Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,944,676 2 bp→GC intergenic (+20/+75) trpT → / ← yifDA tRNA‑Trp/transcription regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,944,6760CG100.0% 1848.4 / NA 533intergenic (+20/+76)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (260/273);  total (260/273)
*NC_0009133,944,6770GC99.8% 1877.1 / NA 533intergenic (+21/+75)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (260/272);  minor base A (0/1);  total (260/273)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 545 total aligned reads displayed.

AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTC  >  NC_000913/3944589‑3944763
                                                                                       ||                                                                                      
  ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                  <  2:1155197/93‑1 (MQ=255)
   tCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAt                                                                                 <  2:3217735/93‑1 (MQ=255)
   tCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAt                                                                                 <  1:4475955/93‑1 (MQ=255)
    cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtt                                                                                >  1:5929535/1‑93 (MQ=255)
    cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAAGCATCCTTAGCGAAAGCTAAGGAtt                                                                                <  1:7039788/93‑1 (MQ=255)
      aTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttt                                                                              <  2:1859445/93‑1 (MQ=255)
        tGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttt                                                                            <  1:8222474/93‑1 (MQ=255)
          gTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttttt                                                                          <  2:1604982/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  1:1664787/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  1:403736/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  1:3882313/93‑1 (MQ=255)
             gagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATc                                                                       >  2:3603037/1‑93 (MQ=255)
              agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCt                                                                      >  2:6808495/1‑93 (MQ=255)
               gCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTg                                                                     <  2:7039704/93‑1 (MQ=255)
               gCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTg                                                                     <  2:7473499/93‑1 (MQ=255)
                cACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGa                                                                    <  1:7454941/93‑1 (MQ=255)
                 aCCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGaa                                                                   >  1:3225594/1‑93 (MQ=255)
                   cGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAt                                                                 <  1:5071708/93‑1 (MQ=255)
                    ggTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATa                                                                <  2:8598176/93‑1 (MQ=255)
                     gTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATaa                                                               <  2:4386945/93‑1 (MQ=255)
                      tctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAc                                                              <  2:4231581/93‑1 (MQ=255)
                      tctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAc                                                              <  2:1357488/93‑1 (MQ=255)
                       ctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAcc                                                             >  2:5577795/1‑93 (MQ=255)
                        tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCTCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAccc                                                            <  2:2568050/93‑1 (MQ=255)
                        tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAccc                                                            <  1:5509954/93‑1 (MQ=255)
                        tcCAAAACCGGGCGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAcca                                                            >  2:5272121/1‑92 (MQ=255)
                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           <  1:7679192/93‑1 (MQ=255)
                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           >  2:8193414/1‑93 (MQ=255)
                          cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                          >  1:2791281/1‑93 (MQ=255)
                          cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                          >  1:2464398/1‑93 (MQ=255)
                          cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                          <  2:7995258/93‑1 (MQ=255)
                           aaaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctct                                                         <  1:2646395/93‑1 (MQ=255)
                            aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCtctc                                                        >  1:4297670/1‑93 (MQ=255)
                                cGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGaa                                                    <  2:775033/93‑1 (MQ=255)
                                 gggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAg                                                   >  1:7538783/1‑93 (MQ=255)
                                     gtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTaaa                                               <  2:6586834/93‑1 (MQ=255)
                                     gtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTaaa                                               >  2:4137968/1‑93 (MQ=255)
                                     gtTGAGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTaaa                                               <  1:239580/93‑1 (MQ=255)
                                      tggggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAt                                              <  1:3935059/91‑1 (MQ=255)
                                           aGTTCGAGTCTCTCCGCCCCTGCGAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTc                                         <  2:4749005/93‑1 (MQ=255)
                                           aGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTc                                         <  1:499215/93‑1 (MQ=255)
                                            gTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCt                                        >  2:2686977/1‑93 (MQ=255)
                                             ttCGAGTCTCTCTGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTa                                       >  2:1207425/1‑93 (MQ=255)
                                             ttCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTa                                       <  1:456640/93‑1 (MQ=255)
                                             ttCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTa                                       >  2:4849198/1‑93 (MQ=255)
                                              tCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAc                                      <  1:2105055/93‑1 (MQ=255)
                                                gAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCg                                    >  1:3003313/1‑93 (MQ=255)
                                                 aGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCgg                                   >  1:8122179/1‑93 (MQ=255)
                                                  gTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGc                                  >  2:3931621/1‑93 (MQ=255)
