Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,950,557 2 bp→GC coding (451‑452/930 nt) ilvE → branched‑chain amino‑acid aminotransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,950,5570CG100.0% 787.3 / NA 224R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (119/105);  total (119/105)
*NC_0009133,950,5580GC99.6% 770.6 / ‑4.8 224R151A (CGA→GCA) ilvEbranched‑chain amino‑acid aminotransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (118/105);  minor base A (1/0);  total (119/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.11e-01

Only 100 of 230 total aligned reads displayed.

ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950470‑3950644
                                                                                       ||                                                                                      
  cGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCaaa                                                                                  >  1:3633856/1‑93 (MQ=255)
  cGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCaaa                                                                                  >  2:5192662/1‑93 (MQ=255)
  cGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCaaa                                                                                  >  2:1448955/1‑93 (MQ=255)
    ctgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAaca                                                                                <  2:6094707/93‑1 (MQ=255)
     tgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac                                                                               >  1:3524815/1‑93 (MQ=255)
     tgctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGCATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAacac                                                                               >  1:3294317/1‑93 (MQ=255)
      gctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAcc                                                                              >  2:5222431/1‑93 (MQ=255)
      gctTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAcc                                                                              <  2:4245357/93‑1 (MQ=255)
        tttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCTAACACCAt                                                                            <  1:6301369/93‑1 (MQ=255)
         ttCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATc                                                                           >  1:1395964/1‑93 (MQ=255)
         tgcccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATc                                                                           <  1:1726677/91‑1 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                          <  1:1936268/93‑1 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                          <  2:4964448/93‑1 (MQ=255)
          tCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                          <  2:2512970/93‑1 (MQ=255)
          tCCCGTGGGGAGCGTATATGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATcc                                                                          <  2:2106896/93‑1 (MQ=255)
           cccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATccc                                                                         <  2:5930105/93‑1 (MQ=255)
           cccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATccc                                                                         >  1:3055688/1‑93 (MQ=255)
            ccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg                                                                        >  2:3193968/1‑93 (MQ=255)
            ccGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCg                                                                        >  2:3079189/1‑93 (MQ=255)
                ggggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcgg                                                                    >  2:4295440/1‑93 (MQ=255)
                ggggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACAATCCCGAcgg                                                                    >  1:2133951/1‑93 (MQ=255)
                 gggAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggc                                                                   <  2:3659942/93‑1 (MQ=255)
                   gAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggcgg                                                                 >  1:1003388/1‑93 (MQ=255)
                   gAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGAcggcgg                                                                 <  1:4198389/93‑1 (MQ=255)
                    aGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACggcggc                                                                <  2:1296510/93‑1 (MQ=255)
                     gCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCa                                                               >  1:2061627/1‑93 (MQ=255)
                      cGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaa                                                              >  2:1250424/1‑93 (MQ=255)
                       gTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaa                                                             >  2:2036993/1‑93 (MQ=255)
                        tATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCaaaa                                                            <  2:4117120/93‑1 (MQ=255)
                         aTCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAg                                                           <  1:567999/93‑1 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                          <  2:3452883/93‑1 (MQ=255)
                          tCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGc                                                          >  1:5493320/1‑93 (MQ=255)
                           cTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGcc                                                         >  2:6248133/1‑93 (MQ=255)
                            tgcgcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg                                                        <  1:1522660/90‑1 (MQ=255)
                            tGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCg                                                        >  1:2940351/1‑93 (MQ=255)
                             gggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAgaagg                                                       >  1:272248/1‑89 (MQ=255)
                             gggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCgg                                                       <  2:5346822/93‑1 (MQ=255)
                              ggCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggt                                                      >  2:5635243/1‑93 (MQ=255)
                               gcgcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtg                                                     >  1:4140237/1‑93 (MQ=255)
                                 gcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtggt                                                   <  2:5438991/93‑1 (MQ=255)
                                 gcAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtggt                                                   <  2:6162756/93‑1 (MQ=255)
