Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 744 338.8 17650688 97.8% 17262372 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,525 +G intergenic (+92/‑141) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,5241.G99.5% 1953.3 / 0.1 556intergenic (+91/‑142)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/3);  new base G (262/291);  total (262/294)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

Only 100 of 599 total aligned reads displayed.

CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT  >  NC_000913/4008434‑4008611
                                                                                              |                                                                                        
cGGGATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCt                                                                                           <  1:2309558/93‑1 (MQ=255)
 gggATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g                                                                                         >  2:4298491/1‑93 (MQ=255)
 gggATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g                                                                                         >  1:3102201/1‑93 (MQ=255)
  ggATGAACGACGCCGAAATGCTGTCGATGGCC‑GAGAAAGGCGGCAGTATCGGCAGTGCGCACCAGCGCCTGAAAGACCGCCATCCCGAGCT‑gg                                                                                        >  1:1013688/1‑92 (MQ=255)
  ggATGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                                                        >  1:2604097/1‑92 (MQ=255)
    aTGAACGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑ggaa                                                                                      >  1:185502/1‑90 (MQ=255)
       aaCGACGCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTg                                                                                   <  1:6873896/93‑1 (MQ=255)
        acgacgCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGa                                                                                  >  1:3995786/1‑93 (MQ=255)
          gacgCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGAtt                                                                                <  1:8483931/93‑1 (MQ=255)
           acgCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTg                                                                               <  1:3666385/93‑1 (MQ=255)
           acgCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTg                                                                               >  2:3270963/1‑93 (MQ=255)
           acgCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GGGATTg                                                                               >  1:802885/1‑93 (MQ=255)
            cgCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTgg                                                                              <  1:27685/93‑1 (MQ=255)
            cgCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTgg                                                                              <  1:7480367/93‑1 (MQ=255)
             gCCGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGt                                                                             >  1:2435992/1‑93 (MQ=255)
              ccGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTa                                                                            >  2:7261322/1‑93 (MQ=255)
              ccGAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTa                                                                            >  2:8084251/1‑93 (MQ=255)
                gAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACt                                                                          >  2:6622730/1‑93 (MQ=255)
                gAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACt                                                                          >  1:2507149/1‑93 (MQ=255)
                gAAATGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACt                                                                          >  1:4817653/1‑93 (MQ=255)
                  aaTGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTaa                                                                        >  1:4668917/1‑93 (MQ=255)
                   aTGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAAt                                                                       <  2:6388909/93‑1 (MQ=255)
                   aTGCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAAt                                                                       <  1:3743500/93‑1 (MQ=255)
                     gCTGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGc                                                                     <  1:4746474/93‑1 (MQ=255)
                     gCTGTCGATGG‑CGGGGAAAGGCTGCATTAGGGGCAGTGCGCACCAGCGTCTGAAAGCCCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGc                                                                     <  1:5760016/93‑1 (MQ=255)
                      gtGTCGAGGG‑CGGGGAAATGCTGCATTAGGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGcc                                                                    <  1:1337000/92‑1 (MQ=255)
                       tGTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCcg                                                                   >  2:2429316/1‑93 (MQ=255)
                        gTCGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCcga                                                                  >  1:7261358/1‑93 (MQ=255)
                          cGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCcgacg                                                                <  2:5083270/93‑1 (MQ=255)
                          cGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCcgacg                                                                >  1:8265691/1‑93 (MQ=255)
                          cGATGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCcgacg                                                                <  2:7621468/93‑1 (MQ=255)
                           gATGG‑CGGGGAAAGGCTGCATTATGGGCATTGCGCTCCAGGGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCggacga                                                               >  1:4910888/1‑93 (MQ=255)
                            aTGG‑CGGGGAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCgacgac                                                              >  2:4789220/1‑93 (MQ=255)
                               ggcggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTCATGCCGACGAcgc                                                            >  1:7957934/5‑93 (MQ=255)
                                 cggggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCgg                                                          >  2:2036969/3‑93 (MQ=255)
                                   gggAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGCTTGGTACTAATGCCGACGACGCGGTg                                                        >  1:345811/1‑93 (MQ=255)
                                      aaaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCg                                                     >  1:3344735/5‑93 (MQ=255)
                                      aaaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCg                                                     >  2:6089512/5‑93 (MQ=255)
                                      aaaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCg                                                     >  2:3463479/5‑93 (MQ=255)
