Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,998,290 +G coding (379/486 nt) b4566 ← hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,998,2891.G99.5% 715.9 / ‑3.5 207coding (380/486 nt)b4566hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base G (118/88);  total (119/88)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.34e-01

Only 100 of 226 total aligned reads displayed.

GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT  >  NC_000913/3998197‑3998376
                                                                                             |                                                                                       
gACTGCGCCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCa                                                                                          <  1:5914669/93‑1 (MQ=255)
 aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGACCGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                        <  2:3446811/93‑1 (MQ=255)
 aCTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑g                                                                                        <  2:6345434/93‑1 (MQ=255)
  cTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑gg                                                                                       >  2:5022591/1‑92 (MQ=255)
  cTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑gg                                                                                       <  2:2380807/93‑2 (MQ=255)
    gCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑ggac                                                                                     >  1:1960755/1‑90 (MQ=255)
       ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGa                                                                                   >  2:5544730/1‑93 (MQ=255)
       ccGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGa                                                                                   >  2:5350332/1‑93 (MQ=255)
        cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAt                                                                                  >  1:5678143/1‑93 (MQ=255)
        cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAt                                                                                  >  1:742253/1‑93 (MQ=255)
        cGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAt                                                                                  >  2:3385915/1‑93 (MQ=255)
          cAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATaa                                                                                >  1:6341408/1‑93 (MQ=255)
            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAg                                                                              >  1:1019933/1‑93 (MQ=255)
            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAg                                                                              >  2:3472038/1‑93 (MQ=255)
            aaCTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAg                                                                              >  1:1198500/1‑93 (MQ=255)
              cTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaa                                                                            >  1:5747215/1‑93 (MQ=255)
              cTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaa                                                                            >  1:3135548/1‑93 (MQ=255)
              cTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaa                                                                            >  2:5418133/1‑93 (MQ=255)
               tGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaaa                                                                           >  1:4925159/1‑93 (MQ=255)
               tGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGaaa                                                                           >  2:1398793/1‑93 (MQ=255)
                 aaTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAtt                                                                         >  2:284285/1‑93 (MQ=255)
                 aaTCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAtt                                                                         >  1:2189131/1‑93 (MQ=255)
                   tctcTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTcc                                                                       >  1:2278339/1‑93 (MQ=255)
                   tctcTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTcc                                                                       >  2:1955075/1‑93 (MQ=255)
                    ctctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTccc                                                                      <  2:3781805/93‑1 (MQ=255)
                    ctctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTccc                                                                      >  1:1157283/1‑93 (MQ=255)
                    ctctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTccc                                                                      >  1:2505795/1‑93 (MQ=255)
                     tctTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACCTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCt                                                                     >  2:1975368/1‑93 (MQ=255)
                       tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTc                                                                   >  2:1286081/1‑93 (MQ=255)
                       tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTc                                                                   >  1:3601270/1‑93 (MQ=255)
                       tttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTc                                                                   <  1:530546/93‑1 (MQ=255)
                        ttACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCa                                                                  <  1:2325337/93‑1 (MQ=255)
                            tGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTg                                                              <  2:4150579/93‑1 (MQ=255)
                            tGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTg                                                              >  2:825160/1‑93 (MQ=255)
                             ggTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGa                                                             <  1:4669444/93‑1 (MQ=255)
                             ggTGTTGAAGGCATCCATACGAACGATGCCGACTTTATCGCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGa                                                             <  1:3060390/93‑1 (MQ=255)
                              gTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGaa                                                            >  2:1017101/1‑93 (MQ=255)
                               ttttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAg                                                           <  2:6160382/93‑1 (MQ=255)
                                tttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGc                                                          <  1:53616/93‑1 (MQ=255)
                                 ttGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCAACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCg                                                         >  2:5899333/1‑93 (MQ=255)
                                  tGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCga                                                        <  2:6418064/93‑1 (MQ=255)
                                   gAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCACTTCACCTGAAGCgag                                                       >  1:200513/1‑93 (MQ=255)
                                    aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgaga                                                      >  1:5142601/1‑93 (MQ=255)
                                    aaGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCgaga                                                      <  2:3057534/93‑1 (MQ=255)
