Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,004,979 +G coding (1511/1833 nt) recQ → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,004,9791.G100.0% 837.0 / NA 238L504R (CTC→CGC) recQATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base . (0/0);  new base G (133/105);  total (133/105)

Only 100 of 248 total aligned reads displayed.

CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004887‑4005060
                                                                                             |                                                                                     
cGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCt                                                                                        >  1:3488862/1‑93 (MQ=255)
        gCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGACGCGCGCCCGGTGCTGCGCg‑‑gcg                                                                              >  1:2076544/1‑91 (MQ=255)
         ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcga                                                                             >  2:2818053/1‑90 (MQ=255)
         ccAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcga                                                                             >  2:2635384/1‑90 (MQ=255)
          cAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcgaa                                                                            >  1:1120973/1‑89 (MQ=255)
          cAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCg‑‑gcgaa                                                                            <  1:3125079/93‑5 (MQ=255)
                aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCctct                                                                      >  2:2990603/1‑93 (MQ=255)
                aTTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCctct                                                                      <  2:334812/93‑1 (MQ=255)
                   cACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGACGTCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTg                                                                   >  1:1525301/1‑93 (MQ=255)
                    aCCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGc                                                                  >  2:1006707/1‑93 (MQ=255)
                     ccTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCa                                                                 <  1:3286022/93‑1 (MQ=255)
                       tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAAc                                                               >  1:606636/1‑93 (MQ=255)
                       tCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAAc                                                               >  1:3605715/1‑93 (MQ=255)
                         ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACtt                                                             >  1:5651041/1‑93 (MQ=255)
                         ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACtt                                                             <  1:1503351/93‑1 (MQ=255)
                         ggCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACtt                                                             <  2:3224096/93‑1 (MQ=255)
                          gCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtg                                                            >  2:4641085/1‑93 (MQ=255)
                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  2:2007408/1‑93 (MQ=255)
                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  1:2205026/1‑93 (MQ=255)
                            cTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgcc                                                          >  2:153339/1‑93 (MQ=255)
                             tGGTGACGCAAAATATTGCCCATCATTCTGCCCTACAACAGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccg                                                         <  1:4169618/93‑1 (MQ=255)
                             tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccg                                                         <  2:588515/93‑1 (MQ=255)
                             tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccg                                                         <  1:6116615/93‑1 (MQ=255)
                             tGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccg                                                         >  2:193799/1‑93 (MQ=255)
                              ggTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgt                                                        >  1:1794270/1‑93 (MQ=255)
                                tGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgc                                                      <  1:5711052/93‑1 (MQ=255)
                                tGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgc                                                      <  2:3050715/93‑1 (MQ=255)
                                  aCGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTtgccgtgccg                                                    >  1:4502750/1‑93 (MQ=255)
                                   cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCcgc                                                   <  2:5517915/93‑1 (MQ=255)
                                   cGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCcgc                                                   <  1:445969/93‑1 (MQ=255)
                                    gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCcgcg                                                  >  1:3449666/1‑93 (MQ=255)
                                    gCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCcgcg                                                  <  1:4057141/93‑1 (MQ=255)
                                       aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                               <  2:2303974/93‑1 (MQ=255)
                                       aaaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTAt                                               <  2:388971/93‑1 (MQ=255)
                                        aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              >  2:4036507/1‑93 (MQ=255)
                                        aaTATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATc                                              <  1:5478600/93‑1 (MQ=255)
                                          tatTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGt                                            <  1:1706283/93‑1 (MQ=255)
                                            ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          >  2:1453881/1‑93 (MQ=255)
                                            ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          <  2:4849035/93‑1 (MQ=255)
                                            ttGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTgg                                          >  2:2775109/1‑93 (MQ=255)
                                             tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  2:5154474/93‑1 (MQ=255)
                                             tGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgc                                         <  1:3727594/93‑1 (MQ=255)
                                              gCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcg                                        >  1:5367498/1‑93 (MQ=255)
                                              gCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcg                                        >  1:5097392/1‑93 (MQ=255)
