Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,872,450 +G coding (338/387 nt) yidW ← regulator protein for dgo operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,872,4501.G99.2% 1222.2 / 1.2 356coding (338/387 nt)yidWregulator protein for dgo operon
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base G (196/157);  total (197/159)
Fisher's exact test for biased strand distribution p-value = 5.88e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.32e-01

Only 100 of 394 total aligned reads displayed.

TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑CCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536
                                                                                              |                                                                                       
 cGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑tg                                                                                         >  2:3793811/1‑92 (MQ=255)
  gTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑tgc                                                                                        >  2:7982217/1‑91 (MQ=255)
  gTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑tgc                                                                                        <  2:8372968/93‑3 (MQ=255)
    tAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑gccc                                                                                     <  2:3391244/93‑2 (MQ=255)
     aaaCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑gccca                                                                                    >  1:6161923/1‑91 (MQ=255)
      aaCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑GCCC‑Ac                                                                                  >  2:2490733/1‑93 (MQ=255)
       aCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑gcccgac                                                                                  <  1:6870690/93‑5 (MQ=255)
        cGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACg                                                                                 <  1:7060968/93‑1 (MQ=255)
         gCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑AAgg                                                                                >  1:6499437/1‑93 (MQ=255)
           ttCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGc                                                                              >  2:2848888/1‑93 (MQ=255)
           ttCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGc                                                                              >  1:8286268/1‑93 (MQ=255)
            tCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCa                                                                             >  2:2903307/1‑93 (MQ=255)
            tCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGAGCTCTGC‑TGCCCA‑ACGGGCa                                                                             >  2:8056614/1‑93 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGc                                                                        >  1:6374156/1‑93 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGc                                                                        >  2:8430691/1‑93 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGc                                                                        <  1:2499356/93‑1 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGc                                                                        <  2:6150995/93‑1 (MQ=255)
                  tCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGcc                                                                       >  2:8489720/1‑93 (MQ=255)
                     tGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGt                                                                    <  2:5048605/93‑1 (MQ=255)
                        ttgttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTcc                                                                 >  1:1087739/1‑93 (MQ=255)
                         tgttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTcca                                                                <  1:5990898/93‑1 (MQ=255)
                              gCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGa                                                           >  1:7580231/1‑93 (MQ=255)
                               cAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAt                                                          <  1:4378862/93‑1 (MQ=255)
                               cAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAt                                                          <  2:7119166/93‑1 (MQ=255)
                                aaTCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAtt                                                         >  1:6569463/1‑93 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAttt                                                        >  1:8639804/1‑93 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAttt                                                        <  1:4546610/93‑1 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAttt                                                        >  2:2016493/1‑93 (MQ=255)
                                 accatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGAttt                                                        >  1:8424834/3‑93 (MQ=255)
                                      ctcGTTCAGCGCCGATTCACTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑CCGGGCAATCGCCGGTTCCACCCGATTTCGCAc                                                   <  1:7837754/93‑1 (MQ=255)
                                      ctcGTTCAGCGCCGATTAAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCAc                                                   <  2:8803472/93‑1 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  1:3091302/1‑93 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  <  1:8810372/93‑1 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  2:1050293/1‑93 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  <  1:7750922/93‑1 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  2:1487362/1‑93 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  1:3687740/1‑93 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  >  2:4349448/1‑93 (MQ=255)
                                        cGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACtt                                                 <  1:6965913/93‑1 (MQ=255)
                                        cGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACtt                                                 <  1:5159303/93‑1 (MQ=255)
                                         gtacAGCGCCTAGTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTc                                                <  2:4602088/90‑1 (MQ=255)
                                         gTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTc                                                >  2:3637274/1‑93 (MQ=255)
                                         gTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTc                                                >  2:6174673/1‑93 (MQ=255)
                                          ttCAGCGCCGATTCAATCTGCGCCAGATCGCTGTAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGCTCCACCAGATTTCGCACTTCg                                               >  1:1788407/1‑93 (MQ=255)
                                          ttCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCg                                               >  1:5062773/1‑93 (MQ=255)
                                          ttCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCg                                               >  2:8116443/1‑93 (MQ=255)
                                           tCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGc                                              <  2:6691719/93‑1 (MQ=255)
