‑1 (MQ=60)

                          CTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTAT                                                                     <  SRR4302537.4553256/94‑1 (MQ=60)

                           TGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATC                                                                    >  SRR4302537.994388/1‑94 (MQ=60)

                             ATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGA                                                                  >  SRR4302537.3770605/1‑94 (MQ=60)

                              TATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGAT                                                                 <  SRR4302537.3886501/94‑1 (MQ=60)

                              TATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGAT                                                                 <  SRR4302537.889668/94‑1 (MQ=60)

                               ATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATA                                                                >  SRR4302537.4818711/1‑94 (MQ=60)

                               ATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATA                                                                >  SRR4302537.4819967/1‑94 (MQ=60)

                               ATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGCGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATA                                                                >  SRR4302537.866168/1‑94 (MQ=60)

                                TTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATAT                                                               <  SRR4302537.4679604/94‑1 (MQ=60)

                                 TTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATG                                                              >  SRR4302537.818267/1‑94 (MQ=60)

                                 TTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATG                                                              <  SRR4302537.4449714/94‑1 (MQ=60)

                                  TCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGG                                                             <  SRR4302537.5743203/94‑1 (MQ=60)

                                   CATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGC                                                            >  SRR4302537.2367006/1‑94 (MQ=60)

                                    ATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCA                                                           >  SRR4302537.2910714/1‑94 (MQ=60)

                                     TCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCAATATGGCAT                                                          <  SRR4302537.3964685/94‑1 (MQ=60)

                                     TCGGTGATCTCCCGGCTGGGACATACCGATACGCTGGTGGTGTGTGATTCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCAT                                                          <  SRR4302537.420256/94‑1 (MQ=60)

                                      CGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATT                                                         <  SRR4302537.239710/94‑1 (MQ=60)

                                      CGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATT                                                         <  SRR4302537.4971343/94‑1 (MQ=60)

                                      CGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATT                                                         >  SRR4302537.5193998/1‑94 (MQ=60)

                                      CGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATT                                                         >  SRR4302537.3254216/1‑94 (MQ=60)

                                      CGGTGATCGCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATT                                                         <  SRR4302537.1559060/94‑1 (MQ=60)

                                       GGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTA                                                        >  SRR4302537.4439222/1‑94 (MQ=60)

                                       GGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTA                                                        <  SRR4302537.2285759/94‑1 (MQ=60)

                                       GGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTA                                                        >  SRR4302537.2478983/1‑94 (MQ=60)

                                       GGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTAATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTA                                                        >  SRR4302537.3496470/1‑94 (MQ=60)

                                        GTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAA                                                       <  SRR4302537.3386226/94‑1 (MQ=60)

                                        GTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAA                                                       >  SRR4302537.4474643/1‑94 (MQ=60)

                                         TGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGGTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAAC                                                      >  SRR4302537.5901759/1‑94 (MQ=60)

                                          GATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACC                                                     <  SRR4302537.590158/94‑1 (MQ=60)

                                          GATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACC                                                     <  SRR4302537.4184647/94‑1 (MQ=60)

                                          GATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACC                                                     >  SRR4302537.1965752/1‑94 (MQ=60)

                                          GATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACC                                                     <  SRR4302537.5475906/94‑1 (MQ=60)

                                          GATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACC                                                     <  SRR4302537.309330/94‑1 (MQ=60)

                                          GACCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACC                                                     <  SRR4302537.6190363/94‑1 (MQ=60)

                                           ATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCC                                                    >  SRR4302537.4686501/1‑94 (MQ=60)

                                           ATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCC                                                    >  SRR4302537.1977199/1‑94 (MQ=60)

                                             CTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAG                                                  >  SRR4302537.2895079/1‑94 (MQ=60)

                                             CTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAG                                                  <  SRR4302537.1626939/94‑1 (MQ=60)

                                              TCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGG                                                 <  SRR4302537.2050092/94‑1 (MQ=60)

                                              TCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGG                                                 >  SRR4302537.1437664/1‑94 (MQ=60)

                                              TCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGG                                                 <  SRR4302537.4875296/94‑1 (MQ=60)

                                              TCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGG                                                 <  SRR4302537.3018975/94‑1 (MQ=60)

                                               CCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGG                                                <  SRR4302537.459962/94‑1 (MQ=60)

                                               CCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGG                                                >  SRR4302537.2866833/1‑94 (MQ=60)

                                               CCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGG                                                <  SRR4302537.5935354/94‑1 (MQ=60)

                                                CCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGT                                               >  SRR4302537.2587126/1‑94 (MQ=60)

                                                CCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGT                                               >  SRR4302537.3109860/1‑94 (MQ=60)

                                                CCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGT                                               <  SRR4302537.5834471/94‑1 (MQ=60)

                                                 CGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTG                                              <  SRR4302537.703460/94‑1 (MQ=60)

                                                 CGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTG                                              >  SRR4302537.1886321/1‑94 (MQ=60)

                                                 CGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTG                                              <  SRR4302537.951397/94‑1 (MQ=60)

