Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,969,103 2 bp→GC coding (232‑233/1263 nt) wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,1030CG99.8% 1910.3 / NA 560R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  major base G (250/309);  minor base T (1/0);  total (251/309)
Fisher's exact test for biased strand distribution p-value = 4.48e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009133,969,1040GC99.8% 1850.9 / NA 543R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base C (236/306);  minor base A (1/0);  total (237/306)
Fisher's exact test for biased strand distribution p-value = 4.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 578 total aligned reads displayed.

TCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC  >  NC_000913/3969011‑3969188
                                                                                            ||                                                                                    
     atCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctgg                                                                                  >  1:414363/1‑93 (MQ=255)
      tcgtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggc                                                                                 >  1:2503510/1‑93 (MQ=255)
       cgtcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct                                                                                <  2:3067536/93‑1 (MQ=255)
       cgtcgAACCTGATGTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGcctggct                                                                                <  1:2050490/93‑1 (MQ=255)
         tcgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa                                                                              <  1:8761162/93‑1 (MQ=255)
         tagAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGa                                                                              <  1:3740448/91‑1 (MQ=255)
          cgAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCAAGTTGAAGCGGATGCCTGGCTGAt                                                                             <  1:973117/93‑1 (MQ=255)
           gAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                            >  2:7997420/1‑93 (MQ=255)
           gAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                            >  2:7587122/1‑93 (MQ=255)
           gAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                            <  2:7056995/93‑1 (MQ=255)
            aaCCTGATTTGGCTAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTg                                                                           >  1:2123156/1‑93 (MQ=255)
             aCCTGATTTGGCGATTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                          <  2:7302592/93‑1 (MQ=255)
               cTGATTTGGCGAGTGTAGTAAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTg                                                                        <  2:466330/93‑1 (MQ=255)
                 gATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTa                                                                      >  2:6805288/1‑93 (MQ=255)
                   tttGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAcc                                                                    <  2:8745731/93‑1 (MQ=255)
                     tGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCa                                                                  <  2:2737655/93‑1 (MQ=255)
                      ggCGAGTGTAGTAAAAACTGCCGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc                                                                 <  1:2240739/93‑1 (MQ=255)
                       gCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAcc                                                                >  1:7244799/1‑92 (MQ=255)
                       gCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACg                                                                >  1:6154592/1‑93 (MQ=255)
                        cGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGc                                                               >  2:7473068/1‑93 (MQ=255)
                           gtgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt                                                            >  2:8499737/1‑93 (MQ=255)
                             gtagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt                                                          >  1:3353511/1‑93 (MQ=255)
                             gtagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt                                                          <  2:3864023/93‑1 (MQ=255)
                             gtagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt                                                          >  2:2089261/1‑93 (MQ=255)
                              tagtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTa                                                         >  1:2713097/1‑93 (MQ=255)
                               agtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa                                                        >  2:522488/1‑93 (MQ=255)
                                gtaAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                       >  1:8453028/1‑93 (MQ=255)
                                 taAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGGTGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg                                                      >  2:1783022/1‑93 (MQ=255)
                                 taAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg                                                      <  1:8363942/93‑1 (MQ=255)
                                 taAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgg                                                      <  1:7002946/93‑1 (MQ=255)
                                 taAAAACCGCCGTAGAAGGCGCTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGTCTGATTGCTGTACCCACGCCGTTTAAgg                                                      <  1:1083365/93‑1 (MQ=255)
                                  aaaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg                                                     <  1:4963246/93‑1 (MQ=255)
                                  aaaaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg                                                     <  1:472905/93‑1 (MQ=255)
                                    aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGGTGTACCCACGCCGTTTAAGGGCg                                                   >  2:4804078/1‑93 (MQ=255)
                                    aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg                                                   >  2:91592/1‑93 (MQ=255)
                                    aaaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg                                                   >  1:4062986/1‑93 (MQ=255)
                                     aaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAgggagt                                                  >  1:1322644/1‑90 (MQ=255)
                                     aaCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGa                                                  <  2:5057771/93‑1 (MQ=255)
