Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,969,103 2 bp→GC coding (232‑233/1263 nt) wecC → UDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,969,1030CG100.0% 774.3 / NA 222R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (111/111);  total (111/111)
*NC_0009133,969,1040GC100.0% 756.8 / NA 220R78A (CGC→GCC) wecCUDP‑N‑acetyl‑D‑mannosaminuronic acid dehydrogenase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (111/109);  total (111/109)

Only 100 of 222 total aligned reads displayed.

CGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG  >  NC_000913/3969018‑3969189
                                                                                      ||                                                                                     
    gacccTGTTGTGGCGAGTGTAGTAAAACCTGCCGTAGAAGGCGTTTTTTA‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAtt                                                                             <  1:5568434/90‑1 (MQ=255)
     aaCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTg                                                                            >  1:883951/1‑93 (MQ=255)
     aaCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTg                                                                            >  1:2680664/1‑93 (MQ=255)
      aCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                           <  1:1838256/93‑1 (MQ=255)
      aCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGc                                                                           <  2:4651353/93‑1 (MQ=255)
        cTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTg                                                                         >  2:1191408/1‑93 (MQ=255)
         tGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGt                                                                        >  2:1037921/1‑93 (MQ=255)
          gATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGtc                                                                       >  2:3611116/1‑92 (MQ=255)
           aTTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAc                                                                      >  1:5835845/1‑93 (MQ=255)
           aTTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAc                                                                      >  2:535803/1‑93 (MQ=255)
           aTTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAc                                                                      <  2:1496877/93‑1 (MQ=255)
               ggCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAc                                                                  >  1:1084604/1‑93 (MQ=255)
                 ggAGTGTAGAAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGc                                                                <  1:5666949/92‑1 (MQ=255)
                  gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc                                                               >  1:2764536/1‑93 (MQ=255)
                  gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc                                                               >  1:5806017/1‑93 (MQ=255)
                  gAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGcc                                                               >  2:6300455/1‑93 (MQ=255)
                    gtgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt                                                             <  1:4672671/93‑1 (MQ=255)
                    gtgtAGAAAAACCTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGt                                                             <  1:5770449/93‑1 (MQ=255)
                     tgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGtt                                                            <  2:2668222/93‑1 (MQ=255)
                     tgtAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGtt                                                            <  1:850600/93‑1 (MQ=255)
                      gtagtaAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt                                                           <  1:4033391/93‑1 (MQ=255)
                      gaggtaAAACGTCCCGAAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGttt                                                           <  1:2413777/90‑1 (MQ=255)
                       tagtaAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTa                                                          <  1:836036/93‑1 (MQ=255)
                        agtaAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa                                                         >  2:5243308/1‑93 (MQ=255)
                        agtaAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTaa                                                         >  2:5577558/1‑93 (MQ=255)
                         gtaAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                        <  1:2108730/93‑1 (MQ=255)
                         gtaAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAg                                                        <  1:6225689/93‑1 (MQ=255)
                           aaaaaCTGCCGTAGAAGGCGTTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg                                                      <  1:5750199/93‑1 (MQ=255)
                           aaaaaCTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAggg                                                      >  1:4088/1‑93 (MQ=255)
                            aaaaCTGGCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                     >  1:6359643/1‑93 (MQ=255)
                            aaaaCTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                     >  1:4762109/1‑93 (MQ=255)
                            aaaaCTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGc                                                     <  2:4674186/93‑1 (MQ=255)
                             aaaCTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg                                                    >  2:6005859/1‑93 (MQ=255)
                             aaaCTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCg                                                    <  1:652943/93‑1 (MQ=255)
                              aaCTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGTGCGa                                                   >  2:2709419/1‑93 (MQ=255)
                              aaCTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGa                                                   >  2:233535/1‑93 (MQ=255)
                              aaCTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGa                                                   >  2:3274222/1‑93 (MQ=255)
                              aaCTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGa                                                   >  1:1364146/1‑93 (MQ=255)
                              aaCTGCCGTAGAACGAGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGa                                                   <  2:4263808/93‑1 (MQ=255)
                               aCTGCCGTAGAAGGCGTTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAt                                                  <  2:5592432/93‑1 (MQ=255)
