Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,654,259 T→C T38A (ACC→GCC)  hdeA ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,654,2590TC99.7% 1327.8 / ‑6.0 388T38A (ACC→GCC) hdeAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (190/196);  total (191/197)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 399 total aligned reads displayed.

CTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGTCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAA  >  NC_000913/3654169‑3654348
                                                                                          |                                                                                         
cttctGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGccc                                                                                         >  2:6900947/1‑93 (MQ=255)
cttctGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGccc                                                                                         >  2:716774/1‑93 (MQ=255)
cttctGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGccc                                                                                         <  2:3391531/93‑1 (MQ=255)
  tctGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAg                                                                                       <  2:648673/93‑1 (MQ=255)
  tctGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAg                                                                                       >  2:6763661/1‑93 (MQ=255)
  tctGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAg                                                                                       >  2:2201649/1‑93 (MQ=255)
   ttGGTTGATCTTTTTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAgg                                                                                      <  1:4208561/92‑1 (MQ=255)
   ctGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAgg                                                                                      >  1:3693446/1‑93 (MQ=255)
    tGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGa                                                                                     <  2:4934864/93‑1 (MQ=255)
    tGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGa                                                                                     <  1:5614168/93‑1 (MQ=255)
     ggTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAg                                                                                    <  1:7012362/93‑1 (MQ=255)
      gTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGt                                                                                   >  2:7500648/1‑93 (MQ=255)
       tttATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGtt                                                                                  <  2:3689312/93‑1 (MQ=255)
         tATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGa                                                                                >  2:3930982/1‑93 (MQ=255)
           tCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGAcc                                                                              >  1:6134193/1‑93 (MQ=255)
            cTTTGTTCTCCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCg                                                                             <  1:4414862/93‑1 (MQ=255)
             tttGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCgg                                                                            >  1:2676377/1‑93 (MQ=255)
              ttgttgTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGt                                                                           >  2:5502565/1‑93 (MQ=255)
               tgttgtTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGtt                                                                          <  1:4317672/93‑1 (MQ=255)
                gttgttCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGttt                                                                         >  2:2074255/1‑93 (MQ=255)
                gttgttCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGttt                                                                         <  1:6199588/93‑1 (MQ=255)
                   gttCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGtttttt                                                                      >  2:1820817/1‑93 (MQ=255)
                   gttCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGtttttt                                                                      <  1:1488825/93‑1 (MQ=255)
                   gttCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGgttttt                                                                      >  1:289153/1‑93 (MQ=255)
                      cAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGtt                                                                   >  1:2753831/1‑93 (MQ=255)
                      cAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGtt                                                                   >  2:7638215/1‑93 (MQ=255)
                         cgcTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATc                                                                <  1:3975254/93‑1 (MQ=255)
                         cgcTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATc                                                                <  1:8342440/93‑1 (MQ=255)
                          gcTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCa                                                               >  2:1364084/1‑93 (MQ=255)
                          gcTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGGCCACAGCCAGGAAATCTTCAAAGGCCCAGGAGTTGACCGGTTTTTTGTTATCa                                                               <  1:5386607/93‑1 (MQ=255)
                           cTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAg                                                              >  1:6389621/1‑93 (MQ=255)
                           cTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAg                                                              <  2:901768/93‑1 (MQ=255)
                           cTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAg                                                              >  2:6085671/1‑93 (MQ=255)
                           cTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAg                                                              <  1:4845368/93‑1 (MQ=255)
                            ttCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAgc                                                             <  2:2123236/93‑1 (MQ=255)
                            ttCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAgc                                                             >  2:8731308/1‑93 (MQ=255)
                            ttCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAgc                                                             >  1:822032/1‑93 (MQ=255)
                             tCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAgct                                                            <  1:1825624/93‑1 (MQ=255)
                                tcAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCACAGGAGTTGACCGGTTTTTTGTTATCAgatgct                                                         <  1:725344/92‑1 (MQ=255)
                                gCAAAACCAACTGCTGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAgctgct                                                         <  1:7501295/93‑1 (MQ=255)
                                gCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAgctgct                                                         >  1:7776157/1‑93 (MQ=255)
                                  aaaaCCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCttt                                                       >  2:6644816/1‑93 (MQ=255)
                                   aaaCCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCa                                                                                        <  2:6177767/59‑1 (MQ=255)
                                   aaaCCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCa                                                                                        >  1:4550605/1‑59 (MQ=255)
                                   aaaCCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCtttt                                                      >  1:6548097/1‑93 (MQ=255)
                                    aaCCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCttttt                                                     >  2:1080461/1‑93 (MQ=255)
                                    aaCCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCttttt                                                     >  1:6665202/1‑93 (MQ=255)
                                     aCCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTg                                                    <  2:4266228/93‑1 (MQ=255)
                                      ccAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTgc                                                   >  1:1245646/1‑93 (MQ=255)