                                                    ctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCAt                                >  1:7221410/1‑93 (MQ=255)
                                                    ctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCAt                                <  2:754253/93‑1 (MQ=255)
                                                     tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATc                               <  2:2790851/93‑1 (MQ=255)
                                                     tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCATTCTACCGGCATc                               >  1:5410669/1‑93 (MQ=255)
                                                      ctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTACCCTCTCCGAAGTAAATCCTTCTACCGGCATcc                              >  1:1062280/1‑93 (MQ=255)
                                                      ctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATcc                              <  1:1371945/93‑1 (MQ=255)
                                                       tcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCTGCATCCt                             >  2:8101302/1‑93 (MQ=255)
                                                       tcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCt                             >  1:5606459/1‑93 (MQ=255)
                                                       cccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCt                             <  1:1173880/92‑1 (MQ=255)
                                                        ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCtt                            <  1:892671/93‑1 (MQ=255)
                                                        ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCtt                            <  1:8470977/93‑1 (MQ=255)
                                                         cGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAGTCCTTCTACCGGCATCCTTg                           >  2:5886375/1‑93 (MQ=255)
                                                          tccccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgc                          >  2:2888088/2‑93 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATACCCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        <  2:7163342/93‑1 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAATCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        >  1:1843044/1‑93 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        <  2:2374684/93‑1 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        <  2:3029624/93‑1 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        >  1:1518250/1‑93 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        <  2:8313155/93‑1 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        <  1:6776441/93‑1 (MQ=255)
                                                             ccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccag                       <  2:222217/93‑1 (MQ=255)
                                                             ccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccag                       >  1:668626/1‑93 (MQ=255)
                                                              cTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                      >  1:1742194/1‑93 (MQ=255)
                                                              cTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                      >  1:3433170/1‑93 (MQ=255)
                                                              cTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                      >  1:3633750/1‑93 (MQ=255)
                                                               tGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcc                     <  2:3780749/93‑1 (MQ=255)
                                                               tGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcc                     >  1:22599/1‑93 (MQ=255)
                                                                 ccAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAGGTAAATCCTTCTACCGGCATCCTTGCCAGCCAt                   >  1:6333073/1‑93 (MQ=255)
                                                                  cAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAtt                  >  2:5507360/1‑93 (MQ=255)
                                                                  cAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAtt                  <  2:6420901/93‑1 (MQ=255)
                                                                      aataatCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata              <  2:8401378/93‑1 (MQ=255)
                                                                      aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata              >  2:4464656/1‑93 (MQ=255)
                                                                      aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata              >  2:7633008/1‑93 (MQ=255)
                                                                      aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata              >  1:4756206/1‑93 (MQ=255)
                                                                       atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat             >  1:571226/1‑93 (MQ=255)
                                                                       atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat             <  2:7279861/93‑1 (MQ=255)
                                                                       atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat             <  1:787009/93‑1 (MQ=255)
                                                                        tcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAtt            >  2:6265987/1‑93 (MQ=255)
                                                                        tcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAtt            <  1:861744/93‑1 (MQ=255)
                                                                          atcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa          <  2:2080/93‑1 (MQ=255)
                                                                           tcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt         >  2:4031338/1‑93 (MQ=255)
                                                                           tcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt         >  2:6101429/1‑93 (MQ=255)
                                                                            ccTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATa        <  2:5807903/93‑1 (MQ=255)
                                                                            ccTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATa        <  1:6502359/93‑1 (MQ=255)
                                                                              ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATaca      <  2:4627854/93‑1 (MQ=255)
                                                                              ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATaca      >  2:6188774/1‑93 (MQ=255)
                                                                              ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATaca      >  2:5170566/1‑93 (MQ=255)
                                                                               tAGCGAAAGCTAAGGATTTTTTTTATCTGAAATACCCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATacac     <  1:5149501/93‑1 (MQ=255)
                                                                               tAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATacac     <  1:6110427/93‑1 (MQ=255)
                                                                               tAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATacac     <  2:8810202/93‑1 (MQ=255)
                                                                                 gCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACtt   >  1:4189952/1‑93 (MQ=255)
                                                                                       ||                                                                                      
AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTC  >  NC_000913/3944589‑3944763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 285 total aligned reads displayed.