                                      ggCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAtt                                                    >  2:3819759/1‑93 (MQ=255)
                                        cATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTa                                                  >  1:817140/1‑93 (MQ=255)
                                         aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTaa                                                 >  1:4020407/1‑93 (MQ=255)
                                         aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTaa                                                 <  2:5009267/93‑1 (MQ=255)
                                         aTCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTaa                                                 >  2:251031/1‑93 (MQ=255)
                                          tCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAc                                                >  1:1634236/1‑93 (MQ=255)
                                             aGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGcc                                             >  2:2218777/1‑93 (MQ=255)
                                              gACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCa                                            >  2:4287622/1‑93 (MQ=255)
                                              gACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCa                                            >  2:6169608/1‑93 (MQ=255)
                                               acgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAc                                           <  2:4118422/93‑1 (MQ=255)
                                               acgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAc                                           >  1:895355/1‑93 (MQ=255)
                                               acgaacgaTGCCGACTTTACCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAc                                           <  2:3730342/93‑1 (MQ=255)
                                                cgaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAcc                                          <  1:984724/93‑1 (MQ=255)
                                                 gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCt                                         >  1:5884950/1‑93 (MQ=255)
                                                 gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCt                                         <  2:4778685/93‑1 (MQ=255)
                                                 gaacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCt                                         <  2:4582895/93‑1 (MQ=255)
                                                 caccgaGGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCt                                         <  1:1394541/90‑1 (MQ=255)
                                                  aacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCtt                                        >  2:5199274/1‑93 (MQ=255)
                                                  aacgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCtt                                        >  1:4758094/1‑93 (MQ=255)
                                                   acgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTc                                       >  1:5771825/1‑93 (MQ=255)
                                                   acgaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTc                                       <  2:298539/93‑1 (MQ=255)
                                                     gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctg                                     >  2:2097140/1‑93 (MQ=255)
                                                     gaTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctg                                     >  1:4912267/1‑93 (MQ=255)
                                                      aTGCGGACTTTATCTCCCGCAACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                    <  1:4428613/93‑1 (MQ=255)
                                                      aTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                    >  1:1675652/1‑93 (MQ=255)
                                                      aTGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTctgc                                    <  2:5759152/93‑1 (MQ=255)
                                                           gACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGAcc                               <  2:734397/93‑1 (MQ=255)
                                                            aCTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCg                              >  1:3761892/1‑93 (MQ=255)
                                                              tttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtt                            <  1:4619821/93‑1 (MQ=255)
                                                              tttATCTCCCGCGACATAAAACACGCCGCCA‑GGCGGATAAAGAAATTCCCTTCAACTGAAGCGAGATTTAACGCCACCCTCTGCTGACCGttt                           >  2:6005468/1‑93 (MQ=255)
                                                               ttATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGttt                           <  1:5205993/93‑1 (MQ=255)
                                                                tATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtttt                          <  1:3018697/93‑1 (MQ=255)
                                                                tATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGtttt                          <  2:3176299/93‑1 (MQ=255)
                                                                 aTCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTc                         >  1:6425678/1‑93 (MQ=255)
                                                                 aTCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTc                         <  1:1581494/93‑1 (MQ=255)
                                                                  tctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCg                        <  2:5694344/93‑1 (MQ=255)
                                                                  tctcCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCg                        <  2:2630739/93‑1 (MQ=255)
                                                                   ctcCCGCGACATAAAACACGCCGCCAGGACGGATAATGAAATTCACTTCGCCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGa                       <  2:6471779/93‑1 (MQ=255)
                                                                        gcgACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTAc                  <  1:2208004/93‑1 (MQ=255)
                                                                          gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaa                >  2:4498198/1‑93 (MQ=255)
                                                                          gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaa                >  1:3530907/1‑93 (MQ=255)
                                                                          gACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaa                >  2:3078308/1‑93 (MQ=255)
                                                                           aCATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACaaa               >  1:1098111/1‑93 (MQ=255)
                                                                             aTAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAcc             <  1:5446001/93‑1 (MQ=255)
                                                                              tAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCg            >  2:1757488/1‑93 (MQ=255)
                                                                              tAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCg            >  1:4294913/1‑93 (MQ=255)
                                                                                aaaCACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAg          <  2:1857985/93‑1 (MQ=255)
