Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,998,290 +G coding (379/486 nt) b4566 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,998,2891.G99.8% 1862.9 / ‑4.1 538coding (380/486 nt)b4566hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (279/258);  total (279/259)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.66e-01

Only 100 of 592 total aligned reads displayed.

GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT  >  NC_000913/3998197‑3998376
                                                                                             |                                                                                       
gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCa                                                                                          <  2:6410289/93‑1 (MQ=255)
gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCa                                                                                          <  2:2516155/93‑1 (MQ=255)
gACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCAACATAAAACACGCCGCCa                                                                                          >  1:5119404/1‑93 (MQ=255)
 aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                        >  1:965327/1‑93 (MQ=255)
 aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                        >  1:286176/1‑93 (MQ=255)
   tGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCGCCCGCGACATAAAACACGCCGCCA‑gca                                                                                      <  1:6794816/93‑3 (MQ=255)
    gCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggac                                                                                     <  2:4068706/93‑4 (MQ=255)
       ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGa                                                                                   >  2:686805/1‑93 (MQ=255)
       ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGa                                                                                   <  2:5335721/93‑1 (MQ=255)
       ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGa                                                                                   <  2:7734875/93‑1 (MQ=255)
       ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGa                                                                                   >  1:3359082/1‑93 (MQ=255)
        cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAt                                                                                  >  2:7044743/1‑93 (MQ=255)
           aaaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGACCGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaaa                                                                               <  2:4861266/93‑1 (MQ=255)
           aaaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACTATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaaa                                                                               >  1:7739086/1‑93 (MQ=255)
           aaaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaaa                                                                               <  1:1850838/93‑1 (MQ=255)
            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAg                                                                              >  2:6113844/1‑93 (MQ=255)
            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAg                                                                              >  2:3599757/1‑93 (MQ=255)
            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAg                                                                              >  2:3119896/1‑93 (MQ=255)
             aCTGAATCTCTTCACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGa                                                                             >  1:1981451/1‑93 (MQ=255)
              cTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaa                                                                            >  2:8387096/1‑93 (MQ=255)
              cTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaa                                                                            <  1:3859399/93‑1 (MQ=255)
               tGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaaa                                                                           >  1:1245775/1‑93 (MQ=255)
               tGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaaa                                                                           >  2:2930201/1‑93 (MQ=255)
                gAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAt                                                                          >  1:4159417/1‑93 (MQ=255)
                gAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAt                                                                          >  2:5492757/1‑93 (MQ=255)
                 aaTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAtt                                                                         >  1:1340166/1‑93 (MQ=255)
                    ctctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTccc                                                                      >  1:7643244/1‑93 (MQ=255)
                    ctctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTccc                                                                      <  1:2417081/93‑1 (MQ=255)
                     tctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCt                                                                     >  2:7107558/1‑93 (MQ=255)
                     tctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCt                                                                     >  1:5673677/1‑93 (MQ=255)
                      ctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCtt                                                                    >  2:7909680/1‑93 (MQ=255)
                       tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTc                                                                   <  2:542949/93‑1 (MQ=255)
                       tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTc                                                                   >  2:2210851/1‑93 (MQ=255)
                        ttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCa                                                                  >  2:6623179/1‑93 (MQ=255)
                          aCTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATTTCCCGCGACATAAAACACGCCGCCAGGACGGTTAAAGACATTCCCTTAAcc                                                                >  2:4549470/1‑93 (MQ=255)
                           cTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCt                                                               >  2:7201104/1‑93 (MQ=255)
                            tGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTg                                                              <  2:827941/93‑1 (MQ=255)
                            tGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTg                                                              >  1:8007936/1‑93 (MQ=255)
                              gTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGaa                                                            >  1:6555414/1‑93 (MQ=255)
                               ttttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAg                                                           <  2:5959177/93‑1 (MQ=255)
                                tttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGc                                                          <  1:5266449/93‑1 (MQ=255)