                                       ctcccGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATc                                                <  1:7873444/90‑1 (MQ=255)
                                        tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa                                               <  1:1507597/93‑1 (MQ=255)
                                        tGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCa                                               <  2:2172201/93‑1 (MQ=255)
                                         gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAt                                              <  1:6398365/93‑1 (MQ=255)
                                         gCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAt                                              <  1:7460094/93‑1 (MQ=255)
                                           cGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa                                            <  1:387773/93‑1 (MQ=255)
                                            gTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCCCGCCGTTTAAGGGCGGTCATGac                                           >  2:2869815/1‑92 (MQ=255)
                                            gTAAAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg                                           <  1:1867149/93‑1 (MQ=255)
                                             tAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGc                                          >  2:371955/1‑93 (MQ=255)
                                               gAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa                                        >  2:4563168/1‑93 (MQ=255)
                                                aaGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAg                                       >  1:1018684/1‑93 (MQ=255)
                                                 aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGa                                      >  2:1091520/1‑93 (MQ=255)
                                                 aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGa                                      <  1:4244056/93‑1 (MQ=255)
                                                 aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGa                                      >  2:660915/1‑93 (MQ=255)
                                                 aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGa                                      <  2:819925/93‑1 (MQ=255)
                                                 aGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGa                                      >  2:7716073/1‑93 (MQ=255)
                                                  ggCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGCTCATGAGCCAGat                                     >  2:4367093/1‑93 (MQ=255)
                                                  ggCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat                                     >  1:6580603/1‑93 (MQ=255)
                                                  ggCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat                                     >  1:5245923/1‑93 (MQ=255)
                                                  ggCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat                                     >  2:1263324/1‑93 (MQ=255)
                                                   gCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                    >  1:6744072/1‑93 (MQ=255)
                                                       ttttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc                                <  1:7768302/93‑1 (MQ=255)
                                                       ttttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc                                <  2:1611269/93‑1 (MQ=255)
                                                       ttttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAc                                >  2:834623/1‑93 (MQ=255)
                                                        tttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc                               <  2:2551505/93‑1 (MQ=255)
                                                        tttttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc                               <  1:8581540/93‑1 (MQ=255)
                                                          tttACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTa                             <  2:2396305/93‑1 (MQ=255)
                                                            tACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACg                           <  1:3641977/93‑1 (MQ=255)
                                                             aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGt                          <  2:6250858/93‑1 (MQ=255)
                                                             aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGt                          >  2:5495811/1‑93 (MQ=255)
                                                               gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg                        <  1:5834578/93‑1 (MQ=255)
                                                               gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg                        >  1:6257647/1‑93 (MQ=255)
                                                                agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGa                       >  2:585919/1‑93 (MQ=255)
                                                                  cgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt                     <  1:5460502/93‑1 (MQ=255)
                                                                    agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCg                   <  2:4207824/93‑1 (MQ=255)
                                                                    agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCg                   >  1:310954/1‑93 (MQ=255)
                                                                    agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCg                   >  2:5375343/1‑93 (MQ=255)
                                                                     gcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCgg                  >  2:1919158/1‑93 (MQ=255)
                                                                      cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                 <  1:7992860/93‑1 (MQ=255)
                                                                      cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                 <  1:5677712/93‑1 (MQ=255)
                                                                      cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                 <  2:5989112/93‑1 (MQ=255)
                                                                       acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                <  2:3579680/93‑1 (MQ=255)
                                                                       acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                <  1:8255487/93‑1 (MQ=255)
                                                                       acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                <  2:341233/93‑1 (MQ=255)
