Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F1 I1 R1	744	338.8	17650688	97.8%	17262372	93.0

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,923,014	A→G	I83T (ATA→ACA)	gidA ←	glucose‑inhibited division

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,923,014	0	A	G	99.3%	1009.2 / ‑5.1	290	I83T (ATA→ACA)	gidA	glucose‑inhibited division
Reads supporting (aligned to +/- strand): ref base A (1/1); new base G (133/155); total (134/156)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 302 total aligned reads displayed.

TCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATA  >  NC_000913/3922924‑3923103

tcCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTc                                                                                         <  1:6390629/93‑1 (MQ=255)

 ccAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTcc                                                                                        >  1:4444922/1‑93 (MQ=255)

  cAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCt                                                                                       >  2:7950768/1‑93 (MQ=255)

  cAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCt                                                                                       >  2:104583/1‑93 (MQ=255)

    gcgcCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTaa                                                                                     <  2:59372/93‑1 (MQ=255)

      gcCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAAc                                                                                   <  2:7421941/93‑1 (MQ=255)

      gcCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAAc                                                                                   <  2:2787579/93‑1 (MQ=255)

        cgtacgtaCCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTg                                                                                 <  2:3996587/93‑1 (MQ=255)

        cgtacgtaCCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCGTGCGTTTAGTGTCCGAAACTg                                                                                 <  1:3061958/93‑1 (MQ=255)

         gtacgtacCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTgg                                                                                >  2:1784753/1‑93 (MQ=255)

              tacCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATAcc                                                                           <  1:162382/93‑1 (MQ=255)

              tacCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATAcc                                                                           >  1:5447991/1‑93 (MQ=255)

              tacCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATAcc                                                                           <  1:3124860/93‑1 (MQ=255)

              tacCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATAcc                                                                           <  1:4526562/93‑1 (MQ=255)

              tacCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTACTTTGCTTGCGTTTAGTGTCCTAAACTGGATAcc                                                                           >  2:3015471/1‑93 (MQ=255)

               tccGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATAccc                                                                          <  1:7177333/92‑1 (MQ=255)

               acCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATAccc                                                                          <  2:3253340/93‑1 (MQ=255)

               acCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATAccc                                                                          >  2:1372975/1‑93 (MQ=255)

                cccccTGCCGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCg                                                                         <  2:2217835/90‑1 (MQ=255)

                   ccTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCt                                                                      >  1:5755262/1‑93 (MQ=255)

                   ccTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCt                                                                      <  2:8145046/93‑1 (MQ=255)

                    cTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTg                                                                     >  1:1100252/1‑93 (MQ=255)

                    cTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTg                                                                     <  2:6632238/93‑1 (MQ=255)

                     tGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTga                                                                    >  1:1134037/1‑93 (MQ=255)

                      gACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTgat                                                                   <  1:1044589/93‑1 (MQ=255)

                      gACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTgat                                                                   >  2:4567492/1‑93 (MQ=255)

                      gACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTgat                                                                   <  2:7778106/93‑1 (MQ=255)

                         ggTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTgatcga                                                                <  2:2175723/93‑1 (MQ=255)

                          ggagagCACCCGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTgatcgat                                                               <  2:3528515/91‑1 (MQ=255)

                           tAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTgatcgatc                                                              <  2:4834420/93‑1 (MQ=255)

                           tAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTgatcgatc                                                              <  1:4763754/93‑1 (MQ=255)

                             gagCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGc                                                            >  2:2954731/1‑93 (MQ=255)

                              agCCCACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCt                                                           <  2:6603811/93‑1 (MQ=255)

                              agCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCt                                                           <  1:7962744/93‑1 (MQ=255)

                                 acacGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTc                                                        >  1:6123228/1‑93 (MQ=255)

                                 acacGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTc                                                        <  2:3429395/93‑1 (MQ=255)

                                 acacGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTc                                                        >  1:7403245/1‑93 (MQ=255)

                                  cacGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCg                                                       >  2:6051695/1‑93 (MQ=255)

                                     gATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCa                                                    <  1:1993460/93‑1 (MQ=255)

                                     caTCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATCCCCGCCTGATCGATCGCTTTCGCCa                                                    <  1:6742553/92‑1 (MQ=255)