                                 ttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCg                                                         <  2:3742553/93‑1 (MQ=255)
                                 ttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCg                                                         <  2:895284/93‑1 (MQ=255)
                                 ttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCg                                                         >  2:8010729/1‑93 (MQ=255)
                                  tGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCga                                                        <  1:7112012/93‑1 (MQ=255)
                                   gAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgag                                                       <  2:3837943/93‑1 (MQ=255)
                                    aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgaga                                                      >  2:6219820/1‑93 (MQ=255)
                                         aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTaa                                                 >  2:4255630/1‑93 (MQ=255)
                                          tCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAc                                                >  2:2718761/1‑93 (MQ=255)
                                           ccAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACg                                               >  1:8363/1‑93 (MQ=255)
                                             aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGTGAGATTTAACGcc                                             >  2:3517321/1‑93 (MQ=255)
                                             aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGcc                                             <  1:4002784/93‑1 (MQ=255)
                                              gACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCa                                            >  2:5036657/1‑93 (MQ=255)
                                               acgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCGCCTGAAGCGAGATTTAACGCCAc                                           <  2:1137661/93‑1 (MQ=255)
                                                cgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAcc                                          <  2:577727/93‑1 (MQ=255)
                                                  aacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCtt                                        <  1:4649008/93‑1 (MQ=255)
                                                  aacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCtt                                        >  2:5805764/1‑93 (MQ=255)
                                                   acgaTGCCGACTTTATCTCCCGCGACATAAAACTCGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTc                                       <  1:8515259/93‑1 (MQ=255)
                                                    cgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTct                                      <  1:2776851/93‑1 (MQ=255)
                                                     gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGCAGCGAGATTTAACGCCACCTTctg                                     >  2:7332957/1‑93 (MQ=255)
                                                     gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctg                                     >  2:4629051/1‑93 (MQ=255)
                                                     gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctg                                     >  2:4441893/1‑93 (MQ=255)
                                                     gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctg                                     >  1:7393565/1‑93 (MQ=255)
                                                      aTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                    >  1:1755167/1‑93 (MQ=255)
                                                       tGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgct                                   >  1:3669262/1‑93 (MQ=255)
                                                         ccGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGa                                 <  1:62011/93‑1 (MQ=255)
                                                           gACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGatc                               >  2:175953/1‑91 (MQ=255)
                                                           gACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAcc                               >  1:5970099/1‑93 (MQ=255)
                                                           gACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAcc                               >  1:754626/1‑93 (MQ=255)
                                                             cTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGt                             >  1:100364/1‑93 (MQ=255)
                                                             cTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGt                             <  1:3477106/93‑1 (MQ=255)
                                                              tttATCTCCCGCGCCCTCAGACACACCGCCAGGACGGATAAAGAAATTCCCTTCACCTGATGCAAGATTTAACGCCACCTTCTGCTGACCGtt                            <  1:7828892/93‑1 (MQ=255)
                                                               ttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGttt                           <  2:346889/93‑1 (MQ=255)
                                                               ttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGttt                           <  1:2252262/93‑1 (MQ=255)
                                                                tATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtttt                          <  2:7463229/93‑1 (MQ=255)
                                                                tATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtttt                          <  2:570208/93‑1 (MQ=255)
                                                                 aTCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTc                         <  2:1198178/93‑1 (MQ=255)
                                                                 aTCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTc                         <  1:5854290/93‑1 (MQ=255)
                                                                  tctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCg                        >  2:6990632/1‑93 (MQ=255)
                                                                  tctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCg                        >  1:8658357/1‑93 (MQ=255)
                                                                   ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGa                       <  1:4319671/93‑1 (MQ=255)
                                                                   ctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGa                       >  1:7454534/1‑93 (MQ=255)
                                                                    tcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAt                      <  1:3074968/93‑1 (MQ=255)
                                                                     cccGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATg                     >  2:1590752/1‑93 (MQ=255)
                                                                     cccGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATg                     >  1:1552830/1‑93 (MQ=255)
