Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,004,979 +G coding (1511/1833 nt) recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,9791.G99.8% 1846.5 / ‑4.4 532coding (1511/1833 nt)recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (284/247);  total (284/248)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.37e-01

Only 100 of 589 total aligned reads displayed.

CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004887‑4005060
                                                                                             |                                                                                     
  tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgc                                                                                      <  2:5458794/93‑3 (MQ=255)
  tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgc                                                                                      >  1:1827769/1‑91 (MQ=255)
  tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgc                                                                                      <  1:4318728/93‑3 (MQ=255)
  tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgc                                                                                      >  1:3431206/1‑91 (MQ=255)
  tGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgc                                                                                      <  1:4526114/93‑3 (MQ=255)
   gATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcg                                                                                     <  2:3021543/93‑4 (MQ=255)
    aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                    <  1:1369824/93‑5 (MQ=255)
    aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                    <  2:1383600/93‑5 (MQ=255)
    aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                    <  2:7375725/93‑5 (MQ=255)
    aTCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCtgcgc                                                                                    >  2:5718492/1‑89 (MQ=255)
         ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcga                                                                             >  2:1180064/1‑90 (MQ=255)
                 ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCtt                                                                     >  2:8289535/1‑93 (MQ=255)
                 ttCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCtt                                                                     >  2:2534418/1‑93 (MQ=255)
                    cccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑GCTAATCCTCTTTGc                                                                  <  1:1653531/92‑1 (MQ=255)
                     ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCTCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCa                                                                 <  1:7060824/93‑1 (MQ=255)
                     ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCa                                                                 <  2:7949319/93‑1 (MQ=255)
                       tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAAc                                                               <  1:5603052/93‑1 (MQ=255)
                        cGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACt                                                              <  2:4491023/93‑1 (MQ=255)
                         ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACtt                                                             >  2:1104208/1‑93 (MQ=255)
                         ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACtt                                                             >  2:5158956/1‑93 (MQ=255)
                          gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtg                                                            <  1:729670/93‑1 (MQ=255)
                          gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtg                                                            >  1:1560574/1‑93 (MQ=255)
                          gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtg                                                            <  1:1443977/93‑1 (MQ=255)
                           ccTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgc                                                           <  1:3021587/93‑1 (MQ=255)
                           ccTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgc                                                           <  1:2234252/93‑1 (MQ=255)
                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  1:3334941/1‑93 (MQ=255)
                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  1:7496727/1‑93 (MQ=255)
                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  1:2570745/1‑93 (MQ=255)
                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  2:85079/1‑93 (MQ=255)
                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  2:1529053/1‑93 (MQ=255)
                              ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGAAACTtgccgt                                                        >  2:4087323/1‑93 (MQ=255)
                               gTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtg                                                       <  2:7457090/93‑1 (MQ=255)
                               gTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtg                                                       <  2:1755320/93‑1 (MQ=255)
                                 gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgcc                                                     >  2:3639594/1‑93 (MQ=255)
                                 gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgcc                                                     <  2:2699824/93‑1 (MQ=255)
                                 gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgcc                                                     >  2:2372079/1‑93 (MQ=255)
                                 gACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgcc                                                     >  1:2659269/1‑93 (MQ=255)
                                  aCGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgccg                                                    >  1:5678389/1‑93 (MQ=255)
                                   cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCggc                                                   >  1:8696630/1‑93 (MQ=255)
                                   cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCcgc                                                   <  1:8600061/93‑1 (MQ=255)
                                      aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                >  1:5442823/1‑93 (MQ=255)
                                      aaaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTa                                                <  2:7163395/93‑1 (MQ=255)
                                       aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                               <  2:1676830/93‑1 (MQ=255)
                                        aaTATTGCTCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              <  1:4958194/93‑1 (MQ=255)
                                         atatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCg                                             >  1:5855180/1‑93 (MQ=255)
                                         atatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCg                                             <  2:1899351/93‑1 (MQ=255)
                                          tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGt                                            >  1:3131750/1‑93 (MQ=255)
                                            ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          >  1:6219767/1‑93 (MQ=255)
                                             tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  2:6614977/93‑1 (MQ=255)
                                              gCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgggg                                        >  2:4244141/1‑91 (MQ=255)
