Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,758,509	T→C	D123G (GAC→GGC)	selA ←	selenocysteine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,758,509	0	T	C	99.5%	1264.8 / ‑3.5	375	D123G (GAC→GGC)	selA	selenocysteine synthase
Reads supporting (aligned to +/- strand): ref base T (1/1); new base C (185/188); total (186/189)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.32e-01

Only 100 of 412 total aligned reads displayed.

TTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTG  >  NC_000913/3758419‑3758598

ttGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGccc                                                                                         <  2:4571749/93‑1 (MQ=255)

ttGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGccc                                                                                         >  1:5835855/1‑93 (MQ=255)

ttGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGccc                                                                                         >  1:525891/1‑93 (MQ=255)

ttGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGccc                                                                                         >  2:836361/1‑93 (MQ=255)

     aTTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATc                                                                                    <  2:6692775/93‑1 (MQ=255)

        gACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATa                                                                                 >  1:2797625/1‑93 (MQ=255)

         aCGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATAc                                                                                <  2:738798/93‑1 (MQ=255)

         aCGATACAGGCATCTTCCCCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCTCGATGTCCGCGGCCGGCGTCGCCCAGATCATAc                                                                                <  2:6296274/93‑1 (MQ=255)

          cGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACt                                                                               <  1:478785/93‑1 (MQ=255)

            aTACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCg                                                                             >  1:5519882/1‑93 (MQ=255)

             tACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGa                                                                            >  2:711316/1‑93 (MQ=255)

              aCAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAg                                                                           >  2:20436/1‑93 (MQ=255)

              aCAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAg                                                                           >  2:3417655/1‑93 (MQ=255)

                aGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAggg                                                                         <  2:5762573/93‑1 (MQ=255)

                aGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAggg                                                                         <  1:401144/93‑1 (MQ=255)

                 ggCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAgggc                                                                        >  1:1945050/1‑92 (MQ=255)

                 ggCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGt                                                                        <  2:8622388/93‑1 (MQ=255)

                 ggCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGt                                                                        >  1:7587912/1‑93 (MQ=255)

                    aTCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGTTCATACGCGAGGGTCAc                                                                     >  1:7817417/1‑93 (MQ=255)

                     tCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGTCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTAGCCCAGATCATACTCGAGGGTCACt                                                                    >  2:5119574/1‑93 (MQ=255)

                     tCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACt                                                                    >  1:8058259/1‑93 (MQ=255)

                       ttCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTgg                                                                  <  1:1663472/93‑1 (MQ=255)

                        tCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCa                                                                                   >  2:1463169/1‑75 (MQ=255)

                         ccGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCg                                                                >  1:4158148/1‑93 (MQ=255)

                          cGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGa                                                               >  1:6376257/1‑93 (MQ=255)

                           gCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGaa                                                              <  1:6464096/93‑1 (MQ=255)

                             ccccGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACg                                                            >  1:2290433/1‑93 (MQ=255)

                             ccccGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACg                                                            >  2:3195971/1‑93 (MQ=255)

                             ccccGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACg                                                            <  2:2967421/93‑1 (MQ=255)

                               ccGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCa                                                          >  1:3651440/1‑93 (MQ=255)

                                cGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCAt                                                         <  2:7913893/93‑1 (MQ=255)

                                cGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCAt                                                         >  2:2434179/1‑93 (MQ=255)

                                  tAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAg                                                       >  1:2054137/1‑93 (MQ=255)

                                  tAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAg                                                       <  2:384531/93‑1 (MQ=255)

                                    agccGGCACAGCAGCCGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGcc                                                     <  2:1403014/90‑1 (MQ=255)

                                    aTACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCTAACGCATAGcc                                                     <  2:8686924/93‑1 (MQ=255)

                                    aTACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGcc                                                     <  1:294501/93‑1 (MQ=255)