                                      aTCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCac                                                   >  1:6242970/1‑92 (MQ=255)

                                      aTCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCAt                                                   <  2:5134636/93‑1 (MQ=255)

                                      aTCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCAt                                                   <  2:7986189/93‑1 (MQ=255)

                                       tCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATc                                                  <  2:206825/93‑1 (MQ=255)

                                        ccGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCa                                                 <  2:5176759/93‑1 (MQ=255)

                                         cGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAg                                                <  1:2058612/93‑1 (MQ=255)

                                           ccTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAc                                              >  1:4034739/1‑93 (MQ=255)

                                           ccTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAc                                              >  2:1707444/1‑93 (MQ=255)

                                           ccTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAc                                              <  1:950923/93‑1 (MQ=255)

                                           ccTGAGCGCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAc                                              <  1:3171065/93‑1 (MQ=255)

                                            cTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAcc                                             <  2:4598689/93‑1 (MQ=255)

                                             tGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTTGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAccg                                            >  2:7524633/1‑93 (MQ=255)

                                              gAGTTCGCGTACCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAccgc                                           <  2:6208826/93‑1 (MQ=255)

                                                gCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAccgccg                                         >  2:4321664/1‑93 (MQ=255)

                                                gCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAccgccg                                         >  1:8169645/1‑93 (MQ=255)

                                                 cTCGGTGAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGa                                        <  2:5672008/93‑1 (MQ=255)

                                                  gcGGGTACCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAg                                       <  2:7173937/92‑1 (MQ=255)

                                                   cGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGt                                      >  2:7584573/1‑93 (MQ=255)

                                                   cGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGt                                      <  1:8593665/93‑1 (MQ=255)

                                                     ggTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGc                                    >  1:6564945/1‑93 (MQ=255)

                                                      gTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCa                                   <  1:5312692/93‑1 (MQ=255)

                                                        aGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATc                                 <  2:5267919/93‑1 (MQ=255)

                                                            cgAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCAct                             >  1:4386489/1‑93 (MQ=255)

                                                             gAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCActt                            >  2:4436168/1‑93 (MQ=255)

                                                              aaCCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCActtc                           >  1:6319631/1‑93 (MQ=255)

                                                              aaCCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCActtc                           >  2:1857695/1‑93 (MQ=255)

                                                               aCCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCActtct                          >  2:702488/1‑93 (MQ=255)

                                                                ccgccgGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCActtctt                         >  1:3346567/1‑93 (MQ=255)

                                                                  gccgGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCtttt                       >  2:3199325/1‑93 (MQ=255)

                                                                   ccgGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTa                      <  1:3748831/93‑1 (MQ=255)

                                                                    cgGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTAc                     <  1:5034483/93‑1 (MQ=255)

                                                                     ggTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTGCTTTAcc                    >  1:8276426/1‑93 (MQ=255)

                                                                     ggTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTAcc                    >  2:2555409/1‑93 (MQ=255)

                                                                      gTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCa                   >  2:6522660/1‑93 (MQ=255)

                                                                      gTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCa                   >  2:5340330/1‑93 (MQ=255)

                                                                      gTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCa                   <  2:1229239/93‑1 (MQ=255)

                                                                      gTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCa                   >  1:6917382/1‑93 (MQ=255)

                                                                        ccTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGa                 >  1:3424494/1‑93 (MQ=255)

                                                                        ccTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGa                 >  2:1933393/1‑93 (MQ=255)

                                                                        ccTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGa                 >  2:6427880/1‑93 (MQ=255)

                                                                         cTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGAt                >  1:1363846/1‑93 (MQ=255)

                                                                         cTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGAt                >  2:1656834/1‑93 (MQ=255)

                                                                         cTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGAt                >  1:2994238/1‑93 (MQ=255)

                                                                         cTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGAt                >  1:22905/1‑93 (MQ=255)

                                                                          tttGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATg               >  2:6107380/1‑93 (MQ=255)

                                                                          tttGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATg               <  2:856179/93‑1 (MQ=255)

                                                                             gcttgcGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTcc            >  1:7558883/1‑93 (MQ=255)

                                                                              tttgcCTTTGGTGTCCTAAACGGCATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTccc           <  2:7345493/92‑1 (MQ=255)

                                                                              cttgcGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTccc           >  1:3816095/1‑93 (MQ=255)

