Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,418,840	+G	coding (1070/1089 nt)	b3269 →	putative transport system permease protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,418,837	1	.	G	98.9%	887.1 / 0.5	260	coding (1067/1089 nt)	b3269	putative transport system permease protein
Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (127/130); total (128/132)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 296 total aligned reads displayed.

GTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑G‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC  >  NC_000913/3418745‑3418924

  aTCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑g‑g                                                                                        >  1:5995767/1‑93 (MQ=255)

  aTCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑g‑g                                                                                        >  1:6537342/1‑93 (MQ=255)

    ctcAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑g‑ggg                                                                                      <  1:3260843/93‑2 (MQ=255)

       aaCATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑g‑gggcaa                                                                                   >  2:7274325/1‑89 (MQ=255)

       aaCATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑g‑gggcaa                                                                                   <  1:4808017/93‑5 (MQ=255)

         cATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGc                                                                                 >  1:169771/1‑93 (MQ=255)

         cATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGc                                                                                 >  2:4717892/1‑93 (MQ=255)

          aTCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGTTTTCGCTGTTTGCCGGCACCGTGCTTAATCAGACGG‑GG‑CAAGcc                                                                                <  1:5406798/93‑1 (MQ=255)

          aTCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGcc                                                                                <  1:1262535/93‑1 (MQ=255)

           tCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCa                                                                               >  2:8701072/1‑93 (MQ=255)

            cGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCAt                                                                              <  2:1510618/93‑1 (MQ=255)

              tCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCg                                                                            <  1:485613/93‑1 (MQ=255)

               cAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCga                                                                           <  1:3175025/93‑1 (MQ=255)

               cAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCga                                                                           >  2:7073814/1‑93 (MQ=255)

                 aaaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCCCCGTGCTGAATCAGACGG‑GG‑CCAGCCATCgcgc                                                                         >  2:1423414/1‑90 (MQ=255)

                  aaaaTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGAc                                                                        >  2:5984422/1‑93 (MQ=255)

                  aaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGAc                                                                        <  1:8256756/93‑1 (MQ=255)

                  aaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGAc                                                                        <  2:6550893/93‑1 (MQ=255)

                  aaaCTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGAc                                                                        >  2:1942784/1‑93 (MQ=255)

                     ctcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGAt                                                                     <  2:8301868/93‑1 (MQ=255)

                      tcctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATa                                                                    >  1:5342855/1‑93 (MQ=255)

                      ccctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTTTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATa                                                                    <  2:5676431/92‑1 (MQ=255)

                      ccctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATa                                                                    <  2:2105169/92‑1 (MQ=255)

                      ccctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATa                                                                    <  1:2759049/92‑1 (MQ=255)

                       cctcTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAg                                                                   <  2:5318749/93‑1 (MQ=255)

                        ctctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGc                                                                  <  2:4916742/93‑1 (MQ=255)

                          ctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCa                                                                <  1:1138225/93‑1 (MQ=255)

                          ctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCa                                                                <  1:7688605/93‑1 (MQ=255)

                          ctctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCa                                                                <  1:6944423/93‑1 (MQ=255)

                            ctTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATg                                                              <  2:2606175/93‑1 (MQ=255)

                            cgtGCCGCCGCGATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATg                                                              <  1:8723076/91‑1 (MQ=255)

                                 cgccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCAt                                                         <  2:1547597/93‑1 (MQ=255)

                                   gcgcAATTGGCTATCCCGATATTGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGt                                                       <  2:644874/92‑1 (MQ=255)

                                   ccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGt                                                       <  2:2466047/93‑1 (MQ=255)

                                   ccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGt                                                       <  1:3827086/93‑1 (MQ=255)

                                   ccgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACCATAGCCATGACCATGt                                                       <  2:5791349/93‑1 (MQ=255)

                                    cgcTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTc                                                      <  2:80837/93‑1 (MQ=255)

                                       tATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGt                                                   >  1:628625/1‑93 (MQ=255)

                                       tATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGt                                                   >  2:1361730/1‑93 (MQ=255)

                                        tttGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTc                                                  <  1:1863477/92‑1 (MQ=255)

                                        aTTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTc                                                  >  2:4310505/1‑93 (MQ=255)

                                        aTTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTc                                                  >  2:6932232/1‑93 (MQ=255)

