Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,764,283 T→C V107A (GTT→GCT)  yibA → hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,764,2830TC99.3% 1345.4 / ‑4.0 407V107A (GTT→GCT) yibAhypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (191/213);  total (193/214)
Fisher's exact test for biased strand distribution p-value = 6.06e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 421 total aligned reads displayed.

TACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTG  >  NC_000913/3764193‑3764372
                                                                                          |                                                                                         
taCTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTc                                                                                         <  2:2479717/93‑1 (MQ=255)
taCTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTc                                                                                         <  1:6154634/93‑1 (MQ=255)
 aCTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCg                                                                                        >  2:7780917/1‑93 (MQ=255)
      ggCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTa                                                                                   <  1:1634579/93‑1 (MQ=255)
       gCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTAc                                                                                  <  1:6537875/93‑1 (MQ=37)
        cAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACg                                                                                 >  1:8529777/1‑93 (MQ=255)
         aaaTAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACgg                                                                                <  1:7026805/93‑1 (MQ=255)
         aaaTAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACgg                                                                                >  2:6756634/1‑93 (MQ=255)
          aaTAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGc                                                                               >  2:4901447/1‑93 (MQ=37)
               aaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCg                                                                          <  1:442336/93‑1 (MQ=21)
               aaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCg                                                                          <  2:6042291/93‑1 (MQ=18)
               aaaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCg                                                                          <  2:7371678/93‑1 (MQ=21)
                aaTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGa                                                                         <  1:980053/93‑1 (MQ=18)
                 aTTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAg                                                                        >  1:4326605/1‑93 (MQ=18)
                   ttGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTc                                                                      <  1:8268824/93‑1 (MQ=21)
                     gCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCaa                                                                    <  1:1921429/93‑1 (MQ=18)
                      cAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAAc                                                                   <  2:5372326/93‑1 (MQ=21)
                      cAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAAc                                                                   <  2:4249353/93‑1 (MQ=18)
                       aaaaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACg                                                                  >  1:1357609/1‑93 (MQ=18)
                        aaaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACgg                                                                 >  1:2975169/1‑93 (MQ=18)
                         aaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGc                                                                <  2:2415252/93‑1 (MQ=21)
                         aaaaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGc                                                                >  2:1259090/1‑93 (MQ=21)
                           aaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTc                                                              >  1:4878770/1‑93 (MQ=25)
                           aaTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTc                                                              <  1:1845126/93‑1 (MQ=25)
                            aTGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCa                                                             >  2:7834827/1‑93 (MQ=37)
                             tGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAg                                                            <  2:5109737/93‑1 (MQ=255)
                             tGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAg                                                            >  1:1447045/1‑93 (MQ=255)
                              gCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGc                                                           <  1:2092442/93‑1 (MQ=255)
                               cGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCg                                                          >  1:1110058/1‑93 (MQ=255)
                                gAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGa                                                         <  2:2865718/93‑1 (MQ=255)
                                 aaGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGAt                                                        >  1:3520877/1‑93 (MQ=255)
                                 aaGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTGGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGAt                                                        <  2:3757889/93‑1 (MQ=255)
                                   gATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGc                                                      <  2:8790135/93‑1 (MQ=255)
                                     tAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCaa                                                    <  1:6415060/93‑1 (MQ=255)
                                     tAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCaa                                                    >  1:4778429/1‑93 (MQ=255)
                                     tAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCaa                                                    <  1:8734518/93‑1 (MQ=255)
                                     tAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAACTACGGCAATCGAGTCAACGGCTCAGCGATGCaa                                                    >  1:5363708/1‑93 (MQ=255)