                                       aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGc                                                    >  2:7000991/4‑93 (MQ=255)
                                         ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                                                  >  1:1645545/2‑93 (MQ=255)
                                         ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                                                  >  2:3720534/2‑93 (MQ=255)
                                         ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                                                  >  1:5205679/2‑93 (MQ=255)
                                           cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa                                                <  2:6552626/93‑1 (MQ=255)
                                           cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa                                                <  2:5769911/93‑1 (MQ=255)
                                           cTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTa                                                <  1:430218/93‑1 (MQ=255)
                                            tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt                                               >  2:626677/1‑93 (MQ=255)
                                             gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                              <  1:5966941/93‑1 (MQ=255)
                                              cATTGTGGGCAGTGCGCGCCGGCGTCTGACCGCCCTTCATCCCGAGCT‑GAAGGTGATTGGTACTAGTGCCGACGCCGCGGTGCCGCATTTTCt                                             <  1:6707415/93‑1 (MQ=255)
                                               aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg                                            >  2:185248/1‑93 (MQ=255)
                                               aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGAATGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg                                            <  2:5223263/93‑1 (MQ=255)
                                                ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc                                           <  1:6999595/93‑1 (MQ=255)
                                                ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc                                           >  2:1728973/1‑93 (MQ=255)
                                                 tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg                                          >  2:3040434/1‑93 (MQ=255)
                                                  aTGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑TTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGt                                         <  2:1156108/93‑1 (MQ=255)
                                                   tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTTATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa                                        >  1:2063939/1‑93 (MQ=255)
                                                   tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa                                        >  2:5415146/1‑93 (MQ=255)
                                                   tGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTa                                        >  2:5915990/1‑93 (MQ=255)
                                                     ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                      <  1:5855353/93‑1 (MQ=255)
                                                     ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                      <  2:8326365/93‑1 (MQ=255)
                                                     ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                      >  1:4208855/1‑93 (MQ=255)
                                                     ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                      <  1:1513655/93‑1 (MQ=255)
                                                       cAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAct                                    <  1:1250709/93‑1 (MQ=255)
                                                           gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAt                                <  1:6190372/90‑1 (MQ=255)
                                                           gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAt                                <  2:3994583/90‑1 (MQ=255)
                                                            cgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAtt                               <  1:996724/91‑1 (MQ=255)
                                                             gcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGCCGACGCGGTGCCGCATTATCTGCGTACACTCTAttt                              >  2:8455543/2‑93 (MQ=255)
                                                              caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                             >  1:5671647/1‑93 (MQ=255)
                                                              caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                             <  1:7662466/93‑1 (MQ=255)
                                                              caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                             <  2:3853287/93‑1 (MQ=255)
                                                               accaGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAt                            >  2:7399377/1‑93 (MQ=255)
                                                                ccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATc                           <  1:7348387/93‑1 (MQ=255)
                                                                 caGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg                          <  2:1422909/93‑1 (MQ=255)
                                                                 caGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg                          >  2:8775552/1‑93 (MQ=255)
                                                                 caGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg                          >  2:4439672/1‑93 (MQ=255)
                                                                 caGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCg                          <  2:1556823/93‑1 (MQ=255)
                                                                  aGCGTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                         >  2:4946829/1‑93 (MQ=255)
                                                                     gTCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAAt                      >  2:2583938/1‑93 (MQ=255)
                                                                      tCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc                     >  2:562915/1‑93 (MQ=255)
                                                                      tCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc                     >  1:6393161/1‑93 (MQ=255)
                                                                      tCTGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATc                     >  1:5312971/1‑93 (MQ=255)
                                                                        tGAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                   <  2:7712628/93‑1 (MQ=255)