                                        aTTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTc                                                  <  1:4493244/93‑1 (MQ=255)

                                         ttGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCt                                                 >  2:5739885/1‑93 (MQ=255)

                                           ggCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTAt                                               >  2:6150729/1‑93 (MQ=255)

                                            gCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATc                                              <  1:7086111/93‑1 (MQ=255)

                                            gCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATc                                              <  2:2803578/93‑1 (MQ=255)

                                            gCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATc                                              <  1:5088456/93‑1 (MQ=255)

                                             cTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCt                                             <  1:1955145/93‑1 (MQ=255)

                                             cTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCt                                             <  2:377601/93‑1 (MQ=255)

                                             cTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCt                                             <  1:8031039/93‑1 (MQ=255)

                                             cTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCt                                             >  1:6664358/1‑93 (MQ=255)

                                             cTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCt                                             >  1:7481946/1‑93 (MQ=255)

                                             cTATCCCGATATGGTTTCGCTGTTGGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCt                                             <  1:3539917/93‑1 (MQ=255)

                                             cTATCCCGATATGGGTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCCGGACGATAGCCATGAACATGTCGGTCTATCt                                             >  2:4425378/1‑93 (MQ=255)

                                               aTCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGa                                           >  2:8032301/1‑93 (MQ=255)

                                               aTCCCGATATGGTTTCGCTGTGTGCCGGCACCGTGCTGAATCAGCCGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGa                                           <  2:5040938/93‑1 (MQ=255)

                                                tCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAt                                          >  2:862406/1‑93 (MQ=255)

                                                tCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAt                                          >  2:2071550/1‑93 (MQ=255)

                                                 cccGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGAtt                                         >  1:2545961/1‑93 (MQ=255)

                                                  ccGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTa                                        >  2:3006788/1‑93 (MQ=255)

                                                    gATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATc                                      >  2:2238138/1‑93 (MQ=255)

                                                    gATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATc                                      >  1:5213752/1‑93 (MQ=255)

                                                     atatGGTTTCGCTGTTTGACGGCACCGTGCTGCATCAGACGG‑GG‑CAAGCCATTGAGACGAGAGCCATGACTATGGCGGTCTATCTAATTAGCa                                     >  1:4949326/1‑93 (MQ=255)

                                                      tatGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAg                                    >  1:2263947/1‑93 (MQ=255)

                                                      tatGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAg                                    >  2:5505307/1‑93 (MQ=255)

                                                       atGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGc                                   <  2:1754295/93‑1 (MQ=255)

                                                       atGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGc                                   >  1:8612334/1‑93 (MQ=255)

                                                       atGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGc                                   <  1:8181400/93‑1 (MQ=255)

                                                         ggTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCt                                 >  2:3575386/1‑93 (MQ=255)

                                                          gTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTg                                >  2:7425213/1‑93 (MQ=255)

                                                            ttCGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGAc                              <  1:7364142/93‑1 (MQ=255)

                                                              cGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTa                            <  2:7530046/93‑1 (MQ=255)

                                                              cGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTa                            <  2:8814964/93‑1 (MQ=255)

                                                              cGCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTa                            <  1:6990917/93‑1 (MQ=255)

                                                              cGCTGTTTGACGGACGCCTGTTAAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGACATGACCATGTCGGTCTATCTGAGTATCAGCCTGACTa                            <  1:5910514/93‑1 (MQ=255)

                                                               gCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAt                           >  1:3998265/1‑93 (MQ=255)

                                                               gCTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAt                           <  1:2187205/93‑1 (MQ=255)

                                                                cTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtc                          >  2:8169582/1‑93 (MQ=255)

                                                                cTGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtc                          >  2:7170033/1‑93 (MQ=255)

                                                                cTGTTTGCCGGCACCGTGCTGAATCAGAC‑G‑GGTCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtc                          <  1:5644351/93‑1 (MQ=255)

                                                                 tGTTTGCCGGCACCGTGCTGAATCATACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         <  1:5350983/93‑1 (MQ=255)

                                                                 tGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGGCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         >  2:8230048/1‑93 (MQ=255)

                                                                 tGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         <  2:1272636/93‑1 (MQ=255)

                                                                 tGTTTGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAtct                         >  2:3316416/1‑93 (MQ=255)

                                                                    ttGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgc                      <  2:7243235/93‑1 (MQ=255)