                                     ataTGTTGGTAATATTGTGAACAATAGGGCATTGAACGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCTAGTCAACTGCTCAGCGATGCaa                                                    <  1:4537134/91‑1 (MQ=17)
                                      aaTGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAg                                                   >  1:1232170/1‑93 (MQ=255)
                                      aaTGTTTTTAATATTTTGAACAATAT‑GCATTGCATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATTGAGTCAACGCCTCAGCGAGGCAAGa                                                  >  2:4516650/1‑93 (MQ=14)
                                       aTGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGa                                                  <  2:4824213/93‑1 (MQ=255)
                                        tGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaa                                                 >  2:3027301/1‑93 (MQ=255)
                                        tGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaa                                                 >  2:7518433/1‑93 (MQ=255)
                                        tGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaa                                                 >  2:6686936/1‑93 (MQ=255)
                                         gTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaaa                                                >  2:6618237/1‑93 (MQ=255)
                                          tttttAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaaaa                                               >  2:8080598/1‑93 (MQ=255)
                                           ttttAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGaaaaa                                              >  1:6625819/1‑93 (MQ=255)
                                            tttAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAAc                                             >  2:3304762/1‑93 (MQ=255)
                                             ttAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAAcc                                            >  2:8573884/1‑93 (MQ=255)
                                                atatTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCaa                                         >  2:2583452/1‑93 (MQ=255)
                                                 tatTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAt                                        <  1:5020647/93‑1 (MQ=255)
                                                 tatTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAt                                        <  1:1769044/93‑1 (MQ=255)
                                                 tatTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAt                                        >  1:2807540/1‑93 (MQ=255)
                                                  atTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAtt                                       <  1:2202834/93‑1 (MQ=255)
                                                   ttttGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAttt                                      <  1:413766/93‑1 (MQ=255)
                                                    tttGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCTACGGCTCAGCGATGCAAGAAAAACCCAATTTa                                     >  2:4591953/1‑93 (MQ=255)
                                                    tttGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTa                                     <  2:4756527/93‑1 (MQ=255)
                                                       gAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTc                                  >  2:934029/1‑93 (MQ=255)
                                                        aaCAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCa                                 <  1:3530819/93‑1 (MQ=255)
                                                         aCAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCAc                                >  1:4072845/1‑93 (MQ=255)
                                                         aCAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCAc                                >  2:6979888/1‑93 (MQ=255)
                                                          cAATATGGCATTGAATAATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCAcc                               <  1:1559965/93‑1 (MQ=255)
                                                           aaTATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCt                              <  2:2715336/93‑1 (MQ=255)
                                                           aaTATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCt                              >  2:3887014/1‑93 (MQ=255)
                                                             tttGGTATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCATCGGCTCAGCGTTGCAAGAAATACCCAATTTATTCACCTaa                            >  1:7805918/3‑93 (MQ=21)
                                                             tatGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaa                            >  1:2493434/1‑93 (MQ=255)
                                                             tatGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaa                            >  2:1855486/1‑93 (MQ=255)
                                                             aatGGCATTGAATGATAAGAGCGCGTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaa                            <  2:6461904/92‑1 (MQ=255)
                                                               tGGCATTGATTGATAAGAGCCCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACACAATTTATTCCCCTaaaa                          <  2:4122780/93‑1 (MQ=25)
                                                               tGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTaaaa                          <  2:5803609/93‑1 (MQ=255)
                                                                 gCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAta                        <  1:5234334/93‑1 (MQ=255)
                                                                 gCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAta                        <  2:3521090/93‑1 (MQ=255)
                                                                  cATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAtag                       >  2:7658942/1‑93 (MQ=255)
                                                                  cATTGAATAATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAtag                       <  1:3208246/93‑1 (MQ=255)
                                                                    ttGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAtagta                     >  1:8084610/1‑93 (MQ=255)
                                                                     tGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAtagtag                    >  1:7589972/1‑93 (MQ=255)
                                                                     tGAATGATAAGACGGCTTGCGCTCGATCTCCGGCAATCGAGTCACCGACTCAACCATGCAAGACAATCCCAATTTACTTACCTAAAAtagtgc                    >  2:6114855/1‑91 (MQ=16)