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  NC_000913/3944583‑3944765
                                                                                             |                                                                                         
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAG                                                                                           <  SRR4302541.5596531/94‑1 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                      <  SRR4302541.2018734/94‑1 (MQ=60)
     TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                      >  SRR4302541.3922346/1‑94 (MQ=60)
       GTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGA                                                                                    >  SRR4302541.1760045/1‑94 (MQ=60)
       GTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGA                                                                                    >  SRR4302541.4884259/1‑94 (MQ=60)
        TTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAT                                                                                   <  SRR4302541.7798967/94‑1 (MQ=60)
         TCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATT                                                                                  >  SRR4302541.5989929/1‑94 (MQ=60)
         TCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATT                                                                                  <  SRR4302541.821846/94‑1 (MQ=60)
         TCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATT                                                                                  <  SRR4302541.6114762/94‑1 (MQ=60)
         TCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATT                                                                                  <  SRR4302541.4521059/94‑1 (MQ=60)
         TCAATTGGTAGACGCCCGGTCTCCAAAACCGGGTGCTGGGACTTCGAGTCTCTCCTCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATT                                                                                  <  SRR4302541.5064593/94‑1 (MQ=60)
          CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTT                                                                                 >  SRR4302541.1470797/1‑94 (MQ=60)
          CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTT                                                                                 <  SRR4302541.7114631/94‑1 (MQ=60)
           AATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTT                                                                                >  SRR4302541.1904063/1‑94 (MQ=60)
           AATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTT                                                                                >  SRR4302541.4330742/1‑94 (MQ=60)
            ATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTT                                                                               >  SRR4302541.515016/1‑94 (MQ=60)
            ATCGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTT                                                                               <  SRR4302541.3962378/94‑1 (MQ=60)
             TTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTT                                                                              >  SRR4302541.250322/1‑94 (MQ=60)
               GGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTT                                                                            >  SRR4302541.2393030/1‑94 (MQ=60)
               GGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTT                                                                            >  SRR4302541.982756/1‑94 (MQ=60)
                GTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTA                                                                           >  SRR4302541.7407520/1‑94 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                          <  SRR4302541.1681208/94‑1 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                          <  SRR4302541.407476/94‑1 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                          <  SRR4302541.4597361/94‑1 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                          <  SRR4302541.4653650/94‑1 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                          <  SRR4302541.3921287/94‑1 (MQ=60)
                 TAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAT                                                                          >  SRR4302541.6629068/1‑94 (MQ=60)
                  AGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATC                                                                         >  SRR4302541.6207093/1‑94 (MQ=60)
                  AGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATC                                                                         >  SRR4302541.1001973/1‑94 (MQ=60)
                   GAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCT                                                                        >  SRR4302541.5146047/1‑94 (MQ=60)
                   GAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCT                                                                        <  SRR4302541.3321774/94‑1 (MQ=60)
                    AGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTG                                                                       <  SRR4302541.7283320/94‑1 (MQ=60)
                     GCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGA                                                                      >  SRR4302541.5329998/1‑94 (MQ=60)
                      CACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAA                                                                     >  SRR4302541.3258169/1‑94 (MQ=60)
                      CACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAA                                                                     <  SRR4302541.3958401/94‑1 (MQ=60)
                      CACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAA                                                                     >  SRR4302541.4367308/1‑94 (MQ=60)
                      CACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAA                                                                     >  SRR4302541.4367081/1‑94 (MQ=60)
                      CACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAA                                                                     <  SRR4302541.4339930/94‑1 (MQ=60)
                      CACCGGTCGCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAA                                                                     <  SRR4302541.2445654/94‑1 (MQ=60)