                                cTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCCGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAAGCCGGTTAAGGGCGATc                                                 >  1:3327983/1‑93 (MQ=255)
                                cTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATc                                                 <  2:3961449/93‑1 (MQ=255)
                                   cggTAGAGGGCGTTTTTTA‑ACGAGCGACCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATg                                              <  1:1144240/91‑1 (MQ=255)
                                   ccGTAGAAGGCTGTTTTTT‑ACAAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATg                                              <  1:3550426/93‑1 (MQ=255)
                                    cGTAGAAGGCGGTTTTTT‑ACCAGCGTGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGa                                             <  1:1393854/93‑1 (MQ=255)
                                     gTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg                                            >  2:6269020/1‑93 (MQ=255)
                                     gTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAg                                            <  1:1676721/93‑1 (MQ=255)
                                       aGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGcc                                          >  1:452968/1‑93 (MQ=255)
                                       aGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAGCATGAGcc                                          <  1:6279369/93‑1 (MQ=255)
                                        gAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa                                         >  1:1779194/1‑93 (MQ=255)
                                        gAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCa                                         <  1:6076028/93‑1 (MQ=255)
                                          aGGGGGTTTTTTGAGGAGCGGCCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGGGCCAg                                        <  1:39582/93‑1 (MQ=255)
                                           ggCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat                                      >  2:877081/1‑93 (MQ=255)
                                           ggCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGat                                      <  1:4350537/93‑1 (MQ=255)
                                            gCGTTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                     <  2:4508859/93‑1 (MQ=255)
                                            gCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGata                                     <  2:4294204/93‑1 (MQ=255)
                                             cGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGatat                                    <  1:4024376/93‑1 (MQ=255)
                                              ggTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATg                                   <  2:573511/93‑1 (MQ=255)
                                               gTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGa                                  >  1:2902175/1‑93 (MQ=255)
                                                 ttttt‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc                                <  2:3785560/93‑1 (MQ=255)
                                                 ttttt‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGAcc                                <  2:3551855/93‑1 (MQ=255)
                                                  tttt‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCt                               >  2:2294248/1‑93 (MQ=255)
                                                    tt‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAc                             <  1:3738643/93‑1 (MQ=255)
                                                       aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGt                           <  2:668408/93‑1 (MQ=255)
                                                       aCGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGt                           <  1:2930671/93‑1 (MQ=255)
                                                        cgagcgagCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGtt                          >  1:5105339/1‑93 (MQ=255)
                                                         gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg                         <  2:2985893/93‑1 (MQ=255)
                                                         gagcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTg                         >  1:5900243/1‑93 (MQ=255)
                                                          agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGa                        <  1:3487897/93‑1 (MQ=255)
                                                          agcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGa                        >  2:5149362/1‑93 (MQ=255)
                                                           gcgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGaa                       >  2:6415842/1‑93 (MQ=255)
                                                            cgagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAt                      >  1:324663/1‑93 (MQ=255)
                                                             gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                     >  1:3208657/1‑93 (MQ=255)
                                                             gagcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATc                     <  2:29357/93‑1 (MQ=255)
                                                              agcACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCg                    >  1:2325118/1‑93 (MQ=255)
                                                                cACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgc                  >  2:2624169/1‑93 (MQ=255)
                                                                 acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                 >  2:2829419/1‑93 (MQ=255)
                                                                 acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                 <  1:2022195/93‑1 (MQ=255)
                                                                 acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                 >  2:3448553/1‑93 (MQ=255)
                                                                 acgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGACCATGAGCCAGATATGACCTACGTTGAATCggtt                 >  2:4977618/1‑91 (MQ=255)
                                                                 acgacgCCAGTTGAAGCGGATGCCGGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgct                 <  2:1790338/93‑1 (MQ=255)
                                                                  cgacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctg                >  1:4108376/1‑93 (MQ=255)