                                                                      ccGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGt                    <  2:5156547/93‑1 (MQ=255)
                                                                      ccGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGt                    >  2:2116867/1‑93 (MQ=255)
                                                                        gcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCCCCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAc                  <  2:1392007/93‑1 (MQ=255)
                                                                        gcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAc                  >  1:5384065/1‑93 (MQ=255)
                                                                           aCATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaaa               >  1:6969397/1‑93 (MQ=255)
                                                                            cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAc              <  2:8594215/93‑1 (MQ=255)
                                                                            cATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAc              >  1:4814348/1‑93 (MQ=255)
                                                                             aTAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGCTTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc             >  1:4475477/1‑93 (MQ=255)
                                                                             aTAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc             <  2:3342013/93‑1 (MQ=255)
                                                                              tAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCg            >  1:1078276/1‑93 (MQ=255)
                                                                              tAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCg            >  1:5546671/1‑93 (MQ=255)
                                                                                 aaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgc         >  1:2118155/1‑93 (MQ=255)
                                                                                    acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggc      <  2:1881994/93‑1 (MQ=255)
                                                                                       ccgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCa   >  1:1463261/1‑93 (MQ=255)
                                                                                        cgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAt  <  1:4959347/93‑1 (MQ=255)
                                                                                             |                                                                                       
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT  >  NC_000913/3998197‑3998376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 326 total aligned reads displayed.
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  >  NC_000913/3998197‑3998382
                                                                                               |                                                                                            
gacgaCACCGCAAACTGACTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATCCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CA‑G                                                                                              <  SRR4302541.2243037/89‑1 (MQ=60)
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CA‑G                                                                                              >  SRR4302541.288625/1‑94 (MQ=60)
 ACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CA‑GG                                                                                             <  SRR4302541.5390873/94‑1 (MQ=60)
 ACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CA‑GG                                                                                             <  SRR4302541.1828903/94‑1 (MQ=60)
 ACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CA‑GG                                                                                             <  SRR4302541.1976672/94‑1 (MQ=60)
  CTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CA‑Gga                                                                                            <  SRR4302541.137539/94‑3 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CA‑Ggac                                                                                           <  SRR4302541.8719632/94‑4 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CA‑Ggac                                                                                           <  SRR4302541.7653616/94‑4 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CA‑Ggac                                                                                           <  SRR4302541.5902596/94‑4 (MQ=60)
    GCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACG                                                                                           <  SRR4302541.7186332/94‑1 (MQ=60)
    GCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACG                                                                                           <  SRR4302541.726585/94‑1 (MQ=60)
    GCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACG                                                                                           <  SRR4302541.1934118/94‑1 (MQ=60)
     CACCTCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGG                                                                                          <  SRR4302541.1543849/94‑1 (MQ=60)
     CACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGG                                                                                          >  SRR4302541.5658448/1‑94 (MQ=60)
     CACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGG                                                                                          <  SRR4302541.1543850/94‑1 (MQ=60)
      ACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGA                                                                                         <  SRR4302541.6645764/94‑1 (MQ=60)
      ACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGA                                                                                         >  SRR4302541.3392602/1‑94 (MQ=60)
       CCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGAT                                                                                        <  SRR4302541.1408918/94‑1 (MQ=60)
       CCGCAAACTGAATCTCTTTACTGGTTGTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGAT                                                                                        <  SRR4302541.7262586/94‑1 (MQ=60)
        CGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAACCACGCCGC‑CAGGACGGATA                                                                                       >  SRR4302541.1004702/1‑94 (MQ=60)
         GCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAA                                                                                      >  SRR4302541.1763896/1‑94 (MQ=60)
          CAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCCGACGAACGATGCCGACTTTATCTCCCGCGACATAACACCCGCCGC‑CAGGACGGATAAA                                                                                     >  SRR4302541.8567713/1‑94 (MQ=60)
          CAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACTATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAA                                                                                     >  SRR4302541.7821422/1‑94 (MQ=60)
          CAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACTATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAA                                                                                     >  SRR4302541.7821423/1‑94 (MQ=60)
          CAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAA                                                                                     >  SRR4302541.5076755/1‑94 (MQ=60)
          CAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAA                                                                                     >  SRR4302541.5914956/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAG                                                                                    <  SRR4302541.4808183/94‑1 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAG                                                                                    <  SRR4302541.8122053/94‑1 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAG                                                                                    <  SRR4302541.3775889/94‑1 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAG                                                                                    >  SRR4302541.789079/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAG                                                                                    <  SRR4302541.6011403/94‑1 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCACGACATAAAACACGCCGC‑CAGGACGGATAAAG                                                                                    >  SRR4302541.7766426/1‑94 (MQ=60)
            AACTGAATCTCTTCACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGA                                                                                   >  SRR4302541.2000597/1‑94 (MQ=60)
             ACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAA                                                                                  <  SRR4302541.8665693/94‑1 (MQ=60)
              CTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAA                                                                                 <  SRR4302541.3898224/94‑1 (MQ=60)
              CTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAA                                                                                 >  SRR4302541.7491327/1‑94 (MQ=60)
              CTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAA                                                                                 >  SRR4302541.7837397/1‑94 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGAGGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAAT                                                                                <  SRR4302541.1635463/94‑1 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAAT                                                                                <  SRR4302541.5990179/94‑1 (MQ=60)
                GAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATT                                                                               <  SRR4302541.1618700/94‑1 (MQ=60)
                   TCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCC                                                                            >  SRR4302541.7724716/1‑94 (MQ=60)
                   TCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCC                                                                            <  SRR4302541.5261450/94‑1 (MQ=60)
                    CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCT                                                                           <  SRR4302541.2440898/94‑1 (MQ=60)
                    CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCT                                                                           >  SRR4302541.5731581/1‑94 (MQ=60)
                     TCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTT                                                                          <  SRR4302541.4042935/94‑1 (MQ=60)
                     TCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTT                                                                          <  SRR4302541.558810/94‑1 (MQ=60)
                      CTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTC                                                                         >  SRR4302541.7388395/1‑94 (MQ=60)
                       TTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCA                                                                        >  SRR4302541.7661419/1‑94 (MQ=60)
                          ACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCT                                                                     >  SRR4302541.2170288/1‑94 (MQ=60)
                          ACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCT                                                                     >  SRR4302541.4248253/1‑94 (MQ=60)
                          ACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCT                                                                     >  SRR4302541.4851534/1‑94 (MQ=60)
                           CTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTG                                                                    >  SRR4302541.6407277/1‑94 (MQ=60)
                           CTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTG                                                                    >  SRR4302541.8096018/1‑94 (MQ=60)
                           CTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACAAGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTG                                                                    >  SRR4302541.6091684/1‑94 (MQ=60)
                            TGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGA                                                                   <  SRR4302541.8244758/94‑1 (MQ=60)
                             GGTTTTGAAGGCCTCCAGCCGAACAATGCCGCCTTTATCTCCCGCGACATAAACCACGCCCC‑CAGGACCGATAAAGAAATTCCCTTCACCTGAA                                                                  >  SRR4302541.6622419/1‑94 (MQ=57)
                             GGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                  >  SRR4302541.6622418/1‑94 (MQ=60)
                             GGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                  <  SRR4302541.5230040/94‑1 (MQ=60)
                             GGTTTTGAAGGCATCCAGACGAACGATACCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                  <  SRR4302541.6783904/94‑1 (MQ=60)
                               TTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGC                                                                >  SRR4302541.5520690/1‑94 (MQ=60)
                                TTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGACGCG                                                               >  SRR4302541.1229512/1‑94 (MQ=60)
                                TTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCG                                                               <  SRR4302541.5320716/94‑1 (MQ=60)
                                 TTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGA                                                              <  SRR4302541.301587/94‑1 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                             <  SRR4302541.7187757/94‑1 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                             >  SRR4302541.1040266/1‑94 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                             <  SRR4302541.4443348/94‑1 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                             <  SRR4302541.4443349/94‑1 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTGTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                             <  SRR4302541.3682026/94‑1 (MQ=60)
