Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,312,106 T→C Y517C (TAC→TGC)  infB ← protein chain initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,312,1060TC99.7% 1249.6 / ‑5.3 362Y517C (TAC→TGC) infBprotein chain initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (167/194);  total (168/194)
Fisher's exact test for biased strand distribution p-value = 4.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 370 total aligned reads displayed.

GCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCT  >  NC_000913/3312016‑3312195
                                                                                          |                                                                                         
 cGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACt                                                                                        <  1:2104310/93‑1 (MQ=255)
  gTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGATTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTg                                                                                       >  1:2725166/1‑93 (MQ=255)
   tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTgg                                                                                      >  2:7850108/1‑93 (MQ=255)
     cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGga                                                                                    <  2:5168028/93‑1 (MQ=255)
     cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGga                                                                                    <  2:4319191/93‑1 (MQ=255)
     cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGga                                                                                    <  1:1811711/93‑1 (MQ=255)
     cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGga                                                                                    <  1:909068/93‑1 (MQ=255)
      cgcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGgag                                                                                   <  2:1746472/92‑1 (MQ=255)
       gcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGgagg                                                                                  >  1:1014680/1‑92 (MQ=255)
       gcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGgagg                                                                                  >  1:4686258/1‑92 (MQ=255)
       gcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGgaga                                                                                  <  2:3724661/93‑1 (MQ=255)
       gcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTACGGCAGGATGCCGCACTGGgagg                                                                                  >  2:8161536/1‑92 (MQ=255)
         agTTTATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCCCTGGGAGAgt                                                                                <  1:2139373/93‑1 (MQ=255)
         agTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgt                                                                                >  1:7661214/1‑93 (MQ=255)
         agTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgt                                                                                <  2:2611901/93‑1 (MQ=255)
          gTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgtt                                                                               >  1:6372213/1‑93 (MQ=255)
           ttCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttc                                                                              <  2:7951974/93‑1 (MQ=255)
           ttCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgt                                                                                <  2:8561151/91‑1 (MQ=255)
               tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcgttc                                                                          >  1:3764729/1‑93 (MQ=255)
               tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcgttc                                                                          <  2:2830115/93‑1 (MQ=255)
                cGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCt                                                                         <  1:1765699/93‑1 (MQ=255)
                cGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTGCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCt                                                                         <  1:1278973/93‑1 (MQ=255)
                  ctgccGGTTCCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACACTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTa                                                                       <  1:331707/90‑1 (MQ=255)
                   tACAGGTACCCGCTGTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCCCTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTaa                                                                      <  2:5254334/93‑1 (MQ=255)
                    aCCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAc                                                                     <  1:6927833/93‑1 (MQ=255)
                      ctgTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAcgc                                                                   <  1:1475899/91‑1 (MQ=255)
                      cGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAcgc                                                                   >  1:8280679/1‑93 (MQ=255)
                      cGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAcgc                                                                   >  2:4774790/1‑93 (MQ=255)
                         tACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGAt                                                                <  2:3788049/93‑1 (MQ=255)
                         tACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGAt                                                                <  1:5850723/93‑1 (MQ=255)
                          gcccGCGTTCGCAGATACGTGTACGAACTGGCTTGCACCGCCCCACTCGTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATc                                                               <  1:265795/92‑1 (MQ=255)
                           cccGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATcc                                                              <  1:4423821/93‑1 (MQ=255)
                            ccGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCg                                                             >  2:7587129/1‑93 (MQ=255)
                            ccGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCg                                                             <  2:5356327/93‑1 (MQ=255)
                              gCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGa                                                           <  1:3872451/93‑1 (MQ=255)
                               cTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGAt                                                          <  1:8550217/93‑1 (MQ=255)
                                  tCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAg                                                       >  2:5926475/1‑93 (MQ=255)
                                  tCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAg                                                       >  2:234690/1‑93 (MQ=255)
                                  tCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAg                                                       <  1:6024011/93‑1 (MQ=255)
                                     cAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCtt                                                    >  1:3629163/1‑93 (MQ=255)
                                      aGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTc                                                   >  2:774069/1‑93 (MQ=255)
                                      aGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTc                                                   >  2:1070619/1‑93 (MQ=255)
                                       gATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCt                                                  >  2:6119121/1‑93 (MQ=255)
                                       gATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCt                                                  <  1:578508/93‑1 (MQ=255)
                                       gATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCt                                                  >  2:4231859/1‑93 (MQ=255)
                                        aTACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTg                                                 <  1:4371638/93‑1 (MQ=255)
                                        aTACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTg                                                 <  2:2417793/93‑1 (MQ=255)
                                        aTACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTg                                                 >  1:6513025/1‑93 (MQ=255)