                                     tACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCt                                                    >  2:5289797/1‑93 (MQ=255)

                                      aCGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTg                                                   <  1:1612341/93‑1 (MQ=255)

                                      aCGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTg                                                   >  1:449177/1‑93 (MQ=255)

                                       cGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTgc                                                  >  2:6798818/1‑93 (MQ=255)

                                        ggCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTgcg                                                 >  1:3309879/1‑93 (MQ=255)

                                         gCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTgcgc                                                <  2:2106772/93‑1 (MQ=255)

                                         gCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTgcgc                                                <  1:3760853/93‑1 (MQ=255)

                                           acaGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCaa                                              >  2:5197501/1‑93 (MQ=255)

                                           acaGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCaa                                              <  2:784942/93‑1 (MQ=255)

                                           acaGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCaa                                              >  1:7729717/1‑93 (MQ=255)

                                           acaGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCaa                                              >  2:6379347/1‑93 (MQ=255)

                                           acaGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCaa                                              >  1:5445597/1‑93 (MQ=255)

                                            cagcagCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAAc                                             <  1:6573374/93‑1 (MQ=255)

                                             agcagcTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACg                                            <  1:7879841/93‑1 (MQ=255)

                                              gcagcTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACgg                                           >  2:4236875/1‑93 (MQ=255)

                                              gcagcTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACgg                                           >  1:8544760/1‑93 (MQ=255)

                                              gcagcTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGAGTCACTAGCGCACGCATAGCCCGCGCAACgg                                           >  1:5140872/1‑93 (MQ=255)

                                               cagcTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGc                                          <  1:428055/93‑1 (MQ=255)

                                               cagcTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGc                                          >  1:2521404/1‑93 (MQ=255)

                                                agcTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCt                                         >  1:8639592/1‑93 (MQ=255)

                                                agcTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCt                                         >  2:7068525/1‑93 (MQ=255)

                                                 gcTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCtt                                        <  2:447117/93‑1 (MQ=255)

                                                    gcgcCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCa                                     <  1:670060/93‑1 (MQ=255)

                                                     cgcCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCAc                                    <  2:7623906/93‑1 (MQ=255)

                                                     cgcCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCAc                                    >  2:2590682/1‑93 (MQ=255)

                                                      gcCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCAcc                                   <  1:4562858/93‑1 (MQ=255)

                                                       ccAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACcg                                  >  1:3460596/1‑93 (MQ=255)

                                                        cAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACcgc                                 <  1:6885920/93‑1 (MQ=255)

                                                        cAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACcgc                                 >  2:5376677/1‑93 (MQ=255)

                                                         cgcgcCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACcgcg                                <  1:5310652/92‑1 (MQ=255)

                                                          gcgcCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCgg                               <  2:5850787/93‑1 (MQ=255)

                                                            gcCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCt                             >  1:5617713/1‑93 (MQ=255)

                                                            agccGATCCCGCTGTCCGCGTCCGGCGTTGCCCAGAGCAGACTCGATGGTCACTTGCGATAGCATGGCCTGCGCAACGGCTTCCACCGCGGCt                             <  2:1162558/91‑1 (MQ=255)

                                                             cccGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCtt                            >  2:3328993/1‑93 (MQ=255)

                                                              ccGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTc                           <  2:6022341/93‑1 (MQ=255)

                                                               cGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTcc                          <  1:7189907/93‑1 (MQ=255)

                                                               cGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTcc                          >  2:821678/1‑93 (MQ=255)

                                                                 aTCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGc                        <  1:5908211/93‑1 (MQ=255)

                                                                 aTCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGc                        >  1:1008225/1‑93 (MQ=255)

                                                                    gcgATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCtt                     >  1:1732564/1‑92 (MQ=255)

                                                                    gcgATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTg                     <  2:3648578/93‑1 (MQ=255)

                                                                      gATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTa                   <  1:5062257/93‑1 (MQ=255)