                                 gcAGAAGCGCGGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtggt                                                   <  1:542803/93‑1 (MQ=255)
                                  cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTa                                                  >  1:2430423/1‑93 (MQ=255)
                                  cAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTa                                                  <  2:5551852/93‑1 (MQ=255)
                                   aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTaa                                                 >  2:4600849/1‑93 (MQ=255)
                                   aGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTaa                                                 <  2:3781258/93‑1 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCggtgggcac                                                >  1:753208/1‑89 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                                >  1:2687276/1‑93 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                                <  1:4979538/93‑1 (MQ=255)
                                    gAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAc                                                >  2:4310482/1‑93 (MQ=255)
                                     aaGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACt                                               <  1:3828814/93‑1 (MQ=255)
                                       gcgcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTAc                                             >  2:86130/1‑93 (MQ=255)
                                         gcTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACct                                           <  1:1193740/93‑1 (MQ=255)
                                          cTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctc                                          <  2:269399/93‑1 (MQ=255)
                                          cTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctc                                          <  2:5354881/93‑1 (MQ=255)
                                            ggAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctc                                        <  2:3621276/93‑1 (MQ=255)
                                             gAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctct                                       <  2:3961092/93‑1 (MQ=255)
                                             gAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACctctct                                       <  2:3406196/93‑1 (MQ=255)
                                              aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCtt                                      <  1:4960332/93‑1 (MQ=255)
                                              aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCtt                                      <  2:4903226/93‑1 (MQ=255)
                                              aGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCtt                                      >  2:5486112/1‑93 (MQ=255)
                                               gCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTc                                     >  2:4043178/1‑93 (MQ=255)
                                                cAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTcc                                    >  1:1618531/1‑93 (MQ=255)
                                                cAGGGGATCGATGCGATGGTTTCCTCCTCGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTcc                                    >  1:5849963/1‑93 (MQ=255)
                                                 aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTccc                                   >  1:823895/1‑93 (MQ=255)
                                                 aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTccc                                   <  1:2476803/93‑1 (MQ=255)
                                                 aGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTccc                                   >  2:2667976/1‑93 (MQ=255)
                                                  ggggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCct                                  <  1:4208840/93‑1 (MQ=255)
                                                    ggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc                                >  1:4347058/1‑93 (MQ=255)
                                                    ggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc                                >  1:1889211/1‑93 (MQ=255)
                                                    ggATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgc                                <  2:1876478/93‑1 (MQ=255)
                                                      aTCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgctg                              <  1:483255/93‑1 (MQ=255)
                                                      aTCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCctgctg                              <  1:1277259/93‑1 (MQ=255)
                                                       tCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtgg                             <  2:2361524/93‑1 (MQ=255)
                                                         gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           <  2:4456327/93‑1 (MQ=255)
                                                         gatgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtg                           <  2:3484262/93‑1 (MQ=255)
                                                          atgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCtggtgg                          >  2:1008885/1‑93 (MQ=255)
                                                           tgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTggg                         <  2:6266547/93‑1 (MQ=255)
                                                           tgcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTggg                         <  2:1661005/93‑1 (MQ=255)
                                                            gcgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGt                        <  2:4746117/93‑1 (MQ=255)
                                                             cgatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTa                       >  1:2840625/1‑93 (MQ=255)
                                                              gatgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAg                      >  1:4653860/1‑93 (MQ=255)
                                                               atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                     >  2:4158403/1‑93 (MQ=255)
                                                               atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                     >  1:6031081/1‑93 (MQ=255)
                                                               atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                     <  2:3879007/93‑1 (MQ=255)
                                                               atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                     <  2:3035033/93‑1 (MQ=255)
                                                               atgGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGc                     >  2:5864445/1‑93 (MQ=255)