                                                                        cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctg               >  1:4506672/1‑93 (MQ=255)
                                                                          acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct             >  1:5612837/1‑93 (MQ=255)
                                                                          acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct             >  1:3241805/1‑93 (MQ=255)
                                                                          acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct             >  2:3929445/1‑93 (MQ=255)
                                                                          acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct             <  1:482688/93‑1 (MQ=255)
                                                                          acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct             <  2:410444/93‑1 (MQ=255)
                                                                          acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct             <  2:3215310/93‑1 (MQ=255)
                                                                            gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcg           >  2:1440559/1‑93 (MQ=255)
                                                                            gCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcg           >  2:5756433/1‑93 (MQ=255)
                                                                             ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgc          <  1:1367715/93‑1 (MQ=255)
                                                                               aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgctc        <  2:2251125/93‑1 (MQ=255)
                                                                                gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTcc       <  1:2356748/93‑1 (MQ=255)
                                                                                gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTcc       >  1:605725/1‑93 (MQ=255)
                                                                                 ttGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGACTCGGCTGCTCGCTCCa      >  1:1697210/1‑93 (MQ=255)
                                                                                   gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAtt    <  1:7373901/93‑1 (MQ=255)
                                                                                   gAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGACAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAtt    >  1:4342295/1‑93 (MQ=255)
                                                                                    aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTg   <  1:2828405/93‑1 (MQ=255)
                                                                                     aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgc  <  1:7565354/93‑1 (MQ=255)
                                                                                            ||                                                                                    
TCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC  >  NC_000913/3969011‑3969188

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 321 total aligned reads displayed.
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  NC_000913/3969010‑3969192
                                                                                              |                                                                                         
ATCCATATCGTCGAACCTGATTTGGCGATTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATG                                                                                           <  SRR4302541.764899/94‑1 (MQ=60)
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATG                                                                                           <  SRR4302541.8449508/94‑1 (MQ=60)
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATG                                                                                           <  SRR4302541.2615587/94‑1 (MQ=60)
     TATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGG                                                                                      <  SRR4302541.5533797/94‑1 (MQ=60)
     TATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGG                                                                                      >  SRR4302541.418207/1‑94 (MQ=60)
      ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGC                                                                                     >  SRR4302541.2528763/1‑94 (MQ=60)
      ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGC                                                                                     >  SRR4302541.126988/1‑94 (MQ=60)
      ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGC                                                                                     <  SRR4302541.6164624/94‑1 (MQ=60)
      ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGC                                                                                     >  SRR4302541.3941487/1‑94 (MQ=60)
      ATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGC                                                                                     >  SRR4302541.4779252/1‑94 (MQ=60)
        CGTCGAACCTGATGTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTG                                                                                   <  SRR4302541.2070280/94‑1 (MQ=60)
          tctaaacctgtgTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                 <  SRR4302541.2730907/82‑1 (MQ=60)
          TCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                 >  SRR4302541.5422414/1‑94 (MQ=60)
          TCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                 <  SRR4302541.2745121/94‑1 (MQ=60)
          TAGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                 <  SRR4302541.3778090/94‑1 (MQ=60)
           CGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGTTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATT                                                                                <  SRR4302541.6494185/94‑1 (MQ=60)
           CGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATT                                                                                <  SRR4302541.6675212/94‑1 (MQ=60)
           CGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATT                                                                                <  SRR4302541.6967854/94‑1 (MQ=60)
           CGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCAAGTTGAAGCGGATGCCTGGCTGATT                                                                                <  SRR4302541.982250/94‑1 (MQ=60)
            GACCCTTATTTGGCGAGTGTAGAAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               <  SRR4302541.3596160/94‑1 (MQ=60)
            GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               >  SRR4302541.1040650/1‑94 (MQ=60)
            GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               <  SRR4302541.7411883/94‑1 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                              <  SRR4302541.4174218/94‑1 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                              >  SRR4302541.8540311/1‑94 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                              >  SRR4302541.6130165/1‑94 (MQ=60)
                CTTATTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGTTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                           <  SRR4302541.6459236/94‑1 (MQ=60)
                 TGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTA                                                                          >  SRR4302541.4720280/1‑94 (MQ=60)
                 TGATTGGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTA                                                                          <  SRR4302541.8323975/94‑1 (MQ=60)
                  GATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAC                                                                         >  SRR4302541.6146750/1‑94 (MQ=60)
                   ATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                        <  SRR4302541.7456156/94‑1 (MQ=60)
                   ATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                        <  SRR4302541.4412135/94‑1 (MQ=60)
                   ATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                        >  SRR4302541.2418821/1‑94 (MQ=60)
                   ATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATGTCTGTACC                                                                        >  SRR4302541.2418552/1‑94 (MQ=60)
                     TTGGCGAGTGTTGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCA                                                                      <  SRR4302541.6873664/94‑1 (MQ=60)
                      TGGCGAGTGTAGAAAAAACTGCCGTAGAAGGCGTTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                     <  SRR4302541.7858186/94‑1 (MQ=60)
                       GGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                    >  SRR4302541.6217481/1‑94 (MQ=60)
                       GGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACC                                                                    >  SRR4302541.7322072/1‑94 (MQ=60)
                         cgggttatgtaaaaacttccgtagaagttctTTTTTTAGGA‑GCGGGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  <  SRR4302541.1781350/63‑1 (MQ=58)
                         GGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  <  SRR4302541.590209/94‑1 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  >  SRR4302541.2310808/1‑94 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  >  SRR4302541.1723757/1‑94 (MQ=60)
                           AGTGTAGTAAAAACTGCCGTAGAAGGCTGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                <  SRR4302541.3496780/94‑1 (MQ=60)
                           AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                <  SRR4302541.746579/94‑1 (MQ=60)
                           AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                <  SRR4302541.7228527/94‑1 (MQ=60)
                           AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                >  SRR4302541.6331022/1‑94 (MQ=60)
                           AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTGACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                <  SRR4302541.3655804/94‑1 (MQ=60)
                            GTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                               >  SRR4302541.214859/1‑94 (MQ=60)
                            GTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                               <  SRR4302541.750848/94‑1 (MQ=60)
                            GTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                               >  SRR4302541.3700889/1‑94 (MQ=60)
                             gtgagaAAAAACTACCGGAGAAGGAGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              <  SRR4302541.7122260/88‑1 (MQ=60)
                             TGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              >  SRR4302541.3386992/1‑94 (MQ=60)
                             TGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              <  SRR4302541.1093261/94‑1 (MQ=60)
                             TGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              >  SRR4302541.1272727/1‑94 (MQ=60)
                             TGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              <  SRR4302541.5233868/94‑1 (MQ=60)
                              GTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTA                                                             >  SRR4302541.6718026/1‑94 (MQ=60)
                              GTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTA                                                             >  SRR4302541.2740134/1‑94 (MQ=60)
                                AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                           >  SRR4302541.6386568/1‑94 (MQ=60)
                                AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                           <  SRR4302541.8224375/94‑1 (MQ=60)
                                 GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCCCGCCGTTTAAGG                                                          >  SRR4302541.7378784/1‑94 (MQ=60)
                                 GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          <  SRR4302541.4232277/94‑1 (MQ=60)
                                 GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          <  SRR4302541.4355012/94‑1 (MQ=60)
                                 ATAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          <  SRR4302541.7674086/94‑1 (MQ=60)
                                  aaaaaccggcgGTAGAAGGCGTTTTTTTACAA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                         <  SRR4302541.3367692/83‑1 (MQ=60)
                                  TAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGCGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                         >  SRR4302541.7230913/1‑94 (MQ=60)
                                  TAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                         <  SRR4302541.3010691/94‑1 (MQ=60)
                                  TAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                         <  SRR4302541.8458697/94‑1 (MQ=60)
                                  TAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                         <  SRR4302541.8454643/94‑1 (MQ=60)
                                  AAAAAACTGCCGTAGAAGGCGTTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                         <  SRR4302541.3312039/94‑1 (MQ=60)
                                   AAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGG                                                        >  SRR4302541.6580082/1‑94 (MQ=60)
                                   AAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        <  SRR4302541.5014064/94‑1 (MQ=60)
                                   AAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        <  SRR4302541.477398/94‑1 (MQ=60)
                                   AAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        <  SRR4302541.4559724/94‑1 (MQ=60)
