Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,854,698 T→C Y445C (TAC→TGC)  yidJ ← putative sulfatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,854,6980TC99.5% 1324.4 / ‑3.9 384Y445C (TAC→TGC) yidJputative sulfatase
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (178/204);  total (180/204)
Fisher's exact test for biased strand distribution p-value = 2.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.26e-01

Only 100 of 395 total aligned reads displayed.

ACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCG  >  NC_000913/3854608‑3854787
                                                                                          |                                                                                         
aCTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCaa                                                                                         <  2:7143441/93‑1 (MQ=255)
 cTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAAc                                                                                        <  2:1905210/93‑1 (MQ=255)
 cTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAAc                                                                                        <  2:573412/93‑1 (MQ=255)
   ggCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTg                                                                                      >  1:509036/1‑93 (MQ=255)
     cGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCg                                                                                    <  2:302470/93‑1 (MQ=255)
      gAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCgg                                                                                   >  2:8205993/1‑93 (MQ=255)
       aaTAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGa                                                                                  >  1:5047305/1‑93 (MQ=255)
       aaTAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGa                                                                                  <  2:1731747/93‑1 (MQ=255)
        aTAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGaa                                                                                 <  1:7248259/93‑1 (MQ=255)
        aTAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGaa                                                                                 >  2:3633544/1‑93 (MQ=255)
        aTAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGaa                                                                                 >  2:4913082/1‑93 (MQ=255)
         tAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAAc                                                                                <  1:7309616/93‑1 (MQ=255)
          aGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACg                                                                               <  1:1835654/93‑1 (MQ=255)
          aGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACg                                                                               >  2:525783/1‑93 (MQ=255)
            ccATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGa                                                                             <  1:113800/93‑1 (MQ=255)
            ccATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGa                                                                             <  2:768775/93‑1 (MQ=255)
            ccATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGa                                                                             <  1:131255/93‑1 (MQ=255)
              aTCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATc                                                                           >  2:6064861/1‑93 (MQ=255)
              aTCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATc                                                                           >  2:6936433/1‑93 (MQ=255)
                 ttGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATcgcg                                                                        >  1:2207642/1‑93 (MQ=255)
                 ttGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATcgcg                                                                        <  1:5609669/93‑1 (MQ=255)
                  tgtgGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGa                                                                       >  2:6567676/1‑93 (MQ=255)
                   gtggacgtggacGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGaa                                                                      <  1:3872222/93‑1 (MQ=255)
                   gtggacgtggacGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGaa                                                                      <  1:4933365/93‑1 (MQ=255)
                   gtggacgtggacGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGaa                                                                      <  1:1419583/93‑1 (MQ=255)
                    gggacgtggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAt                                                                     <  1:3063097/92‑1 (MQ=255)
                     ggacgtggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAtt                                                                    >  2:2199005/1‑93 (MQ=255)
                          tggacgAAACGCCCCCATCCAGCGCGGCCGTGCTTCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTc                                                               <  1:8523761/93‑1 (MQ=255)
                          tggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTc                                                               >  2:121791/1‑93 (MQ=255)
                            gacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCa                                                             <  1:2735478/93‑1 (MQ=255)
                              cgAAACTCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACGCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATg                                                           <  1:1575307/93‑1 (MQ=255)
                               gAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGt                                                          <  2:3856761/93‑1 (MQ=255)
                                aaaCGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTa                                                         >  1:756243/1‑93 (MQ=255)
                                aaaCGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGAGCATTTTGTCCATGTa                                                         >  2:3898335/1‑93 (MQ=255)
                                 aaCGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTaa                                                        <  2:1380209/93‑1 (MQ=255)
                                  aCGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAAt                                                       <  1:6633600/93‑1 (MQ=255)
                                   cGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATc                                                      >  2:2947128/1‑93 (MQ=255)
                                     cccccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCa                                                    >  1:5403257/1‑93 (MQ=255)
                                      ccccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCaa                                                   <  1:2478218/93‑1 (MQ=255)
                                      ccccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCaa                                                   >  1:4364840/1‑93 (MQ=255)
                                      ccccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCaa                                                   >  1:1967430/1‑93 (MQ=255)
                                       cccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCCAt                                                  >  1:6001448/1‑93 (MQ=255)
                                       cccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAAt                                                  >  2:7367926/1‑93 (MQ=255)
                                       cccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAAt                                                  <  1:8663787/93‑1 (MQ=255)
                                        ccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCCATa                                                 >  2:8347947/1‑93 (MQ=255)
                                        ccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATa                                                 >  1:6597038/1‑93 (MQ=255)
                                         cATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATaa                                                <  2:6321540/93‑1 (MQ=255)
                                         cATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATaa                                                <  1:5655989/93‑1 (MQ=255)
                                          aTCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAg                                               >  1:6126613/1‑93 (MQ=255)