                                                                      gATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTa                   >  2:472237/1‑93 (MQ=255)

                                                                       aTGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTaa                  <  1:8272310/93‑1 (MQ=255)

                                                                         gTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAg                <  1:7999312/93‑1 (MQ=255)

                                                                         gTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAg                >  2:4942038/1‑93 (MQ=255)

                                                                          tCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGc               >  1:8797944/1‑93 (MQ=255)

                                                                          tCCGCGTCCGCCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGc               <  2:1235580/93‑1 (MQ=255)

                                                                          gccGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGc               <  1:3926527/92‑1 (MQ=255)

                                                                            cgcgTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTc             <  1:6149222/93‑1 (MQ=255)

                                                                             gcgTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTcc            >  2:2285577/1‑92 (MQ=255)

                                                                               gTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGcc          <  1:4399528/93‑1 (MQ=255)

                                                                                gccGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGccc         <  2:1613798/92‑1 (MQ=255)

                                                                                 ccGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCa        <  2:6193019/93‑1 (MQ=255)

                                                                                 ccGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCa        >  1:3029875/1‑93 (MQ=255)

                                                                                  cGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCaa       >  2:2759899/1‑93 (MQ=255)

                                                                                  cGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCaa       <  2:1911991/93‑1 (MQ=255)

                                                                                  cGGCGTCGCCCAGATCATACTCGAGGGTCACTGACGAACGCATAGCCTGCGCAACGGCTTCCACCGCTGCTTCCGCCTGTAAAGCTCGCCCac       >  1:6271233/1‑92 (MQ=255)

                                                                                    gCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAgg     >  2:391491/1‑93 (MQ=255)

                                                                                    gCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAgg     <  1:2244173/93‑1 (MQ=255)

                                                                                      gtcgccCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGtt   <  2:1754573/93‑1 (MQ=255)

                                                                                       tcgccCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGTGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTg  >  1:1251900/1‑93 (MQ=255)

                                                                                       tcgccCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTg  <  1:8372743/93‑1 (MQ=255)

TTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTG  >  NC_000913/3758419‑3758598

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 19 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 235 total aligned reads displayed.

GCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTGGTAT  >  NC_000913/3758416‑3758602

GCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGC                                                                                               >  SRR4302541.4807376/1‑94 (MQ=60)

GCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGC                                                                                               >  SRR4302541.8644850/1‑94 (MQ=60)

 CATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCC                                                                                              <  SRR4302541.561025/94‑1 (MQ=60)

 CATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCC                                                                                              >  SRR4302541.2592302/1‑94 (MQ=60)

  ATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCC                                                                                             >  SRR4302541.530812/1‑94 (MQ=60)

  ATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCC                                                                                             >  SRR4302541.5895195/1‑94 (MQ=60)

  ATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCC                                                                                             >  SRR4302541.6185261/1‑94 (MQ=60)

    TGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAG                                                                                           <  SRR4302541.2718477/94‑1 (MQ=60)

       TATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATC                                                                                        <  SRR4302541.4116119/94‑1 (MQ=60)

        ATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCA                                                                                       <  SRR4302541.4451741/94‑1 (MQ=60)

         cttccgatctggaacttTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCAT                                                                                      <  SRR4302541.1477497/77‑1 (MQ=60)

           GACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATAC                                                                                    >  SRR4302541.5797618/1‑94 (MQ=60)

           GACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATAC                                                                                    >  SRR4302541.4575665/1‑94 (MQ=60)

           GACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATAC                                                                                    <  SRR4302541.7202670/94‑1 (MQ=60)

             CGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTC                                                                                  <  SRR4302541.483325/94‑1 (MQ=60)

             CGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCAGGCGTCGCCCAGCTCATACTC                                                                                  >  SRR4302541.5180053/1‑94 (MQ=60)

              GATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCG                                                                                 >  SRR4302541.1365131/1‑94 (MQ=60)

              GATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCG                                                                                 >  SRR4302541.2636912/1‑94 (MQ=60)

              GATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCG                                                                                 >  SRR4302541.5576386/1‑94 (MQ=60)

              GATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCG                                                                                 >  SRR4302541.7383579/1‑94 (MQ=60)

               ATACAGGCATCTTCCGCCCCCGTAATCCGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGA                                                                                <  SRR4302541.6424681/94‑1 (MQ=60)

               ATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGA                                                                                <  SRR4302541.1736157/94‑1 (MQ=60)

               ATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGA                                                                                >  SRR4302541.4044241/1‑94 (MQ=60)

               ATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATAATACTCGA                                                                                >  SRR4302541.5935800/1‑94 (MQ=60)

                TACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAG                                                                               >  SRR4302541.5017153/1‑94 (MQ=60)

                TACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAG                                                                               >  SRR4302541.5841670/1‑94 (MQ=60)

                TACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAG                                                                               <  SRR4302541.2036321/94‑1 (MQ=60)

                TACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAG                                                                               <  SRR4302541.2294525/94‑1 (MQ=60)

                 ACAGGCATCTTCCGTCCCTGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGG                                                                              <  SRR4302541.7856502/94‑1 (MQ=60)

                 ACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATCTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGG                                                                              >  SRR4302541.6611633/1‑94 (MQ=60)

                  CAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGG                                                                             >  SRR4302541.5344864/1‑94 (MQ=60)

                   AGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGT                                                                            <  SRR4302541.6927913/94‑1 (MQ=60)

                   AGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGT                                                                            >  SRR4302541.7668980/1‑94 (MQ=60)

                   AGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGT                                                                            <  SRR4302541.404858/94‑1 (MQ=60)

                   AGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGC                                                                            >  SRR4302541.1963785/1‑94 (MQ=60)

                   AGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGC                                                                            >  SRR4302541.3063354/1‑94 (MQ=60)

                    GGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTC                                                                           <  SRR4302541.2853543/94‑1 (MQ=60)

                    GGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTC                                                                           >  SRR4302541.7708958/1‑94 (MQ=60)

                     GCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCA                                                                          <  SRR4302541.2319048/94‑1 (MQ=60)

                      CATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGTTCATACGCGAGGGTCAC                                                                         >  SRR4302541.7902099/1‑94 (MQ=60)

                      CATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCAC                                                                         >  SRR4302541.483530/1‑94 (MQ=60)

                       ATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACT                                                                        >  SRR4302541.8146807/1‑94 (MQ=60)

                       ATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACT                                                                        >  SRR4302541.200286/1‑94 (MQ=60)

                        TCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTG                                                                       <  SRR4302541.1647505/94‑1 (MQ=60)

                        TCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTG                                                                       <  SRR4302541.8399559/94‑1 (MQ=60)

                         CTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGG                                                                      >  SRR4302541.4241884/1‑94 (MQ=60)

                          TTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGC                                                                     <  SRR4302541.1679886/94‑1 (MQ=60)

                          TTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGC                                                                     >  SRR4302541.1007010/1‑94 (MQ=60)

                          TTCCGCCCCCGTAATACGGCACAGCAGCTACGCCAGCGCCCGATCGCGATGTCAGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGC                                                                     >  SRR4302541.6142339/1‑94 (MQ=60)

                           TCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCG                                                                    >  SRR4302541.4199295/1‑94 (MQ=60)

                            CCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGA                                                                   <  SRR4302541.5426125/94‑1 (MQ=60)

                            CCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGA                                                                   >  SRR4302541.6441408/1‑94 (MQ=60)

                            CCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTAGAGGGTCACTGGCGA                                                                   >  SRR4302541.3922483/1‑94 (MQ=60)

                             CGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAA                                                                  >  SRR4302541.4608329/1‑94 (MQ=60)

