Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,854,698 T→C Y445C (TAC→TGC)  yidJ ← putative sulfatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,854,6980TC99.7% 1002.2 / ‑5.2 282Y445C (TAC→TGC) yidJputative sulfatase
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (124/157);  total (125/157)
Fisher's exact test for biased strand distribution p-value = 4.43e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 286 total aligned reads displayed.

ACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAT  >  NC_000913/3854608‑3854789
                                                                                          |                                                                                           
 cTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAAc                                                                                          <  2:3424986/93‑1 (MQ=255)
 cTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAAc                                                                                          >  2:2923600/1‑93 (MQ=255)
  tGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCCTTGGCAACt                                                                                         >  1:1029846/1‑93 (MQ=255)
   ggCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTg                                                                                        >  1:243797/1‑93 (MQ=255)
       aaTAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGa                                                                                    >  2:1005649/1‑93 (MQ=255)
        aTAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGaa                                                                                   >  1:380191/1‑93 (MQ=255)
         tAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAAc                                                                                  <  2:771103/93‑1 (MQ=255)
           gCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACgg                                                                                <  2:937948/93‑1 (MQ=255)
           gCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACgg                                                                                <  2:6093517/93‑1 (MQ=255)
            ccATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGa                                                                               >  2:3648183/1‑93 (MQ=255)
             cATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGAt                                                                              <  2:2462625/93‑1 (MQ=255)
             cATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGAt                                                                              <  2:6144613/93‑1 (MQ=255)
              aTCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATc                                                                             <  2:6207440/93‑1 (MQ=255)
              aTCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATc                                                                             >  1:1723933/1‑93 (MQ=255)
                cTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATcgc                                                                           <  1:742995/93‑1 (MQ=255)
                 ttGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATcgcg                                                                          <  1:5765436/93‑1 (MQ=255)
                  tgtgGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGa                                                                         >  2:1864113/1‑93 (MQ=255)
                     ggacgtggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAtt                                                                      <  2:6323297/93‑1 (MQ=255)
                      gacgtggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAttt                                                                     <  1:2852564/93‑1 (MQ=255)
                      gacgtggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAttt                                                                     <  1:3289005/93‑1 (MQ=255)
                      gacgtggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAttt                                                                     <  1:519838/93‑1 (MQ=255)
                           ggacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCCAATTTTGTcc                                                                >  2:310656/1‑93 (MQ=255)
                            gacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCa                                                               >  2:3939797/1‑93 (MQ=255)
                            gacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCa                                                               <  2:2016376/93‑1 (MQ=255)
                            gacgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCa                                                               >  1:2492927/1‑93 (MQ=255)
                              cgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATg                                                             <  1:3927901/93‑1 (MQ=255)
                              cgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATg                                                             <  1:1197316/93‑1 (MQ=255)
                              cgAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATg                                                             <  2:1154606/93‑1 (MQ=255)
                                aaaCGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTTCATGTa                                                           >  1:5643361/1‑93 (MQ=255)
                                 aaCGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTaa                                                          <  2:598946/93‑1 (MQ=255)
                                 aaCGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTaa                                                          <  2:5360154/93‑1 (MQ=255)
                                 aaCGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTaa                                                          >  2:6396026/1‑93 (MQ=255)
                                   cGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATc                                                        <  2:3538144/93‑1 (MQ=255)
                                   cGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATc                                                        <  1:4431673/93‑1 (MQ=255)
                                      ccccATCCAGCTCGGCCGTGCATCTTTACGCCGCGGACTCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCaa                                                     <  2:529577/93‑1 (MQ=255)
                                      ccccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCaa                                                     >  2:663638/1‑93 (MQ=255)
                                       cccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAAt                                                    <  2:2562580/93‑1 (MQ=255)
                                       cccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAAt                                                    <  1:2337045/93‑1 (MQ=255)
                                       cccATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAAt                                                    >  1:4976664/1‑93 (MQ=255)
                                         cATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATaa                                                  >  2:5003733/1‑93 (MQ=255)
                                          aTCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAg                                                 <  2:275818/93‑1 (MQ=255)