                                           tCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAgg                                              <  1:4061434/93‑1 (MQ=255)
                                           tCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAgg                                              >  1:6223155/1‑93 (MQ=255)
                                           tCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAgg                                              >  1:7148120/1‑93 (MQ=255)
                                             cAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCg                                            <  2:4883789/93‑1 (MQ=255)
                                             cAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCg                                            <  1:4656530/93‑1 (MQ=255)
                                              aGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGt                                           >  1:3611975/1‑93 (MQ=255)
                                               gcgcGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTc                                          >  1:1029310/1‑93 (MQ=255)
                                               gcgcGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGGCCATGTTATCCAATAACGCGTc                                          >  2:2668493/1‑93 (MQ=255)
                                                cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCa                                         >  2:8580255/1‑93 (MQ=255)
                                                cgcgGCCGTGCATCTTTACGCCACGGACCCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCa                                         <  2:2622127/93‑1 (MQ=255)
                                                 gcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCTAATTTTGTCCATGGAATCCAAAAAGGCGTCAt                                        >  2:6670510/1‑93 (MQ=255)
                                                 gcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCAt                                        <  2:4676980/93‑1 (MQ=255)
                                                   ggCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGc                                      >  1:4763273/1‑93 (MQ=255)
                                                    gCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCa                                     <  1:2942063/93‑1 (MQ=255)
                                                     ccGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAt                                    >  2:6179915/1‑93 (MQ=255)
                                                      cGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAtt                                   <  1:7014291/93‑1 (MQ=255)
                                                      cGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAtt                                   <  1:8262792/93‑1 (MQ=255)
                                                       gTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAttt                                  <  2:272351/93‑1 (MQ=255)
                                                       gTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAttt                                  >  2:3701385/1‑93 (MQ=255)
                                                        tGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAtttt                                 >  1:1659012/1‑93 (MQ=255)
                                                        tGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAtttt                                 >  2:1846536/1‑93 (MQ=255)
                                                        tGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAtttt                                 <  1:5868090/93‑1 (MQ=255)
                                                          cATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTtgt                               >  1:7972445/1‑92 (MQ=255)
                                                            tCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTtgctg                             >  2:3502463/1‑93 (MQ=255)
                                                            tCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTtgctg                             >  1:681560/1‑93 (MQ=255)
                                                             cTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTtgctgc                            <  2:4487240/93‑1 (MQ=255)
                                                              tttACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCg                           >  1:3796897/1‑93 (MQ=255)
                                                                tACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGaa                         <  1:8047736/93‑1 (MQ=255)
                                                                 aCGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAAc                        <  2:8601335/93‑1 (MQ=255)
                                                                    ccACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTc                     <  1:6464996/93‑1 (MQ=255)
                                                                      aCGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTg                   >  2:1464080/1‑93 (MQ=255)
                                                                        ggACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCa                 <  2:5934164/93‑1 (MQ=255)
                                                                           cccAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCaaaa              <  1:8137717/91‑1 (MQ=255)
                                                                            gCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAAc             >  1:6363776/1‑93 (MQ=255)
                                                                            gCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAAc             <  2:7596783/93‑1 (MQ=255)
                                                                               gACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGGATTTTGCTGCGAACGTCTGCAAAACGGa          >  1:7753371/1‑93 (MQ=255)
                                                                               gACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGa          <  1:5786767/93‑1 (MQ=255)
                                                                                aCTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACggct         >  2:255975/1‑91 (MQ=255)
                                                                                aCTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGat         <  2:3448585/93‑1 (MQ=255)
                                                                                aCTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGat         >  2:7850507/1‑93 (MQ=255)
                                                                                aCTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGat         <  1:945971/93‑1 (MQ=255)
                                                                                aCTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGat         >  1:4886367/1‑93 (MQ=255)
                                                                                 cTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGata        <  1:2388632/93‑1 (MQ=255)
                                                                                 cTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGata        <  2:900040/93‑1 (MQ=255)
                                                                                  tCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGCACGTCTGCAAAACGGatat       >  1:6513222/1‑93 (MQ=255)
                                                                                   ccATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatc      <  2:5480917/93‑1 (MQ=255)
                                                                                   ccATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatc      <  2:7552798/93‑1 (MQ=255)
                                                                                    cATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatca     >  1:3140701/1‑93 (MQ=255)
                                                                                     aTTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatcat    >  2:6825142/1‑93 (MQ=255)
                                                                                      ttGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatcatc   <  2:4265297/93‑1 (MQ=255)
                                                                                       tGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCg  <  2:4147034/93‑1 (MQ=255)
                                                                                          |                                                                                         
ACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCG  >  NC_000913/3854608‑3854787

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 225 total aligned reads displayed.
CAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCA  >  NC_000913/3854606‑3854791
                                                                                            |                                                                                             
CAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCA                                                                                              >  SRR4302541.6823997/1‑94 (MQ=60)
 AACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAA                                                                                             >  SRR4302541.6479386/1‑94 (MQ=60)
   CTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACT                                                                                           >  SRR4302541.49430/1‑94 (MQ=60)
   CTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACT                                                                                           <  SRR4302541.4873926/94‑1 (MQ=60)
   CTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACT                                                                                           >  SRR4302541.6883633/1‑94 (MQ=60)
    TGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTG                                                                                          >  SRR4302541.513801/1‑94 (MQ=60)
    TGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTG                                                                                          >  SRR4302541.6816937/1‑94 (MQ=60)
       CGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGG                                                                                       >  SRR4302541.8222767/1‑94 (MQ=60)
        GAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGA                                                                                      >  SRR4302541.5904261/1‑94 (MQ=60)
        GAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGA                                                                                      >  SRR4302541.5099244/1‑94 (MQ=60)
         AATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAA                                                                                     >  SRR4302541.7973655/1‑94 (MQ=60)
          ATAGCCATCTTGTGGACGTGGACGAAACGGCCCCATCCAACGCGGCCGTGCATCTTTACGCCACGGACGCAGGCTCCATTGGCAACTGCGGAAC                                                                                    <  SRR4302541.8298341/94‑1 (MQ=60)
          ATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAAC                                                                                    <  SRR4302541.8209941/94‑1 (MQ=60)
          ATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAAC                                                                                    <  SRR4302541.8676042/94‑1 (MQ=60)
          ATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTCGCAACTGCGCAAC                                                                                    >  SRR4302541.7281438/1‑94 (MQ=60)
           TAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACG                                                                                   <  SRR4302541.7387648/94‑1 (MQ=60)
            AGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGgcgagcag                                                                                  >  SRR4302541.1971745/1‑86 (MQ=60)
            AGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGG                                                                                  <  SRR4302541.1853449/94‑1 (MQ=60)
              CCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGAT                                                                                <  SRR4302541.6415377/94‑1 (MQ=60)
              CCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGAT                                                                                <  SRR4302541.132286/94‑1 (MQ=60)
              CCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGAT                                                                                <  SRR4302541.114724/94‑1 (MQ=60)
               CATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATC                                                                               <  SRR4302541.5944253/94‑1 (MQ=60)
               CATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATC                                                                               >  SRR4302541.2281045/1‑94 (MQ=60)
               CATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATC                                                                               <  SRR4302541.5892338/94‑1 (MQ=60)
               CATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCAAGGACGCAGACTCCATTGGCAACTGCGGAACGGATC                                                                               <  SRR4302541.3867401/94‑1 (MQ=60)
               CATCTGGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATC                                                                               <  SRR4302541.3120322/94‑1 (MQ=60)
                ATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGCCGCAGAATCCATTGGCAACTGCGcaacgcctcc                                                                              >  SRR4302541.5095067/1‑84 (MQ=60)
                ATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCG                                                                              >  SRR4302541.1458294/1‑94 (MQ=60)
                 TCTTGTGGACGTGGACGAAACGCACCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGC                                                                             <  SRR4302541.1736799/94‑1 (MQ=60)
                  CTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCG                                                                            >  SRR4302541.5109879/1‑94 (MQ=60)
                  CTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCG                                                                            >  SRR4302541.2229035/1‑94 (MQ=60)
                  CTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCG                                                                            <  SRR4302541.6570504/94‑1 (MQ=60)
                  CTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCG                                                                            >  SRR4302541.6012550/1‑94 (MQ=60)
                   TTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGA                                                                           <  SRR4302541.5667086/94‑1 (MQ=60)
                    TGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAA                                                                          >  SRR4302541.1505741/1‑94 (MQ=60)
                     GTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAT                                                                         <  SRR4302541.6154324/94‑1 (MQ=60)
                     GTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAT                                                                         <  SRR4302541.3911154/94‑1 (MQ=60)
                     GTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAT                                                                         <  SRR4302541.1433402/94‑1 (MQ=60)
                     GTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAT                                                                         <  SRR4302541.4983703/94‑1 (MQ=60)