                              GCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAAC                                                                 >  SRR4302541.1689941/1‑94 (MQ=60)

                              GCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAAC                                                                 <  SRR4302541.6529970/94‑1 (MQ=60)

                              CCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAAC                                                                 <  SRR4302541.3015842/94‑1 (MQ=60)

                               CCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACG                                                                >  SRR4302541.6560106/1‑94 (MQ=60)

                               CCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACG                                                                <  SRR4302541.6455/94‑1 (MQ=60)

                               CCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACG                                                                >  SRR4302541.2312613/1‑94 (MQ=60)

                               CCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACG                                                                <  SRR4302541.8859336/94‑1 (MQ=60)

                                CCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGC                                                               >  SRR4302541.646541/1‑94 (MQ=60)

                                 CCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCA                                                              >  SRR4302541.3688482/1‑94 (MQ=60)

                                  CCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCAT                                                             <  SRR4302541.1089779/94‑1 (MQ=60)

                                   CGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATA                                                            <  SRR4302541.2968908/94‑1 (MQ=60)

                                   CGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATA                                                            <  SRR4302541.8802698/94‑1 (MQ=60)

                                    GTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAG                                                           <  SRR4302541.5157039/94‑1 (MQ=60)

                                    GTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAG                                                           <  SRR4302541.827012/94‑1 (MQ=60)

                                    GTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAG                                                           <  SRR4302541.5540114/94‑1 (MQ=60)

                                    GTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAG                                                           >  SRR4302541.2073966/1‑94 (MQ=60)

                                    GTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAG                                                           <  SRR4302541.5995984/94‑1 (MQ=60)

                                    GTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAG                                                           >  SRR4302541.3838623/1‑94 (MQ=60)

                                     TAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTCGCGAACGCATAAC                                                          >  SRR4302541.6234540/1‑94 (MQ=60)

                                      AATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCC                                                         <  SRR4302541.3448667/94‑1 (MQ=60)

                                      AATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCC                                                         >  SRR4302541.5169850/1‑94 (MQ=60)

                                       ATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCT                                                        <  SRR4302541.297062/94‑1 (MQ=60)

                                        TACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTG                                                       >  SRR4302541.453455/1‑94 (MQ=60)

                                         ACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGC                                                      <  SRR4302541.1628360/94‑1 (MQ=60)

                                          CGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCG                                                     >  SRR4302541.5318138/1‑94 (MQ=60)

                                          CGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCG                                                     <  SRR4302541.2299988/94‑1 (MQ=60)

                                          CGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCG                                                     >  SRR4302541.3343115/1‑94 (MQ=60)

                                          CGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACACGAGGGTCACTGGCGAACGCATAGCCTGCG                                                     <  SRR4302541.7917667/94‑1 (MQ=60)

                                            GCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCA                                                   <  SRR4302541.6026679/94‑1 (MQ=60)

                                             CACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAA                                                  <  SRR4302541.3182689/94‑1 (MQ=60)

                                             CACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAA                                                  >  SRR4302541.5501475/1‑94 (MQ=60)

                                             CACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAA                                                  >  SRR4302541.7811951/1‑94 (MQ=60)

                                              ACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAAC                                                 <  SRR4302541.5648106/94‑1 (MQ=60)

                                              ACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAAC                                                 <  SRR4302541.2984598/94‑1 (MQ=60)

                                              ACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAAC                                                 <  SRR4302541.2169018/94‑1 (MQ=60)

                                              ACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAAC                                                 >  SRR4302541.7669509/1‑94 (MQ=60)

                                              ACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAAC                                                 <  SRR4302541.4286566/94‑1 (MQ=60)

                                               CAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACG                                                <  SRR4302541.8021387/94‑1 (MQ=60)

                                               CAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACG                                                <  SRR4302541.6640627/94‑1 (MQ=60)

                                               CAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACG                                                <  SRR4302541.6297251/94‑1 (MQ=60)