                                                                               ttgcGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCt          <  1:2661332/93‑1 (MQ=255)

                                                                               ttgcGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCt          <  1:1242129/93‑1 (MQ=255)

                                                                                 gcGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTc        >  2:3109114/1‑93 (MQ=255)

                                                                                 gcGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTc        >  1:3486356/1‑93 (MQ=255)

                                                                                    tttAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCa     >  1:8677574/1‑93 (MQ=255)

                                                                                     ttAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCTACTTCTTTTACCAGATGTCCCTTCCCaa    >  1:3936018/1‑93 (MQ=255)

                                                                                      tAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAAt   >  2:499185/1‑93 (MQ=255)

                                                                                      tAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAAt   >  1:2836668/1‑93 (MQ=255)

                                                                                      tAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAAt   <  2:3656035/93‑1 (MQ=255)

                                                                                      tAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAAt   >  1:7468070/1‑93 (MQ=255)

                                                                                       aGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATa  <  1:496698/93‑1 (MQ=255)

TCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATA  >  NC_000913/3922924‑3923103

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 20 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

TTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCG  >  NC_000913/3922921‑3923106

TTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCCCACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTG                                                                                              <  SRR4302541.6857257/94‑1 (MQ=60)

TTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCGTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTG                                                                                              <  SRR4302541.1125332/94‑1 (MQ=60)

TTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTG                                                                                              >  SRR4302541.2564767/1‑94 (MQ=60)

TTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTG                                                                                              <  SRR4302541.3479727/94‑1 (MQ=60)

  CTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTC                                                                                            <  SRR4302541.8315750/94‑1 (MQ=60)

   TCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCC                                                                                           <  SRR4302541.6455870/94‑1 (MQ=60)

   TCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCC                                                                                           >  SRR4302541.4489613/1‑94 (MQ=60)

    CCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCT                                                                                          <  SRR4302541.8505319/94‑1 (MQ=60)

    CCAGCGCCGGACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCT                                                                                          <  SRR4302541.2841116/94‑1 (MQ=60)

     CAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTA                                                                                         <  SRR4302541.6499326/94‑1 (MQ=60)

     CAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTA                                                                                         >  SRR4302541.7673253/1‑94 (MQ=60)

       GCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAA                                                                                       <  SRR4302541.3082838/94‑1 (MQ=60)

        CGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAAC                                                                                      <  SRR4302541.3384084/94‑1 (MQ=60)

         GCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACT                                                                                     >  SRR4302541.2367016/1‑94 (MQ=60)

          CCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTG                                                                                    >  SRR4302541.8765978/1‑94 (MQ=60)

          CCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTG                                                                                    >  SRR4302541.7024956/1‑94 (MQ=60)

           CGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGG                                                                                   >  SRR4302541.3394424/1‑94 (MQ=60)

           CGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGG                                                                                   >  SRR4302541.6325608/1‑94 (MQ=60)

           CGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGG                                                                                   <  SRR4302541.3092986/94‑1 (MQ=60)

           CGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCGTGCGTTTAGTGTCCGAAACTGG                                                                                   <  SRR4302541.3092987/94‑1 (MQ=60)

             TACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGAT                                                                                 <  SRR4302541.5543389/94‑1 (MQ=60)

              TCGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATA                                                                                <  SRR4302541.7521817/94‑1 (MQ=60)

               CGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGGAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATAC                                                                               >  SRR4302541.3304188/1‑94 (MQ=60)

                GTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACC                                                                              <  SRR4302541.5490656/94‑1 (MQ=60)

                GTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACC                                                                              <  SRR4302541.7399511/94‑1 (MQ=60)

                GTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACC                                                                              >  SRR4302541.5503891/1‑94 (MQ=60)

                 TACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCC                                                                             <  SRR4302541.163602/94‑1 (MQ=60)

                 TACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCC                                                                             <  SRR4302541.3156462/94‑1 (MQ=60)

                 TACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCC                                                                             <  SRR4302541.4572250/94‑1 (MQ=60)

                  TCCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCG                                                                            <  SRR4302541.7253883/94‑1 (MQ=60)

                    CGCCTGACGGTAGAGCACACGATCCGCCTGAGGTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCC                                                                          <  SRR4302541.643366/94‑1 (MQ=60)

