Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,557 2 bp→GC coding (451‑452/930 nt) ilvE → branched‑chain amino‑acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,5570CG99.8% 1671.0 / NA 482R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (241/240);  minor base T (0/1);  total (241/241)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,950,5580GC100.0% 1649.1 / NA 483R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (241/242);  total (241/242)

Only 100 of 504 total aligned reads displayed.

ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950470‑3950644
                                                                                       ||                                                                                      
  tggtgccTTCCCGTGGGGAGCGTATCTGGGCGTAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCaaa                                                                                  <  1:7043014/90‑1 (MQ=255)
  cGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCaaa                                                                                  <  2:2811398/93‑1 (MQ=255)
  cGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCaaa                                                                                  <  2:8731367/93‑1 (MQ=255)
  cGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCaaa                                                                                  <  2:4365532/93‑1 (MQ=255)
   gctgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAac                                                                                 <  1:4211710/93‑1 (MQ=255)
     tgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac                                                                               >  1:7434534/1‑93 (MQ=255)
     tgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac                                                                               >  2:3936889/1‑93 (MQ=255)
     tgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGGTTCCTCCTGGAACCGCGCAGCACCAAacac                                                                               >  2:5130603/1‑93 (MQ=255)
      gctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAcc                                                                              <  1:1828674/93‑1 (MQ=255)
       ctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCa                                                                             <  2:2891444/93‑1 (MQ=255)
       ctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCa                                                                             >  1:2977075/1‑93 (MQ=255)
        tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt                                                                            <  2:781547/93‑1 (MQ=255)
        tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAt                                                                            >  2:1754477/1‑93 (MQ=255)
         ttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATc                                                                           >  2:5735189/1‑93 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                          <  2:3768748/93‑1 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                          <  2:7548545/93‑1 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                          <  1:7624332/93‑1 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                          >  1:6056894/1‑93 (MQ=255)
             cGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGa                                                                       <  2:3632159/93‑1 (MQ=255)
             cGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGa                                                                       <  2:3402567/93‑1 (MQ=255)
              gTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAc                                                                      >  1:2096935/1‑93 (MQ=255)
                 gggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggc                                                                   >  1:455510/1‑93 (MQ=255)
                   gAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggcgg                                                                 <  2:1363665/93‑1 (MQ=255)
                    aGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCTGAcggcgcc                                                                >  1:6453753/1‑91 (MQ=255)
                     gCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa                                                               >  1:4715626/1‑93 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                            <  2:3171445/93‑1 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                            <  2:2652571/93‑1 (MQ=255)
                         aTCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAAACCATCCCGACGGCGGCAAAAg                                                           >  2:2086863/1‑93 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                          <  2:6374999/93‑1 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                          >  2:8212339/1‑93 (MQ=255)
                            tGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg                                                        >  1:5582186/1‑93 (MQ=255)
                            tGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg                                                        <  2:7288970/93‑1 (MQ=255)
                             gggCGCAGAAGCGCTGGAGCATGGGATCGATGCGCTGGTTTCCTCATTGAACCGCGCAGCACCACACAACATCCCGGCGGCGGCAAAAGCCgg                                                       >  2:5279549/1‑93 (MQ=255)
                             gggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCgg                                                       <  2:5500881/93‑1 (MQ=255)
                              ggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggg                                                      >  1:1700612/1‑92 (MQ=255)
                                 gcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtggt                                                   <  2:480225/93‑1 (MQ=255)
                                 gcAGAAGCGCTGGAGCAGGGGAGCGATTCGATGGTTTCCTCCTGGAACCGCGCAGGACCAAACACCATCCCGACGGCGGCAAAAGCCggtggt                                                   >  1:5447199/1‑93 (MQ=255)
                                 gaaGAAGCGCTAGAGCAGGTGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtggt                                                   <  2:2328177/91‑1 (MQ=255)
                                  cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTa                                                  >  2:6017589/1‑93 (MQ=255)
                                  cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTa                                                  <  1:2807777/93‑1 (MQ=255)