                                               cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcgc                                       >  2:2190267/1‑93 (MQ=255)
                                               cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcgc                                       >  1:6469654/1‑93 (MQ=255)
                                                ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  2:4860036/1‑93 (MQ=255)
                                                ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  1:4053286/1‑93 (MQ=255)
                                                 cAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTc                                     >  1:7682385/1‑93 (MQ=255)
                                                  aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  2:6791277/1‑93 (MQ=255)
                                                   gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTTCCGTGCCGCGTATCGTGGGGCTCaa                                   >  2:8595274/1‑93 (MQ=255)
                                                   gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa                                   >  2:6067850/1‑93 (MQ=255)
                                                   gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaa                                   >  1:1203445/1‑93 (MQ=255)
                                                   gCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGACGCGTATCGTGGCGCTCaa                                   >  1:5212241/1‑93 (MQ=255)
                                                     aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 >  2:313185/1‑93 (MQ=255)
                                                      ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                >  2:6220063/1‑93 (MQ=255)
                                                          gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                            <  1:1961979/93‑1 (MQ=255)
                                                           cccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  1:6392245/93‑1 (MQ=255)
                                                           cccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  1:785851/93‑1 (MQ=255)
                                                           accTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACGTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  2:5946847/92‑1 (MQ=255)
                                                            ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          >  1:4150884/1‑93 (MQ=255)
                                                             cTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         >  2:8774501/1‑93 (MQ=255)
                                                             cTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         <  2:2862315/93‑1 (MQ=255)
                                                              tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        <  1:2428346/93‑1 (MQ=255)
                                                              tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        >  1:4849878/1‑93 (MQ=255)
                                                               aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       >  1:1061815/1‑93 (MQ=255)
                                                                 aaCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCGCAAACCGGAAGAGATGc                     >  2:3768114/1‑93 (MQ=255)
                                                                  aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    <  1:3949056/93‑1 (MQ=255)
                                                                  aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  1:5047780/1‑93 (MQ=255)
                                                                  aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  1:957223/1‑93 (MQ=255)
                                                                   cTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAg                   >  2:7619875/1‑93 (MQ=255)
                                                                      aCAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                <  2:7841230/93‑1 (MQ=255)
                                                                       cAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               >  2:2468632/1‑93 (MQ=255)
                                                                       cAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               >  2:2117000/1‑93 (MQ=255)
                                                                       cAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  1:8266905/93‑1 (MQ=255)
                                                                        agagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              <  1:6038055/93‑1 (MQ=255)
                                                                        agagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              <  2:5329117/93‑1 (MQ=255)
                                                                        agagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATc              >  2:774023/1‑93 (MQ=255)
                                                                         gagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             >  1:6827979/1‑93 (MQ=255)
                                                                          agGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt            <  2:7423489/93‑1 (MQ=255)
                                                                           ggCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  2:3542905/93‑1 (MQ=255)
                                                                           ggCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  2:7029097/93‑1 (MQ=255)
                                                                            gCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  2:3908612/93‑1 (MQ=255)
                                                                            gCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  1:8433529/93‑1 (MQ=255)
                                                                            gCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  1:6908145/93‑1 (MQ=255)
                                                                            gCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTc          <  1:6656785/93‑1 (MQ=255)
                                                                             ccGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg         <  2:5540436/93‑1 (MQ=255)
                                                                             ccGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcg         >  1:5348950/1‑93 (MQ=255)
                                                                               gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  2:4985054/1‑93 (MQ=255)
                                                                               gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  2:3300374/1‑93 (MQ=255)
                                                                               gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAACCGATGCAGAAATCGTTcggg       >  1:2018766/1‑92 (MQ=255)
                                                                                cgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      <  1:8759819/93‑1 (MQ=255)
                                                                                    cccGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa  <  1:369899/93‑1 (MQ=255)
                                                                                    cccGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCaa  >  2:5821927/1‑93 (MQ=255)
                                                                                             |                                                                                     
CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004887‑4005060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 327 total aligned reads displayed.
