Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,627,142 +G intergenic (‑24/+23) yhiH ← / ← yhiI putative ATP‑binding component of a transport system, fragment 1/putative membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,627,1401.G99.4% 1088.7 / ‑5.2 316intergenic (‑22/+25)yhiH/yhiIputative ATP‑binding component of a transport system, fragment 1/putative membrane protein
Reads supporting (aligned to +/- strand):  ref base . (0/0);  major base G (161/153);  minor base T (1/1);  total (162/154)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 378 total aligned reads displayed.

ACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑C‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA  >  NC_000913/3627048‑3627227
                                                                                              |                                                                                       
aCCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑c                                                                                          >  2:4330064/1‑93 (MQ=255)
aCCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑c                                                                                          >  1:3902003/1‑93 (MQ=255)
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑C‑g                                                                                        <  1:5676274/93‑1 (MQ=255)
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑C‑g                                                                                        <  2:1374457/93‑1 (MQ=255)
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑C‑g                                                                                        <  1:4988436/93‑1 (MQ=255)
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑C‑g                                                                                        <  2:7629028/93‑1 (MQ=255)
 ccATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑C‑g                                                                                        >  1:1244845/1‑93 (MQ=255)
  cATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑C‑gg                                                                                       <  1:4293937/93‑1 (MQ=255)
  cATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑C‑gg                                                                                       <  1:4221456/93‑1 (MQ=255)
    tACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGTGATAGG‑C‑gggg                                                                                     >  2:1693402/1‑91 (MQ=255)
    tACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑C‑ggga                                                                                     >  1:4505884/1‑91 (MQ=255)
    tACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑C‑ggga                                                                                     <  1:8032594/93‑3 (MQ=255)
          gggCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGaa                                                                                <  2:3640633/93‑1 (MQ=255)
           ggCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAAc                                                                               >  2:7401959/1‑93 (MQ=255)
           ggCCGGAATATCGAGAGTGATACTGGTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAAc                                                                               >  2:7821635/1‑93 (MQ=255)
            gCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAAcc                                                                              <  1:8175604/93‑1 (MQ=255)
             ccGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCa                                                                             <  2:4983714/93‑1 (MQ=255)
               ggAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGCTGCGCGACAGG‑CGGGACGGGAAGCAGt                                                                           >  2:5296423/1‑93 (MQ=255)
                gAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGAGCGACAGG‑CGGGACGGGCACCCGtt                                                                          >  2:4793108/1‑93 (MQ=255)
                  atatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTcc                                                                        <  2:4714696/93‑1 (MQ=255)
                  atatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTcc                                                                        >  2:4502293/1‑93 (MQ=255)
                   tatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGAGGGGAACCAGTTCCa                                                                       >  2:2341299/1‑93 (MQ=255)
                   tatCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCAAGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGCACCCGTTCCa                                                                       >  2:7496593/1‑93 (MQ=255)
                     tCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGa                                                                     >  1:6565274/1‑93 (MQ=255)
                      cGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGAt                                                                    <  2:6614484/93‑1 (MQ=255)
                      cGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGAt                                                                    >  2:5634675/1‑93 (MQ=255)
                       gagaGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATg                                                                   <  1:5234887/93‑1 (MQ=255)
                       gagaGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATg                                                                   >  2:5202081/1‑93 (MQ=255)
                        agagTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGGTCCAGATgg                                                                  >  2:2096042/1‑92 (MQ=255)
                          agTTATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCCCGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGt                                                                <  1:1980838/93‑1 (MQ=255)
                          agTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGt                                                                >  1:7491470/1‑93 (MQ=255)
                           gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTc                                                               <  1:6280454/93‑1 (MQ=255)
                           gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTc                                                               <  2:1040198/93‑1 (MQ=255)
                           gTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGCTGCGCGACAGGTG‑GGACATGTATCAGTGCCAGATGCGTc                                                               >  2:6142559/1‑93 (MQ=255)
                            tGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCa                                                              >  2:5667749/1‑93 (MQ=255)
                            tGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCa                                                              <  1:8499536/93‑1 (MQ=255)
                             gATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCAt                                                             <  2:8564137/93‑1 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAGCCAGTTCCAGATGCGTCAtt                                                            >  1:4692629/1‑93 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCAtt                                                            <  1:100618/93‑1 (MQ=255)