                                               CAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACG                                                <  SRR4302541.2390027/94‑1 (MQ=60)

                                               CAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACG                                                <  SRR4302541.8099962/94‑1 (MQ=60)

                                                AGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGG                                               <  SRR4302541.7966455/94‑1 (MQ=60)

                                                AGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGG                                               >  SRR4302541.1066686/1‑94 (MQ=60)

                                                AGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGG                                               >  SRR4302541.8636612/1‑94 (MQ=60)

                                                AGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGAGTCACTAGCGCACGCATAGCCCGCGCAACGG                                               >  SRR4302541.5193749/1‑94 (MQ=60)

                                                 GCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGC                                              >  SRR4302541.2546776/1‑94 (MQ=60)

                                                  CAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCT                                             <  SRR4302541.432067/94‑1 (MQ=60)

                                                  CAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCT                                             >  SRR4302541.8732141/1‑94 (MQ=60)

                                                  CAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCT                                             <  SRR4302541.432050/94‑1 (MQ=60)

                                                  CAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCT                                             <  SRR4302541.8670011/94‑1 (MQ=60)

                                                  CACCTGCGCCAGCGCCCCATCGCGATGTCCTCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCT                                             <  SRR4302541.8670012/94‑1 (MQ=60)

                                                   CGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATGCTCGAGGGTCCCTGGCGAACGCATAGCCTGCGCAACGGCTT                                            <  SRR4302541.4445772/94‑1 (MQ=60)

                                                   AGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGTGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTT                                            >  SRR4302541.3592892/1‑94 (MQ=60)

                                                   AGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTT                                            <  SRR4302541.4445771/94‑1 (MQ=60)

                                                    GCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTC                                           <  SRR4302541.7447431/94‑1 (MQ=60)

                                                    GCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTC                                           <  SRR4302541.4370634/94‑1 (MQ=60)

                                                     CTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCC                                          <  SRR4302541.8517095/94‑1 (MQ=60)

                                                     CTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCC                                          <  SRR4302541.4696428/94‑1 (MQ=60)

                                                      CGCGCCAGCGCCCGATCGCGAGGTCCGCGACCGCCGTCGCGCAGATCATACTCGAGGGTCACTGTCGAACGCATAGCCTGCGCAACGGCTTCCA                                         <  SRR4302541.6219847/94‑1 (MQ=60)

                                                       GCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCAC                                        <  SRR4302541.5379300/94‑1 (MQ=60)

                                                       GCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCAC                                        <  SRR4302541.676381/94‑1 (MQ=60)

                                                        CGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACC                                       <  SRR4302541.5694118/94‑1 (MQ=60)

                                                        CGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACC                                       >  SRR4302541.7335971/1‑94 (MQ=60)

                                                        CGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACC                                       <  SRR4302541.1641454/94‑1 (MQ=60)

                                                         GCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAGCGCATAGCCTGCGCAACGGCTTCCACCG                                      <  SRR4302541.1166816/94‑1 (MQ=60)

                                                         GCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCG                                      >  SRR4302541.3495179/1‑94 (MQ=60)

                                                         GCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCG                                      <  SRR4302541.6215485/94‑1 (MQ=60)

                                                         GCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCG                                      <  SRR4302541.4609054/94‑1 (MQ=60)

                                                         GCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCC                                      >  SRR4302541.8813955/1‑94 (MQ=60)

                                                          CCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGC                                     >  SRR4302541.3374353/1‑94 (MQ=60)

                                                          CCAGCGCCCGATCGAGATGTCCGCGTCCGGCGTCGCACAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGC                                     <  SRR4302541.7887462/94‑1 (MQ=60)

                                                           CAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCG                                    <  SRR4302541.8109952/94‑1 (MQ=60)

                                                           CAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCG                                    >  SRR4302541.5243857/1‑94 (MQ=60)

                                                           CAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCG                                    <  SRR4302541.6959418/94‑1 (MQ=60)