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004886‑4005063
                                                                                             |                                                                                         
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT                                                                                           <  SRR4302541.2220772/94‑1 (MQ=60)
 CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑G                                                                                         >  SRR4302541.3662594/1‑94 (MQ=60)
 CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑G                                                                                         >  SRR4302541.5278322/1‑94 (MQ=60)
 CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑G                                                                                         <  SRR4302541.6372410/94‑1 (MQ=60)
 CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑G                                                                                         <  SRR4302541.273940/94‑1 (MQ=60)
  GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgc                                                                                         >  SRR4302541.3465483/1‑92 (MQ=60)
  GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgc                                                                                         >  SRR4302541.8824407/1‑92 (MQ=60)
  GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgc                                                                                         <  SRR4302541.1569223/94‑3 (MQ=60)
  GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgc                                                                                         <  SRR4302541.2322056/94‑3 (MQ=60)
  GTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgc                                                                                         <  SRR4302541.8200898/94‑3 (MQ=60)
   TGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑‑GCG                                                                                      <  SRR4302541.4361846/94‑1 (MQ=60)
    GATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGC                                                                                       >  SRR4302541.3044002/1‑94 (MQ=60)
    GATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGACGCCGCGCGCCCGGTGCTGCGC                                                                                       >  SRR4302541.1155692/1‑94 (MQ=60)
     ATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACGGAGGCCGCGCGCCCGGTGCTGCGCG                                                                                      <  SRR4302541.1824706/94‑1 (MQ=60)
       CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggc                                                                                    <  SRR4302541.2618275/94‑8 (MQ=60)
       CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggc                                                                                    <  SRR4302541.1329491/94‑8 (MQ=60)
       CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggc                                                                                    <  SRR4302541.8719015/94‑8 (MQ=60)
       CCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggc                                                                                    >  SRR4302541.571513/1‑87 (MQ=60)
        CGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACACCGGACAGAGGCCGCGCGCCCGGTGCTgcgcggcg                                                                                   <  SRR4302541.6793739/94‑9 (MQ=60)
        CGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcg                                                                                   >  SRR4302541.8829473/1‑86 (MQ=60)
         GCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAAGCCGCGCGCCCGGTGCTgcgcggcga                                                                                  >  SRR4302541.8654588/1‑85 (MQ=60)
          CCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaa                                                                                 <  SRR4302541.341838/94‑11 (MQ=60)
          CCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaa                                                                                 >  SRR4302541.4510744/1‑84 (MQ=60)
           CAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaat                                                                                <  SRR4302541.4430684/94‑12 (MQ=60)
           CAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaat                                                                                >  SRR4302541.17556/1‑83 (MQ=60)
            AGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaatc                                                                               >  SRR4302541.4280024/1‑82 (MQ=60)
            AGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaatc                                                                               >  SRR4302541.6491535/1‑82 (MQ=60)
            AGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaatc                                                                               <  SRR4302541.5475171/94‑13 (MQ=60)
             GCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaatcc                                                                              >  SRR4302541.8698399/1‑81 (MQ=60)
             GCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaatcc                                                                              >  SRR4302541.3069101/1‑81 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                           >  SRR4302541.1761798/1‑94 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                           <  SRR4302541.7009988/94‑1 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                           >  SRR4302541.8372800/1‑94 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                           >  SRR4302541.515167/1‑94 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                           >  SRR4302541.183938/1‑94 (MQ=60)
               TGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTC                                                                          >  SRR4302541.2528801/1‑94 (MQ=60)
               TAATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTC                                                                          >  SRR4302541.6001072/1‑94 (MQ=60)
                  TTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTT                                                                       <  SRR4302541.7147341/94‑1 (MQ=60)
                  TTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTT                                                                       <  SRR4302541.2451288/94‑1 (MQ=60)
                   TCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTG                                                                      <  SRR4302541.1644401/94‑1 (MQ=60)
                   TCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTG                                                                      >  SRR4302541.6542119/1‑94 (MQ=60)
                    CACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGC                                                                     <  SRR4302541.5573697/94‑1 (MQ=60)
                     CCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑GCTAATCCTCTTTGCA                                                                    <  SRR4302541.1669859/94‑1 (MQ=60)
                     ACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCA                                                                    <  SRR4302541.4186922/94‑1 (MQ=60)
                     ACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCA                                                                    >  SRR4302541.6944158/1‑94 (MQ=60)
                      CCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAA                                                                   <  SRR4302541.7286072/94‑1 (MQ=60)
