Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I2 R1 884 257.2 12958910 96.9% 12557183 93.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,008,525 +G intergenic (+92/‑141) b4555 → / → yigM hypothetical protein/hypothetical protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,008,5241.G99.6% 856.8 / ‑4.3 242intergenic (+91/‑142)b4555/yigMhypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base G (139/102);  total (139/103)
Fisher's exact test for biased strand distribution p-value = 4.26e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 258 total aligned reads displayed.

CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT  >  NC_000913/4008434‑4008611
                                                                                              |                                                                                       
 gggATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑g                                                                                        <  2:3208939/93‑1 (MQ=255)
  ggATGAACGACGCCGAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑gg                                                                                       >  1:1546250/1‑92 (MQ=255)
       aaCGACGCCGAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTg                                                                                   >  2:6162777/1‑93 (MQ=255)
          gacgCCGAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGCGAtt                                                                                >  2:675891/1‑93 (MQ=255)
           acgCCGAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTg                                                                               >  2:5834659/1‑93 (MQ=255)
            cgCCGAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTgg                                                                              <  1:5366461/93‑1 (MQ=255)
             gCCGAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGt                                                                             <  2:5885363/93‑1 (MQ=255)
              ccGAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                                                            <  1:4051091/93‑1 (MQ=255)
              ccGAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTa                                                                            >  2:3383752/1‑93 (MQ=255)
               cGAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAc                                                                           <  1:4649788/93‑1 (MQ=255)
                gAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                                                          <  2:3161757/93‑1 (MQ=255)
                gAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                                                          >  2:4819552/1‑93 (MQ=255)
                gAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                                                          >  1:3349964/1‑93 (MQ=255)
                gAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                                                          >  2:5126113/1‑93 (MQ=255)
                gAAATGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACt                                                                          >  1:1971070/1‑93 (MQ=255)
                 aaaTGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTa                                                                         <  1:237725/93‑1 (MQ=255)
                  aaTGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTaa                                                                        <  2:795188/93‑1 (MQ=255)
                   aTGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                                                       >  1:52960/1‑93 (MQ=255)
                   aTGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAt                                                                       <  2:38543/93‑1 (MQ=255)
                    tGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                                                      >  1:4146173/1‑93 (MQ=255)
                    tGCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATg                                                                      >  1:3041742/1‑93 (MQ=255)
                     gCTGTCGATGGCGGGG‑AAATGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                                                                     >  2:2720312/1‑93 (MQ=255)
                     gCTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGc                                                                     <  1:1050943/93‑1 (MQ=255)
                      cTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc                                                                    <  1:4084567/93‑1 (MQ=255)
                      cTGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGcc                                                                    <  2:2232111/93‑1 (MQ=255)
                       tGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                                                   >  1:1407707/1‑93 (MQ=255)
                       tGTCGATGGCGGGG‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCcg                                                                   <  1:6470595/93‑1 (MQ=255)
                            aTGGC‑GGGTAAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAGCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCgacgac                                                              <  1:3603057/93‑1 (MQ=255)
                              ggcgggg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                                                            >  1:2807597/5‑93 (MQ=255)
                              ggcgggg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAcgc                                                            <  2:3351338/89‑1 (MQ=255)
                                ggggg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCgg                                                          <  1:2700719/91‑1 (MQ=255)
                                 gggg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGt                                                         >  2:2513508/2‑93 (MQ=255)
                                  ggg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTg                                                        >  1:5004402/1‑93 (MQ=255)
                                   gg‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGc                                                       >  1:2517117/1‑93 (MQ=255)
                                       aaggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGc                                                    >  1:2791377/4‑93 (MQ=255)
                                        aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGCCCTTCATCCCGAGCTGGAAGTGATTGGTTCTAATGCAGAAGACGCGGTGCCGCa                                                   <  1:5090415/91‑1 (MQ=255)
                                        aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                                                   >  2:4577567/3‑93 (MQ=255)
                                        aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                                                   >  1:1704730/3‑93 (MQ=255)
                                        aggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCa                                                   >  2:5084695/3‑93 (MQ=255)
                                         ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                                                  >  2:742423/2‑93 (MQ=255)