                                                                        aTGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAAc                 >  1:3937251/1‑93 (MQ=255)
                                                                        aTGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTACAAc                 >  2:3126163/1‑93 (MQ=255)
                                                                          gATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACaa               <  1:3855132/93‑1 (MQ=255)
                                                                            tAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAAtc             <  2:5097888/93‑1 (MQ=255)
                                                                            tAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAAtc             <  1:604386/93‑1 (MQ=255)
                                                                             aaGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAAtct            >  1:7209262/1‑93 (MQ=255)
                                                                              agagCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAAtctc           <  1:3052401/93‑1 (MQ=255)
                                                                               gagCGCTTGCGCTCGAGCTACGGCAATCGAGGCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCa          >  2:5189534/1‑93 (MQ=255)
                                                                                agCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCaa         >  2:1607909/1‑93 (MQ=255)
                                                                                 gcgcTTGCGCTCGAGGTACGGCAATCGAGTCAACGGCTCAGCGATGCAAAAAAAACCCAATTTATTCCCCTAAAATAGTAGAACAATCTCaaa        <  2:5694199/93‑1 (MQ=38)
                                                                                 gcgcTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCaaa        <  1:3684170/93‑1 (MQ=255)
                                                                                  cgcTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAAt       <  1:6923225/93‑1 (MQ=255)
                                                                                  cgcTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAAt       >  1:7532250/1‑93 (MQ=255)
                                                                                    cTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTa     <  2:222295/93‑1 (MQ=255)
                                                                                    cTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTa     >  1:5971289/1‑93 (MQ=255)
                                                                                    cTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTa     >  1:262553/1‑93 (MQ=255)
                                                                                    cTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTa     >  1:7700942/1‑93 (MQ=255)
                                                                                     ttGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTAc    <  1:3781630/93‑1 (MQ=255)
                                                                                     ttGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTAc    <  1:5688550/93‑1 (MQ=255)
                                                                                      tGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACt   <  2:5942632/93‑1 (MQ=255)
                                                                                      tGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACt   >  1:8666399/1‑93 (MQ=255)
                                                                                      tGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACt   <  1:7406913/93‑1 (MQ=255)
                                                                                       gcgcTCGAGCTACGGCAATCGAGTCAACGGCTTAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACta  <  1:1002652/93‑2 (MQ=255)
                                                                                          |                                                                                         
TACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTG  >  NC_000913/3764193‑3764372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 242 total aligned reads displayed.
TTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTT  >  NC_000913/3764190‑3764376
                                                                                             |                                                                                             
TTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGC                                                                                               >  SRR4302541.3429721/1‑94 (MQ=26)
 TATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCT                                                                                              >  SRR4302541.6442336/1‑94 (MQ=26)
 TATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCT                                                                                              <  SRR4302541.8088532/94‑1 (MQ=26)
  ATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTC                                                                                             >  SRR4302541.2185282/1‑94 (MQ=26)
   TACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCG                                                                                            <  SRR4302541.6217524/94‑1 (MQ=25)
   TACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCG                                                                                            >  SRR4302541.6773768/1‑94 (MQ=25)
      TCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGC                                                                                         >  SRR4302541.5384270/1‑94 (MQ=25)
      TCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGC                                                                                         <  SRR4302541.7746447/94‑1 (MQ=25)
       CGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCT                                                                                        >  SRR4302541.1788350/1‑94 (MQ=25)
         GGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTAC                                                                                      <  SRR4302541.1650749/94‑1 (MQ=25)
         GGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTAC                                                                                      <  SRR4302541.1286139/94‑1 (MQ=25)
          GCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACG                                                                                     >  SRR4302541.4059610/1‑94 (MQ=25)
          GCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACG                                                                                     <  SRR4302541.6604638/94‑1 (MQ=25)
          GCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACG                                                                                     >  SRR4302541.8621534/1‑94 (MQ=25)
          GCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACG                                                                                     <  SRR4302541.8897094/94‑1 (MQ=25)
          GCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACG                                                                                     <  SRR4302541.3709811/94‑1 (MQ=25)
           CAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGG                                                                                    >  SRR4302541.7515039/1‑94 (MQ=25)
            AAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGC                                                                                   <  SRR4302541.7101509/94‑1 (MQ=25)
            AAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGC                                                                                   <  SRR4302541.7771923/94‑1 (MQ=25)
            AAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGC                                                                                   >  SRR4302541.2527351/1‑94 (MQ=25)
             AATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCA                                                                                  <  SRR4302541.4453008/94‑1 (MQ=25)
             AATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCA                                                                                  >  SRR4302541.4299248/1‑94 (MQ=25)
              ATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAA                                                                                 >  SRR4302541.7267465/1‑94 (MQ=23)
              ATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAA                                                                                 >  SRR4302541.1316045/1‑94 (MQ=23)
               TAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAAT                                                                                <  SRR4302541.7864875/94‑1 (MQ=20)
                AAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATC                                                                               <  SRR4302541.6034090/94‑1 (MQ=15)
                AAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATC                                                                               <  SRR4302541.4161106/94‑1 (MQ=15)
                 AAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCG                                                                              <  SRR4302541.6358326/94‑1 (MQ=12)
                  AAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGA                                                                             >  SRR4302541.5268073/1‑94 (MQ=10)
                  AAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGA                                                                             <  SRR4302541.446493/94‑1 (MQ=10)
                  AAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGA                                                                             <  SRR4302541.1162907/94‑1 (MQ=10)
                  AAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGA                                                                             <  SRR4302541.815724/94‑1 (MQ=10)
                   AATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAG                                                                            >  SRR4302541.3556515/1‑94 (MQ=10)
                      TTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCA                                                                         <  SRR4302541.1868637/94‑1 (MQ=10)
                        GCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAAC                                                                       <  SRR4302541.1939928/94‑1 (MQ=10)
                         CAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAAGG                                                                      >  SRR4302541.4825710/1‑94 (MQ=9)
                         CAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACG                                                                      <  SRR4302541.7625243/94‑1 (MQ=10)
                         CAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACG                                                                      <  SRR4302541.638794/94‑1 (MQ=10)
                         CAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACG                                                                      >  SRR4302541.1371016/1‑94 (MQ=10)
                         CAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACG                                                                      <  SRR4302541.7625250/94‑1 (MQ=10)
                         CAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACG                                                                      <  SRR4302541.7354401/94‑1 (MQ=10)
                          AAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGG                                                                     >  SRR4302541.3005157/1‑94 (MQ=10)
                          AAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGG                                                                     <  SRR4302541.1572465/94‑1 (MQ=10)
                          AAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGC                                                                     >  SRR4302541.3005158/1‑94 (MQ=9)
                             AAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTC                                                                  >  SRR4302541.8296511/1‑94 (MQ=15)
                             AAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTC                                                                  >  SRR4302541.4928556/1‑94 (MQ=15)
                             AAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTC                                                                  >  SRR4302541.2886386/1‑94 (MQ=15)
                             AAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTC                                                                  <  SRR4302541.2378192/94‑1 (MQ=15)
                              AATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCA                                                                 <  SRR4302541.1862980/94‑1 (MQ=20)
                              AATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCA                                                                 <  SRR4302541.3455843/94‑1 (MQ=20)
                               ATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAG                                                                <  SRR4302541.6188295/94‑1 (MQ=23)