                      CCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAA                                                                   <  SRR4302541.6088927/94‑1 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAAC                                                                  >  SRR4302541.7969733/1‑94 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAAC                                                                  >  SRR4302541.1260647/1‑94 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAAC                                                                  >  SRR4302541.6962577/1‑94 (MQ=60)
                        TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACT                                                                 <  SRR4302541.8632427/94‑1 (MQ=60)
                        TCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACT                                                                 <  SRR4302541.5660404/94‑1 (MQ=60)
                         CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACATCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTT                                                                <  SRR4302541.8891551/94‑1 (MQ=60)
                          GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTG                                                               <  SRR4302541.8654115/94‑1 (MQ=60)
                          GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTG                                                               >  SRR4302541.2882371/1‑94 (MQ=60)
                           GCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGC                                                              <  SRR4302541.1458032/94‑1 (MQ=60)
                           GCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGC                                                              <  SRR4302541.2299417/94‑1 (MQ=60)
                            GCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                             <  SRR4302541.2538466/94‑1 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                             >  SRR4302541.4070144/1‑94 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                             <  SRR4302541.1785397/94‑1 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                             <  SRR4302541.5071001/94‑1 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                             >  SRR4302541.7576873/1‑94 (MQ=60)
                             CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCG                                                            >  SRR4302541.5351570/1‑94 (MQ=60)
                             CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCG                                                            <  SRR4302541.779086/94‑1 (MQ=60)
                             CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCG                                                            >  SRR4302541.3401440/1‑94 (MQ=60)
                              TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                           >  SRR4302541.3087843/1‑94 (MQ=60)
                              TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                           >  SRR4302541.8031629/1‑94 (MQ=60)
                               GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTG                                                          >  SRR4302541.3957382/1‑94 (MQ=60)
                               GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTG                                                          <  SRR4302541.4922385/94‑1 (MQ=60)
                               GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTG                                                          <  SRR4302541.4919331/94‑1 (MQ=60)
                                GTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGC                                                         <  SRR4302541.2793622/94‑1 (MQ=60)
                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCC                                                        >  SRR4302541.2685714/1‑94 (MQ=60)
                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCC                                                        >  SRR4302541.5035388/1‑94 (MQ=60)
                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCC                                                        >  SRR4302541.2202929/1‑94 (MQ=60)
                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGAGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTCGCAACTTGCCGTGCC                                                        >  SRR4302541.6503892/1‑94 (MQ=60)
                                  GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCG                                                       >  SRR4302541.5736329/1‑94 (MQ=60)
                                  GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCG                                                       <  SRR4302541.2007208/94‑1 (MQ=60)
                                  GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCG                                                       <  SRR4302541.4079410/94‑1 (MQ=60)
                                   ACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGC                                                      <  SRR4302541.1940551/94‑1 (MQ=60)
                                      CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                   >  SRR4302541.5498672/1‑94 (MQ=60)
                                      CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                   >  SRR4302541.3492694/1‑94 (MQ=60)
                                       AAAATATTGCCCAGCATTCTGCCCTACAGCTGACGGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTAT                                                  <  SRR4302541.4826427/94‑1 (MQ=60)
                                        AAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATC                                                 <  SRR4302541.2576309/94‑1 (MQ=60)
                                        AAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATC                                                 <  SRR4302541.1557654/94‑1 (MQ=60)
                                         AATATTGCTCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCG                                                <  SRR4302541.5008934/94‑1 (MQ=60)
                                         AATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCG                                                >  SRR4302541.5914719/1‑94 (MQ=60)
                                          ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                               >  SRR4302541.8790406/1‑94 (MQ=60)
                                          ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                               >  SRR4302541.5464208/1‑94 (MQ=60)
                                          ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                               >  SRR4302541.3163400/1‑94 (MQ=60)
                                          ATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGT                                               >  SRR4302541.265957/1‑94 (MQ=60)
                                           TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                              >  SRR4302541.736628/1‑94 (MQ=60)
                                           TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                              <  SRR4302541.2455075/94‑1 (MQ=60)
                                            ATTGGCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                             <  SRR4302541.6650587/94‑1 (MQ=60)
                                            ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                             >  SRR4302541.7123264/1‑94 (MQ=60)
                                            ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                             >  SRR4302541.3105309/1‑94 (MQ=60)