                                   AAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        >  SRR4302541.8399119/1‑94 (MQ=60)
                                   AAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        <  SRR4302541.8375729/94‑1 (MQ=60)
                                    AAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       >  SRR4302541.4103085/1‑94 (MQ=60)
                                    AAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       <  SRR4302541.5701850/94‑1 (MQ=60)
                                     aaccgcctgaagagggtttTTTTTTGCGA‑GCGACCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                      <  SRR4302541.7775547/75‑1 (MQ=60)
                                     AAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                      >  SRR4302541.1335653/1‑94 (MQ=60)
                                     AAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                      <  SRR4302541.8570927/94‑1 (MQ=60)
                                     AAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                      <  SRR4302541.8523687/94‑1 (MQ=60)
                                       ACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATC                                                    <  SRR4302541.868799/94‑1 (MQ=60)
                                        CTCCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCA                                                   <  SRR4302541.7959955/94‑1 (MQ=60)
                                         TGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAT                                                  <  SRR4302541.1522564/94‑1 (MQ=60)
                                         TGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAT                                                  >  SRR4302541.2154533/1‑94 (MQ=60)
                                         TGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCAT                                                  <  SRR4302541.8547556/94‑1 (MQ=60)
                                          GCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATG                                                 <  SRR4302541.7539790/94‑1 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               >  SRR4302541.3074431/1‑94 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               >  SRR4302541.4167376/1‑94 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               <  SRR4302541.4052652/94‑1 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               <  SRR4302541.391357/94‑1 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               <  SRR4302541.5815274/94‑1 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTACTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               >  SRR4302541.1408929/1‑94 (MQ=60)
                                             GTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGC                                              >  SRR4302541.5142233/1‑94 (MQ=60)
                                             GTAGAAGG‑GGTTTTTTAAGAGGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGC                                              <  SRR4302541.4891425/94‑1 (MQ=60)
                                             GTAAAAGGCGTTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGC                                              <  SRR4302541.1885136/94‑1 (MQ=60)
                                              TAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                             >  SRR4302541.4471247/1‑94 (MQ=60)
                                              TAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                             >  SRR4302541.1398624/1‑94 (MQ=60)
                                              TAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                             <  SRR4302541.1536934/94‑1 (MQ=60)
                                              TAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                             >  SRR4302541.4850729/1‑94 (MQ=60)
                                               AGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            <  SRR4302541.5627781/94‑1 (MQ=60)
                                               AGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            <  SRR4302541.1376204/94‑1 (MQ=60)
                                               AGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            <  SRR4302541.2827352/94‑1 (MQ=60)
                                                GAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAG                                           >  SRR4302541.1028098/1‑94 (MQ=60)
                                                GAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGACAG                                           >  SRR4302541.4131041/1‑94 (MQ=60)
                                                 AAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAATGGCGATCATGAGCCAGA                                          >  SRR4302541.7861919/1‑94 (MQ=60)
                                                 AAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGA                                          >  SRR4302541.1929129/1‑94 (MQ=60)
                                                  AGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                         >  SRR4302541.5029089/1‑94 (MQ=60)
                                                  AGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                         <  SRR4302541.7546206/94‑1 (MQ=60)
                                                  AGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGAT                                         >  SRR4302541.7604126/1‑94 (MQ=60)
                                                   GGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATA                                        >  SRR4302541.4839029/1‑94 (MQ=60)
                                                   GGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATA                                        >  SRR4302541.6816126/1‑94 (MQ=60)
                                                    GCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       <  SRR4302541.6128901/94‑1 (MQ=60)
                                                    GCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       <  SRR4302541.3788093/94‑1 (MQ=60)
                                                     ggggTTTTTACGA‑GCGAGCACGACGTCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATG                                      <  SRR4302541.5572590/90‑1 (MQ=60)
                                                      GGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                     >  SRR4302541.5368248/1‑94 (MQ=60)
                                                      GGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                     >  SRR4302541.2090017/1‑94 (MQ=60)
                                                      GGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                     <  SRR4302541.8221897/94‑1 (MQ=60)
                                                        TTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACC                                   <  SRR4302541.7850936/94‑1 (MQ=60)
                                                         TTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                                  <  SRR4302541.4815609/94‑1 (MQ=60)