                                          aTCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAg                                                 <  1:4120909/93‑1 (MQ=255)
                                           tCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAgg                                                <  2:5893062/93‑1 (MQ=255)
                                           tCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAgg                                                <  1:1652675/93‑1 (MQ=255)
                                           tCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAgg                                                >  1:4176794/1‑93 (MQ=255)
                                            ccAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGc                                               >  2:3849871/1‑93 (MQ=255)
                                            ccAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGc                                               >  2:4450020/1‑93 (MQ=255)
                                            ccAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGc                                               <  1:1350592/93‑1 (MQ=255)
                                            ccAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGc                                               <  2:2355755/93‑1 (MQ=255)
                                              aGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGt                                             <  1:3530751/93‑1 (MQ=255)
                                               gcgcGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTc                                            <  2:4091373/93‑1 (MQ=255)
                                               gcgcGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTc                                            >  2:4167804/1‑93 (MQ=255)
                                                cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCa                                           >  1:358667/1‑93 (MQ=255)
                                                cgcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCa                                           <  1:4236577/93‑1 (MQ=255)
                                                 gcgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCAt                                          <  1:5479164/93‑1 (MQ=255)
                                                  cgGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATg                                         <  1:917196/93‑1 (MQ=255)
                                                   ggCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGc                                        >  1:4407241/1‑93 (MQ=255)
                                                    gCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCa                                       >  1:10910/1‑93 (MQ=255)
                                                    gCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCa                                       <  1:1899465/93‑1 (MQ=255)
                                                    gCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCa                                       <  2:4832824/93‑1 (MQ=255)
                                                      cGTGCATCTTTACGCCACGGACTCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAtt                                     <  2:5126823/93‑1 (MQ=255)
                                                      cGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAtt                                     <  1:6337371/93‑1 (MQ=255)
                                                       gTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAttt                                    <  2:2159248/93‑1 (MQ=255)
                                                       gTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAttt                                    <  2:5731274/93‑1 (MQ=255)
                                                        tGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAtttt                                   <  2:324143/93‑1 (MQ=255)
                                                         gCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTtg                                  <  1:2699437/93‑1 (MQ=255)
                                                          cATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTtgc                                 <  2:4687538/93‑1 (MQ=255)
                                                           aTCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTtgct                                <  1:5839477/93‑1 (MQ=255)
                                                             cTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTtgctgc                              <  2:457907/93‑1 (MQ=255)
                                                              tttACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCg                             >  1:4889054/1‑93 (MQ=255)
                                                              tttACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCg                             >  1:2606731/1‑93 (MQ=255)
                                                                tACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGaa                           >  1:1461084/1‑93 (MQ=255)
                                                                 aCGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAAc                          <  2:2697456/93‑1 (MQ=255)
                                                                   gCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGt                        <  1:1518/93‑1 (MQ=255)
                                                                   gCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGt                        >  2:5813652/1‑93 (MQ=255)
                                                                   gCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGt                        <  2:5549825/93‑1 (MQ=255)
                                                                    ccACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTc                       <  1:4483247/93‑1 (MQ=255)
                                                                    ccACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTc                       <  1:4644784/93‑1 (MQ=255)
                                                                    ccACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTc                       >  1:1247842/1‑93 (MQ=255)
                                                                    ccACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTc                       <  1:6448537/93‑1 (MQ=255)
                                                                     cACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCt                      <  1:6061231/93‑1 (MQ=255)
                                                                      aCGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTg                     <  1:2908574/93‑1 (MQ=255)
                                                                       cGGACACAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGc                    <  1:5701563/93‑1 (MQ=255)
                                                                        ggACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCa                   >  1:4558618/1‑93 (MQ=255)
                                                                          aCGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCaaa                 >  1:2957246/1‑93 (MQ=255)
                                                                               gACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGa            <  2:3799662/93‑1 (MQ=255)
                                                                               gACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGa            <  1:6232957/93‑1 (MQ=255)
                                                                                aCTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGat           >  1:4845519/1‑93 (MQ=255)