                                                                         gAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGtt                  >  2:2920629/1‑93 (MQ=255)
                                                                         gAAAGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGtt                  >  2:6843651/1‑93 (MQ=255)
                                                                          aaaGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTc                 <  2:7927283/93‑1 (MQ=255)
                                                                           aaGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCt                >  1:41615/1‑93 (MQ=255)
                                                                            aGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCtt               >  1:5076455/1‑93 (MQ=255)
                                                                            aGACCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCtt               >  1:8686534/1‑93 (MQ=255)
                                                                              aCCTTCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTa             <  1:841285/93‑1 (MQ=255)
                                                                                 ttCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt          >  1:5464851/1‑93 (MQ=255)
                                                                                 ttCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt          >  2:8548537/1‑93 (MQ=255)
                                                                                  tCATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTg         >  1:782915/1‑93 (MQ=255)
                                                                                   cATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTgg        >  2:5512623/1‑93 (MQ=255)
                                                                                   cATCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTgg        >  2:4706005/1‑93 (MQ=255)
                                                                                    aTCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGt       <  2:2714101/93‑1 (MQ=255)
                                                                                    aTCCCGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGt       <  1:3823434/93‑1 (MQ=255)
                                                                                       ccGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAg    <  1:1155436/93‑1 (MQ=255)
                                                                                        cGAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGt   <  1:2230207/93‑1 (MQ=255)
                                                                                         gAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGtt  >  2:7818729/1‑93 (MQ=255)
                                                                                         gAGCTGGAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGtt  >  1:6568773/1‑93 (MQ=255)
                                                                                              |                                                                                        
CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGAAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAA‑GTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT  >  NC_000913/4008434‑4008611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output Only 200 of 359 total aligned reads displayed.
CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGG‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  NC_000913/4008434‑4008618
                                                                                                  |                                                                                             
CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                                                               <  SRR4302541.5750722/94‑1 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGGCC‑GAG‑‑AAAGG‑CGGCAGTATCGGCAGTGCGCACCAGCGCCTGAAAGACCGCCATCCCGAGCT‑GG                                                                                              >  SRR4302541.1023062/1‑94 (MQ=19)
 GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302541.5683052/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302541.3844529/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302541.7466181/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302541.2630159/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              <  SRR4302541.1135414/94‑1 (MQ=60)
  GGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                                                             <  SRR4302541.4773953/94‑1 (MQ=60)
  GGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGGCTGAAAGACCTTCATCCCGAGCT‑GGA                                                                                             >  SRR4302541.1253601/1‑94 (MQ=60)
   GATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                                                                            <  SRR4302541.4692840/94‑4 (MQ=60)
   GATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Ggaa                                                                                            <  SRR4302541.1739807/94‑4 (MQ=60)
    ATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGTGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                            >  SRR4302541.6164569/1‑94 (MQ=60)
    ATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                            <  SRR4302541.7825424/94‑1 (MQ=60)
      GAACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTG                                                                                          <  SRR4302541.3463599/94‑1 (MQ=60)
       AACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                         >  SRR4302541.5256669/1‑94 (MQ=60)
       AACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                         >  SRR4302541.4035350/1‑94 (MQ=60)
       AACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                         <  SRR4302541.6947303/94‑1 (MQ=60)
       AACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                         <  SRR4302541.3754049/94‑1 (MQ=60)
       AACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                         >  SRR4302541.117051/1‑94 (MQ=60)
        ACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                                                        <  SRR4302541.3577844/94‑1 (MQ=60)
        ACGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                                                        <  SRR4302541.791493/94‑1 (MQ=60)
         CGACGCCGAAATGCTGTCGATGG‑CGGGG‑‑AAA‑GTCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGCGCTGGAAGTGATG                                                                                       >  SRR4302541.2559944/1‑94 (MQ=60)
         CGACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                                                       <  SRR4302541.4844192/94‑1 (MQ=60)
          GACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                      <  SRR4302541.8575370/94‑1 (MQ=60)
          GACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGGGATTG                                                                                      >  SRR4302541.810265/1‑94 (MQ=60)
           ACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                                                     >  SRR4302541.7686696/1‑94 (MQ=60)
           ACGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                                                     >  SRR4302541.5116742/1‑94 (MQ=60)
            CGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                                                                    <  SRR4302541.7060571/94‑1 (MQ=60)
            CGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                                                                    <  SRR4302541.7560387/94‑1 (MQ=60)
            CGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                                                                    <  SRR4302541.27915/94‑1 (MQ=60)
            CGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                                                                    <  SRR4302541.7661731/94‑1 (MQ=60)
            CGCCGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                                                                    >  SRR4302541.2460123/1‑94 (MQ=60)
               CGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302541.4866766/1‑94 (MQ=60)
               CGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302541.2532428/1‑94 (MQ=60)
               CGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 <  SRR4302541.8110775/94‑1 (MQ=60)
               CGAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCAGCCCGAGCTGGAAGTGATTGGTACT                                                                                 <  SRR4302541.8110776/94‑1 (MQ=60)
                GAAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                                                                >  SRR4302541.5149174/1‑94 (MQ=60)
                 AAATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                                                               >  SRR4302541.4213203/1‑94 (MQ=60)
                  AATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                              >  SRR4302541.3112378/1‑94 (MQ=60)
                   ATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             >  SRR4302541.2346873/1‑94 (MQ=60)
                   ATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             <  SRR4302541.3781178/94‑1 (MQ=60)
                   ATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             <  SRR4302541.767352/94‑1 (MQ=60)
                   ATGCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             >  SRR4302541.1176416/1‑94 (MQ=60)
                   ATGATGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             <  SRR4302541.4169162/94‑1 (MQ=60)
                     GCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                                                           <  SRR4302541.4794956/94‑1 (MQ=60)
                     GCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                                                           >  SRR4302541.3851607/1‑94 (MQ=60)
                     GCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                                                           >  SRR4302541.2686767/1‑94 (MQ=60)
                     GCTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTAGGGGCAGTGCGCACCAGCGTCTGAAAGCCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                                                           <  SRR4302541.5818630/94‑1 (MQ=57)
                      CTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302541.5466535/94‑1 (MQ=60)
                      CTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302541.108236/94‑1 (MQ=60)
                      CTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          >  SRR4302541.5374565/1‑94 (MQ=60)
                      CTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302541.2224400/94‑1 (MQ=60)
                      CTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302541.8020705/94‑1 (MQ=60)
                      CTGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302541.5277916/94‑1 (MQ=60)
                       TGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                                                         >  SRR4302541.4747699/1‑94 (MQ=60)
                       TGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                                                         <  SRR4302541.4792544/94‑1 (MQ=60)
                       TGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                                                         >  SRR4302541.8435980/1‑94 (MQ=60)
                       TGTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                                                         >  SRR4302541.7338821/1‑94 (MQ=60)
                        GTCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGAC                                                                        <  SRR4302541.158371/94‑1 (MQ=60)
                         TCGATGG‑C‑GGGGAAAG‑G‑CTGCGTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                                                       >  SRR4302541.4209090/1‑94 (MQ=60)
                         TCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                                                       >  SRR4302541.8355661/1‑94 (MQ=60)
                         TCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACG                                                                       <  SRR4302541.787464/94‑1 (MQ=60)
                         TCGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCAGAGCTGGAAGAGATTGGTACTAATGCCGACG                                                                       >  SRR4302541.2519927/1‑94 (MQ=57)
                          CGATGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGA                                                                      <  SRR4302541.5762265/94‑1 (MQ=60)
                             TGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                                                                   <  SRR4302541.2704292/94‑1 (MQ=60)
                             TGG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                                                                   >  SRR4302541.8045395/1‑94 (MQ=60)
                              GG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                                                                  <  SRR4302541.5185355/94‑1 (MQ=60)
                              GG‑C‑GGGGAAAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCG                                                                  <  SRR4302541.7072929/94‑1 (MQ=60)
                                   cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                <  SRR4302541.880388/85‑1 (MQ=60)
                                   cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                <  SRR4302541.1313323/85‑1 (MQ=60)
                                   cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                >  SRR4302541.6614086/10‑94 (MQ=60)
                                   cggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGG                                                                >  SRR4302541.4832652/10‑94 (MQ=60)
                                   GGG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGC                                                              >  SRR4302541.8063340/1‑94 (MQ=60)
                                    ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGCTTGGTACTAATGCCGACGACGCGGTG                                                               >  SRR4302541.348923/9‑94 (MQ=60)
                                    ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                               >  SRR4302541.6155967/9‑94 (MQ=60)