                    CGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCC                                                                          >  SRR4302541.7491850/1‑94 (MQ=60)

                    CGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGGAGCGCGAACCGCCTGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATAACCGCC                                                                          >  SRR4302541.2192325/1‑94 (MQ=60)

                     GCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCT                                                                         >  SRR4302541.5813848/1‑94 (MQ=60)

                      CCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTG                                                                        >  SRR4302541.1110553/1‑94 (MQ=60)

                      CCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTG                                                                        >  SRR4302541.1110552/1‑94 (MQ=60)

                       CTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGA                                                                       >  SRR4302541.1144767/1‑94 (MQ=60)

                       CTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGA                                                                       <  SRR4302541.5455556/94‑1 (MQ=60)

                        TGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGAT                                                                      <  SRR4302541.6696306/94‑1 (MQ=60)

                         GACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATC                                                                     <  SRR4302541.1054268/94‑1 (MQ=60)

                         GACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATC                                                                     <  SRR4302541.1518513/94‑1 (MQ=60)

                          ACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCG                                                                    <  SRR4302541.8701022/94‑1 (MQ=60)

                           CGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGA                                                                   >  SRR4302541.8096264/1‑94 (MQ=60)

                           CGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGA                                                                   <  SRR4302541.826522/94‑1 (MQ=60)

                            GGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGAT                                                                  <  SRR4302541.1162232/94‑1 (MQ=60)

                            GGGAGACCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGAT                                                                  <  SRR4302541.1162233/94‑1 (MQ=60)

                             GTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATC                                                                 <  SRR4302541.8202309/94‑1 (MQ=60)

                             GTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATC                                                                 <  SRR4302541.1172449/94‑1 (MQ=60)

                              TAGAGCACGCGATCCGCCTTAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCG                                                                <  SRR4302541.8902129/94‑1 (MQ=60)

                              TAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCG                                                                <  SRR4302541.4812404/94‑1 (MQ=60)

                                 AGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTT                                                             <  SRR4302541.8050490/94‑1 (MQ=60)

                                   CACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTC                                                           >  SRR4302541.6185770/1‑94 (MQ=60)

                                   CACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTC                                                           >  SRR4302541.7482421/1‑94 (MQ=60)

                                   CACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTC                                                           <  SRR4302541.3703860/94‑1 (MQ=60)

                                   CACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTC                                                           <  SRR4302541.34769/94‑1 (MQ=60)

                                       CGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCA                                                       <  SRR4302541.1081649/94‑1 (MQ=60)

                                        GATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCAT                                                      <  SRR4302541.2012744/94‑1 (MQ=60)

                                        GATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCAT                                                      >  SRR4302541.1692123/1‑94 (MQ=60)

                                        GATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCAC                                                      >  SRR4302541.6306750/1‑94 (MQ=60)

                                        CATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATCCCCGCCTGATCGATCGCTTTCGCCAT                                                      <  SRR4302541.6814584/94‑1 (MQ=60)

                                         ATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATC                                                     >  SRR4302541.1134886/1‑94 (MQ=60)

                                         ATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATC                                                     >  SRR4302541.109577/1‑94 (MQ=60)

                                          TCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCA                                                    <  SRR4302541.3340910/94‑1 (MQ=60)

                                           CCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAG                                                   >  SRR4302541.3095381/1‑94 (MQ=60)

                                           CCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAG                                                   >  SRR4302541.3099643/1‑94 (MQ=60)

                                           CCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAG                                                   >  SRR4302541.1960994/1‑94 (MQ=60)

                                            CGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGA                                                  <  SRR4302541.6418529/94‑1 (MQ=60)

                                            CGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGA                                                  <  SRR4302541.2078493/94‑1 (MQ=60)

                                             GCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAC                                                 >  SRR4302541.1312280/1‑94 (MQ=60)

                                             GCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAC                                                 >  SRR4302541.3894847/1‑94 (MQ=60)

                                             GCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAC                                                 >  SRR4302541.4074587/1‑94 (MQ=60)

                                             GCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAC                                                 >  SRR4302541.206631/1‑94 (MQ=60)

                                             CCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGAC                                                 <  SRR4302541.4973626/94‑1 (MQ=60)

                                              CCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACC                                                <  SRR4302541.959940/94‑1 (MQ=60)

