Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,944,676 2 bp→GC intergenic (+20/+75) trpT → / ← yifDA tRNA‑Trp/transcription regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,944,6760CG100.0% 654.4 / NA 187intergenic (+20/+76)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (91/96);  total (91/96)
*NC_0009133,944,6770GC100.0% 656.1 / NA 187intergenic (+21/+75)trpT/yifDAtRNA‑Trp/transcription regulator
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (91/96);  total (91/96)

Only 100 of 189 total aligned reads displayed.

AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTC  >  NC_000913/3944589‑3944763
                                                                                       ||                                                                                      
  ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                  >  1:599779/1‑93 (MQ=255)
  ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCACCCTTAGCGAAAGCTAAGGa                                                                                  <  2:3189719/93‑1 (MQ=255)
  ttCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCCCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGa                                                                                  <  1:3771679/93‑1 (MQ=255)
   tCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAt                                                                                 >  1:4636862/1‑93 (MQ=255)
   tCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTCCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAt                                                                                 <  1:5605806/93‑1 (MQ=255)
    cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtt                                                                                <  1:3640453/93‑1 (MQ=255)
    cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtt                                                                                >  1:6190242/1‑93 (MQ=255)
    cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtt                                                                                <  2:1447855/93‑1 (MQ=255)
    cAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtt                                                                                >  1:1864087/1‑93 (MQ=255)
     aaTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAttt                                                                               <  1:1556644/93‑1 (MQ=255)
      aTTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttt                                                                              <  2:1573765/93‑1 (MQ=255)
      aTTGGTAGAGCACAGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttt                                                                              >  1:2911930/1‑93 (MQ=255)
       ttGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAttttt                                                                             <  1:3197635/93‑1 (MQ=255)
         ggTAGAGCACCGGTCTCCAAAGCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAttttttt                                                                           <  1:1837008/93‑1 (MQ=255)
          gTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAtttttttt                                                                          <  1:6095690/93‑1 (MQ=255)
           tAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTa                                                                         <  2:6157478/93‑1 (MQ=255)
            agagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAt                                                                        <  2:3140462/93‑1 (MQ=255)
            agagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTAt                                                                        <  1:1042586/93‑1 (MQ=255)
             gagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATc                                                                       <  2:487057/93‑1 (MQ=255)
             gagCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATc                                                                       <  1:728020/93‑1 (MQ=255)
              agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCt                                                                      >  2:5861705/1‑93 (MQ=255)
              agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCt                                                                      <  1:2427223/93‑1 (MQ=255)
              agCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCt                                                                      <  1:5059319/93‑1 (MQ=255)
                cACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGa                                                                    <  1:3749680/93‑1 (MQ=255)
                   cGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAt                                                                 >  2:1378649/1‑93 (MQ=255)
                   cGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAt                                                                 <  2:2463893/93‑1 (MQ=255)
                    ggTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATa                                                                >  2:4179584/1‑93 (MQ=255)
                    ggTCTCCAAAACCGGGTGTTGGGAGGTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATa                                                                >  1:3321489/1‑93 (MQ=255)
                     gTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATaa                                                               <  1:6216819/93‑1 (MQ=255)
                     gTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATaa                                                               >  2:5899719/1‑93 (MQ=255)
                     gTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATaa                                                               >  2:5594226/1‑93 (MQ=255)
                      tctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAc                                                              <  2:2502814/93‑1 (MQ=255)
                      tctcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAc                                                              <  1:3361700/93‑1 (MQ=255)
                        tcCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAccc                                                            >  1:4637862/1‑93 (MQ=255)
                         ccAAAACCGGGTGTTGTGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           <  2:5840494/93‑1 (MQ=255)
                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           <  2:2532576/93‑1 (MQ=255)
                         ccAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCct                                                           <  2:154841/93‑1 (MQ=255)
                          cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                          <  1:4350325/93‑1 (MQ=255)
                          cAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctc                                                          >  1:3019716/1‑93 (MQ=255)