                                                                                aCTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGat           <  1:6157552/93‑1 (MQ=255)
                                                                                aCTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGat           >  2:5672024/1‑93 (MQ=255)
                                                                                  tCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGatat         >  2:4632658/1‑93 (MQ=255)
                                                                                  tCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGatat         >  2:4511333/1‑93 (MQ=255)
                                                                                   ccATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCTTGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACCGATatc        >  2:4287348/1‑93 (MQ=255)
                                                                                   ccATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatc        <  2:1530426/93‑1 (MQ=255)
                                                                                     aTTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatcat      <  2:3987122/93‑1 (MQ=255)
                                                                                      ttGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatcatc     >  1:2076119/1‑93 (MQ=255)
                                                                                      ttGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATatcatc     >  1:5299748/1‑93 (MQ=255)
                                                                                       tGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAACCGGATATCATCg    <  1:4814395/93‑1 (MQ=255)
                                                                                       tGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCg    <  1:3082869/93‑1 (MQ=255)
                                                                                         gTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAt  >  2:5974105/1‑93 (MQ=255)
                                                                                          |                                                                                           
ACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAT  >  NC_000913/3854608‑3854789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATC  >  NC_000913/3854605‑3854790
                                                                                             |                                                                                            
ACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGC                                                                                              <  SRR4302534.1203983/94‑1 (MQ=60)
   ACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAAC                                                                                           <  SRR4302534.4959851/94‑1 (MQ=60)
    CTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCCTTGGCAACT                                                                                          >  SRR4302534.1056375/1‑94 (MQ=60)
    CTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACT                                                                                          >  SRR4302534.1274322/1‑94 (MQ=60)
     TGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTG                                                                                         >  SRR4302534.250175/1‑94 (MQ=60)
     TGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTG                                                                                         >  SRR4302534.4942027/1‑94 (MQ=60)
     TGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTG                                                                                         <  SRR4302534.6378621/94‑1 (MQ=60)
      GGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGC                                                                                        <  SRR4302534.4322162/94‑1 (MQ=60)
       GCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCG                                                                                       <  SRR4302534.4789286/94‑1 (MQ=60)
       GCGAAGAGCCATCTTGTTGACGTGGACGAAACGCCCCCATCCAGCGCGCCGGTGCATCTTTACGCCACGGACGCAGACTGCATTGGCATCTGCG                                                                                       <  SRR4302534.3549582/94‑1 (MQ=60)
        CGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGG                                                                                      >  SRR4302534.1184780/1‑94 (MQ=60)
        CGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGG                                                                                      >  SRR4302534.2405638/1‑94 (MQ=60)
          AATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAA                                                                                    >  SRR4302534.389912/1‑94 (MQ=60)
           ATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGACACGGACGCAGACTCCATTGGCAACTGCGGAAC                                                                                   >  SRR4302534.1012624/1‑94 (MQ=60)
               CCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGAT                                                                               >  SRR4302534.4594410/1‑94 (MQ=60)
                CATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATC                                                                              >  SRR4302534.2805344/1‑94 (MQ=60)
                CATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATC                                                                              <  SRR4302534.3023856/94‑1 (MQ=60)
                CATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATC                                                                              >  SRR4302534.1768707/1‑94 (MQ=60)
                CATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATC                                                                              <  SRR4302534.4531343/94‑1 (MQ=60)
                 ATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCG                                                                             >  SRR4302534.4322763/1‑94 (MQ=60)
                 ATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCG                                                                             <  SRR4302534.2869760/94‑1 (MQ=60)
                 ATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCG                                                                             >  SRR4302534.6310106/1‑94 (MQ=60)
                  TCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGC                                                                            <  SRR4302534.1544587/94‑1 (MQ=60)
                   CTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACTCAGACTCCATTGGCAACTGCGGAACGGATCGCG                                                                           <  SRR4302534.2260537/94‑1 (MQ=60)
                   CTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCG                                                                           <  SRR4302534.761403/94‑1 (MQ=60)
                    TTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGA                                                                          <  SRR4302534.5921847/94‑1 (MQ=60)
                     TGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAA                                                                         <  SRR4302534.1158933/94‑1 (MQ=60)
                     TGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAA                                                                         <  SRR4302534.2990500/94‑1 (MQ=60)