                      TGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATT                                                                        >  SRR4302541.4802728/1‑94 (MQ=60)
                       GGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTT                                                                       >  SRR4302541.2105974/1‑94 (MQ=60)
                       GGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTT                                                                       >  SRR4302541.5285237/1‑94 (MQ=60)
                         ACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTG                                                                     >  SRR4302541.56678/1‑94 (MQ=60)
                         ACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTG                                                                     >  SRR4302541.3097215/1‑94 (MQ=60)
                          CGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGT                                                                    <  SRR4302541.4469430/94‑1 (MQ=60)
                            TGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCC                                                                  <  SRR4302541.8507755/94‑1 (MQ=60)
                            TGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCC                                                                  >  SRR4302541.2153502/1‑94 (MQ=60)
                            TGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCC                                                                  >  SRR4302541.6242780/1‑94 (MQ=60)
                             GGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCA                                                                 >  SRR4302541.2452194/1‑94 (MQ=60)
                             GGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCA                                                                 <  SRR4302541.5118974/94‑1 (MQ=60)
                             GGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCA                                                                 >  SRR4302541.766191/1‑94 (MQ=60)
                                CGAAACTCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACGCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGT                                                              <  SRR4302541.1591055/94‑1 (MQ=60)
                                CGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGT                                                              >  SRR4302541.2701188/1‑94 (MQ=60)
                                CGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGT                                                              >  SRR4302541.2769712/1‑94 (MQ=60)
                                 GAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTA                                                             <  SRR4302541.7684894/94‑1 (MQ=60)
                                 GAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTA                                                             <  SRR4302541.863755/94‑1 (MQ=60)
                                 GAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTA                                                             <  SRR4302541.8235622/94‑1 (MQ=60)
                                 GAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTA                                                             >  SRR4302541.1986202/1‑94 (MQ=60)
                                  AAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGAGTCCATTGTCAACTGCGGAACGGAACGCGCATTTTGgccattgaa                                                            >  SRR4302541.7262714/1‑85 (MQ=60)
                                  AAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAA                                                            <  SRR4302541.8080423/94‑1 (MQ=60)
                                  AAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAA                                                            <  SRR4302541.5756562/94‑1 (MQ=60)
                                   AACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAAT                                                           <  SRR4302541.4937631/94‑1 (MQ=60)
                                   AACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGAAGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCCTGTAAG                                                           >  SRR4302541.2993002/1‑94 (MQ=60)
                                    ACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATC                                                          <  SRR4302541.6701607/94‑1 (MQ=60)
                                     CGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCC                                                         >  SRR4302541.6646962/1‑94 (MQ=60)
                                      GCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATTCA                                                        >  SRR4302541.4010360/1‑94 (MQ=60)
                                      GCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCA                                                        <  SRR4302541.7328831/94‑1 (MQ=60)
                                      GCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCA                                                        >  SRR4302541.5458698/1‑94 (MQ=60)
                                      CCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCA                                                        <  SRR4302541.1244870/94‑1 (MQ=60)
                                       CCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGCATTTTGTCCATGTAATCCAA                                                       >  SRR4302541.8760393/1‑94 (MQ=60)
                                       CCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTTTCCATGTAATCCAA                                                       >  SRR4302541.2759287/1‑94 (MQ=60)
                                       CCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAA                                                       >  SRR4302541.1986406/1‑94 (MQ=60)
                                       CCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAA                                                       >  SRR4302541.7705049/1‑94 (MQ=60)
                                       CCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAA                                                       >  SRR4302541.4408455/1‑94 (MQ=60)
                                       CCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAA                                                       >  SRR4302541.7431393/1‑94 (MQ=60)
                                        CCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCCAT                                                      >  SRR4302541.6062927/1‑94 (MQ=60)
                                        CCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAAT                                                      <  SRR4302541.2503068/94‑1 (MQ=60)
                                        CCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAAA                                                      >  SRR4302541.4156912/1‑94 (MQ=60)
                                        CCCCAGCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAAT                                                      <  SRR4302541.8747338/94‑1 (MQ=60)