                                                         TTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                                  <  SRR4302541.2136101/94‑1 (MQ=60)
                                                         TTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                                  <  SRR4302541.8673669/94‑1 (MQ=60)
                                                          TTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTA                                 <  SRR4302541.1441835/94‑1 (MQ=60)
                                                           TTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302541.206187/94‑1 (MQ=60)
                                                           TTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302541.1374460/94‑1 (MQ=60)
                                                           TTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302541.904281/94‑1 (MQ=60)
                                                           TTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302541.4332609/94‑1 (MQ=60)
                                                            TTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACG                               >  SRR4302541.2281873/1‑94 (MQ=60)
                                                             TACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGT                              >  SRR4302541.4377735/1‑94 (MQ=60)
                                                             TACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGT                              >  SRR4302541.1666413/1‑94 (MQ=60)
                                                              ACGC‑GCGAGCACGACGGCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACACACGCCGTTTACGGGCGATCATGAGCCAGATATGACCTACGTT                             <  SRR4302541.3257379/94‑1 (MQ=60)
                                                              ACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGTGATCATGAGCCAGATATGACCTACGTT                             <  SRR4302541.2191903/94‑1 (MQ=60)
                                                              ACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGTCAGATATGACCTACGTT                             >  SRR4302541.7931535/1‑94 (MQ=60)
                                                              ACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTT                             <  SRR4302541.3257378/94‑1 (MQ=60)
                                                              ACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTT                             <  SRR4302541.4908100/94‑1 (MQ=60)
                                                              ACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTT                             <  SRR4302541.3694770/94‑1 (MQ=60)
                                                              ACGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTT                             >  SRR4302541.6017696/1‑94 (MQ=60)
                                                               CGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTG                            >  SRR4302541.2102703/1‑94 (MQ=60)
                                                               CGA‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTG                            <  SRR4302541.3903994/94‑1 (MQ=60)
                                                                 A‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAA                          >  SRR4302541.1770636/1‑94 (MQ=60)
                                                                 A‑GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAA                          <  SRR4302541.2801689/94‑1 (MQ=60)
                                                                   GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAT                         <  SRR4302541.6653944/94‑1 (MQ=60)
                                                                   GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAT                         <  SRR4302541.2186347/94‑1 (MQ=60)
                                                                    CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        <  SRR4302541.8496861/94‑1 (MQ=60)
                                                                    CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        <  SRR4302541.3760445/94‑1 (MQ=60)
                                                                    CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        <  SRR4302541.5516514/94‑1 (MQ=60)
                                                                    CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        <  SRR4302541.6334297/94‑1 (MQ=60)
                                                                    CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCATTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        <  SRR4302541.773982/94‑1 (MQ=60)
                                                                     GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       >  SRR4302541.4748499/1‑94 (MQ=60)
                                                                     GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       <  SRR4302541.574154/94‑1 (MQ=60)
                                                                     GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       >  SRR4302541.1997404/1‑94 (MQ=60)
                                                                     GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       <  SRR4302541.2921296/94‑1 (MQ=60)
                                                                     GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       <  SRR4302541.4389470/94‑1 (MQ=60)
                                                                     GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       >  SRR4302541.313714/1‑94 (MQ=60)
                                                                     GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       >  SRR4302541.6272244/1‑94 (MQ=60)
                                                                     GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       >  SRR4302541.3184162/1‑94 (MQ=60)
                                                                      AGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGG                      >  SRR4302541.3315230/1‑94 (MQ=60)
                                                                      AGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGG                      <  SRR4302541.5677957/94‑1 (MQ=60)
                                                                       GCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCCCGCCGTTTAAGGGCGCTCATGAGCCAGATATGACCTACTTTGAATCGGC                     >  SRR4302541.1344077/1‑94 (MQ=60)
                                                                       GCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGC                     <  SRR4302541.8601996/94‑1 (MQ=60)
                                                                        CACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                    <  SRR4302541.5735644/94‑1 (MQ=60)
                                                                        CACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                    <  SRR4302541.8080852/94‑1 (MQ=60)