                                                                                  acacGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcg        >  2:2800006/1‑93 (MQ=255)
                                                                                  acacGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcg        >  1:3415268/1‑93 (MQ=255)
                                                                                    acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggc      <  2:1641605/93‑1 (MQ=255)
                                                                                    acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggc      <  2:1541971/93‑1 (MQ=255)
                                                                                    acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAgcggc      >  1:4083656/1‑93 (MQ=255)
                                                                                    acGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGCTTTCGATGTACAAACCGAgcggc      >  2:3647545/1‑93 (MQ=255)
                                                                                      gccgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGgcgc    <  1:3266985/93‑1 (MQ=255)
                                                                                       ccgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCa   >  1:2824798/1‑93 (MQ=255)
                                                                                        cgccAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAt  <  2:2723683/93‑1 (MQ=255)
                                                                                             |                                                                                       
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCAT  >  NC_000913/3998197‑3998376

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTC  >  NC_000913/3998197‑3998383
                                                                                              |                                                                                             
GACTGCGCCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                               <  SRR4302534.6075282/94‑1 (MQ=60)
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                               >  SRR4302534.6072335/1‑94 (MQ=60)
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                               >  SRR4302534.5486906/1‑94 (MQ=60)
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑G                                                                                               <  SRR4302534.6580759/94‑1 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                            >  SRR4302534.2012076/1‑91 (MQ=60)
   TGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑Ggac                                                                                            <  SRR4302534.585006/94‑4 (MQ=60)
    GCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACG                                                                                            >  SRR4302534.863017/1‑94 (MQ=60)
     GACCGCAAACTGAATCTCTTTACTGGTTGTGAAGGCATCCAGTCGAACGATGCAGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGG                                                                                           <  SRR4302534.578464/94‑1 (MQ=60)
     CACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGG                                                                                           >  SRR4302534.3294857/1‑94 (MQ=60)
     CACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGG                                                                                           <  SRR4302534.1180126/94‑1 (MQ=60)
      ACCGCAAACTAAATCTCTTTACTGGTTTTGAAGGCATCCAGACTAGCGATGCCGACTTTATCTCCCGCGGCATAAAACACGCCGCCAGGACGGA                                                                                          <  SRR4302534.5320084/94‑1 (MQ=60)
       CCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAT                                                                                         >  SRR4302534.760643/1‑94 (MQ=60)
       CCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAT                                                                                         >  SRR4302534.5831875/1‑94 (MQ=60)
       CCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGAT                                                                                         >  SRR4302534.5372621/1‑94 (MQ=60)
       CCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTACCGCGACATAAAACACGCCGCCAGGACGGAT                                                                                         >  SRR4302534.2047255/1‑94 (MQ=60)
        CGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATA                                                                                        <  SRR4302534.3725693/94‑1 (MQ=60)
         GCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAA                                                                                       >  SRR4302534.6512676/1‑94 (MQ=60)
          CAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAA                                                                                      >  SRR4302534.2378408/1‑94 (MQ=60)
          CAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAA                                                                                      <  SRR4302534.4482253/94‑1 (MQ=60)
          CAAACTGAATCTCTTTACTGGTTTTGAAGGCAGCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAA                                                                                      >  SRR4302534.595697/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAG                                                                                     >  SRR4302534.1046187/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAG                                                                                     >  SRR4302534.3140213/1‑94 (MQ=60)
           AAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAG                                                                                     >  SRR4302534.1230034/1‑94 (MQ=60)
            AACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGA                                                                                    >  SRR4302534.867927/1‑94 (MQ=60)
             ACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAA                                                                                   >  SRR4302534.3218005/1‑94 (MQ=60)
             ACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAA                                                                                   >  SRR4302534.5903049/1‑94 (MQ=60)
              CTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAA                                                                                  >  SRR4302534.5058636/1‑94 (MQ=60)
               TGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAT                                                                                 >  SRR4302534.2298711/1‑94 (MQ=60)
               TGAATCTCTTTACTGGCTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAAT                                                                                 >  SRR4302534.1630465/1‑94 (MQ=60)
                GAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATT                                                                                >  SRR4302534.2247013/1‑94 (MQ=60)
                  ATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCC                                                                              >  SRR4302534.2338794/1‑94 (MQ=60)
                   TCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCC                                                                             >  SRR4302534.2572333/1‑94 (MQ=60)
                   TCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCC                                                                             >  SRR4302534.1187638/1‑94 (MQ=60)
                    CTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCT                                                                            <  SRR4302534.4160166/94‑1 (MQ=60)
                      CTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTC                                                                          >  SRR4302534.3698115/1‑94 (MQ=60)