                                         CCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATA                                                     >  SRR4302541.6664598/1‑94 (MQ=60)
                                         CCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATA                                                     >  SRR4302541.7224417/1‑94 (MQ=60)
                                         CCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATA                                                     >  SRR4302541.6935660/1‑94 (MQ=60)
                                         CCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATA                                                     >  SRR4302541.1674137/1‑94 (MQ=60)
                                         CCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATA                                                     <  SRR4302541.8756513/94‑1 (MQ=60)
                                         CCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCAGGTAATCCAATA                                                     >  SRR4302541.6664597/1‑94 (MQ=60)
                                          CCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAA                                                    >  SRR4302541.8269705/1‑94 (MQ=60)
                                          CCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAA                                                    <  SRR4302541.3048131/94‑1 (MQ=60)
                                           CATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGGATTTTGTACATGGAATCCTATAAG                                                   >  SRR4302541.3281711/1‑94 (MQ=60)
                                           CATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAG                                                   <  SRR4302541.8189088/94‑1 (MQ=60)
                                           CATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAG                                                   >  SRR4302541.1226722/1‑94 (MQ=60)
                                           CATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAG                                                   >  SRR4302541.6189179/1‑94 (MQ=60)
                                           CATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAG                                                   <  SRR4302541.5713756/94‑1 (MQ=60)
                                           CATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAG                                                   >  SRR4302541.6604241/1‑94 (MQ=60)
                                            ATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGG                                                  >  SRR4302541.6286739/1‑94 (MQ=60)
                                            ATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGG                                                  >  SRR4302541.7058/1‑94 (MQ=60)
                                            ATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGG                                                  >  SRR4302541.7224314/1‑94 (MQ=60)
                                             TCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGC                                                 >  SRR4302541.4120955/1‑94 (MQ=60)
                                             TCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGC                                                 <  SRR4302541.4101512/94‑1 (MQ=60)
                                             TCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGC                                                 >  SRR4302541.6572015/1‑94 (MQ=60)
                                             TCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGC                                                 <  SRR4302541.2971947/94‑1 (MQ=60)
                                              CCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCG                                                <  SRR4302541.5464637/94‑1 (MQ=60)
                                               CAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGT                                               >  SRR4302541.3648591/1‑94 (MQ=60)
                                               CAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGT                                               <  SRR4302541.4703943/94‑1 (MQ=60)
                                                AGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTC                                              <  SRR4302541.5696195/94‑1 (MQ=60)
                                                AGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTC                                              >  SRR4302541.1038813/1‑94 (MQ=60)
                                                 GCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCA                                             >  SRR4302541.6414428/1‑94 (MQ=60)
                                                  CGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCAT                                            <  SRR4302541.3675624/94‑1 (MQ=60)
                                                   GCGGCCGTGCATCTTTACGCCACGGACGGAGACTGCATTGGCAACTGCGGAACGGGTCGCGGATTTTGTCCATGTAATCCAATAAGGCGTGATG                                           >  SRR4302541.3142815/1‑94 (MQ=60)
                                                   GCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCCTG                                           >  SRR4302541.5033375/1‑94 (MQ=60)
                                                   GCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATG                                           <  SRR4302541.3170486/94‑1 (MQ=60)
                                                    CGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGC                                          >  SRR4302541.4811921/1‑94 (MQ=60)
                                                     GGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCA                                         >  SRR4302541.7153449/1‑94 (MQ=60)
                                                      GCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAT                                        <  SRR4302541.2971654/94‑1 (MQ=60)
                                                      GCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAT                                        <  SRR4302541.3861479/94‑1 (MQ=60)
                                                        CGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTT                                      <  SRR4302541.8352749/94‑1 (MQ=60)
                                                        CGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTT                                      <  SRR4302541.7088892/94‑1 (MQ=60)
                                                         GTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGCATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTT                                     >  SRR4302541.7307196/1‑94 (MQ=60)
                                                         GTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTT                                     >  SRR4302541.3697162/1‑94 (MQ=60)
                                                         GTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTT                                     >  SRR4302541.6555050/1‑94 (MQ=60)
                                                         GTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTT                                     >  SRR4302541.1675380/1‑94 (MQ=60)