                                      ccAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTgc                                                   >  1:3179269/1‑93 (MQ=255)
                                           tGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATc                                              >  2:4733497/1‑93 (MQ=255)
                                            gCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATcc                                             <  2:8351960/93‑1 (MQ=255)
                                               gTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCt                                          >  1:7943438/1‑93 (MQ=255)
                                               gTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCt                                          >  2:5005702/1‑93 (MQ=255)
                                               gTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGATGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCt                                          >  2:785269/1‑93 (MQ=255)
                                                ttGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTg                                         <  1:51428/93‑1 (MQ=255)
                                                  ggCTGGAAGGATTCGTCCACAGCCAGTAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCa                                       <  2:2745524/93‑1 (MQ=255)
                                                    cTGGAAGGATTTGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCAtt                                     >  1:1396544/1‑93 (MQ=255)
                                                    cTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCAtt                                     <  1:1671644/93‑1 (MQ=255)
                                                     tGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTg                                    >  1:7179846/1‑93 (MQ=255)
                                                      ggAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGc                                   >  2:4441678/1‑93 (MQ=255)
                                                      ggAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGc                                   >  2:5756009/1‑93 (MQ=255)
                                                       gAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCt                                  <  2:158074/93‑1 (MQ=255)
                                                        aaGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTc                                 <  2:5309825/93‑1 (MQ=255)
                                                         aGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTca                                >  1:6853543/1‑93 (MQ=255)
                                                         aGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTca                                >  1:5794337/1‑93 (MQ=255)
                                                         aGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTca                                >  2:7270588/1‑93 (MQ=255)
                                                          ggATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTcac                               >  2:2529631/1‑93 (MQ=255)
                                                           gATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGGTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTcaca                              >  2:3072279/1‑93 (MQ=255)
                                                             ttCGTCCACAGCCAGGAAATCTTCACAGGCCCATGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAAc                            >  1:3232761/1‑93 (MQ=255)
                                                                gTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTgg                         >  1:495663/1‑93 (MQ=255)
                                                                 tCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGc                        >  1:1104596/1‑93 (MQ=255)
                                                                 tCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGc                        <  2:5600420/93‑1 (MQ=255)
                                                                  ccACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCa                       <  1:3867738/93‑1 (MQ=255)
                                                                  ccACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCa                       >  1:2599153/1‑93 (MQ=255)
                                                                   cacaGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCag                      <  1:7323812/93‑1 (MQ=255)
                                                                      aGCCAGGAAATCTTCACAGGCCCAGTAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCagaag                   <  2:4076022/93‑1 (MQ=255)
                                                                       gCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCagaaga                  >  2:344589/1‑93 (MQ=255)
                                                                       gCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCagaaga                  <  2:2657297/93‑1 (MQ=255)
                                                                       gCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCagaaga                  <  2:5817997/93‑1 (MQ=255)
                                                                       gCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCagaaga                  >  2:8495210/1‑93 (MQ=255)
                                                                         cAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGaagaag                <  2:419877/93‑1 (MQ=255)
                                                                         cAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGaagaag                <  1:8805919/93‑1 (MQ=255)
                                                                         cAGGAAATCCTCACAGGCCCAGGGGTTGACCGGTTTTTTGGTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCCCACAACCGGCAGaagaag                <  2:4330504/93‑1 (MQ=255)
                                                                          aGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGc               <  2:4602433/93‑1 (MQ=255)
                                                                          aGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGc               >  2:1937834/1‑93 (MQ=255)
                                                                           ggAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCa              >  1:3519550/1‑93 (MQ=255)
                                                                           ggAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCa              >  2:8042842/1‑93 (MQ=255)
                                                                           ggAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCa              <  2:823270/93‑1 (MQ=255)
                                                                           ggAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCa              >  1:8716715/1‑93 (MQ=255)
                                                                           ggAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCa              >  1:8651609/1‑93 (MQ=255)
                                                                            gAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAg             <  1:2029861/93‑1 (MQ=255)
                                                                             aaaTCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGa            <  1:6032937/93‑1 (MQ=255)
                                                                              aaTCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGac           <  2:126839/93‑1 (MQ=255)
                                                                                tCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGAAGacca         >  1:5468098/1‑93 (MQ=255)
                                                                                 cTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGaccac        <  1:761103/93‑1 (MQ=255)
                                                                                      caGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGa   <  2:7004847/93‑1 (MQ=255)
                                                                                       aGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGaa  <  1:5009675/93‑1 (MQ=255)
                                                                                       aGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGaa  <  1:8097985/93‑1 (MQ=255)
                                                                                       aGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGaa  <  2:2363708/93‑1 (MQ=255)
                                                                                          |                                                                                         
CTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGTCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAA  >  NC_000913/3654169‑3654348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 214 total aligned reads displayed.
CATCTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGTCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAAC  >  NC_000913/3654166‑3654352
                                                                                             |                                                                                             
CATCTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGC                                                                                               <  SRR4302541.1165158/94‑1 (MQ=60)
CATCTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGC                                                                                               >  SRR4302541.5380953/1‑94 (MQ=60)
CATCTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGC                                                                                               <  SRR4302541.5504442/94‑1 (MQ=60)
CATCTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGC                                                                                               >  SRR4302541.8152880/1‑94 (MQ=60)
 ATCTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCC                                                                                              >  SRR4302541.5527448/1‑94 (MQ=60)
 ATCTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCC                                                                                              <  SRR4302541.2872063/94‑1 (MQ=60)
 ATCTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCC                                                                                              >  SRR4302541.7044565/1‑94 (MQ=60)
 ATCTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTAGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCC                                                                                              <  SRR4302541.4900712/94‑1 (MQ=60)
  TCTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCC                                                                                             >  SRR4302541.4939518/1‑94 (MQ=60)
   CTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACTGCCAGGAAATCTTCACAGGCCCA                                                                                            <  SRR4302541.6622468/94‑1 (MQ=60)
   CTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCA                                                                                            >  SRR4302541.7586559/1‑94 (MQ=60)
   CTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCA                                                                                            <  SRR4302541.6609235/94‑1 (MQ=60)
     TCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGG                                                                                          >  SRR4302541.3730804/1‑94 (MQ=60)
     TCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGCTCCAGG                                                                                          >  SRR4302541.2035652/1‑94 (MQ=60)
      TTGGTTGATCTTTTTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGA                                                                                         <  SRR4302541.4250411/94‑1 (MQ=60)
      CTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGA                                                                                         <  SRR4302541.4188440/94‑1 (MQ=60)
      CTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGA                                                                                         <  SRR4302541.4250412/94‑1 (MQ=60)
      CTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGA                                                                                         <  SRR4302541.4120103/94‑1 (MQ=60)
       TGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAG                                                                                        <  SRR4302541.5671634/94‑1 (MQ=60)
       TGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAG                                                                                        >  SRR4302541.4671476/1‑94 (MQ=60)
        GGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGT                                                                                       <  SRR4302541.7086948/94‑1 (MQ=60)
         GGTTATCTTTGTTTTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTT                                                                                      <  SRR4302541.2666340/94‑1 (MQ=60)
           TTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGA                                                                                    >  SRR4302541.8243218/1‑94 (MQ=60)
            TATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGAC                                                                                   >  SRR4302541.4606836/1‑94 (MQ=60)
            TATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGAC                                                                                   >  SRR4302541.720195/1‑94 (MQ=60)
             ATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACC                                                                                  >  SRR4302541.6196829/1‑94 (MQ=60)
             ATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACC                                                                                  <  SRR4302541.2073219/94‑1 (MQ=60)
             ATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACC                                                                                  >  SRR4302541.6196830/1‑94 (MQ=60)
             ATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACC                                                                                  <  SRR4302541.7532671/94‑1 (MQ=60)
              TCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCG                                                                                 <  SRR4302541.8252825/94‑1 (MQ=60)
              TCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCG                                                                                 <  SRR4302541.4277320/94‑1 (MQ=60)
              TCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCG                                                                                 <  SRR4302541.2791221/94‑1 (MQ=60)
              TCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCATCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCG                                                                                 <  SRR4302541.7336574/94‑1 (MQ=60)
               CTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGG                                                                                >  SRR4302541.7544407/1‑94 (MQ=60)
               CTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGG                                                                                >  SRR4302541.2702958/1‑94 (MQ=60)
               CTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGG                                                                                <  SRR4302541.5543631/94‑1 (MQ=60)
               CTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGG                                                                                >  SRR4302541.5369168/1‑94 (MQ=60)
               CTTTGTTCTCCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGG                                                                                <  SRR4302541.4459124/94‑1 (MQ=60)
                TTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAATTCTTCACAGGCCCAGCAGTTGACCGGT                                                                               >  SRR4302541.6572545/1‑94 (MQ=60)
                TTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGT                                                                               >  SRR4302541.8079457/1‑94 (MQ=60)
                  TGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTT                                                                             <  SRR4302541.4360785/94‑1 (MQ=60)
                  TGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTT                                                                             >  SRR4302541.5618745/1‑94 (MQ=60)
                   GTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTT                                                                            <  SRR4302541.6262936/94‑1 (MQ=60)
                   GTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTT                                                                            >  SRR4302541.8355706/1‑94 (MQ=60)
                    TTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTT                                                                           <  SRR4302541.3927044/94‑1 (MQ=60)
                    TTGTTCAGCGCTTCAGCAAAACCAACTGCAGTGGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTT                                                                           <  SRR4302541.7372575/94‑1 (MQ=60)
                     TGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGGTTTTT                                                                          >  SRR4302541.291652/1‑94 (MQ=60)
                      GTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTG                                                                         <  SRR4302541.2010582/94‑1 (MQ=60)
                      GTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTG                                                                         <  SRR4302541.1503488/94‑1 (MQ=60)
                      GTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTG                                                                         <  SRR4302541.671630/94‑1 (MQ=60)
                       TTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGT                                                                        <  SRR4302541.4567050/94‑1 (MQ=60)
                       TTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGT                                                                        >  SRR4302541.1455349/1‑94 (MQ=60)
                        TCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTT                                                                       >  SRR4302541.8913042/1‑94 (MQ=60)
                        TCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTT                                                                       >  SRR4302541.2781499/1‑94 (MQ=60)
                        TCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTT                                                                       >  SRR4302541.8740693/1‑94 (MQ=60)
                         CAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTA                                                                      >  SRR4302541.503900/1‑94 (MQ=60)
                          AGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTAT                                                                     >  SRR4302541.6132411/1‑94 (MQ=60)
                          AGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTAT                                                                     <  SRR4302541.2220846/94‑1 (MQ=60)
                          AGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTAT                                                                     >  SRR4302541.5129745/1‑94 (MQ=60)
                           GCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATC                                                                    <  SRR4302541.6710909/94‑1 (MQ=60)
                            CGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCA                                                                   <  SRR4302541.6194031/94‑1 (MQ=60)
                            CGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCA                                                                   >  SRR4302541.7814648/1‑94 (MQ=60)
                            CGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCA                                                                   <  SRR4302541.8432840/94‑1 (MQ=60)
                            CGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCA                                                                   <  SRR4302541.4014695/94‑1 (MQ=60)
                            CGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCA                                                                   >  SRR4302541.3241535/1‑94 (MQ=60)
                            CGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCA                                                                   >  SRR4302541.3915320/1‑94 (MQ=60)
                             GCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAACTCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAG                                                                  <  SRR4302541.326756/94‑1 (MQ=60)
                             GCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAG                                                                  <  SRR4302541.4051177/94‑1 (MQ=60)
                             GCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAG                                                                  <  SRR4302541.7060641/94‑1 (MQ=60)
                             GCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAG                                                                  >  SRR4302541.6454859/1‑94 (MQ=60)
                             GCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAG                                                                  <  SRR4302541.7765191/94‑1 (MQ=60)
                             GCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAG                                                                  <  SRR4302541.5441901/94‑1 (MQ=60)
                             GCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAG                                                                  <  SRR4302541.8081670/94‑1 (MQ=60)
                             ACTTCAGCAAACCCAACTGCAGTTGGCTGGAGGAATGCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGCCCGGTTTTTTGTTATCAG                                                                  <  SRR4302541.4883851/94‑1 (MQ=60)
                              CTTCAGCAAAACCAACTGCAGTTTGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGC                                                                 <  SRR4302541.2650044/94‑1 (MQ=60)
                              CTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGC                                                                 <  SRR4302541.5716042/94‑1 (MQ=60)
                              CTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGC                                                                 <  SRR4302541.4894758/94‑1 (MQ=60)
                              CTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGC                                                                 <  SRR4302541.1497104/94‑1 (MQ=60)
                              CTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGC                                                                 >  SRR4302541.829513/1‑94 (MQ=60)
