Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,312,106 T→C Y517C (TAC→TGC)  infB ← protein chain initiation factor IF‑2

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,312,1060TC100.0% 972.0 / NA 278Y517C (TAC→TGC) infBprotein chain initiation factor IF‑2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (111/167);  total (111/167)

Only 100 of 282 total aligned reads displayed.

GCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCT  >  NC_000913/3312016‑3312195
                                                                                          |                                                                                         
   tCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGTCGCACTgg                                                                                      >  1:3469508/1‑93 (MQ=255)
    ccAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTggg                                                                                     <  2:5396060/93‑1 (MQ=255)
     cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGga                                                                                    <  1:2843616/93‑1 (MQ=255)
     cagcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTCCCGGCAGGATGCCGCACTGGga                                                                                    <  2:4967445/93‑1 (MQ=255)
      agcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGgag                                                                                   >  1:4829231/1‑93 (MQ=255)
      agcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGgag                                                                                   >  2:5455831/1‑93 (MQ=255)
       gcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGgaga                                                                                  <  2:2588111/93‑1 (MQ=255)
       gcagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGgaga                                                                                  <  2:2818456/93‑1 (MQ=255)
        cagTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGagag                                                                                 <  1:1407752/93‑1 (MQ=255)
         agTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgt                                                                                <  2:2338115/93‑1 (MQ=255)
           ttCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttc                                                                              <  1:1754245/93‑1 (MQ=255)
           ttCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAggtc                                                                              >  1:3541671/1‑93 (MQ=255)
            tCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcg                                                                             <  1:4476249/93‑1 (MQ=255)
            tCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcg                                                                             <  1:558458/93‑1 (MQ=255)
             cATCGATACCGGTCCCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcgt                                                                            <  2:5954313/93‑1 (MQ=255)
             cATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcgt                                                                            <  2:2694711/93‑1 (MQ=255)
               tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcgttc                                                                          <  1:4037346/93‑1 (MQ=255)
               tCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAgttcgttc                                                                          <  2:4370561/93‑1 (MQ=255)
                cGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCt                                                                         <  2:5689863/93‑1 (MQ=255)
                   tACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTaa                                                                      <  1:5489152/93‑1 (MQ=255)
                    aCCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAc                                                                     <  2:3074155/93‑1 (MQ=255)
                    aCCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAc                                                                     <  1:1584297/93‑1 (MQ=255)
                       ggTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAcgcg                                                                  >  1:3947278/1‑93 (MQ=255)
                        gTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGa                                                                 >  2:1534384/1‑93 (MQ=255)
                         tACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGAt                                                                <  2:63778/93‑1 (MQ=255)
                         tACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGAt                                                                <  1:1552066/93‑1 (MQ=255)
                          aCCCGCTTTCGCAGATACGTGTGCGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATc                                                               <  2:2085207/93‑1 (MQ=255)
                          aCCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATc                                                               <  1:5118402/93‑1 (MQ=255)
                          aCCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATc                                                               <  2:2399862/93‑1 (MQ=255)
                           cccGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATcc                                                              <  1:64356/93‑1 (MQ=255)
                            ccGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCg                                                             >  1:1020095/1‑93 (MQ=255)
                             cGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCgg                                                            <  2:123851/93‑1 (MQ=255)
                              gCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGa                                                           <  1:2396115/93‑1 (MQ=255)
                               cTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGAt                                                          <  1:4114643/93‑1 (MQ=255)
                                tttCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATc                                                         >  2:5535327/1‑93 (MQ=255)
                                tttCGCAGATACGTGTACGAACCGGCTTCCACAGCCCCACTCTTCCGGCAGGATGCCGCATTGGGAGAGTTCGTTCTTAACGCGATCCGGATc                                                         <  2:3164727/93‑1 (MQ=255)
                                  tCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAg                                                       >  1:621653/1‑93 (MQ=255)
                                   cGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGc                                                      >  1:700488/1‑93 (MQ=255)
                                    gCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCt                                                     <  2:5428722/93‑1 (MQ=255)
                                     cAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCtt                                                    >  1:1955408/1‑93 (MQ=255)
                                      aGATACGTGTGCGAACTGACTTTCACCGCCCCCCCCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTc                                                   <  1:2945843/93‑1 (MQ=255)
                                       gATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCt                                                  <  2:901841/93‑1 (MQ=255)