                       TTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCA                                                                         <  SRR4302534.543724/94‑1 (MQ=60)
                        TTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCAC                                                                        <  SRR4302534.2387039/94‑1 (MQ=60)
                           CTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTG                                                                     <  SRR4302534.1065403/94‑1 (MQ=60)
                             TGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                   <  SRR4302534.4868734/94‑1 (MQ=60)
                             GGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                   >  SRR4302534.1907291/1‑94 (MQ=60)
                             GGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                   <  SRR4302534.4796204/94‑1 (MQ=60)
                             GGTGTTGAAGGCATCCATACGAACGATGCCGACTTTATCGCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAA                                                                   <  SRR4302534.3140697/94‑1 (MQ=60)
                              GTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAG                                                                  <  SRR4302534.3605300/94‑1 (MQ=60)
                                TTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCG                                                                <  SRR4302534.55065/94‑1 (MQ=60)
                                 TTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGA                                                               <  SRR4302534.686287/94‑1 (MQ=60)
                                 TTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGA                                                               >  SRR4302534.3159222/1‑94 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAG                                                              >  SRR4302534.5675348/1‑94 (MQ=60)
                                  TGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCACTTCACCTGAAGCGAG                                                              >  SRR4302534.205773/1‑94 (MQ=60)
                                   GAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGA                                                             >  SRR4302534.5281380/1‑94 (MQ=60)
                                    AAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAT                                                            >  SRR4302534.3253775/1‑94 (MQ=60)
                                    AAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAT                                                            >  SRR4302534.4319572/1‑94 (MQ=60)
                                    AAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGAT                                                            >  SRR4302534.3974908/1‑94 (MQ=60)
                                     AGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATT                                                           >  SRR4302534.622142/1‑94 (MQ=60)
                                       GCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTA                                                         >  SRR4302534.837682/1‑94 (MQ=60)
                                        CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAA                                                        >  SRR4302534.4915927/1‑94 (MQ=60)
                                        CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAA                                                        >  SRR4302534.810047/1‑94 (MQ=60)
                                        CATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAA                                                        >  SRR4302534.4128187/1‑94 (MQ=60)
                                         ATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAAC                                                       >  SRR4302534.1676839/1‑94 (MQ=60)
                                              GACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAC                                                  >  SRR4302534.918045/1‑94 (MQ=60)
                                              GACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAC                                                  >  SRR4302534.973107/1‑94 (MQ=60)
                                              GACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCAC                                                  >  SRR4302534.1592450/1‑94 (MQ=60)
                                                CGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                                <  SRR4302534.1009879/94‑1 (MQ=60)
                                                CGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                                >  SRR4302534.1258553/1‑94 (MQ=60)
                                                CGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                                >  SRR4302534.6044734/1‑94 (MQ=60)
                                                CGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                                <  SRR4302534.2896514/94‑1 (MQ=60)
                                                CGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCT                                                <  SRR4302534.5164723/94‑1 (MQ=60)
                                                 caccgagGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                               <  SRR4302534.1431237/87‑1 (MQ=60)
                                                 GAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTT                                               >  SRR4302534.4887297/1‑94 (MQ=60)
                                                  AACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTC                                              >  SRR4302534.5928404/1‑94 (MQ=60)
                                                    CGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTG                                            >  SRR4302534.5045397/1‑94 (MQ=60)
                                                     TATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGC                                           >  SRR4302534.1719251/1‑94 (MQ=60)
                                                      ATGCGGACTTTATCTCCCGCAACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCT                                          <  SRR4302534.4548200/94‑1 (MQ=60)
                                                      ATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCT                                          <  SRR4302534.298762/94‑1 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                     >  SRR4302534.3862895/1‑94 (MQ=60)
                                                           GACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCG                                     >  SRR4302534.4644007/1‑94 (MQ=60)
                                                            ACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                    <  SRR4302534.171335/94‑1 (MQ=60)
                                                            ACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                    >  SRR4302534.5048220/1‑94 (MQ=60)
                                                            ACTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                    <  SRR4302534.5303597/94‑1 (MQ=60)
                                                            ACTTCATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGT                                    <  SRR4302534.6337431/94‑1 (MQ=60)
                                                             CTTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTT                                   <  SRR4302534.2068380/94‑1 (MQ=60)
                                                              TTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                  <  SRR4302534.4088756/94‑1 (MQ=60)
                                                              TTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                  <  SRR4302534.4447637/94‑1 (MQ=60)