                                                                        CACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCT                    <  SRR4302541.8775735/94‑1 (MQ=60)
                                                                         ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   <  SRR4302541.8345383/94‑1 (MQ=60)
                                                                         ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   <  SRR4302541.6288774/94‑1 (MQ=60)
                                                                         ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   <  SRR4302541.7037102/94‑1 (MQ=60)
                                                                         ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   >  SRR4302541.2710019/1‑94 (MQ=60)
                                                                          CGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGC                  >  SRR4302541.7986670/1‑94 (MQ=60)
                                                                           GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCGCGCCGTTTAAGCGCGATCATGAGCCAGATATGACCTAAGTTGAATCGGCTGCT                 <  SRR4302541.7600742/94‑1 (MQ=60)
                                                                           GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 >  SRR4302541.1058789/1‑94 (MQ=60)
                                                                           GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 <  SRR4302541.2697836/94‑1 (MQ=60)
                                                                           GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 <  SRR4302541.4045500/94‑1 (MQ=60)
                                                                           GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 >  SRR4302541.3274528/1‑94 (MQ=60)
                                                                            ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                <  SRR4302541.1671482/94‑1 (MQ=60)
                                                                            ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                <  SRR4302541.5651765/94‑1 (MQ=60)
                                                                            ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                <  SRR4302541.4913472/94‑1 (MQ=60)
                                                                             CGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG               <  SRR4302541.4228565/94‑1 (MQ=60)
                                                                             CGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG               >  SRR4302541.804391/1‑94 (MQ=60)
                                                                             CGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG               <  SRR4302541.7093392/94‑1 (MQ=60)
                                                                             CGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG               <  SRR4302541.5789388/94‑1 (MQ=60)
                                                                              GCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGTGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGC              >  SRR4302541.935999/1‑94 (MQ=60)
                                                                              GCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGC              >  SRR4302541.4255653/1‑94 (MQ=60)
                                                                               CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCT             >  SRR4302541.5329023/1‑94 (MQ=60)
                                                                               CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCT             >  SRR4302541.6112153/1‑94 (MQ=60)
                                                                               CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCT             <  SRR4302541.1232550/94‑1 (MQ=60)
                                                                                 AGTTGAAGCGGATGCCTGGGTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCC           <  SRR4302541.6229417/94‑1 (MQ=60)
                                                                                 AGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCC           >  SRR4302541.5677613/1‑94 (MQ=60)
                                                                                  GTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCA          <  SRR4302541.2507309/94‑1 (MQ=60)
                                                                                  GTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCA          <  SRR4302541.6583355/94‑1 (MQ=60)
                                                                                    TGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT        <  SRR4302541.8913587/94‑1 (MQ=60)
                                                                                     GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       <  SRR4302541.2360939/94‑1 (MQ=60)
                                                                                     GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       >  SRR4302541.5987774/1‑94 (MQ=60)
                                                                                     GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       <  SRR4302541.6236690/94‑1 (MQ=60)
                                                                                     GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       <  SRR4302541.7452719/94‑1 (MQ=60)
                                                                                      AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC      <  SRR4302541.4489004/94‑1 (MQ=60)
                                                                                      AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC      <  SRR4302541.8810598/94‑1 (MQ=60)
                                                                                      AAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGC      <  SRR4302541.2856913/94‑1 (MQ=60)
                                                                                       AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     >  SRR4302541.1330260/1‑94 (MQ=60)
                                                                                          GGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  SRR4302541.6453969/1‑94 (MQ=60)
                                                                                          GGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  <  SRR4302541.7743867/94‑1 (MQ=60)
                                                                                          GGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  SRR4302541.6984032/1‑94 (MQ=60)
                                                                                              |                                                                                         
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGA‑GCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  NC_000913/3969010‑3969192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