Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,250,165 C→T R74R (CGC→CGT)  yhaH → putative cytochrome

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,250,1650CT98.8% 879.6 / ‑2.9 248R74R (CGC→CGT) yhaHputative cytochrome
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (77/167);  total (78/170)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.64e-01

Only 100 of 271 total aligned reads displayed.

AGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCA  >  NC_000913/3250075‑3250254
                                                                                          |                                                                                         
aGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCt                                                                                         <  1:8587008/93‑1 (MQ=255)
aGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCt                                                                                         <  2:5378307/93‑1 (MQ=255)
aGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCt                                                                                         >  2:1825851/1‑93 (MQ=255)
aGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCt                                                                                         >  2:2278977/1‑93 (MQ=255)
 ggCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTg                                                                                        <  2:3791631/93‑1 (MQ=255)
   cTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCa                                                                                      >  2:7955925/1‑93 (MQ=255)
     ggCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACg                                                                                    >  1:4923297/1‑93 (MQ=255)
        aaCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGaca                                                                                 >  2:363363/1‑93 (MQ=255)
        aaCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGaca                                                                                 <  2:4672713/93‑1 (MQ=255)
        aaCGCGCTGGAGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGGGGGCGGGTCAGTTCCGCCGTCTGCACGaca                                                                                 >  1:4456201/1‑93 (MQ=255)
         aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGacac                                                                                <  2:2873141/93‑1 (MQ=255)
         aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGacac                                                                                >  1:7465941/1‑93 (MQ=255)
         aCGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGacac                                                                                <  2:4040129/93‑1 (MQ=255)
          cgcgCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACacc                                                                               >  2:2077497/1‑93 (MQ=255)
           gcgcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACaccg                                                                              <  2:4233604/93‑1 (MQ=255)
            cgcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACaccga                                                                             >  2:576962/1‑93 (MQ=255)
             gcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACaccgac                                                                            <  2:1767612/93‑1 (MQ=255)
             gcTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACaccgac                                                                            <  1:175464/93‑1 (MQ=255)
              cTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACaccgacc                                                                           >  1:687899/1‑93 (MQ=255)
              cTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACaccgacc                                                                           >  1:3242028/1‑93 (MQ=255)
                ggtggCGAAGGCATCCTGACGACCATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGc                                                                         <  2:5938493/93‑1 (MQ=255)
                ggtggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGc                                                                         >  1:7650177/1‑93 (MQ=255)
                ggtggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGc                                                                         >  1:6248788/1‑93 (MQ=255)
                 gtggCGAAGGCATCCTGACGACACTCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCt                                                                        <  2:4757130/93‑1 (MQ=255)
                 gtggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCt                                                                        <  1:5565122/93‑1 (MQ=255)
                  tggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTc                                                                       <  2:7690589/93‑1 (MQ=255)
                  tggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTc                                                                       <  2:5138757/93‑1 (MQ=255)
                  tggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTc                                                                       <  1:1908000/93‑1 (MQ=255)
                   ggCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCg                                                                      >  1:3651587/1‑93 (MQ=255)
                    gCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCgg                                                                     >  1:945174/1‑93 (MQ=255)
                    gCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCgg                                                                     <  2:3124991/93‑1 (MQ=255)
                    gCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGCGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCgg                                                                     >  1:1268714/1‑93 (MQ=255)
                     cGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGc                                                                    <  2:8393216/93‑1 (MQ=255)
                       aaGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgt                                                                  <  1:4815312/93‑1 (MQ=255)
                       aaGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCACTCGGCgt                                                                  >  2:3440263/1‑93 (MQ=255)
                         ggCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtgg                                                                >  2:6304699/1‑93 (MQ=255)
                          gCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtggt                                                               >  1:538693/1‑93 (MQ=255)
                          gCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtggt                                                               <  1:5979780/93‑1 (MQ=255)
                            aTCCTGACGACAATCTACGGTCTTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGtggtgg                                                             >  1:8235110/1‑93 (MQ=255)
                              ccTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGc                                                           <  2:1394868/93‑1 (MQ=255)