                                    ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                               >  SRR4302541.6450623/9‑94 (MQ=60)
                                    ggggaaagG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                               <  SRR4302541.6975569/86‑1 (MQ=60)
                                    GG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                                                             <  SRR4302541.6498876/94‑1 (MQ=60)
                                    GG‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                                                             <  SRR4302541.492899/94‑1 (MQ=60)
                                     G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                                                            >  SRR4302541.8609003/1‑94 (MQ=60)
                                     G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                                                            >  SRR4302541.4084572/1‑94 (MQ=60)
                                     G‑‑AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                                                            <  SRR4302541.8779656/94‑1 (MQ=60)
                                     G‑‑AAAGG‑CTGCATGATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCG                                                            <  SRR4302541.3267401/94‑1 (MQ=60)
                                        AAG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                          <  SRR4302541.3540161/94‑1 (MQ=60)
                                        AAAGG‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGCGATTGGTACTAATGCCGACGACGCGGTGCAGC                                                           >  SRR4302541.75432/1‑94 (MQ=60)
                                         AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                         >  SRR4302541.1661807/1‑94 (MQ=60)
                                         AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                         <  SRR4302541.584974/94‑1 (MQ=60)
                                         AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                         <  SRR4302541.6719161/94‑1 (MQ=60)
                                         AG‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAT                                                         >  SRR4302541.3718861/1‑94 (MQ=60)
                                          G‑G‑CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                        >  SRR4302541.7929982/1‑94 (MQ=60)
                                              CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                      <  SRR4302541.434258/94‑1 (MQ=60)
                                              CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                      >  SRR4302541.3392466/1‑94 (MQ=60)
                                              CTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAT                                                      <  SRR4302541.2758230/94‑1 (MQ=60)
                                               TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                     <  SRR4302541.1735716/94‑1 (MQ=60)
                                                GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    <  SRR4302541.6027773/94‑1 (MQ=60)
                                                GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGGGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCG                                                    >  SRR4302541.2881283/1‑94 (MQ=60)
                                                 CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   <  SRR4302541.1315080/94‑1 (MQ=60)
                                                 CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   <  SRR4302541.5086549/94‑1 (MQ=60)
                                                  ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATatcttcg                                                  >  SRR4302541.4714643/1‑87 (MQ=60)
                                                  ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  <  SRR4302541.3802315/94‑1 (MQ=60)
                                                  ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  <  SRR4302541.6391813/94‑1 (MQ=60)
                                                  ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  <  SRR4302541.5112738/94‑1 (MQ=60)
                                                   TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 <  SRR4302541.1420527/94‑1 (MQ=60)
                                                    TATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT                                                >  SRR4302541.4575865/1‑94 (MQ=60)
                                                    TATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGT                                                <  SRR4302541.4234179/94‑1 (MQ=60)
                                                     ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTTATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTA                                               >  SRR4302541.2083870/1‑94 (MQ=60)
                                                     ATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCANCCCGAGCTGGAAGTGATTGGTACTAATGCCGANGANNNGGNGCCGCATNNTNNNCNTA                                               >  SRR4302541.288693/1‑94 (MQ=60)
                                                      TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                              <  SRR4302541.6016003/94‑1 (MQ=60)
                                                      TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                              >  SRR4302541.7319384/1‑94 (MQ=60)
                                                       GGGCAGTGCGCCCCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             <  SRR4302541.4948649/94‑1 (MQ=60)
                                                       GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTTCTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             >  SRR4302541.6668725/1‑94 (MQ=60)
                                                       GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             <  SRR4302541.7773386/94‑1 (MQ=60)
                                                       GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             >  SRR4302541.4250709/1‑94 (MQ=60)
                                                        GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                            <  SRR4302541.5914894/94‑1 (MQ=60)
                                                        GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                            <  SRR4302541.1528729/94‑1 (MQ=60)
                                                         GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT                                           <  SRR4302541.91049/94‑1 (MQ=60)
                                                         GCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACT                                           <  SRR4302541.4143945/94‑1 (MQ=60)
                                                            gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGTCGACGCGGTGCCGCATTATCTGCGTAAACTCTT                                        >  SRR4302541.8558356/6‑94 (MQ=60)
                                                            gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTT                                        >  SRR4302541.7031907/6‑94 (MQ=60)
