Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,872,450 +G coding (338/387 nt) yidW ← regulator protein for dgo operon

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,872,4501.G100.0% 935.6 / NA 266E113A (GAG→GCG) yidWregulator protein for dgo operon
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (133/133);  total (133/133)

Only 100 of 288 total aligned reads displayed.

TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536
                                                                                             |                                                                                      
tCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCt                                                                                         <  2:5487285/93‑1 (MQ=255)
tCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCt                                                                                         >  1:2365919/1‑93 (MQ=255)
   ttAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑gcc                                                                                     >  1:2851511/1‑93 (MQ=255)
    tAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑gccc                                                                                    <  1:3830275/93‑2 (MQ=255)
      aaCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCaa                                                                                   >  1:6330714/1‑93 (MQ=255)
       aCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAAc                                                                                  <  2:3527961/93‑1 (MQ=255)
       aCGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAAc                                                                                  >  1:5618886/1‑93 (MQ=255)
        cGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACg                                                                                 <  2:5162409/93‑1 (MQ=255)
        cGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACg                                                                                 <  2:4780288/93‑1 (MQ=255)
        cGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACg                                                                                 <  2:4257051/93‑1 (MQ=255)
        cGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACg                                                                                 >  1:3566923/1‑93 (MQ=255)
         gCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACgg                                                                                >  1:673561/1‑93 (MQ=255)
         gCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACgg                                                                                >  2:1228395/1‑93 (MQ=255)
           ttCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGc                                                                              >  1:2307521/1‑93 (MQ=255)
            tCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCa                                                                             <  2:4597193/93‑1 (MQ=255)
            tCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCa                                                                             >  1:2918312/1‑93 (MQ=255)
             cgcgGTCCTGATTGTTGGCAATCAGCTCGTTCAGCTCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGATCTGCTGCCCAACGGGCaa                                                                            <  2:6432064/93‑1 (MQ=255)
              gcgGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAAt                                                                           <  2:625840/93‑1 (MQ=255)
              gcgGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAAt                                                                           <  2:5581011/93‑1 (MQ=255)
                ggTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCg                                                                         >  2:1943256/1‑93 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGc                                                                        <  2:2653696/93‑1 (MQ=255)
                 gTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGc                                                                        >  1:6120103/1‑93 (MQ=255)
                  tCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGcc                                                                       <  2:4130754/93‑1 (MQ=255)
                   ccTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCg                                                                      <  2:5981896/93‑1 (MQ=255)
                    cTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCgg                                                                     >  2:3923320/1‑93 (MQ=255)
                    cTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCgg                                                                     >  1:4253499/1‑93 (MQ=255)
                      gATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCCATCGCCGGtc                                                                   >  1:5801377/1‑92 (MQ=255)
                       aTTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTc                                                                  >  2:1174091/1‑93 (MQ=255)
                        ttgttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTcc                                                                 <  2:4030100/93‑1 (MQ=255)
                          gttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTccac                                                               >  1:6260615/1‑93 (MQ=255)
                           ttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTccacc                                                              >  2:5781445/1‑93 (MQ=255)
                           ttgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTccacc                                                              <  1:5122369/93‑1 (MQ=255)
                            tgGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTccacca                                                             <  1:5354154/93‑1 (MQ=255)
                              gCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGa                                                           <  1:4898360/93‑1 (MQ=255)
                               cAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGAt                                                          <  2:3084323/93‑1 (MQ=255)
                               cAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGAt                                                          <  1:5285303/93‑1 (MQ=255)
                                aaTCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGAtt                                                         >  2:5538489/1‑93 (MQ=255)
                                aaTCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGAtt                                                         <  1:456327/93‑1 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGAttt                                                        >  2:3642693/1‑93 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGAttt                                                        >  1:1576698/1‑93 (MQ=255)
                                 atcatcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGAttt                                                        >  2:6359804/1‑93 (MQ=255)