                                   aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTaa                                                 <  2:1678450/93‑1 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGCCGGCGGCAACAGCCGGTGGTaaa                                                >  1:229667/1‑92 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                                >  2:2528794/1‑93 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                                >  1:391583/1‑93 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                                >  2:1006858/1‑93 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                                >  2:7019669/1‑93 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                                <  1:8366379/93‑1 (MQ=255)
                                         gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACct                                           <  1:122376/93‑1 (MQ=255)
                                         gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACct                                           <  1:7534224/93‑1 (MQ=255)
                                         gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACct                                           >  1:4955878/1‑93 (MQ=255)
                                         gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACct                                           <  1:5962533/93‑1 (MQ=255)
                                           tGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctct                                         >  2:125019/1‑93 (MQ=255)
                                           tGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctct                                         >  1:4308346/1‑93 (MQ=255)
                                           tGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctct                                         <  2:7980464/93‑1 (MQ=255)
                                              aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCtt                                      >  1:1913542/1‑93 (MQ=255)
                                               gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc                                     >  2:1517002/1‑93 (MQ=255)
                                                  ggggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCct                                  >  1:8661100/1‑93 (MQ=255)
                                                  ggggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCct                                  <  1:6283071/93‑1 (MQ=255)
                                                   gggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTCACTACCTCTCTTCCctg                                 >  1:263019/1‑93 (MQ=255)
                                                     gATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgct                               <  1:4084431/93‑1 (MQ=255)
                                                      aTCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgctg                              >  1:6729908/1‑93 (MQ=255)
                                                      aTCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgctg                              >  1:3049507/1‑93 (MQ=255)
                                                       tCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTGCCTCTCTTCCCTGCtgg                             <  2:6070850/93‑1 (MQ=255)
                                                       tCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtgg                             <  1:8088121/93‑1 (MQ=255)
                                                       tCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtgg                             <  1:3669263/93‑1 (MQ=255)
                                                        cgatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggt                            <  2:7723947/93‑1 (MQ=255)
                                                         gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           <  1:4836331/93‑1 (MQ=255)
                                                         gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           >  1:2487369/1‑93 (MQ=255)
                                                         gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           >  1:6830746/1‑93 (MQ=255)
                                                         gatgcgatgGTTTACTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           >  1:321799/1‑93 (MQ=255)
                                                           tgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTggg                         <  2:710205/93‑1 (MQ=255)
                                                            gcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt                        <  2:1124445/93‑1 (MQ=255)
                                                            gcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt                        <  2:4873233/93‑1 (MQ=255)
                                                            gcgatcGTTTCCTCCTGGAACCGCGCAGCACCAAACCCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt                        <  1:728448/93‑1 (MQ=255)
                                                             cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                       <  1:3782401/93‑1 (MQ=255)
                                                             cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                       <  1:7788450/93‑1 (MQ=255)
                                                              gatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAGCTACCTCTCTTCCCTGCTGGTGGGTAg                      >  1:1573471/1‑93 (MQ=255)
                                                              gatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAg                      <  2:5375302/93‑1 (MQ=255)
                                                               atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                     <  2:3699444/93‑1 (MQ=255)
                                                                  gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                  <  2:945626/93‑1 (MQ=255)
                                                                  gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                  <  2:1610623/93‑1 (MQ=255)
                                                                  gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                  <  1:7913998/93‑1 (MQ=255)
                                                                  gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                  <  1:5218038/93‑1 (MQ=255)
                                                                   tttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAg                 <  1:1327086/93‑1 (MQ=255)
                                                                    ttCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGCCGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgc                <  1:1996946/93‑1 (MQ=255)
                                                                       ctcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgcg             >  2:6431352/1‑93 (MQ=255)
                                                                        ccctGGACCCGCGCAGCACCAAACCCCACCCCGACGGCGGCAAAAGCCGGTGGTAACTCCCTCTCTCCCCTGCTGGTGGGTAGCGAAgcgcgc            <  2:6238350/92‑1 (MQ=255)