                                                                   gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgc               >  1:1533734/1‑93 (MQ=255)
                                                                   gacgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgc               >  1:196737/1‑93 (MQ=255)
                                                                    acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct              >  1:2971256/1‑93 (MQ=255)
                                                                    acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct              >  2:1955441/1‑93 (MQ=255)
                                                                    acgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGgctgct              <  1:4920256/93‑1 (MQ=255)
                                                                     cgCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctc             >  1:5221442/1‑93 (MQ=255)
                                                                       ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgc           <  2:1684452/93‑1 (MQ=255)
                                                                       ccAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgc           <  2:6385541/93‑1 (MQ=255)
                                                                        cAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgatcgct          >  1:4990986/1‑93 (MQ=255)
                                                                         aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgctc         >  1:5926086/1‑93 (MQ=255)
                                                                         aGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTgctcgctc         >  2:5850853/1‑93 (MQ=255)
                                                                          gTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTcc        <  2:2270121/93‑1 (MQ=255)
                                                                            tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAt      <  1:4248224/93‑1 (MQ=255)
                                                                            tGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCAt      <  2:2069974/93‑1 (MQ=255)
                                                                              aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTg    >  1:1619746/1‑93 (MQ=255)
                                                                              aaGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTg    <  2:4121302/93‑1 (MQ=255)
                                                                               aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgc   <  1:3754901/93‑1 (MQ=255)
                                                                               aGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTgc   <  1:309143/93‑1 (MQ=255)
                                                                                      ||                                                                                     
CGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTT‑ACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG  >  NC_000913/3969018‑3969189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  NC_000913/3969010‑3969192
                                                                                             |                                                                                         
ATCCATATCGTCGATCCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATG                                                                                           <  SRR4302534.4014394/94‑1 (MQ=60)
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATG                                                                                           >  SRR4302534.951340/1‑94 (MQ=60)
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATG                                                                                           <  SRR4302534.2084245/94‑1 (MQ=60)
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATG                                                                                           >  SRR4302534.3104491/1‑94 (MQ=60)
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATG                                                                                           <  SRR4302534.4451994/94‑1 (MQ=60)
       TCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCT                                                                                    <  SRR4302534.1098739/94‑1 (MQ=60)
       TCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCT                                                                                    <  SRR4302534.721913/94‑1 (MQ=60)
         GTCGAACCTGATTGGGCGAGTGTAGAAAAAACTGCCGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGA                                                                                  <  SRR4302534.3589880/94‑1 (MQ=60)
          TCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGAT                                                                                 >  SRR4302534.4965033/1‑94 (MQ=60)
            gaccctgttgTGGCGAGTGTAGTAAAACCTGCCGTAGAAGGCGTTTTTTAACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               <  SRR4302534.5718809/84‑1 (MQ=60)
            GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               >  SRR4302534.2751520/1‑94 (MQ=60)
            GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               >  SRR4302534.3651359/1‑94 (MQ=60)
            GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               >  SRR4302534.705121/1‑94 (MQ=60)
            GAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTG                                                                               >  SRR4302534.906316/1‑94 (MQ=60)
             AACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGC                                                                              >  SRR4302534.4118465/1‑94 (MQ=60)
              ACTTGATTTGGCGTGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             <  SRR4302534.3482327/94‑1 (MQ=60)
              ACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             <  SRR4302534.1886049/94‑1 (MQ=60)
              ACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             >  SRR4302534.296122/1‑94 (MQ=60)
              ACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCT                                                                             >  SRR4302534.2811473/1‑94 (MQ=60)
               CCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTG                                                                            >  SRR4302534.5992843/1‑94 (MQ=60)
                CTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGGTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGT                                                                           >  SRR4302534.1772465/1‑94 (MQ=60)
                 tttttgggcgagtgaaaaaaaacCTGCCGTAGAAGGCGTTTTTTTACGAGCGACCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTA                                                                          <  SRR4302534.5704164/71‑1 (MQ=60)