                               cTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCa                                                          <  1:3141836/93‑1 (MQ=255)
                               cTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCa                                                          <  1:1570237/93‑1 (MQ=255)
                                 gacgacAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACt                                                        <  2:79251/93‑1 (MQ=255)
                                    gacAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCt                                                     >  1:4736159/1‑93 (MQ=255)
                                     acAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTa                                                    <  2:1349807/93‑1 (MQ=255)
                                      cAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAt                                                   >  2:758056/1‑93 (MQ=255)
                                      cAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAt                                                   <  2:2820997/93‑1 (MQ=255)
                                       aaTCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAtt                                                  <  1:703313/93‑1 (MQ=255)
                                         tCTACGTTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCt                                                <  1:3555863/93‑1 (MQ=255)
                                           tACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCAGACGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTa                                              >  2:7062508/1‑93 (MQ=255)
                                            aCGGTAGTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTaa                                             <  1:7221220/93‑1 (MQ=255)
                                              ggTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATc                                           >  1:5540906/1‑93 (MQ=255)
                                              cgTATTCTGGTGTTTTTTCCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATc                                           <  2:1150704/92‑1 (MQ=255)
                                               gTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATcc                                          <  2:4983220/93‑1 (MQ=255)
                                               gTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATcc                                          >  1:4175463/1‑93 (MQ=255)
                                                  ttCTGGTGTTTTTTCCGTGGTGGGCGGTTCAGTTCCTCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGt                                       <  2:7449950/93‑1 (MQ=255)
                                                  ttCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGt                                       <  1:1661026/93‑1 (MQ=255)
                                                    cTGGTTTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGttt                                     <  2:5991786/93‑1 (MQ=255)
                                                    cTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGttt                                     <  1:6935463/93‑1 (MQ=255)
                                                       gtgtTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATc                                  >  1:1882562/1‑93 (MQ=255)
                                                        tgtTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                 <  2:262509/93‑1 (MQ=255)
                                                        tgtTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCg                                 <  1:629305/93‑1 (MQ=255)
                                                          tttttACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtggt                                                               <  2:2708325/61‑1 (MQ=255)
                                                          tttttACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCgtggt                                                               >  1:2708325/1‑61 (MQ=255)
                                                          tttttACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggc                               <  1:7638787/93‑1 (MQ=255)
                                                          tttttACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggc                               <  2:7032785/93‑1 (MQ=255)
                                                           ttttACCGTGGTGGGCGGTTCAGTTCCGCCGGCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggct                              <  2:6603442/93‑1 (MQ=255)
                                                            tttACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctg                             <  2:8350247/93‑1 (MQ=255)
                                                             ttACCGCGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCggctgg                            <  1:6742403/93‑1 (MQ=255)
                                                                ccGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtt                         >  2:900865/1‑93 (MQ=255)
                                                                 cGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCtta                        <  2:1119583/93‑1 (MQ=255)
                                                                  gtggtgGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCTCTGCTATTCTTAATCCCGTTTATCGGCTGGCttat                       <  2:8719026/93‑1 (MQ=255)
                                                                  gtggtgGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttat                       <  1:3486177/93‑1 (MQ=255)
                                                                  gtggtgGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttat                       <  2:6030694/93‑1 (MQ=255)
                                                                   tggtggGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatt                      <  1:1825851/93‑1 (MQ=255)
                                                                   tggtggGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatt                      <  1:2278977/93‑1 (MQ=255)
                                                                   aggtggGCGGTTCAGTTCCGCCGTATGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatt                      <  1:3813822/92‑1 (MQ=255)
                                                                    ggtggGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCttatta                     <  1:4370961/93‑1 (MQ=255)
                                                                     gtggGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTtattat                    <  2:6971558/93‑1 (MQ=255)
                                                                     gtggGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTtattat                    <  1:8776056/93‑1 (MQ=255)
                                                                      tggGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatc                   <  1:1348436/93‑1 (MQ=255)
                                                                      ggggCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatc                   <  1:7661677/92‑1 (MQ=255)
                                                                       gggCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatca                  <  1:2470984/93‑1 (MQ=255)