                       ACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    >  SRR4302541.4726663/1‑94 (MQ=60)
                       ACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    >  SRR4302541.7351060/1‑94 (MQ=60)
                       ACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    >  SRR4302541.5428577/1‑94 (MQ=60)
                        CCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAT                                                                   >  SRR4302541.4936770/1‑94 (MQ=60)
                        CCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAT                                                                   <  SRR4302541.7031884/94‑1 (MQ=60)
                        CCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAT                                                                   <  SRR4302541.5173916/94‑1 (MQ=60)
                         CGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATA                                                                  <  SRR4302541.3695317/94‑1 (MQ=60)
                         CGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATA                                                                  >  SRR4302541.5568559/1‑94 (MQ=60)
                         CGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATA                                                                  <  SRR4302541.1087632/94‑1 (MQ=60)
                          GGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCTCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAA                                                                 <  SRR4302541.4511883/94‑1 (MQ=60)
                          GGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAA                                                                 >  SRR4302541.5722328/1‑94 (MQ=60)
                          GGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAA                                                                 <  SRR4302541.6596955/94‑1 (MQ=60)
                          GGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAA                                                                 >  SRR4302541.4583567/1‑94 (MQ=60)
                           GTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAC                                                                <  SRR4302541.8589651/94‑1 (MQ=60)
                           GTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAC                                                                <  SRR4302541.3034806/94‑1 (MQ=60)
                            TCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACC                                                               >  SRR4302541.1417428/1‑94 (MQ=60)
                            TCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACC                                                               <  SRR4302541.6568591/94‑1 (MQ=60)
                              tgcaacagCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                             <  SRR4302541.1219485/86‑1 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                             >  SRR4302541.7817011/1‑94 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                             <  SRR4302541.8736189/94‑1 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                             >  SRR4302541.8481770/1‑94 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                             <  SRR4302541.5895463/94‑1 (MQ=60)
                              TCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                             <  SRR4302541.5566375/94‑1 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            <  SRR4302541.1857299/94‑1 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            >  SRR4302541.2819472/1‑94 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            <  SRR4302541.7760825/94‑1 (MQ=60)
                               CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            >  SRR4302541.3385711/1‑94 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           <  SRR4302541.5916727/94‑1 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           <  SRR4302541.5077569/94‑1 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           <  SRR4302541.3464495/94‑1 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           >  SRR4302541.8592806/1‑94 (MQ=60)
                                CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           <  SRR4302541.6521636/94‑1 (MQ=60)
                                 AAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTC                                                          <  SRR4302541.2672743/94‑1 (MQ=60)
                                  AAACCGGTTGTGGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAGCCCTCTCC                                                         <  SRR4302541.2727241/94‑1 (MQ=60)
                                  AAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCC                                                         <  SRR4302541.2727242/94‑1 (MQ=60)
                                   AGCCGTGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                        <  SRR4302541.3082375/94‑1 (MQ=60)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                        >  SRR4302541.3733150/1‑94 (MQ=60)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                        <  SRR4302541.1233203/94‑1 (MQ=60)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                        <  SRR4302541.6056060/94‑1 (MQ=60)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                        <  SRR4302541.4261973/94‑1 (MQ=60)
                                   AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                        <  SRR4302541.8230970/94‑1 (MQ=60)
                                    ACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGA                                                       <  SRR4302541.4008328/94‑1 (MQ=60)
                                      CGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAG                                                     >  SRR4302541.7619361/1‑94 (MQ=60)
                                      CGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAG                                                     >  SRR4302541.111528/1‑94 (MQ=60)
                                      CGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAG                                                     >  SRR4302541.7161718/1‑94 (MQ=60)
                                      CGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAAGATTTTTTTTATCTGAAATAACCCTCTCCGAAG                                                     <  SRR4302541.7622899/94‑1 (MQ=60)