                                                                  gTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGaa                  <  1:5415141/93‑1 (MQ=255)
                                                                    ttCCTCCTGGAACCGCGCAGCACCAAACGCCGGCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgc                <  1:3455085/93‑1 (MQ=255)
                                                                        tcctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgcgc            <  1:4049878/93‑1 (MQ=255)
                                                                        tccgGGAACCGCGCAGCGCCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAgcgcgc            <  2:4187927/93‑1 (MQ=255)
                                                                         cctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgcc           >  1:3247064/1‑93 (MQ=255)
                                                                         cctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgcc           <  2:3841945/93‑1 (MQ=255)
                                                                         cctGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGcgcc           >  2:74708/1‑93 (MQ=255)
                                                                            ggAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCgccgcc        >  2:5954406/1‑93 (MQ=255)
                                                                             gAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCa       >  1:1936830/1‑93 (MQ=255)
                                                                               aCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACg     >  2:4525125/1‑93 (MQ=255)
                                                                               aCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACg     >  2:631188/1‑93 (MQ=255)
                                                                                 cgcgCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTGCCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGt   <  2:1611948/93‑1 (MQ=255)
                                                                                 cgcgCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGt   <  2:1167964/93‑1 (MQ=255)
                                                                                       ||                                                                                      
ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT  >  NC_000913/3950470‑3950644

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  NC_000913/3950464‑3950646
                                                                                             |                                                                                         
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAG                                                                                           >  SRR4302534.1292327/1‑94 (MQ=60)
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAG                                                                                           >  SRR4302534.2356593/1‑94 (MQ=60)
     TATCGCTGTTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGTTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCA                                                                                      <  SRR4302534.5612338/94‑1 (MQ=60)
      ATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAA                                                                                     >  SRR4302534.4714081/1‑94 (MQ=60)
       TCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAA                                                                                    >  SRR4302534.3731537/1‑94 (MQ=60)
        CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   >  SRR4302534.953333/1‑94 (MQ=60)
        CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   >  SRR4302534.5860448/1‑94 (MQ=60)
        CGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAAC                                                                                   >  SRR4302534.5295575/1‑94 (MQ=60)
          CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                                 >  SRR4302534.3619621/1‑94 (MQ=60)
          CTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGCATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACAC                                                                                 >  SRR4302534.3382192/1‑94 (MQ=60)
              TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCTAACACCATC                                                                             <  SRR4302534.6471779/94‑1 (MQ=60)
              TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                             >  SRR4302534.1432696/1‑94 (MQ=60)
              TTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATC                                                                             <  SRR4302534.6478382/94‑1 (MQ=60)
               TGCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCC                                                                            <  SRR4302534.1771513/94‑1 (MQ=60)
                TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           >  SRR4302534.3135865/1‑94 (MQ=60)
                TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           >  SRR4302534.5566649/1‑94 (MQ=60)
                TCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCC                                                                           <  SRR4302534.1986895/94‑1 (MQ=60)
                 CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                          >  SRR4302534.5296525/1‑94 (MQ=60)
                 CCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCG                                                                          >  SRR4302534.1949164/1‑94 (MQ=60)
                    GTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                       >  SRR4302534.6062872/1‑94 (MQ=60)
                    GTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACG                                                                       <  SRR4302534.2023233/94‑1 (MQ=60)
                     TGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGG                                                                      <  SRR4302534.4321279/94‑1 (MQ=60)
                     TGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGG                                                                      >  SRR4302534.3688124/1‑94 (MQ=60)
                     TGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACAATCCCGACGG                                                                      >  SRR4302534.2190183/1‑94 (MQ=60)
                        GGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGG                                                                   >  SRR4302534.1029126/1‑94 (MQ=60)
                         GAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGC                                                                  <  SRR4302534.4311779/94‑1 (MQ=60)
                          AGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCA                                                                 >  SRR4302534.2115535/1‑94 (MQ=60)
                             GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                              >  SRR4302534.812960/1‑94 (MQ=60)