                                                          TGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTG                                    <  SRR4302541.5927787/94‑1 (MQ=60)
                                                          TGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTG                                    >  SRR4302541.8120075/1‑94 (MQ=60)
                                                           GCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGT                                   >  SRR4302541.8060293/1‑94 (MQ=60)
                                                           GCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGC                                   >  SRR4302541.1075832/1‑94 (MQ=60)
                                                            CATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCT                                  >  SRR4302541.1640819/1‑94 (MQ=60)
                                                             ATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTG                                 >  SRR4302541.1737553/1‑94 (MQ=60)
                                                             ATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTG                                 >  SRR4302541.687992/1‑94 (MQ=60)
                                                              TCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGC                                <  SRR4302541.793499/94‑1 (MQ=60)
                                                               CTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCG                               <  SRR4302541.1738109/94‑1 (MQ=60)
                                                               CTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCG                               >  SRR4302541.480154/1‑94 (MQ=60)
                                                               CTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCG                               >  SRR4302541.3835104/1‑94 (MQ=60)
                                                                TTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGA                              >  SRR4302541.1386067/1‑94 (MQ=60)
                                                                 TTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAA                             <  SRR4302541.4003904/94‑1 (MQ=60)
                                                                  TACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAAC                            >  SRR4302541.6475277/1‑94 (MQ=60)
                                                                  TACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAAC                            <  SRR4302541.8136226/94‑1 (MQ=60)
                                                                  TACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAAC                            <  SRR4302541.6669530/94‑1 (MQ=60)
                                                                    CGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGT                          >  SRR4302541.6103138/1‑94 (MQ=60)
                                                                    CGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGT                          <  SRR4302541.2279402/94‑1 (MQ=60)
                                                                     GCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTTCATGTAATCCAATAAGGCGTCATGCATTTTGCTGagaaccac                         >  SRR4302541.6994833/1‑86 (MQ=60)
                                                                     GCCACGAACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTC                         >  SRR4302541.291380/1‑94 (MQ=60)
                                                                      CCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCT                        <  SRR4302541.6530875/94‑1 (MQ=60)
                                                                      CCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCT                        <  SRR4302541.5593973/94‑1 (MQ=60)
                                                                      CCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCT                        >  SRR4302541.5946737/1‑94 (MQ=60)
                                                                      CCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCT                        <  SRR4302541.6242799/94‑1 (MQ=60)
                                                                       CACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGcctc                       >  SRR4302541.5763015/1‑90 (MQ=60)
                                                                       CACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTG                       <  SRR4302541.4471983/94‑1 (MQ=60)
                                                                         CGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCA                     <  SRR4302541.8384625/94‑1 (MQ=60)
                                                                          CGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAA                    <  SRR4302541.7355057/94‑1 (MQ=60)
                                                                           GACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAA                   <  SRR4302541.5550608/94‑1 (MQ=60)
                                                                            ACGCAGACTCCATTGGCAACTGCGGAACGGCTCGCGAATTTTGTCCCTGTAATCCAATAAGGGGTCATGCATTTTGCTGCGAACGctcgcaaca                  >  SRR4302541.7484437/1‑85 (MQ=60)
                                                                            ACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAA                  >  SRR4302541.7484436/1‑94 (MQ=60)
                                                                             CGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCCTGCATTTTCCTGCGAACGTCTGCAAAAC                 >  SRR4302541.1947416/1‑94 (MQ=60)
                                                                             CGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAAC                 >  SRR4302541.6428843/1‑94 (MQ=60)
                                                                              GCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACG                >  SRR4302541.90335/1‑94 (MQ=60)
                                                                              GCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGACAAACG                >  SRR4302541.2451247/1‑94 (MQ=60)
                                                                               CAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGG               >  SRR4302541.6460258/1‑94 (MQ=60)
                                                                               CAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGG               <  SRR4302541.3699668/94‑1 (MQ=60)
                                                                                CGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGA              <  SRR4302541.6684820/94‑1 (MQ=60)
                                                                                AGACTCCATTGGCAACTGCGGAACGTATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGA              <  SRR4302541.1294881/94‑1 (MQ=60)
                                                                                AGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGGATTTTGCTGCGAACGTCTGCAAAACGGA              >  SRR4302541.7835854/1‑94 (MQ=60)