                                            ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                             <  SRR4302541.542880/94‑1 (MQ=60)
                                             TTGCCCAGCAGTCTGCGCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGC                                            <  SRR4302541.2560759/94‑1 (MQ=60)
                                              TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           <  SRR4302541.6222869/94‑1 (MQ=60)
                                              TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           <  SRR4302541.5518901/94‑1 (MQ=60)
                                              TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           >  SRR4302541.3222827/1‑94 (MQ=60)
                                               GCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGC                                          >  SRR4302541.6535590/1‑94 (MQ=60)
                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         >  SRR4302541.705182/1‑94 (MQ=60)
                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         >  SRR4302541.4093283/1‑94 (MQ=60)
                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         >  SRR4302541.7408779/1‑94 (MQ=60)
                                                 CCAGCATTCTGCCCTACAACTGACATAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCCACTTGCCCTGCCGCGTATCATGGCGCTC                                        >  SRR4302541.4077537/1‑94 (MQ=60)
                                                 CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                        >  SRR4302541.8507436/1‑94 (MQ=60)
                                                 CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                        <  SRR4302541.1849862/94‑1 (MQ=60)
                                                 CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                        >  SRR4302541.7764053/1‑94 (MQ=60)
                                                 CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                        >  SRR4302541.1923084/1‑94 (MQ=60)
                                                  CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                       >  SRR4302541.5583151/1‑94 (MQ=60)
                                                   AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      >  SRR4302541.5705781/1‑94 (MQ=60)
                                                   AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      >  SRR4302541.5265945/1‑94 (MQ=60)
                                                   AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      >  SRR4302541.1215132/1‑94 (MQ=60)
                                                   AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      <  SRR4302541.4246643/94‑1 (MQ=60)
                                                   AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      <  SRR4302541.8215228/94‑1 (MQ=60)
                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    >  SRR4302541.48963/1‑94 (MQ=60)
                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   <  SRR4302541.4256899/94‑1 (MQ=60)
                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   >  SRR4302541.3333742/1‑94 (MQ=60)
                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   >  SRR4302541.6467053/1‑94 (MQ=60)
                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   <  SRR4302541.1516503/94‑1 (MQ=60)
                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   >  SRR4302541.5958398/1‑94 (MQ=60)
                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   <  SRR4302541.3288026/94‑1 (MQ=60)
                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   >  SRR4302541.888728/1‑94 (MQ=60)
                                                       TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCG                                  <  SRR4302541.7489715/94‑1 (MQ=60)
                                                       TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCG                                  >  SRR4302541.4631353/1‑94 (MQ=60)
                                                         CTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGCGCCGCGTATCGTGGCGCTCAAACCGAA                                >  SRR4302541.7709997/1‑94 (MQ=60)
                                                          TGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAA                               >  SRR4302541.8780811/1‑94 (MQ=60)
                                                          TGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAA                               <  SRR4302541.1446095/94‑1 (MQ=60)
                                                          TGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAA                               <  SRR4302541.1446094/94‑1 (MQ=60)
                                                           GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                              <  SRR4302541.584765/94‑1 (MQ=60)
                                                           GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                              <  SRR4302541.1259223/94‑1 (MQ=60)
                                                           GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                              >  SRR4302541.733238/1‑94 (MQ=60)
                                                           GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                              <  SRR4302541.1980895/94‑1 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             <  SRR4302541.6457498/94‑1 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             >  SRR4302541.3390255/1‑94 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             <  SRR4302541.793148/94‑1 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             >  SRR4302541.6790300/1‑94 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             <  SRR4302541.736664/94‑1 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             >  SRR4302541.4191926/1‑94 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             <  SRR4302541.3637843/94‑1 (MQ=60)
                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            >  SRR4302541.6392370/1‑94 (MQ=60)
                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            >  SRR4302541.6392372/1‑94 (MQ=60)
                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            >  SRR4302541.5101981/1‑94 (MQ=60)
                                                              CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                           >  SRR4302541.1397138/1‑94 (MQ=60)
                                                              CTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGA                           >  SRR4302541.4899327/1‑94 (MQ=60)
                                                               TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCGTGTTTGCAAATTGCAATGCCGCGTATCGTGGCGCTCAACCCGAAAGCGAG                          >  SRR4302541.5041830/1‑94 (MQ=44)
                                                               TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          >  SRR4302541.1071679/1‑94 (MQ=60)
                                                               TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          >  SRR4302541.3514410/1‑94 (MQ=60)
                                                               TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          >  SRR4302541.4576034/1‑94 (MQ=60)
                                                                ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                         >  SRR4302541.2623305/1‑94 (MQ=60)
                                                                 CAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGTCGCTCAAACCGAAAGCGATGC                        <  SRR4302541.3071937/94‑1 (MQ=60)