                                           tCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGc                                              >  1:1296175/1‑93 (MQ=255)
                                             aGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTc                                            >  1:2677545/1‑93 (MQ=255)
                                               cgcCGATTCAATCTGCGCCAGATCGCTAGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCAt                                          <  1:3954110/93‑1 (MQ=255)
                                                gcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATg                                         <  2:4678368/93‑1 (MQ=255)
                                                  cGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGc                                       <  1:752838/93‑1 (MQ=255)
                                                   gATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      >  1:7862486/1‑93 (MQ=255)
                                                   gATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      >  1:5268295/1‑93 (MQ=255)
                                                   gATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCa                                      >  2:3069818/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     >  2:5682012/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     >  1:8706758/1‑93 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     <  2:3240993/93‑1 (MQ=255)
                                                    aTTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAc                                     <  2:1271115/93‑1 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    <  2:2405801/93‑1 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    >  1:4856640/1‑93 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    >  2:3868805/1‑93 (MQ=255)
                                                      tCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTg                                   <  1:6779664/93‑1 (MQ=255)
                                                      tCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCACTGCAG‑CCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCACTCATGGCACTg                                   <  2:7834932/93‑1 (MQ=255)
                                                       cAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGa                                  <  2:6530669/93‑1 (MQ=255)
                                                       cAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGa                                  <  1:4810601/93‑1 (MQ=255)
                                                         agcTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATa                                <  2:7679884/91‑1 (MQ=255)
                                                            tGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             <  2:6929155/93‑1 (MQ=255)
                                                            tGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             <  2:1621574/93‑1 (MQ=255)
                                                            cgcgcCAGCTCGCTGGAAGTCGCGCACTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGc                             <  1:192717/92‑1 (MQ=255)
                                                             gcgcCAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGcc                            <  1:53212/93‑1 (MQ=255)
                                                                ccAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTg                         <  2:2739179/93‑1 (MQ=255)
                                                                ccAGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTg                         >  2:592464/1‑93 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       <  2:6322399/93‑1 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       <  2:2166437/93‑1 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       >  2:4981641/1‑93 (MQ=255)
                                                                    aTCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTc                     >  2:3984281/1‑93 (MQ=255)
                                                                    aTCGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTc                     >  1:7304883/1‑93 (MQ=255)
                                                                      cGCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGt                   >  1:1180485/1‑93 (MQ=255)
                                                                       gCTGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTa                  >  1:2931941/1‑93 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                <  2:1719201/93‑1 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                <  2:8730377/93‑1 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                >  1:3896733/1‑93 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                >  1:4085380/1‑93 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGC‑TGCACA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                <  2:5271049/93‑1 (MQ=255)
                                                                             attCGCACTCTCTGC‑T‑ACGATACGGGCAATCGCCGGTTCCACCAGATTTCGCTCTTCGCTCATGGCACTGATAAGCCGTGGGTAGTAGTCAtt            <  2:4906222/91‑1 (MQ=255)
                                                                             aGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            >  2:5763623/1‑93 (MQ=255)
                                                                             aGTCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            <  1:4629903/93‑1 (MQ=255)
                                                                               tCGCGCGCTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtttt          <  2:1156392/93‑1 (MQ=255)
                                                                                 gcgcgcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTcc        >  1:1659352/1‑93 (MQ=255)
                                                                                   gcgcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAg      >  2:5562537/1‑93 (MQ=255)
                                                                                   gcgcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAg      >  2:7293208/1‑93 (MQ=255)
                                                                                    cgcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGc     >  1:5475503/1‑93 (MQ=255)
                                                                                    cgcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGc     <  2:19089/93‑1 (MQ=255)
                                                                                     gcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCa    >  2:7533176/1‑93 (MQ=255)
                                                                                     gcTCTGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCa    >  1:3315371/1‑93 (MQ=255)
                                                                                     cctctGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCa    <  1:8095393/92‑1 (MQ=255)
                                                                                      ctctGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAc   >  1:3498073/1‑93 (MQ=255)
                                                                                       tctGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAcc  >  1:4235184/1‑93 (MQ=255)
                                                                                       tctGC‑TGCCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAcc  <  1:740093/93‑1 (MQ=255)
                                                                                              |                                                                                       
TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGC‑T‑CCCA‑ACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 201 total aligned reads displayed.