                 TGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTA                                                                          >  SRR4302534.3887199/1‑94 (MQ=60)
                  GATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTAC                                                                         >  SRR4302534.5994278/1‑94 (MQ=60)
                   ATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACC                                                                        >  SRR4302534.356782/1‑94 (MQ=60)
                      TGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                     <  SRR4302534.6197153/94‑1 (MQ=60)
                      TGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAC                                                                     >  SRR4302534.1112805/1‑94 (MQ=60)
                       GGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                    <  SRR4302534.4084916/94‑1 (MQ=60)
                       GGCGAGTGTAGAAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACG                                                                    <  SRR4302534.1285033/94‑1 (MQ=60)
                        GCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGC                                                                   >  SRR4302534.2426995/1‑94 (MQ=60)
                         GGAGTGTAGAAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  <  SRR4302534.5820366/94‑1 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  >  SRR4302534.4418700/1‑94 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  >  SRR4302534.2837322/1‑94 (MQ=60)
                         CGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCC                                                                  >  SRR4302534.5963593/1‑94 (MQ=60)
                           AGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGT                                                                >  SRR4302534.5997951/1‑94 (MQ=60)
                            GTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                               <  SRR4302534.4799504/94‑1 (MQ=60)
                            GTGTAGAAAAACCTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTT                                                               <  SRR4302534.5926986/94‑1 (MQ=60)
                             TGTAGTAAAACCTGCCGTAGAAGTCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              <  SRR4302534.1698534/94‑1 (MQ=60)
                             TGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              <  SRR4302534.872049/94‑1 (MQ=60)
                             TGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTT                                                              <  SRR4302534.6509277/94‑1 (MQ=60)
                              gaggtaaaacgtcccgaAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTA                                                             <  SRR4302534.2477977/77‑1 (MQ=60)
                              GTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTA                                                             <  SRR4302534.4141514/94‑1 (MQ=60)
                               TAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAA                                                            <  SRR4302534.857084/94‑1 (MQ=60)
                               TAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAA                                                            <  SRR4302534.5412300/94‑1 (MQ=60)
                                AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                           <  SRR4302534.1413403/94‑1 (MQ=60)
                                AGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAG                                                           >  SRR4302534.1578505/1‑94 (MQ=60)
                                 GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          <  SRR4302534.2164151/94‑1 (MQ=60)
                                 GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          <  SRR4302534.6623906/94‑1 (MQ=60)
                                 GTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGG                                                          <  SRR4302534.6394720/94‑1 (MQ=60)
                                  TAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGG                                                         >  SRR4302534.4202/1‑94 (MQ=60)
                                   AAAAACTGGCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        >  SRR4302534.6531393/1‑94 (MQ=60)
                                   AAAAACTGCCGTAGAAGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        <  SRR4302534.5906124/94‑1 (MQ=60)
                                   AAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGC                                                        >  SRR4302534.4891410/1‑94 (MQ=60)
                                    AAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCG                                                       <  SRR4302534.2993766/94‑1 (MQ=60)
                                     AAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                      <  SRR4302534.668821/94‑1 (MQ=60)
                                     AAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                      >  SRR4302534.1400083/1‑94 (MQ=60)
                                     AAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGA                                                      <  SRR4302534.5200836/94‑1 (MQ=60)
                                       ACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCCGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCAAGCCGGTTAAGGGCGATC                                                    >  SRR4302534.3416990/1‑94 (MQ=60)
                                          GCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATG                                                 <  SRR4302534.5917233/94‑1 (MQ=60)
                                           CTGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGA                                                <  SRR4302534.2733031/94‑1 (MQ=60)
                                           CGGTAGAGGGCGTTTTTTAACGAGCGACCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGA                                                <  SRR4302534.1174194/94‑1 (MQ=60)
                                           CCGTAGAAGGCTGTTTTTTACAAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGA                                                <  SRR4302534.3645898/94‑1 (MQ=60)
                                            CGTAGAAGGCGGTTTTTTACCAGCGTGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAG                                               <  SRR4302534.1430531/94‑1 (MQ=60)
                                             GTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGC                                              <  SRR4302534.1720345/94‑1 (MQ=60)
                                              TAGAGGGCGGTTTTTTACGAGCGCGCACGACGCCGGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGTGCGATCATGAGCA                                             >  SRR4302534.2792935/1‑94 (MQ=60)
