Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 882 254.4 12957588 98.0% 12698436 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,557 2 bp→GC coding (451‑452/930 nt) ilvE → branched‑chain amino‑acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,5570CG99.4% 621.6 / NA 180R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (82/97);  minor base T (1/0);  total (83/97)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,950,5580GC99.4% 617.5 / NA 180R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (82/97);  minor base T (1/0);  total (83/97)
Fisher's exact test for biased strand distribution p-value = 4.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 189 total aligned reads displayed.

ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGT  >  NC_000913/3950470‑3950643
                                                                                       ||                                                                                     
aTCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCa                                                                                   >  2:5978548/1‑93 (MQ=255)
aTCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCa                                                                                   >  1:2788533/1‑93 (MQ=255)
    ctgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAaca                                                                               >  1:4424359/1‑93 (MQ=255)
     tgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac                                                                              >  1:2406669/1‑93 (MQ=255)
     tgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac                                                                              <  2:41618/93‑1 (MQ=255)
      gctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGTGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAcc                                                                             >  1:1388768/1‑93 (MQ=255)
        tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt                                                                           <  1:557666/93‑1 (MQ=255)
        tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt                                                                           <  2:5654274/93‑1 (MQ=255)
        tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt                                                                           >  1:2585870/1‑93 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                         <  1:4961677/93‑1 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                         <  1:835039/93‑1 (MQ=255)
           cccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATccc                                                                        >  1:393727/1‑93 (MQ=255)
           cccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATccc                                                                        <  2:3272477/93‑1 (MQ=255)
            ccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg                                                                       >  1:2085300/1‑93 (MQ=255)
             cGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGa                                                                      >  1:1908325/1‑93 (MQ=255)
             cGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGa                                                                      >  1:2847386/1‑93 (MQ=255)
             cGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGa                                                                      <  1:3980729/93‑1 (MQ=255)
              gTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc                                                                     <  2:4230515/93‑1 (MQ=255)
              gTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc                                                                     <  1:3840837/93‑1 (MQ=255)
              gTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc                                                                     <  2:3245469/93‑1 (MQ=255)
              gTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAACAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc                                                                     >  2:5838749/1‑93 (MQ=255)
                 gggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggc                                                                  <  2:1477434/93‑1 (MQ=255)
                  ggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggcg                                                                 >  2:495630/1‑93 (MQ=255)
                    aGCGAATCTGGGCGCAGAAGCGCTGGAGCAGGGGCTCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACggcggc                                                               >  2:1259885/1‑93 (MQ=255)
                     gCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa                                                              <  2:1406453/93‑1 (MQ=255)
                       gTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa                                                            >  1:3157293/1‑93 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATTGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                           >  1:6168907/1‑93 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                           <  2:3490487/93‑1 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                           <  1:1621252/93‑1 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                           <  1:4871747/93‑1 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                           <  2:629467/93‑1 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                         <  2:1451833/93‑1 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                         <  2:3654569/93‑1 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                         <  1:6474527/93‑1 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                         <  1:611319/93‑1 (MQ=255)
                           cTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGcc                                                        <  1:5805839/93‑1 (MQ=255)
                            tGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg                                                       >  1:189457/1‑93 (MQ=255)
                             gggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCgg                                                      <  2:540472/93‑1 (MQ=255)
                             gggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCgg                                                      >  1:777219/1‑93 (MQ=255)
                             gggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCgg                                                      <  1:5431514/93‑1 (MQ=255)
                               gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtg                                                    <  2:3009044/93‑1 (MQ=255)
                                cgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtgg                                                   <  1:1488674/93‑1 (MQ=255)
                                  cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTa                                                 <  2:297211/93‑1 (MQ=255)
                                   aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTaa                                                <  1:1081765/93‑1 (MQ=255)
                                   aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTaa                                                >  1:2760220/1‑93 (MQ=255)
                                   aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGACggtcgtga                                                >  2:1214188/1‑91 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                               >  2:2706580/1‑93 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                               >  2:3587312/1‑93 (MQ=255)
                                        cgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAcc                                           <  1:4338296/93‑1 (MQ=255)
                                          cTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctc                                         <  2:5912337/93‑1 (MQ=255)
                                           tGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctct                                        >  2:2079754/1‑93 (MQ=255)