                                               cccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGgcgc                                       <  1:3925367/93‑1 (MQ=255)
                                                ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  1:2583600/1‑93 (MQ=255)
                                                ccAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCt                                      >  2:4098679/1‑93 (MQ=255)
                                                 cAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTc                                     >  1:1013467/1‑93 (MQ=255)
                                                  cgCATTCTCCCCCAGAACTGACAGAGGCCGCGCCCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    <  2:1612107/92‑1 (MQ=255)
                                                  aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  2:6018738/1‑93 (MQ=255)
                                                  aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    >  1:685176/1‑93 (MQ=255)
                                                  aGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCa                                    <  1:1246157/93‑1 (MQ=255)
                                                    cATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCaaa                                  >  1:953716/1‑93 (MQ=255)
                                                     aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 >  2:4900491/1‑93 (MQ=255)
                                                     aTTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAc                                 <  2:4357734/93‑1 (MQ=255)
                                                      ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                >  2:1160143/1‑93 (MQ=255)
                                                      ttCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAcc                                <  1:2030923/93‑1 (MQ=255)
                                                       tCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCg                               <  2:775790/93‑1 (MQ=255)
                                                       tCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCg                               <  1:5357482/93‑1 (MQ=255)
                                                         tGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             >  1:4642199/1‑93 (MQ=255)
                                                         tGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             >  2:3259203/1‑93 (MQ=255)
                                                         tGCCCTACAACTGACAGAGGCAGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaa                             >  1:5128312/1‑93 (MQ=255)
                                                          gCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGaaa                            <  1:4986242/93‑1 (MQ=255)
                                                           cccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  1:4147639/93‑1 (MQ=255)
                                                           cccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAg                           <  2:640998/93‑1 (MQ=255)
                                                            ccTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGc                          <  2:2905697/93‑1 (MQ=255)
                                                             cTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCg                         <  2:5475407/93‑1 (MQ=255)
                                                              tACAGCTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑GCAAATCCTCTTTGCAGCTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        <  1:3019840/93‑1 (MQ=255)
                                                              tACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGa                        <  2:3732020/93‑1 (MQ=255)
                                                               aCAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAt                       <  1:5197562/93‑1 (MQ=255)
                                                                 aaCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGc                     >  2:4211958/1‑93 (MQ=255)
                                                                  aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  2:6428107/1‑93 (MQ=255)
                                                                  aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  2:2049498/1‑93 (MQ=255)
                                                                  aCTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCa                    >  2:4486804/1‑93 (MQ=255)
                                                                   cTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAg                   >  2:5989779/1‑93 (MQ=255)
                                                                     gACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaa                 >  2:501401/1‑93 (MQ=255)
                                                                      aCAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                >  2:860837/1‑93 (MQ=255)
                                                                      aCAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                <  2:3377268/93‑1 (MQ=255)
                                                                      aCAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGaaa                >  1:292092/1‑93 (MQ=255)
                                                                       cAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAt               <  2:4994591/93‑1 (MQ=255)
                                                                         gagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             >  2:2546406/1‑93 (MQ=255)
                                                                         gagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             >  2:1637728/1‑93 (MQ=255)
                                                                         gagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             <  1:4311992/93‑1 (MQ=255)
                                                                         gagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             >  2:5828275/1‑93 (MQ=255)
                                                                         gagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCg             <  2:5714048/93‑1 (MQ=255)
                                                                         gagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAGCg             >  1:2272424/1‑93 (MQ=255)
                                                                         gagGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGAGATGCAGAAATCg             >  1:2410903/1‑93 (MQ=255)
                                                                          agGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt            >  1:6335687/1‑93 (MQ=255)
                                                                          agGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGt            >  2:1703361/1‑93 (MQ=255)
                                                                           ggCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           >  2:1860798/1‑93 (MQ=255)
                                                                           ggCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGtt           <  2:1285284/93‑1 (MQ=255)
                                                                               gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  2:3655302/1‑93 (MQ=255)
                                                                               gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  1:5850273/1‑93 (MQ=255)