                              atatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCAtt                                                            >  2:1557202/1‑93 (MQ=255)
                               tatTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTg                                                           >  2:6533800/1‑93 (MQ=255)
                                atTGTGCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGc                                                          <  1:2914424/93‑1 (MQ=255)
                                 ttGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCg                                                         <  1:876421/93‑1 (MQ=255)
                                 ttGTTCAGCGAAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCg                                                         <  1:3821728/93‑1 (MQ=255)
                                   gatCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGc                                                       <  2:7284145/91‑1 (MQ=255)
                                   gTTCAGCGCAAGGGTTTTTCCATAATGCTGACTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGc                                                       <  1:7688044/93‑1 (MQ=255)
                                       aGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc                                                   <  1:2076775/93‑1 (MQ=255)
                                       aGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAcc                                                   >  2:6022340/1‑93 (MQ=255)
                                        gcgcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCt                                                  >  1:4827465/1‑93 (MQ=255)
                                         cgcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTc                                                 <  1:7247474/93‑1 (MQ=255)
                                          gcAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTca                                                <  1:7415394/93‑1 (MQ=255)
                                           cAACGTGTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcac                                               <  2:6311561/93‑1 (MQ=255)
                                           cAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcac                                               >  1:997260/1‑93 (MQ=255)
                                           cAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcac                                               >  1:639705/1‑93 (MQ=255)
                                            aaCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacc                                              <  2:7169592/93‑1 (MQ=255)
                                            aaCGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacc                                              >  1:6445015/1‑93 (MQ=255)
                                             aCGGTTTTTCCATAATGCGGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcacca                                             <  1:342673/93‑1 (MQ=255)
                                               ggTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTcaccaca                                           >  1:5451602/1‑92 (MQ=255)
                                               ggTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACg                                           <  2:5423240/93‑1 (MQ=255)
                                                gTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGa                                          <  2:4231985/93‑1 (MQ=255)
                                                 tttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAg                                         <  1:680882/93‑1 (MQ=255)
                                                  ttttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAgg                                        >  1:4161274/1‑93 (MQ=255)
                                                   tttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgt                                       <  1:3082230/93‑1 (MQ=255)
                                                    ttCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtc                                      >  2:1956589/1‑93 (MQ=255)
                                                     tCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCCCGAGgtcg                                     >  1:6034381/1‑93 (MQ=255)
                                                       cATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGgtcgtc                                   <  1:8379995/93‑1 (MQ=255)
                                                        aTAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCa                                  >  2:8789232/1‑93 (MQ=255)
                                                         tAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAg                                 >  2:8214372/1‑93 (MQ=255)
                                                          aaTGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAgg                                >  1:6520297/1‑93 (MQ=255)
                                                           aTGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGc                               >  1:2722050/1‑93 (MQ=255)
                                                           aTGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGc                               >  1:7706179/1‑93 (MQ=255)
                                                           aTGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGCCGTCAGGc                               >  1:5872242/1‑93 (MQ=255)
                                                            tGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGcc                              <  2:3960125/93‑1 (MQ=255)
                                                               tGGCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACg                           >  1:3150490/1‑93 (MQ=255)
                                                                ggCTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACgg                          >  1:953019/1‑93 (MQ=255)
                                                                  cTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGaa                        <  1:1718232/93‑1 (MQ=255)
                                                                  cTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGaa                        <  2:3299546/93‑1 (MQ=255)
                                                                  cTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGaa                        <  2:2713845/93‑1 (MQ=255)
                                                                  cTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGaa                        >  2:173982/1‑93 (MQ=255)
                                                                  cTCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGaa                        <  2:1899323/93‑1 (MQ=255)
                                                                   tCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCGTTGCGGCAACCTCACCACGAGGTCGTCAGGCCCCGGAAg                       >  1:6878659/1‑93 (MQ=255)
                                                                   tCACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAg                       >  2:8322909/1‑93 (MQ=255)
                                                                    cACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGt                      >  1:1391040/1‑93 (MQ=255)
                                                                    cACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGt                      >  1:2516652/1‑93 (MQ=255)