                                tccgccaacccctctgccgttggctggcgggcTTCGTCCCCAGCCAGGAAATCTTCCCGGGCCCAGGGGTTGACCGGTTTTTTGTTATCCGCTG                                                               <  SRR4302541.6782596/62‑1 (MQ=28)
                                TCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTG                                                               <  SRR4302541.1843335/94‑1 (MQ=60)
                                TCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTG                                                               <  SRR4302541.2799463/94‑1 (MQ=60)
                                  AGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCT                                                             >  SRR4302541.7858884/1‑94 (MQ=60)
                                  AGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCT                                                             >  SRR4302541.7683197/1‑94 (MQ=60)
                                  AGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCT                                                             >  SRR4302541.2774963/1‑94 (MQ=60)
                                  AGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCT                                                             <  SRR4302541.296026/94‑1 (MQ=60)
                                   TCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCACAGGAGTTGACCGGTTTTTTGTTATCAGATGCTT                                                            <  SRR4302541.732221/94‑1 (MQ=60)
                                   GCAAAACCAACTGCTGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTT                                                            <  SRR4302541.7581481/94‑1 (MQ=60)
                                   GCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTT                                                            <  SRR4302541.7991915/94‑1 (MQ=60)
                                   GCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTT                                                            >  SRR4302541.7208654/1‑94 (MQ=60)
                                    CAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTT                                                           >  SRR4302541.4896499/1‑94 (MQ=60)
                                     AAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTT                                                          <  SRR4302541.4007795/94‑1 (MQ=60)
                                     AAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTT                                                          >  SRR4302541.6615000/1‑94 (MQ=60)
                                     AAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTT                                                          >  SRR4302541.6799022/1‑94 (MQ=60)
                                      AAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTT                                                         >  SRR4302541.6733607/1‑94 (MQ=60)
                                      AAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTT                                                         >  SRR4302541.8848864/1‑94 (MQ=60)
                                       AACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTG                                                        >  SRR4302541.1114978/1‑94 (MQ=60)
                                       AACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTG                                                        <  SRR4302541.2953904/94‑1 (MQ=60)
                                        ACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTTACCGGTTTTTTGTTATCAGCTGCTTTTTGC                                                       <  SRR4302541.6226230/94‑1 (MQ=60)
                                        ACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGC                                                       >  SRR4302541.1257991/1‑94 (MQ=60)
                                        ACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGC                                                       >  SRR4302541.3211388/1‑94 (MQ=60)
                                         CCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCG                                                      <  SRR4302541.4424530/94‑1 (MQ=60)
                                          CAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGC                                                     >  SRR4302541.879802/1‑94 (MQ=60)
                                          CAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGC                                                     >  SRR4302541.6068733/1‑94 (MQ=60)
                                           AACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTG‑GCAT                                                   >  SRR4302541.8786429/1‑94 (MQ=60)
                                            ACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCAT                                                   >  SRR4302541.451387/1‑94 (MQ=60)
                                             CTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATC                                                  >  SRR4302541.5506440/1‑94 (MQ=60)
                                              TCCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCCCATCC                                                 <  SRR4302541.6909753/94‑1 (MQ=60)
                                               GCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCG                                                <  SRR4302541.4716197/94‑1 (MQ=60)
                                               GCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCG                                                <  SRR4302541.5145985/94‑1 (MQ=60)
                                               GCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCACATCCG                                                <  SRR4302541.3257859/94‑1 (MQ=60)
                                                CAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGC                                               <  SRR4302541.1579110/94‑1 (MQ=60)
                                                 AGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCT                                              >  SRR4302541.8030933/1‑94 (MQ=60)
                                                 AGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCT                                              <  SRR4302541.6929381/94‑1 (MQ=60)
                                                 AGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCT                                              >  SRR4302541.5691204/1‑94 (MQ=60)
                                                 AGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCT                                              >  SRR4302541.3318671/1‑94 (MQ=60)
                                                 AGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCT                                              >  SRR4302541.3887569/1‑94 (MQ=60)
                                                  GTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTG                                             <  SRR4302541.2817374/94‑1 (MQ=60)
                                                   TTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGC                                            <  SRR4302541.51831/94‑1 (MQ=60)
                                                    TGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCA                                           >  SRR4302541.2128429/1‑94 (MQ=60)
                                                      GCTGGAAGGATTTGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATT                                         >  SRR4302541.1410213/1‑94 (MQ=60)