                                                                                AGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGA              <  SRR4302541.6684580/94‑1 (MQ=60)
                                                                                AGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGA              >  SRR4302541.5076387/1‑94 (MQ=60)
                                                                                 GACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGAT             <  SRR4302541.392018/94‑1 (MQ=60)
                                                                                 GACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGAT             >  SRR4302541.4936232/1‑94 (MQ=60)
                                                                                 GACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGAT             <  SRR4302541.149263/94‑1 (MQ=60)
                                                                                 GACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGAT             <  SRR4302541.5845578/94‑1 (MQ=60)
                                                                                 GACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGAT             >  SRR4302541.4956776/1‑94 (MQ=60)
                                                                                 GACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGAT             <  SRR4302541.2841330/94‑1 (MQ=60)
                                                                                  GCTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATA            <  SRR4302541.2479039/94‑1 (MQ=60)
                                                                                  ACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATA            <  SRR4302541.4411913/94‑1 (MQ=60)
                                                                                  ACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATA            <  SRR4302541.7367848/94‑1 (MQ=60)
                                                                                   CTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATAT           <  SRR4302541.3298781/94‑1 (MQ=60)
                                                                                   CTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATAT           <  SRR4302541.2411997/94‑1 (MQ=60)
                                                                                   CTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATAT           <  SRR4302541.8876374/94‑1 (MQ=60)
                                                                                   CTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATAT           <  SRR4302541.8076554/94‑1 (MQ=60)
                                                                                    TCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATC          >  SRR4302541.7092740/1‑94 (MQ=60)
                                                                                    TCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATC          >  SRR4302541.1064966/1‑94 (MQ=60)
                                                                                     CCATTGGCAACTGCGGAACGGATCTCGAATTTTGTCCATGTAATCCAATAAGCCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCA         >  SRR4302541.2180771/1‑94 (MQ=60)
                                                                                     CCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAATGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCA         >  SRR4302541.8367807/1‑94 (MQ=60)
                                                                                     CCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCA         >  SRR4302541.3172428/1‑94 (MQ=60)
                                                                                     CCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCA         >  SRR4302541.5395911/1‑94 (MQ=60)
                                                                                      CATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCAT        >  SRR4302541.4924701/1‑94 (MQ=60)
                                                                                      CATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCAT        <  SRR4302541.573564/94‑1 (MQ=60)
                                                                                        TTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCG      <  SRR4302541.1404950/94‑1 (MQ=60)
                                                                                        TTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCG      <  SRR4302541.5106890/94‑1 (MQ=60)
                                                                                        TTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCG      <  SRR4302541.757189/94‑1 (MQ=60)
                                                                                         TGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGA     <  SRR4302541.259816/94‑1 (MQ=60)
                                                                                         TGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGA     <  SRR4302541.6276952/94‑1 (MQ=60)
                                                                                         TGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGA     >  SRR4302541.3351332/1‑94 (MQ=60)
                                                                                          GGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAT    <  SRR4302541.7974903/94‑1 (MQ=60)
                                                                                          GGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAT    <  SRR4302541.8631103/94‑1 (MQ=60)
                                                                                          GGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAT    >  SRR4302541.7686100/1‑94 (MQ=60)
                                                                                          GGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAT    >  SRR4302541.603003/1‑94 (MQ=60)
                                                                                          GGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAT    <  SRR4302541.3917906/94‑1 (MQ=60)
                                                                                           GCAACTGCTGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATC   <  SRR4302541.6098282/94‑1 (MQ=60)
                                                                                           GCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATC   >  SRR4302541.2809273/1‑94 (MQ=60)
                                                                                           GCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATC   <  SRR4302541.8291615/94‑1 (MQ=60)
                                                                                           GCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATC   <  SRR4302541.5497356/94‑1 (MQ=60)
                                                                                           GCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATC   <  SRR4302541.3769099/94‑1 (MQ=60)
                                                                                            CAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCA  >  SRR4302541.6889429/1‑94 (MQ=60)
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CAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATCA  >  NC_000913/3854606‑3854791

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