                                    atcTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGc                                                     >  2:4187745/1‑93 (MQ=255)
                                     tctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCa                                                    >  1:4502775/1‑93 (MQ=255)
                                      ctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAc                                                   <  1:4635134/93‑1 (MQ=255)
                                      ctcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAc                                                   >  1:6419143/1‑93 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  <  1:1674009/93‑1 (MQ=255)
                                       tcGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACt                                                  <  1:1799901/93‑1 (MQ=255)
                                        cGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACtt                                                 <  2:378466/93‑1 (MQ=255)
                                        cGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACtt                                                 >  1:2697966/1‑93 (MQ=255)
                                         gTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTc                                                <  2:4323674/93‑1 (MQ=255)
                                         gTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTc                                                <  2:1318481/93‑1 (MQ=255)
                                            cAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCt                                             >  1:2490852/1‑93 (MQ=255)
                                             aGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTc                                            <  1:3720404/93‑1 (MQ=255)
                                              gcgcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCa                                           <  2:2081580/93‑1 (MQ=255)
                                                gcCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATg                                         >  1:1960800/1‑93 (MQ=255)
                                                 ccGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACTAGATTTCGCACTTCGCTCATgg                                        >  2:6111650/1‑93 (MQ=255)
                                                 ccGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATgg                                        <  1:2941849/93‑1 (MQ=255)
                                                 ccGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATgg                                        >  1:5955396/1‑93 (MQ=255)
                                                  cGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGc                                       <  2:1047506/93‑1 (MQ=255)
                                                     ttCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACt                                    <  2:5380769/93‑1 (MQ=255)
                                                       cAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGa                                  <  2:1830819/93‑1 (MQ=255)
                                                         aTCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATa                                <  1:4989479/93‑1 (MQ=255)
                                                          tCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATaa                               <  2:1743352/93‑1 (MQ=255)
                                                          tCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATaa                               <  1:5527382/93‑1 (MQ=255)
                                                          tCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATaa                               >  2:2816767/1‑93 (MQ=255)
                                                          tCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATaa                               <  1:2066083/93‑1 (MQ=255)
                                                             gcgcCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGcc                            >  1:4364233/1‑93 (MQ=255)
                                                               gcCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGt                          <  2:32794/93‑1 (MQ=255)
                                                               gcCAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGt                          >  2:3328233/1‑93 (MQ=255)
                                                                 cAGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTgg                        <  2:2187252/93‑1 (MQ=255)
                                                                  aGATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTggg                       <  2:505639/93‑1 (MQ=255)
                                                                   gATCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGt                      <  2:1457950/93‑1 (MQ=255)
                                                                    aTCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTc                     <  2:2733497/93‑1 (MQ=255)
                                                                    aTCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTc                     <  1:2551936/93‑1 (MQ=255)
                                                                     tCGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCg                    >  1:3100185/1‑93 (MQ=255)
                                                                      cGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGt                   <  2:3476118/93‑1 (MQ=255)
                                                                      cGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGt                   >  1:4081164/1‑93 (MQ=255)
                                                                      cGCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGt                   <  2:1501534/93‑1 (MQ=255)
                                                                       gCTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTa                  <  1:484238/93‑1 (MQ=255)
                                                                        cTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAg                 >  1:1377186/1‑93 (MQ=255)
                                                                        cTGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAg                 >  1:318696/1‑93 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                >  1:5401115/1‑93 (MQ=255)
                                                                         tGGAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGt                >  1:1275120/1‑93 (MQ=255)
                                                                           gAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCa              <  1:1031841/93‑1 (MQ=255)
                                                                           gAAGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCa              <  2:6064428/93‑1 (MQ=255)
                                                                            aaGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAt             >  2:5277315/1‑93 (MQ=255)