                                              CCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACC                                                <  SRR4302541.5011431/94‑1 (MQ=60)

                                              CCTGAGCGCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACC                                                <  SRR4302541.3203109/94‑1 (MQ=60)

                                               CTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCG                                               <  SRR4302541.3202742/94‑1 (MQ=60)

                                               CTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCC                                               >  SRR4302541.2055535/1‑94 (MQ=60)

                                                CGAGCTCGGGTAGCGGGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGA                                              <  SRR4302541.316552/94‑1 (MQ=60)

                                                 GAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCC                                             <  SRR4302541.2919501/94‑1 (MQ=60)

                                                  AGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCG                                            >  SRR4302541.763203/1‑94 (MQ=60)

                                                  AGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCG                                            >  SRR4302541.8258858/1‑94 (MQ=60)

                                                    CTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTTGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAG                                          <  SRR4302541.7605034/94‑1 (MQ=60)

                                                      CGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTG                                        >  SRR4302541.5969094/1‑94 (MQ=60)

                                                      CGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTG                                        <  SRR4302541.3786402/94‑1 (MQ=60)

                                                      CGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTG                                        <  SRR4302541.8685874/94‑1 (MQ=60)

                                                       GGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGC                                       >  SRR4302541.6632089/1‑94 (MQ=60)

                                                        GGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCA                                      <  SRR4302541.1365106/94‑1 (MQ=60)

                                                         GTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCAT                                     <  SRR4302541.5367378/94‑1 (MQ=60)

                                                          GAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATC                                    <  SRR4302541.7624970/94‑1 (MQ=60)

                                                              GCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACT                                >  SRR4302541.4430361/1‑94 (MQ=60)

                                                              GCGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACT                                <  SRR4302541.4042861/94‑1 (MQ=60)

                                                               CGAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTT                               >  SRR4302541.4361310/1‑94 (MQ=60)

                                                                GAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTC                              >  SRR4302541.2222751/1‑94 (MQ=60)

                                                                GAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTC                              >  SRR4302541.1550150/1‑94 (MQ=60)

                                                                GAACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTC                              >  SRR4302541.6384244/1‑94 (MQ=60)

                                                                  ACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTT                            <  SRR4302541.6836003/94‑1 (MQ=60)

                                                                  ACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTT                            >  SRR4302541.3380017/1‑94 (MQ=60)

                                                                  ACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTT                            >  SRR4302541.4688547/1‑94 (MQ=60)

                                                                  ACCGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTT                            <  SRR4302541.4101383/94‑1 (MQ=60)

                                                                    CGCCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTT                          >  SRR4302541.6296132/1‑94 (MQ=60)

                                                                      CCGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTAC                        <  SRR4302541.3786554/94‑1 (MQ=60)

                                                                       CGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGCTCAATCGCTTTCGCgatccgcgcgcagagtgcatcccatctctttacc                       >  SRR4302541.8366474/1‑60 (MQ=60)

                                                                       CGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTGCTTTACC                       >  SRR4302541.8366476/1‑94 (MQ=60)

                                                                       CGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACC                       >  SRR4302541.5182233/1‑94 (MQ=60)

                                                                       CGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACC                       <  SRR4302541.5086278/94‑1 (MQ=60)

                                                                       CGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACC                       <  SRR4302541.6964401/94‑1 (MQ=60)

                                                                       CGGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACC                       <  SRR4302541.6144976/94‑1 (MQ=60)

                                                                        GGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCA                      <  SRR4302541.8604028/94‑1 (MQ=60)

                                                                        GGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCA                      <  SRR4302541.702376/94‑1 (MQ=60)

                                                                        GGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCA                      >  SRR4302541.6991203/1‑94 (MQ=60)

                                                                        GGTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAATGCATCCACTTCTTTTACCA                      >  SRR4302541.7461203/1‑94 (MQ=60)

                                                                         GTCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAG                     >  SRR4302541.3518247/1‑94 (MQ=60)

                                                                         GTCCTTTGCTTGCGTTTAGTGTACTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAG                     >  SRR4302541.5740631/1‑94 (MQ=60)

                                                                          TCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGA                    >  SRR4302541.3458686/1‑94 (MQ=60)

                                                                          TCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGA                    >  SRR4302541.8360081/1‑94 (MQ=60)

                                                                          TCCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGA                    <  SRR4302541.667899/94‑1 (MQ=60)