                                   GAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAACCACGCCGCACG‑GAGGGCTCAAGAAATTCCCTTCACCCGAAGCGcgt                                                            >  SRR4302541.3976594/1‑91 (MQ=60)
                                   GAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGA                                                            <  SRR4302541.2819421/94‑1 (MQ=60)
                                   GAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGA                                                            <  SRR4302541.1375952/94‑1 (MQ=60)
                                    AAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAT                                                           <  SRR4302541.2698315/94‑1 (MQ=60)
                                    AAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAT                                                           <  SRR4302541.4626527/94‑1 (MQ=60)
                                     AGGCAACCAGACGAACGATGCCGACTTTATCGCCCTCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATT                                                          <  SRR4302541.5217728/94‑1 (MQ=60)
                                        CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAA                                                       <  SRR4302541.8345557/94‑1 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      >  SRR4302541.6253998/1‑94 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      >  SRR4302541.2057386/1‑94 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      <  SRR4302541.7990177/94‑1 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCCCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      <  SRR4302541.7567642/94‑1 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTGATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                      <  SRR4302541.2107201/94‑1 (MQ=60)
                                           CCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGC                                                    >  SRR4302541.1860087/1‑94 (MQ=60)
                                           CCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGC                                                    >  SRR4302541.4819738/1‑94 (MQ=60)
                                           CCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGC                                                    >  SRR4302541.5736049/1‑94 (MQ=60)
                                            CAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCC                                                   >  SRR4302541.1516526/1‑94 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                  <  SRR4302541.4042399/94‑1 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                  >  SRR4302541.3708130/1‑94 (MQ=60)
                                             AGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA                                                  >  SRR4302541.1866761/1‑94 (MQ=60)
                                              GACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAC                                                 >  SRR4302541.4106400/1‑94 (MQ=60)
                                                CGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                               <  SRR4302541.5052223/94‑1 (MQ=60)
                                                 GAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                              >  SRR4302541.4225557/1‑94 (MQ=60)
                                                 GAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                              >  SRR4302541.3315469/1‑94 (MQ=60)
                                                  AACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                             <  SRR4302541.4696346/94‑1 (MQ=60)
                                                  AACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                             >  SRR4302541.3330154/1‑94 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACTCGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            <  SRR4302541.8606918/94‑1 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGCAGCGAGATTTAACGCCACCTTCT                                            >  SRR4302541.2326375/1‑94 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            <  SRR4302541.125604/94‑1 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            <  SRR4302541.1970151/94‑1 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            >  SRR4302541.1267452/1‑94 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            <  SRR4302541.1843992/94‑1 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            <  SRR4302541.7177705/94‑1 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTCCT                                            >  SRR4302541.3749378/1‑94 (MQ=60)
                                                   ACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATGCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            <  SRR4302541.8797765/94‑1 (MQ=60)
                                                   ACGATGCCCACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCT                                            <  SRR4302541.5446813/94‑1 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           >  SRR4302541.7472627/1‑94 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           <  SRR4302541.2804829/94‑1 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           <  SRR4302541.2515154/94‑1 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                           <  SRR4302541.411043/94‑1 (MQ=60)
                                                      ATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCT                                         <  SRR4302541.340692/94‑1 (MQ=60)
                                                      ATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCT                                         <  SRR4302541.7958276/94‑1 (MQ=60)
                                                       TGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGTGAGATTTAACGCCACCTTCTGCTG                                        <  SRR4302541.3552704/94‑1 (MQ=60)
                                                         CCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAC                                      <  SRR4302541.62511/94‑1 (MQ=60)
                                                          CGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACC                                     <  SRR4302541.7576978/94‑1 (MQ=60)
                                                          CGACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACC                                     >  SRR4302541.2290301/1‑94 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    >  SRR4302541.3466933/1‑94 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                    >  SRR4302541.6876048/1‑94 (MQ=60)
                                                            ACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                   >  SRR4302541.990060/1‑94 (MQ=60)
                                                            ACTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                   >  SRR4302541.101215/1‑94 (MQ=60)
                                                             CTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTT                                  >  SRR4302541.1103002/1‑94 (MQ=60)