                                                                             aGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            >  1:881200/1‑93 (MQ=255)
                                                                             aGTCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtt            <  2:2878262/93‑1 (MQ=255)
                                                                               tCGCGCGCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAtttt          <  1:2219098/93‑1 (MQ=255)
                                                                                cgcgcgCTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTc         <  2:443673/93‑1 (MQ=255)
                                                                                 gcgcgcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTcc        >  2:3830134/1‑93 (MQ=255)
                                                                                 gcgcgcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTcc        <  1:4740715/93‑1 (MQ=255)
                                                                                 gcgcgcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTcc        <  2:1595800/93‑1 (MQ=255)
                                                                                 gcgcgcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTcc        >  2:1977499/1‑93 (MQ=255)
                                                                                   gcgcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAg      >  2:3986962/1‑93 (MQ=255)
                                                                                     gcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCa    >  2:2340273/1‑93 (MQ=255)
                                                                                     gcTCTGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCa    >  1:339211/1‑93 (MQ=255)
                                                                                       tctGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAcc  <  2:1351452/93‑1 (MQ=255)
                                                                                       tctGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAcc  >  2:2466219/1‑93 (MQ=255)
                                                                                       tctGCTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAcc  >  1:276576/1‑93 (MQ=255)
                                                                                             |                                                                                      
TCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTGCT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC  >  NC_000913/3872358‑3872536

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCA  >  NC_000913/3872357‑3872538
                                                                                              |                                                                                         
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CT                                                                                           >  SRR4302534.5469700/1‑94 (MQ=60)
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CT                                                                                           <  SRR4302534.6419628/94‑1 (MQ=60)
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CT                                                                                           <  SRR4302534.2679191/94‑1 (MQ=60)
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CT                                                                                           >  SRR4302534.2428733/1‑94 (MQ=60)
  CGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CT‑GC                                                                                        >  SRR4302534.1836025/1‑94 (MQ=60)
  CGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CT‑GC                                                                                        >  SRR4302534.3492676/1‑94 (MQ=60)
   GTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CT‑GCC                                                                                       >  SRR4302534.2926424/1‑94 (MQ=60)
   GTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CT‑GCC                                                                                       <  SRR4302534.4649992/94‑1 (MQ=60)
    TTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCC                                                                                       >  SRR4302534.5938882/1‑94 (MQ=60)
     TAACCTCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCCCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCA                                                                                      <  SRR4302534.6347557/94‑1 (MQ=60)
     TAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCA                                                                                      >  SRR4302534.3192618/1‑94 (MQ=60)
     TAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCA                                                                                      <  SRR4302534.3933146/94‑1 (MQ=60)
     TAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCA                                                                                      >  SRR4302534.3776887/1‑94 (MQ=60)
      AAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAA                                                                                     >  SRR4302534.6501757/1‑94 (MQ=60)
       AACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAAC                                                                                    >  SRR4302534.2608775/1‑94 (MQ=60)
       AACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAAC                                                                                    >  SRR4302534.5770913/1‑94 (MQ=60)
        ACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACG                                                                                   <  SRR4302534.466601/94‑1 (MQ=60)
        ACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACG                                                                                   >  SRR4302534.3662866/1‑94 (MQ=60)
        ACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACG                                                                                   <  SRR4302534.3920751/94‑1 (MQ=60)
         CGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGG                                                                                  >  SRR4302534.689988/1‑94 (MQ=60)
          GCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGG                                                                                 >  SRR4302534.6635619/1‑94 (MQ=60)
          GCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGG                                                                                 >  SRR4302534.1388256/1‑94 (MQ=60)
           CTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGC                                                                                >  SRR4302534.2098450/1‑94 (MQ=60)
           CTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGC                                                                                >  SRR4302534.2368771/1‑94 (MQ=60)
            TTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCA                                                                               >  SRR4302534.2994898/1‑94 (MQ=60)
             TCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGCAGTCGCGCGCTCTG‑CTGCCCAACGGGCAA                                                                              >  SRR4302534.3177529/1‑94 (MQ=60)
               GCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATC                                                                            >  SRR4302534.1621873/1‑94 (MQ=60)
               GCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATC                                                                            <  SRR4302534.4406092/94‑1 (MQ=60)
               GCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATC                                                                            <  SRR4302534.258742/94‑1 (MQ=60)
                 GGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGC                                                                          >  SRR4302534.6286581/1‑94 (MQ=60)
                  GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCC                                                                         <  SRR4302534.2923186/94‑1 (MQ=60)
                  GTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCC                                                                         >  SRR4302534.6517041/1‑94 (MQ=60)
                   TCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCG                                                                        <  SRR4302534.3390643/94‑1 (MQ=60)
                    CCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGG                                                                       >  SRR4302534.4368277/1‑94 (MQ=60)
                     CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGT                                                                      >  SRR4302534.371179/1‑94 (MQ=60)
                     CTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGT                                                                      <  SRR4302534.4704147/94‑1 (MQ=60)
                      TGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCCATCGCCGGTC                                                                     >  SRR4302534.5958812/1‑94 (MQ=60)
                      TGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTT                                                                     <  SRR4302534.2162696/94‑1 (MQ=60)
                      TGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTT                                                                     <  SRR4302534.3146051/94‑1 (MQ=60)
                       GATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTC                                                                    <  SRR4302534.5532334/94‑1 (MQ=60)
                        ATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCC                                                                   <  SRR4302534.2655211/94‑1 (MQ=60)
                        ATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCC                                                                   >  SRR4302534.2886035/1‑94 (MQ=60)
                        ATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCC                                                                   <  SRR4302534.6090723/94‑1 (MQ=60)
                         TTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCA                                                                  <  SRR4302534.6375722/94‑1 (MQ=60)
                          TGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCAC                                                                 <  SRR4302534.1971844/94‑1 (MQ=60)
                          TGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCAC                                                                 >  SRR4302534.6430218/1‑94 (MQ=60)
                            TTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCA                                                               <  SRR4302534.5685839/94‑1 (MQ=60)
                            TTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCA                                                               <  SRR4302534.5260685/94‑1 (MQ=60)
                             TGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAG                                                              <  SRR4302534.5498500/94‑1 (MQ=60)
                             TGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAG                                                              >  SRR4302534.634585/1‑94 (MQ=60)
                               GCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGAT                                                            <  SRR4302534.5031159/94‑1 (MQ=60)
                                CAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATT                                                           <  SRR4302534.5427977/94‑1 (MQ=60)
                                 AATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTT                                                          >  SRR4302534.1617949/1‑94 (MQ=60)
                                 AATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTT                                                          <  SRR4302534.467871/94‑1 (MQ=60)
                                 AATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTT                                                          <  SRR4302534.475144/94‑1 (MQ=60)
                                  ATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTC                                                         >  SRR4302534.5521371/1‑94 (MQ=60)
                                  ATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTC                                                         <  SRR4302534.6103301/94‑1 (MQ=60)
                                     ATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCA                                                      >  SRR4302534.4624646/1‑94 (MQ=60)
                                      TCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAC                                                     <  SRR4302534.1524237/94‑1 (MQ=60)
                                      TCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAC                                                     >  SRR4302534.1403068/1‑94 (MQ=60)
                                      TCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCAC                                                     >  SRR4302534.6592296/1‑94 (MQ=60)
                                       CTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACT                                                    <  SRR4302534.4760907/94‑1 (MQ=60)
                                        TCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTT                                                   <  SRR4302534.1846638/94‑1 (MQ=60)
                                        TCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTT                                                   >  SRR4302534.2769242/1‑94 (MQ=60)
                                        TCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTT                                                   <  SRR4302534.2415843/94‑1 (MQ=60)
                                        TCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTT                                                   <  SRR4302534.1717566/94‑1 (MQ=60)
                                         CGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTC                                                  >  SRR4302534.93856/1‑94 (MQ=60)