                                                                               gcgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggc       >  2:5294461/1‑93 (MQ=255)
                                                                                cgcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcg      <  2:2200410/93‑1 (MQ=255)
                                                                                 gcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcgg     >  2:6282554/1‑93 (MQ=255)
                                                                                 gcgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTcggcgg     <  2:3060163/93‑1 (MQ=255)
                                                                                  cgcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCggcggc    >  2:6171205/1‑93 (MQ=255)
                                                                                   gcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCa   >  2:557269/1‑93 (MQ=255)
                                                                                   gcCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCa   >  1:4668474/1‑93 (MQ=255)
                                                                                             |                                                                                     
CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA  >  NC_000913/4004887‑4005060

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004886‑4005063
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GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT                                                                                           >  SRR4302534.3582632/1‑94 (MQ=60)
 CGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑G                                                                                         <  SRR4302534.6380739/94‑1 (MQ=60)
     ATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG                                                                                      >  SRR4302534.3501314/1‑94 (MQ=60)
      TCCGCCAGCTGATTCACCTCGGCCTTGCGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTCCTGCGCG‑‑G                                                                                   >  SRR4302534.5465594/1‑94 (MQ=60)
        CGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcg                                                                                   <  SRR4302534.5983756/94‑9 (MQ=60)
        CGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGACGCGCGCCCGGTGCTgcgcggcg                                                                                   >  SRR4302534.2130913/1‑86 (MQ=60)
          CCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaa                                                                                 >  SRR4302534.1150266/1‑84 (MQ=60)
          CCAGCTGATCCACCTCGGCGTGGTGACGAAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCGGGTGCTgcgcggcgaa                                                                                 <  SRR4302534.3705459/94‑11 (MQ=60)
           CAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaat                                                                                <  SRR4302534.3207233/94‑12 (MQ=60)
            AGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaatc                                                                               >  SRR4302534.2747812/1‑82 (MQ=60)
            AGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaatc                                                                               <  SRR4302534.1954775/94‑13 (MQ=60)
             GCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTgcgcggcgaatcc                                                                              >  SRR4302534.3075698/1‑81 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                           <  SRR4302534.4330616/94‑1 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                           >  SRR4302534.2673694/1‑94 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                           >  SRR4302534.3344603/1‑94 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                           <  SRR4302534.3506542/94‑1 (MQ=60)
              CTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCT                                                                           <  SRR4302534.1210310/94‑1 (MQ=60)
                GATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGCCAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCT                                                                         <  SRR4302534.1844409/94‑1 (MQ=60)
                GATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCT                                                                         >  SRR4302534.1911995/1‑94 (MQ=60)
                 ATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTT                                                                        >  SRR4302534.1254167/1‑94 (MQ=60)
                  TTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTT                                                                       >  SRR4302534.3500190/1‑94 (MQ=60)
                  TTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTT                                                                       >  SRR4302534.2710862/1‑94 (MQ=60)
                   TCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTG                                                                      >  SRR4302534.1374656/1‑94 (MQ=60)
                   TCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGACGTCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTG                                                                      >  SRR4302534.1565347/1‑94 (MQ=60)
                    CACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGC                                                                     <  SRR4302534.2117535/94‑1 (MQ=60)
                     ACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCA                                                                    >  SRR4302534.78598/1‑94 (MQ=60)
                      CCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAA                                                                   <  SRR4302534.3373630/94‑1 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAAC                                                                  >  SRR4302534.3702674/1‑94 (MQ=60)
                       CTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAAC                                                                  >  SRR4302534.621398/1‑94 (MQ=60)
                         GGGCCTGGTGACGCAAAATATTGCGCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTT                                                                <  SRR4302534.3570628/94‑1 (MQ=60)
                         CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTT                                                                >  SRR4302534.5804001/1‑94 (MQ=60)
                         CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTT                                                                >  SRR4302534.4179522/1‑94 (MQ=60)
                         CGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTT                                                                >  SRR4302534.1392796/1‑94 (MQ=60)
                          GGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTG                                                               <  SRR4302534.1542906/94‑1 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                             >  SRR4302534.523158/1‑94 (MQ=60)
                            CCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCC                                                             >  SRR4302534.2263381/1‑94 (MQ=60)