                                         tACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTgg                                                <  1:3099610/93‑1 (MQ=255)
                                         caCGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCATGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTgg                                                >  2:5608193/2‑93 (MQ=255)
                                              gtgcGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTAt                                           <  1:4534184/90‑1 (MQ=255)
                                              gtACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTAt                                           <  2:3152730/93‑1 (MQ=255)
                                               tACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATc                                          >  1:8190299/1‑93 (MQ=255)
                                               tACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATc                                          <  2:8306144/93‑1 (MQ=255)
                                                 cGAACTGGCTTTCACCGCCCCACGCTTCCGGCAGGATGCCGCACTGGTAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGa                                        <  2:4738608/93‑1 (MQ=255)
                                                  gAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGAt                                       <  1:8727491/93‑1 (MQ=255)
                                                  gAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGAt                                       >  2:6294488/1‑93 (MQ=255)
                                                   aaCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATc                                      >  2:698313/1‑93 (MQ=255)
                                                   aaCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATc                                      >  1:2023642/1‑93 (MQ=255)
                                                    aCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCt                                     <  2:2948728/93‑1 (MQ=255)
                                                     cTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCtt                                    <  2:1414276/93‑1 (MQ=255)
                                                        gCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGtt                                 >  2:3405922/1‑93 (MQ=255)
                                                          tttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGttta                               >  1:4124700/1‑91 (MQ=255)
                                                          tttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCa                               >  1:3955419/1‑93 (MQ=255)
                                                          tttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCa                               <  2:1540600/93‑1 (MQ=255)
                                                          cttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCa                               <  1:709392/92‑1 (MQ=255)
                                                           ttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGCGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCat                              >  1:7602262/1‑92 (MQ=255)
                                                           ttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCAc                              <  1:6761909/93‑1 (MQ=255)
                                                              aCCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGc                           >  1:1412600/1‑93 (MQ=255)
                                                                cGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCaa                         >  1:3804445/1‑93 (MQ=255)
                                                                cGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCaa                         <  1:5894255/93‑1 (MQ=255)
                                                                cGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCaa                         <  1:2390152/93‑1 (MQ=255)
                                                                 gCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAac                        <  1:5420075/93‑1 (MQ=255)
                                                                  ccccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacc                       >  1:5644303/1‑93 (MQ=255)
                                                                  ccccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacc                       >  1:5056058/1‑93 (MQ=255)
                                                                  ccccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacc                       >  1:5134022/1‑93 (MQ=255)
                                                                  ccccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacc                       >  2:4113663/1‑93 (MQ=255)
                                                                  ccccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacc                       >  1:1674274/1‑93 (MQ=255)
                                                                   cccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccc                      >  2:7261664/1‑92 (MQ=255)
                                                                   cccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacca                      <  2:7640966/93‑1 (MQ=255)
                                                                    cccctctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTGAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccac                     <  1:5720532/90‑1 (MQ=255)
                                                                    ccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATTTTGTTTACTGCAaacac                     >  2:6658935/1‑93 (MQ=255)
                                                                     cACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacc                    <  2:6919283/93‑1 (MQ=255)
                                                                     cACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacc                    >  1:7193362/1‑93 (MQ=255)
                                                                     cACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacc                    <  2:7321874/93‑1 (MQ=255)
                                                                     cACTCTTCCGGCAGGATGCCGCACTGGGAGAGGTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacc                    >  1:7792587/1‑93 (MQ=255)
                                                                      aCTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacca                   <  2:5814647/93‑1 (MQ=255)
                                                                      aCTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacca                   <  2:1287623/93‑1 (MQ=255)
                                                                      aCTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacca                   >  2:6572310/1‑93 (MQ=255)
                                                                      aCTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacca                   >  1:3342564/1‑93 (MQ=255)
                                                                       ctctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccaccac                  <  2:4637302/93‑1 (MQ=255)
                                                                        tctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccaccacc                 >  1:1595921/1‑93 (MQ=255)
                                                                              ggCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTAcc           >  2:8764804/1‑93 (MQ=255)
                                                                              ggCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTAcc           >  1:110/1‑93 (MQ=255)
                                                                               gCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCt          >  1:6136513/1‑93 (MQ=255)
                                                                                 aGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgc        >  2:7109075/1‑93 (MQ=255)
                                                                                  ggATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgcg       >  2:40131/1‑93 (MQ=255)
                                                                                  ggATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgcg       >  2:2021681/1‑93 (MQ=255)
                                                                                    aTGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGcgcc     <  1:5528895/93‑1 (MQ=255)
                                                                                      gCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGcgccgc   >  2:1930014/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCT  >  NC_000913/3312016‑3312195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 201 total aligned reads displayed.
ATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCG  >  NC_000913/3312013‑3312199
                                                                                             |                                                                                             
ATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGC                                                                                               >  SRR4302541.6926010/1‑94 (MQ=60)
 TAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCA                                                                                              >  SRR4302541.6912377/1‑94 (MQ=60)
    CGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTG                                                                                           <  SRR4302541.2124705/94‑1 (MQ=60)
    CGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGATTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTG                                                                                           >  SRR4302541.2752403/1‑94 (MQ=60)
     GTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGG                                                                                          >  SRR4302541.6341959/1‑94 (MQ=60)
      TCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGG                                                                                         >  SRR4302541.7049872/1‑94 (MQ=60)
       CCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGG                                                                                        >  SRR4302541.2604816/1‑94 (MQ=60)
       CCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGA                                                                                        <  SRR4302541.1303012/94‑1 (MQ=60)
        CAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAG                                                                                       >  SRR4302541.1100148/1‑94 (MQ=60)
        CAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAG                                                                                       <  SRR4302541.917529/94‑1 (MQ=60)
        CAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAG                                                                                       <  SRR4302541.1829328/94‑1 (MQ=60)
        CAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAG                                                                                       >  SRR4302541.5725157/1‑94 (MQ=60)
         AGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTGACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGA                                                                                      <  SRR4302541.5381122/94‑1 (MQ=60)
         AGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGG                                                                                      >  SRR4302541.1024061/1‑94 (MQ=60)
         AGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGG                                                                                      >  SRR4302541.6785566/1‑94 (MQ=60)
         AGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGG                                                                                      >  SRR4302541.6849547/1‑94 (MQ=60)
         AGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGG                                                                                      >  SRR4302541.4733989/1‑94 (MQ=60)
         AGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGG                                                                                      >  SRR4302541.4292260/1‑94 (MQ=60)
          GCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCAGGCAGGATGCCGCACTGGGAGAG                                                                                     <  SRR4302541.1667150/94‑1 (MQ=60)
           CAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGT                                                                                    >  SRR4302541.7742752/1‑94 (MQ=60)
            AGTTTATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCCCTGGGAGAGTT                                                                                   <  SRR4302541.2160061/94‑1 (MQ=60)
            AGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTT                                                                                   >  SRR4302541.6437331/1‑94 (MQ=60)
             GTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTC                                                                                  >  SRR4302541.753928/1‑94 (MQ=60)
             GTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTA                                                                                  >  SRR4302541.8653129/1‑94 (MQ=60)
              TTCATCGATACCGGTACCCGCTTTCGCAGATCCGTGTACGAACTGGCTTTCCCCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCG                                                                                 <  SRR4302541.2175507/94‑1 (MQ=60)
               TCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGT                                                                                >  SRR4302541.6216011/1‑94 (MQ=60)
                CATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTT                                                                               <  SRR4302541.1884134/94‑1 (MQ=60)
                 CTCGATGCCGGTAGCCGCTTTCGCAGATACGTGTACGAACTTGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTC                                                                              <  SRR4302541.1909796/94‑1 (MQ=60)
                 ATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTC                                                                              <  SRR4302541.1821422/94‑1 (MQ=60)
                 ATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTC                                                                              >  SRR4302541.3802542/1‑94 (MQ=60)
                  TCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCT                                                                             <  SRR4302541.4629942/94‑1 (MQ=60)
                  TCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCAACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCT                                                                             <  SRR4302541.7218008/94‑1 (MQ=60)
                   CGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTT                                                                            <  SRR4302541.1782878/94‑1 (MQ=60)
                   CGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTT                                                                            <  SRR4302541.212562/94‑1 (MQ=60)
                   CGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTGCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTT                                                                            <  SRR4302541.1291665/94‑1 (MQ=60)
                     ctgCCGGTTCCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACACTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAA                                                                          <  SRR4302541.334685/91‑1 (MQ=60)
                     GTACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAA                                                                          <  SRR4302541.334687/94‑1 (MQ=60)