                               ATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAG                                                                <  SRR4302541.2097414/94‑1 (MQ=23)
                               ATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAG                                                                >  SRR4302541.1461127/1‑94 (MQ=23)
                               ATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAG                                                                >  SRR4302541.8475290/1‑94 (MQ=23)
                                TGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGC                                                               <  SRR4302541.4863013/94‑1 (MQ=25)
                                TGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGC                                                               <  SRR4302541.3920634/94‑1 (MQ=25)
                                 GCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCG                                                              >  SRR4302541.1120488/1‑94 (MQ=28)
                                 GCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCG                                                              <  SRR4302541.5670266/94‑1 (MQ=28)
                                 GCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCG                                                              <  SRR4302541.2112718/94‑1 (MQ=28)
                                   GAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGAT                                                            >  SRR4302541.3669662/1‑94 (MQ=33)
                                   GAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGAT                                                            <  SRR4302541.6806209/94‑1 (MQ=33)
                                    AAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATG                                                           <  SRR4302541.490274/94‑1 (MQ=35)
                                    AAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATG                                                           <  SRR4302541.1903248/94‑1 (MQ=35)
                                    AAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATG                                                           <  SRR4302541.3560550/94‑1 (MQ=35)
                                     AGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGA                                                          >  SRR4302541.1017348/1‑94 (MQ=35)
                                      GATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCA                                                         >  SRR4302541.2163183/1‑94 (MQ=40)
                                      GATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCA                                                         >  SRR4302541.8612755/1‑94 (MQ=40)
                                       ATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAA                                                        >  SRR4302541.5418788/1‑94 (MQ=43)
                                       ATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAA                                                        >  SRR4302541.2732047/1‑94 (MQ=43)
                                       ATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAA                                                        <  SRR4302541.1655811/94‑1 (MQ=43)
                                       ATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAACTACGGCAATCGAGTCAACGGCTCAGCGATGCAA                                                        >  SRR4302541.5418789/1‑94 (MQ=41)
                                        TAATTTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAG                                                       <  SRR4302541.7217327/94‑1 (MQ=43)
                                        TAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAG                                                       <  SRR4302541.4582983/94‑1 (MQ=45)
                                        TAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAG                                                       <  SRR4302541.8819312/94‑1 (MQ=45)
                                        TAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAG                                                       <  SRR4302541.8828026/94‑1 (MQ=45)
                                        TAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAG                                                       <  SRR4302541.938173/94‑1 (MQ=45)
                                        TAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAG                                                       >  SRR4302541.1244293/1‑94 (MQ=45)
                                         AATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGA                                                      <  SRR4302541.369939/94‑1 (MQ=48)
                                         AATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGA                                                      >  SRR4302541.7020985/1‑94 (MQ=48)
                                         AATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGAGCTCGAGCTACGGCAATCGAGCCAACGGCTCAGCGATGCAAGA                                                      >  SRR4302541.7020687/1‑94 (MQ=44)
                                          ATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAA                                                     <  SRR4302541.6114972/94‑1 (MQ=50)
                                          ATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAA                                                     <  SRR4302541.1554888/94‑1 (MQ=50)
                                           TGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAA                                                    <  SRR4302541.1824220/94‑1 (MQ=53)
                                             TTTTTAATATTTTGAACAATATGGCATTGAATTATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAA                                                  >  SRR4302541.4360231/1‑94 (MQ=55)
                                             TTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAA                                                  <  SRR4302541.6647418/94‑1 (MQ=58)
                                             TTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAA                                                  >  SRR4302541.6693741/1‑94 (MQ=58)
                                             TTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAA                                                  <  SRR4302541.1963687/94‑1 (MQ=58)
                                              TTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAAC                                                 >  SRR4302541.1577527/1‑94 (MQ=60)
                                              TTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAAC                                                 >  SRR4302541.1624019/1‑94 (MQ=60)
                                              TTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAAC                                                 >  SRR4302541.1093086/1‑94 (MQ=60)