                             CTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCG                                                            <  SRR4302534.5848413/94‑1 (MQ=60)
                              TGGTGACGCAAAATATTGCCCATCATTCTGCCCTACAACAGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                           <  SRR4302534.4282249/94‑1 (MQ=60)
                              TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                           <  SRR4302534.6283004/94‑1 (MQ=60)
                              TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                           >  SRR4302534.1840872/1‑94 (MQ=60)
                              TGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGT                                                           >  SRR4302534.2260210/1‑94 (MQ=60)
                               GGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTG                                                          <  SRR4302534.3625645/94‑1 (MQ=60)
                               GGTGACGCAAAATATGGCCCATCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTG                                                          <  SRR4302534.3240273/94‑1 (MQ=60)
                                GTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGC                                                         <  SRR4302534.2254923/94‑1 (MQ=60)
                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCC                                                        <  SRR4302534.3181587/94‑1 (MQ=60)
                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCC                                                        <  SRR4302534.5865769/94‑1 (MQ=60)
                                 TGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCC                                                        <  SRR4302534.6260479/94‑1 (MQ=60)
                                  GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCG                                                       >  SRR4302534.4624621/1‑94 (MQ=60)
                                  GACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCG                                                       <  SRR4302534.1127131/94‑1 (MQ=60)
                                   ACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGC                                                      >  SRR4302534.2271185/1‑94 (MQ=60)
                                   ACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGC                                                      >  SRR4302534.751358/1‑94 (MQ=60)
                                    CGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCG                                                     <  SRR4302534.457280/94‑1 (MQ=60)
                                    CGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCG                                                     <  SRR4302534.978933/94‑1 (MQ=60)
                                    CGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCG                                                     <  SRR4302534.4669184/94‑1 (MQ=60)
                                    CGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCG                                                     >  SRR4302534.3542391/1‑94 (MQ=60)
                                     GCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGT                                                    <  SRR4302534.4165967/94‑1 (MQ=60)
                                      CAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTA                                                   <  SRR4302534.3938597/94‑1 (MQ=60)
                                         AATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCG                                                <  SRR4302534.5626333/94‑1 (MQ=60)
                                           TATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTG                                              <  SRR4302534.1750612/94‑1 (MQ=60)
                                            ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                             <  SRR4302534.2499838/94‑1 (MQ=60)
                                            ATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGG                                             <  SRR4302534.5815026/94‑1 (MQ=60)
                                              TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           >  SRR4302534.5234969/1‑94 (MQ=60)
                                              TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           >  SRR4302534.5512156/1‑94 (MQ=60)
                                              TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           <  SRR4302534.3827775/94‑1 (MQ=60)
                                              TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           >  SRR4302534.1588860/1‑94 (MQ=60)
                                              TGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCG                                           <  SRR4302534.4237670/94‑1 (MQ=60)
                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         >  SRR4302534.4425324/1‑94 (MQ=60)
                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         >  SRR4302534.2651996/1‑94 (MQ=60)
                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         >  SRR4302534.3194038/1‑94 (MQ=60)
                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         >  SRR4302534.3997892/1‑94 (MQ=60)
                                                CCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCT                                         <  SRR4302534.4030523/94‑1 (MQ=60)
                                                 CCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTC                                        >  SRR4302534.1039547/1‑94 (MQ=60)
                                                  CAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCA                                       >  SRR4302534.701912/1‑94 (MQ=60)
                                                   AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      <  SRR4302534.1279001/94‑1 (MQ=60)
                                                   AGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAA                                      >  SRR4302534.6103205/1‑94 (MQ=60)
                                                    GCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAA                                     >  SRR4302534.978000/1‑94 (MQ=60)
                                                    GCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAA                                     <  SRR4302534.4268062/94‑1 (MQ=60)
                                                     CATTCTGCCCTACAACTGACAGAGGGCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    >  SRR4302534.6395810/1‑94 (MQ=60)
                                                     CATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAAC                                    <  SRR4302534.6640702/94‑1 (MQ=60)
                                                      ATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACC                                   <  SRR4302534.5743235/94‑1 (MQ=60)
                                                       TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCG                                  <  SRR4302534.2084036/94‑1 (MQ=60)
                                                       TTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCG                                  <  SRR4302534.559327/94‑1 (MQ=60)
                                                        TCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGA                                 <  SRR4302534.5501901/94‑1 (MQ=60)