                                                                       gggCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTatca                  <  1:7918334/93‑1 (MQ=255)
                                                                            gTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGtt             >  2:1711812/1‑92 (MQ=255)
                                                                            gTTCAGTGCCGCCGTCTGCCTGCCACCGCCCGCTCGGCTTGTTTGGCACTTCTATTCTTAATCTCGTTTATCGATTGGCTTATTATCATCGTc             <  2:5503556/93‑1 (MQ=255)
                                                                             ttCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCt            >  1:7365331/1‑93 (MQ=255)
                                                                              tCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCtt           <  1:5863938/93‑1 (MQ=255)
                                                                                aGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTa         <  2:6205734/93‑1 (MQ=255)
                                                                                 gTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTaa        <  2:3297754/93‑1 (MQ=255)
                                                                                 gTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTaa        >  1:2972966/1‑93 (MQ=255)
                                                                                  ttCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAc       <  1:5143969/93‑1 (MQ=255)
                                                                                   tCCGCCGTCTTCACGACACCGACCGGTCGGCGGGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACt      <  2:5537509/93‑1 (MQ=255)
                                                                                   tCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACt      <  2:7415611/93‑1 (MQ=255)
                                                                                   tCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACt      <  2:2470271/93‑1 (MQ=255)
                                                                                     cgccgtCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGc    <  2:1570190/93‑1 (MQ=255)
                                                                                      gccgtcTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGcc   <  1:4018905/93‑1 (MQ=255)
                                                                                      gccgtcTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGcc   <  1:6484373/93‑1 (MQ=255)
                                                                                      gccgtcTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGcc   <  2:6176878/93‑1 (MQ=255)
                                                                                       ccgtcTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCa  <  2:8072837/93‑1 (MQ=255)
                                                                                          |                                                                                         
AGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCA  >  NC_000913/3250075‑3250254

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCG  >  NC_000913/3250072‑3250258
                                                                                             |                                                                                             
GTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGT                                                                                               >  SRR4302541.8249870/1‑94 (MQ=60)
GTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGT                                                                                               >  SRR4302541.3387052/1‑94 (MQ=60)
 TTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTC                                                                                              <  SRR4302541.3991040/94‑1 (MQ=60)
  TAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCT                                                                                             <  SRR4302541.5881651/94‑1 (MQ=60)
   AGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTG                                                                                            <  SRR4302541.8679180/94‑1 (MQ=60)
   AGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTG                                                                                            <  SRR4302541.8679179/94‑1 (MQ=60)
    GGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTCGTGTTTTTACCGTGGTGGGCGGTTCAGCTCCGCCGTCTGC                                                                                           <  SRR4302541.488803/94‑1 (MQ=60)
     GCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCA                                                                                          >  SRR4302541.7462457/1‑94 (MQ=60)
     GCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCA                                                                                          <  SRR4302541.6809131/94‑1 (MQ=60)
      CTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCAC                                                                                         <  SRR4302541.6269623/94‑1 (MQ=60)
       TGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACG                                                                                        <  SRR4302541.1904265/94‑1 (MQ=60)
       TGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACG                                                                                        >  SRR4302541.437060/1‑94 (MQ=60)
       TGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACG                                                                                        >  SRR4302541.4973531/1‑94 (MQ=60)
         GCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGAC                                                                                      <  SRR4302541.312407/94‑1 (MQ=60)
          CAACGCGCTGGAGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGGGGGCGGGTCAGTTCCGCCGTCTGCACGACA                                                                                     >  SRR4302541.4501048/1‑94 (MQ=60)
           AACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACAC                                                                                    >  SRR4302541.7545740/1‑94 (MQ=60)
           AACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACAC                                                                                    >  SRR4302541.1633775/1‑94 (MQ=60)
            ACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACC                                                                                   <  SRR4302541.565/94‑1 (MQ=60)
               CGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGAC                                                                                >  SRR4302541.2720224/1‑94 (MQ=60)
               CGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGAC                                                                                >  SRR4302541.2334273/1‑94 (MQ=60)
                GCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACC                                                                               <  SRR4302541.176789/94‑1 (MQ=60)
                GCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACC                                                                               >  SRR4302541.3274753/1‑94 (MQ=60)
                GCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACC                                                                               >  SRR4302541.694375/1‑94 (MQ=60)
                 CTGGTGGCGAATGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCG                                                                              <  SRR4302541.6953576/94‑1 (MQ=60)
                 CTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCG                                                                              <  SRR4302541.7327616/94‑1 (MQ=60)
                 CTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCG                                                                              <  SRR4302541.770491/94‑1 (MQ=60)
                 CTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCG                                                                              >  SRR4302541.6448165/1‑94 (MQ=60)
                  TGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGC                                                                             >  SRR4302541.6312624/1‑94 (MQ=60)
                  TGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGC                                                                             >  SRR4302541.7731684/1‑94 (MQ=60)
                   GGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCT                                                                            <  SRR4302541.6878094/94‑1 (MQ=60)
                    GTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTC                                                                           <  SRR4302541.5622083/94‑1 (MQ=60)
                    GTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTC                                                                           <  SRR4302541.6315697/94‑1 (MQ=60)
                     TGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCG                                                                          <  SRR4302541.1926339/94‑1 (MQ=60)
                     TGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCG                                                                          <  SRR4302541.4027087/94‑1 (MQ=60)
                     TGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCG                                                                          <  SRR4302541.3791164/94‑1 (MQ=60)
                     TGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCG                                                                          >  SRR4302541.3688630/1‑94 (MQ=60)
                      GGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGG                                                                         >  SRR4302541.954166/1‑94 (MQ=60)
                      GGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGCGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGG                                                                         >  SRR4302541.1281334/1‑94 (MQ=60)
                       GCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGC                                                                        <  SRR4302541.8398450/94‑1 (MQ=60)
                       GCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGC                                                                        <  SRR4302541.4430250/94‑1 (MQ=60)
                        CGAAGGCGTCCTGACGACCATCTACGGTCATCTAAAGTTTTTCCCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCAACCGCTCGGCG                                                                       <  SRR4302541.7932499/94‑1 (MQ=42)
                        CGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCG                                                                       <  SRR4302541.8085669/94‑1 (MQ=60)
                        CGAAGGCATCCTGACGACAATCTACGGCATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCG                                                                       >  SRR4302541.7432300/1‑94 (MQ=60)
                          AAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTG                                                                     <  SRR4302541.4864407/94‑1 (MQ=60)
                            GGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGT                                                                   <  SRR4302541.1640397/94‑1 (MQ=60)
                            GGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGT                                                                   >  SRR4302541.543753/1‑94 (MQ=60)
                            GGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGG                                                                   >  SRR4302541.2747847/1‑94 (MQ=60)
                             GCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTG                                                                  <  SRR4302541.6040929/94‑1 (MQ=60)
                              CATCCTGACGACAATCTACGGTCTTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGG                                                                 >  SRR4302541.8324875/1‑94 (MQ=60)
                               ATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGG                                                                >  SRR4302541.6992560/1‑94 (MQ=60)
                               ATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCCGCACGACACCGACCGCTCGGCGTGGTGGG                                                                >  SRR4302541.4294274/1‑94 (MQ=60)
                                TCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGC                                                               <  SRR4302541.7444826/94‑1 (MQ=60)
                                 CCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGGGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCA                                                              <  SRR4302541.7092681/94‑1 (MQ=60)
                                  CTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCAC                                                             <  SRR4302541.3173571/94‑1 (MQ=60)
                                  CTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCAC                                                             <  SRR4302541.2493926/94‑1 (MQ=60)
                                  CTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCAC                                                             >  SRR4302541.5149711/1‑94 (MQ=60)
                                  CTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCAC                                                             <  SRR4302541.1585954/94‑1 (MQ=60)
                                  CTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCAC                                                             >  SRR4302541.5924036/1‑94 (MQ=60)
                                    GACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTG                                                           <  SRR4302541.7725058/94‑1 (MQ=60)
                                      CGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCT                                                         >  SRR4302541.4784502/1‑94 (MQ=60)
                                      CGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCT                                                         >  SRR4302541.4784503/1‑94 (MQ=60)
                                        ACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTAT                                                       <  SRR4302541.8916433/94‑1 (MQ=60)