                                         CGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTC                                                  >  SRR4302534.4237091/1‑94 (MQ=60)
                                            TCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCT                                               <  SRR4302534.238184/94‑1 (MQ=60)
                                            TCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCT                                               <  SRR4302534.6497372/94‑1 (MQ=60)
                                            TCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCT                                               >  SRR4302534.2557022/1‑94 (MQ=60)
                                            TCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCT                                               >  SRR4302534.2824450/1‑94 (MQ=60)
                                             CAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTC                                              <  SRR4302534.3283538/94‑1 (MQ=60)
                                              AGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCA                                             >  SRR4302534.6558610/1‑94 (MQ=60)
                                              AGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCA                                             >  SRR4302534.6648217/1‑94 (MQ=60)
                                              AGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCA                                             <  SRR4302534.3820407/94‑1 (MQ=60)
                                                CGCCGATTCAATCTGCGCCAGATCGCTGGCAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTACCACCAGATTTCGCACTTCGCTCATG                                           >  SRR4302534.2503703/1‑94 (MQ=60)
                                                CGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATG                                           >  SRR4302534.2012124/1‑94 (MQ=60)
                                                 GCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                          <  SRR4302534.2986276/94‑1 (MQ=60)
                                                 GCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                          >  SRR4302534.6117108/1‑94 (MQ=60)
                                                 GCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGG                                          <  SRR4302534.5198327/94‑1 (MQ=60)
                                                  CCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGC                                         <  SRR4302534.3019041/94‑1 (MQ=60)
                                                       TCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGA                                    <  SRR4302534.4762420/94‑1 (MQ=60)
                                                       TCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGA                                    <  SRR4302534.548406/94‑1 (MQ=60)
                                                        CAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGAT                                   >  SRR4302534.1524407/1‑94 (MQ=60)
                                                         AATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATA                                  <  SRR4302534.2088061/94‑1 (MQ=60)
                                                          ATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAA                                 <  SRR4302534.6235700/94‑1 (MQ=60)
                                                          ATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAA                                 <  SRR4302534.5124471/94‑1 (MQ=60)
                                                           TCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAG                                <  SRR4302534.2120112/94‑1 (MQ=60)
                                                           TCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAG                                >  SRR4302534.4844551/1‑94 (MQ=60)
                                                           TCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAG                                <  SRR4302534.5676558/94‑1 (MQ=60)
                                                             TGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                              <  SRR4302534.2223327/94‑1 (MQ=60)
                                                             TGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCC                              >  SRR4302534.4481909/1‑94 (MQ=60)
                                                               CGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGT                            <  SRR4302534.2287771/94‑1 (MQ=60)
                                                               CGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGT                            >  SRR4302534.1703089/1‑94 (MQ=60)
                                                                GCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTG                           >  SRR4302534.488150/1‑94 (MQ=60)
                                                                GCCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTG                           >  SRR4302534.429503/1‑94 (MQ=60)
                                                                 CCAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGG                          <  SRR4302534.5003990/94‑1 (MQ=60)
                                                                  CAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGG                         >  SRR4302534.6478974/1‑94 (MQ=60)
                                                                  CAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGG                         <  SRR4302534.1840927/94‑1 (MQ=60)
                                                                  CAGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGG                         >  SRR4302534.3628311/1‑94 (MQ=60)
                                                                   AGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                        >  SRR4302534.739261/1‑94 (MQ=60)
                                                                   AGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                        >  SRR4302534.569536/1‑94 (MQ=60)
                                                                   AGATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGT                        >  SRR4302534.1170662/1‑94 (MQ=60)
                                                                    GATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                       <  SRR4302534.2958253/94‑1 (MQ=60)
                                                                    GATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTC                       >  SRR4302534.3013832/1‑94 (MQ=60)
                                                                     ATCGCTGNAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTNCGNNNNTGGCACTGATAANCNNTGGGTCG                      >  SRR4302534.2959571/1‑94 (MQ=60)
                                                                     ATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCG                      >  SRR4302534.3181736/1‑94 (MQ=60)
                                                                     ATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCG                      <  SRR4302534.2619592/94‑1 (MQ=60)