                                                         CTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAA                                >  SRR4302534.4768195/1‑94 (MQ=60)
                                                         CTGCCCTACAACTGACAGAGGCAGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAA                                >  SRR4302534.5266767/1‑94 (MQ=60)
                                                           GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                              <  SRR4302534.5121173/94‑1 (MQ=60)
                                                           GCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑GCTAATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAG                              <  SRR4302534.1963570/94‑1 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             <  SRR4302534.4259596/94‑1 (MQ=60)
                                                            CCCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGC                             >  SRR4302534.3821755/1‑94 (MQ=60)
                                                             CCTACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCG                            >  SRR4302534.2360798/1‑94 (MQ=60)
                                                               TACAGCTGACAGAGGCCGCGCGCCCGGTGCTGCGCG‑‑GCAAATCCTCTTTGCAGCTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          <  SRR4302534.3098910/94‑1 (MQ=60)
                                                               TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          <  SRR4302534.2362069/94‑1 (MQ=60)
                                                               TACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGAT                          >  SRR4302534.5242766/1‑94 (MQ=60)
                                                                TCAACTGACAAAGGCCTCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                         <  SRR4302534.341198/94‑1 (MQ=60)
                                                                ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                         <  SRR4302534.5337737/94‑1 (MQ=60)
                                                                ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                         <  SRR4302534.4406359/94‑1 (MQ=60)
                                                                ACAACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATG                         <  SRR4302534.5584220/94‑1 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       >  SRR4302534.1543915/1‑94 (MQ=60)
                                                                  AACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCA                       <  SRR4302534.3684699/94‑1 (MQ=60)
                                                                   ACTGACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAG                      <  SRR4302534.4583889/94‑1 (MQ=60)
                                                                      GACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAA                   >  SRR4302534.299646/1‑94 (MQ=60)
                                                                       ACAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAT                  >  SRR4302534.5149741/1‑94 (MQ=60)
                                                                        CAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 <  SRR4302534.4885373/94‑1 (MQ=60)
                                                                        CAGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATC                 >  SRR4302534.4113195/1‑94 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                <  SRR4302534.508239/94‑1 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCG                >  SRR4302534.1586253/1‑94 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAAGCG                >  SRR4302534.2332684/1‑94 (MQ=60)
                                                                         AGAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGAGATGCAGAAATCG                >  SRR4302534.2475015/1‑94 (MQ=60)
                                                                          GAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGT               >  SRR4302534.6506839/1‑94 (MQ=60)
                                                                          GAGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGT               <  SRR4302534.4428809/94‑1 (MQ=60)
                                                                           AGGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTT              <  SRR4302534.2749878/94‑1 (MQ=60)
                                                                            GGCCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTC             <  SRR4302534.5317698/94‑1 (MQ=60)
                                                                              CCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG           >  SRR4302534.549472/1‑94 (MQ=60)
                                                                              CCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG           <  SRR4302534.3060177/94‑1 (MQ=60)
                                                                              CCGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGG           >  SRR4302534.4772285/1‑94 (MQ=60)
                                                                               CGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC          >  SRR4302534.6009128/1‑94 (MQ=60)
                                                                               CGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC          >  SRR4302534.1774212/1‑94 (MQ=60)
                                                                               CGCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGC          >  SRR4302534.6036308/1‑94 (MQ=60)
                                                                                GCGCGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCG         >  SRR4302534.3407002/1‑94 (MQ=60)
                                                                                   CGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGTTGCAGAAATCGTTCGGCGGCA      <  SRR4302534.5393899/94‑1 (MQ=60)
                                                                                   CGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCA      >  SRR4302534.4646921/1‑94 (MQ=60)
                                                                                   CGCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCA      >  SRR4302534.4795210/1‑94 (MQ=60)
                                                                                    GCCCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAA     >  SRR4302534.4519470/1‑94 (MQ=60)
                                                                                      CCGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACT   >  SRR4302534.621843/1‑94 (MQ=60)
                                                                                       CGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  <  SRR4302534.2879173/94‑1 (MQ=60)
                                                                                       CGGTGCTGCGCGGCG‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  <  SRR4302534.4631828/94‑1 (MQ=60)
                                                                                             |                                                                                         
GCGTGATCCGCCAGCTGATTCACCTCGGCCTGGTGACGCAAAATATTGCCCAGCATTCTGCCCTACAACTGACAGAGGCCGCGCGCCCGGTGCT‑CGCG‑‑G‑‑AATCCTCTTTGCAACTTGCCGTGCCGCGTATCGTGGCGCTCAAACCGAAAGCGATGCAGAAATCGTTCGGCGGCAACTA  >  NC_000913/4004886‑4005063

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 2 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