                                                                 CAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGC                        >  SRR4302541.8789159/1‑94 (MQ=60)
                                                                 CAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGC                        <  SRR4302541.688002/94‑1 (MQ=60)
                                                                 CAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGC                        >  SRR4302541.7779438/1‑94 (MQ=60)
                                                                 CAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGC                        <  SRR4302541.4142767/94‑1 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       >  SRR4302541.4387799/1‑94 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       <  SRR4302541.7019726/94‑1 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       >  SRR4302541.5099721/1‑94 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       >  SRR4302541.1750012/1‑94 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       >  SRR4302541.6900857/1‑94 (MQ=60)
                                                                   ACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAACGCGAAAGCGATGCAA                      >  SRR4302541.7649269/1‑94 (MQ=60)
                                                                   ACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                      <  SRR4302541.2171567/94‑1 (MQ=60)
                                                                      GACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAA                   >  SRR4302541.8760426/1‑94 (MQ=60)
                                                                       ACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAT                  <  SRR4302541.5229726/94‑1 (MQ=60)
                                                                        CAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 <  SRR4302541.7660660/94‑1 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCCAACCGAAAGCGATGCAGAAATAG                >  SRR4302541.1579379/1‑94 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                >  SRR4302541.2002565/1‑94 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                >  SRR4302541.6901040/1‑94 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                <  SRR4302541.6099868/94‑1 (MQ=60)
                                                                           AGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              <  SRR4302541.964396/94‑1 (MQ=60)
                                                                           AGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              <  SRR4302541.8466457/94‑1 (MQ=60)
                                                                           AGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              >  SRR4302541.3867362/1‑94 (MQ=60)
                                                                            GGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             <  SRR4302541.2022022/94‑1 (MQ=60)
                                                                            GGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             >  SRR4302541.3885932/1‑94 (MQ=60)
                                                                            GGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             >  SRR4302541.5776239/1‑94 (MQ=60)
                                                                            GGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             <  SRR4302541.623309/94‑1 (MQ=60)
                                                                             GCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            <  SRR4302541.6981867/94‑1 (MQ=60)
                                                                             GCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            >  SRR4302541.5403925/1‑94 (MQ=60)
                                                                             GCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            <  SRR4302541.6351193/94‑1 (MQ=60)
                                                                             GCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            <  SRR4302541.6918300/94‑1 (MQ=60)
                                                                             GCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            <  SRR4302541.6471247/94‑1 (MQ=60)
                                                                             GCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCG            <  SRR4302541.6725077/94‑1 (MQ=60)
                                                                              CCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG           <  SRR4302541.1578581/94‑1 (MQ=60)
                                                                              CCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG           >  SRR4302541.8435543/1‑94 (MQ=60)
                                                                               CGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAACCGATGCAGAAATCGTTCGGG          >  SRR4302541.2038296/1‑94 (MQ=60)
                                                                                GCGCGCGCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         <  SRR4302541.5221738/94‑1 (MQ=60)
                                                                                GCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         <  SRR4302541.6011808/94‑1 (MQ=60)
                                                                                GCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         >  SRR4302541.1138176/1‑94 (MQ=60)
                                                                                GCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         >  SRR4302541.618336/1‑94 (MQ=60)
                                                                                 CGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGG        <  SRR4302541.8853514/94‑1 (MQ=60)
                                                                                  GCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCCAACCGAAAGCGATGCAGAAATCGTTCGGCGGC       >  SRR4302541.2107571/1‑94 (MQ=60)
                                                                                  GCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGC       <  SRR4302541.4305911/94‑1 (MQ=60)
                                                                                    GCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA     <  SRR4302541.3968602/94‑1 (MQ=60)
                                                                                    GCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA     <  SRR4302541.5684373/94‑1 (MQ=60)
                                                                                    GCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA     >  SRR4302541.725471/1‑94 (MQ=60)
                                                                                     CCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC    <  SRR4302541.373274/94‑1 (MQ=60)
                                                                                     CCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAAC    >  SRR4302541.5865938/1‑94 (MQ=60)
                                                                                      CCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   >  SRR4302541.8524005/1‑94 (MQ=60)
                                                                                       CGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  SRR4302541.1424046/1‑94 (MQ=60)
                                                                                       CGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  <  SRR4302541.1091311/94‑1 (MQ=60)
                                                                                             |                                                                                         
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004886‑4005063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