                                                                        ccctGGACCCGCGCAGCACCAAACACCATCCCGACGGCGGAAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgcgc            <  1:583856/92‑1 (MQ=255)
                                                                         cctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgcc           <  2:2227713/93‑1 (MQ=255)
                                                                         cctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgcc           >  1:942119/1‑93 (MQ=255)
                                                                           tGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgccgc         >  1:5110104/1‑93 (MQ=255)
                                                                            ggAACCGCGCAGCACCAAACACCATCCCGATGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCgccgcc        <  1:349582/93‑1 (MQ=255)
                                                                            ggAACCGCCCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGGGGTCACTACCTCTCTTCCCTGCTGGTTGGTAGCGAAGCGCgccgcc        >  2:414230/1‑93 (MQ=255)
                                                                             gAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa       >  1:5710869/1‑93 (MQ=255)
                                                                              aaCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAc      <  1:1014440/93‑1 (MQ=255)
                                                                               aCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACg     >  2:6847049/1‑93 (MQ=255)
                                                                               aCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACg     >  2:8412278/1‑93 (MQ=255)
                                                                                ccGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACgg    >  2:850299/1‑93 (MQ=255)
                                                                                ccGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACgg    >  2:196063/1‑93 (MQ=255)
                                                                                 cgcgCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCCGGTGGGTAGCGAAGCGCGCCGCCACGGt   >  1:4125783/1‑93 (MQ=255)
                                                                                       ||                                                                                      
ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950470‑3950644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 264 total aligned reads displayed.
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  NC_000913/3950464‑3950646
                                                                                             |                                                                                         
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAG                                                                                           >  SRR4302541.2011702/1‑94 (MQ=60)
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAG                                                                                           <  SRR4302541.663931/94‑1 (MQ=60)
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAG                                                                                           <  SRR4302541.6547419/94‑1 (MQ=60)
GTGATTATCGCTGCTTTCCCGCGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAG                                                                                           <  SRR4302541.5506635/94‑1 (MQ=60)
     TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCA                                                                                      >  SRR4302541.3477364/1‑94 (MQ=60)
     TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCA                                                                                      <  SRR4302541.4105349/94‑1 (MQ=60)
     TATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCA                                                                                      >  SRR4302541.6903214/1‑94 (MQ=60)
      ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAA                                                                                     <  SRR4302541.8807040/94‑1 (MQ=60)
       TCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAA                                                                                    <  SRR4302541.2227197/94‑1 (MQ=60)
        tggtgccTTCCCGTGGGGAGCGTATCTGGGCGTAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   <  SRR4302541.7117902/87‑1 (MQ=60)
        CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   <  SRR4302541.4777378/94‑1 (MQ=60)
        CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   <  SRR4302541.8236264/94‑1 (MQ=60)
        CGCTGCTTTCCCGTGGGGAGCGTATCGCGCCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   <  SRR4302541.4777379/94‑1 (MQ=60)
         GCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACA                                                                                  >  SRR4302541.3040311/1‑94 (MQ=60)
         GCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACA                                                                                  >  SRR4302541.934657/1‑94 (MQ=60)
         GCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACA                                                                                  >  SRR4302541.3040310/1‑94 (MQ=60)
         GCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACCACCAAACA                                                                                  >  SRR4302541.6425395/1‑94 (MQ=60)
          CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                                 >  SRR4302541.7522128/1‑94 (MQ=60)
          CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                                 >  SRR4302541.7514012/1‑94 (MQ=60)
          CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                                 >  SRR4302541.5643888/1‑94 (MQ=60)
           TGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACC                                                                                >  SRR4302541.3577443/1‑94 (MQ=60)
            GCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCA                                                                               <  SRR4302541.1846413/94‑1 (MQ=60)
             CTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCAT                                                                              <  SRR4302541.5214898/94‑1 (MQ=60)
              TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                             <  SRR4302541.4135179/94‑1 (MQ=60)
               TTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCC                                                                            <  SRR4302541.7617368/94‑1 (MQ=60)
               TTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCC                                                                            >  SRR4302541.6118787/1‑94 (MQ=60)
               TTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCC                                                                            <  SRR4302541.1092270/94‑1 (MQ=60)
               TTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCC                                                                            >  SRR4302541.2950848/1‑94 (MQ=60)
                TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           <  SRR4302541.1750894/94‑1 (MQ=60)
                TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           <  SRR4302541.8796172/94‑1 (MQ=60)
                TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           <  SRR4302541.7705720/94‑1 (MQ=60)
                TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           <  SRR4302541.5870847/94‑1 (MQ=60)
                TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           >  SRR4302541.2933853/1‑94 (MQ=60)
                GCCCGTGGGGAGCGGATCGGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           <  SRR4302541.7592092/94‑1 (MQ=60)
                 CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                          <  SRR4302541.991407/94‑1 (MQ=60)
                 CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                          <  SRR4302541.2801009/94‑1 (MQ=60)
                  CCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGA                                                                         <  SRR4302541.2732729/94‑1 (MQ=60)
                   CGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                        >  SRR4302541.2117258/1‑94 (MQ=60)
                   CGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAC                                                                        >  SRR4302541.6825340/1‑94 (MQ=60)
                    CTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                       <  SRR4302541.3900240/94‑1 (MQ=60)
                     TGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGG                                                                      >  SRR4302541.3023000/1‑94 (MQ=60)
                      GGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGC                                                                     >  SRR4302541.459850/1‑94 (MQ=60)
                      GGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGC                                                                     <  SRR4302541.2016628/94‑1 (MQ=60)
                      GGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGC                                                                     >  SRR4302541.5250009/1‑94 (MQ=60)
                       GGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCG                                                                    >  SRR4302541.2005689/1‑94 (MQ=60)
                       GGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCG                                                                    <  SRR4302541.6319524/94‑1 (MQ=60)
                        GGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGG                                                                   >  SRR4302541.2618938/1‑94 (MQ=60)
                         GAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCTGACGGCGCC                                                                  >  SRR4302541.6519525/1‑94 (MQ=60)
                         GAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC                                                                  >  SRR4302541.7730572/1‑94 (MQ=60)
                         GAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC                                                                  >  SRR4302541.6519524/1‑94 (MQ=60)
                         GAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC                                                                  >  SRR4302541.4053331/1‑94 (MQ=60)
                           GCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAA                                                                >  SRR4302541.7856944/1‑94 (MQ=60)
                           GCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAA                                                                <  SRR4302541.3792957/94‑1 (MQ=60)
                            CGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAA                                                               >  SRR4302541.3759524/1‑94 (MQ=60)
                             GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAG                                                              >  SRR4302541.2367683/1‑94 (MQ=60)
                             GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                              >  SRR4302541.2367682/1‑94 (MQ=60)
                             GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCCGCAAAA                                                              >  SRR4302541.794023/1‑94 (MQ=60)
                              TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                             <  SRR4302541.8004236/94‑1 (MQ=60)
                              TATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAG                                                             >  SRR4302541.6050986/1‑94 (MQ=60)
                               ATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACGCCATCCCGACGGCGGCAAAAGC                                                            >  SRR4302541.3884166/1‑94 (MQ=60)
                               ATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGC                                                            >  SRR4302541.1723655/1‑94 (MQ=60)
                               ATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGC                                                            >  SRR4302541.7274766/1‑94 (MQ=60)
                                TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                           <  SRR4302541.7626386/94‑1 (MQ=60)
                                TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                           <  SRR4302541.7216466/94‑1 (MQ=60)
                                TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                           <  SRR4302541.3148489/94‑1 (MQ=60)
                                 CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                          >  SRR4302541.1548338/1‑94 (MQ=60)
                                 CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                          <  SRR4302541.5040969/94‑1 (MQ=60)
                                 CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                          >  SRR4302541.3981663/1‑94 (MQ=60)
                                 CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                          >  SRR4302541.920422/1‑94 (MQ=60)
                                 CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                          >  SRR4302541.5639328/1‑94 (MQ=60)
                                  TGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCTGCAAAAGCCGG                                                         <  SRR4302541.4536770/94‑1 (MQ=60)
                                     GCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGG                                                      <  SRR4302541.6235111/94‑1 (MQ=60)