                      GTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAT                                                                        >  SRR4302534.3835522/1‑94 (MQ=60)
                        GGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGTGAATTT                                                                      <  SRR4302534.4180618/94‑1 (MQ=60)
                         GACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTT                                                                     <  SRR4302534.532761/94‑1 (MQ=60)
                         GACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTT                                                                     <  SRR4302534.3376700/94‑1 (MQ=60)
                         GACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTT                                                                     <  SRR4302534.2927497/94‑1 (MQ=60)
                          ACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTG                                                                    >  SRR4302534.3957702/1‑94 (MQ=60)
                           CGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGT                                                                   <  SRR4302534.3312972/94‑1 (MQ=60)
                            GTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTC                                                                  <  SRR4302534.6331161/94‑1 (MQ=60)
                              GGACGAACCGCCCGCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCA                                                                <  SRR4302534.6612405/94‑1 (MQ=60)
                              GGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCA                                                                >  SRR4302534.2559142/1‑94 (MQ=60)
                                 CGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGT                                                             <  SRR4302534.4033125/94‑1 (MQ=60)
                                 CGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGT                                                             <  SRR4302534.1228825/94‑1 (MQ=60)
                                  GAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTTCATGTA                                                            >  SRR4302534.5796130/1‑94 (MQ=60)
                                  GAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTA                                                            >  SRR4302534.6476348/1‑94 (MQ=60)
                                  GAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAAATTTGTCCATGTA                                                            >  SRR4302534.1533005/1‑94 (MQ=60)
                                    AACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTGCATGTAAT                                                          >  SRR4302534.4251413/1‑94 (MQ=60)
                                    AACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAAT                                                          >  SRR4302534.1330420/1‑94 (MQ=60)
                                      CGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCC                                                        <  SRR4302534.4551336/94‑1 (MQ=60)
                                      CGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCC                                                        <  SRR4302534.19142/94‑1 (MQ=60)
                                      CGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCC                                                        <  SRR4302534.842692/94‑1 (MQ=60)
                                        CCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAA                                                      >  SRR4302534.3906449/1‑94 (MQ=60)
                                         CCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAAT                                                     <  SRR4302534.1971217/94‑1 (MQ=60)
                                         CCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAAT                                                     >  SRR4302534.5111377/1‑94 (MQ=60)
                                          CCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATA                                                    <  SRR4302534.2399065/94‑1 (MQ=60)
                                          CCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATA                                                    <  SRR4302534.4244958/94‑1 (MQ=60)
                                          CCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGAAACTGCTGAACGGATCGCGAATCTTGTTCATGTAATCCAATA                                                    >  SRR4302534.4952250/1‑94 (MQ=60)
                                             ATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGG                                                 <  SRR4302534.4231810/94‑1 (MQ=60)
                                             ATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGG                                                 >  SRR4302534.5410901/1‑94 (MQ=60)
                                             ATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGG                                                 >  SRR4302534.4289629/1‑94 (MQ=60)
                                             ATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGG                                                 <  SRR4302534.1592624/94‑1 (MQ=60)
                                             ATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGG                                                 >  SRR4302534.722374/1‑94 (MQ=60)
                                              TCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGC                                                <  SRR4302534.3333050/94‑1 (MQ=60)
                                              TCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGC                                                >  SRR4302534.3490952/1‑94 (MQ=60)
                                              TCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGC                                                >  SRR4302534.1594635/1‑94 (MQ=60)
                                              TCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGC                                                >  SRR4302534.5031376/1‑94 (MQ=60)
                                              TCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGC                                                <  SRR4302534.1695747/94‑1 (MQ=60)
                                               CCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCG                                               <  SRR4302534.1386193/94‑1 (MQ=60)
                                               CCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCG                                               <  SRR4302534.5894967/94‑1 (MQ=60)
                                                CAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGT                                              >  SRR4302534.4990187/1‑94 (MQ=60)
                                                 AGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTC                                             <  SRR4302534.3625720/94‑1 (MQ=60)
                                                 AGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTC                                             >  SRR4302534.2473625/1‑94 (MQ=60)
                                                 AGCGCGGCCGCGCATCTTTACGCCACGGACGCAGACTCCATTTGCAACTGCGGAACGGATCACTAATATTGTCCATGTAATCCAATAAGGCGTC                                             >  SRR4302534.195540/1‑94 (MQ=60)
                                                  GCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCA                                            >  SRR4302534.367828/1‑94 (MQ=60)