                                          AATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTC                                                     <  SRR4302541.709930/94‑1 (MQ=60)
                                          AATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTC                                                     <  SRR4302541.1782263/94‑1 (MQ=60)
                                          AATATACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCGGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGTTGGGCACTGCTATTC                                                     <  SRR4302541.709931/94‑1 (MQ=60)
                                           ATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCT                                                    >  SRR4302541.7732875/1‑94 (MQ=60)
                                           ATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCT                                                    >  SRR4302541.1887857/1‑94 (MQ=60)
                                           ATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCT                                                    <  SRR4302541.4206368/94‑1 (MQ=60)
                                            TCTACGTTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTT                                                   <  SRR4302541.3591833/94‑1 (MQ=60)
                                            TCTACGGTATTCTGGTGTTTTTACCGTGGGGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTT                                                   <  SRR4302541.3591832/94‑1 (MQ=60)
                                              TACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAA                                                 <  SRR4302541.7787873/94‑1 (MQ=60)
                                               tttttttttttgttTTTTTAACGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAAT                                                <  SRR4302541.5625619/80‑1 (MQ=60)
                                               ACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAAT                                                <  SRR4302541.7734815/94‑1 (MQ=60)
                                               ACGGTAGTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAAT                                                <  SRR4302541.7298255/94‑1 (MQ=60)
                                                CGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATC                                               >  SRR4302541.5597644/1‑94 (MQ=60)
                                                 GGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCC                                              <  SRR4302541.5425330/94‑1 (MQ=60)
                                                 GGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCC                                              >  SRR4302541.4216874/1‑94 (MQ=60)
                                                  GTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCC                                             <  SRR4302541.8457640/94‑1 (MQ=60)
                                                  GTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCC                                             <  SRR4302541.906495/94‑1 (MQ=60)
                                                   TATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCG                                            <  SRR4302541.1304416/94‑1 (MQ=60)
                                                     TTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTT                                          <  SRR4302541.1677415/94‑1 (MQ=60)
                                                       CTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTA                                        <  SRR4302541.7009454/94‑1 (MQ=60)
                                                       CTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTA                                        <  SRR4302541.2043932/94‑1 (MQ=60)
                                                        TGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTAT                                       <  SRR4302541.7561944/94‑1 (MQ=60)
                                                         GGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATC                                      >  SRR4302541.1900649/1‑94 (MQ=60)
                                                          GTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCG                                     <  SRR4302541.6421063/94‑1 (MQ=60)
                                                          GTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCG                                     <  SRR4302541.3740000/94‑1 (MQ=60)
                                                          GTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCG                                     <  SRR4302541.199366/94‑1 (MQ=60)
                                                          GTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGATTATCG                                     <  SRR4302541.3181750/94‑1 (MQ=60)
                                                           TGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGG                                    <  SRR4302541.635222/94‑1 (MQ=60)
                                                           TGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGG                                    <  SRR4302541.6050526/94‑1 (MQ=60)
                                                            GTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGaatgtcaagatcggaagagcggttcagcagg                                   >  SRR4302541.2735279/1‑63 (MQ=60)
                                                            GTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGC                                   <  SRR4302541.6673734/94‑1 (MQ=60)
                                                             TTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCT                                  <  SRR4302541.7720242/94‑1 (MQ=60)
                                                              TTTTACCGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTG                                 <  SRR4302541.1705095/94‑1 (MQ=60)
                                                                TTACCGCGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGC                               <  SRR4302541.6814430/94‑1 (MQ=60)
                                                                    CGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTAT                           <  SRR4302541.8839590/94‑1 (MQ=60)
                                                                    CGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTAT                           <  SRR4302541.1828734/94‑1 (MQ=60)
                                                                    CGTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGAGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTAT                           <  SRR4302541.3234537/94‑1 (MQ=60)
                                                                    CGTGGGGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTAT                           <  SRR4302541.558748/94‑1 (MQ=60)
                                                                     GTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATT                          >  SRR4302541.1564284/1‑94 (MQ=60)
                                                                     GTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATT                          <  SRR4302541.3521083/94‑1 (MQ=60)