                                      CGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGT                                                     >  SRR4302541.5503093/1‑94 (MQ=60)
                                      CGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGT                                                     >  SRR4302541.1189360/1‑94 (MQ=60)
                                      CGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTGCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGT                                                     <  SRR4302541.6498150/94‑1 (MQ=60)
                                       GCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTA                                                    <  SRR4302541.2297378/94‑1 (MQ=60)
                                        CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAA                                                   >  SRR4302541.7973495/1‑94 (MQ=60)
                                        CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAA                                                   <  SRR4302541.2836170/94‑1 (MQ=60)
                                        CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAA                                                   >  SRR4302541.415696/1‑94 (MQ=60)
                                        CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGAAAAAGCCGGTGGTAA                                                   >  SRR4302541.2428367/1‑94 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCGCCAAACACCATCCCGACGGAGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302541.8665981/1‑94 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302541.5703651/1‑94 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302541.5970310/1‑94 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302541.2825871/1‑94 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302541.395209/1‑94 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  <  SRR4302541.611982/94‑1 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302541.1945891/1‑94 (MQ=60)
                                          GAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACT                                                 <  SRR4302541.7913504/94‑1 (MQ=60)
                                          GAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACT                                                 <  SRR4302541.5432470/94‑1 (MQ=60)
                                          GAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACT                                                 >  SRR4302541.230367/1‑94 (MQ=60)
                                          GAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACT                                                 <  SRR4302541.4945737/94‑1 (MQ=60)
                                           AAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTC                                                >  SRR4302541.5451457/1‑94 (MQ=60)
                                            AGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAC                                               <  SRR4302541.3994774/94‑1 (MQ=60)
                                            AGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGACGGTGGTAACTAC                                               >  SRR4302541.1471592/1‑94 (MQ=60)
                                              CGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCT                                             <  SRR4302541.8449869/94‑1 (MQ=60)
                                              CGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCT                                             >  SRR4302541.5006561/1‑94 (MQ=60)
                                               GCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTC                                            <  SRR4302541.4707194/94‑1 (MQ=60)
                                               GCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTC                                            <  SRR4302541.123350/94‑1 (MQ=60)
                                               GCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTC                                            <  SRR4302541.7614750/94‑1 (MQ=60)
                                                CTGGAGCAGGGGATCGATGCGATTGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCT                                           >  SRR4302541.3138105/1‑94 (MQ=60)
                                                CTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCT                                           >  SRR4302541.4351374/1‑94 (MQ=60)
                                                CTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCT                                           <  SRR4302541.4958667/94‑1 (MQ=60)
                                                 TGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTC                                          <  SRR4302541.7518399/94‑1 (MQ=60)
                                                 TGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTC                                          >  SRR4302541.4294596/1‑94 (MQ=60)
                                                 TGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTC                                          >  SRR4302541.4740431/1‑94 (MQ=60)
                                                  GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                         <  SRR4302541.5698117/94‑1 (MQ=60)
                                                  GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                         <  SRR4302541.1693712/94‑1 (MQ=60)
                                                  GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                         <  SRR4302541.4265873/94‑1 (MQ=60)
                                                   GAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTT                                        >  SRR4302541.1931942/1‑94 (MQ=60)
                                                    AGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTC                                       >  SRR4302541.960507/1‑94 (MQ=60)
                                                     GCAGGGGGTCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCC                                      <  SRR4302541.7665157/94‑1 (MQ=60)
                                                     GCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCC                                      <  SRR4302541.5112028/94‑1 (MQ=60)
                                                     GCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCC                                      <  SRR4302541.5865314/94‑1 (MQ=60)
                                                      GAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                     <  SRR4302541.5947252/94‑1 (MQ=60)
                                                      CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                     >  SRR4302541.169428/1‑94 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    <  SRR4302541.8745846/94‑1 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    <  SRR4302541.5447506/94‑1 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    >  SRR4302541.8753800/1‑94 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    <  SRR4302541.2589947/94‑1 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    >  SRR4302541.8038500/1‑94 (MQ=60)