                                       gATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCt                                                  <  1:2656778/93‑1 (MQ=255)
                                        aTACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTg                                                 <  1:4268598/93‑1 (MQ=255)
                                           cGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGtt                                              <  2:4105397/93‑1 (MQ=255)
                                             tgtACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTa                                            <  2:1748303/93‑1 (MQ=255)
                                             tgtACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTa                                            <  2:4710195/93‑1 (MQ=255)
                                               tACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATc                                          <  1:3065435/93‑1 (MQ=255)
                                                aCGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCg                                         <  2:4445084/93‑1 (MQ=255)
                                                 cGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGa                                        <  1:5667473/93‑1 (MQ=255)
                                                  gAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGAt                                       <  1:5384563/93‑1 (MQ=255)
                                                  gAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGAt                                       <  2:5129778/93‑1 (MQ=255)
                                                   aaCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATc                                      >  2:4179288/1‑93 (MQ=255)
                                                   aaCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATc                                      <  1:5728033/93‑1 (MQ=255)
                                                    aCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCt                                     <  1:5197595/93‑1 (MQ=255)
                                                    aCTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCt                                     <  1:2354659/93‑1 (MQ=255)
                                                     cTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCtt                                    <  1:4663192/93‑1 (MQ=255)
                                                     cTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCtt                                    <  2:6169339/93‑1 (MQ=255)
                                                      tGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTg                                   <  2:3536320/93‑1 (MQ=255)
                                                      tGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTg                                   >  1:1088431/1‑93 (MQ=255)
                                                        gCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGtt                                 <  1:5370853/93‑1 (MQ=255)
                                                         cTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTc                                >  1:4887981/1‑93 (MQ=255)
                                                          tttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCa                               <  1:4172658/93‑1 (MQ=255)
                                                          tttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCa                               <  2:1059477/93‑1 (MQ=255)
                                                           ttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCAc                              <  2:1681841/93‑1 (MQ=255)
                                                           ttCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCAc                              <  2:4634265/93‑1 (MQ=255)
                                                            tCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACt                             <  2:177049/93‑1 (MQ=255)
                                                            tCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACt                             >  1:314112/1‑93 (MQ=255)
                                                             cACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTg                            >  2:5322966/1‑93 (MQ=255)
                                                                cGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGACCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCaa                         <  2:1376668/93‑1 (MQ=255)
                                                                 gCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAac                        >  1:2515747/1‑93 (MQ=255)
                                                                  ccccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacc                       <  1:5617514/93‑1 (MQ=255)
                                                                  ccccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATACGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacc                       >  1:2244551/1‑93 (MQ=255)
                                                                   cccACTCTTCCGGCAGGATGTCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAacca                      >  1:215121/1‑93 (MQ=255)
                                                                    ccACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccac                     <  1:5271338/93‑1 (MQ=255)
                                                                     cACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacc                    <  2:1318474/93‑1 (MQ=255)
                                                                     cACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacc                    >  1:3777957/1‑93 (MQ=255)
                                                                     cACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccacc                    >  1:1211456/1‑93 (MQ=255)
                                                                        tctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccaccacc                 >  2:3308012/1‑93 (MQ=255)
                                                                        tctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccaccacc                 >  2:732967/1‑93 (MQ=255)
                                                                        tctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAaccaccacc                 >  1:4010567/1‑93 (MQ=255)
                                                                         ctTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCg                >  2:2019150/1‑93 (MQ=255)
                                                                          ttCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCgg               <  2:6010820/93‑1 (MQ=255)
                                                                          ttCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCgg               <  1:2223672/93‑1 (MQ=255)
                                                                          ttCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCgg               >  2:4838483/1‑93 (MQ=255)
                                                                          ttCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCgg               <  1:4444618/93‑1 (MQ=255)
                                                                              ggCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTAcc           >  2:771737/1‑93 (MQ=255)