                                                                     tGCCGGCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCgct                     <  1:4271196/93‑1 (MQ=255)

                                                                          gCACCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtga                <  2:7870124/93‑1 (MQ=255)

                                                                           gcccGTGCTGAATCAGAC‑GAGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTAGCAGCCTGACTATCTCGCTGCtgat               <  2:6812764/91‑1 (MQ=255)

                                                                            acggTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATGGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGTCTATCTCGCTGCtgatg              <  1:8462728/90‑1 (MQ=255)

                                                                            aCCGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgatg              >  1:2655908/1‑93 (MQ=255)

                                                                             cggTGCTGAATCAGAC‑G‑GGCCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgatga             <  1:2446475/91‑1 (MQ=255)

                                                                             ccGTGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCtgatga             >  2:7925498/1‑93 (MQ=255)

                                                                                tGCTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAata          <  2:4216902/93‑1 (MQ=255)

                                                                                  cTGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATc        <  1:1007300/93‑1 (MQ=255)

                                                                                  cTGAATCAGAC‑G‑GGCCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATc        <  2:1087522/93‑1 (MQ=255)

                                                                                   tGAATCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTCCTGATGAATATCt       <  1:5803529/93‑1 (MQ=255)

                                                                                       tCAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATaa   >  2:3906046/1‑93 (MQ=255)

                                                                                        cAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAc  >  1:179279/1‑93 (MQ=255)

                                                                                        cAGACGG‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAc  <  2:3212099/93‑1 (MQ=255)

GTATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑G‑GG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC  >  NC_000913/3418745‑3418924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 18 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGC  >  NC_000913/3418747‑3418930

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGG                                                                                             <  SRR4302541.7460215/94‑1 (MQ=60)

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCCGCAGCGCGCTGAATCAGAC‑GGG                                                                                             >  SRR4302541.1748218/1‑94 (MQ=60)

 TCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGG‑G                                                                                           <  SRR4302541.1906276/94‑1 (MQ=60)

  CTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGGgc                                                                                           <  SRR4302541.3293754/94‑3 (MQ=60)

     AACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAG                                                                                        <  SRR4302541.4857050/94‑1 (MQ=60)

     AACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAG                                                                                        >  SRR4302541.4733181/1‑94 (MQ=60)

     AACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAG                                                                                        >  SRR4302541.2851882/1‑94 (MQ=60)

      ACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGC                                                                                       >  SRR4302541.7100082/1‑94 (MQ=60)

      ACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGC                                                                                       >  SRR4302541.171039/1‑94 (MQ=60)

        ATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGTTTTCGCTGTTTGCCGGCACCGTGCTTAATCAGACGGGG‑CAAGCCA                                                                                     <  SRR4302541.5462269/94‑1 (MQ=60)

        ATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCA                                                                                     >  SRR4302541.345855/1‑94 (MQ=60)

        ATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCA                                                                                     <  SRR4302541.7060300/94‑1 (MQ=60)

        ATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCA                                                                                     >  SRR4302541.8799054/1‑94 (MQ=60)

        ATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCA                                                                                     <  SRR4302541.1275088/94‑1 (MQ=60)

         TCGTAAAAACCCCCTCTCTTGCCCCCGCTATCGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCAT                                                                                    <  SRR4302541.4919388/94‑1 (MQ=60)

          CGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATC                                                                                   <  SRR4302541.152817/94‑1 (MQ=60)

           GTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCG                                                                                  >  SRR4302541.7030257/1‑94 (MQ=60)

            TCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGA                                                                                 <  SRR4302541.490213/94‑1 (MQ=60)

             CAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAG                                                                                <  SRR4302541.3207108/94‑1 (MQ=60)

               AAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGAC                                                                              <  SRR4302541.7389842/94‑1 (MQ=60)

               AAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGAC                                                                              <  SRR4302541.6320720/94‑1 (MQ=60)

                AAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACG                                                                             >  SRR4302541.3597128/1‑94 (MQ=60)

                AAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACG                                                                             <  SRR4302541.8346668/94‑1 (MQ=60)

                 AACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGA                                                                            >  SRR4302541.3882188/1‑94 (MQ=60)

                  ACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGAT                                                                           >  SRR4302541.254650/1‑94 (MQ=60)

                  ACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGAT                                                                           >  SRR4302541.3352739/1‑94 (MQ=60)