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCAT  >  NC_000913/3872357‑3872539
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TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT                                                                                            >  SRR4302541.7485430/1‑94 (MQ=60)
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT                                                                                            <  SRR4302541.6909903/94‑1 (MQ=60)
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT                                                                                            <  SRR4302541.1168695/94‑1 (MQ=60)
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT                                                                                            <  SRR4302541.8262793/94‑1 (MQ=60)
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT                                                                                            <  SRR4302541.6974057/94‑1 (MQ=60)
 TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑G                                                                                          <  SRR4302541.5191297/94‑1 (MQ=60)
 TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑G                                                                                          >  SRR4302541.4361015/1‑94 (MQ=60)
  CGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑GC                                                                                         >  SRR4302541.5712193/1‑94 (MQ=60)
  CGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑GC                                                                                         >  SRR4302541.3341506/1‑94 (MQ=60)
  CGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑GC                                                                                         >  SRR4302541.7458496/1‑94 (MQ=60)
     TAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCA                                                                                       <  SRR4302541.657302/94‑1 (MQ=60)
     TAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCA                                                                                       >  SRR4302541.6224893/1‑94 (MQ=60)
     TAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCA                                                                                       <  SRR4302541.7653065/94‑1 (MQ=60)
     TAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑GCCAA                                                                                      <  SRR4302541.531663/94‑1 (MQ=60)
      TAACGCTCCGCGGTCCTGATTGATGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAA                                                                                      <  SRR4302541.2980839/94‑1 (MQ=60)
        ACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTgcccgacg                                                                                    <  SRR4302541.6944077/94‑9 (MQ=60)
        ACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACG                                                                                    <  SRR4302541.4692124/94‑1 (MQ=60)
        ACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACG                                                                                    <  SRR4302541.7840774/94‑1 (MQ=60)
         CGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGG                                                                                   <  SRR4302541.7136061/94‑1 (MQ=60)
         CGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAAAGG                                                                                   >  SRR4302541.6565715/1‑94 (MQ=60)
          GCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGG                                                                                  >  SRR4302541.6836194/1‑94 (MQ=60)
           CTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGTC                                                                                 >  SRR4302541.6912707/1‑94 (MQ=60)
           CTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGC                                                                                 >  SRR4302541.3296249/1‑94 (MQ=60)
           CTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGC                                                                                 >  SRR4302541.8376389/1‑94 (MQ=60)
           CTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGC                                                                                 <  SRR4302541.8737273/94‑1 (MQ=60)
           CTGCGCGGTCCTGATTGTGGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGC                                                                                 <  SRR4302541.8737274/94‑1 (MQ=60)
            TTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCA                                                                                >  SRR4302541.870672/1‑94 (MQ=60)
              CGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAAT                                                                              <  SRR4302541.4018895/94‑1 (MQ=60)
               GCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATC                                                                             <  SRR4302541.5530662/94‑1 (MQ=60)
               GCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATC                                                                             <  SRR4302541.4342687/94‑1 (MQ=60)
                CGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATTGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCG                                                                            >  SRR4302541.2665886/1‑94 (MQ=60)
                CGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCG                                                                            >  SRR4302541.6965369/1‑94 (MQ=60)
                CGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCG                                                                            <  SRR4302541.8127637/94‑1 (MQ=60)
                 GGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATGGC                                                                           >  SRR4302541.7851399/1‑94 (MQ=60)
                 GGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGC                                                                           >  SRR4302541.7601510/1‑94 (MQ=60)
                 GGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGC                                                                           >  SRR4302541.6439289/1‑94 (MQ=60)
                 GGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGC                                                                           >  SRR4302541.660339/1‑94 (MQ=60)
                 GGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGC                                                                           >  SRR4302541.793065/1‑94 (MQ=60)
                 GGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGCGCAATCGC                                                                           >  SRR4302541.3175557/1‑94 (MQ=60)