                                                            gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                        <  SRR4302541.3387933/89‑1 (MQ=60)
                                                            gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                        <  SRR4302541.4981384/89‑1 (MQ=60)
                                                            gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                        <  SRR4302541.5334719/89‑1 (MQ=60)
                                                             tgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                       <  SRR4302541.145225/90‑1 (MQ=60)
                                                             tgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                       <  SRR4302541.8535932/90‑1 (MQ=60)
                                                              gcgCACCAGCGTCTGAAAGCCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATT                                      <  SRR4302541.855919/91‑1 (MQ=60)
                                                              gcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATT                                      <  SRR4302541.5864424/91‑1 (MQ=60)
                                                               cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                     <  SRR4302541.1006000/92‑1 (MQ=60)
                                                               GGCACCAGGGTCCGAAAAACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                     <  SRR4302541.2290505/94‑1 (MQ=60)
                                                                GCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTA                                    <  SRR4302541.8188436/94‑1 (MQ=60)
                                                                GCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTA                                    >  SRR4302541.1878326/1‑94 (MQ=60)
                                                                GCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTA                                    >  SRR4302541.5729533/1‑94 (MQ=60)
                                                                 CACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   <  SRR4302541.1366647/94‑1 (MQ=60)
                                                                 CACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   <  SRR4302541.652154/94‑1 (MQ=60)
                                                                 CACCAGCGTCTGAAAGACCTACATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   <  SRR4302541.8691814/94‑1 (MQ=60)
                                                                  ACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGGTTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                  >  SRR4302541.2335410/1‑94 (MQ=60)
                                                                  ACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                  >  SRR4302541.120793/1‑94 (MQ=60)
                                                                   CCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCG                                 <  SRR4302541.7599271/94‑1 (MQ=60)
                                                                   CCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCG                                 <  SRR4302541.7426890/94‑1 (MQ=60)
                                                                   CCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCG                                 <  SRR4302541.6924764/94‑1 (MQ=60)
                                                                    CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGT                                >  SRR4302541.1266846/1‑94 (MQ=60)
                                                                     AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                               >  SRR4302541.7400554/1‑94 (MQ=60)
                                                                     AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                               >  SRR4302541.5722157/1‑94 (MQ=60)
                                                                        GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                            >  SRR4302541.4307402/1‑94 (MQ=60)
                                                                        GTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATC                            >  SRR4302541.6292880/1‑94 (MQ=60)
                                                                         TCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                           <  SRR4302541.192638/94‑1 (MQ=60)
                                                                         TCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                           >  SRR4302541.4275772/1‑94 (MQ=60)
                                                                         TCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                           <  SRR4302541.4907029/94‑1 (MQ=60)
                                                                           TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         >  SRR4302541.1860567/1‑94 (MQ=60)
                                                                            GAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTC                        >  SRR4302541.3209558/1‑94 (MQ=60)
                                                                             AAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCT                       >  SRR4302541.6796564/1‑94 (MQ=60)
                                                                             AAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCT                       <  SRR4302541.5512681/94‑1 (MQ=60)
                                                                             AAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCT                       <  SRR4302541.7120795/94‑1 (MQ=60)
                                                                              AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      <  SRR4302541.674545/94‑1 (MQ=60)
                                                                              AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      <  SRR4302541.2425961/94‑1 (MQ=60)
                                                                              AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      >  SRR4302541.8779499/1‑94 (MQ=60)
                                                                              AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      >  SRR4302541.6862274/1‑94 (MQ=60)
                                                                               AGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTT                     >  SRR4302541.5814495/1‑94 (MQ=60)
                                                                                GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    <  SRR4302541.1599813/94‑1 (MQ=60)
                                                                                GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    <  SRR4302541.6061387/94‑1 (MQ=60)
                                                                                GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    <  SRR4302541.3989865/94‑1 (MQ=60)
                                                                                 ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   <  SRR4302541.848915/94‑1 (MQ=60)