                                                  GCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCA                                            >  SRR4302534.5014082/1‑94 (MQ=60)
                                                   CGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCAT                                           <  SRR4302534.4350780/94‑1 (MQ=60)
                                                    GCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATG                                          <  SRR4302534.5314087/94‑1 (MQ=60)
                                                    GCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATG                                          <  SRR4302534.5626917/94‑1 (MQ=60)
                                                     CGGCCGTGCATCTTTACGGCACCGACGCAGGCTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGC                                         >  SRR4302534.2185221/1‑94 (MQ=60)
                                                     CGGCCGTGCATCTTTACGCCACGGACTCAGACTCCATTGGCAACTGCGGAACGGGTCGCGAATTTTGTCCCTGTAATCGAATAAagtattttgc                                         >  SRR4302534.2431831/1‑84 (MQ=60)
                                                     CGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGC                                         >  SRR4302534.4526182/1‑94 (MQ=60)
                                                     CGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGC                                         <  SRR4302534.940527/94‑1 (MQ=60)
                                                      GGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCA                                        >  SRR4302534.11184/1‑94 (MQ=60)
                                                       GCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAT                                       >  SRR4302534.1309529/1‑94 (MQ=60)
                                                       GCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCAT                                       <  SRR4302534.1949006/94‑1 (MQ=60)
                                                         CGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATTAGGCGTCATGCATTT                                     >  SRR4302534.2372529/1‑94 (MQ=60)
                                                         CGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTT                                     <  SRR4302534.6112336/94‑1 (MQ=60)
                                                         CGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTT                                     >  SRR4302534.5926366/1‑94 (MQ=60)
                                                         CGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTT                                     <  SRR4302534.6508565/94‑1 (MQ=60)
                                                         CGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTT                                     <  SRR4302534.3734580/94‑1 (MQ=60)
                                                         CGTGCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTT                                     <  SRR4302534.571173/94‑1 (MQ=60)
                                                            GCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGC                                  <  SRR4302534.2770746/94‑1 (MQ=60)
                                                            GCATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGC                                  <  SRR4302534.6431730/94‑1 (MQ=60)
                                                             CATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCT                                 <  SRR4302534.4571731/94‑1 (MQ=60)
                                                             CATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCT                                 <  SRR4302534.4629914/94‑1 (MQ=60)
                                                              ATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTG                                <  SRR4302534.5998001/94‑1 (MQ=60)
                                                              ATCTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTG                                <  SRR4302534.860649/94‑1 (MQ=60)
                                                                CTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCG                              >  SRR4302534.2675702/1‑94 (MQ=60)
                                                                CTTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCG                              >  SRR4302534.5021629/1‑94 (MQ=60)
                                                                 TTTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGA                             <  SRR4302534.5808939/94‑1 (MQ=60)
                                                                  TTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAA                            >  SRR4302534.1499503/1‑94 (MQ=60)
                                                                  TTACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAA                            <  SRR4302534.4625358/94‑1 (MQ=60)
                                                                    GCGCCACGGACGCAGACCCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACG                          <  SRR4302534.270211/94‑1 (MQ=60)
                                                                    ACGCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACG                          <  SRR4302534.4517013/94‑1 (MQ=60)
                                                                      GCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTC                        <  SRR4302534.2663687/94‑1 (MQ=60)
                                                                      GCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTC                        <  SRR4302534.1559/94‑1 (MQ=60)
                                                                      GCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTC                        >  SRR4302534.1280731/1‑94 (MQ=60)
                                                                      GCCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTC                        <  SRR4302534.3046005/94‑1 (MQ=60)
                                                                       cccaggcctcagacgcgATTGGCAACTGCGGAACGGATCGCGAATTTTGACCATGTAATCCAATAAGGCCTCATGCATTTTGCTGCGAACGTCT                       <  SRR4302534.1670707/77‑1 (MQ=60)
                                                                       CCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCT                       <  SRR4302534.6280265/94‑1 (MQ=60)
                                                                       CCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCT                       <  SRR4302534.4770862/94‑1 (MQ=60)
                                                                       CCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCT                       >  SRR4302534.4761582/1‑94 (MQ=60)
                                                                       CCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCT                       <  SRR4302534.4604539/94‑1 (MQ=60)
                                                                       CCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCT                       <  SRR4302534.6622324/94‑1 (MQ=60)
                                                                       CCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCT                       >  SRR4302534.4051042/1‑94 (MQ=60)
                                                                       CCACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCT                       <  SRR4302534.1752417/94‑1 (MQ=60)
                                                                        CACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTG                      <  SRR4302534.6226044/94‑1 (MQ=60)