                                                                    cACGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGt                      <  2:1571600/93‑1 (MQ=255)
                                                                     aCGCCCGCCAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGtt                     <  1:6681723/93‑1 (MQ=255)
                                                                       gcccgccAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttct                   <  1:4334102/93‑1 (MQ=255)
                                                                         ccgccAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGttcttc                 >  2:8538184/1‑93 (MQ=255)
                                                                          cgccAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttca                >  2:2891152/1‑93 (MQ=255)
                                                                          cgccAGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttca                >  2:699923/1‑93 (MQ=255)
                                                                              aGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattca            <  1:78802/93‑1 (MQ=255)
                                                                              aGTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCttcattca            <  1:4028666/93‑1 (MQ=255)
                                                                               gTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCtttattcgc           >  2:5917754/1‑91 (MQ=255)
                                                                               gTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc           <  1:2361351/93‑1 (MQ=255)
                                                                               gTTGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAc           >  2:6430816/1‑93 (MQ=255)
                                                                                 tGCGCGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAccc         <  1:6197085/93‑1 (MQ=255)
                                                                                  gcgcGACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCg        >  2:4632924/1‑93 (MQ=255)
                                                                                  gagcgACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCg        <  2:5771184/91‑1 (MQ=255)
                                                                                    gcgACAGG‑CGGGAAGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCa      >  2:3481317/1‑93 (MQ=255)
                                                                                      gACAGG‑CGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCAcc    <  2:1290691/93‑1 (MQ=255)
                                                                                              |                                                                                       
ACCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGG‑C‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA  >  NC_000913/3627048‑3627227

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 204 total aligned reads displayed.
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGC  >  NC_000913/3627049‑3627235
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CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGTTTTGTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                               <  SRR4302541.8133695/94‑1 (MQ=60)
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                               >  SRR4302541.92853/1‑94 (MQ=60)
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                               <  SRR4302541.8133694/94‑1 (MQ=60)
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                               <  SRR4302541.5039635/94‑1 (MQ=60)
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                               <  SRR4302541.5734195/94‑1 (MQ=60)
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GG                                                                                               <  SRR4302541.467107/94‑1 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGG                                                                                              <  SRR4302541.4263473/94‑1 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGG                                                                                              <  SRR4302541.4336832/94‑1 (MQ=60)
 CATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGG                                                                                              >  SRR4302541.6894425/1‑94 (MQ=60)
 CATACAGCGGGCCGGAATATCGAAGGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCCCGCCCGCCAGTTGCGCGACATGC‑GGG                                                                                              <  SRR4302541.4336833/94‑1 (MQ=60)
  ATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGga                                                                                             >  SRR4302541.4551343/1‑92 (MQ=60)
  ATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGga                                                                                             <  SRR4302541.385984/94‑3 (MQ=60)
  ATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGga                                                                                             >  SRR4302541.918917/1‑92 (MQ=60)
   TACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgac                                                                                            <  SRR4302541.2722006/94‑4 (MQ=60)
   TACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgac                                                                                            <  SRR4302541.8120901/94‑4 (MQ=60)
   TACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgac                                                                                            >  SRR4302541.1983950/1‑91 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACG                                                                                            >  SRR4302541.1004538/1‑94 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACG                                                                                            <  SRR4302541.3337229/94‑1 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACG                                                                                            >  SRR4302541.6767836/1‑94 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACG                                                                                            <  SRR4302541.426579/94‑1 (MQ=60)
    ACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACG                                                                                            <  SRR4302541.8372896/94‑1 (MQ=60)
     CAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGG                                                                                           <  SRR4302541.3169412/94‑1 (MQ=60)
     CAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGG                                                                                           <  SRR4302541.7301821/94‑1 (MQ=60)
     CAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGG                                                                                           >  SRR4302541.2047896/1‑94 (MQ=60)
      AGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGG                                                                                          >  SRR4302541.6635368/1‑94 (MQ=60)
       GCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGA                                                                                         >  SRR4302541.4844451/1‑94 (MQ=60)
       GCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGA                                                                                         <  SRR4302541.1019830/94‑1 (MQ=60)
        CGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAA                                                                                        >  SRR4302541.2512480/1‑94 (MQ=60)
        CGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAA                                                                                        >  SRR4302541.4250713/1‑94 (MQ=60)
        CGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAA                                                                                        <  SRR4302541.3116264/94‑1 (MQ=60)
        CGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGggcgggaa                                                                                       >  SRR4302541.7560997/1‑86 (MQ=60)
        CGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgacgggga                                                                                       >  SRR4302541.3582383/1‑86 (MQ=60)
          GGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACC                                                                                      <  SRR4302541.43134/94‑1 (MQ=60)
           GCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCA                                                                                     <  SRR4302541.8023963/94‑1 (MQ=60)
           GCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCA                                                                                     <  SRR4302541.8264872/94‑1 (MQ=60)
            CCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAG                                                                                    >  SRR4302541.8093011/1‑94 (MQ=60)
            CCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAG                                                                                    <  SRR4302541.1024312/94‑1 (MQ=60)
             CGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGT                                                                                   <  SRR4302541.7413454/94‑1 (MQ=60)
             CGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGT                                                                                   <  SRR4302541.2357405/94‑1 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTT                                                                                  >  SRR4302541.2538072/1‑94 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTT                                                                                  >  SRR4302541.2151886/1‑94 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTT                                                                                  >  SRR4302541.4545991/1‑94 (MQ=60)
              GGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTT                                                                                  >  SRR4302541.4418360/1‑94 (MQ=60)
                AATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCC                                                                                <  SRR4302541.98531/94‑1 (MQ=60)
                AATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCC                                                                                <  SRR4302541.8534974/94‑1 (MQ=60)
                AATATCGAGAGCGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCC                                                                                >  SRR4302541.1616378/1‑94 (MQ=60)
                 ATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCA                                                                               >  SRR4302541.1374342/1‑94 (MQ=60)
                 ATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCA                                                                               <  SRR4302541.2408480/94‑1 (MQ=60)
                 ATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCA                                                                               >  SRR4302541.7931031/1‑94 (MQ=60)
                   ATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGA                                                                             <  SRR4302541.230546/94‑1 (MQ=60)
                   ATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGA                                                                             <  SRR4302541.8863914/94‑1 (MQ=60)
                   ATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGA                                                                             >  SRR4302541.6632423/1‑94 (MQ=60)
                    TCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGAT                                                                            <  SRR4302541.4217914/94‑1 (MQ=60)
                     CGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATG                                                                           >  SRR4302541.6568782/1‑94 (MQ=60)
                     CGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATG                                                                           >  SRR4302541.368542/1‑94 (MQ=60)
                     CGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATG                                                                           >  SRR4302541.6148802/1‑94 (MQ=60)
                      GAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGC                                                                          >  SRR4302541.6615893/1‑94 (MQ=60)
                      GAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGC                                                                          <  SRR4302541.5288823/94‑1 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCGGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATTCG                                                                         >  SRR4302541.4255647/1‑94 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCG                                                                         <  SRR4302541.5782057/94‑1 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCG                                                                         <  SRR4302541.6362582/94‑1 (MQ=60)
                       AGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGCTCCAGCTGCG                                                                         >  SRR4302541.4300845/1‑94 (MQ=60)
                        GAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGT                                                                        >  SRR4302541.8513803/1‑94 (MQ=60)
                        GAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGT                                                                        <  SRR4302541.8541502/94‑1 (MQ=60)
                        GAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGT                                                                        >  SRR4302541.7571586/1‑94 (MQ=60)
                        GAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGT                                                                        <  SRR4302541.6748407/94‑1 (MQ=60)
                         AGTTATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCCCGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTC                                                                       <  SRR4302541.1999973/94‑1 (MQ=60)
                         AGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTC                                                                       >  SRR4302541.6783074/1‑94 (MQ=60)
                         AGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTC                                                                       <  SRR4302541.6617526/94‑1 (MQ=60)