                  GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCC                                                                          >  SRR4302541.4957133/1‑94 (MQ=60)
                  GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCC                                                                          <  SRR4302541.2524580/94‑1 (MQ=60)
                  GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCC                                                                          >  SRR4302541.4643592/1‑94 (MQ=60)
                  GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCC                                                                          <  SRR4302541.7827081/94‑1 (MQ=60)
                  GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCC                                                                          >  SRR4302541.7818166/1‑94 (MQ=60)
                  GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCC                                                                          <  SRR4302541.7040618/94‑1 (MQ=60)
                   ttctgattgtgggaaatcgttgCGTTCAGCCCCGATTCATTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCG                                                                         <  SRR4302541.5340997/72‑1 (MQ=60)
                   TCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCACTCGCCG                                                                         >  SRR4302541.8843044/1‑94 (MQ=60)
                   TCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCG                                                                         <  SRR4302541.5340996/94‑1 (MQ=60)
                    CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGG                                                                        >  SRR4302541.4121348/1‑94 (MQ=60)
                     CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGT                                                                       >  SRR4302541.6623167/1‑94 (MQ=60)
                     CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGT                                                                       >  SRR4302541.8784885/1‑94 (MQ=60)
                      TGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTT                                                                      >  SRR4302541.3377332/1‑94 (MQ=60)
                       GATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTC                                                                     >  SRR4302541.7119138/1‑94 (MQ=60)
                       GATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTC                                                                     <  SRR4302541.8619278/94‑1 (MQ=60)
                       GATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTAGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTC                                                                     >  SRR4302541.6068161/1‑94 (MQ=60)
                        ATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCC                                                                    >  SRR4302541.1097903/1‑94 (MQ=60)
                          TGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCAC                                                                  <  SRR4302541.6052263/94‑1 (MQ=60)
                           GTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACC                                                                 >  SRR4302541.2763168/1‑94 (MQ=60)
                             TGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAG                                                               >  SRR4302541.7415900/1‑94 (MQ=60)
                             TGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCCCTCTGCTGCCCCACGGACAATCGCCGGTTCCACCAA                                                               >  SRR4302541.7415898/1‑94 (MQ=60)
                              GGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGA                                                              >  SRR4302541.7661216/1‑94 (MQ=60)
                               GCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCAGGTTCCACCAGAT                                                             >  SRR4302541.2763210/1‑94 (MQ=60)
                                CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                            >  SRR4302541.3719467/1‑94 (MQ=60)
                                CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                            <  SRR4302541.4888647/94‑1 (MQ=60)
                                CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                            <  SRR4302541.4422622/94‑1 (MQ=60)
                                CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                            >  SRR4302541.6636671/1‑94 (MQ=60)
                                 AATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTT                                                           >  SRR4302541.8732353/1‑94 (MQ=60)
                                 AATCATCCCCTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTT                                                           <  SRR4302541.7575191/94‑1 (MQ=60)
                                 AACCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTT                                                           >  SRR4302541.8515889/1‑94 (MQ=60)
                                  ATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTC                                                          <  SRR4302541.4592600/94‑1 (MQ=60)
                                    CATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGC                                                        >  SRR4302541.1913370/1‑94 (MQ=60)
                                    CATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGC                                                        >  SRR4302541.8504310/1‑94 (MQ=60)
                                    CATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGC                                                        <  SRR4302541.5249251/94‑1 (MQ=60)
                                    CATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGC                                                        >  SRR4302541.5178826/1‑94 (MQ=60)
                                     ATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGAGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGctttacca                                                       >  SRR4302541.1318748/1‑86 (MQ=60)
                                      TCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAC                                                      <  SRR4302541.4501746/94‑1 (MQ=60)
                                       CTCGTTCAGCGCCGATTCACTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCACCGGGCAATCGCCGGTTCCACCCGATTTCGCACT                                                     <  SRR4302541.7923326/94‑1 (MQ=60)
                                       CTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACT                                                     <  SRR4302541.7297313/94‑1 (MQ=60)
                                       CTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACT                                                     >  SRR4302541.3122655/1‑94 (MQ=60)