                                         ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                                                  >  2:5687741/2‑93 (MQ=255)
                                         ggCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAt                                                  <  1:2995812/92‑1 (MQ=255)
                                          gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt                                                 >  1:2244095/1‑93 (MQ=255)
                                          gCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCAtt                                                 <  1:3137867/93‑1 (MQ=255)
                                            tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt                                               <  2:2964203/93‑1 (MQ=255)
                                            tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt                                               >  2:4294798/1‑93 (MQ=255)
                                            tGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTAt                                               >  2:2066694/1‑93 (MQ=255)
                                             gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                              >  1:245660/1‑93 (MQ=255)
                                             gCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATc                                              >  1:6065662/1‑93 (MQ=255)
                                              cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                             <  2:283182/93‑1 (MQ=255)
                                              cATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCt                                             <  2:3007050/93‑1 (MQ=255)
                                               aTTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTg                                            <  1:3679/93‑1 (MQ=255)
                                                ttATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGc                                           <  1:3852043/93‑1 (MQ=255)
                                                 tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg                                          >  2:3586052/1‑93 (MQ=255)
                                                 tATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCg                                          <  2:5530256/93‑1 (MQ=255)
                                                    gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa                                       <  1:4564449/93‑1 (MQ=255)
                                                    gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa                                       >  1:4465521/1‑93 (MQ=255)
                                                    gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa                                       >  1:4370626/1‑93 (MQ=255)
                                                    gggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaa                                       >  1:5432929/1‑93 (MQ=255)
                                                     ggCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTaaa                                      >  2:1906761/1‑93 (MQ=255)
                                                          tgcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTa                                 <  1:4304671/89‑1 (MQ=255)
                                                          tgcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTa                                 <  1:5542915/89‑1 (MQ=255)
                                                          tgcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTa                                 <  1:570214/89‑1 (MQ=255)
                                                           gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAt                                <  1:234217/90‑1 (MQ=255)
                                                           gcgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAt                                <  1:3364982/90‑1 (MQ=255)
                                                            cgcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAtt                               <  1:5946110/91‑1 (MQ=255)
                                                             gcaccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAttt                              <  2:5646869/92‑1 (MQ=255)
                                                              caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                             >  1:5739202/1‑93 (MQ=255)
                                                              caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                             >  2:2290427/1‑93 (MQ=255)
                                                              caccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTa                             <  1:5971873/93‑1 (MQ=255)
                                                               accaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAt                            <  1:4716140/93‑1 (MQ=255)
                                                                ccaGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATc                           >  2:1751236/1‑93 (MQ=255)
                                                                  aGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                         >  2:4281516/1‑93 (MQ=255)
                                                                  aGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                         >  2:1401544/1‑93 (MQ=255)
                                                                  aGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGt                         <  2:5958357/93‑1 (MQ=255)
                                                                   gCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTa                        <  1:407104/93‑1 (MQ=255)
                                                                   gCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTa                        >  1:4419988/1‑93 (MQ=255)
                                                                    cGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTaa                       <  2:6378342/93‑1 (MQ=255)
                                                                       cTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTCTTTATCGTAATCg                    <  1:6353272/93‑1 (MQ=255)
                                                                        tGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                   >  1:1767071/1‑93 (MQ=255)
                                                                        tGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGt                   >  2:1294276/1‑93 (MQ=255)
                                                                           aaGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCt                <  2:2438483/93‑1 (MQ=255)
                                                                            aGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCtt               >  2:1184272/1‑93 (MQ=255)
                                                                            aGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCtt               <  2:591470/93‑1 (MQ=255)