                                                                           CCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGAT                   >  SRR4302541.23091/1‑94 (MQ=60)

                                                                           CCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGAT                   >  SRR4302541.3024459/1‑94 (MQ=60)

                                                                           CCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGAT                   >  SRR4302541.1377287/1‑94 (MQ=60)

                                                                           CCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGAT                   >  SRR4302541.4564616/1‑94 (MQ=60)

                                                                           CCTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGAT                   <  SRR4302541.90043/94‑1 (MQ=60)

                                                                           CCTTTGCTTGCGTGTAGTGTCCTAAACTGGATACCCGCCTGATCCATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAAAT                   >  SRR4302541.3024458/1‑94 (MQ=60)

                                                                            CTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATG                  >  SRR4302541.3833787/1‑94 (MQ=60)

                                                                            CTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATG                  >  SRR4302541.5122037/1‑94 (MQ=60)

                                                                            CTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATG                  >  SRR4302541.3833786/1‑94 (MQ=60)

                                                                            CTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATG                  <  SRR4302541.4464695/94‑1 (MQ=60)

                                                                            CTTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATG                  <  SRR4302541.4464694/94‑1 (MQ=60)

                                                                             TTTGCTTGCGTTTAGTGTCCTAAACTTGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGGGTGCATCCGCTTCTTTTACCAGATGT                 >  SRR4302541.2732055/1‑94 (MQ=60)

                                                                             TTTGCTTGCGTTTAGTGTCCTAAACTGGATCCCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGT                 <  SRR4302541.6619329/94‑1 (MQ=60)

                                                                             TTTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGT                 <  SRR4302541.6343953/94‑1 (MQ=60)

                                                                              TTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTC                >  SRR4302541.4495070/1‑94 (MQ=60)

                                                                              TTGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTC                >  SRR4302541.2537998/1‑94 (MQ=60)

                                                                               TGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCC               >  SRR4302541.7639666/1‑94 (MQ=60)

                                                                               TGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCC               >  SRR4302541.5066023/1‑94 (MQ=60)

                                                                               TGCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTCACCAGATGTGC               >  SRR4302541.5066027/1‑94 (MQ=60)

                                                                                GCTTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCC              >  SRR4302541.3854536/1‑94 (MQ=60)

                                                                                  TTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTT            <  SRR4302541.2687796/94‑1 (MQ=60)

                                                                                  TTGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTT            <  SRR4302541.1254413/94‑1 (MQ=60)

                                                                                   TGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTC           >  SRR4302541.3521268/1‑94 (MQ=60)

                                                                                   TGCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTC           >  SRR4302541.4683651/1‑94 (MQ=60)

                                                                                    GCGTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCC          <  SRR4302541.4008422/94‑1 (MQ=60)

                                                                                      GTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCA        >  SRR4302541.8770425/1‑94 (MQ=60)

                                                                                      GTTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCA        >  SRR4302541.6258602/1‑94 (MQ=60)

                                                                                       TTTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCTACTTCTTTTACCAGATGTCCCTTCCCAA       >  SRR4302541.3975204/1‑94 (MQ=60)

                                                                                        TTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAAT      >  SRR4302541.7547920/1‑94 (MQ=60)

                                                                                        TTAGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAAT      >  SRR4302541.2865216/1‑94 (MQ=60)

                                                                                         TAGTGGCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATA     <  SRR4302541.2399973/94‑1 (MQ=60)

                                                                                          AGTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATAC    <  SRR4302541.501377/94‑1 (MQ=60)

                                                                                           GTGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGTCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACC   <  SRR4302541.278802/94‑1 (MQ=60)

                                                                                            TGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCG  <  SRR4302541.1320950/94‑1 (MQ=60)

                                                                                            TGTCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCG  <  SRR4302541.1705910/94‑1 (MQ=60)

TTCTCCAGCGCCGTACGTACCGCCTGACGGTAGAGCACACGATCCGCCTGAGCTCGGGTAGCGCGAACCGCCGGTCCTTTGCTTGCGTTTAGTATCCTAAACTGGATACCCGCCTGATCGATCGCTTTCGCCATCAGACCGCCGAGTGCATCCACTTCTTTTACCAGATGTCCCTTCCCAATACCG  >  NC_000913/3922921‑3923106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 2 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 33 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`