                   CTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATA                                                                          >  SRR4302541.5397773/1‑94 (MQ=60)

                   CCCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATA                                                                          <  SRR4302541.1862897/94‑1 (MQ=60)

                    TCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAG                                                                         >  SRR4302541.5413680/1‑94 (MQ=60)

                    TCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAG                                                                         <  SRR4302541.83162/94‑1 (MQ=60)

                    CCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAG                                                                         <  SRR4302541.2786783/94‑1 (MQ=60)

                     CCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGC                                                                        >  SRR4302541.2218458/1‑94 (MQ=60)

                      CTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCC                                                                       >  SRR4302541.5261564/1‑94 (MQ=60)

                      CTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCC                                                                       <  SRR4302541.2223950/94‑1 (MQ=60)

                      CTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCC                                                                       >  SRR4302541.6554237/1‑94 (MQ=60)

                        CTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCAT                                                                     <  SRR4302541.7770364/94‑1 (MQ=60)

                        CTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCAT                                                                     <  SRR4302541.1149030/94‑1 (MQ=60)

                        CTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCAC                                                                     <  SRR4302541.7018493/94‑1 (MQ=60)

                         TCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATG                                                                    <  SRR4302541.5960805/94‑1 (MQ=60)

                          CTTTCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGA                                                                   <  SRR4302541.2527381/94‑1 (MQ=60)

                          CTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGA                                                                   <  SRR4302541.4074123/94‑1 (MQ=60)

                          CTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGA                                                                   >  SRR4302541.6866721/1‑94 (MQ=60)

                          CGTGCCGCCGCGATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGA                                                                   <  SRR4302541.8816348/94‑1 (MQ=60)

                           TTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGGCCAAGCCATCGAGACGATAGCCATGAC                                                                  >  SRR4302541.7353344/1‑94 (MQ=60)

                              CCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCAT                                                               <  SRR4302541.7563205/94‑1 (MQ=60)

                               CGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATATCCATGACCATG                                                              >  SRR4302541.3950917/1‑94 (MQ=60)

                               CGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATG                                                              >  SRR4302541.5472762/1‑94 (MQ=60)

                                 CCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTC                                                            >  SRR4302541.8337448/1‑94 (MQ=60)

                                 CCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTC                                                            <  SRR4302541.3865642/94‑1 (MQ=60)

                                  CGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCG                                                           >  SRR4302541.2716803/1‑94 (MQ=60)

                                   GCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAGTCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGG                                                          >  SRR4302541.7474836/1‑94 (MQ=60)

                                   GCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGG                                                          >  SRR4302541.5290715/1‑94 (MQ=60)

                                    CTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGT                                                         >  SRR4302541.634530/1‑94 (MQ=60)

                                    CTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGT                                                         >  SRR4302541.5804811/1‑94 (MQ=60)

                                    CTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGT                                                         >  SRR4302541.7568349/1‑94 (MQ=60)

                                     gagtgcctgtcccgagtTGGTTTCGCTGTTTGCCGGCACCGTGCTTAATCAGACGGGG‑CAAGCCATCGAGACGGTAGCCATGACCATGTCGGTC                                                        <  SRR4302541.1980612/77‑1 (MQ=60)

                                     TATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTC                                                        >  SRR4302541.6495662/1‑94 (MQ=60)

                                     TATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTC                                                        >  SRR4302541.4347003/1‑94 (MQ=60)

                                     TATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑AAAGCCATCGAGACGATAGCCATGACCATGTCGGTC                                                        <  SRR4302541.4194081/94‑1 (MQ=60)

                                      TTTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCT                                                       <  SRR4302541.1881443/94‑1 (MQ=60)

                                      ATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCT                                                       <  SRR4302541.4538543/94‑1 (MQ=60)

                                      ATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCT                                                       <  SRR4302541.303499/94‑1 (MQ=60)

                                         GGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATC                                                    >  SRR4302541.8492272/1‑94 (MQ=60)

                                         GGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATC                                                    <  SRR4302541.1100992/94‑1 (MQ=60)

                                         GGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATC                                                    >  SRR4302541.4901991/1‑94 (MQ=60)

                                          GCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCT                                                   >  SRR4302541.6732747/1‑94 (MQ=60)

                                          GCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCT                                                   >  SRR4302541.7561979/1‑94 (MQ=60)