                                                                             gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCttt              >  2:847556/1‑93 (MQ=255)
                                                                             gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCttt              >  2:2866516/1‑93 (MQ=255)
                                                                             gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCttt              <  2:2561730/93‑1 (MQ=255)
                                                                             gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCttt              <  2:5199948/93‑1 (MQ=255)
                                                                             gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCttt              >  1:2423098/1‑93 (MQ=255)
                                                                             gACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCttt              >  1:3768360/1‑93 (MQ=255)
                                                                              aCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCtttt             >  1:2306490/1‑92 (MQ=255)
                                                                                 ttCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt          >  2:4419812/1‑93 (MQ=255)
                                                                                 ttCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt          <  1:4938798/93‑1 (MQ=255)
                                                                                 ttCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAttt          <  2:1677221/93‑1 (MQ=255)
                                                                                   cATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTgg        >  2:4118175/1‑93 (MQ=255)
                                                                                      cccGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCa     <  1:769312/93‑1 (MQ=255)
                                                                                      cccGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCa     >  2:5359694/1‑93 (MQ=255)
                                                                                       ccGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAg    >  1:4759430/1‑93 (MQ=255)
                                                                                         gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGtt  >  1:3966265/1‑93 (MQ=255)
                                                                                         gAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGtt  >  2:1108987/1‑93 (MQ=255)
                                                                                              |                                                                                       
CGGGATGAACGACGCCGAAATGCTGTCGATGGC‑GGG‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT  >  NC_000913/4008434‑4008611

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  NC_000913/4008434‑4008618
                                                                                               |                                                                                             
CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                                                               >  SRR4302534.5120675/1‑94 (MQ=60)
CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑G                                                                                               >  SRR4302534.4137848/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              >  SRR4302534.1586773/1‑94 (MQ=60)
 GGGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GG                                                                                              <  SRR4302534.5778724/94‑1 (MQ=60)
  GGATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GGA                                                                                             >  SRR4302534.413263/1‑94 (MQ=60)
    ATGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAG                                                                                            <  SRR4302534.6072789/94‑1 (MQ=60)
     TGAACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGT                                                                                           >  SRR4302534.4101236/1‑94 (MQ=60)
       AACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGA                                                                                         >  SRR4302534.1340804/1‑94 (MQ=60)
        ACGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGAT                                                                                        <  SRR4302534.2476150/94‑1 (MQ=60)
         CGACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATT                                                                                       >  SRR4302534.3098112/1‑94 (MQ=60)
          GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                      >  SRR4302534.4172744/1‑94 (MQ=60)
          GACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTG                                                                                      <  SRR4302534.4566139/94‑1 (MQ=60)
           ACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                                                     <  SRR4302534.215487/94‑1 (MQ=60)
           ACGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGG                                                                                     >  SRR4302534.3816131/1‑94 (MQ=60)
            CGCCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGT                                                                                    <  SRR4302534.5511102/94‑1 (MQ=60)
              CCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                                                                  >  SRR4302534.4448491/1‑94 (MQ=60)
              CCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                                                                  <  SRR4302534.4159739/94‑1 (MQ=60)
              CCGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTAC                                                                                  <  SRR4302534.6146366/94‑1 (MQ=60)
               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTCTGGGCAATCCGCCCCAGCGTCTGAAAGACCTTCATCTCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302534.5461671/1‑94 (MQ=26)
               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302534.2022668/1‑94 (MQ=60)
               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302534.6212755/1‑94 (MQ=60)
               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 >  SRR4302534.3439663/1‑94 (MQ=60)
               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACT                                                                                 <  SRR4302534.4776016/94‑1 (MQ=60)
               CGAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGCGCGCACTAGCGTCTGAAAGACCTTTATCCCCAGCTGGTAGTGCTTGGTACT                                                                                 >  SRR4302534.1665812/1‑94 (MQ=23)
                GAAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTA                                                                                >  SRR4302534.3441984/1‑94 (MQ=60)
                 AAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                                                               >  SRR4302534.3957355/1‑94 (MQ=60)
                 AAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                                                               >  SRR4302534.3542398/1‑94 (MQ=60)