                                              TAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                             >  SRR4302534.464441/1‑94 (MQ=60)
                                              TAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                             <  SRR4302534.4797330/94‑1 (MQ=60)
                                              TAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCC                                             >  SRR4302534.1580052/1‑94 (MQ=60)
                                               agagggggtTTTTTTACGACCGACCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            <  SRR4302534.49115/85‑1 (MQ=60)
                                               AGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            >  SRR4302534.1825402/1‑94 (MQ=60)
                                               AGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            >  SRR4302534.5887394/1‑94 (MQ=60)
                                               AGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCA                                            >  SRR4302534.6077027/1‑94 (MQ=60)
                                               AGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGAGCATGAGCCA                                            <  SRR4302534.6449315/94‑1 (MQ=60)
                                                GAGGGCGTTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAG                                           <  SRR4302534.1163292/94‑1 (MQ=60)
                                                GAGGGCGGTTTTTGACGACCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAG                                           <  SRR4302534.4948548/94‑1 (MQ=60)
                                                GAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAG                                           <  SRR4302534.6241236/94‑1 (MQ=60)
                                                GAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAG                                           >  SRR4302534.4852951/1‑94 (MQ=60)
                                                 agggggttttttgaggagcggcCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGGGCCAGA                                          <  SRR4302534.40642/72‑1 (MQ=60)
                                                 AAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGA                                          >  SRR4302534.3847075/1‑94 (MQ=60)
                                                   GGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATA                                        <  SRR4302534.4467971/94‑1 (MQ=60)
                                                    GCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATAT                                       <  SRR4302534.4219005/94‑1 (MQ=60)
                                                     CGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATG                                      >  SRR4302534.626548/1‑94 (MQ=60)
                                                     CGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATG                                      <  SRR4302534.4132282/94‑1 (MQ=60)
                                                      GGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                     <  SRR4302534.859322/94‑1 (MQ=60)
                                                      GGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGA                                     >  SRR4302534.2978335/1‑94 (MQ=60)
                                                       atgttaaacgagcgagctagactctatTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATTAC                                    <  SRR4302534.4124448/67‑1 (MQ=60)
                                                        TTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACC                                   <  SRR4302534.6357476/94‑1 (MQ=60)
                                                         TTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                                  <  SRR4302534.5713778/94‑1 (MQ=60)
                                                         TTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCT                                  <  SRR4302534.1922775/94‑1 (MQ=60)
                                                          tttaagaggaGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTA                                 <  SRR4302534.121040/84‑1 (MQ=60)
                                                           TTAACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTAC                                <  SRR4302534.1500251/94‑1 (MQ=60)
                                                            TTACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACG                               <  SRR4302534.3839081/94‑1 (MQ=60)
                                                              ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCGTGAGCCAGATATGACCTACGTT                             <  SRR4302534.4200875/94‑1 (MQ=60)
                                                              ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTT                             >  SRR4302534.5243116/1‑94 (MQ=60)
                                                              ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTT                             <  SRR4302534.3007609/94‑1 (MQ=60)
                                                              ACGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTT                             <  SRR4302534.2729052/94‑1 (MQ=60)
                                                              ACGAGCGAGCAAGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTT                             <  SRR4302534.309029/94‑1 (MQ=60)
                                                               CGAGCGAGCACGACGCCAGTTGACGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAACGGCGATCATGAGCCAGATATcccctaccata                            >  SRR4302534.2066281/1‑83 (MQ=60)
                                                               CGAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTG                            >  SRR4302534.6060444/1‑94 (MQ=60)
                                                                GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGA                           <  SRR4302534.1827967/94‑1 (MQ=60)
                                                                GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGA                           <  SRR4302534.4310384/94‑1 (MQ=60)
                                                                GAGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGA                           >  SRR4302534.5346432/1‑94 (MQ=60)
                                                                 AGCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAA                          <  SRR4302534.3581636/94‑1 (MQ=60)
                                                                  GCGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAAT                         >  SRR4302534.333004/1‑94 (MQ=60)
                                                                   CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        <  SRR4302534.3187177/94‑1 (MQ=60)