                                              TTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAAC                                                 <  SRR4302541.3544458/94‑1 (MQ=60)
                                              TTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAAC                                                 <  SRR4302541.3985423/94‑1 (MQ=60)
                                                TTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCC                                               <  SRR4302541.7966557/94‑1 (MQ=60)
                                                 TAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCA                                              >  SRR4302541.3956022/1‑94 (MQ=60)
                                                 TAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCA                                              >  SRR4302541.1390855/1‑94 (MQ=60)
                                                  AATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAA                                             <  SRR4302541.7472897/94‑1 (MQ=60)
                                                  AATATTTTGAACAATATGGCATTGAACGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAA                                             <  SRR4302541.8718205/94‑1 (MQ=60)
                                                   ATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAT                                            >  SRR4302541.2835931/1‑94 (MQ=60)
                                                   ATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAT                                            <  SRR4302541.6558304/94‑1 (MQ=60)
                                                   ATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAT                                            <  SRR4302541.7838531/94‑1 (MQ=60)
                                                   ATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAAT                                            >  SRR4302541.4387070/1‑94 (MQ=60)
                                                    TATTTTGAACAATATGGCGTTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATT                                           >  SRR4302541.2939215/1‑94 (MQ=60)
                                                    TATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATT                                           <  SRR4302541.5072268/94‑1 (MQ=60)
                                                    TATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATT                                           <  SRR4302541.4825023/94‑1 (MQ=60)
                                                    TATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATT                                           <  SRR4302541.6522027/94‑1 (MQ=60)
                                                    TATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATT                                           <  SRR4302541.1786247/94‑1 (MQ=60)
                                                    TATTATGAACAATATGGCATTGAATGATAAGAGCGCCTGCGCTCGAGGTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATT                                           <  SRR4302541.6522026/94‑1 (MQ=60)
                                                     ATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTT                                          <  SRR4302541.2224176/94‑1 (MQ=60)
                                                     ATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTT                                          <  SRR4302541.7239824/94‑1 (MQ=60)
                                                     ATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTT                                          <  SRR4302541.657896/94‑1 (MQ=60)
                                                     ATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTT                                          >  SRR4302541.4710291/1‑94 (MQ=60)
                                                     ATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTT                                          >  SRR4302541.4383386/1‑94 (MQ=60)
                                                     ATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTT                                          >  SRR4302541.8645953/1‑94 (MQ=60)
                                                     ATTTTGAACAATATGGCATTGAATGATAAGACCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTT                                          <  SRR4302541.30291/94‑1 (MQ=60)
                                                      TTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTA                                         <  SRR4302541.4489175/94‑1 (MQ=60)
                                                      TTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTA                                         <  SRR4302541.417604/94‑1 (MQ=60)
                                                       TTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTAT                                        >  SRR4302541.3802803/1‑94 (MQ=60)
                                                        TTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATT                                       >  SRR4302541.387431/1‑94 (MQ=60)
                                                        TTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATT                                       <  SRR4302541.5925038/94‑1 (MQ=60)
                                                        TTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTAAGCGATGCAAGAAAAACCCAATTTATT                                       >  SRR4302541.387430/1‑94 (MQ=60)
                                                         TGAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTC                                      >  SRR4302541.8272959/1‑94 (MQ=60)
                                                          GAACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGAGATGCAAGAAAAACCCAATTTATTCA                                     <  SRR4302541.779720/94‑1 (MQ=60)
                                                           AACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCAC                                    >  SRR4302541.4113040/1‑94 (MQ=60)
                                                           AACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCAC                                    <  SRR4302541.318959/94‑1 (MQ=60)
                                                           AACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCAC                                    <  SRR4302541.318958/94‑1 (MQ=60)
                                                           AACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCAC                                    <  SRR4302541.3566384/94‑1 (MQ=60)
                                                           AACAATATGGCAGTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCAC                                    <  SRR4302541.3566383/94‑1 (MQ=60)