                                                        GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGCCGGCGGCCAAAGCCGGTGGTAACTACCTCTCTTCCCTG                                   >  SRR4302541.265169/1‑94 (MQ=60)
                                                        GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTCACTACCTCTCTTCCCTG                                   >  SRR4302541.265171/1‑94 (MQ=60)
                                                        GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTG                                   <  SRR4302541.7442584/94‑1 (MQ=60)
                                                        GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTG                                   <  SRR4302541.6347252/94‑1 (MQ=60)
                                                         GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                  >  SRR4302541.1060242/1‑94 (MQ=60)
                                                         GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                  >  SRR4302541.4670134/1‑94 (MQ=60)
                                                          GGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCT                                 <  SRR4302541.2080278/94‑1 (MQ=60)
                                                           GATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTG                                >  SRR4302541.6801738/1‑94 (MQ=60)
                                                           GATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTG                                <  SRR4302541.1339153/94‑1 (MQ=60)
                                                           GATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTG                                <  SRR4302541.4124743/94‑1 (MQ=60)
                                                            ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                               >  SRR4302541.6095770/1‑94 (MQ=60)
                                                            ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                               >  SRR4302541.1760817/1‑94 (MQ=60)
                                                             TCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGT                              <  SRR4302541.1930104/94‑1 (MQ=60)
                                                             TCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGT                              <  SRR4302541.8176797/94‑1 (MQ=60)
                                                             TCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGT                              <  SRR4302541.4323053/94‑1 (MQ=60)
                                                             TCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGT                              <  SRR4302541.3706470/94‑1 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302541.4187277/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302541.6903832/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             <  SRR4302541.4384012/94‑1 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302541.1240584/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302541.5268294/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302541.3555451/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302541.2512385/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTCCTACTGGAACCGCGCAGCACCAAACACAATCCCGACGGCGGCACAAGCCGGTGGGAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302541.1240583/1‑94 (MQ=60)
                                                              CGATGCGATGGTTTACTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTG                             >  SRR4302541.324669/1‑94 (MQ=60)
                                                               GATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGG                            <  SRR4302541.4885634/94‑1 (MQ=60)
                                                               GATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGG                            >  SRR4302541.7459264/1‑94 (MQ=60)
                                                               GATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGG                            <  SRR4302541.3777939/94‑1 (MQ=60)
                                                               GATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGG                            <  SRR4302541.4177861/94‑1 (MQ=60)
                                                                 TGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGT                          <  SRR4302541.8427094/94‑1 (MQ=60)
                                                                  GCGATGTTTTCCGCCTGGAACCGCGCAGCGCCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTA                         <  SRR4302541.2085330/94‑1 (MQ=60)
                                                                  GCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTA                         <  SRR4302541.6938317/94‑1 (MQ=60)
                                                                   CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAGCTACCTCTCTTCCCTGCTGGTGGGTAG                        >  SRR4302541.1589209/1‑94 (MQ=60)
                                                                     ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                      >  SRR4302541.464415/1‑94 (MQ=60)
                                                                     ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                      >  SRR4302541.1414736/1‑94 (MQ=60)
                                                                      TGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGA                     >  SRR4302541.3578222/1‑94 (MQ=60)
                                                                       GGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAA                    <  SRR4302541.6873253/94‑1 (MQ=60)
                                                                        GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                   <  SRR4302541.8001087/94‑1 (MQ=60)
                                                                        GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                   <  SRR4302541.6057979/94‑1 (MQ=60)
                                                                        GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                   <  SRR4302541.5271811/94‑1 (MQ=60)
                                                                         TTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGC                  <  SRR4302541.1340133/94‑1 (MQ=60)
                                                                         TTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGC                  <  SRR4302541.7034275/94‑1 (MQ=60)
                                                                         TTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGC                  <  SRR4302541.3186574/94‑1 (MQ=60)