                                          GCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCT                                                   <  SRR4302541.5140722/94‑1 (MQ=60)

                                          GCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCT                                                   <  SRR4302541.7161489/94‑1 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                  <  SRR4302541.8562442/94‑1 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                  >  SRR4302541.5503299/1‑94 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                  <  SRR4302541.8119327/94‑1 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                  <  SRR4302541.8216388/94‑1 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                  <  SRR4302541.1973992/94‑1 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTTGCCGGCACAGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                  <  SRR4302541.8562443/94‑1 (MQ=60)

                                           CTATCCCGATATGGTTTCGCTGTTGGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTG                                                  <  SRR4302541.3575617/94‑1 (MQ=60)

                                            TATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGA                                                 >  SRR4302541.3734991/1‑94 (MQ=60)

                                              TCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATT                                               >  SRR4302541.2571503/1‑94 (MQ=60)

                                                 CGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATC                                            >  SRR4302541.5267475/1‑94 (MQ=60)

                                                  GATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTTATTATCA                                           >  SRR4302541.4999910/1‑94 (MQ=60)

                                                  GATATGGTTTCGCTGTTTGACGGCACCGTGCTGCATCAGACGGGG‑CAAGCCATTGAGACGAGAGCCATGACTATGGCGGTCTATCTAATTAGCA                                           >  SRR4302541.4999911/1‑94 (MQ=50)

                                                   ATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAG                                          >  SRR4302541.266255/1‑94 (MQ=60)

                                                   ATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAG                                          >  SRR4302541.2285810/1‑94 (MQ=60)

                                                    TATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGC                                         >  SRR4302541.5069939/1‑94 (MQ=60)

                                                    TATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGC                                         >  SRR4302541.8704686/1‑94 (MQ=60)

                                                     ATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCC                                        <  SRR4302541.8270722/94‑1 (MQ=60)

                                                      TGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCT                                       <  SRR4302541.2428415/94‑1 (MQ=60)

                                                          tctccgctttcccgcgaccgttgTGAATCAGACGGGG‑CAAACCAGCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACT                                   <  SRR4302541.6620626/71‑1 (MQ=60)

                                                          TTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACT                                   <  SRR4302541.7442852/94‑1 (MQ=60)

                                                          TTCGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACT                                   <  SRR4302541.6620625/94‑1 (MQ=60)

                                                          TTCGCGGTTTTCCGGCTCCGTGCTGAAGCAGACGGGG‑AAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACT                                   <  SRR4302541.2378204/94‑1 (MQ=60)

                                                            cgctgtttgacggacgcctgttaAATCAGACGGGG‑CAAGCCATCGAGACGATAGACATGACCATGTCGGTCTATCTGAGTATCAGCCTGACTAT                                 <  SRR4302541.5970680/71‑1 (MQ=60)

                                                            CGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAT                                 >  SRR4302541.4037848/1‑94 (MQ=60)

                                                            CGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAT                                 <  SRR4302541.1242695/94‑1 (MQ=60)

                                                            CGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAT                                 >  SRR4302541.1157918/1‑94 (MQ=60)

                                                            CGCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTAT                                 <  SRR4302541.7065351/94‑1 (MQ=60)

                                                             GCTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATC                                <  SRR4302541.2208352/94‑1 (MQ=60)

                                                              CTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCT                               >  SRR4302541.913170/1‑94 (MQ=60)

                                                              CTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCT                               >  SRR4302541.5891143/1‑94 (MQ=60)

                                                              CTGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCT                               <  SRR4302541.5702031/94‑1 (MQ=60)

                                                              CTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGGTCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCT                               <  SRR4302541.5702030/94‑1 (MQ=60)

                                                               TGTTTGCCGGCACCGTGGTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                              <  SRR4302541.2600785/94‑1 (MQ=60)

                                                               TGTTTGCCGGCACCGTGCTGAATCATACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                              <  SRR4302541.5405972/94‑1 (MQ=60)

                                                               TGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                              >  SRR4302541.5018901/1‑94 (MQ=60)

                                                               TGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                              <  SRR4302541.7157623/94‑1 (MQ=60)

                                                               TGTTTGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTC                              <  SRR4302541.5405973/94‑1 (MQ=60)

                                                                   TGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTG                          <  SRR4302541.4313868/94‑1 (MQ=60)

                                                                   TGCCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTG                          <  SRR4302541.1974005/94‑1 (MQ=60)