                                                                                 ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   <  SRR4302541.3292711/94‑1 (MQ=60)
                                                                                 ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   >  SRR4302541.2597288/1‑94 (MQ=60)
                                                                                  CCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                  <  SRR4302541.5271515/94‑1 (MQ=60)
                                                                                  CCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                  <  SRR4302541.7503049/94‑1 (MQ=60)
                                                                                   CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 >  SRR4302541.5520912/1‑94 (MQ=60)
                                                                                   CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 >  SRR4302541.5597297/1‑94 (MQ=60)
                                                                                   CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 >  SRR4302541.2516480/1‑94 (MQ=60)
                                                                                   CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 >  SRR4302541.733403/1‑94 (MQ=60)
                                                                                   CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 >  SRR4302541.3303382/1‑94 (MQ=60)
                                                                                    TTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG                >  SRR4302541.8883032/1‑94 (MQ=60)
                                                                                    TTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG                <  SRR4302541.206189/94‑1 (MQ=60)
                                                                                     TCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGG               >  SRR4302541.5972542/1‑94 (MQ=60)
                                                                                     TCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGG               >  SRR4302541.5972543/1‑94 (MQ=60)
                                                                                      CATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGT              <  SRR4302541.400930/94‑1 (MQ=60)
                                                                                      CATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGT              >  SRR4302541.2164111/1‑94 (MQ=60)
                                                                                       ATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTC             >  SRR4302541.7782167/1‑94 (MQ=60)
                                                                                        TCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCA            >  SRR4302541.825016/1‑94 (MQ=60)
                                                                                         CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           <  SRR4302541.227521/94‑1 (MQ=60)
                                                                                         CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           <  SRR4302541.3727942/94‑1 (MQ=60)
                                                                                         CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           <  SRR4302541.5365406/94‑1 (MQ=60)
                                                                                          CCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGT          <  SRR4302541.3435009/94‑1 (MQ=60)
                                                                                           CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         >  SRR4302541.2328941/1‑94 (MQ=60)
                                                                                           CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         >  SRR4302541.6635975/1‑94 (MQ=60)
                                                                                           CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         <  SRR4302541.8369066/94‑1 (MQ=60)
                                                                                           CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         <  SRR4302541.2251808/94‑1 (MQ=60)
                                                                                           CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         <  SRR4302541.300882/94‑1 (MQ=60)
                                                                                            GAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTG        >  SRR4302541.6475033/1‑94 (MQ=60)
                                                                                            GAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTG        >  SRR4302541.3696938/1‑94 (MQ=60)
                                                                                             AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT       >  SRR4302541.257617/1‑94 (MQ=60)
                                                                                             AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT       >  SRR4302541.1346738/1‑94 (MQ=60)
                                                                                             AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATATGGTCAGTTGT       >  SRR4302541.7348118/1‑94 (MQ=60)
                                                                                               ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     <  SRR4302541.4292682/91‑1 (MQ=60)
                                                                                               ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     >  SRR4302541.3949676/4‑94 (MQ=60)
                                                                                               ctgGAAGTGATTGGTACTAATGCCGACGACGCGGGGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     >  SRR4302541.838516/4‑94 (MQ=60)
                                                                                                G‑GAAGTGATTGGTACTAATGCCGACGACTCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC   <  SRR4302541.7708688/94‑1 (MQ=60)
                                                                                                G‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC   <  SRR4302541.4535343/94‑1 (MQ=60)
                                                                                                  GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  SRR4302541.4706067/1‑94 (MQ=60)
                                                                                                  GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  SRR4302541.7089066/1‑94 (MQ=60)
                                                                                                  GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  <  SRR4302541.7016129/94‑1 (MQ=60)
                                                                                                  |                                                                                             
CGGGATGAACGACGCCGAAATGCTGTCGATGG‑C‑GGG‑‑AAA‑G‑CTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  NC_000913/4008434‑4008618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