                                       CTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACT                                                     >  SRR4302541.3725061/1‑94 (MQ=60)
                                        TCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTT                                                    <  SRR4302541.7833388/94‑1 (MQ=60)
                                        TCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTT                                                    <  SRR4302541.5448182/94‑1 (MQ=60)
                                        TCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTT                                                    <  SRR4302541.8904451/94‑1 (MQ=60)
                                         CGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTC                                                   <  SRR4302541.7040161/94‑1 (MQ=60)
                                         CGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTC                                                   <  SRR4302541.5212378/94‑1 (MQ=60)
                                         CGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTC                                                   <  SRR4302541.3349086/94‑1 (MQ=60)
                                         CGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTC                                                   <  SRR4302541.831079/94‑1 (MQ=60)
                                         CGTGCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTC                                                   <  SRR4302541.8379391/94‑1 (MQ=60)
                                          GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGTAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGCTCCACCAGATTTCGCACTTCG                                                  >  SRR4302541.1805782/1‑94 (MQ=60)
                                          GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                  >  SRR4302541.3076130/1‑94 (MQ=60)
                                          GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                  >  SRR4302541.5114832/1‑94 (MQ=60)
                                          GTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                  >  SRR4302541.643456/1‑94 (MQ=60)
                                          CTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCG                                                  <  SRR4302541.1849280/94‑1 (MQ=60)
                                           TTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGC                                                 >  SRR4302541.1308965/1‑94 (MQ=60)
                                           TTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGC                                                 >  SRR4302541.2247038/1‑94 (MQ=60)
                                            TCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCT                                                <  SRR4302541.7156445/94‑1 (MQ=60)
                                             tcGCGCCGATTCCATCTGCGCCAGATCGCTGGAAGTCGCCCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTC                                               <  SRR4302541.2318537/92‑1 (MQ=60)
                                             CAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTC                                               <  SRR4302541.4895048/94‑1 (MQ=60)
                                             CAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTC                                               >  SRR4302541.2704144/1‑94 (MQ=60)
                                              AGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCA                                              >  SRR4302541.6979885/1‑94 (MQ=60)
                                              AGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCA                                              >  SRR4302541.4645195/1‑94 (MQ=60)
                                              AGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCA                                              >  SRR4302541.5367610/1‑94 (MQ=60)
                                                CGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                            >  SRR4302541.1652317/1‑94 (MQ=60)
                                                CGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                            <  SRR4302541.4151132/94‑1 (MQ=60)
                                                CGCCGATTCAATCTGCGCCAGATCGCTAGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                            <  SRR4302541.3993417/94‑1 (MQ=60)
                                                 GCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                           >  SRR4302541.676933/1‑94 (MQ=60)
                                                 GCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                           <  SRR4302541.7936282/94‑1 (MQ=60)
                                                 GACGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                           <  SRR4302541.6467979/94‑1 (MQ=60)
                                                  CCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGC                                          >  SRR4302541.6885466/1‑94 (MQ=60)
                                                   CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                         >  SRR4302541.5322575/1‑94 (MQ=60)
                                                   CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                         >  SRR4302541.7948849/1‑94 (MQ=60)
                                                   CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                         >  SRR4302541.7948846/1‑94 (MQ=60)
                                                   CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                         <  SRR4302541.759941/94‑1 (MQ=60)
                                                   CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                         >  SRR4302541.3964829/1‑94 (MQ=60)
                                                   CGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCA                                         >  SRR4302541.3965006/1‑94 (MQ=60)
                                                    GATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAC                                        >  SRR4302541.8799909/1‑94 (MQ=60)
                                                    GATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCAC                                        >  SRR4302541.8900846/1‑94 (MQ=60)
                                                     ATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACT                                       >  SRR4302541.933289/1‑94 (MQ=60)
                                                     ATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACT                                       <  SRR4302541.4552270/94‑1 (MQ=60)
                                                     ATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACT                                       >  SRR4302541.4906163/1‑94 (MQ=60)
                                                      TTCATTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTG                                      >  SRR4302541.8268577/1‑94 (MQ=60)