                                                                        CACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTG                      >  SRR4302534.727062/1‑94 (MQ=60)
                                                                        CACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTG                      <  SRR4302534.5691304/94‑1 (MQ=60)
                                                                        CACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTG                      >  SRR4302534.2520651/1‑94 (MQ=60)
                                                                        CACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTG                      >  SRR4302534.5543043/1‑94 (MQ=60)
                                                                        CACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTG                      <  SRR4302534.5490013/94‑1 (MQ=60)
                                                                        CACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTG                      <  SRR4302534.2976997/94‑1 (MQ=60)
                                                                         ACGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGC                     <  SRR4302534.2984922/94‑1 (MQ=60)
                                                                          CGGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCA                    >  SRR4302534.4682212/1‑94 (MQ=60)
                                                                          CGGACACAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCA                    <  SRR4302534.5856005/94‑1 (MQ=60)
                                                                           GGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAA                   <  SRR4302534.5392968/94‑1 (MQ=60)
                                                                           GGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAA                   <  SRR4302534.5799699/94‑1 (MQ=60)
                                                                           GGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAA                   >  SRR4302534.3470712/1‑94 (MQ=60)
                                                                           GGACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATACAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAA                   >  SRR4302534.2979735/1‑94 (MQ=60)
                                                                            GACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCTATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAA                  <  SRR4302534.1832008/94‑1 (MQ=60)
                                                                            GACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAA                  >  SRR4302534.6601122/1‑94 (MQ=60)
                                                                            GACGCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAA                  >  SRR4302534.3034749/1‑94 (MQ=60)
                                                                               GCAGACTCCATTGGCAACTGCGTAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACG               <  SRR4302534.3135730/94‑1 (MQ=60)
                                                                               GCAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACG               >  SRR4302534.434511/1‑94 (MQ=60)
                                                                                CAGACTCCATTGGCAACTGCGGAACGGATCGCGGATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGGACGTCTGCAAAACGG              >  SRR4302534.6214189/1‑94 (MQ=60)
                                                                                CAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGG              <  SRR4302534.2640266/94‑1 (MQ=60)
                                                                                CAGACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGG              >  SRR4302534.1238414/1‑94 (MQ=60)
                                                                                  GACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGAT            >  SRR4302534.4976981/1‑94 (MQ=60)
                                                                                  GACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGAT            <  SRR4302534.3629501/94‑1 (MQ=60)
                                                                                  GACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGAT            >  SRR4302534.1434526/1‑94 (MQ=60)
                                                                                  GACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGAT            <  SRR4302534.6402140/94‑1 (MQ=60)
                                                                                   ACTCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATA           <  SRR4302534.6325038/94‑1 (MQ=60)
                                                                                   ACTCCATCGGCACCTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATA           <  SRR4302534.2525896/94‑1 (MQ=60)
                                                                                     TCCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATC         >  SRR4302534.4741641/1‑94 (MQ=60)
                                                                                      CCATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCA        >  SRR4302534.204550/1‑94 (MQ=60)
                                                                                       CATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCAT       <  SRR4302534.1444872/94‑1 (MQ=60)
                                                                                       CATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCAT       <  SRR4302534.5118841/94‑1 (MQ=60)
                                                                                        ATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATC      >  SRR4302534.5442763/1‑94 (MQ=60)
                                                                                        ATTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATC      >  SRR4302534.2130471/1‑94 (MQ=60)
                                                                                         TTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCG     <  SRR4302534.1062863/94‑1 (MQ=60)
                                                                                         TTGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCG     <  SRR4302534.5861858/94‑1 (MQ=60)
                                                                                          TGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAACCGGATATCATCGA    <  SRR4302534.4945084/94‑1 (MQ=60)
                                                                                          TGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGA    >  SRR4302534.3203989/1‑94 (MQ=60)
                                                                                          TGGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGA    <  SRR4302534.3163866/94‑1 (MQ=60)
                                                                                           GGCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGAT   <  SRR4302534.3709248/94‑1 (MQ=60)
                                                                                            GCAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATC  >  SRR4302534.6300830/1‑94 (MQ=60)
                                                                                             |                                                                                            
ACAACTGGCGAATAGCCATCTTGTGGACGTGGACGAAACGCCCCCATCCAGCGCGGCCGTGCATCTTTACGCCACGGACGCAGACTCCATTGGTAACTGCGGAACGGATCGCGAATTTTGTCCATGTAATCCAATAAGGCGTCATGCATTTTGCTGCGAACGTCTGCAAAACGGATATCATCGATC  >  NC_000913/3854605‑3854790

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