                          GTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCA                                                                      <  SRR4302541.6344599/94‑1 (MQ=60)
                           TGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCAT                                                                     <  SRR4302541.8591076/94‑1 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAGCCAGTTCCAGATGCGTCATT                                                                    >  SRR4302541.4740450/1‑94 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATT                                                                    >  SRR4302541.2084231/1‑94 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATT                                                                    <  SRR4302541.8102237/94‑1 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATT                                                                    <  SRR4302541.4044489/94‑1 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATT                                                                    <  SRR4302541.6325761/94‑1 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATT                                                                    <  SRR4302541.7605735/94‑1 (MQ=60)
                            GATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATT                                                                    <  SRR4302541.6798931/94‑1 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTG                                                                   <  SRR4302541.5829962/94‑1 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTG                                                                   <  SRR4302541.5448294/94‑1 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTG                                                                   <  SRR4302541.4010399/94‑1 (MQ=60)
                             ATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTG                                                                   <  SRR4302541.101470/94‑1 (MQ=60)
                              TATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGG                                                                  >  SRR4302541.2552203/1‑94 (MQ=60)
                              TATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGC                                                                  >  SRR4302541.5216580/1‑94 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCG                                                                 <  SRR4302541.3873731/94‑1 (MQ=60)
                               ATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCG                                                                 >  SRR4302541.8783223/1‑94 (MQ=60)
                               ATTGTGCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCG                                                                 <  SRR4302541.2943733/94‑1 (MQ=60)
                                TTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCGGTTCCAGATGCGTCATTGCGG                                                                >  SRR4302541.6056021/1‑94 (MQ=60)
                                TTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGG                                                                <  SRR4302541.884425/94‑1 (MQ=60)
                                TTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGG                                                                <  SRR4302541.6523057/94‑1 (MQ=60)
                                TTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGG                                                                <  SRR4302541.5204150/94‑1 (MQ=60)
                                TTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGG                                                                <  SRR4302541.5607592/94‑1 (MQ=60)
                                TTGTTCAGCGAAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGG                                                                <  SRR4302541.3860229/94‑1 (MQ=60)
                                 TGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGgacgggtaccagcttcagaatcgtcttttcggc                                                              >  SRR4302541.6608462/1‑61 (MQ=60)
                                  GTTCAGCGCAAGGGTTTTTCCATAATGCTGACTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCA                                                              <  SRR4302541.7769794/94‑1 (MQ=60)
                                  GTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCA                                                              <  SRR4302541.7528186/94‑1 (MQ=60)
                                  GTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGAA                                                              >  SRR4302541.2967142/1‑94 (MQ=60)
                                  GTGCAGCGCAAGGTTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCA                                                              <  SRR4302541.6280616/94‑1 (MQ=60)
                                   TTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAA                                                             <  SRR4302541.604700/94‑1 (MQ=60)
                                    TCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAC                                                            >  SRR4302541.7771651/1‑94 (MQ=60)
                                    TCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAAC                                                            >  SRR4302541.4640669/1‑94 (MQ=60)
                                      AGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAAGCAGTTTCAGATGCGTCATTGCGGtcactc                                                          >  SRR4302541.5161967/1‑88 (MQ=60)
                                      AGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCT                                                          >  SRR4302541.4876674/1‑94 (MQ=60)
                                      AGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCT                                                          <  SRR4302541.2096866/94‑1 (MQ=60)
                                       GCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTC                                                         <  SRR4302541.1007335/94‑1 (MQ=60)
                                        CGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCA                                                        <  SRR4302541.7324775/94‑1 (MQ=60)
                                         GCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCAC                                                       >  SRR4302541.645741/1‑94 (MQ=60)
                                         GCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCAC                                                       <  SRR4302541.3955076/94‑1 (MQ=60)
                                         GCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCAC                                                       <  SRR4302541.7494695/94‑1 (MQ=60)
                                         GCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCAC                                                       >  SRR4302541.1006543/1‑94 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                      >  SRR4302541.6510701/1‑94 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                      <  SRR4302541.7452318/94‑1 (MQ=60)
                                          CAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                      >  SRR4302541.4831388/1‑94 (MQ=60)
                                          CAACGGTTGTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACC                                                      <  SRR4302541.552913/94‑1 (MQ=60)