                 AAATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAA                                                                               <  SRR4302534.243944/94‑1 (MQ=60)
                  AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                              >  SRR4302534.54380/1‑94 (MQ=60)
                  AATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAAT                                                                              >  SRR4302534.4181944/1‑94 (MQ=60)
                   ATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             >  SRR4302534.3121531/1‑94 (MQ=60)
                   ATGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATG                                                                             >  SRR4302534.4258082/1‑94 (MQ=60)
                    TGCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGC                                                                            >  SRR4302534.1629423/1‑94 (MQ=60)
                     GCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                                                           >  SRR4302534.5006458/1‑94 (MQ=60)
                     GCTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                                                           <  SRR4302534.1078154/94‑1 (MQ=60)
                      tctgtggatgggggggaaagggtgcattaagggcagtgccgcCCAGCGTCTGAAAGGCCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCC                                                                           <  SRR4302534.3946029/52‑1 (MQ=57)
                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302534.4194240/94‑1 (MQ=60)
                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          >  SRR4302534.1444730/1‑94 (MQ=60)
                      CTGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCG                                                                          <  SRR4302534.5468422/94‑1 (MQ=60)
                       TGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                                                         <  SRR4302534.6644884/94‑1 (MQ=60)
                       TGTCGATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGA                                                                         >  SRR4302534.2829878/1‑94 (MQ=60)
                          CGATGGCGGGGAAAG‑GCTGCATTATGGGCAGGGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTCATGCCGACGA                                                                      >  SRR4302534.1486913/1‑94 (MQ=57)
                           GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     <  SRR4302534.286975/94‑1 (MQ=60)
                           GATGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGAC                                                                     <  SRR4302534.6129864/94‑1 (MQ=60)
                            ATGGCGGGTAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGAGCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACG                                                                    <  SRR4302534.3699943/94‑1 (MQ=60)
                             TGGCGGGGAAAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGC                                                                   >  SRR4302534.2881431/1‑94 (MQ=60)
                               GGGGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                <  SRR4302534.2772061/94‑1 (MQ=60)
                                gcggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGG                                                                 >  SRR4302534.5204516/11‑94 (MQ=60)
                                 cggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGT                                                                >  SRR4302534.2955382/10‑94 (MQ=60)
                                 GGG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGC                                                              >  SRR4302534.2583954/1‑94 (MQ=60)
                                  ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTG                                                               >  SRR4302534.5139687/9‑94 (MQ=60)
                                  ggggaaagGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑Gggggagagtgtttctaataaacgcgacgagcgg                                                              >  SRR4302534.5255105/9‑61 (MQ=60)
                                  GG‑‑AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCC                                                             <  SRR4302534.5921882/94‑1 (MQ=60)
                                      AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATTCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCT                                                          >  SRR4302534.3353768/1‑94 (MQ=60)
                                      AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                          >  SRR4302534.4997272/1‑94 (MQ=60)
                                      AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                          >  SRR4302534.2407346/1‑94 (MQ=60)
                                      AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                          >  SRR4302534.748747/1‑94 (MQ=60)
                                      AAG‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCA                                                          >  SRR4302534.1749025/1‑94 (MQ=60)
                                      AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                                                           >  SRR4302534.3660162/1‑94 (MQ=60)
                                      AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                                                           >  SRR4302534.2864792/1‑94 (MQ=60)
                                      AAAGGCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGC                                                           >  SRR4302534.4512792/1‑94 (MQ=60)
                                        G‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                        >  SRR4302534.2303568/1‑94 (MQ=60)
                                        G‑GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATT                                                        <  SRR4302534.3074145/94‑1 (MQ=60)
                                          GCTGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTA                                                       <  SRR4302534.3220387/94‑1 (MQ=60)
                                            TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                     >  SRR4302534.252087/1‑94 (MQ=60)
                                            TGCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATC                                                     >  SRR4302534.6230581/1‑94 (MQ=60)
                                             GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    <  SRR4302534.4786377/94‑1 (MQ=60)
                                             GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    <  SRR4302534.3745434/94‑1 (MQ=60)
                                             GCATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCT                                                    <  SRR4302534.2946458/94‑1 (MQ=60)
                                              CATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTG                                                   >  SRR4302534.791757/1‑94 (MQ=60)
                                               ATTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGC                                                  <  SRR4302534.3786/94‑1 (MQ=60)