                                                      TTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTG                                      <  SRR4302541.3986076/94‑1 (MQ=60)
                                                       TCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGA                                     >  SRR4302541.6274733/1‑94 (MQ=60)
                                                       TCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGA                                     <  SRR4302541.6852170/94‑1 (MQ=60)
                                                        CAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAT                                    <  SRR4302541.5835454/94‑1 (MQ=60)
                                                        CAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAT                                    <  SRR4302541.1853136/94‑1 (MQ=60)
                                                        CAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAT                                    <  SRR4302541.4220675/94‑1 (MQ=60)
                                                        CAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAT                                    <  SRR4302541.4859654/94‑1 (MQ=60)
                                                         AATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATA                                   <  SRR4302541.133797/94‑1 (MQ=60)
                                                         AATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATA                                   <  SRR4302541.1222759/94‑1 (MQ=60)
                                                          ATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCCCTTCGCTCATGGCACTGATAA                                  <  SRR4302541.5209014/94‑1 (MQ=60)
                                                          ATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAA                                  <  SRR4302541.6682727/94‑1 (MQ=60)
                                                           TCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAG                                 >  SRR4302541.6239861/1‑94 (MQ=60)
                                                            CTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGC                                <  SRR4302541.8092205/94‑1 (MQ=60)
                                                            CTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGC                                <  SRR4302541.7796248/94‑1 (MQ=60)
                                                            CTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGC                                <  SRR4302541.1118985/94‑1 (MQ=60)
                                                             TGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                               >  SRR4302541.8193188/1‑94 (MQ=60)
                                                             TGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                               <  SRR4302541.8083376/94‑1 (MQ=60)
                                                             CGCGCCAGCTCGCTGGAAGTCGCGCACTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                               <  SRR4302541.194181/94‑1 (MQ=60)
                                                              GCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCG                              <  SRR4302541.8602980/94‑1 (MQ=60)
                                                              GCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCG                              <  SRR4302541.53630/94‑1 (MQ=60)
                                                              GCGCCAGATCACTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCG                              <  SRR4302541.6149169/94‑1 (MQ=60)
                                                               CGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGG                             >  SRR4302541.3717794/1‑94 (MQ=60)
                                                                GCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTG                            <  SRR4302541.3866169/94‑1 (MQ=60)
                                                                 CCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGG                           <  SRR4302541.4017669/94‑1 (MQ=60)
                                                                 CCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGG                           <  SRR4302541.4770140/94‑1 (MQ=60)
                                                                   AGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                         >  SRR4302541.3750364/1‑94 (MQ=60)
                                                                   AGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                         <  SRR4302541.5468652/94‑1 (MQ=60)
                                                                   AGATCGCTGGAAGGCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                         <  SRR4302541.7642430/94‑1 (MQ=60)
                                                                    GATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                        >  SRR4302541.7071589/1‑94 (MQ=60)
                                                                    GATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                        >  SRR4302541.7382863/1‑94 (MQ=60)
                                                                    GATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                        <  SRR4302541.6540170/94‑1 (MQ=60)
                                                                     ATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCG                       >  SRR4302541.1802876/1‑94 (MQ=60)
                                                                     ATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCG                       <  SRR4302541.4613778/94‑1 (MQ=60)
                                                                      TCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGT                      >  SRR4302541.1191897/1‑94 (MQ=60)
                                                                      TCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGT                      >  SRR4302541.4574464/1‑94 (MQ=60)
                                                                       CGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTA                     <  SRR4302541.8841734/94‑1 (MQ=60)
                                                                       CGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTA                     >  SRR4302541.2961421/1‑94 (MQ=60)
                                                                       CGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTA                     >  SRR4302541.3895233/1‑94 (MQ=60)
                                                                       CGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTA                     >  SRR4302541.5225267/1‑94 (MQ=60)
                                                                        GCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAG                    >  SRR4302541.4956185/1‑94 (MQ=60)