                           aaaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctct                                                         <  1:5559802/93‑1 (MQ=255)
                           aaaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctct                                                         >  2:1395707/1‑93 (MQ=255)
                           aaaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCctct                                                         <  2:2993602/93‑1 (MQ=255)
                            aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCtctc                                                        >  2:2730550/1‑93 (MQ=255)
                            aaaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCtctc                                                        >  1:2087820/1‑93 (MQ=255)
                             aaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTcc                                                       >  2:4687831/1‑93 (MQ=255)
                             aaCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTcc                                                       >  2:2117569/1‑93 (MQ=255)
                              aCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCg                                                      >  1:538229/1‑93 (MQ=255)
                                cGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGaa                                                    <  2:6371036/93‑1 (MQ=255)
                                 gggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAg                                                   <  2:3668839/93‑1 (MQ=255)
                                 gggTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAg                                                   >  1:23712/1‑93 (MQ=255)
                                   gtgtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTa                                                 <  2:3547324/93‑1 (MQ=255)
                                     gtTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGCaaa                                               >  2:3533687/1‑93 (MQ=255)
                                      ttGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAt                                              <  1:335505/93‑1 (MQ=255)
                                       tGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATc                                             >  1:6335699/1‑93 (MQ=255)
                                        gggAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATcc                                            <  1:3494925/93‑1 (MQ=255)
                                          gAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCtt                                          <  1:1000099/93‑1 (MQ=255)
                                             ttCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTa                                       <  2:4563451/93‑1 (MQ=255)
                                              tCGAGTCTCTCCGCCCCTGCAAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAc                                      <  2:1322403/93‑1 (MQ=255)
                                               cGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGCTTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAcc                                     >  1:4521764/1‑93 (MQ=255)
                                               cGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTAcc                                     >  2:296736/1‑93 (MQ=255)
                                                gAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCg                                    <  2:309193/93‑1 (MQ=255)
                                                 aGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCgg                                   >  1:705684/1‑93 (MQ=255)
                                                 aGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCgg                                   >  1:3880935/1‑93 (MQ=255)
                                                  gTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGc                                  >  2:2632214/1‑93 (MQ=255)
                                                  gTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGc                                  >  2:759442/1‑93 (MQ=255)
                                                  gTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGc                                  <  1:5717067/93‑1 (MQ=255)
                                                    ctctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCAt                                <  1:3459717/93‑1 (MQ=255)
                                                     tctcCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATc                               >  1:5294336/1‑93 (MQ=255)
                                                        ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCtt                            <  1:935326/93‑1 (MQ=255)
                                                        ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCtt                            <  2:4381743/93‑1 (MQ=255)
                                                        ccGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCtt                            <  2:6310134/93‑1 (MQ=255)
                                                           ccccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcc                         >  2:3124971/1‑93 (MQ=255)
                                                           ccccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcc                         <  1:3966849/93‑1 (MQ=255)
                                                           ccccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcc                         >  1:709445/1‑93 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        <  1:6056649/93‑1 (MQ=255)
                                                            cccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgcca                        >  2:4151623/1‑93 (MQ=255)
                                                             ccTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccag                       >  2:5983652/1‑93 (MQ=255)
                                                              cTGCCAGAAATCATTCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagc                      >  2:1756936/1‑93 (MQ=255)
                                                               tGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcc                     <  2:3242812/93‑1 (MQ=255)
                                                                gCCAGAAATCATCCTTAGCGAAAGCTCAGGATTTTTTTTATCTGAAATACCCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcca                    <  1:5201270/93‑1 (MQ=255)
                                                                gCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcca                    <  1:1275434/93‑1 (MQ=255)
                                                                gCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTgccagcca                    <  2:1993663/93‑1 (MQ=255)
                                                                 ccAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAt                   >  1:1452395/1‑93 (MQ=255)