                                        GGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTA                                                   <  SRR4302541.150703/94‑1 (MQ=60)
                                         GTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAA                                                  <  SRR4302541.5648903/94‑1 (MQ=60)
                                         GTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAA                                                  <  SRR4302541.5509642/94‑1 (MQ=60)
                                          TGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAA                                                 <  SRR4302541.17064/94‑1 (MQ=60)
                                           GTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAT                                                <  SRR4302541.2767321/94‑1 (MQ=60)
                                           GTTGAGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAT                                                <  SRR4302541.241489/94‑1 (MQ=60)
                                            TTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACTCTCTCCGAAGTAAATC                                               >  SRR4302541.8802238/1‑94 (MQ=60)
                                            TTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATC                                               >  SRR4302541.4051752/1‑94 (MQ=60)
                                            TTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATC                                               <  SRR4302541.1860435/94‑1 (MQ=60)
                                            TTGGGACTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATC                                               <  SRR4302541.8184649/94‑1 (MQ=60)
                                             TGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCC                                              >  SRR4302541.2672247/1‑94 (MQ=60)
                                             TGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCC                                              <  SRR4302541.3596561/94‑1 (MQ=60)
                                             TGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCC                                              >  SRR4302541.2081906/1‑94 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             <  SRR4302541.6602070/94‑1 (MQ=60)
                                              GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             <  SRR4302541.3650166/94‑1 (MQ=60)
                                               GGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTT                                            >  SRR4302541.7719752/1‑94 (MQ=60)
                                               GGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTT                                            <  SRR4302541.7875744/94‑1 (MQ=60)
                                                GAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTC                                           <  SRR4302541.5630209/94‑1 (MQ=60)
                                                 GGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          <  SRR4302541.2984243/94‑1 (MQ=60)
                                                 AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          <  SRR4302541.8125747/94‑1 (MQ=60)
                                                 AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          <  SRR4302541.503912/94‑1 (MQ=60)
                                                 AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          <  SRR4302541.4165213/94‑1 (MQ=60)
                                                   TTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAC                                        <  SRR4302541.460994/94‑1 (MQ=60)
                                                    TCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACC                                       <  SRR4302541.2125460/94‑1 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                      >  SRR4302541.2210605/1‑94 (MQ=60)
                                                     CGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCG                                      <  SRR4302541.4183246/94‑1 (MQ=60)
                                                      GAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCTG                                     >  SRR4302541.5519891/1‑94 (MQ=60)
                                                       AGTCTCTCCGCCCCTGCGAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGC                                    <  SRR4302541.8163982/94‑1 (MQ=60)
                                                        GCCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGACAGCTAAGGATTTTTTTTATCTGCAATAACCCTCTCCGCAGTAAATCCTTCTACCGGCA                                   >  SRR4302541.1478425/1‑94 (MQ=60)
                                                         TCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCAT                                  >  SRR4302541.7298451/1‑94 (MQ=60)
                                                          CTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATC                                 >  SRR4302541.8216950/1‑94 (MQ=60)
                                                          CTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCATTCTACCGGCATC                                 >  SRR4302541.5466164/1‑94 (MQ=60)
                                                           TCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATTACCCTCTCCGAAGTAAATCCTTCTACCGGCATCC                                >  SRR4302541.1072149/1‑94 (MQ=60)
                                                           TCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCC                                <  SRR4302541.5480777/94‑1 (MQ=60)
                                                            CTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGGAATAACCCTCTCCGCAGTAAATCCTTCTACCGGCATCCT                               >  SRR4302541.6001835/1‑94 (MQ=60)
                                                            CTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCT                               <  SRR4302541.6417525/94‑1 (MQ=60)
                                                            CTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCT                               <  SRR4302541.933079/94‑1 (MQ=60)
                                                            CTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCT                               <  SRR4302541.1385442/94‑1 (MQ=60)
                                                            CTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCT                               >  SRR4302541.1541089/1‑94 (MQ=60)