                                                                               gCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCt          >  2:1460241/1‑93 (MQ=255)
                                                                               gCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCt          <  2:1499321/93‑1 (MQ=255)
                                                                                 aGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgc        >  1:2045496/1‑93 (MQ=255)
                                                                                 aGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgc        <  1:1030991/93‑1 (MQ=255)
                                                                                 aGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgc        <  2:559638/93‑1 (MQ=255)
                                                                                  ggATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgcg       >  2:2456743/1‑93 (MQ=255)
                                                                                   gATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTgcgc      <  1:5886929/93‑1 (MQ=255)
                                                                                    aTGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGcgcc     <  2:4571518/93‑1 (MQ=255)
                                                                                     tGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGcgccg    >  1:4078958/1‑93 (MQ=255)
                                                                                      gCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGcgccgc   <  2:3828595/93‑1 (MQ=255)
                                                                                      gCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGcgccgc   >  1:2716119/1‑93 (MQ=255)
                                                                                       ccGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCt  <  1:924669/93‑1 (MQ=255)
                                                                                       ccGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCt  >  1:3364120/1‑93 (MQ=255)
                                                                                          |                                                                                         
GCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCT  >  NC_000913/3312016‑3312195

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTC  >  NC_000913/3312013‑3312198
                                                                                             |                                                                                            
ATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGC                                                                                              >  SRR4302534.4288759/1‑94 (MQ=60)
 TAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCA                                                                                             <  SRR4302534.1659994/94‑1 (MQ=60)
  AGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCAC                                                                                            <  SRR4302534.5850339/94‑1 (MQ=60)
   GCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACT                                                                                           <  SRR4302534.2945022/94‑1 (MQ=60)
     GTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGTCGCACTGG                                                                                         >  SRR4302534.3562761/1‑94 (MQ=60)
        CAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAG                                                                                      >  SRR4302534.4960265/1‑94 (MQ=60)
        CAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAG                                                                                      <  SRR4302534.2918326/94‑1 (MQ=60)
        CAGCAGTTCATCGATACCGGTACCCGATTTCGCAGATACGTGTACGAACTTGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAG                                                                                      >  SRR4302534.980691/1‑94 (MQ=60)
         AGCAGTTCATCGATACCGGGACCCGCTTTCGCAGATACGTGGACTAACTGGCTTTCACCGCCCGACTCTTCCGGCAGGATGCGGCACTGGGGGA                                                                                     >  SRR4302534.668665/1‑94 (MQ=60)
           CAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGT                                                                                   <  SRR4302534.1444777/94‑1 (MQ=60)
             GTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTC                                                                                 <  SRR4302534.4937405/94‑1 (MQ=60)
             GTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGGTC                                                                                 >  SRR4302534.3636948/1‑94 (MQ=60)
              TTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCG                                                                                <  SRR4302534.1799795/94‑1 (MQ=60)
               TCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGT                                                                               <  SRR4302534.572224/94‑1 (MQ=60)
               TCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGT                                                                               <  SRR4302534.4597337/94‑1 (MQ=60)
                CATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTT                                                                              <  SRR4302534.5425741/94‑1 (MQ=60)
                CATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTT                                                                              <  SRR4302534.3182485/94‑1 (MQ=60)
                  TCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCT                                                                            <  SRR4302534.4145589/94‑1 (MQ=60)
                   CGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTT                                                                           <  SRR4302534.4152396/94‑1 (MQ=60)
                   CGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTT                                                                           >  SRR4302534.2224396/1‑94 (MQ=60)
                   CGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTT                                                                           <  SRR4302534.5736221/94‑1 (MQ=60)
                      TACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAC                                                                        <  SRR4302534.5637190/94‑1 (MQ=60)
                      TACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAAC                                                                        <  SRR4302534.2296597/94‑1 (MQ=60)
                       ACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                       <  SRR4302534.2075172/94‑1 (MQ=60)
                       ACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                       <  SRR4302534.1625740/94‑1 (MQ=60)
                       ACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                       <  SRR4302534.1070672/94‑1 (MQ=60)
                       ACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                       <  SRR4302534.1416573/94‑1 (MQ=60)
                       ACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACG                                                                       >  SRR4302534.256927/1‑94 (MQ=60)
                         CGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCG                                                                     >  SRR4302534.4246872/1‑94 (MQ=60)