                                                                        GCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAG                    <  SRR4302541.2476722/94‑1 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGT                   <  SRR4302541.1214058/94‑1 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGT                   >  SRR4302541.3935751/1‑94 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGT                   >  SRR4302541.4125700/1‑94 (MQ=60)
                                                                          TGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTC                  >  SRR4302541.7613519/1‑94 (MQ=60)
                                                                          TGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTC                  <  SRR4302541.2941448/94‑1 (MQ=60)
                                                                          TGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTC                  <  SRR4302541.1786359/94‑1 (MQ=60)
                                                                          TGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTT‑CACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCTTAGTCA                 >  SRR4302541.586986/1‑94 (MQ=60)
                                                                            GAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAT                >  SRR4302541.4188048/1‑94 (MQ=60)
                                                                             AAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATT               <  SRR4302541.1715658/94‑1 (MQ=60)
                                                                             AAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACCGATAAGCCGTGGGTCGTAGTCATT               >  SRR4302541.1294261/1‑94 (MQ=60)
                                                                              AGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTT              <  SRR4302541.4677012/94‑1 (MQ=60)
                                                                              AGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTT              >  SRR4302541.6933396/1‑94 (MQ=60)
                                                                               GTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTT             <  SRR4302541.3717306/94‑1 (MQ=60)
                                                                                TCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTC            <  SRR4302541.6196526/94‑1 (MQ=60)
                                                                                 CGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCC           >  SRR4302541.1675723/1‑94 (MQ=60)
                                                                                 CGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCC           >  SRR4302541.7232922/1‑94 (MQ=60)
                                                                                 CGAGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCC           >  SRR4302541.6370988/1‑94 (MQ=60)
                                                                                  GCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCA          <  SRR4302541.1567518/94‑1 (MQ=60)
                                                                                   CGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAG         >  SRR4302541.8389094/1‑94 (MQ=60)
                                                                                   CCCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAG         <  SRR4302541.5105548/94‑1 (MQ=60)
                                                                                    GCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGC        >  SRR4302541.5531646/1‑94 (MQ=60)
                                                                                    GCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGC        <  SRR4302541.5351933/94‑1 (MQ=60)
                                                                                    ACGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGC        <  SRR4302541.110483/94‑1 (MQ=60)
                                                                                     CGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGTACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCA       >  SRR4302541.303835/1‑94 (MQ=60)
                                                                                     CGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCA       <  SRR4302541.2696690/94‑1 (MQ=60)
                                                                                     CGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCA       >  SRR4302541.3348642/1‑94 (MQ=60)
                                                                                     CGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCA       >  SRR4302541.4823392/1‑94 (MQ=60)
                                                                                      GCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAC      >  SRR4302541.3533160/1‑94 (MQ=60)
                                                                                      GCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAC      >  SRR4302541.3522692/1‑94 (MQ=60)
                                                                                      CCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAC      <  SRR4302541.8184141/94‑1 (MQ=60)
                                                                                       CTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC     <  SRR4302541.2562088/94‑1 (MQ=60)
                                                                                       CTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC     >  SRR4302541.4277405/1‑94 (MQ=60)
                                                                                       CTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC     >  SRR4302541.4882214/1‑94 (MQ=60)
                                                                                        TCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCC    <  SRR4302541.747124/94‑1 (MQ=60)
                                                                                        TCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCC    >  SRR4302541.1746986/1‑94 (MQ=60)
                                                                                         CTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGACGTGGGTCGTAGTCATTTTACAGCACCCA   >  SRR4302541.3138415/1‑94 (MQ=60)
                                                                                          TGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCAT  >  SRR4302541.8792372/1‑94 (MQ=60)
                                                                                          TGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCAT  <  SRR4302541.2563088/94‑1 (MQ=60)
                                                                                             |                                                                                          
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCAT  >  NC_000913/3872357‑3872539

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