                                                TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 <  SRR4302534.3955476/94‑1 (MQ=60)
                                                TTATGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCG                                                 <  SRR4302534.315434/94‑1 (MQ=60)
                                                   TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                              >  SRR4302534.4586267/1‑94 (MQ=60)
                                                   TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                              >  SRR4302534.5579208/1‑94 (MQ=60)
                                                   TGGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAA                                              >  SRR4302534.4488391/1‑94 (MQ=60)
                                                    GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             >  SRR4302534.4192441/1‑94 (MQ=60)
                                                    GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             >  SRR4302534.651571/1‑94 (MQ=60)
                                                    GGGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAA                                             <  SRR4302534.4688196/94‑1 (MQ=60)
                                                     GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                            <  SRR4302534.5891129/94‑1 (MQ=60)
                                                     GGCAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAAC                                            <  SRR4302534.4042837/94‑1 (MQ=60)
                                                       CAGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTC                                          >  SRR4302534.909550/1‑94 (MQ=60)
                                                        AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT                                         <  SRR4302534.1546667/94‑1 (MQ=60)
                                                        AGTGCGCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCT                                         <  SRR4302534.15771/94‑1 (MQ=60)
                                                         gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                        <  SRR4302534.2873123/89‑1 (MQ=60)
                                                         gtgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTA                                        <  SRR4302534.2400431/89‑1 (MQ=60)
                                                          tgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                       <  SRR4302534.584204/90‑1 (MQ=60)
                                                          tgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                       <  SRR4302534.4609680/90‑1 (MQ=60)
                                                          tgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                       <  SRR4302534.5692536/90‑1 (MQ=60)
                                                          tgcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTAT                                       <  SRR4302534.4421359/90‑1 (MQ=60)
                                                           tcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATT                                      >  SRR4302534.2592787/4‑94 (MQ=60)
                                                           gcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATT                                      <  SRR4302534.240351/91‑1 (MQ=60)
                                                           gcgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATT                                      <  SRR4302534.3455110/91‑1 (MQ=60)
                                                            cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                     >  SRR4302534.5704319/3‑94 (MQ=60)
                                                            cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                     >  SRR4302534.5540578/3‑94 (MQ=60)
                                                            cgCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTT                                     <  SRR4302534.6107554/92‑1 (MQ=60)
                                                             GCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTA                                    >  SRR4302534.1923142/1‑94 (MQ=60)
                                                             GCACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTA                                    >  SRR4302534.5894739/1‑94 (MQ=60)
                                                              CACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   >  SRR4302534.4857053/1‑94 (MQ=60)
                                                              CACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTAT                                   <  SRR4302534.6134068/94‑1 (MQ=60)
                                                               ACCAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATC                                  <  SRR4302534.4844205/94‑1 (MQ=60)
                                                                 CAGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGA                                >  SRR4302534.4230491/1‑94 (MQ=60)
                                                                  AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                               >  SRR4302534.4539319/1‑94 (MQ=60)
                                                                  AGCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTA                               >  SRR4302534.3153836/1‑94 (MQ=60)
                                                                   GCGTCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAA                              <  SRR4302534.417511/94‑1 (MQ=60)
                                                                      TCTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCG                           <  SRR4302534.6283890/94‑1 (MQ=60)
                                                                       CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTCTTTATCGTAATCGT                          <  SRR4302534.6524849/94‑1 (MQ=60)
                                                                       CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          >  SRR4302534.240083/1‑94 (MQ=60)
                                                                       CTGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGT                          >  SRR4302534.1812959/1‑94 (MQ=60)
                                                                        TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         >  SRR4302534.2579693/1‑94 (MQ=60)
                                                                        TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         <  SRR4302534.2431579/94‑1 (MQ=60)
                                                                        TGAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTT                         >  SRR4302534.2488014/1‑94 (MQ=60)
                                                                         GAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTC                        >  SRR4302534.2258602/1‑94 (MQ=60)
                                                                         GAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTC                        >  SRR4302534.1793938/1‑94 (MQ=60)