                                                      GCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATT                                         <  SRR4302541.3647954/94‑1 (MQ=60)
                                                       CTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTG                                        >  SRR4302541.2711727/1‑94 (MQ=60)
                                                       CTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTG                                        <  SRR4302541.1296488/94‑1 (MQ=60)
                                                       CTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTG                                        <  SRR4302541.153357/94‑1 (MQ=60)
                                                       CTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTG                                        <  SRR4302541.1688116/94‑1 (MQ=60)
                                                       CTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTG                                        >  SRR4302541.7256429/1‑94 (MQ=60)
                                                        TGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGC                                       >  SRR4302541.3263578/1‑94 (MQ=60)
                                                        TGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGC                                       >  SRR4302541.5894219/1‑94 (MQ=60)
                                                        TGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGC                                       >  SRR4302541.7603531/1‑94 (MQ=60)
                                                         GGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCT                                      >  SRR4302541.6329508/1‑94 (MQ=60)
                                                         GGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCT                                      <  SRR4302541.1732614/94‑1 (MQ=60)
                                                         GGAAGGATTAGTCCACAGACAGGAAATATTAACAGGCCCAGGGGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCGTCCGCTGGATTGCT                                      >  SRR4302541.5822982/1‑94 (MQ=60)
                                                           AAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCA                                    >  SRR4302541.5853211/1‑94 (MQ=60)
                                                           AAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCA                                    >  SRR4302541.1402396/1‑94 (MQ=60)
                                                           AAGGATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCA                                    >  SRR4302541.6926797/1‑94 (MQ=60)
                                                               ATTCGTCCACAGCCAGGAAATCTTCACAGGCCCATGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAAC                                >  SRR4302541.3265402/1‑94 (MQ=60)
                                                               ATTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAAC                                >  SRR4302541.8133580/1‑94 (MQ=60)
                                                               ATTCGTCCACAGCAAGGAACTCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAAC                                <  SRR4302541.7916426/94‑1 (MQ=60)
                                                                TTCGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACT                               <  SRR4302541.6321714/94‑1 (MQ=60)
                                                                  CGTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGG                             >  SRR4302541.500336/1‑94 (MQ=60)
                                                                   GTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGC                            >  SRR4302541.5263406/1‑94 (MQ=60)
                                                                   GTCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGC                            >  SRR4302541.1114950/1‑94 (MQ=60)
                                                                    TCCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCA                           >  SRR4302541.2625180/1‑94 (MQ=60)
                                                                     CCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAG                          >  SRR4302541.1275489/1‑94 (MQ=60)
                                                                     CCACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAG                          <  SRR4302541.3906641/94‑1 (MQ=60)
                                                                      CACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGTCAGA                         >  SRR4302541.7093699/1‑94 (MQ=60)
                                                                      CACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGA                         >  SRR4302541.5092476/1‑94 (MQ=60)
                                                                      CACAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGA                         <  SRR4302541.7402021/94‑1 (MQ=60)
                                                                        CAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAG                       >  SRR4302541.8482906/1‑94 (MQ=60)
                                                                        CAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAG                       <  SRR4302541.1892500/94‑1 (MQ=60)
                                                                        CAGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAG                       >  SRR4302541.2747545/1‑94 (MQ=60)
                                                                         AGCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGGTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGA                      >  SRR4302541.1929248/1‑94 (MQ=60)
                                                                          GCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAA                     <  SRR4302541.6180718/94‑1 (MQ=60)
                                                                          GCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAA                     <  SRR4302541.1479474/94‑1 (MQ=60)
                                                                          GCCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAA                     <  SRR4302541.3721557/94‑1 (MQ=60)
                                                                           CCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAG                    <  SRR4302541.6901307/94‑1 (MQ=60)
                                                                           CCAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGATTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAG                    >  SRR4302541.8834720/1‑94 (MQ=60)
                                                                            CAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGC                   <  SRR4302541.8899959/94‑1 (MQ=60)
                                                                            CAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGC                   >  SRR4302541.8731864/1‑94 (MQ=60)
                                                                            CAGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGC                   >  SRR4302541.7271516/1‑94 (MQ=60)