                                                                    GCCGGCCCCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGC                         <  SRR4302541.6612893/94‑1 (MQ=60)

                                                                     CCGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCT                        <  SRR4302541.8735439/94‑1 (MQ=60)

                                                                      CGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGGGACGATAGCCATGACCATGTCGGTCTATCTGAGTATCAGCCTGACTATCTCGCTGCTG                       >  SRR4302541.1124742/1‑94 (MQ=60)

                                                                      CGGCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTG                       >  SRR4302541.846716/1‑94 (MQ=60)

                                                                        GCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGAT                     >  SRR4302541.5980588/1‑94 (MQ=60)

                                                                        GCACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGAT                     >  SRR4302541.4903386/1‑94 (MQ=60)

                                                                         CACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATG                    <  SRR4302541.5290756/94‑1 (MQ=60)

                                                                         CACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATG                    >  SRR4302541.2682329/1‑94 (MQ=60)

                                                                          CCCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGA                   <  SRR4302541.1901833/94‑1 (MQ=60)

                                                                          ACGGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATGGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGTCTATCTCGCTGCTGATGA                   <  SRR4302541.8554016/94‑1 (MQ=60)

                                                                          ACCGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGA                   <  SRR4302541.8158/94‑1 (MQ=60)

                                                                           CGGTGCTGAATCAGAC‑GGGCCAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAA                  <  SRR4302541.2470773/94‑1 (MQ=60)

                                                                            CGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAAT                 >  SRR4302541.4787106/1‑94 (MQ=60)

                                                                            CGTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAAT                 >  SRR4302541.5397899/1‑94 (MQ=60)

                                                                             GTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATA                >  SRR4302541.141500/1‑94 (MQ=60)

                                                                             GTGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATA                <  SRR4302541.2707739/94‑1 (MQ=60)

                                                                              TGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATAT               <  SRR4302541.1747466/94‑1 (MQ=60)

                                                                              TGCTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATAT               >  SRR4302541.6292408/1‑94 (MQ=60)

                                                                                CTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCT             <  SRR4302541.200248/94‑1 (MQ=60)

                                                                                CTGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCT             <  SRR4302541.1016639/94‑1 (MQ=60)

                                                                                 TGAATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTCCTGATGAATATCTA            <  SRR4302541.5862466/94‑1 (MQ=60)

                                                                                   AATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATA          <  SRR4302541.4771535/94‑1 (MQ=60)

                                                                                    ATCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAA         <  SRR4302541.5205841/94‑1 (MQ=60)

                                                                                     TCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC        <  SRR4302541.7936202/94‑1 (MQ=60)

                                                                                     TCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC        <  SRR4302541.691857/94‑1 (MQ=60)

                                                                                     TCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC        <  SRR4302541.7961241/94‑1 (MQ=60)

                                                                                     TCAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAAC        >  SRR4302541.180624/1‑94 (MQ=60)

                                                                                      CAGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACC       <  SRR4302541.3291469/94‑1 (MQ=60)

                                                                                       AGACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCG      <  SRR4302541.3786131/94‑1 (MQ=60)

                                                                                        GACGGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGC     >  SRR4302541.7382449/1‑94 (MQ=60)

                                                                                         acgGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCC    >  SRR4302541.1419260/4‑94 (MQ=60)

                                                                                         acgGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCC    >  SRR4302541.8329976/4‑94 (MQ=60)

                                                                                         acgGGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCC    >  SRR4302541.6603159/4‑94 (MQ=60)

                                                                                          G‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGC  >  SRR4302541.7371837/1‑94 (MQ=60)

                                                                                          G‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGC  >  SRR4302541.7371836/1‑94 (MQ=60)

                                                                                          G‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGC  >  SRR4302541.8376544/1‑94 (MQ=60)

                                                                                          G‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGC  <  SRR4302541.3720204/94‑1 (MQ=60)

ATCTCAACATCGTCAAAAACTCCTCTCTTGCCGCCGCTATTGGCTATCCCGATATGGTTTCGCTGTTTGCCGGCACCGTGCTGAATCAGAC‑GGG‑CAAGCCATCGAGACGATAGCCATGACCATGTCGGTCTATCTGATTATCAGCCTGACTATCTCGCTGCTGATGAATATCTATAACCGCCGC  >  NC_000913/3418747‑3418930

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 23 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`