                                                                  cAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAtt                  >  2:1521337/1‑93 (MQ=255)
                                                                    gAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCa                >  2:1065163/1‑93 (MQ=255)
                                                                    gAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCa                >  1:4153511/1‑93 (MQ=255)
                                                                    gAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCa                <  2:5813867/93‑1 (MQ=255)
                                                                      aaTCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCata              <  2:5672855/93‑1 (MQ=255)
                                                                       atcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCatat             >  1:5464704/1‑93 (MQ=255)
                                                                        tcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAtt            <  2:1145588/93‑1 (MQ=255)
                                                                        tcatcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAtt            <  1:5782755/93‑1 (MQ=255)
                                                                          atcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa          >  2:5032196/1‑93 (MQ=255)
                                                                          atcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTaa          >  2:1912159/1‑93 (MQ=255)
                                                                           tcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt         <  1:875114/93‑1 (MQ=255)
                                                                           tcCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAt         <  2:599779/93‑1 (MQ=255)
                                                                             cTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATac       >  2:5403727/1‑93 (MQ=255)
                                                                              ttAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATaca      <  2:3296704/93‑1 (MQ=255)
                                                                                aGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACt    <  2:1586924/93‑1 (MQ=255)
                                                                                 gCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAATTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACtt   <  1:2345243/93‑1 (MQ=255)
                                                                                 gCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACtt   <  2:5281444/93‑1 (MQ=255)
                                                                                       ||                                                                                      
AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTC  >  NC_000913/3944589‑3944763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  NC_000913/3944588‑3944765
                                                                                        |                                                                                         
gagttcattgtGTAGAGCACCGGTCTCCAAACCCGGGTGTTGGGAGTTCGAGTCCCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                      <  SRR4302534.4696462/83‑1 (MQ=60)
TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAG                                                                                      >  SRR4302534.624014/1‑94 (MQ=60)
 AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGG                                                                                     <  SRR4302534.800030/94‑1 (MQ=60)
 AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGG                                                                                     <  SRR4302534.6471633/94‑1 (MQ=60)
 AGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGG                                                                                     <  SRR4302534.1862323/94‑1 (MQ=60)
  GTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGA                                                                                    >  SRR4302534.614401/1‑94 (MQ=60)
   TTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAT                                                                                   >  SRR4302534.4762679/1‑94 (MQ=60)
   TTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCCCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGAT                                                                                   <  SRR4302534.3872955/94‑1 (MQ=60)
    TCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATT                                                                                  >  SRR4302534.6358695/1‑94 (MQ=60)
    TCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATT                                                                                  >  SRR4302534.1912606/1‑94 (MQ=60)
    TCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTCCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATT                                                                                  <  SRR4302534.5757437/94‑1 (MQ=60)
     CAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTT                                                                                 <  SRR4302534.3738295/94‑1 (MQ=60)
      AATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTT                                                                                <  SRR4302534.1597398/94‑1 (MQ=60)
      AATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTT                                                                                >  SRR4302534.2207653/1‑94 (MQ=60)
      AATTGGTAGAGCACAGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTT                                                                                >  SRR4302534.2988366/1‑94 (MQ=60)
        TTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTT                                                                              <  SRR4302534.3282404/94‑1 (MQ=60)
          GGTAGAGCACCGGTCTCCAAAGCCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTT                                                                            <  SRR4302534.1884766/94‑1 (MQ=60)
           GTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTA                                                                           <  SRR4302534.6261451/94‑1 (MQ=60)
             AGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATC                                                                         <  SRR4302534.1069505/94‑1 (MQ=60)
              GAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCT                                                                        <  SRR4302534.746000/94‑1 (MQ=60)
               AGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTG                                                                       <  SRR4302534.5195960/94‑1 (MQ=60)
               AGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTG                                                                       <  SRR4302534.4117530/94‑1 (MQ=60)
               AGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTG                                                                       <  SRR4302534.908331/94‑1 (MQ=60)
               AGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTG                                                                       <  SRR4302534.2491761/94‑1 (MQ=60)
                 CACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAA                                                                     <  SRR4302534.3850396/94‑1 (MQ=60)
                  ACCGGTCTCCAAAACCGGGTGTTGGGAGTCCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAA                                                                    <  SRR4302534.6523475/94‑1 (MQ=60)
                   CCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAAT                                                                   <  SRR4302534.3245095/94‑1 (MQ=60)
                    GGGTCTCCAAAACCGGGTGTTGGGAGGTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATA                                                                  >  SRR4302534.3410308/1‑94 (MQ=60)
                      GTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAAC                                                                <  SRR4302534.6385717/94‑1 (MQ=60)
                       TCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACC                                                               <  SRR4302534.3451743/94‑1 (MQ=60)
                        CTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCC                                                              >  SRR4302534.1518360/1‑94 (MQ=60)
                        CTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCC                                                              >  SRR4302534.4763702/1‑94 (MQ=60)
                         CCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCT                                                             <  SRR4302534.3448003/94‑1 (MQ=60)
                          CCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTC                                                            >  SRR4302534.3098782/1‑94 (MQ=60)
                           CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           <  SRR4302534.1948117/94‑1 (MQ=60)
                           CAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCT                                                           <  SRR4302534.4467758/94‑1 (MQ=60)
                            AAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTC                                                          <  SRR4302534.5709906/94‑1 (MQ=60)
                            AAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTC                                                          >  SRR4302534.2142538/1‑94 (MQ=60)
                            AAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTCTTTTTATCTGAAATAACCCTCTC                                                          >  SRR4302534.3559799/1‑94 (MQ=60)
                             AAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCC                                                         <  SRR4302534.6005515/94‑1 (MQ=60)
                             AAACCGGGGGTTGGGAGCTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCC                                                         >  SRR4302534.6208550/1‑94 (MQ=60)
                              AACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCG                                                        >  SRR4302534.551564/1‑94 (MQ=60)
                                 CGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAG                                                     >  SRR4302534.24339/1‑94 (MQ=60)
                                  GGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGT                                                    >  SRR4302534.5389297/1‑94 (MQ=60)
                                   GGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTA                                                   <  SRR4302534.452706/94‑1 (MQ=60)
                                     TGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAA                                                 <  SRR4302534.4403147/94‑1 (MQ=60)
                                      GTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAAT                                                <  SRR4302534.3953154/94‑1 (MQ=60)
                                       TTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATC                                               >  SRR4302534.6506852/1‑94 (MQ=60)
                                       TTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATC                                               <  SRR4302534.344101/94‑1 (MQ=60)
                                        TGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCC                                              <  SRR4302534.6298972/94‑1 (MQ=60)
                                         GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             >  SRR4302534.385268/1‑94 (MQ=60)
                                         GGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCT                                             <  SRR4302534.3588905/94‑1 (MQ=60)
                                           GAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTC                                           <  SRR4302534.1025731/94‑1 (MQ=60)
                                            AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          >  SRR4302534.4742687/1‑94 (MQ=60)
                                            AGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCT                                          <  SRR4302534.6380532/94‑1 (MQ=60)
                                               TCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGCTTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACC                                       >  SRR4302534.4644251/1‑94 (MQ=60)
                                               TCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACC                                       <  SRR4302534.2426729/94‑1 (MQ=60)
                                                 GAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGG                                     >  SRR4302534.3985141/1‑94 (MQ=60)
                                                 GAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGG                                     >  SRR4302534.5111601/1‑94 (MQ=60)
                                                 GAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGG                                     >  SRR4302534.723000/1‑94 (MQ=60)
                                                  AGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGC                                    >  SRR4302534.5693836/1‑94 (MQ=60)
                                                   GTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCA                                   <  SRR4302534.5871963/94‑1 (MQ=60)
                                                    TCTCTCCGCCCCTGCCAGAAATCTTCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCAT                                  <  SRR4302534.3454208/94‑1 (MQ=60)