                                                                     GTGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATT                          <  SRR4302541.3521082/94‑1 (MQ=60)
                                                                      TGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTT                         >  SRR4302541.3642599/1‑94 (MQ=60)
                                                                      TGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTA                         <  SRR4302541.1843563/94‑1 (MQ=60)
                                                                      TGGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTA                         <  SRR4302541.2301012/94‑1 (MQ=60)
                                                                      AGGTGGGCGGTTCAGTTCCGCCGTATGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTA                         <  SRR4302541.3852235/94‑1 (MQ=60)
                                                                       GGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTAT                        <  SRR4302541.4066781/94‑1 (MQ=60)
                                                                       GGTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTAT                        <  SRR4302541.4414635/94‑1 (MQ=60)
                                                                        GTGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATC                       <  SRR4302541.8869853/94‑1 (MQ=60)
                                                                         TGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCA                      <  SRR4302541.1361768/94‑1 (MQ=60)
                                                                         GGGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCA                      <  SRR4302541.7743221/94‑1 (MQ=60)
                                                                          GGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCAT                     <  SRR4302541.3283366/94‑1 (MQ=60)
                                                                          GGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCAT                     <  SRR4302541.8005474/94‑1 (MQ=60)
                                                                          GGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCAT                     <  SRR4302541.4802124/94‑1 (MQ=60)
                                                                          GGGCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCAT                     <  SRR4302541.2495709/94‑1 (MQ=60)
                                                                            GCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCG                   <  SRR4302541.2654827/94‑1 (MQ=60)
                                                                            GCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCG                   <  SRR4302541.5637642/94‑1 (MQ=60)
                                                                            GCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCG                   <  SRR4302541.5610795/94‑1 (MQ=60)
                                                                            GCGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCG                   <  SRR4302541.7011359/94‑1 (MQ=60)
                                                                             CGGTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGT                  >  SRR4302541.36570/1‑94 (MQ=60)
                                                                               GTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCT                >  SRR4302541.7444050/1‑94 (MQ=60)
                                                                               GTTCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCT                <  SRR4302541.4709584/94‑1 (MQ=60)
                                                                                TTCAGTTCCGCCGGCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTT               <  SRR4302541.4804193/94‑1 (MQ=60)
                                                                                 TCAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTT              <  SRR4302541.5923588/94‑1 (MQ=60)
                                                                                  CAGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTA             <  SRR4302541.6021071/94‑1 (MQ=60)
                                                                                   AGTTCCGCCGTCTGCACGACCCCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTATTCCCGTTTATCGGCTGGCTTATTtttttcgtgtttac            >  SRR4302541.3002930/1‑80 (MQ=60)
                                                                                   AGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTTA            >  SRR4302541.5915298/1‑94 (MQ=60)
                                                                                   AGTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAA            >  SRR4302541.3002929/1‑94 (MQ=60)
                                                                                    GTTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAAC           <  SRR4302541.5355293/94‑1 (MQ=60)
                                                                                     TTCCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACT          <  SRR4302541.5196872/94‑1 (MQ=60)
                                                                                       CCGCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGC        <  SRR4302541.1484464/94‑1 (MQ=60)
                                                                                         GCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCA      <  SRR4302541.4058631/94‑1 (MQ=60)
                                                                                         GCCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCA      <  SRR4302541.6550468/94‑1 (MQ=60)
                                                                                          CCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATTATAGTCTTTTAATGCCAG     >  SRR4302541.16926/1‑94 (MQ=60)
                                                                                          CCGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAG     <  SRR4302541.4927192/94‑1 (MQ=60)
                                                                                           CGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGG    <  SRR4302541.3540483/94‑1 (MQ=60)
                                                                                           CGTCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGG    <  SRR4302541.7209130/94‑1 (MQ=60)
                                                                                             TCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCG  <  SRR4302541.139962/94‑1 (MQ=60)
                                                                                             TCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCG  <  SRR4302541.5235030/94‑1 (MQ=60)
                                                                                             TCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCG  <  SRR4302541.3339648/94‑1 (MQ=60)
                                                                                             |                                                                                             
GTTAGGCTGGCAACGCGCTGGTGGCGAAGGCATCCTGACGACAATCTACGGTATTCTGGTGTTTTTACCGTGGTGGGCGGTTCAGTTCCGCCGCCTGCACGACACCGACCGCTCGGCGTGGTGGGCACTGCTATTCTTAATCCCGTTTATCGGCTGGCTTATTATCATCGTCTTTAACTGCCAGGCG  >  NC_000913/3250072‑3250258

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