                     ATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAA                                                                          <  SRR4302541.3715148/94‑1 (MQ=60)
                       TCCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                        <  SRR4302541.5097538/94‑1 (MQ=60)
                       ACCGGTGCCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                        <  SRR4302541.6091345/94‑1 (MQ=60)
                       ACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTgacgg                                                                        >  SRR4302541.1822723/1‑89 (MQ=60)
                       ACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                        <  SRR4302541.2253753/94‑1 (MQ=60)
                       ACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                        <  SRR4302541.7001760/94‑1 (MQ=60)
                       ACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                        <  SRR4302541.1346033/94‑1 (MQ=60)
                        CCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGC                                                                       >  SRR4302541.8370758/1‑94 (MQ=60)
                         CTGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCG                                                                      <  SRR4302541.1490379/94‑1 (MQ=60)
                         CGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCG                                                                      <  SRR4302541.1490610/94‑1 (MQ=60)
                          GGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGA                                                                     >  SRR4302541.8025145/1‑94 (MQ=60)
                            TACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATC                                                                   <  SRR4302541.5910219/94‑1 (MQ=60)
                             TCCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCC                                                                  <  SRR4302541.7461140/94‑1 (MQ=60)
                             GCCCGCGTTCGCAGATACGTGTACGAACTGGCTTGCACCGCCCCACTCGTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCC                                                                  <  SRR4302541.267975/94‑1 (MQ=60)
                             ACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCC                                                                  <  SRR4302541.565720/94‑1 (MQ=60)
                              tcccgTTTCGCAGATACGTGTACGAACTTGCCTTCACCCCCCCCCCCTTCCGGCAGGATGCCGCCCTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                 <  SRR4302541.4468248/89‑1 (MQ=60)
                              CCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                 >  SRR4302541.5118447/1‑94 (MQ=60)
                              CCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                 >  SRR4302541.6193399/1‑94 (MQ=60)
                              CCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                 <  SRR4302541.4468247/94‑1 (MQ=60)
                              CCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                 <  SRR4302541.1830079/94‑1 (MQ=60)
                              CCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                 >  SRR4302541.5871947/1‑94 (MQ=60)
                               CCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGG                                                                <  SRR4302541.5489017/94‑1 (MQ=60)
                                CGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGA                                                               <  SRR4302541.2637819/94‑1 (MQ=60)
                                CGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGA                                                               <  SRR4302541.7800768/94‑1 (MQ=60)
                                CGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGA                                                               <  SRR4302541.3853341/94‑1 (MQ=60)
                                 GCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGAT                                                              >  SRR4302541.3939403/1‑94 (MQ=60)
                                 GCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGAT                                                              <  SRR4302541.3911384/94‑1 (MQ=60)
                                 GCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGAT                                                              <  SRR4302541.1039833/94‑1 (MQ=60)
                                 GCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGAT                                                              >  SRR4302541.2564471/1‑94 (MQ=60)
                                  CTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATC                                                             <  SRR4302541.2139973/94‑1 (MQ=60)
                                  CTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATC                                                             >  SRR4302541.2750199/1‑94 (MQ=60)
                                  CTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATC                                                             <  SRR4302541.5877019/94‑1 (MQ=60)
                                  CTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATC                                                             <  SRR4302541.8642116/94‑1 (MQ=60)
                                   TTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCA                                                            <  SRR4302541.1699038/94‑1 (MQ=60)
                                    TTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAG                                                           <  SRR4302541.3791709/94‑1 (MQ=60)
                                     TCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGC                                                          <  SRR4302541.6591200/94‑1 (MQ=60)
                                     TCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGC                                                          <  SRR4302541.6085736/94‑1 (MQ=60)
                                      CGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCT                                                         >  SRR4302541.4455813/1‑94 (MQ=60)
                                      CGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCT                                                         <  SRR4302541.1361848/94‑1 (MQ=60)
                                      CGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCT                                                         <  SRR4302541.4112472/94‑1 (MQ=60)
                                       GCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTT                                                        <  SRR4302541.6180662/94‑1 (MQ=60)
                                       GCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTT                                                        <  SRR4302541.3325080/94‑1 (MQ=60)
                                       GCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTT                                                        <  SRR4302541.4075987/94‑1 (MQ=60)