                                                            CGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGG                                   <  SRR4302541.5365316/94‑1 (MQ=60)

                                                              CGCCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCT                                 >  SRR4302541.5675204/1‑94 (MQ=60)

                                                                CCCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTC                               >  SRR4302541.2224729/1‑94 (MQ=60)

                                                                 CCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCC                              >  SRR4302541.540392/1‑94 (MQ=60)

                                                                 CCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCC                              <  SRR4302541.8072866/94‑1 (MQ=60)

                                                                 CCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCC                              <  SRR4302541.1422882/94‑1 (MQ=60)

                                                                 CCGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCC                              >  SRR4302541.8515481/1‑94 (MQ=60)

                                                                  CGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCG                             <  SRR4302541.7266598/94‑1 (MQ=60)

                                                                  CGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCG                             <  SRR4302541.3708043/94‑1 (MQ=60)

                                                                  CGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCG                             >  SRR4302541.5259912/1‑94 (MQ=60)

                                                                  CGATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCG                             >  SRR4302541.8487961/1‑94 (MQ=60)

                                                                   GATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGC                            >  SRR4302541.7858255/1‑94 (MQ=60)

                                                                   GATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGC                            >  SRR4302541.1017571/1‑94 (MQ=60)

                                                                   GATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGC                            >  SRR4302541.4770521/1‑94 (MQ=60)

                                                                   GATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGC                            <  SRR4302541.3893312/94‑1 (MQ=60)

                                                                    ATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCC                           <  SRR4302541.5968352/94‑1 (MQ=60)

                                                                    ATCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCGGCGCAACGGCTTCCACCGCGGCTTCCGCC                           <  SRR4302541.5968349/94‑1 (MQ=60)

                                                                     TCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCT                          <  SRR4302541.4010336/94‑1 (MQ=60)

                                                                     TCGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCT                          <  SRR4302541.7699469/94‑1 (MQ=60)

                                                                      CGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTT                         >  SRR4302541.1749483/1‑94 (MQ=60)

                                                                      CGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTG                         <  SRR4302541.6699080/94‑1 (MQ=60)

                                                                      CGCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTG                         >  SRR4302541.8023020/1‑94 (MQ=60)

                                                                       GCGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGT                        <  SRR4302541.7157725/94‑1 (MQ=60)

                                                                        CGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTA                       >  SRR4302541.7912555/1‑94 (MQ=60)

                                                                        CGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTA                       >  SRR4302541.558963/1‑94 (MQ=60)

                                                                        CGATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTA                       >  SRR4302541.1691775/1‑94 (MQ=60)

                                                                         GATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAA                      <  SRR4302541.6772854/94‑1 (MQ=60)

                                                                         GATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAA                      >  SRR4302541.284399/1‑94 (MQ=60)

                                                                         GATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAA                      <  SRR4302541.5114313/94‑1 (MQ=60)

                                                                          ATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAA                     <  SRR4302541.8362328/94‑1 (MQ=60)

                                                                          ATGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAA                     >  SRR4302541.6373612/1‑94 (MQ=60)

                                                                           TGTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAG                    <  SRR4302541.1864943/94‑1 (MQ=60)

                                                                            GTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGC                   <  SRR4302541.8087339/94‑1 (MQ=60)

                                                                            GTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGC                   >  SRR4302541.6617098/1‑94 (MQ=60)

                                                                            GTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGC                   >  SRR4302541.8891906/1‑94 (MQ=60)

                                                                            GTCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGC                   >  SRR4302541.2776741/1‑94 (MQ=60)

                                                                             GCCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCT                  <  SRR4302541.3965663/94‑1 (MQ=60)

                                                                              CCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTC                 >  SRR4302541.8176534/1‑94 (MQ=60)

                                                                              CCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTC                 >  SRR4302541.4079477/1‑94 (MQ=60)

                                                                              CCGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTC                 >  SRR4302541.4910548/1‑94 (MQ=60)