                         CGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCG                                                                     >  SRR4302534.4083003/1‑94 (MQ=60)
                         CGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCG                                                                     <  SRR4302534.2743858/94‑1 (MQ=60)
                         CGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCG                                                                     >  SRR4302534.4052959/1‑94 (MQ=60)
                          GGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGA                                                                    <  SRR4302534.5207107/94‑1 (MQ=60)
                           GTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGAT                                                                   >  SRR4302534.1845212/1‑94 (MQ=60)
                           GTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGAT                                                                   <  SRR4302534.3315983/94‑1 (MQ=60)
                            TACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATC                                                                  <  SRR4302534.579229/94‑1 (MQ=60)
                            TACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATC                                                                  <  SRR4302534.1592724/94‑1 (MQ=60)
                             ACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCC                                                                 <  SRR4302534.5256582/94‑1 (MQ=60)
                             ACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCC                                                                 <  SRR4302534.4541706/94‑1 (MQ=60)
                             ACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCC                                                                 <  SRR4302534.807853/94‑1 (MQ=60)
                              CCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                >  SRR4302534.1046355/1‑94 (MQ=60)
                              CCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                <  SRR4302534.66116/94‑1 (MQ=60)
                              CCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCG                                                                <  SRR4302534.5621107/94‑1 (MQ=60)
                                CGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGA                                                              <  SRR4302534.3669180/94‑1 (MQ=60)
                                CGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGA                                                              >  SRR4302534.1902820/1‑94 (MQ=60)
                                 GCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGAT                                                             <  SRR4302534.2459776/94‑1 (MQ=60)
                                  CTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATC                                                            >  SRR4302534.5592922/1‑94 (MQ=60)
                                  CTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATC                                                            <  SRR4302534.4225344/94‑1 (MQ=60)
                                  CTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATC                                                            <  SRR4302534.6205609/94‑1 (MQ=60)
                                  ATTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATC                                                            <  SRR4302534.1510119/94‑1 (MQ=60)
                                    TTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAG                                                          <  SRR4302534.4234385/94‑1 (MQ=60)
                                    TTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAG                                                          >  SRR4302534.636750/1‑94 (MQ=60)
                                     TCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGC                                                         >  SRR4302534.717651/1‑94 (MQ=60)
                                      CGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCTGCAGGATGCCGCACTGGGAGAGTTCGGTCTTAACTCGATCCGGATCAGGT                                                        >  SRR4302534.171837/1‑94 (MQ=60)
                                      CGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGGAGGATGCCGCACTGGGAGAGTTCGCTTTTAACGCGATCCCGATAAGCT                                                        >  SRR4302534.5323852/1‑94 (MQ=60)
                                       GCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTT                                                       <  SRR4302534.4544528/94‑1 (MQ=60)
                                       GCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTT                                                       >  SRR4302534.2006587/1‑94 (MQ=60)
                                         AGATACGTGTGCGAACTGACTTTCACCGCCCCCCCCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                     <  SRR4302534.3023145/94‑1 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                     >  SRR4302534.5853462/1‑94 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                     >  SRR4302534.5266586/1‑94 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                     <  SRR4302534.3551804/94‑1 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                     >  SRR4302534.5386949/1‑94 (MQ=60)
                                         AGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCT                                                     >  SRR4302534.3572528/1‑94 (MQ=60)
                                          GATACGTGTACGAACTGTCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTG                                                    <  SRR4302534.2449945/94‑1 (MQ=60)
                                          GATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTG                                                    <  SRR4302534.2727017/94‑1 (MQ=60)
                                           ATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGG                                                   >  SRR4302534.3014127/1‑94 (MQ=60)
                                           ATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGG                                                   <  SRR4302534.4383954/94‑1 (MQ=60)
                                           ATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGG                                                   <  SRR4302534.2508930/94‑1 (MQ=60)
                                           ATACGTGAACGAACTAGCTATCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGG                                                   <  SRR4302534.4992338/94‑1 (MQ=60)
                                            TACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGT                                                  <  SRR4302534.1047757/94‑1 (MQ=60)
                                            TACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGT                                                  <  SRR4302534.2607316/94‑1 (MQ=60)
                                             ACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTT                                                 <  SRR4302534.586555/94‑1 (MQ=60)
                                              CGTGTACGAACTGGCTTTCCCCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTT                                                <  SRR4302534.5816219/94‑1 (MQ=60)
                                               GTGTACGAACTGGCTTTCCCCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTA                                               <  SRR4302534.4622664/94‑1 (MQ=60)