                                                                     ATCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCG                      <  SRR4302534.5604235/94‑1 (MQ=60)
                                                                      TCGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGT                     >  SRR4302534.4190714/1‑94 (MQ=60)
                                                                       CGCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTA                    >  SRR4302534.3979052/1‑94 (MQ=60)
                                                                        GCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAG                   >  SRR4302534.1413469/1‑94 (MQ=60)
                                                                        GCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAG                   >  SRR4302534.326907/1‑94 (MQ=60)
                                                                        GCTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAG                   <  SRR4302534.496368/94‑1 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTGCCG‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGGACTGATAAGGCGTGGGACGTAGC                  >  SRR4302534.5168973/1‑94 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGT                  >  SRR4302534.1308682/1‑94 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGT                  >  SRR4302534.3152937/1‑94 (MQ=60)
                                                                         CTGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGT                  >  SRR4302534.5546493/1‑94 (MQ=60)
                                                                          TGGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTC                 <  SRR4302534.1928319/94‑1 (MQ=60)
                                                                           GGAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCA                <  SRR4302534.5051180/94‑1 (MQ=60)
                                                                           GGAAGGCGCGCGCTCTG‑CTGCCTAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCTCTCATGGCACTGATAAGCCGTGGGTCGTAGTCA                <  SRR4302534.2797403/94‑1 (MQ=60)
                                                                            GAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAT               <  SRR4302534.1058432/94‑1 (MQ=60)
                                                                            GAAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCAT               >  SRR4302534.5584422/1‑94 (MQ=60)
                                                                             AAGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATT              >  SRR4302534.903480/1‑94 (MQ=60)
                                                                              AGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTT             <  SRR4302534.4584832/94‑1 (MQ=60)
                                                                              AGTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTT             >  SRR4302534.2517881/1‑94 (MQ=60)
                                                                               GTCGCTCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGCTAAACCGTGGGTCGTAGTCATTTT            <  SRR4302534.4488670/94‑1 (MQ=60)
                                                                               GTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTT            <  SRR4302534.3928412/94‑1 (MQ=60)
                                                                               GTCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTT            <  SRR4302534.5404694/94‑1 (MQ=60)
                                                                                TCGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTC           <  SRR4302534.2277875/94‑1 (MQ=60)
                                                                                 CGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTTGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCC          >  SRR4302534.3194115/1‑94 (MQ=60)
                                                                                 CGCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCC          >  SRR4302534.590882/1‑94 (MQ=60)
                                                                                  GCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCA         <  SRR4302534.5078806/94‑1 (MQ=60)
                                                                                  GCGCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCA         <  SRR4302534.4869410/94‑1 (MQ=60)
                                                                                    GCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGC       >  SRR4302534.82066/1‑94 (MQ=60)
                                                                                    GCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGC       <  SRR4302534.2678282/94‑1 (MQ=60)
                                                                                    GCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGC       >  SRR4302534.4024212/1‑94 (MQ=60)
                                                                                    GCGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGC       <  SRR4302534.4998432/94‑1 (MQ=60)
                                                                                     CGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCA      <  SRR4302534.491682/94‑1 (MQ=60)
                                                                                     CGCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCA      >  SRR4302534.347889/1‑94 (MQ=60)
                                                                                      GCTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCAC     <  SRR4302534.5304436/94‑1 (MQ=60)
                                                                                       CTCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACC    >  SRR4302534.283739/1‑94 (MQ=60)
                                                                                        TCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGTTAAGCCGTGGGTCGTAGTCATTTTCCAGCACCC   >  SRR4302534.5752051/1‑94 (MQ=60)
                                                                                        TCTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCC   >  SRR4302534.833030/1‑94 (MQ=60)
                                                                                         CTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCA  <  SRR4302534.2463972/94‑1 (MQ=60)
                                                                                         CTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCA  >  SRR4302534.84207/1‑94 (MQ=60)
                                                                                         CTG‑CTGCCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCA  <  SRR4302534.3531042/94‑1 (MQ=60)
                                                                                              |                                                                                         
TTCGTTAAACGCTTCGCGGTCCTGATTGTTGGCAATCATCTCGTTCAGCGCCGATTCAATCTGCGCCAGATCGCTGGAAGTCGCGCGCTCTG‑CT‑CCCAACGGGCAATCGCCGGTTCCACCAGATTTCGCACTTCGCTCATGGCACTGATAAGCCGTGGGTCGTAGTCATTTTCCAGCACCCA  >  NC_000913/3872357‑3872538

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