                                                                   CGAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATC                        >  SRR4302534.3293802/1‑94 (MQ=60)
                                                                    GAGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCG                       >  SRR4302534.2386811/1‑94 (MQ=60)
                                                                     AGCACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGG                      >  SRR4302534.462025/1‑94 (MQ=60)
                                                                        ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGGGATCATGAGCCAGATATGACCTACGTTGAATCCGCTG                   >  SRR4302534.877309/1‑94 (MQ=60)
                                                                        ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   <  SRR4302534.860425/94‑1 (MQ=60)
                                                                        ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   <  SRR4302534.2075099/94‑1 (MQ=60)
                                                                        ACGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTG                   >  SRR4302534.4218882/1‑94 (MQ=60)
                                                                         CGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGC                  >  SRR4302534.1573963/1‑94 (MQ=60)
                                                                         CGACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGC                  >  SRR4302534.201897/1‑94 (MQ=60)
                                                                          GACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCT                 >  SRR4302534.3049048/1‑94 (MQ=60)
                                                                           ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                >  SRR4302534.5362383/1‑94 (MQ=60)
                                                                           ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                <  SRR4302534.6431161/94‑1 (MQ=60)
                                                                           ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                >  SRR4302534.5964066/1‑94 (MQ=60)
                                                                           ACGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                <  SRR4302534.5053610/94‑1 (MQ=60)
                                                                           ACGCCAGGTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTC                <  SRR4302534.4138113/94‑1 (MQ=60)
                                                                            CGCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCG               <  SRR4302534.2058362/94‑1 (MQ=60)
                                                                             GCCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGC              >  SRR4302534.4172874/1‑94 (MQ=60)
                                                                             GCCAGTTGAAGCGGATGCCTGGCTGATTGCTGCACGCACGCCGATTAAAGGAGATCATGAGCCAGATATGACCTACGTTGAATCTGCTGCTCGC              >  SRR4302534.3123210/1‑94 (MQ=60)
                                                                              CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCT             <  SRR4302534.3021253/94‑1 (MQ=60)
                                                                              CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCT             >  SRR4302534.5117120/1‑94 (MQ=60)
                                                                              CCAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGATCGCT             >  SRR4302534.5126011/1‑94 (MQ=60)
                                                                               CAGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTC            >  SRR4302534.6087003/1‑94 (MQ=60)
                                                                                AGTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCC           >  SRR4302534.3360211/1‑94 (MQ=60)
                                                                                 GTTGAAGCGGATGCCTGTCTGATTGCTGTACCTACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCA          <  SRR4302534.5731800/94‑1 (MQ=60)
                                                                                 GTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCA          >  SRR4302534.4830458/1‑94 (MQ=60)
                                                                                 GTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCA          <  SRR4302534.2286087/94‑1 (MQ=60)
                                                                                 GTTGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCA          <  SRR4302534.3327765/94‑1 (MQ=60)
                                                                                   TGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT        >  SRR4302534.5721609/1‑94 (MQ=60)
                                                                                   TGAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATT        <  SRR4302534.4362816/94‑1 (MQ=60)
                                                                                    GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       >  SRR4302534.1662009/1‑94 (MQ=60)
                                                                                    GAAGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTG       >  SRR4302534.5949572/1‑94 (MQ=60)
                                                                                      AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     <  SRR4302534.317128/94‑1 (MQ=60)
                                                                                      AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     >  SRR4302534.3099936/1‑94 (MQ=60)
                                                                                      AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     <  SRR4302534.3855731/94‑1 (MQ=60)
                                                                                      AGCGGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCG     <  SRR4302534.2125771/94‑1 (MQ=60)
                                                                                         GGATGCCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  SRR4302534.3533386/1‑94 (MQ=60)
                                                                                         GGATGACTGGCTGATTGCTGTGCCCACGCCGTTTAAGCGCGATCATGAGCCAGATATGACTTACGTTCAATCGCCTGACCGCTCCATTGggcta  >  SRR4302534.5392916/1‑89 (MQ=53)
                                                                                             |                                                                                         
ATCCATATCGTCGAACCTGATTTGGCGAGTGTAGTAAAAACTGCCGTAGAAGGCGGTTTTTTACGAGCGAGCACGACGCCAGTTGAAGCGGATCGCTGGCTGATTGCTGTACCCACGCCGTTTAAGGGCGATCATGAGCCAGATATGACCTACGTTGAATCGGCTGCTCGCTCCATTGCGCCA  >  NC_000913/3969010‑3969192

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