                                               GTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTA                                               >  SRR4302534.418686/1‑94 (MQ=60)
                                                 GTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATC                                             <  SRR4302534.5647883/94‑1 (MQ=60)
                                                  TACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCG                                            <  SRR4302534.1309313/94‑1 (MQ=60)
                                                  TACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCG                                            <  SRR4302534.3145905/94‑1 (MQ=60)
                                                    CGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGAT                                          <  SRR4302534.5820906/94‑1 (MQ=60)
                                                    CGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGAT                                          <  SRR4302534.3029440/94‑1 (MQ=60)
                                                    CGAACTCGCCTTCACCGCCCCACTCTGCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAGCGCGAGACGGATCACGTTCTAGTTTATCGAT                                          <  SRR4302534.942885/94‑1 (MQ=42)
                                                     GAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATC                                         <  SRR4302534.5529596/94‑1 (MQ=60)
                                                     GAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATC                                         <  SRR4302534.5529597/94‑1 (MQ=60)
                                                     GAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATC                                         >  SRR4302534.1625109/1‑94 (MQ=60)
                                                      AACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCT                                        >  SRR4302534.5076011/1‑94 (MQ=60)
                                                      AACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCT                                        <  SRR4302534.5883240/94‑1 (MQ=60)
                                                      AACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCT                                        <  SRR4302534.3650978/94‑1 (MQ=60)
                                                      AACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCT                                        <  SRR4302534.3963362/94‑1 (MQ=60)
                                                       ACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTT                                       <  SRR4302534.5337770/94‑1 (MQ=60)
                                                       ACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTT                                       <  SRR4302534.2174127/94‑1 (MQ=60)
                                                       ACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTT                                       <  SRR4302534.2417145/94‑1 (MQ=60)
                                                       ACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTT                                       <  SRR4302534.5515658/94‑1 (MQ=60)
                                                       ACGGGCTTTCACTTCCCCACTCTTCGGGCAGGATGCCGCACTGGGAGAGTTCGTCCTTAACGCGATCCGGATGAGCTTCTGGTTTATCGATCTT                                       <  SRR4302534.5487054/94‑1 (MQ=60)
                                                        CTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTG                                      >  SRR4302534.1116740/1‑94 (MQ=60)
                                                        CTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTG                                      <  SRR4302534.2341707/94‑1 (MQ=60)
                                                        CTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTG                                      <  SRR4302534.4789800/94‑1 (MQ=60)
                                                         TGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGT                                     >  SRR4302534.4128793/1‑94 (MQ=60)
                                                          GGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTT                                    >  SRR4302534.2045783/1‑94 (MQ=60)
                                                           GCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTC                                   <  SRR4302534.4062940/94‑1 (MQ=60)
                                                           GCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTC                                   <  SRR4302534.5515583/94‑1 (MQ=60)
                                                           GCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTC                                   >  SRR4302534.5020525/1‑94 (MQ=60)
                                                             TTTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCAC                                 <  SRR4302534.4285367/94‑1 (MQ=60)
                                                              TTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATtggttttgttttct                                >  SRR4302534.6077888/1‑80 (MQ=60)
                                                              TTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACT                                >  SRR4302534.322208/1‑94 (MQ=60)
                                                              TTCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACT                                <  SRR4302534.6564258/94‑1 (MQ=60)
                                                               TCACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTG                               >  SRR4302534.1108716/1‑94 (MQ=60)
                                                                CACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGC                              >  SRR4302534.813649/1‑94 (MQ=60)
                                                                 ACCGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCA                             >  SRR4302534.286449/1‑94 (MQ=60)
                                                                   CGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAAC                           <  SRR4302534.3420582/94‑1 (MQ=60)
                                                                   CGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAAC                           >  SRR4302534.2446491/1‑94 (MQ=60)
                                                                   CGCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAAC                           >  SRR4302534.2582552/1‑94 (MQ=60)
                                                                   CACCCCACTCTTCCGGCAGGCTGCCGCCCTGGGAGAGTTCGTTCTTAACGCGATCCGGGTCAGCTTCTGGTTTATTGATCTTGATCACTGCAAC                           >  SRR4302534.1118479/1‑94 (MQ=60)
                                                                    GCCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATACGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACC                          >  SRR4302534.2304036/1‑94 (MQ=60)
                                                                     CCCCACTCTTCCGGCAGGATGTCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCA                         >  SRR4302534.220761/1‑94 (MQ=60)
                                                                     CCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCA                         >  SRR4302534.1148310/1‑94 (MQ=60)
                                                                     CCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCA                         <  SRR4302534.5769516/94‑1 (MQ=60)