                                                    TCTCTCCGCCCCTGCCAGAAATCATACTTAGCGACAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCTGAAGTAAATCCTTCTACCGGCAT                                  >  SRR4302534.1127267/1‑94 (MQ=60)
                                                     CTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATC                                 <  SRR4302534.3552713/94‑1 (MQ=60)
                                                     CTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATC                                 >  SRR4302534.5437236/1‑94 (MQ=60)
                                                      TCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCC                                <  SRR4302534.582364/94‑1 (MQ=60)
                                                        CCCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTT                              <  SRR4302534.1899778/94‑1 (MQ=60)
                                                         TCGCCCCTTCCAGAAATCATCCTTATCGAAAGGTAAAGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTTAATCCTTCTACCGGCATgcctg                             >  SRR4302534.4878697/1‑89 (MQ=60)
                                                         CCGCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTG                             <  SRR4302534.959122/94‑1 (MQ=60)
                                                           GCCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCC                           >  SRR4302534.726864/1‑94 (MQ=60)
                                                            CCCCTGCCAGAAATCATCCTTAGCGAAAGCTCAGGATTTTTTTTTTCTAGAATAACCCTCTCCCAAGTCAATCCctccaacagcctccatagca                          >  SRR4302534.2984062/1‑74 (MQ=28)
                                                            CCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTTCCGGCATCCTTGCCA                          <  SRR4302534.4029504/94‑1 (MQ=60)
                                                            CCCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCA                          <  SRR4302534.4073033/94‑1 (MQ=60)
                                                             CCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         >  SRR4302534.3362689/1‑94 (MQ=60)
                                                             CCCTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAG                         <  SRR4302534.6221350/94‑1 (MQ=60)
                                                               CTGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCC                       >  SRR4302534.5733155/1‑94 (MQ=60)
                                                                TGCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCA                      >  SRR4302534.5493052/1‑94 (MQ=60)
                                                                 GCCAGAAATCATCCTTAGCGAAAGCTCAGGATTTTTTTTATCTGAAATACCCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAT                     <  SRR4302534.5341587/94‑1 (MQ=60)
                                                                 GCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAT                     >  SRR4302534.1490603/1‑94 (MQ=60)
                                                                 GCCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCAT                     <  SRR4302534.1309005/94‑1 (MQ=60)
                                                                  CCAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATT                    >  SRR4302534.3065399/1‑94 (MQ=60)
                                                                   CAGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTC                   <  SRR4302534.146899/94‑1 (MQ=60)
                                                                    AGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCA                  >  SRR4302534.4265643/1‑94 (MQ=60)
                                                                    AGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCA                  >  SRR4302534.1880627/1‑94 (MQ=60)
                                                                    AGAAATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCA                  <  SRR4302534.3774238/94‑1 (MQ=60)
                                                                       AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               >  SRR4302534.796584/1‑94 (MQ=60)
                                                                       AATCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATAT               >  SRR4302534.5612009/1‑94 (MQ=60)
                                                                         TCATCCTTAGCGAAAGCTAAGGGCTTTTTTTATCTGAAATAACCCTCTCCGCAGTCAATCCTTCTAACGGCATACTAGCCAGCCAATCCTATTA             >  SRR4302534.3496135/1‑94 (MQ=34)
                                                                         TCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCCTATTA             >  SRR4302534.2738472/1‑94 (MQ=60)
                                                                         TCATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTA             <  SRR4302534.5939619/94‑1 (MQ=60)
                                                                           ATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAT           >  SRR4302534.3421425/1‑94 (MQ=60)
                                                                           ATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAT           >  SRR4302534.725413/1‑94 (MQ=60)
                                                                           ATCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAAT           >  SRR4302534.5587938/1‑94 (MQ=60)
                                                                            TCCTTAGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATA          <  SRR4302534.897253/94‑1 (MQ=60)
                                                                                 AGCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTC     >  SRR4302534.5318130/1‑94 (MQ=60)
                                                                                  GCGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAATTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTC    <  SRR4302534.2407477/94‑1 (MQ=60)
                                                                                   CGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA   >  SRR4302534.4694250/1‑94 (MQ=60)
                                                                                   CGAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCA   >  SRR4302534.2894928/1‑94 (MQ=60)
                                                                                    GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  <  SRR4302534.1548227/94‑1 (MQ=60)
                                                                                    GAAAGCTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  <  SRR4302534.1925467/94‑1 (MQ=60)
                                                                                        |                                                                                         
TAGTTCAATTGGTAGAGCACCGGTCTCCAAAACCGGGTGTTGGGAGTTCGAGTCTCTCCGCCCCTGCCAGAAATCATCCTTAGCGAAACGTAAGGATTTTTTTTATCTGAAATAACCCTCTCCGAAGTAAATCCTTCTACCGGCATCCTTGCCAGCCATTCATATTAATACACTTCAT  >  NC_000913/3944588‑3944765

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