                                                            ACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACC                                   <  SRR4302541.8760036/94‑1 (MQ=60)
                                                            ACAATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACC                                   <  SRR4302541.8760037/94‑1 (MQ=60)
                                                             CAATATGGCATTGAATAATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCT                                  <  SRR4302541.1575587/94‑1 (MQ=60)
                                                             CAATATGGCATTGAACGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCT                                  <  SRR4302541.1231663/94‑1 (MQ=60)
                                                              AATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTA                                 >  SRR4302541.2226959/1‑94 (MQ=60)
                                                               ATTTGGTATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCATCGGCTCAGCGTTGCAAGAAATACCCAATTTATTCACCTAA                                >  SRR4302541.7890124/1‑94 (MQ=60)
                                                               ATATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAA                                >  SRR4302541.2518568/1‑94 (MQ=60)
                                                                TATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAA                               >  SRR4302541.7670907/1‑94 (MQ=60)
                                                                 ATGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAA                              >  SRR4302541.1556335/1‑94 (MQ=60)
                                                                  TGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAT                             <  SRR4302541.8267176/94‑1 (MQ=60)
                                                                  TGGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAAT                             <  SRR4302541.6477908/94‑1 (MQ=60)
                                                                   GGCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATA                            <  SRR4302541.3916268/94‑1 (MQ=60)
                                                                    GCATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAG                           <  SRR4302541.492385/94‑1 (MQ=60)
                                                                     CATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACATAAAATAGT                          >  SRR4302541.1427755/1‑94 (MQ=60)
                                                                     CATTGAATAATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGT                          <  SRR4302541.3240658/94‑1 (MQ=60)
                                                                      ATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTA                         >  SRR4302541.8173258/1‑94 (MQ=60)
                                                                      ATTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTA                         >  SRR4302541.8398312/1‑94 (MQ=60)
                                                                       TTGAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAG                        >  SRR4302541.7671056/1‑94 (MQ=60)
                                                                         GAATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAA                      >  SRR4302541.4253476/1‑94 (MQ=60)
                                                                          AATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAAC                     <  SRR4302541.1665729/94‑1 (MQ=60)
                                                                          AATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAAC                     >  SRR4302541.3976443/1‑94 (MQ=60)
                                                                           ATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGGTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACA                    >  SRR4302541.5631355/1‑94 (MQ=60)
                                                                           ATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACA                    <  SRR4302541.4049288/94‑1 (MQ=60)
                                                                           ATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACA                    >  SRR4302541.4971258/1‑94 (MQ=60)
                                                                           ATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACA                    >  SRR4302541.5631356/1‑94 (MQ=60)
                                                                           ATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACA                    <  SRR4302541.269061/94‑1 (MQ=60)
                                                                           ATGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACA                    >  SRR4302541.7081584/1‑94 (MQ=60)
                                                                            TGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAA                   >  SRR4302541.6067126/1‑94 (MQ=60)
                                                                            TGATAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAA                   >  SRR4302541.3121384/1‑94 (MQ=60)
                                                                               TAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCT                <  SRR4302541.610076/94‑1 (MQ=60)
                                                                               TAAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCT                >  SRR4302541.7286162/1‑94 (MQ=60)
                                                                                AAGAGCGCTTGTGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTC               <  SRR4302541.1021229/94‑1 (MQ=60)
                                                                                AAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTC               >  SRR4302541.5866539/1‑94 (MQ=60)
                                                                                AAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTC               <  SRR4302541.534202/94‑1 (MQ=60)
                                                                                AAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTC               >  SRR4302541.2389835/1‑94 (MQ=60)
                                                                                AAGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTC               >  SRR4302541.4124987/1‑94 (MQ=60)
                                                                                 AGAGCGCTTGCGCTCGAGCTACGGCAATCGGGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAACTAGTAGCACAATCTCA              >  SRR4302541.3551778/1‑94 (MQ=60)