                                                                     CCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCA                         >  SRR4302534.6018936/1‑94 (MQ=60)
                                                                     CCCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCA                         >  SRR4302534.4691370/1‑94 (MQ=60)
                                                                      CCCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCAC                        >  SRR4302534.4325015/1‑94 (MQ=60)
                                                                       CCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                       >  SRR4302534.224651/1‑94 (MQ=60)
                                                                       CCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                       <  SRR4302534.5413722/94‑1 (MQ=60)
                                                                       CCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                       >  SRR4302534.1243336/1‑94 (MQ=60)
                                                                       CCACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACC                       >  SRR4302534.3879379/1‑94 (MQ=60)
                                                                        CACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCCACCACCA                      >  SRR4302534.4168465/1‑94 (MQ=60)
                                                                         ACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAATCACCAC                     >  SRR4302534.5726634/1‑94 (MQ=60)
                                                                         ACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCAC                     <  SRR4302534.1175959/94‑1 (MQ=60)
                                                                         ACTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCAC                     <  SRR4302534.433228/94‑1 (MQ=60)
                                                                          CTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACC                    >  SRR4302534.4117965/1‑94 (MQ=60)
                                                                          CTCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACC                    >  SRR4302534.281885/1‑94 (MQ=60)
                                                                           TCTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCG                   <  SRR4302534.6539146/94‑1 (MQ=60)
                                                                            CTTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGG                  <  SRR4302534.2681627/94‑1 (MQ=60)
                                                                             TTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGT                 <  SRR4302534.2282560/94‑1 (MQ=60)
                                                                             TTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGT                 <  SRR4302534.4564704/94‑1 (MQ=60)
                                                                             TTCCGGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGT                 <  SRR4302534.5007882/94‑1 (MQ=60)
                                                                                 GGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCT             >  SRR4302534.3168887/1‑94 (MQ=60)
                                                                                 GGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCT             >  SRR4302534.59283/1‑94 (MQ=60)
                                                                                 GGCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCT             >  SRR4302534.4595212/1‑94 (MQ=60)
                                                                                  GCAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTG            <  SRR4302534.5354479/94‑1 (MQ=60)
                                                                                   CAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGC           >  SRR4302534.2098962/1‑94 (MQ=60)
                                                                                   CAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGC           <  SRR4302534.3594860/94‑1 (MQ=60)
                                                                                   CAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGC           >  SRR4302534.2410955/1‑94 (MQ=60)
                                                                                   CAGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGC           <  SRR4302534.4891016/94‑1 (MQ=60)
                                                                                    AGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG          <  SRR4302534.1057555/94‑1 (MQ=60)
                                                                                    AGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG          >  SRR4302534.1668605/1‑94 (MQ=60)
                                                                                    AGGATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCG          >  SRR4302534.3024242/1‑94 (MQ=60)
                                                                                      GATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCC        <  SRR4302534.6046773/94‑1 (MQ=60)
                                                                                       ATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCG       >  SRR4302534.158057/1‑94 (MQ=60)
                                                                                       ATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCG       >  SRR4302534.4188436/1‑94 (MQ=60)
                                                                                       ATGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCG       >  SRR4302534.3924148/1‑94 (MQ=60)
                                                                                        TGCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGC      >  SRR4302534.2787809/1‑94 (MQ=60)
                                                                                         GCCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCT     <  SRR4302534.2119620/94‑1 (MQ=60)
                                                                                          CCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTT    <  SRR4302534.4190753/94‑1 (MQ=60)
                                                                                          CCGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTT    <  SRR4302534.948217/94‑1 (MQ=60)
                                                                                           CGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTT   <  SRR4302534.2893871/94‑1 (MQ=60)
                                                                                           CGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTT   >  SRR4302534.5455840/1‑94 (MQ=60)
                                                                                           CGCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTT   >  SRR4302534.2053624/1‑94 (MQ=60)
                                                                                            GCACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCCCCCCCGGTACCTGCGCCGCTTTC  <  SRR4302534.3727458/94‑1 (MQ=60)
                                                                                             |                                                                                            
ATAGCGTCCAGCAGTTCATCGATACCGGTACCCGCTTTCGCAGATACGTGTACGAACTGGCTTTCACCGCCCCACTCTTCCGGCAGGATGCCGTACTGGGAGAGTTCGTTCTTAACGCGATCCGGATCAGCTTCTGGTTTATCGATCTTGTTCACTGCAACCACCACCGGTACCTGCGCCGCTTTC  >  NC_000913/3312013‑3312198

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