                                       GCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTT                                                        >  SRR4302541.3665951/1‑94 (MQ=60)
                                        CAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTC                                                       <  SRR4302541.5964298/94‑1 (MQ=60)
                                        CAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTC                                                       >  SRR4302541.7219917/1‑94 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCTCTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                      <  SRR4302541.1649770/94‑1 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCTCTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                      <  SRR4302541.1509986/94‑1 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                      <  SRR4302541.5520214/94‑1 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                      <  SRR4302541.4425283/94‑1 (MQ=60)
                                          GATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTG                                                     >  SRR4302541.6579484/1‑94 (MQ=60)
                                          GATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTG                                                     >  SRR4302541.6579487/1‑94 (MQ=60)
                                          GATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTG                                                     <  SRR4302541.583956/94‑1 (MQ=60)
                                          GATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTG                                                     >  SRR4302541.5837859/1‑94 (MQ=60)
                                          GATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTG                                                     <  SRR4302541.5820977/94‑1 (MQ=60)
                                          GATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTG                                                     <  SRR4302541.3971168/94‑1 (MQ=60)
                                          GATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGGTCTTAACGCGATCCGGATCAGCTTCTG                                                     >  SRR4302541.8536077/1‑94 (MQ=60)
                                           ATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGG                                                    <  SRR4302541.4415316/94‑1 (MQ=60)
                                           ATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGG                                                    <  SRR4302541.3744014/94‑1 (MQ=60)
                                           ATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGG                                                    >  SRR4302541.5205607/1‑94 (MQ=60)
                                            TACGTGTACGAACTGGCTTTCACCGCCCCCCTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGT                                                   <  SRR4302541.447791/94‑1 (MQ=60)
                                            TACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGT                                                   <  SRR4302541.3131027/94‑1 (MQ=60)
                                            TACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGT                                                   <  SRR4302541.4013952/94‑1 (MQ=60)
                                            TACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGGTCGTTCTTAACGCGATCCGGATCAGCTTCTGGT                                                   >  SRR4302541.779696/1‑94 (MQ=60)
                                             ACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTT                                                  <  SRR4302541.8384596/94‑1 (MQ=60)
                                             ACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTT                                                  <  SRR4302541.5069370/94‑1 (MQ=60)
                                              CGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTT                                                 >  SRR4302541.871183/1‑94 (MQ=60)
                                               GTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTA                                                <  SRR4302541.7706436/94‑1 (MQ=60)
                                                TGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTAT                                               >  SRR4302541.8330395/1‑94 (MQ=60)
                                                TGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTAT                                               >  SRR4302541.2882861/1‑94 (MQ=60)
                                                 GTGCGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATC                                              <  SRR4302541.4579990/94‑1 (MQ=60)
                                                 GTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATC                                              >  SRR4302541.8279699/1‑94 (MQ=60)
                                                 GTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATC                                              >  SRR4302541.3985633/1‑94 (MQ=60)
                                                 GTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATC                                              >  SRR4302541.5949200/1‑94 (MQ=60)
                                                  TACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCG                                             <  SRR4302541.3895110/94‑1 (MQ=60)
                                                    CGAACTGGCTTGCACCGCCCCACTCTTCCGGCAGGATGCCGCGCTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGAT                                           <  SRR4302541.7085302/94‑1 (MQ=60)
                                                     GAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATC                                          >  SRR4302541.2043211/1‑94 (MQ=60)
                                                     GAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATC                                          <  SRR4302541.8820877/94‑1 (MQ=60)
                                                      AACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCT                                         <  SRR4302541.8105334/94‑1 (MQ=60)
                                                      AACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCT                                         >  SRR4302541.3480838/1‑94 (MQ=60)
                                                       ACTGGTTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTGTATCGATCTT                                        <  SRR4302541.3568974/94‑1 (MQ=60)
                                                       ACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTT                                        <  SRR4302541.5951187/94‑1 (MQ=60)
                                                        CTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTG                                       >  SRR4302541.4606205/1‑94 (MQ=60)
                                                        CTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTG                                       <  SRR4302541.1564079/94‑1 (MQ=60)
                                                        CTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTG                                       >  SRR4302541.524571/1‑94 (MQ=60)