                                                              TTTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTT                                  <  SRR4302534.4745178/94‑1 (MQ=60)
                                                               TTATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTT                                 <  SRR4302534.5346459/94‑1 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                                <  SRR4302534.3097738/94‑1 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                                >  SRR4302534.6598973/1‑94 (MQ=60)
                                                                TATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTC                                >  SRR4302534.4755493/1‑94 (MQ=60)
                                                                 ATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                               <  SRR4302534.5832715/94‑1 (MQ=60)
                                                                 ATCTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCG                               <  SRR4302534.1622860/94‑1 (MQ=60)
                                                                   CTCCCGCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGAT                             <  SRR4302534.3665781/94‑1 (MQ=60)
                                                                        GCGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACA                        <  SRR4302534.2266457/94‑1 (MQ=60)
                                                                         CGACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAA                       >  SRR4302534.3625880/1‑94 (MQ=60)
                                                                          GACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                      >  SRR4302534.3456703/1‑94 (MQ=60)
                                                                          GACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAA                      >  SRR4302534.1126711/1‑94 (MQ=60)
                                                                           ACATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAAC                     <  SRR4302534.1197787/94‑1 (MQ=60)
                                                                            CATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACC                    <  SRR4302534.5553048/94‑1 (MQ=60)
                                                                             ATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                   >  SRR4302534.4411288/1‑94 (MQ=60)
                                                                             ATAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCG                   <  SRR4302534.5592725/94‑1 (MQ=60)
                                                                              TAAAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGA                  <  SRR4302534.5918098/94‑1 (MQ=60)
                                                                                AAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGC                >  SRR4302534.5037966/1‑94 (MQ=60)
                                                                                AAACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGC                >  SRR4302534.2259446/1‑94 (MQ=60)
                                                                                 AACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG               <  SRR4302534.2307479/94‑1 (MQ=60)
                                                                                 AACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG               <  SRR4302534.1242467/94‑1 (MQ=60)
                                                                                 AACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCG               >  SRR4302534.3507022/1‑94 (MQ=60)
                                                                                  ACACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGG              >  SRR4302534.3002893/1‑94 (MQ=60)
                                                                                   CACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGC             >  SRR4302534.4193299/1‑94 (MQ=60)
                                                                                   CACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGC             <  SRR4302534.1737056/94‑1 (MQ=60)
                                                                                    ACGCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCG            <  SRR4302534.253985/94‑1 (MQ=60)
                                                                                      GCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCA          >  SRR4302534.3394979/1‑94 (MQ=60)
                                                                                      GCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCA          <  SRR4302534.3353996/94‑1 (MQ=60)
                                                                                      GCCGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCA          >  SRR4302534.2899052/1‑94 (MQ=60)
                                                                                        CGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA        >  SRR4302534.1776248/1‑94 (MQ=60)
                                                                                        CGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA        <  SRR4302534.5383340/94‑1 (MQ=60)
                                                                                        CGCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATA        >  SRR4302534.299596/1‑94 (MQ=60)
                                                                                         GCCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAG       <  SRR4302534.5115520/94‑1 (MQ=60)
                                                                                          CCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGC      >  SRR4302534.850791/1‑94 (MQ=60)
                                                                                          CCAGGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGC      <  SRR4302534.4809961/94‑1 (MQ=60)
                                                                                           cagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCT     <  SRR4302534.2219943/91‑1 (MQ=60)
                                                                                           cagGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCT     >  SRR4302534.1299783/4‑94 (MQ=60)
                                                                                            agGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT    >  SRR4302534.4862102/3‑94 (MQ=60)
                                                                                            agGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT    >  SRR4302534.4227689/3‑94 (MQ=60)
                                                                                            agGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT    >  SRR4302534.5398653/3‑94 (MQ=60)
                                                                                            agGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT    <  SRR4302534.459789/92‑1 (MQ=60)
                                                                                            agGACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTT    >  SRR4302534.5953901/3‑94 (MQ=60)
                                                                                              GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTC  >  SRR4302534.5352033/1‑94 (MQ=60)
                                                                                              |                                                                                             
GACTGCACCGCAAACTGAATCTCTTTACTGGTTTTGAAGGCATCCAGACGAACGATGCCGACTTTATCTCCCGCGACATAAAACACGCCGCCA‑GACGGATAAAGAAATTCCCTTCACCTGAAGCGAGATTTAACGCCACCTTCTGCTGACCGTTTTCGATGTACAAACCGAGCGGCGCATAGCTTTC  >  NC_000913/3998197‑3998383

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