                                           AACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCC                                                     >  SRR4302541.2809368/1‑94 (MQ=60)
                                             CGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACG                                                   <  SRR4302541.5328366/94‑1 (MQ=60)
                                             CGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACA                                                   >  SRR4302541.5507537/1‑94 (MQ=60)
                                              GGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGA                                                  >  SRR4302541.8145488/1‑94 (MQ=60)
                                              GGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGA                                                  >  SRR4302541.7668958/1‑94 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGG                                                >  SRR4302541.4202456/1‑94 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGG                                                <  SRR4302541.7784050/94‑1 (MQ=60)
                                                TTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGG                                                <  SRR4302541.687306/94‑1 (MQ=60)
                                                 TTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGT                                               <  SRR4302541.5024976/94‑1 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                              <  SRR4302541.7326843/94‑1 (MQ=60)
                                                  TTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTC                                              <  SRR4302541.3113508/94‑1 (MQ=60)
                                                   TTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCCCGAGGTCG                                             >  SRR4302541.6096166/1‑94 (MQ=60)
                                                   TTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCG                                             <  SRR4302541.3391462/94‑1 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCA                                          <  SRR4302541.8470770/94‑1 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCA                                          >  SRR4302541.7919843/1‑94 (MQ=60)
                                                      CATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCA                                          <  SRR4302541.4611323/94‑1 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGCACCAGTTCCAGATGAGTCATTGCGGCAACCTCAACACGAcgccgccag                                         >  SRR4302541.8759486/1‑85 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                         >  SRR4302541.786264/1‑94 (MQ=60)
                                                       ATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAG                                         >  SRR4302541.4976332/1‑94 (MQ=60)
                                                        TAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                        >  SRR4302541.1307717/1‑94 (MQ=60)
                                                        TAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                        <  SRR4302541.6536657/94‑1 (MQ=60)
                                                        TAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                        >  SRR4302541.6586830/1‑94 (MQ=60)
                                                        TAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                        <  SRR4302541.5080097/94‑1 (MQ=60)
                                                        TAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                        >  SRR4302541.2000833/1‑94 (MQ=60)
                                                        TAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGG                                        >  SRR4302541.3715097/1‑94 (MQ=60)
                                                         AATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGC                                       >  SRR4302541.7788132/1‑94 (MQ=60)
                                                         AATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGC                                       >  SRR4302541.2749231/1‑94 (MQ=60)
                                                         AATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGCCGTCAGGC                                       >  SRR4302541.5931992/1‑94 (MQ=60)
                                                          ATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCC                                      >  SRR4302541.6374310/1‑94 (MQ=60)
                                                           agcgggccCACGCCCGCCAGTCGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCA                                     <  SRR4302541.6332004/86‑1 (MQ=60)
                                                             CTGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACG                                   >  SRR4302541.3182290/1‑94 (MQ=60)
                                                              TGGCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGG                                  >  SRR4302541.962043/1‑94 (MQ=60)
                                                               GGCTCGCGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGA                                 <  SRR4302541.4497054/94‑1 (MQ=60)
                                                                GCTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAA                                >  SRR4302541.6903337/1‑94 (MQ=60)
                                                                 CTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCGTTGCGGCAACCTCACCACGAGGTCGTCAGGCCCCGGAAG                               >  SRR4302541.6952104/1‑94 (MQ=60)
                                                                 CTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAG                               <  SRR4302541.4857490/94‑1 (MQ=60)
                                                                 CTCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAG                               <  SRR4302541.1735037/94‑1 (MQ=60)
                                                                  TCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGT                              >  SRR4302541.1404676/1‑94 (MQ=60)
                                                                  TCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGT                              >  SRR4302541.2541986/1‑94 (MQ=60)
                                                                  TCACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGT                              >  SRR4302541.1971138/1‑94 (MQ=60)
                                                                    CCGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTC                            <  SRR4302541.6386931/94‑1 (MQ=60)
                                                                    ACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTC                            <  SRR4302541.6750827/94‑1 (MQ=60)
                                                                    ACGCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTC                            <  SRR4302541.7252953/94‑1 (MQ=60)
                                                                     CGCCCGCCAGTTGCGAGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCACCCTCACCACGAGGTCGTCAGGCCACGGAAGTTCT                           <  SRR4302541.6890578/94‑1 (MQ=60)
                                                                      GCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTT                          <  SRR4302541.4377374/94‑1 (MQ=60)