                             GTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAA                                                              >  SRR4302534.2964275/1‑94 (MQ=60)
                               ATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGC                                                            >  SRR4302534.1917525/1‑94 (MQ=60)
                               ATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGC                                                            >  SRR4302534.5641451/1‑94 (MQ=60)
                               ATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGC                                                            <  SRR4302534.5811377/94‑1 (MQ=60)
                               ATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGC                                                            <  SRR4302534.581946/94‑1 (MQ=60)
                                TCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCC                                                           <  SRR4302534.3235307/94‑1 (MQ=60)
                                 CTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCG                                                          >  SRR4302534.3017511/1‑94 (MQ=60)
                                  TGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGaagg                                                         >  SRR4302534.279299/1‑90 (MQ=60)
                                  TGCGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGG                                                         <  SRR4302534.1562634/94‑1 (MQ=60)
                                   GGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGG                                                        >  SRR4302534.3619326/1‑94 (MQ=60)
                                   GGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTGCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGT                                                        <  SRR4302534.2163645/94‑1 (MQ=60)
                                    GGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTG                                                       >  SRR4302534.4251968/1‑94 (MQ=60)
                                    GGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTG                                                       <  SRR4302534.632169/94‑1 (MQ=60)
                                       GCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTA                                                    <  SRR4302534.4743862/94‑1 (MQ=60)
                                       GCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTA                                                    >  SRR4302534.2495034/1‑94 (MQ=60)
                                       GCAGAAGCGCGGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTA                                                    <  SRR4302534.556221/94‑1 (MQ=60)
                                        CAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAA                                                   <  SRR4302534.5108863/94‑1 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGgcac                                                  >  SRR4302534.771878/1‑90 (MQ=60)
                                         AGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAAC                                                  >  SRR4302534.2758289/1‑94 (MQ=60)
                                          GAAGCGCTGGAGCAGGGGGTCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGACGGTGGTGACT                                                 >  SRR4302534.2503497/1‑94 (MQ=60)
                                          GAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACT                                                 <  SRR4302534.3103257/94‑1 (MQ=60)
                                          GAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACT                                                 <  SRR4302534.5114318/94‑1 (MQ=60)
                                           AAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTA                                                <  SRR4302534.576209/94‑1 (MQ=60)
                                           AAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTA                                                >  SRR4302534.6289021/1‑94 (MQ=60)
                                           AAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTA                                                <  SRR4302534.3931652/94‑1 (MQ=60)
                                               GCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTC                                            <  SRR4302534.1225139/94‑1 (MQ=60)
                                                  GGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                         >  SRR4302534.1376339/1‑94 (MQ=60)
                                                  GGAGCAGGGGATCGATGCGATGGTGTCCTCCTGGAACCGCGCAGCACCAAACACCACCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCT                                         <  SRR4302534.6457636/94‑1 (MQ=60)
                                                   GAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTT                                        >  SRR4302534.4118488/1‑94 (MQ=60)
                                                   GAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTT                                        >  SRR4302534.1808252/1‑94 (MQ=60)
                                                    AGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTC                                       <  SRR4302534.5094650/94‑1 (MQ=60)
                                                    AGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTC                                       >  SRR4302534.2793696/1‑94 (MQ=60)
                                                    AGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTC                                       <  SRR4302534.2720553/94‑1 (MQ=60)
                                                     GCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCC                                      >  SRR4302534.1660763/1‑94 (MQ=60)
                                                     GCAGGGGATCGATGCGATGGTTTCCTCCTCGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCC                                      >  SRR4302534.6008807/1‑94 (MQ=60)
                                                      CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                     >  SRR4302534.2182878/1‑94 (MQ=60)
                                                      CAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCC                                     >  SRR4302534.844626/1‑94 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    <  SRR4302534.4308487/94‑1 (MQ=60)
                                                       AGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCT                                    <  SRR4302534.2542620/94‑1 (MQ=60)
                                                        GGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTG                                   <  SRR4302534.4322415/94‑1 (MQ=60)
                                                         GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                  >  SRR4302534.1938477/1‑94 (MQ=60)
                                                         GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                  >  SRR4302534.198729/1‑94 (MQ=60)