                                                                         GAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTC                        <  SRR4302534.3269082/94‑1 (MQ=60)
                                                                         GAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTC                        >  SRR4302534.329786/1‑94 (MQ=60)
                                                                         GAAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGACGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTT                        >  SRR4302534.3914589/1‑94 (MQ=60)
                                                                          AAAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCT                       <  SRR4302534.2813035/94‑1 (MQ=60)
                                                                           AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      >  SRR4302534.4758898/1‑94 (MQ=60)
                                                                           AAGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTT                      >  SRR4302534.179072/1‑94 (MQ=60)
                                                                            AGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTT                     >  SRR4302534.2487537/1‑94 (MQ=60)
                                                                            AGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTT                     >  SRR4302534.3869535/1‑94 (MQ=60)
                                                                            AGACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTT                     <  SRR4302534.519514/94‑1 (MQ=60)
                                                                             GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTT                    >  SRR4302534.2367714/1‑94 (MQ=60)
                                                                             GACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTA                    <  SRR4302534.4507144/94‑1 (MQ=60)
                                                                              ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   >  SRR4302534.4393921/1‑94 (MQ=60)
                                                                              ACCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTAT                   >  SRR4302534.1565344/1‑94 (MQ=60)
                                                                               CCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                  >  SRR4302534.5675813/1‑94 (MQ=60)
                                                                               CCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                  >  SRR4302534.249888/1‑94 (MQ=60)
                                                                               CCTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATT                  <  SRR4302534.4886496/94‑1 (MQ=60)
                                                                                CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 <  SRR4302534.2356063/94‑1 (MQ=60)
                                                                                CTTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTT                 >  SRR4302534.5431111/1‑94 (MQ=60)
                                                                                 TTCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTG                <  SRR4302534.5072615/94‑1 (MQ=60)
                                                                                  TCATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGG               >  SRR4302534.2639307/1‑94 (MQ=60)
                                                                                   CATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGT              >  SRR4302534.5624286/1‑94 (MQ=60)
                                                                                   CATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGT              >  SRR4302534.379567/1‑94 (MQ=60)
                                                                                    ATCCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTC             >  SRR4302534.2345243/1‑94 (MQ=60)
                                                                                      CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           >  SRR4302534.4888667/1‑94 (MQ=60)
                                                                                      CCCGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAG           <  SRR4302534.788446/94‑1 (MQ=60)
                                                                                        CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         >  SRR4302534.4072435/1‑94 (MQ=60)
                                                                                        CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         <  SRR4302534.4704929/94‑1 (MQ=60)
                                                                                        CGAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTT         >  SRR4302534.4549861/1‑94 (MQ=60)
                                                                                         GAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTG        <  SRR4302534.4359563/94‑1 (MQ=60)
                                                                                         GAGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTG        <  SRR4302534.71901/94‑1 (MQ=60)
                                                                                          AGCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGT       <  SRR4302534.4605260/94‑1 (MQ=60)
                                                                                           GCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTC      <  SRR4302534.3863596/94‑1 (MQ=60)
                                                                                           GCTGGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTC      <  SRR4302534.207105/94‑1 (MQ=60)
                                                                                            ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     >  SRR4302534.654245/4‑94 (MQ=60)
                                                                                            ctgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCA     <  SRR4302534.1175416/91‑1 (MQ=60)
                                                                                             tgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAA    >  SRR4302534.6054134/3‑94 (MQ=60)
                                                                                             tgGAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAA    >  SRR4302534.3452802/3‑94 (MQ=60)
                                                                                             G‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAAC   >  SRR4302534.3447662/1‑94 (MQ=60)
                                                                                               GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  SRR4302534.6188874/1‑94 (MQ=60)
                                                                                               |                                                                                             
CGGGATGAACGACGCCGAAATGCTGTCGATGGCGGG‑‑AAA‑GCTGCATTATGGGCAGTGGCCACCAGCGTCTGAAAGACCTTCATCCCGAGCT‑GAAGTGATTGGTACTAATGCCGACGACGCGGTGCCGCATTATCTGCGTAAACTCTATTTATCGTAATCGTTCTTTATTTGGTCAGTTGTCAACC  >  NC_000913/4008434‑4008618

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