                                                            CTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCT                               <  SRR4302541.6796704/94‑1 (MQ=60)
                                                             TCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTT                              <  SRR4302541.8842378/94‑1 (MQ=60)
                                                             TCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTT                              <  SRR4302541.3019884/94‑1 (MQ=60)
                                                              CCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTG                             <  SRR4302541.8562328/94‑1 (MQ=60)
                                                              CCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTG                             >  SRR4302541.6932551/1‑94 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGC                            >  SRR4302541.1728039/1‑94 (MQ=60)
                                                               CGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGC                            >  SRR4302541.2544336/1‑94 (MQ=60)
                                                                GCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCC                           <  SRR4302541.3104106/94‑1 (MQ=60)
                                                                GCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCC                           >  SRR4302541.5189379/1‑94 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTtaaattaacctcccccaaagtaaaccttctacccgcctcccttcac                          >  SRR4302541.1286174/1‑48 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTtccc                          >  SRR4302541.3512883/1‑90 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCA                          >  SRR4302541.5364828/1‑94 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCA                          >  SRR4302541.6388242/1‑94 (MQ=60)
                                                                 CCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCA                          >  SRR4302541.5917630/1‑94 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         >  SRR4302541.2311067/1‑94 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         <  SRR4302541.6848913/94‑1 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         >  SRR4302541.5728572/1‑94 (MQ=60)
                                                                  CCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         >  SRR4302541.674929/1‑94 (MQ=60)
                                                                   CCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGC                        >  SRR4302541.1759208/1‑94 (MQ=60)
                                                                   CCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGC                        >  SRR4302541.3467469/1‑94 (MQ=60)
                                                                   CCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGC                        >  SRR4302541.2297170/1‑94 (MQ=60)
                                                                   CCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGC                        >  SRR4302541.3670593/1‑94 (MQ=60)
                                                                    CTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCC                       >  SRR4302541.22783/1‑94 (MQ=60)
                                                                     TGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCA                      >  SRR4302541.291133/1‑94 (MQ=60)
                                                                     TGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCA                      >  SRR4302541.6726084/1‑94 (MQ=60)
                                                                     TGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCA                      <  SRR4302541.3963196/94‑1 (MQ=60)
                                                                      GCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATACCCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAT                     <  SRR4302541.5010444/94‑1 (MQ=60)
                                                                      GCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAGGTAAATCCTTCTACCGGCATCCTTGCCAGCCAT                     >  SRR4302541.6397821/1‑94 (MQ=60)
                                                                      GCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAT                     >  SRR4302541.1281424/1‑94 (MQ=60)
                                                                      GCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAT                     >  SRR4302541.1983838/1‑94 (MQ=60)
                                                                       CCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATT                    >  SRR4302541.244462/1‑94 (MQ=60)
                                                                       CCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATT                    <  SRR4302541.1736027/94‑1 (MQ=60)
                                                                        CAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTC                   <  SRR4302541.2134799/94‑1 (MQ=60)
                                                                        CAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTC                   >  SRR4302541.4144061/1‑94 (MQ=60)
                                                                        CAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTA                   >  SRR4302541.2447174/1‑94 (MQ=60)
                                                                        CAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCCCCATCCTTGCCAGCCATTC                   >  SRR4302541.5077133/1‑94 (MQ=60)
                                                                         AGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCA                  <  SRR4302541.8419256/94‑1 (MQ=60)
                                                                           AAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATA                >  SRR4302541.4804793/1‑94 (MQ=60)
                                                                           AAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATA                <  SRR4302541.6254255/94‑1 (MQ=60)
                                                                           AAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATA                <  SRR4302541.4604203/94‑1 (MQ=60)
                                                                            AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               <  SRR4302541.1166548/94‑1 (MQ=60)