                                                                         TTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGC                  <  SRR4302541.3275095/94‑1 (MQ=60)
                                                                         TTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGC                  <  SRR4302541.5015774/94‑1 (MQ=60)
                                                                         TTGCCTCCTGGAACCTCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGC                  <  SRR4302541.1945040/94‑1 (MQ=60)
                                                                         GTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGC                  <  SRR4302541.2915871/94‑1 (MQ=60)
                                                                          TTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGCCGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCG                 <  SRR4302541.2016273/94‑1 (MQ=60)
                                                                          TTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCG                 >  SRR4302541.3397316/1‑94 (MQ=60)
                                                                           TCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC                >  SRR4302541.6700169/1‑94 (MQ=60)
                                                                            CCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGCGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCG               >  SRR4302541.7384850/1‑94 (MQ=60)
                                                                             CTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGC              <  SRR4302541.6678234/94‑1 (MQ=60)
                                                                             CTCCTGGAACCGCGCAGCACCAAACACCATCACGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGC              >  SRR4302541.6001596/1‑94 (MQ=60)
                                                                              TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC             <  SRR4302541.5808803/94‑1 (MQ=60)
                                                                              TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC             >  SRR4302541.5506634/1‑94 (MQ=60)
                                                                              TCCTGGAACCGCGCAGCACCAAACACCACCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC             <  SRR4302541.7787154/94‑1 (MQ=60)
                                                                              CCCTGGACCCGCGCAGCACCAAACACCATCCCGACGGCGGAAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC             <  SRR4302541.589355/94‑1 (MQ=60)
                                                                              CCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC             <  SRR4302541.7318395/94‑1 (MQ=60)
                                                                               CCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCG            >  SRR4302541.904451/1‑94 (MQ=60)
                                                                               CCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCG            <  SRR4302541.8166271/94‑1 (MQ=60)
                                                                                CTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGC           >  SRR4302541.3890362/1‑94 (MQ=60)
                                                                                CTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGC           >  SRR4302541.4933046/1‑94 (MQ=60)
                                                                                CTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGC           >  SRR4302541.5162590/1‑94 (MQ=60)
                                                                                 TGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCC          >  SRR4302541.4364292/1‑94 (MQ=60)
                                                                                  GGAACCGCGCAGCACCAAACACCATCCCGATGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCA         <  SRR4302541.352724/94‑1 (MQ=60)
                                                                                  GGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCA         >  SRR4302541.5769103/1‑94 (MQ=60)
                                                                                  GGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCA         >  SRR4302541.4189455/1‑94 (MQ=60)
                                                                                   GAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAC        <  SRR4302541.7939650/94‑1 (MQ=60)
                                                                                    AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG       <  SRR4302541.4161000/94‑1 (MQ=60)
                                                                                    AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG       >  SRR4302541.4796018/1‑94 (MQ=60)
                                                                                    AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG       >  SRR4302541.5592025/1‑94 (MQ=60)
                                                                                    AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG       <  SRR4302541.962759/94‑1 (MQ=60)
                                                                                    AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACG       <  SRR4302541.1023820/94‑1 (MQ=60)
                                                                                     ACCGCGCAGCACCAAACACCATCCCGATGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACCG      >  SRR4302541.705157/1‑94 (MQ=60)
                                                                                     ACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGG      >  SRR4302541.3072389/1‑94 (MQ=60)
                                                                                      CCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCCGGTGGGTAGCGAAGCGCGCCGCCACGGT     >  SRR4302541.4166536/1‑94 (MQ=60)
                                                                                       CGCGCAGCACCAAACACCATCCCGGCGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT    <  SRR4302541.8621308/94‑1 (MQ=60)
                                                                                       CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT    <  SRR4302541.1914783/94‑1 (MQ=60)
                                                                                       CGCGCAGCACAAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT    <  SRR4302541.1612681/94‑1 (MQ=60)
                                                                                         CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  SRR4302541.827191/1‑94 (MQ=60)
                                                                                             |                                                                                         
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  NC_000913/3950464‑3950646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