                                                                                 AGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCA              <  SRR4302541.5257357/94‑1 (MQ=60)
                                                                                 AGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCA              <  SRR4302541.3083299/94‑1 (MQ=60)
                                                                                 AGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCA              <  SRR4302541.6684773/94‑1 (MQ=60)
                                                                                 AGAGCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCA              >  SRR4302541.1410662/1‑94 (MQ=60)
                                                                                    GCGCTTGCGCTCGAGCTACGGCAATCGCGTCAACGGCTCAGCGATGCAAGAGAAACCCAATTTATTAACCTAAAATAGTAGAACAATCTCAAAT           >  SRR4302541.5056160/1‑94 (MQ=60)
                                                                                    GCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAAT           <  SRR4302541.3721472/94‑1 (MQ=60)
                                                                                    GCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAAT           <  SRR4302541.4611274/94‑1 (MQ=60)
                                                                                    GCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAAT           >  SRR4302541.7612751/1‑94 (MQ=60)
                                                                                    GCGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAAT           <  SRR4302541.1830211/94‑1 (MQ=60)
                                                                                     CGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATT          >  SRR4302541.2050335/1‑94 (MQ=60)
                                                                                     CGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATT          <  SRR4302541.6997110/94‑1 (MQ=60)
                                                                                     CGCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATT          <  SRR4302541.264227/94‑1 (MQ=60)
                                                                                      GCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTA         >  SRR4302541.7782837/1‑94 (MQ=60)
                                                                                      GCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTA         >  SRR4302541.264696/1‑94 (MQ=60)
                                                                                      GCTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTA         >  SRR4302541.6032197/1‑94 (MQ=60)
                                                                                       CTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCCATTTATTCACCTAAAATAGTAGAACAATCTCAAATTAT        >  SRR4302541.6187374/1‑94 (MQ=60)
                                                                                       CTTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTAC        <  SRR4302541.5157098/94‑1 (MQ=60)
                                                                                        TTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACT       >  SRR4302541.8759159/1‑94 (MQ=60)
                                                                                        TTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACT       <  SRR4302541.3819614/94‑1 (MQ=60)
                                                                                        TTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACT       <  SRR4302541.3819613/94‑1 (MQ=60)
                                                                                        TTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACT       <  SRR4302541.3730590/94‑1 (MQ=60)
                                                                                        TTGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACT       <  SRR4302541.5746585/94‑1 (MQ=60)
                                                                                         TGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTG      <  SRR4302541.2838137/94‑1 (MQ=60)
                                                                                         TGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTG      <  SRR4302541.7491953/94‑1 (MQ=60)
                                                                                         TGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTG      <  SRR4302541.8877664/94‑1 (MQ=60)
                                                                                         TGCGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTG      <  SRR4302541.7486134/94‑1 (MQ=60)
                                                                                          GCGCTCGAGCTACGGCAATCGAGTCAACGGCTTAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTAC     <  SRR4302541.1011965/94‑1 (MQ=60)
                                                                                           CGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATaatgtca    >  SRR4302541.2892179/1‑87 (MQ=60)
                                                                                           CGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCT    >  SRR4302541.5728767/1‑94 (MQ=60)
                                                                                           CGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCT    >  SRR4302541.2647858/1‑94 (MQ=60)
                                                                                           CGCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCT    <  SRR4302541.1868797/94‑1 (MQ=60)
                                                                                            GCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTT   >  SRR4302541.2455229/1‑94 (MQ=60)
                                                                                            GCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTT   >  SRR4302541.2266658/1‑94 (MQ=60)
                                                                                            GCTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTT   >  SRR4302541.3318976/1‑94 (MQ=60)
                                                                                             CTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTT  >  SRR4302541.2324410/1‑94 (MQ=60)
                                                                                             CTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTT  <  SRR4302541.3029888/94‑1 (MQ=60)
                                                                                             |                                                                                             
TTATACTCGGGCAAATAAAAATTTGCAAAAAATGCGAAGATAATGTTTTTAATATTTTGAACAATATGGCATTGAATGATAAGAGCGCTTGCGTTCGAGCTACGGCAATCGAGTCAACGGCTCAGCGATGCAAGAAAAACCCAATTTATTCACCTAAAATAGTAGAACAATCTCAAATTACTGCTTT  >  NC_000913/3764190‑3764376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