                                                         TGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGT                                      >  SRR4302541.929562/1‑94 (MQ=60)
                                                         TGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGT                                      >  SRR4302541.1158715/1‑94 (MQ=60)
                                                           GCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTC                                    <  SRR4302541.5177646/94‑1 (MQ=60)
                                                           GCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTC                                    <  SRR4302541.3250345/94‑1 (MQ=60)
                                                           GCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTC                                    <  SRR4302541.8882175/94‑1 (MQ=60)
                                                            CTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTTA                                   >  SRR4302541.4165445/1‑94 (MQ=60)
                                                            CTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCA                                   >  SRR4302541.3994731/1‑94 (MQ=60)
                                                             TTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGCGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCAT                                  >  SRR4302541.7683491/1‑94 (MQ=60)
                                                             CTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCAC                                  <  SRR4302541.716069/94‑1 (MQ=60)
                                                              TTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACT                                 <  SRR4302541.6834217/94‑1 (MQ=60)
                                                              TTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACT                                 <  SRR4302541.6040957/94‑1 (MQ=60)
                                                              TTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACT                                 <  SRR4302541.6234638/94‑1 (MQ=60)
                                                               TCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTG                                >  SRR4302541.5928246/1‑94 (MQ=60)
                                                                CACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGC                               >  SRR4302541.7705834/1‑94 (MQ=60)
                                                                CACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGC                               >  SRR4302541.1426362/1‑94 (MQ=60)
                                                                 CCCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCA                              <  SRR4302541.2061888/94‑1 (MQ=60)
                                                                 CCCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCA                              <  SRR4302541.1736503/94‑1 (MQ=60)
                                                                 ACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCA                              >  SRR4302541.3812521/1‑94 (MQ=60)
                                                                 ACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCA                              <  SRR4302541.5777027/94‑1 (MQ=60)
                                                                 ACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCA                              <  SRR4302541.8902908/94‑1 (MQ=60)
                                                                  CCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAA                             >  SRR4302541.3842741/1‑94 (MQ=60)
                                                                  CCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAA                             >  SRR4302541.3842739/1‑94 (MQ=60)
                                                                   CGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAAC                            >  SRR4302541.7967478/1‑94 (MQ=60)
                                                                   CGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAAC                            >  SRR4302541.7591505/1‑94 (MQ=60)
                                                                   CGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAAC                            <  SRR4302541.4235209/94‑1 (MQ=60)
                                                                   CGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAAC                            <  SRR4302541.2413540/94‑1 (MQ=60)
                                                                   CGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAAC                            <  SRR4302541.5954235/94‑1 (MQ=60)
                                                                    GCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACC                           >  SRR4302541.5186808/1‑94 (MQ=60)
                                                                    GCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACC                           >  SRR4302541.5701982/1‑94 (MQ=60)
                                                                    GCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACC                           <  SRR4302541.5475671/94‑1 (MQ=60)
                                                                    GCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACC                           >  SRR4302541.4466542/1‑94 (MQ=60)
                                                                    GCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACC                           >  SRR4302541.5108069/1‑94 (MQ=60)
                                                                    GCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACC                           >  SRR4302541.1690763/1‑94 (MQ=60)
                                                                     CCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTCTAGATCTTGTTCACTGCAACCA                          >  SRR4302541.1194709/1‑94 (MQ=60)
                                                                     CCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCA                          >  SRR4302541.1194711/1‑94 (MQ=60)
                                                                      CCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCAC                         >  SRR4302541.2697007/1‑94 (MQ=60)
                                                                       CCCCTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTGAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                        <  SRR4302541.5778844/94‑1 (MQ=60)
                                                                       CCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                        <  SRR4302541.830028/94‑1 (MQ=60)
                                                                       CCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                        >  SRR4302541.7270090/1‑94 (MQ=60)
                                                                       CCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                        >  SRR4302541.3499696/1‑94 (MQ=60)