                                                             CTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTT                                  >  SRR4302541.2121989/1‑94 (MQ=60)
                                                             CTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTT                                  >  SRR4302541.2258080/1‑94 (MQ=60)
                                                             CTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTT                                  >  SRR4302541.3507172/1‑94 (MQ=60)
                                                             CTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTT                                  <  SRR4302541.3511884/94‑1 (MQ=60)
                                                             CTTTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTT                                  >  SRR4302541.6425591/1‑94 (MQ=60)
                                                               TTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTT                                <  SRR4302541.3110118/94‑1 (MQ=60)
                                                               TTATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTT                                <  SRR4302541.798335/94‑1 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                               <  SRR4302541.5670855/94‑1 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                               >  SRR4302541.306626/1‑94 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                               >  SRR4302541.6660095/1‑94 (MQ=60)
                                                                 ATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                              <  SRR4302541.8861991/94‑1 (MQ=60)
                                                                 ATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                              <  SRR4302541.5913822/94‑1 (MQ=60)
                                                                 ATCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                              >  SRR4302541.8751043/1‑94 (MQ=60)
                                                                  TCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGA                             >  SRR4302541.7534143/1‑94 (MQ=60)
                                                                  TCTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGA                             <  SRR4302541.813306/94‑1 (MQ=60)
                                                                   CTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAT                            >  SRR4302541.1068674/1‑94 (MQ=60)
                                                                   CTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAT                            <  SRR4302541.4362805/94‑1 (MQ=60)
                                                                   CTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAT                            >  SRR4302541.3940646/1‑94 (MQ=60)
                                                                   CTCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAT                            <  SRR4302541.1153854/94‑1 (MQ=60)
                                                                    TCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAATAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATG                           <  SRR4302541.3106163/94‑1 (MQ=60)
                                                                    TCCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATG                           >  SRR4302541.4928369/1‑94 (MQ=60)
                                                                     CCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTTACCGTTTTCGATGT                          >  SRR4302541.7067870/1‑94 (MQ=60)
                                                                     CCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGT                          <  SRR4302541.3992583/94‑1 (MQ=60)
                                                                     CCCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAACTTCTGCTGACCGTTTTCGATGT                          <  SRR4302541.2380221/94‑1 (MQ=60)
                                                                      CCGCGACATAAAACACGCCGC‑CAGGGCGCGTAAAGAAATTCCCTTCACCGGAAGCGAGATTTAACGCCACCTTCTGCTGACTGTTTTGGTTGTA                         >  SRR4302541.4571683/1‑94 (MQ=60)
                                                                      CCGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTA                         <  SRR4302541.7733560/94‑1 (MQ=60)
                                                                       CGCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAC                        >  SRR4302541.5439334/1‑94 (MQ=60)
                                                                        GCGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACA                       <  SRR4302541.6935034/94‑1 (MQ=60)
                                                                         CGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                      <  SRR4302541.8653844/94‑1 (MQ=60)
                                                                         CGACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCA‑CTTCTGCCGACCGTTTTTGATGTACAAA                     >  SRR4302541.3704585/1‑94 (MQ=60)
                                                                          GACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                     >  SRR4302541.7526058/1‑94 (MQ=60)
                                                                          GACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                     <  SRR4302541.7010347/94‑1 (MQ=60)
                                                                          GACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                     >  SRR4302541.7043672/1‑94 (MQ=60)
                                                                          GACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                     >  SRR4302541.5610160/1‑94 (MQ=60)
                                                                          GACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                     >  SRR4302541.6056810/1‑94 (MQ=60)
                                                                           ACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                    >  SRR4302541.2880537/1‑94 (MQ=60)
                                                                           ACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                    >  SRR4302541.4863437/1‑94 (MQ=60)
                                                                           ACATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                    >  SRR4302541.2372314/1‑94 (MQ=60)
                                                                            CATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACG                   >  SRR4302541.5409303/1‑94 (MQ=60)
                                                                            CATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACC                   >  SRR4302541.4520580/1‑94 (MQ=60)
                                                                             ATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                  >  SRR4302541.3024787/1‑94 (MQ=60)
                                                                             ATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                  >  SRR4302541.8544338/1‑94 (MQ=60)
                                                                             ATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                  >  SRR4302541.5603460/1‑94 (MQ=60)