                                                                             AGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCA                  >  SRR4302541.8809944/1‑94 (MQ=60)
                                                                             AGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCA                  <  SRR4302541.4427040/94‑1 (MQ=60)
                                                                             AGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCA                  >  SRR4302541.8744250/1‑94 (MQ=60)
                                                                             AGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCA                  >  SRR4302541.548805/1‑94 (MQ=60)
                                                                             AGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCA                  >  SRR4302541.8809945/1‑94 (MQ=60)
                                                                             AGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCA                  >  SRR4302541.3776422/1‑94 (MQ=60)
                                                                             AGGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCA                  >  SRR4302541.3554956/1‑94 (MQ=60)
                                                                              GGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAG                 <  SRR4302541.83476/94‑1 (MQ=60)
                                                                              GGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAG                 <  SRR4302541.5033703/94‑1 (MQ=60)
                                                                              GGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAG                 <  SRR4302541.5033702/94‑1 (MQ=60)
                                                                              GGAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAG                 <  SRR4302541.6494134/94‑1 (MQ=60)
                                                                               GAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGA                >  SRR4302541.7291114/1‑94 (MQ=60)
                                                                               GAAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGA                <  SRR4302541.2049465/94‑1 (MQ=60)
                                                                                AAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGAC               >  SRR4302541.1170205/1‑94 (MQ=60)
                                                                                AAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGAC               >  SRR4302541.7288021/1‑94 (MQ=60)
                                                                                AAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGAC               >  SRR4302541.8808990/1‑94 (MQ=60)
                                                                                AAATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGAC               <  SRR4302541.6094715/94‑1 (MQ=60)
                                                                                 AATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACC              <  SRR4302541.5460055/94‑1 (MQ=60)
                                                                                  ATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCA             >  SRR4302541.5559609/1‑94 (MQ=60)
                                                                                  ATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCA             >  SRR4302541.4541298/1‑94 (MQ=60)
                                                                                  ATCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGAAGACCA             >  SRR4302541.5524182/1‑94 (MQ=60)
                                                                                   TCTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCActcttt            <  SRR4302541.7958281/94‑7 (MQ=60)
                                                                                    CTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACC           >  SRR4302541.7855196/1‑94 (MQ=60)
                                                                                    CTTCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACC           <  SRR4302541.768250/94‑1 (MQ=60)
                                                                                      TCACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAA         <  SRR4302541.1703293/94‑1 (MQ=60)
                                                                                       CACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAG        <  SRR4302541.8204665/94‑1 (MQ=60)
                                                                                       CACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAG        >  SRR4302541.3726926/1‑94 (MQ=60)
                                                                                       CACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAG        <  SRR4302541.428117/94‑1 (MQ=60)
                                                                                       CACAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAG        >  SRR4302541.1694318/1‑94 (MQ=60)
                                                                                         CAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAA      <  SRR4302541.7354747/94‑1 (MQ=60)
                                                                                         CAGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAA      <  SRR4302541.6397434/94‑1 (MQ=60)
                                                                                          AGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAAT     <  SRR4302541.8912955/94‑1 (MQ=60)
                                                                                          AGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAAT     <  SRR4302541.8186759/94‑1 (MQ=60)
                                                                                          AGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAAT     >  SRR4302541.6324036/1‑94 (MQ=60)
                                                                                          AGGCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAAT     <  SRR4302541.5061167/94‑1 (MQ=60)
                                                                                          AGGCCCAGGAGTTGACCGGTTTTTTCTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAAT     <  SRR4302541.3523584/94‑1 (MQ=60)
                                                                                            GCCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAA   <  SRR4302541.4525893/94‑1 (MQ=60)
                                                                                             CCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAAC  <  SRR4302541.8074712/94‑1 (MQ=60)
                                                                                             CCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAAC  >  SRR4302541.3277379/1‑94 (MQ=60)
                                                                                             |                                                                                             
CATCTTCTGGTTTATCTTTGTTGTTCAGCGCTTCAGCAAAACCAACTGCAGTTGGCTGGAAGGATTCGTCCACAGCCAGGAAATCTTCACAGGTCCAGGAGTTGACCGGTTTTTTGTTATCAGCTGCTTTTTGCGCATCCGCTGCATTGCTCACAACTGGCAGAAGAAGCAGACCACCAAGAATAAC  >  NC_000913/3654166‑3654352

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