                                                                       CCACTCTTCCGGCAGGATGCCGCACTGGGAGAGGTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                        >  SRR4302541.7876114/1‑94 (MQ=60)
                                                                        CGCTCTTCCGGCAGGAGGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCA                       <  SRR4302541.1819206/94‑1 (MQ=60)
                                                                        CACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCA                       >  SRR4302541.4505193/1‑94 (MQ=60)
                                                                        CACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCA                       >  SRR4302541.3375993/1‑94 (MQ=60)
                                                                        CACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCA                       <  SRR4302541.3331111/94‑1 (MQ=60)
                                                                        CACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCA                       <  SRR4302541.6556025/94‑1 (MQ=60)
                                                                        CACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCA                       >  SRR4302541.4841334/1‑94 (MQ=60)
                                                                         ACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCCCCAC                      >  SRR4302541.6869846/1‑94 (MQ=60)
                                                                         ACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCAC                      >  SRR4302541.3358373/1‑94 (MQ=60)
                                                                         ACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCAC                      >  SRR4302541.6546423/1‑94 (MQ=60)
                                                                         ACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGCTTATCGATCTTGTTCACTGCAACCACCAC                      >  SRR4302541.1804809/1‑94 (MQ=60)
                                                                          CTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACC                     <  SRR4302541.7000940/94‑1 (MQ=60)
                                                                          CTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACC                     >  SRR4302541.1611812/1‑94 (MQ=60)
                                                                            CTTCCGGCAGGATGCCGCGCTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGG                   <  SRR4302541.5157323/94‑1 (MQ=60)
                                                                            CTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGG                   >  SRR4302541.5846891/1‑94 (MQ=60)
                                                                             TTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGT                  <  SRR4302541.4472447/94‑1 (MQ=60)
                                                                             TTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGT                  <  SRR4302541.2086669/94‑1 (MQ=60)
                                                                             TTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGT                  <  SRR4302541.2974312/94‑1 (MQ=60)
                                                                                CGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACC               <  SRR4302541.7138808/94‑1 (MQ=60)
                                                                                CGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACC               >  SRR4302541.114/1‑94 (MQ=60)
                                                                                 GGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCT              >  SRR4302541.6199175/1‑94 (MQ=60)
                                                                                   CAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGC            >  SRR4302541.5687830/1‑94 (MQ=60)
                                                                                   CAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGC            >  SRR4302541.896501/1‑94 (MQ=60)
                                                                                   CAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGC            >  SRR4302541.3820450/1‑94 (MQ=60)
                                                                                   CAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCAACACCGGTACCTGC            >  SRR4302541.8596040/1‑94 (MQ=60)
                                                                                    AGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG           >  SRR4302541.1488938/1‑94 (MQ=60)
                                                                                    AGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG           <  SRR4302541.5768128/94‑1 (MQ=60)
                                                                                    AGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG           >  SRR4302541.3107828/1‑94 (MQ=60)
                                                                                    AGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG           >  SRR4302541.7722697/1‑94 (MQ=60)
                                                                                    AGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTATGGTTGATAGAACTTGTTCACTGCAACCACCACCGGCACCTGCG           >  SRR4302541.1488940/1‑94 (MQ=60)
                                                                                     GGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGC          <  SRR4302541.5636556/94‑1 (MQ=60)
                                                                                      GATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCC         <  SRR4302541.2351861/94‑1 (MQ=60)
                                                                                      GATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCC         >  SRR4302541.1661382/1‑94 (MQ=60)
                                                                                       ATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCG        <  SRR4302541.5585495/94‑1 (MQ=60)
                                                                                        TGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGC       >  SRR4302541.2094947/1‑94 (MQ=60)
                                                                                          CCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTT     <  SRR4302541.8306796/94‑1 (MQ=60)
                                                                                           CGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTT    >  SRR4302541.562423/1‑94 (MQ=60)
                                                                                             CACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCG  <  SRR4302541.6473567/94‑1 (MQ=60)
                                                                                             |                                                                                             
ATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTCG  >  NC_000913/3312013‑3312199

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