                                            ggAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctc                                       <  1:4486894/93‑1 (MQ=255)
                                             gAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctct                                      <  1:1911499/93‑1 (MQ=255)
                                              aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCtt                                     >  2:1899146/1‑93 (MQ=255)
                                               gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc                                    >  2:1174859/1‑93 (MQ=255)
                                                cAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTcc                                   >  2:5597528/1‑93 (MQ=255)
                                                 aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTccc                                  >  2:1041100/1‑93 (MQ=255)
                                                 aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTccc                                  >  1:5160974/1‑93 (MQ=255)
                                                 aGGGGAGCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGCACCTACCTCTCTTccc                                  >  2:4373874/1‑93 (MQ=255)
                                                    ggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc                               >  2:4409801/1‑93 (MQ=255)
                                                    ggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc                               >  2:5757534/1‑93 (MQ=255)
                                                     gATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct                              >  2:3133766/1‑93 (MQ=255)
                                                     gATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct                              >  1:3735864/1‑93 (MQ=255)
                                                     gATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct                              <  2:385991/93‑1 (MQ=255)
                                                     gATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCCTCCctgct                              <  2:6324493/93‑1 (MQ=255)
                                                       tCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtgg                            <  2:5593865/93‑1 (MQ=255)
                                                       tCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtgg                            >  1:968732/1‑93 (MQ=255)
                                                         gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                          <  2:4451083/93‑1 (MQ=255)
                                                           tgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTggg                        >  1:4707700/1‑93 (MQ=255)
                                                            gcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt                       <  2:5037552/93‑1 (MQ=255)
                                                             cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                      <  1:4180511/93‑1 (MQ=255)
                                                             cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                      >  2:2855165/1‑93 (MQ=255)
                                                             cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                      <  1:1072772/93‑1 (MQ=255)
                                                             cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                      <  2:137982/93‑1 (MQ=255)
                                                              gatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAg                     >  1:4772526/1‑93 (MQ=255)
                                                               gtgGTTTCCTCCTGGAACCGCGCAGCACCAAGCACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                    <  1:5719412/92‑1 (MQ=255)
                                                               atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                    <  2:922818/93‑1 (MQ=255)
                                                                 ggTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGa                  <  2:4366803/93‑1 (MQ=255)
                                                                  gTTTCTTCCTGGAACCGGGCACCACCAAACCCCCTCCCGACGGCGGCAAAAGCCGGTGGTAACTGCCTCTCTTCCCTGCTGGTGGGTAGCGaa                 <  2:5138670/93‑1 (MQ=255)
                                                                  gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                 <  1:4045639/93‑1 (MQ=255)
                                                                  gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                 >  2:2154718/1‑93 (MQ=255)
                                                                  gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                 >  1:6303886/1‑93 (MQ=255)
                                                                   tttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAg                >  1:2004898/1‑93 (MQ=255)
                                                                   tttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAg                <  2:4026079/93‑1 (MQ=255)
                                                                   tttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAg                >  2:2918625/1‑93 (MQ=255)
                                                                    tgcctcctGGAACCGCGCAGCACCAACCACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgc               <  2:4645861/91‑1 (MQ=255)
                                                                    tgcctcctGGAACCGCGCAGCACCAAACACCAGCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgc               <  1:947946/91‑1 (MQ=255)
                                                                     tcctccTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcg              <  1:3915883/93‑1 (MQ=255)
                                                                      cctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgc             >  1:131691/1‑93 (MQ=255)
                                                                        tcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgcgc           <  1:4286045/93‑1 (MQ=255)
                                                                          ctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccg         >  1:6286393/1‑93 (MQ=255)
                                                                           tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCCAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccgc        >  2:2406496/1‑93 (MQ=255)
                                                                           tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccgc        >  1:6207964/1‑93 (MQ=255)
                                                                           tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccgc        >  2:1780057/1‑93 (MQ=255)
                                                                             gAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa      <  1:2184720/93‑1 (MQ=255)
                                                                             gAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa      <  1:6421606/93‑1 (MQ=255)
                                                                                ccGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAGTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACgg   >  1:3615319/1‑93 (MQ=255)
                                                                                ccGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTTCCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACgg   <  1:1726147/93‑1 (MQ=255)
                                                                                ccGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACgg   <  1:1160942/93‑1 (MQ=255)
                                                                                 cgcgCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGt  <  1:166790/93‑1 (MQ=255)
                                                                                       ||                                                                                     
ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGT  >  NC_000913/3950470‑3950643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  NC_000913/3950469‑3950646
                                                                                        |                                                                                         
TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCA                                                                                      >  SRR4302537.2430932/1‑94 (MQ=60)
TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCA                                                                                      >  SRR4302537.2808032/1‑94 (MQ=60)
   CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   >  SRR4302537.6167242/1‑94 (MQ=60)
   CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   >  SRR4302537.1171501/1‑94 (MQ=60)
    GCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACA                                                                                  >  SRR4302537.4454914/1‑94 (MQ=60)
    GCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACA                                                                                  <  SRR4302537.2028695/94‑1 (MQ=60)
     CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                                 >  SRR4302537.2349578/1‑94 (MQ=60)
     CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                                 >  SRR4302537.2423610/1‑94 (MQ=60)
      TGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGTGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACC                                                                                >  SRR4302537.1398451/1‑94 (MQ=60)
      TGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACC                                                                                >  SRR4302537.1395722/1‑94 (MQ=60)
       GCTTTCAGGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTGCCTCCTGGAACCGCGCAGCACCAAACACCA                                                                               <  SRR4302537.5686947/94‑1 (MQ=60)
        CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                              >  SRR4302537.2603963/1‑94 (MQ=60)
         TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                             <  SRR4302537.561507/94‑1 (MQ=60)
           TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           >  SRR4302537.396458/1‑94 (MQ=60)
           TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           <  SRR4302537.4995880/94‑1 (MQ=60)
           TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           <  SRR4302537.840752/94‑1 (MQ=60)
            CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                          >  SRR4302537.1923138/1‑94 (MQ=60)
            CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                          >  SRR4302537.2099999/1‑94 (MQ=60)
             CCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCTTCCTGGAACCGCGCAGCACCAAACACCATCCCGA                                                                         >  SRR4302537.2179267/1‑94 (MQ=60)
             CCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGA                                                                         >  SRR4302537.2867319/1‑94 (MQ=60)
             CCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGA                                                                         >  SRR4302537.1921575/1‑94 (MQ=60)
              CGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                        <  SRR4302537.4008596/94‑1 (MQ=60)
              CGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                        <  SRR4302537.4008595/94‑1 (MQ=60)
               GTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                       <  SRR4302537.5823570/94‑1 (MQ=60)
               GTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                       <  SRR4302537.3867741/94‑1 (MQ=60)
               GTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                       <  SRR4302537.4498754/94‑1 (MQ=60)
                 GGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGC                                                                     <  SRR4302537.648306/94‑1 (MQ=60)
                 GGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGC                                                                     <  SRR4302537.2803110/94‑1 (MQ=60)
                       CGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAA                                                               >  SRR4302537.3179455/1‑94 (MQ=60)
                        GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATTGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                              >  SRR4302537.6211387/1‑94 (MQ=60)
                        GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                              >  SRR4302537.6234826/1‑94 (MQ=60)
                         TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                             <  SRR4302537.4774420/94‑1 (MQ=60)
                         TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                             >  SRR4302537.836151/1‑94 (MQ=60)
                         TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                             <  SRR4302537.1467680/94‑1 (MQ=60)
                         TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                             <  SRR4302537.4905370/94‑1 (MQ=60)
                         TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                             <  SRR4302537.1632588/94‑1 (MQ=60)
                           TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                           <  SRR4302537.6518473/94‑1 (MQ=60)
                           TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                           <  SRR4302537.615493/94‑1 (MQ=60)
                            CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                          <  SRR4302537.5845649/94‑1 (MQ=60)
                            CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                          >  SRR4302537.190805/1‑94 (MQ=60)
                             TGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGG                                                         <  SRR4302537.4969774/94‑1 (MQ=60)
                             TGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGG                                                         >  SRR4302537.782550/1‑94 (MQ=60)
                              GGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGT                                                        <  SRR4302537.5468559/94‑1 (MQ=60)
                               GGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTG                                                       <  SRR4302537.3960397/94‑1 (MQ=60)
                                 CGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGT                                                     <  SRR4302537.1499034/94‑1 (MQ=60)
                                   CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAA                                                   >  SRR4302537.2779481/1‑94 (MQ=60)
                                    AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  <  SRR4302537.1089233/94‑1 (MQ=60)
                                         CGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCT                                             <  SRR4302537.4368405/94‑1 (MQ=60)
                                          GCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTC                                            <  SRR4302537.6427749/94‑1 (MQ=60)
                                           CTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCT                                           <  SRR4302537.3130445/94‑1 (MQ=60)
                                           CTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCT                                           <  SRR4302537.6154985/94‑1 (MQ=60)
                                             GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                         <  SRR4302537.19453/94‑1 (MQ=60)
                                             GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                         <  SRR4302537.4517933/94‑1 (MQ=60)
                                              GAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTT                                        <  SRR4302537.1924769/94‑1 (MQ=60)
                                                GCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCC                                      >  SRR4302537.100298/1‑94 (MQ=60)