                                                                             ATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                  >  SRR4302541.6116262/1‑94 (MQ=60)
                                                                             ATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                  <  SRR4302541.4366388/94‑1 (MQ=60)
                                                                             ATAAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                  >  SRR4302541.1088325/1‑94 (MQ=60)
                                                                               AAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACTCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAG                <  SRR4302541.8493605/94‑1 (MQ=60)
                                                                               AAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAG                >  SRR4302541.4885532/1‑94 (MQ=60)
                                                                               AAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAG                <  SRR4302541.1259666/94‑1 (MQ=60)
                                                                               AAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAG                >  SRR4302541.8864052/1‑94 (MQ=60)
                                                                               AAAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAG                <  SRR4302541.748656/94‑1 (MQ=60)
                                                                                AAACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGC               >  SRR4302541.2138665/1‑94 (MQ=60)
                                                                                 AACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG              <  SRR4302541.6286153/94‑1 (MQ=60)
                                                                                 AACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG              <  SRR4302541.426476/94‑1 (MQ=60)
                                                                                 AACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG              >  SRR4302541.1295357/1‑94 (MQ=60)
                                                                                 AACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG              <  SRR4302541.7603325/94‑1 (MQ=60)
                                                                                 AACACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG              <  SRR4302541.1770943/94‑1 (MQ=60)
                                                                                   CACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGC            <  SRR4302541.4906040/94‑1 (MQ=60)
                                                                                   CACGCCGC‑CAGGACGAATACAGCTATTCCCTTCGCCTGAAGCGAGATTTAACGCCACCTTATGATGACCGTTTTCGATGTACCAACCGAGCGGC            <  SRR4302541.7898848/94‑1 (MQ=55)
                                                                                    ACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCG           >  SRR4302541.763464/1‑94 (MQ=60)
                                                                                    ACGCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCG           >  SRR4302541.5247441/1‑94 (MQ=60)
                                                                                      GCCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCA         >  SRR4302541.1477592/1‑94 (MQ=60)
                                                                                       CCGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT        >  SRR4302541.582198/1‑94 (MQ=60)
                                                                                        CGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA       <  SRR4302541.5010100/94‑1 (MQ=60)
                                                                                        CGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA       <  SRR4302541.4642340/94‑1 (MQ=60)
                                                                                        CGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA       >  SRR4302541.3964822/1‑94 (MQ=60)
                                                                                        CGC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA       >  SRR4302541.3898279/1‑94 (MQ=60)
                                                                                         GC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCCCCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAG      <  SRR4302541.4146348/94‑1 (MQ=60)
                                                                                         GC‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAG      <  SRR4302541.7389075/94‑1 (MQ=60)
                                                                                          C‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGC     >  SRR4302541.6413756/1‑94 (MQ=60)
                                                                                          C‑CAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGC     >  SRR4302541.1710143/1‑94 (MQ=60)
                                                                                            cagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCT    >  SRR4302541.2230879/4‑94 (MQ=60)
                                                                                            cagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCT    >  SRR4302541.177543/4‑94 (MQ=60)
                                                                                            cagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCT    >  SRR4302541.1568025/4‑94 (MQ=60)
                                                                                             agGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT   <  SRR4302541.2808730/92‑1 (MQ=60)
                                                                                             agGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT   >  SRR4302541.2621786/3‑94 (MQ=60)
                                                                                             agGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT   >  SRR4302541.4452868/3‑94 (MQ=60)
                                                                                             G‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  >  SRR4302541.3170077/1‑94 (MQ=60)
                                                                                             G‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  >  SRR4302541.7042745/1‑94 (MQ=60)
                                                                                             G‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  >  SRR4302541.455175/1‑94 (MQ=60)
                                                                                             G‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  >  SRR4302541.1047602/1‑94 (MQ=60)
                                                                                             G‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  <  SRR4302541.5437296/94‑1 (MQ=60)
                                                                                               |                                                                                            
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGC‑CA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTT  >  NC_000913/3998197‑3998382

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