                                                                               CGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCG                <  SRR4302541.234743/94‑1 (MQ=60)

                                                                               CGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCG                <  SRR4302541.2246020/94‑1 (MQ=60)

                                                                               CGCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCG                <  SRR4302541.8372794/94‑1 (MQ=60)

                                                                                GCGTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGC               <  SRR4302541.3271861/94‑1 (MQ=60)

                                                                                  GTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCC             >  SRR4302541.3872365/1‑94 (MQ=60)

                                                                                  GTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCC             <  SRR4302541.4443534/94‑1 (MQ=60)

                                                                                  GTCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCC             >  SRR4302541.6744386/1‑94 (MQ=60)

                                                                                  GTCCGGCCTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCC             <  SRR4302541.1764419/94‑1 (MQ=60)

                                                                                   TCCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCA            >  SRR4302541.3060461/1‑94 (MQ=60)

                                                                                    CCGGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAA           <  SRR4302541.3742636/94‑1 (MQ=60)

                                                                                    CCGGCGTCGCCCAGATCATACTCGAGGGTCACTGACGAACGCATAGCCTGCGCAACGGCTTCCACCGCTGCTTCCGCCTGTAAAGCTCGCCCAC           >  SRR4302541.6335263/1‑94 (MQ=60)

                                                                                      GGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGG         >  SRR4302541.8116423/1‑94 (MQ=60)

                                                                                      GGCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGG         >  SRR4302541.5511939/1‑94 (MQ=60)

                                                                                       GCGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGT        <  SRR4302541.2265892/94‑1 (MQ=60)

                                                                                        CGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTTCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAcgggt       >  SRR4302541.3757110/1‑89 (MQ=60)

                                                                                        CGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAACGCTCGCCCAAGGTT       >  SRR4302541.6783539/1‑94 (MQ=60)

                                                                                        CGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTT       >  SRR4302541.3527142/1‑94 (MQ=60)

                                                                                        CGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTT       <  SRR4302541.8446968/94‑1 (MQ=60)

                                                                                        CGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTT       >  SRR4302541.4120471/1‑94 (MQ=60)

                                                                                        CGTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTT       <  SRR4302541.185040/94‑1 (MQ=60)

                                                                                         GTCGCCGAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTG      >  SRR4302541.5304388/1‑94 (MQ=60)

                                                                                         GTCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGTGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTG      >  SRR4302541.1264315/1‑94 (MQ=60)

                                                                                          TCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTGG     <  SRR4302541.8463477/94‑1 (MQ=60)

                                                                                          TCGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTGG     >  SRR4302541.4404835/1‑94 (MQ=60)

                                                                                           CGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTGGT    >  SRR4302541.6280491/1‑94 (MQ=60)

                                                                                           CGCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTGGT    <  SRR4302541.4661262/94‑1 (MQ=60)

                                                                                            GCCCAGATCATGCTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTGGTA   <  SRR4302541.2249699/94‑1 (MQ=60)

                                                                                            GCCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTGGTA   >  SRR4302541.3087632/1‑94 (MQ=60)

                                                                                             CCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTGGTAT  <  SRR4302541.4543910/94‑1 (MQ=60)

                                                                                             CCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTGGTAT  <  SRR4302541.3514581/94‑1 (MQ=60)

                                                                                             CCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTACGCCTGTAAAGCTCGCCCAAGGTTGGTAT  >  SRR4302541.3760246/1‑94 (MQ=60)

GCATTGTTATTGACGATACAGGCATCTTCCGCCCCCGTAATACGGCACAGCAGCTGCGCCAGCGCCCGATCGCGATGTCCGCGTCCGGCGTCGTCCAGATCATACTCGAGGGTCACTGGCGAACGCATAGCCTGCGCAACGGCTTCCACCGCGGCTTCCGCCTGTAAAGCTCGCCCAAGGTTGGTAT  >  NC_000913/3758416‑3758602

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`