                                                 CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                     >  SRR4302537.5196556/1‑94 (MQ=60)
                                                 CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                     >  SRR4302537.5479153/1‑94 (MQ=60)
                                                  AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCAGGTGGTAACTACCTCTCTTCCCT                                    >  SRR4302537.931023/1‑94 (MQ=60)
                                                    GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                  >  SRR4302537.6281520/1‑94 (MQ=60)
                                                     GGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCT                                 >  SRR4302537.3762102/1‑94 (MQ=60)
                                                      GATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTG                                <  SRR4302537.1861732/94‑1 (MQ=60)
                                                       ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                               >  SRR4302537.975411/1‑94 (MQ=60)
                                                         CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302537.1597778/1‑94 (MQ=60)
                                                          GATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGG                            >  SRR4302537.4367978/1‑94 (MQ=60)
                                                          GATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGG                            <  SRR4302537.1679037/94‑1 (MQ=60)
                                                           ATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGG                           >  SRR4302537.4740271/1‑94 (MQ=60)
                                                            TGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGT                          >  SRR4302537.4307274/1‑94 (MQ=60)
                                                              CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        >  SRR4302537.4805479/1‑94 (MQ=60)
                                                              CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        <  SRR4302537.1080165/94‑1 (MQ=60)
                                                              CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        <  SRR4302537.4209691/94‑1 (MQ=60)
                                                              CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        <  SRR4302537.3652113/94‑1 (MQ=60)
                                                              CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        >  SRR4302537.5425888/1‑94 (MQ=60)
                                                              CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        <  SRR4302537.687501/94‑1 (MQ=60)
                                                               GATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGC                       >  SRR4302537.4413782/1‑94 (MQ=60)
                                                                GTGGTTTCCTCCTGGAACCGCGCAGCACCAAGCACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                      <  SRR4302537.5758557/94‑1 (MQ=60)
                                                                  GGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAA                    >  SRR4302537.6347053/1‑94 (MQ=60)
                                                                   GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                   >  SRR4302537.2018837/1‑94 (MQ=60)
                                                                   GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                   >  SRR4302537.3833127/1‑94 (MQ=60)
                                                                   GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                   <  SRR4302537.4073927/94‑1 (MQ=60)
                                                                   GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                   <  SRR4302537.4869327/94‑1 (MQ=60)
                                                                   GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCCCTCTTCCCTGCTGGTGGGTAGCGAAG                   <  SRR4302537.113786/94‑1 (MQ=60)
                                                                     TTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGTCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCG                 <  SRR4302537.4577049/94‑1 (MQ=60)
                                                                     TGCCTCCTGGAACCGCGCAGCACCAAACACCAGCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCG                 <  SRR4302537.954503/94‑1 (MQ=60)
                                                                      TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC                <  SRR4302537.2654706/94‑1 (MQ=60)
                                                                      TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC                >  SRR4302537.132624/1‑94 (MQ=60)
                                                                      TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC                >  SRR4302537.169803/1‑94 (MQ=60)
                                                                      TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC                <  SRR4302537.3943254/94‑1 (MQ=60)
                                                                      TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC                <  SRR4302537.3943253/94‑1 (MQ=60)
                                                                       CCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCG               >  SRR4302537.4029814/1‑94 (MQ=60)
                                                                        CTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGC              >  SRR4302537.3584420/1‑94 (MQ=60)
                                                                         TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC             <  SRR4302537.4315835/94‑1 (MQ=60)
                                                                          CCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCG            >  SRR4302537.6329490/1‑94 (MQ=60)
                                                                          CCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCG            >  SRR4302537.966745/1‑94 (MQ=60)
                                                                           CTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGC           >  SRR4302537.6250737/1‑94 (MQ=60)
                                                                              GAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAC        <  SRR4302537.6465255/94‑1 (MQ=60)
                                                                              GAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAC        <  SRR4302537.2200115/94‑1 (MQ=60)
                                                                                ACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAGTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGG      >  SRR4302537.3640807/1‑94 (MQ=60)
                                                                                 CCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTTCCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGT     <  SRR4302537.1738209/94‑1 (MQ=60)
                                                                                 CCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGT     <  SRR4302537.1169016/94‑1 (MQ=60)
                                                                                  CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT    <  SRR4302537.167982/94‑1 (MQ=60)
                                                                                    CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  SRR4302537.1153993/1‑94 (MQ=60)
                                                                                    CGCAGCACCAAACACCATCCCGACGGCGGAAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  <  SRR4302537.5152308/94‑1 (MQ=60)
                                                                                        |                                                                                         
TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  NC_000913/3950469‑3950646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