                                                                      GCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTT                          <  SRR4302541.838667/94‑1 (MQ=60)
                                                                      GCCCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTT                          >  SRR4302541.4139197/1‑94 (MQ=60)
                                                                        CCGCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCA                        <  SRR4302541.6499487/94‑1 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                      <  SRR4302541.4134089/94‑1 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                      >  SRR4302541.4085486/1‑94 (MQ=60)
                                                                          GCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                      >  SRR4302541.6211384/1‑94 (MQ=60)
                                                                          CCCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATT                      <  SRR4302541.7529188/94‑1 (MQ=60)
                                                                           CCAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTC                     >  SRR4302541.278052/1‑94 (MQ=60)
                                                                            CAGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCA                    <  SRR4302541.2960440/94‑1 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                   >  SRR4302541.435554/1‑94 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                   <  SRR4302541.4068471/94‑1 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                   >  SRR4302541.1883611/1‑94 (MQ=60)
                                                                             AGTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCAC                   <  SRR4302541.79477/94‑1 (MQ=60)
                                                                              GTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACC                  <  SRR4302541.2384364/94‑1 (MQ=60)
                                                                              GTTGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACC                  <  SRR4302541.7506620/94‑1 (MQ=60)
                                                                               ggcCGCGACAGGCGGGGCGGGAACCAGTGCCAGGTGCGTCATTGCGGCCACCTCACCACGAGGTCGGCAGGCCACGGAAGTTCTTCATTCACCC                 <  SRR4302541.1515027/91‑1 (MQ=60)
                                                                                TGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCG                <  SRR4302541.5667015/94‑1 (MQ=60)
                                                                                TGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCG                <  SRR4302541.2882815/94‑1 (MQ=60)
                                                                                TGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCG                <  SRR4302541.6260404/94‑1 (MQ=60)
                                                                                TGCGCGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCG                <  SRR4302541.2698754/94‑1 (MQ=60)
                                                                                    CGACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACC            >  SRR4302541.5814216/1‑94 (MQ=60)
                                                                                     GACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCC           >  SRR4302541.8739891/1‑94 (MQ=60)
                                                                                     GACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCC           <  SRR4302541.6156832/94‑1 (MQ=60)
                                                                                     GACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCC           <  SRR4302541.6726023/94‑1 (MQ=60)
                                                                                      ACAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCA          >  SRR4302541.776223/1‑94 (MQ=60)
                                                                                       CAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAC         >  SRR4302541.3449346/1‑94 (MQ=60)
                                                                                       CAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAC         <  SRR4302541.4343568/94‑1 (MQ=60)
                                                                                       CAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAC         >  SRR4302541.918024/1‑94 (MQ=60)
                                                                                       CAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAC         <  SRR4302541.3340348/94‑1 (MQ=60)
                                                                                       CAGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGCCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCAC         >  SRR4302541.2250387/1‑94 (MQ=60)
                                                                                        AGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACG        >  SRR4302541.3189031/1‑94 (MQ=60)
                                                                                        AGGCGGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACG        >  SRR4302541.526603/1‑94 (MQ=60)
                                                                                          gcgGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCT      <  SRR4302541.3535995/91‑1 (MQ=60)
                                                                                          gcgGGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCT      <  SRR4302541.6002204/91‑1 (MQ=60)
                                                                                           G‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCGCGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTAC    >  SRR4302541.8611749/1‑94 (MQ=60)
                                                                                           G‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGTTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTAC    >  SRR4302541.8842355/1‑94 (MQ=60)
                                                                                           G‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTAC    >  SRR4302541.7155403/1‑94 (MQ=60)
                                                                                             GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACG   <  SRR4302541.3452164/94‑1 (MQ=60)
                                                                                             GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACG   <  SRR4302541.1702207/94‑1 (MQ=60)
                                                                                             GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACG   <  SRR4302541.434566/94‑1 (MQ=60)
                                                                                              GACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGC  >  SRR4302541.5658285/1‑94 (MQ=60)
                                                                                              |                                                                                             
CCATACAGCGGGCCGGAATATCGAGAGTGATATTGTTCAGCGCAACGGTTTTTCCATAATGCTGGCTCACGCCCGCCAGTTGCGCGACAGGC‑GGACGGGAACCAGTTCCAGATGCGTCATTGCGGCAACCTCACCACGAGGTCGTCAGGCCACGGAAGTTCTTCATTCACCCGCACCCACGCTACGC  >  NC_000913/3627049‑3627235

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