                                                                            AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               >  SRR4302541.158816/1‑94 (MQ=60)
                                                                            AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               <  SRR4302541.1596725/94‑1 (MQ=60)
                                                                            AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               <  SRR4302541.7086269/94‑1 (MQ=60)
                                                                            AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               >  SRR4302541.576608/1‑94 (MQ=60)
                                                                             ATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATT              <  SRR4302541.794309/94‑1 (MQ=60)
                                                                              TCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTA             <  SRR4302541.869577/94‑1 (MQ=60)
                                                                              TCATCCTTAGCGAAAGCTAAGGATTTTTTTTATATGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTA             <  SRR4302541.8582026/94‑1 (MQ=60)
                                                                                ATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAT           >  SRR4302541.7000936/1‑94 (MQ=60)
                                                                                 TCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATA          >  SRR4302541.8462521/1‑94 (MQ=60)
                                                                                 TCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATA          >  SRR4302541.8249335/1‑94 (MQ=60)
                                                                                  CCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATAC         >  SRR4302541.6833175/1‑94 (MQ=60)
                                                                                   CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA        <  SRR4302541.4064850/94‑1 (MQ=60)
                                                                                   CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA        >  SRR4302541.1257815/1‑94 (MQ=60)
                                                                                   CTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACA        >  SRR4302541.7607435/1‑94 (MQ=60)
                                                                                     TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATACCCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACT      <  SRR4302541.2227473/94‑1 (MQ=60)
                                                                                     TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACT      <  SRR4302541.6172825/94‑1 (MQ=60)
                                                                                     TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACT      <  SRR4302541.7602840/94‑1 (MQ=60)
                                                                                     TAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGCCATCCTTGCCAGCCATTCATATTAATACACT      <  SRR4302541.7602841/94‑1 (MQ=60)
                                                                                      AGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTT     >  SRR4302541.8320927/1‑94 (MQ=60)
                                                                                      AGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTT     >  SRR4302541.4231542/1‑94 (MQ=60)
                                                                                      AGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTT     >  SRR4302541.7979462/1‑94 (MQ=60)
                                                                                      AGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTT     >  SRR4302541.4557504/1‑94 (MQ=60)
                                                                                       GCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAATTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTC    <  SRR4302541.5728468/94‑1 (MQ=60)
                                                                                        CGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA   <  SRR4302541.7412361/94‑1 (MQ=60)
                                                                                        CGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA   >  SRR4302541.7442241/1‑94 (MQ=60)
                                                                                         GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  SRR4302541.7745312/1‑94 (MQ=60)
                                                                                         GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  SRR4302541.3123332/1‑94 (MQ=60)
                                                                                         GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  SRR4302541.3627279/1‑94 (MQ=60)
                                                                                         GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  SRR4302541.2514629/1‑94 (MQ=60)
                                                                                         GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  SRR4302541.4809330/1‑94 (MQ=60)
                                                                                         GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  <  SRR4302541.4819239/94‑1 (MQ=60)
                                                                                         GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  SRR4302541.411283/1‑94 (MQ=60)
                                                                                         GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  <  SRR4302541.7691893/94‑1 (MQ=60)
                                                                                         GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  SRR4302541.2095303/1‑94 (MQ=60)
                                                                                         GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  <  SRR4302541.7525315/94‑1 (MQ=60)
                                                                                             |                                                                                         
GGGCGTAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  NC_000913/3944583‑3944765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