                                                         GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                  <  SRR4302534.4265670/94‑1 (MQ=60)
                                                         GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                  >  SRR4302534.4464438/1‑94 (MQ=60)
                                                         GGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGC                                  >  SRR4302534.2833211/1‑94 (MQ=60)
                                                           GATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTG                                <  SRR4302534.3863653/94‑1 (MQ=60)
                                                           GATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTG                                >  SRR4302534.4184415/1‑94 (MQ=60)
                                                            ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                               <  SRR4302534.495364/94‑1 (MQ=60)
                                                            ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                               <  SRR4302534.1310874/94‑1 (MQ=60)
                                                            ATCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGG                               <  SRR4302534.3346757/94‑1 (MQ=60)
                                                             TCGATGCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGT                              <  SRR4302534.5589273/94‑1 (MQ=60)
                                                                  GCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTA                         <  SRR4302534.2731645/94‑1 (MQ=60)
                                                                  GCGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTA                         >  SRR4302534.2915255/1‑94 (MQ=60)
                                                                   CGATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAG                        >  SRR4302534.4780198/1‑94 (MQ=60)
                                                                    GATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGC                       >  SRR4302534.6194968/1‑94 (MQ=60)
                                                                     ATGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCG                      >  SRR4302534.3504362/1‑94 (MQ=60)
                                                                      TGGTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGA                     >  SRR4302534.5081871/1‑94 (MQ=60)
                                                                        GTTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAG                   <  SRR4302534.5560804/94‑1 (MQ=60)
                                                                         TTTCCTCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGC                  <  SRR4302534.4419035/94‑1 (MQ=60)
                                                                          TTCCTCCTGGAACCGCGCAGCACCAAACGCCGGCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCG                 <  SRR4302534.3547959/94‑1 (MQ=60)
                                                                           TCCTCCTGGACCCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGC                <  SRR4302534.1277220/94‑1 (MQ=60)
                                                                            CCTCCTGGAACCGCGAAGCACCAAAAACCATCCCGACGGCGGCAAAAGCCGGAGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCG               >  SRR4302534.1579078/1‑94 (MQ=60)
                                                                              TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC             >  SRR4302534.3333529/1‑94 (MQ=60)
                                                                              TCCTGGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC             <  SRR4302534.4158490/94‑1 (MQ=60)
                                                                              TCCTGGAACCGCGCAGAACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCC             >  SRR4302534.3341379/1‑94 (MQ=60)
                                                                                  GGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCA         >  SRR4302534.2966463/1‑94 (MQ=60)
                                                                                  GGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCA         >  SRR4302534.1456363/1‑94 (MQ=60)
                                                                                  GGAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCA         >  SRR4302534.1987468/1‑94 (MQ=60)
                                                                                   GAACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCAC        >  SRR4302534.3812337/1‑94 (MQ=60)
                                                                                    AACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGCGTAGCGAAGCGCGCCGCCACG       >  SRR4302534.2113762/1‑94 (MQ=60)
                                                                                     ACCGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGG      <  SRR4302534.4871453/94‑1 (MQ=60)
                                                                                       CGCGCAGCACCAAACACCATCCGGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT    <  SRR4302534.1523708/94‑1 (MQ=60)
                                                                                       CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT    <  SRR4302534.1765059/94‑1 (MQ=60)
                                                                                       CGCGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTT    <  SRR4302534.2451674/94‑1 (MQ=60)
                                                                                        GCGCAGCACCAACCACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTCCCTCTCTTCCCTGCTGGTGTGTAGCGAAGCGCGCCGCCACGGTTA   <  SRR4302534.4396252/94‑1 (MQ=60)
                                                                                        GCGCAGCACCAAACCCCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTA   <  SRR4302534.1176801/94‑1 (MQ=60)
                                                                                         CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  SRR4302534.1862787/1‑94 (MQ=60)
                                                                                         CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  <  SRR4302534.5675142/94‑1 (MQ=60)
                                                                                         CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  <  SRR4302534.4416278/94‑1 (MQ=60)
                                                                                         CGCAGCACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGAAGCGAAGCGCGCCGCCACGGTTAT  >  SRR4302534.5760785/1‑94 (MQ=60)
                                                                                             |                                                                                         
GTGATTATCGCTGCTTTCCCGTGGGGAGCGTATCTGGGCGCAGAAGCGCTGGAGCAGGGGATCGATGCGATGGTTTCCTCCTGGAACCGCGCACGACCAAACACCATCCCGACGGCGGCAAAAGCCGGTGGTAACTACCTCTCTTCCCTGCTGGTGGGTAGCGAAGCGCGCCGCCACGGTTAT  >  NC_000913/3950464‑3950646

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