                                                  GTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGT                                             >  SRR4302537.4708082/1‑94 (MQ=60)

                                                  GTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGT                                             >  SRR4302537.4655052/1‑94 (MQ=60)

                                                  GTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGT                                             >  SRR4302537.2543330/1‑94 (MQ=60)

                                                  GTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGT                                             <  SRR4302537.880825/94‑1 (MQ=60)

                                                   TCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTA                                            <  SRR4302537.3102034/94‑1 (MQ=60)

                                                    CTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTAC                                           >  SRR4302537.1964427/1‑94 (MQ=60)

                                                          CATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTT                                     <  SRR4302537.2221593/94‑1 (MQ=60)

                                                          CATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTT                                     >  SRR4302537.1250671/1‑94 (MQ=60)

                                                           ATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTT                                    >  SRR4302537.5378623/1‑94 (MQ=60)

                                                           ATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTT                                    >  SRR4302537.1635209/1‑94 (MQ=60)

                                                           ATACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTT                                    >  SRR4302537.6190611/1‑94 (MQ=60)

                                                             ACCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTA                                  <  SRR4302537.3854783/94‑1 (MQ=60)

                                                              CCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTAT                                 <  SRR4302537.5055501/94‑1 (MQ=60)

                                                              CCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTAT                                 >  SRR4302537.89270/1‑94 (MQ=60)

                                                              CCGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTAT                                 <  SRR4302537.3009275/94‑1 (MQ=60)

                                                               CGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATG                                >  SRR4302537.5759082/1‑94 (MQ=60)

                                                               CGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATG                                <  SRR4302537.2383280/94‑1 (MQ=60)

                                                               CGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATG                                <  SRR4302537.3034581/94‑1 (MQ=60)

                                                               CGATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATG                                <  SRR4302537.3467742/94‑1 (MQ=60)

                                                                GATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGC                               <  SRR4302537.2236208/94‑1 (MQ=60)

                                                                GATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGC                               <  SRR4302537.827571/94‑1 (MQ=60)

                                                                GATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGC                               >  SRR4302537.4094902/1‑94 (MQ=60)

                                                                GATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGC                               >  SRR4302537.3376675/1‑94 (MQ=60)

                                                                GATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGA                               >  SRR4302537.5286136/1‑94 (MQ=60)

                                                                 ATACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCA                              >  SRR4302537.5747508/1‑94 (MQ=60)

                                                                   ACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGG                            <  SRR4302537.5915694/94‑1 (MQ=60)

                                                                   ACGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGG                            <  SRR4302537.5732590/94‑1 (MQ=60)

                                                                    CGCTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGT                           >  SRR4302537.303912/1‑94 (MQ=60)

                                                                      CTGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGC                         >  SRR4302537.4753176/1‑94 (MQ=60)

                                                                       TGGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCT                        <  SRR4302537.6106971/94‑1 (MQ=60)

                                                                        GGTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTG                       <  SRR4302537.1422542/94‑1 (MQ=60)

                                                                         GTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGG                      <  SRR4302537.6379958/94‑1 (MQ=60)

                                                                         GTGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGGACAACGCGTATCGATATGGCATTAAACCAGGGAGTACCTTCTTTTATGCAGGTGCTGG                      >  SRR4302537.1253208/1‑94 (MQ=60)

                                                                          TGGTGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGG                     <  SRR4302537.4674000/94‑1 (MQ=60)

                                                                             TGTGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGT                  <  SRR4302537.29082/94‑1 (MQ=60)

                                                                               TGTGATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCG                <  SRR4302537.4391064/94‑1 (MQ=60)

                                                                                  GATGCTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCA             >  SRR4302537.2684813/1‑94 (MQ=60)

                                                                                      CTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAA         >  SRR4302537.3108336/1‑94 (MQ=60)

                                                                                      CTGGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAA         >  SRR4302537.5929369/1‑94 (MQ=60)

                                                                                        GGTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATG       >  SRR4302537.185373/1‑94 (MQ=60)

                                                                                         GTTTGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGA      <  SRR4302537.6384548/94‑1 (MQ=60)

                                                                                            TGCCCATCCCCAAAAGTACAACGCGTATCGATATGTCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAAT   <  SRR4302537.2637793/94‑1 (MQ=60)

                                                                                            TGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAAT   <  SRR4302537.865952/94‑1 (MQ=60)

                                                                                            TGCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAAT   <  SRR4302537.839381/94‑1 (MQ=60)

                                                                                             GCCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATG  <  SRR4302537.6055183/94‑1 (MQ=60)

AATGAAAAAAGGCACCGTTCTTAATTCTGATATTTCATCGGTGATCTCCCGTCTGGGACATACCGATACGCTGGTGGTGTGTGATGCTGGTTTACCCATCCCCAAAAGTACAACGCGTATCGATATGGCATTAACCCAGGGTGTACCTTCTTTTATGCAGGTGCTGGGCGTCGTCACAAATGAAATG  >  NC_000913/3930976‑3931162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 14 ≤` `ATCG/ATCG` `< 28 ≤` `ATCG/ATCG` `< 34 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`